scholarly journals Kandungan Fitokimia, Potensi Antibakteri dan Antioksidan Hasil Ekstraksi Caulerpa racemosa dengan Pelarut Berbeda

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Endar Marraskuranto ◽  
Muhammad Nursid ◽  
Swestri Utami ◽  
Iriani Setyaningsih ◽  
Kustiariyah Tarman
Keyword(s):  
Escherichia Coli ◽  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Inhibitory Concentration ◽  
Ethyl Acetate Extract ◽  
Caulerpa Racemosa ◽  
Antioxidant Power ◽  
E Coli ◽  
Antibacterial Assay ◽  
Ferric Reducing Antioxidant Power

Caulerpa racemosa merupakan rumput laut hijau yang mengandung senyawa aktif yang berpotensi sebagai antibakteri dan antioksidan. Perbedaan profil fitokimia dan bioaktivitas suatu ekstrak dipengaruhi oleh kepolaran pelarut ekstraksi. Penelitian ini bertujuan untuk mempelajari profil fitokimia, aktivitas antibakteri dan antioksidan ekstrak C. racemosa. Ekstrak diperoleh dari sampel segar C. racemosa yang dimaserasi menggunakan pelarut n-heksana, etil asetat, dan metanol. Uji antibakteri dilakukan dengan metode Resazurin Microtitter Assay (REMA) terhadap bakteri Escherichia coli dan Staphylococcus aureus. Aktivitas antioksidan diuji menggunakan metode 1,1-diphenyl-2-picrylhydrazyl (DPPH) dan Ferric Reducing Antioxidant Power (FRAP). Hasil penapisan fitokimia memperlihatkan bahwa ekstrak n-heksana mengandung alkaloid dan triterpenoid, sedangkan ekstrak etil asetat dan metanol mengandung alkaloid, flavonoid, tanin, fenol, dan steroid. Aktivitas antibakteri terbaik ditunjukkan oleh ekstrak etil asetat dengan nilai Minimum Inhibitory Concentration (MIC) terhadap E. coli sebesar 250 mg/mL. Ekstrak etil asetat C. racemosa juga memperlihatkan aktivitas antioksidan terbaik dengan nilai IC50 DPPH sebesar 110,7 mg/mL dan nilai FRAP sebesar 96,68 mmol Fe (II)/g. ABSTRACTCaulerpa racemosa is a green seaweed that contains active compounds that are potential for antibacterial and antioxidant. The phytochemical constituents and bioactivity of an extract depend on the extraction solvent polarity. This study aimed to determine the phytochemical profiles, antibacterial and antioxidant activity of C. racemosa extracts. Extracts were obtained from the fresh C. racemosa samples that were macerated with n-hexane, ethyl acetate, and methanol. Antibacterial assay was tested by Resazurin Microtiter Assay (REMA) method against Escherichia coli and Staphylococcus aureus. Antioxidant activity was determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and Ferric Reducing Antioxidant Power (FRAP) method. Phytochemical screening showed that n-hexane extract contained alkaloid and triterpenoid, while ethyl acetate and methanol extracts contained alkaloids, flavonoids, tannins, phenols, and steroids. The most active extract in the antibacterial assay was ethyl acetate extract with a Minimum Inhibitory Concentration (MIC) value of 250 mg/mL against E. coli. Ethyl acetate extract of C. racemosa also showed the best antioxidant activity where the IC50 of DPPH value was 110.7 mg/mL and the FRAP value was 96.68 mmol Fe(II)/g.

scholarly journals PENENTUAN KADAR FENOLAT TOTAL DAN AKTIVITAS ANTIOKSIDAN DARI EKSTRAK DAUN DADAP MERAH (Erythrina fusca Lour) SECARA SPEKTROFOTOMETRI UV-Vis

2019 ◽  
Vol 11 (1) ◽  
pp. 09-16
Author(s):  
Epi Supri Wardi ◽  
Zulkarni R Zulkarni R ◽  
Desy Nurdianti
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Phenolic Content ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Hexane Extract ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power

