scholarly journals Effect of seed hydro-priming durations on germination and seedling growth of bitter gourd (Momordica charantia)

PLoS ONE ◽  
2021 ◽  
Vol 16 (8) ◽  
pp. e0255258
Author(s):  
Binod Adhikari ◽  
Pankaj Raj Dhital ◽  
Sambat Ranabhat ◽  
Hari Poudel

The yield and quality of an annual crop are highly dependent on uniform and rapid germination of the seeds. In case of bitter gourd (Momordica charantia), the germination and field emergence is always a problem as seeds have thick and hard seed coat. Pre-sowing hydro-priming is one of the most suitable, affordable, easily available, and cost-effective techniques in breaking down seed dormancy to enhance germination. Hence, a field experiment was conducted in Surkhet district of Nepal 2020 to assess the effect of different hydro-priming duration on germination and seedling growth of bitter gourd. The experiment was laid out in single factor Randomized Complete Block Design (RCBD) with four replications and seven treatments including different hydro-priming durations (T0: control, T1: 6 hours, T2: 12 hours, T3: 18 hours, T4: 24 hours, T5: 36 hours and T6: 48 hours) of bitter gourd seeds of Palee variety, the most popular variety among the farmers. The highest water uptake and germination were found in 48 hours of seed hydro-primed seeds whereas the lowest water uptake and germination were observed on non-primed seeds. Similarly, the tallest seedling, most vigorous seedling in terms of seedling vigour index I and II was observed in 48 hours hydro-primed seeds followed by 36 hours of seed hydro-priming and shortest seedling and the least vigorous seedling in control. Thus 48 hours of seed hydro-priming was found to be effective for increasing germination and seedling growth in bitter gourd, which needs to be further investigated under large, open-field conditions with different varieties.

2019 ◽  
Vol 2 (1) ◽  
pp. 265-273 ◽  
Author(s):  
Avinash Shrestha ◽  
Shreena Pradhan ◽  
Jenny Shrestha ◽  
Mahesh Subedi

Seed priming enhances early seed emergence and proper crop stand establishment which enables the crop to capture more soil moisture, nutrients, and solar radiation. An on-farm experiment was conducted in Okhaldhunga, Nepal to study the effect of six different priming treatments (50 millimoles common salt solution, 200 millimoles urea solution, 1 percent MOP solution, 2.5 times diluted urine, water, and control) on germination and growth of two maize varieties, Manakamana-3 and Nutan-IL60. The experiment was set up in factorial randomized complete block design with three replications. Various germination traits: germination percent, seedling vigour, germination index, mean germination time, and seedling growth traits: shoot length, root length, and dry root weight, were studied. Nitrogen-based (Urea and Urine) priming resulted the most desirable change for the studied traits. Nitrogen-based priming also had significant positive effect on stress tolerance related traits like root length and dry root weight.


2016 ◽  
Vol 11 (4) ◽  
pp. 246 ◽  
Author(s):  
Semra Kilic ◽  
Hatice Tuğba Aca

Soil salinity is a serious threat to agricultural ecological environment and agriculture sustainability. Ever increasing salinity negatively affects processes such as plant growth and development, ultimately causing diminished economic yield and quality of production, and it might cause a worldwide famine in the future. Thus, helping plants adapt to saline soils and increasing their yield and quality is a must. Our study focused on the enhancing role of exogenously applied folic acid (FA) in mitigation of toxicity caused by salt (NaCl). Barley seeds were pre-treated with 50 <span>µ</span>M FA for 24 h and then exposed to salt. Morphological and anatomical changes in seed germination and seedling growth stages were compared between different treatments of salt in laboratory conditions. Adverse effects of salt in both germination and seedling growth stages depended on the concentration of salt treatment (0.0, 0.25, 0.275, 0.30, 0.325 and 0.35 M). It was shown that the application of FA effectively alleviated the salt-induced inhibition, and reduced the negative effects of salt on germination (germination index and vigour index), seedling growth (radicle and coleoptile lengths, fresh weight) and leaf (stomata and epidermis number, stomatal index, stomata sizes of adaxial and abaxial surfaces) parameters. Moreover, FA elevated all examined parameters of barley also under non-stress conditions. Especially, germination and vigour indices were significantly higher than the control. Our results suggest that exogenous FA is involved in the resistance of barley to salt-stress.


