RELATIONSHIPS OF TEMPERATURE AND HUMIDITY TO THE BIODEGRADATION OF PETROLEUM HYDROCARBONS IN SOILS
This work focused on monitoring CO2production, microbial growth and residual hydrocarbon concentration during bioremediation experiments performed on laboratory soil microcosms. A natural soil was artificially contaminated with hexadecane and adjusted with inorganic nutrients to stimulate biodegradation. Microbial growth, CO2production and residual hexadecane were periodically monitored at different soil water contents ranging from 0.15 to 0.25 g water g_1 of dry soil and at different temperatures ranging from 20 to 25oC. Results showed that the humidity has a greater effect on microbial activity and contaminant degradation than the temperature. The study established the experimental regression equation of temperature and humidity to the hexadecane mineralization rate, an important parameter in assessing the ability to convert organic carbon into inorganic carbon. The difference between the results of the hexadecane mineralization rate obtained from the experiment and calculated from the regression equation is not too high, from 2% to 20%.