Determination of total phenolate and antioxidant activity of red leaf extract (Erythrina fusca Lour) was done by UV-Vis spectrophotometry. This study aims to determine the total phenolic content and antioxidant activity of hexane, ethyl acetate and ethanol extract. The extracts were prepared using a non-polar-maseration method with hexane, ethyl acetate and ethanol solvents. The results showed total phenolic concentration using the Folin-Ciocalteu method were 0.412 g/100 g in the hexane extract, 1.782 g/100 g in the ethyl acetate extract and 5.455 g/100 g in the ethanol extract. Antioxidant activity conducted by using FRAP method (Ferric Reducing Antioxidant Power) were obtained 0,682 mmol Fe (II)/100 g at hexane extract, 5,186 mmol Fe (II)/100 g at ethyl acetate extract and 10,591 mmol Fe (II)/100 g on the ethanol extract. The antioxidant activity of gallic acid as standard was 44.356 g mmol Fe (II)/100g.

scholarly journals PENENTUAN KADAR FENOLAT TOTAL DAN AKTIVITAS ANTIOKSIDAN DARI EKSTRAK DAUN DADAP MERAH (Erythrina fusca Lour) SECARA SPEKTROFOTOMETRI UV-Vis

2019 ◽  
Vol 11 (1) ◽  
pp. 09-16
Author(s):  
Epi Supri Wardi ◽  
Zulkarni R Zulkarni R ◽  
Desy Nurdianti
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Phenolic Content ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Hexane Extract ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power

Determination of total phenolate and antioxidant activity of red leaf extract (Erythrina fusca Lour) was done by UV-Vis spectrophotometry. This study aims to determine the total phenolic content and antioxidant activity of hexane, ethyl acetate and ethanol extract. The extracts were prepared using a non-polar-maseration method with hexane, ethyl acetate and ethanol solvents. The results showed total phenolic concentration using the Folin-Ciocalteu method were 0.412 g/100 g in the hexane extract, 1.782 g/100 g in the ethyl acetate extract and 5.455 g/100 g in the ethanol extract. Antioxidant activity conducted by using FRAP method (Ferric Reducing Antioxidant Power) were obtained 0,682 mmol Fe (II)/100 g at hexane extract, 5,186 mmol Fe (II)/100 g at ethyl acetate extract and 10,591 mmol Fe (II)/100 g on the ethanol extract. The antioxidant activity of gallic acid as standard was 44.356 g mmol Fe (II)/100g.

scholarly journals Antibacterial and Antioxidant Activities of Derriobtusone A Isolated fromLonchocarpus obtusus

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Mayron Alves Vasconcelos ◽  
Francisco Vassiliepe Sousa Arruda ◽  
Daniel Barroso de Alencar ◽  
Silvana Saker-Sampaio ◽  
Maria Rose Jane Ribeiro Albuquerque ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Pathogenic Bacteria ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Test Results ◽  
Dpph Radical ◽  
Antioxidant Power ◽  
E Coli ◽  
Planktonic Growth ◽  
Ferric Reducing Antioxidant Power

This study evaluated the effect of derriobtusone A, a flavonoid isolated fromLonchocarpus obtusus, on two important pathogenic bacteria,Staphylococcus aureusandEscherichia coli, as well as its antioxidant activity and toxicity. Planktonic growth assays were performed, and the inhibition of biofilm formation was evaluated. In addition, antioxidant activity was assessed by DPPH radical scavenging assay, ferrous ion chelating assay, ferric-reducing antioxidant power assay, andβ-carotene bleaching assay. Toxicity was evaluated by the brine shrimp lethality test. Results showed that derriobtusone A completely inhibited the planktonic growth ofS. aureusat 250 and 500 μg/mL; however, it did not have the same activity onE. coli. Derriobtusone A reduced the biomass and colony-forming unit (cfu) ofS. aureusbiofilm at concentrations of 250 and 500 μg/mL. In various concentrations, it reduced the biofilm biomass ofE. coli, and, in all concentrations, it weakly reduced the cfu. Derriobtusone A showed highly efficient antioxidant ability in scavenging DPPH radical and inhibitingβ-carotene oxidation. The compound showed no lethality toArtemiasp. nauplii. In conclusion, derriobtusone A may be an effective molecule againstS. aureusand its biofilm, as well as a potential antioxidant compound with no toxicity.

scholarly journals Antibacterial Activity of Liverworts of Dumortiera hirsute (Sw.) Nees Ethyl Acetate Extract Against Pathogenic Bacteria

