scholarly journals SYNTHESIS AND ANTIBACTERIAL ACTIVITY OF COMPOSITE BASED ON CHITOSAN-GRAFTED-(N-BUTYL ACRYLATE) AND SILVER NANOPARTICLES

2019 ◽  
Vol 57 (3) ◽  
pp. 309
Author(s):  
La Thi Thai Ha ◽  
Chau Ngoc Mai
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Butyl Acrylate ◽  
Chemical Reduction ◽  
Antibacterial Activities ◽  
Butyl Hydroperoxide ◽  
Grafting Efficiency ◽  
Tert Butyl Hydroperoxide ◽  
Long Time ◽  
Grafting Copolymerization

It has been found for a long time that chitosan (CS) and silver nanoparticles (AgNPs) have outstanding antibacterial activities but there were some drawbacks restricting their wide utilization. In this research, CS modified is combined with AgNPs to expand applications and enhance the antibacterial activities. The colloid of CS and AgNPs (CS/Ag) was synthesized via chemical reduction while grafting copolymerization was carried out with monomer n-butyl acrylate (BA) and initiator tert-butyl hydroperoxide (TBHP) generating composite between CS-g-BA/Ag. The effects of parameters on synthesizing CS-g-BA/Ag composite were studied by determining the grafting percentage (G%) and grafting efficiency (E%).

scholarly journals Interaction of Silver Nanoparticles with Serum Proteins Affects Their Antimicrobial ActivityIn Vivo

2013 ◽  
Vol 57 (10) ◽  
pp. 4945-4955 ◽  
Author(s):  
Divya Prakash Gnanadhas ◽  
Midhun Ben Thomas ◽  
Rony Thomas ◽  
Ashok M. Raichur ◽  
Dipshikha Chakravortty
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Serum Albumin ◽  
Serum Proteins ◽  
Antibacterial Properties ◽  
Antibacterial Activities ◽  
Resistant Bacteria ◽  
Human Society ◽  
Vis Spectroscopy

ABSTRACTThe emergence of multidrug-resistant bacteria is a global threat for human society. There exist recorded data that silver was used as an antimicrobial agent by the ancient Greeks and Romans during the 8th century. Silver nanoparticles (AgNPs) are of potential interest because of their effective antibacterial and antiviral activities, with minimal cytotoxic effects on the cells. However, very few reports have shown the usage of AgNPs for antibacterial therapyin vivo. In this study, we deciphered the importance of the chosen methods for synthesis and capping of AgNPs for their improved activityin vivo. The interaction of AgNPs with serum albumin has a significant effect on their antibacterial activity. It was observed that uncapped AgNPs exhibited no antibacterial activity in the presence of serum proteins, due to the interaction with bovine serum albumin (BSA), which was confirmed by UV-Vis spectroscopy. However, capped AgNPs [with citrate or poly(vinylpyrrolidone)] exhibited antibacterial properties due to minimized interactions with serum proteins. The damage in the bacterial membrane was assessed by flow cytometry, which also showed that only capped AgNPs exhibited antibacterial properties, even in the presence of BSA. In order to understand thein vivorelevance of the antibacterial activities of different AgNPs, a murine salmonellosis model was used. It was conclusively proved that AgNPs capped with citrate or PVP exhibited significant antibacterial activitiesin vivoagainstSalmonellainfection compared to uncapped AgNPs. These results clearly demonstrate the importance of capping agents and the synthesis method for AgNPs in their use as antimicrobial agents for therapeutic purposes.

scholarly journals Modifying chitosan with n-butyl acrylate - antibacterial ability

2019 ◽  
Vol 2 (2) ◽  
pp. 12-18
Author(s):  
Ha Thi Thai La ◽  
Mai Ngoc Chau
Keyword(s):  
Acetic Acid ◽  
Glass Transition ◽  
Butyl Acrylate ◽  
Antibacterial Agent ◽  
Natural Polymers ◽  
Tert Butyl Hydroperoxide ◽  
Wide Range ◽  
The World ◽  
Grafting Copolymerization ◽  
Lower Glass Transition Temperature