2013 ◽  
Vol 31 (3) ◽  
pp. 501-509 ◽  
Author(s):  
H.H. Ali ◽  
A. Tanveer ◽  
M.A. Nadeem ◽  
M.M. Javaid ◽  
M.S. Kashif ◽  
...  

Experiments were conducted to evaluate the allelopathic influence of Rhynchosia capitata on germination and seedling growth of mungbean (Vigna radiate) along with identification of the phytotoxic substances responsible for this activity. Water extracts of root, shoot, leaf, fruit and whole plant were prepared by soaking them in water in a ratio of 1:20 (w/v) for 24 h. All the extracts affected germination and seedling growth of mungbean, but higher inhibition was seen with R. capitata leaf water extracts. A linear decrease in the germination characteristics of mungbean was observed with the decrease in the concentration of leaf extract from 5% to 1%. The soil-incorporated residues (1-4% w/w) of R. capitata stimulated the growth of root and hypocotyl at low concentrations, while it inhibited their growth at higher concentrations. Rhynchosia capitata soil-incorporated residues (4% w/w) significantly reduced the seedling vigour index of mungbean in addition to their significant effect on total germination. A significant amount of water-soluble phenolic acids were found in R. capitata plant extracts. The content of total phenolic acids was higher in the leaf extract compared to that of the stem, fruit or root extracts. Two phenolic acids including vanillic acid and 4‑(hydroxymethyl) benzoic acid were found in R. capitata leaf extracts.


2021 ◽  
Vol 9 (1) ◽  
pp. 2980-2984
Author(s):  
Diksha Mishra ◽  
Anand Kumar Singh ◽  
Prateek Singh ◽  
Prachi Pattnaik ◽  
Bajrang Kumar ◽  
...  

2021 ◽  
Vol 20 (6) ◽  
pp. 117-119
Author(s):  
Yusuf Savsatli ◽  
Arzu Karatas

Grafting method in vegetables has been used in common due to positive effects in agriculture. In this context, it is focused that the studies aimed at increasing yield and quality through grafting. This research was carried out to determine the effects of grafting bitter gourd (Momordica charantia L.) onto pumpkin (Cucurbita maxima). Effects of grafting on extract yield, dry matter ratio, ash, and macro and micro elemental content of the fruits and leaves of bitter gourd were determined in greenhouse conditions. Grafting significantly increased the dry matter ratio and extract yield in the bitter gourd fruits and the ash content in young, mature and old leaves. In the fruits, a positive correlation (R = 0.9264) was found only between extract yield and dry matter ratio. The main important effect of grafting in terms of macro and micro elements appeared on the fruits of bitter gourd. The accumulation of Ca2+ in the leaves increased during maturation. The positive effect of grafting in terms of Ca2+ increase in fruits was higher in unripe fruits compared to ripe fruits. In addition to obtaining more extracts from the fruits bitter gourd, the enrichment of its fruits in terms of Ca2+ and K+ and its leaves in terms of Ca2+ increases the importance of these parts in terms of human nutrition.


2021 ◽  
Vol 19 (2) ◽  
pp. 1-9
Author(s):  
Juventine Boaz Odoi ◽  
David Mugeni ◽  
Robert Kiiza ◽  
Betty Apolot ◽  
Samson Gwali