2021 ◽  
Vol 9 (2) ◽  
pp. 75
Author(s):  
Luthfiah Luthfiah ◽  
Dwi Setyati ◽  
Sattya Arimurti
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Antibacterial Properties ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
E Coli ◽  
Agar Well Diffusion Method

Dumortiera hirsuta is one of the liverworts that can be used as a medicinal to prevent infection by pathogenic bacteria. The content of secondary metabolites of D. hirsuta has potential as antibacterial properties includes flavonoids, alkaloids and steroids. This research is to analyze the antibacterial activity of moss D. hirsuta against pathogenic bacteria that will be beneficial to humans. Liverworts of D. hirsuta were extracted using ethyl acetate solvent and tested against three types of pathogenic bacteria using the agar well-diffusion method. The results of this study indicated that the ethyl acetate extract of D. hirsuta at 100% concentration could inhibit the growth of Escherichia coli, Staphylococcus aureus, and Salmonella typhi bacteria. The range of antibacterial activity categories of the ethyl acetate extract of D. hirsuta to E. coli, S. aureus, and S. typhi between weak to moderate.

scholarly journals ASSESSMENT OF ANTIOXIDANT ACTIVITIES, PHENOL AND FLAVONOID CONTENTS OF DIFFERENT EXTRACTS OF LEAVES, BARK AND ROOT FROM THE Abutilon indicum (L.) SWEET

2017 ◽  
Vol 10 (4) ◽  
pp. 88
Author(s):  
Adaikalam Raj ◽  
Saranya D ◽  
Sekar J
Keyword(s):  
Free Radical ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Phenol ◽  
Butylated Hydroxytoluene ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Abutilon Indicum

Objective: This study evaluated the antioxidant properties of petroleum ether, chloroform, ethyl acetate and methanol extracts of Abutilon indicum (L).Methods: The different extracts of A. indicum leaves, bark and roots  were antioxidant potential by using 1,1-diphenyl-2-picryl-hydrazyl (DPPH), 2, 2-Azino-bis-3-ethyl benzthiazoline-6-sulphonic acid (ABTS•+) , Hydrogen peroxide scavenging, Superoxide anions scavenging,  Hydroxyl radical scavenging, Ferric reducing antioxidant power, Total antioxidant activity (Phosphomolybdic acid) and total phenol and flavonoid contents.                      Results: The highest total phenol and flavonoid contents in leaves extracts of A. indicum. The total phenol (3.08 ± 0.06) mg/ml and flavonoid (7.16 ± 0.15) mg/ml were found to be higher in ethyl acetate extract of A. indicum and free radical scavenging activities IC50 values were calculated and compare to standard for L- ascorbic acid, (BHT) butylated hydroxytoluene and gallic acid    Conclusion:  The results of the study revealed that the ethyl acetate extract of A. indicum leaves can be used for the biological characterization and importance of the compounds identified and creates a platform to screen many bioactive compounds to treat many diseases.Keywords: Abutilon indicum, Free radical scavenging activity, 1,1-diphenyl-2-picryl-hydrazyl, 2-azino-bis-3-ethyl benzthiazoline-6-sulfonic acid,Ferric reducing antioxidant power.

scholarly journals Optimum Conditions for Extraction of Antibacterial Compounds from Citrus Aurantifolia Fruit Peel Waste

2018 ◽  
Vol 14 (1) ◽  
pp. 34-39
Author(s):  
Rima Munawaroh
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Citrus Fruit ◽  
Optimum Conditions ◽  
Fruit Peel ◽  
Fresh Sample ◽  
Citrus Aurantifolia ◽  
E Coli ◽  
Antibacterial Assay ◽  
Fruit Processing

Citrus fruit peel is a major waste in citrus fruit processing industry. The research on extraction activecompounds of Citrus aurantifolia (lime) fruit peel waste and antibacterial activity assay has been done. Theaim of research was to get optimum condition to extract their active compounds which have antibacterialactivity. The dried lime fruit peel was extracted by maceration method using ethanol 48%, 72%, and 96%.The dried and fresh lime fruit peel were also extracted using ethyl acetate. Antibacterial assay was done bydiffusion agar against Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922. The resultshowed that optimal condition to extract antibacterial compound using fresh sample with ethyl acetate assolvent. The ethyl acetate extract of fresh sample was more active against S. aureus than E. coli.