Polymer deriving from nature as Chitosan, which is one of the most abundant natural polymers in the world has effectively antibacterial potentiality. However, chitosan is relatively difficult for applications due to its disadvantageous properties. In this article, modifying chitosan (CS) is the grafting copolymerization using monomer n-butyl acrylate (BA) and tert-butyl hydroperoxide (TBHP) as an initiator. This process purposes to attain product having lower glass transition temperature (Tg) than original chitosan causing a wide range of applications but still keeping the capability of being an excellently antibacterial agent. The effects of parameters on synthesizing copolymer were studied by determining the grafting percentage (G%) and grafting efficiency (E%). The results revealed that the highest G = 242% and E = 61% were obtained at the conditions as following: BA/CS = 4 w/w, TBHP/CS = 5 v/w with [TBHP] = 20mM, [CS] = 0.75% w/v in acetic acid 0.6% and reaction temperature 90°C in 6 hours.

scholarly journals Green Synthesis of Silver Nanoparticles Using Different Plant Materials and Their Antibacterial Activity

2018 ◽  
Vol 6 (4) ◽  
pp. 294-301
Author(s):  
Dipesh Shahi ◽  
Elisha Bhattarai ◽  
Milan Poudel ◽  
Prarthana Pradhan ◽  
Raja Ram Pradhananga ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Cost Effective ◽  
Antibacterial Activities ◽  
Ag Nps ◽  
Plant Materials ◽  
Green Route ◽  
Sem Study ◽  
Varied Size ◽  
Physical And Chemical

The green route of metal nanoparticles synthesis has received significant attention in recent years due to it's cost-effective, non-toxic and eco-friendly nature in comparison to other physical and chemical methods. This study reports on the synthesis of silver nanoparticles (Ag-NPs) from bio-reduction of 1mM aqueous silver nitrate (AgNO3) by extracts prepared from three different plants namely, Brassica oleracea L. var. italica Plenck (Broccoli), Capsicum annuum L. (Chili) and Parthenium hysterophorus L. (Carrot grass). The synthesized Ag-NPs were characterized using UV- visible spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). Ag-NPs synthesized showed the surface plasmon resonance with the appearance of absorption peaks around the range of 410-430 nm. The possible biomolecules involved in the reduction and the stabilization of synthesized Ag-NPs were found to be alcoholic, phenolic, amine and carbonyl groups. SEM study revealed that Ag-NPs were spherical in shape with varied size about 10-40 nm. Besides, the analysis of antioxidant and antibacterial activities of Ag-NPs was carried out. The Ag-NPs synthesized using B. oleracea extract showed the higher antioxidant activity than Ag-NPs synthesized from both C. annuum & P. hysterophorus extracts. Ag-NPs exhibited good antibacterial activity against both Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria. The higher antibacterial activity was shown by Ag-NPs synthesized from P. hysterophorus extract in comparison to Ag-NPs synthesized from both C. annuum & B. oleracea extracts. Hence, it can be concluded that Ag-NPs synthesized following the green route could be the source for potential antioxidant and antibacterial agents. Int. J. Appl. Sci. Biotechnol. Vol 6(4): 294-301

scholarly journals Enhancement of the antibacterial potential of plantaricin by incorporation into silver nanoparticles

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Sara Adel Amer ◽  
Hala Mohamed Abushady ◽  
Rasha Mohamed Refay ◽  
Mahmoud Ahmed Mailam
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Foodborne Pathogens ◽  
Inhibitory Activity ◽  
Pathogenic Bacteria ◽  
Antibacterial Activities ◽  
Stability Period ◽  
Genes Encoding ◽  
Pharmaceutical Industries ◽  
Antibacterial Potential