Seed germination and seedling growth performance of Maesopsis eminii and Terminalia catappa under different water soaking treatments were evaluated for 120 days under nursery conditions. A total of 1400 seeds were pre-treated with hot (95oC) and cold water (ambient temperature) by soaking for 12, 24 and 48 hours with a control of no soaking. The seeds were sown directly into polythene pots filled with uniform growth medium (top forest soil, sand and clay soilmixed in a ratio of 5:3:2) to avoid disturbance of the root system after germination. The seeds were sown in a randomized block design with seven treatments and three replicates. Data were analysed using ANOVA in GenStat v18. Results indicated that soaking enhanced seed germination. Soaking of seeds in cold water for 12 hours resulted into higher germination (90% for Terminalia catappa and 85% for Maesopsis eminii) than the control (48%). Soaking period and water temperature significantly influenced seedling vigour (F value = 0.962; p = 0.038). Soaking seeds in cold water for 24 hours enhanced Maesopsis eminii seedling growth by 8.0 cm Terminalia catappa seedlings by 7.4 cm. Seed dormancy, germination percentage and growth performance in hard coated seeds such as Maesopsis eminii and Terminalia catappa can be broken by soaking in cold water for 12-24 hours. Pre-germination treatments significantly influences the germination and seedling growth. Keywords: Maesopsis eminii, Terminalia catappa, seeds, pre-germination, soaking.


2021 ◽  
Vol 17 (2) ◽  
pp. 509-514
Author(s):  
A. Krishna ◽  
Jitendra Kumar S. Hilli

Soapnut (Sapinduas emarginatus) is one of the most primitive precious useful plants since ancient times. This plant was domesticated due to its multifarious usefulness. The study was conducted in College of Forestry, Sirsi during 2019-20 to know the influence of post scarification storage treatments on seed germination and quality in Sapinduas emarginatus. The seeds were collected from in and around Sirsi area. In soap nut, the basic problem is poor seed germination due to hard seed coat. The seeds were treated with concentrated H2So4 for 12min and stored under laboratory condition for six months. At monthly interval, seed samples were drawn and evaluated for post scarification germination. The germination behaviour remarkably influenced due to scarification and without scarification during experimentation. The untreated seeds recorded the maximum germination (68%) compared to scarified seeds (22.85%).Speed of germination was highest in seed without scarification treatment (2.7) compared to scarified seeds. Mean daily germination was highest in without scarified seeds (1.04) and lowest in the scarified seeds (0.32). Peak value was maximum in without scarified seeds (0.50) compared to scarified seeds (0.22). Seedling vigour index was significantly highest in without scarified seeds (1290) compared to scarified seeds (455). In general, scarified seeds with conc. H2SO4 for 12 min affected seed germination due high conc. H2SO4 because of chemical residues remaining in seed that affects the embryo. As the advancement in storage period the seed coat may lose the integrity so, germination was improved in without scarified seeds.


Author(s):  
Ruqiang Tong ◽  
Xinyuan Liu ◽  
Bifan Mu ◽  
Junfeng Wang ◽  
Mengxing Liu ◽  
...  

Background: Understanding the drought tolerance of white clover seeds during germination and early seedling growth and how that is linked to seed maturation in legume species, is crucial to developing new stand establishments.Methods: An experiment was conducted in Randomized Block design in two factorial concept to explore how seed maturation and drought tolerance influence seed germination and early seedling growth using the two factors: seed coat color (yellow and brown, two levels as one factor) and drought intensity (control: 0 MPa [distilled water], mild drought: -0.2 MPa, moderate drought: -0.4 MPa and severe drought: -0.6 MPa, four levels as another factor).Result: In contrast to the yellow-coated seeds, the mean germination percentage, germination index, seedling vigour index and radicle length of the brown-coated seeds were lowered by 7.9%, 23.1%, 20.5% and 12.9%, respectively. Compared to the control, introducing drought conditions reduced the mean of germination percentage, germination index, seedling vigour index and radicle length of yellow-coated seeds by 52.4%, 64.1%, 41.9% and 23.2%, respectively. Meanwhile, drought affected brown-coated seeds more than yellow-coated seeds. The results indicating that the seed quality of white clover seed lots with yellow-colored coats higher than the seed lots with brown-coloured coats. In agronomic practice, a larger proportion of yellow-coated seeds should be harvested to improve new stands successful establishment under environment easy to dry.


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