scholarly journals Antioxidant activities of Dichrostachys cinerea (L.) Wight et Arn (Leguminosae): Correlations between the Polyphenol Level and the Antioxidant Activity

2020 ◽  
pp. 1-11
Author(s):  
Reine Raïssa Rolande Aworet Samseny ◽  
Line-Edwige Mengome ◽  
Sophie Aboughe Angone
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethyl Acetate ◽  
Antiradical Activity ◽  
Antioxidant Activities ◽  
Natural Antioxidants ◽  
Dpph Radical ◽  
Dichrostachys Cinerea ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power

Aims: Free radicals are involved in many diseases. Antioxidants help control them. The overproduction of free radicals or reduction of natural antioxidants promotes the destruction of cells in our body, for example in neurodegenerative diseases. The aim of our study is to evaluate the correlation between the level of polyphenol and antioxidant activity of Dichrostachys cinerea barks he work was carried out at the phytochemistry department of the Institute of Pharmacopoeia and Traditional Medicine (Gabon). Methodology: Different extracts of Dichrostachys cinerea barks were obtained with polarity solvent (dichloromethane, ethyl acetate, butanol, methanol and water).  The antioxidant activity is evaluating using respectively FRAP method (Ferric Reducing Antioxidant Power), antiradical activity by the method of inhibition of DPPH radical (2.2-diphenyl-1-picrylhydrazyl), cations ABTS radical (2.2'-azinobis-[3-acid-6-sulfonic ethylenzothiazoline]). Results: Ethyl acetate and dichloromethane extract have the higher value for polyphenolic compounds, respectively 52,27±0,66 mg EAG / g and 49,72±0,55 mg EAG / g. Aqueous extract have the lower value 20,67±1,05 mg EAG / g. The different fractions of Dichrostachys cinerea have antioxidant effect, and this effect is in correlation with its constituents: polyphenols, tannins and flavonoids. Results were compared to standard antioxidants such as ascorbic acid and quercetin. Conclusion: This study confirms higher is the level of polyphenols greater is the antioxidant power.

scholarly journals Radical Scavenging activity and phytochemical screening of the leaf extract of Synsepalum dulcificum (Schumach. & Thonn.) Daniell

2021 ◽  
Vol 10 (3) ◽  
pp. 069-076
Author(s):  
Awotedu BF ◽  
Omolola TO ◽  
Akala AO ◽  
Ogunsiji AO ◽  
Owoeye EA ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Antioxidant Power ◽  
Extract Concentration ◽  
Total Antioxidant ◽  
Ferric Reducing Antioxidant Power

Antioxidants are chemicals that possess bioactive constituents which usually allow them to fight against free radicals. Antioxidant and phytochemicals properties of the leaves of Synsepalum dulcificum were investigated using ethyl-acetate, ethanol and methanol extracts. 2, 2-diphenyl-picrylhydrazyl (DPPH) scavenging activity, total antioxidant activity, ferric reducing antioxidant power (FRAP), total flavonoid and the total phenolic content was carried out. The phytochemical screening assay was done on the following: Alkaloids, saponin, flavonoids, tannin, anthraquinones, terpenoids, cardiac glycosides, and phlobatannin using the test solvent extracts. The results gotten for antioxidant revealed that methanol gave the highest extract yield (17.4%) while ethyl-acetate extract had the least yield (5.0%). At 250 μg/ml extract concentration, the methanolic extract gave the highest amount with the IC50 values (89.94 μg/ml), followed by ethanol extract (68.20 μg/ml)) while ethyl acetate extract had the least scavenging activity of (39.21 μg/ml), and these were significantly different (P<0.05). Total antioxidant activity (ascorbic acid equivalent) at 250 μg/ml of S. dulcificum ranged from 41.7 AAE in ethyl-acetate extract to 58.98 AAE in methanol extract. Furthermore, ferric reducing antioxidant power (FRAP) of the leaf extracts revealed that at 250 μg/ml extract concentration, methanol extract had the highest (0.29μmolFe2+/g) reduction potential, followed by ethanol extract (0.28μmolFe2+/g) and ethyl-acetate extract (0.26μmolFe2+/g). The total phenolic content of the extracts ranged from 0.02μg/ml TAE–0.09μg/ml TAE at 1000μg/ml extract concentration while the total flavonoid content ranged from 0.32mg/g QE in ethyl-acetate extract to 14.38mg/g QE in methanol extract. This study suggests that S. dulcificum extract exhibit great potential antioxidant ability that could fight against oxidative stress.