Abstract Background Bacteriocins are proteinaceous compounds produced from lactic acid bacteria. Bacteriocins are well-known for their antibacterial potential and safety for application in food. However, the commercial availability of bacteriocin is facing several limitations; among them is the low yield and short stability period. That calls for a new strategy for overcoming these hurdles. Among these approaches is incorporating bacteriocin in nanoparticles. So, the aim of this study was to enhance the plantaricin produced from isolated Lactobacillus plantarum strain using nanotechnology. Results In this study, the plnEF genes encoding plantaricin EF have been identified and sequenced (accession number of MN172264.1). The extracted bacteriocin (EX-PL) was obtained by the ammonium sulfate method. Then, it was used for biosynthesizing plantaricin-incorporated silver nanoparticles (PL-SNPs). The synthesized nanoparticles were confirmed by SEM-EDAX analysis. The antibacterial activity of both combined (PL-SNPs) and extracted plantaricin (EX-PL) were tested against some strains of foodborne pathogenic bacteria. The results revealed that the antibacterial activities were increased by 99.2% on the combination of bacteriocin with the silver nanoparticle. The MIC of EX-PL (7.6 mg/mL) has been lowered after incorporating into silver nanoparticles and reached 0.004 mg/mL for PL-SNPs. Despite that extracted plantaricin showed no inhibitory activity towards Listeria monocytogenes, plantaricin-incorporated silver nanoparticles displayed inhibitory activity against this strain. Furthermore, the stability period at 4 °C was increased from 5 days to 60 days for EX-PL and PL-SNPs, respectively. Conclusions Plantaricin-incorporated silver nanoparticles possess higher antibacterial activity and more stability than the free one, which makes it more fitting for combating foodborne pathogens and open more fields for applications in both food and pharmaceutical industries. Graphical abstract

scholarly journals Green synthesis, characterization and antibacterial activities of silver nanoparticles from strawberry fruit extract

2017 ◽  
Vol 19 (4) ◽  
pp. 128-136 ◽  
Author(s):  
Saviour A. Umoren ◽  
Alexis M. Nzila ◽  
Saravanan Sankaran ◽  
Moses M. Solomon ◽  
Peace S. Umoren
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Room Temperature ◽  
Size Range ◽  
Antibacterial Activities ◽  
Inhibitory Effect ◽  
Strawberry Fruit ◽  
Fruit Extract ◽  
Vis Spectroscopy ◽  
Better Than

Abstract Silver nanoparticles (AgNPs) have been synthesized in the presence of Strawberry fruit extract (SBFE) at room temperature. The synthesized AgNPs was characterized by UV-vis spectroscopy, SEM, EDS, XRD, TEM and FTIR. The UV-vis spectra of the AgNPs show SPR band at 450 nm. TEM results indicate that AgNPs are spherical in shape and size range between 7–65 nm. Antibacterial activity of the synthesized AgNPs has been assessed against Pseudomonas aeruginosa and Bacillus licheniformis. The results show that AgNPs exhibit inhibitory effect and effect is a function of AgNPs concentration. The antibacterial activity of the prepared AgNPs has been compared with two antibiotics, amoxicillin and ciprofloxacin. It is found that the antibiotics perform better than AgNPs.

Biosynthesis of silver nanoparticles using Bacillus subitilis and its antibacterial activity

2021 ◽  
Vol 30 (2) ◽  
pp. 67-71
Author(s):  
Ghada S.M. Abd el wahab
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Antibacterial Activities ◽  
Bacterial Strains ◽  
Standard Strain ◽  
Physical Chemical ◽  
Inhibition Zones ◽  
Reference Strains ◽  
And Staphylococcus Aureus

Background: Silver nanoparticles (AgNPs) have recently been extensively investigated because of their superior physical, chemical, and biological characteristics, and their superiority is primarily due to the size, shape, composition, crystallinity, and structure of AgNPs compared to their bulk forms. Objective: The current study aimed to investigate the antibacterial activity of silver nanoparticles (AgNPs) synthesized intracellular by using standard strain Bacillus subitilis ATCC 6633 against reference strains Escherichia coli ATCC 2592 and Staphylococcus aureus ATCC 29737. Results: The synthesized AgNPs showed potent antibacterial activities against the two tested bacterial strains with inhibition zones ranged from 42 -52mm and MIC 27.2 µg / ml. The silver nanoparticles were characterized with particle size ≃ 100 nm and zeta potential -19. There was deformation in both tested strains upon treatment with AgNPs which was observed by Scanning Electron Microscopy (SEM). Conclusion: The results indicated that AgNPs could be used as an effective antibacterial agent.

scholarly journals Coating of Polyetheretherketone Films with Silver Nanoparticles by a Simple Chemical Reduction Method and Their Antibacterial Activity

Coatings ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 91 ◽  
Author(s):  
Andrés Cruz-Pacheco ◽  
Deysi Muñoz-Castiblanco ◽  
Jairo Gómez Cuaspud ◽  
Leonel Paredes-Madrid ◽  
Carlos Parra Vargas ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Metallic Nanoparticles ◽  
Chemical Reduction ◽  
Single Layer ◽  
Polymeric Substrate ◽  
Chemical Route ◽  
Force Microscopy ◽  
Transmission Electron ◽  
Cellular Replication

The coating of polymeric substrate polyetheretherketone (PEEK) with silver nanoparticles (AgNPs) was carried out by a wet chemical route at room temperature. The coating process was developed from the Tollens reagent and D-glucose as reducing agent. The resulting composite exhibited antimicrobial activity. The PEEK films were coated with a single layer and two layers of silver nanoparticles in various concentrations. The crystallographic properties of the polymer and the silver nanoparticles were analyzed by X-ray diffraction (XRD). Fourier transform infrared spectra (FTIR) show the interaction between the silver nanoparticles with the polymeric substrate. Transmission electron microscope (TEM) images confirmed the obtaining of metallic nanoparticles with average sizes of 25 nm. It was possible to estimate the amount of silver deposited on PEEK with the help of thermogravimetric analysis. The morphology and shape of the AgNPs uniformly deposited on the PEEK films was ascertained by the techniques of scanning electron microscopy (SEM) and atomic force microscopy (AFM), evidencing the increase in the amount of silver by increasing the concentration of the metal precursor. Finally, the antibacterial activity of the films coated with Ag in Escherichia coli, Serratia marcescens and Bacillus licheniformis was evaluated, evidencing that the concentration of silver is crucial in the cellular replication of the bacteria.

scholarly journals Synthesis and Comparative Antibacterial Activity of Fatty Acid Capped Silver Nanoparticles

2020 ◽  
Vol 14 (3) ◽  
pp. 1941-1947
Author(s):  
Rama Sharma
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Fatty Acid ◽  
Room Temperature ◽  
Reduction Method ◽  
Chemical Reduction ◽  
Inert Atmosphere ◽  
Chemical Reduction Method ◽  
Visible Spectroscopy ◽  
E Coli

In the present work, the chemical reduction method has been used to synthesize silver nanoparticles using stearic acid capping agents in different concentrations. These nanoparticles are characterized by ultraviolet-visible spectroscopy and dynamic light scattering (DLS). The size of the synthesized silver nanoparticles found between 80-100 nm and stable up to 5 months. These nanoparticles show a very good bactericidal influence on E. coli and S. aureus. In this method silver nanoparticles have synthesized at room temperature without using any inert atmosphere, this is the advantage of this method over others.

scholarly journals Silver Nanoparticles Obtained by Aqueous or Ethanolic Aloe vera Extracts: An Assessment of the Antibacterial Activity and Mercury Removal Capability

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Ederley Vélez ◽  
Gloria Campillo ◽  
Gladis Morales ◽  
César Hincapié ◽  
Jaime Osorio ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Environmental Protection Agency ◽  
Chemical Reduction ◽  
Aloe Vera ◽  
Agar Plate ◽  
Size Control ◽  
Ethanolic Extract ◽  
World Health ◽  
Mercury Removal

Silver nanoparticles (AgNPs) were synthesized by chemical reduction of Ag+ ions (from silver nitrate AgNO3), using aqueous or ethanolic Aloe vera extracts as reducing, stabilizing, and size control agent. The nanoparticles’ sizes were between 2 and 7 nm for ethanolic extract and between 3 and 14 nm for aqueous extract, as measured by High-Resolution Transmission Electron Microscope (HRTEM). The antibacterial activity against a mesophilic microorganism, Kocuria varians, a Gram-positive coccus, was measured by counting bacterial colonies in agar plate for both extracts. We found that 4% effective concentration is the lowest concentration that completely inhibited visible growth. Mercury removal was investigated by Atomic Absorption Spectroscopy (AAS) measurements, where it was shown that it is not necessary to use high concentrations of nanoparticles for effective removal of mercury inasmuch as with a 20% V/V concentration of both extracts; the Hg(II) removal percentage was above 95%. These results show that the mercury remaining unremoved from the different essays is below the level allowed by World Health Organization (WHO) and the Environmental Protection Agency (EPA).

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