In Vitro Antibacterial Activity of Different Polar Solvent Extracts from Oxalis

2014 ◽  
Vol 1033-1034 ◽  
pp. 240-246
Author(s):  
Xue Jing Qiu ◽  
Ying Zi He ◽  
Xiao Wang
Keyword(s):  
Escherichia Coli ◽  
Ethyl Acetate ◽  
Minimal Inhibitory Concentration ◽  
Inhibitory Activity ◽  
Inhibitory Concentration ◽  
Polar Solvent ◽  
Ethyl Acetate Extract ◽  
Antimicrobial Effect ◽  
Chloroform Extract ◽  
Butanol Extract

The Oxalis extract was obtained by soaking with ethanol, then mixed with distilled water and successively extracted using chloroform, ethyl acetate and n-butanol. The four different polar solvent (chloroform, ethyl acetate, n-butanol, aqueous) extracts were evaporated under reduced pressure to obtain four kinds of crude extractum. Antibacterial activity on Staphylococcus aureus, Escherichia coli, Albicans Saccharomyces and Pseudomonas aeruginosa of the four fractions were studied by minimal inhibitory concentration method (MIC). The results indicated that the chloroform extract and n-butanol extract from red Oxalis have no antimicrobial activity to the above 4 bacteria; the ethyl acetate extract showed significant inhibitory activities to the above 4 bacteria, the minimal inhibitory concentration (MIC) were 2560ug/ml, 1280ug/ml, 2560ug/ml, 2560ug/ml respectively; the aqueous extract have inhibitory activity to Escherichia coli and Albicans Saccharomyces, the minimal inhibitory concentration (MIC) were 2560ug/ml, 2560ug/ml respectively, but it has no antimicrobial effect on the other two kinds of bacteria. The chloroform extract and ethyl acetate extract from yellow Oxalis have no antimicrobial activity to the above 4 bacteria; the n-butanol extract has inhibitory activity to Escherichia coli, Albicans Saccharomyces and Pseudomonas aeruginosa, the minimal inhibitory concentration (MIC) were 5120ug/ml, 5120ug/ml, 5120ug/ml respectively, but it has no antimicrobial effect on Staphylococcus aureus; the aqueous extract have inhibitory activity to Escherichia coli and Albicans Saccharomyces, the minimal inhibitory concentration (MIC) were 2560ug/ml, 5120ug/ml respectively, but it has no antimicrobial effect on the other two kinds of bacteria.

scholarly journals Antimicrobial Activity of Allium chinense G. Don.

2017 ◽  
Vol 2 (3) ◽  
pp. 129-138
Author(s):  
Frans Grovy Naibaho ◽  
Maria Bintang ◽  
Fachriyan Hasmi Pasaribu
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Microbial Growth ◽  
Inhibitory Concentration ◽  
Ethyl Acetate Extract ◽  
Salmonella Typhi ◽  
Antimicrobial Activities ◽  
Biologically Active ◽  
E Coli ◽  
Allium Chinense

This study aims to analyze antimicrobial activity of Allium chinense G. Don extract against Eschericia coli, Salmonella typhi, Staphylococcus aureus, Bacillus subtilis and Candida albicans, and to examine the active compounds. Allium chinense G. Don was extracted using maseration method and treated with ethanol 70% (v/v), ethanol 96% (v/v), ethyl acetate, n-hexane, and aquadest. Antimicrobial activity assay was conducted using agar difusion method and compounds analysis using Gas chromatography-mass­spectrometry (GC-MS). Antimicrobial activities assay showed that all extracts could inhibit microbial growth. Ethyl acetate extract has the highest antimicrobial activity against all the microbial test. Minimum inhibitory concentration (MIC) of ethyl acetate extract against C. albicans, B. subtilis, E. coli, S. aureus, S. typhi was 25, 100, 250, 250, 1000 mg ml-1 respectively. As many as 25 compounds were derived from GC-MS analysis and most of them were known as the antimicrobial compounds. This study revealed that Allium chinense G. Don contains biologically active compunds as antimicrobial agent particularly anti Candida.

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