СIRCULATING SECRETORY IMMUNOGLOBULINA IN SEPTIC DISORDERS

Insufficiency of local immunity can play an important role in pathogenesis of sepsis, including septic (acute) infectious endocarditis (IE). The paper presents data on secretory immunoglobulin A (sIgA) contents in blood serum of patients with sepsis (26 women and 32 men), acute (11 women and 23 men) and subacute (7 women and 13 men) IE, depending on localization of the infection site (angiogenic or non-angiogenic), outcome of the disease and carriage of glutathione-S-transferase P1 gene variants (GSTP1Ile105Val). A control group consisted of 25 women and 24 men without hypertension and ischemic heart disease and lacking evidence of focal and systemic infection, was examined. Laboratory studies were performed with еnzyme immunoassay and allele-specific polymerase chain reaction. We have found that, despite large individual variability of serum sIgA concentration in sepsis and infectious endocarditis, the majority of patients had a significant (on average, 4-fold) IgA increase against controls, in both men and women, especially in acute IE (a mean of 5-fold over control values). Subacute infectious endocarditis is associated with lesser sIgA in circulation than acute IE and sepsis, which may be used for early differential diagnosis of these conditions. There were no gender differences in sIgA contents. In sepsis with non-angiogenic source of infection, the sIgA levels were higher than in angiogenic infection. There was no association of sIgA level with survival (mortality), which excludes this index from predictive markers in sepsis and IE. Carriage of heterozygous GSTP1Ile105Val genotype increases the risk of sepsis and IE development, regardless of clinical course, and homozygous genotype GSTP1Ile105Ile is associated with higher contents of circulating immunoglobulin than in carriers of GSTP1Val105Val genotype. Thus, a wide range of individual variability in of circulating sIgA levels in patients with sepsis and infective endocarditis may be connected with location of infection source and genetic factors.

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The effect of probiotics on the level of immunoglobulin A in women with violation of the biocenosis of the vagina

HEALTH OF WOMAN ◽

10.15574/hw.2017.117.85 ◽

2017 ◽

pp. 85-88

Author(s):

O.I. Ostapenko ◽

◽

V.P. Kvashenko ◽

I.K. Akimova ◽

I.N. Nosova ◽

...

Keyword(s):

Immunoglobulin A ◽

Lactobacillus Rhamnosus ◽

Test System ◽

Main Group ◽

Reproductive Age ◽

Local Action ◽

Serum Immunoglobulin ◽

Control Group ◽

Local Immunity ◽

Serum Iga

The objective: the study of immunomodulatory effects of a probiotic, which contains lyophilized Lactobacillus (Lactobacillus rhamnosus) – 13 mg (2,0ґ109 CFU) and lyophilized bifidobacteria (Bifidobacterium lactis) – 4 mg (2,0ґ109 CFU) the level of serum immunoglobulin IgA as a marker of local immunity in the plasma of women of reproductive age with the violation of the biocenosis of the vagina. Patients and methods. The study involved 86 patients of reproductive age with the violation of the vaginal biocenosis, which were divided into two groups according to received treatment. A survey was conducted for all patients in both groups: determine the level of serum IgA, measuring pH of vaginal environment and the quantification of lactobacilli and pathogenic flora with the help of test-system «Florotsenoz» before treatment and in 6 weeks after treatment. The state of vaginal microbiocenosis in both groups before treatment was homogeneous. Patients in both groups as therapy at the first stage of treatment received, if necessary antimicrobial therapy depending on the selected flora. In the second stage (restoration of microflora) patient of the main group received systemic probiotic combined with a complex prebiotic local action, patients in the control group, the probiotic localy in the form of the vaginal candles or tablets. Results. The research stated the increasing level of serum IgA in blood plasma of patients of the main group compared to control group at 20%, normalizing the pH of the vaginal environment in the main group in 94% of cases, which indicates an increase of immunity in mucosal. Conclusion. The inclusion of the systemic probiotic in the scheme of treatment of disorders of biocenosis of the vagina system enhances the increasing of immunity of the mucous membranes, and the vaginal tablets prebiotic of local action restores the own normal microflora of the vagina. Key words: serum immunoglobulin A, local immunity, vaginal dysbiosis, probiotics, prebiotics, vaginal microbiocenosis, the pH of the vaginal environment.

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LOCAL IMMUNE STATUS AND ETIOLOGICAL FACTORS IN PARATONSILLITIS

Folia Otorhinolaryngologiae et Pathologiae Respiratoriae ◽

10.33848/foliorl23103825-2019-25-3-78-86 ◽

2019 ◽

Vol 25 (3) ◽

pp. 78-86

Author(s):

T.V. Zolotova ◽

◽

A.G. Volkov ◽

P.A. Kondrashov ◽

◽

...

Keyword(s):

Inflammatory Diseases ◽

Immunoglobulin A ◽

Secretory Iga ◽

Control Group ◽

Local Immunity ◽

Immunomodulatory Agents ◽

Etiological Factors ◽

Streptolysin O ◽

Bacterial Lysates ◽

Antigenic Load

Paratonsillitis is one of the most common purulent inflammatory diseases, considered as a manifestation of chronic tonsillitis. The aim of the work is to study the state of local immunity by the level of immunoglobulin A in saliva in patients with paratonsillitis and its changes in the treatment process, to determine the level of antistreptolysin-O in serum, as well as to study the etiological factors of paratonsillites in the microbiological assessment of the species composition of flora in smears from the cavity of paratonsillar abscess. Under our observation there were 152: 32 healthy people and 120 patients with paratonsillitis under abecedarian or abscess stage. It was found that in patients with paratonsillitis there is a significant (p<0.001) decrease in the level of secretory immunoglobulin A in saliva, which indicates a violation of local immunity and requires correction. The level of antibodies to streptolysin-O in the serum of patients with paratonsillitis was significantly - 10.25 times higher than in healthy individuals in the control group, which confirms the high streptococcal antigenic load. The results of microbiological examination of smears from the abscess cavity indicated the release of the most frequent pathogens of paratonsillites - Streptococcus β-haemolythicus, Streptococcus Pneumoniae and Staphylococcus Aureus, with bacterial associations prevailing (63.5%). After a course of antimicrobial therapy, supplemented with immunomodulatory agents in the form of bacterial lysates, there was an increase in the level of secretory IgA in saliva in 10-30 days in 3.3-4.5 times. At the same time, the level of antistreptolysin-O significantly decreased. In patients receiving immunomodulatory therapy in the form of bacterial lysates in the complex treatment of paratonsillitis, for the period of observation of 6 months, there was a decrease in the frequency of relapses of the disease by 2 times.

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EVALUATION OF LOCAL IMMUNITY OF MORBIDITY OF THE MUTUAL IN THE TRADITIONAL THERAPY OF INFLAMMATORY DISEASES OF THE PARODONT

The actual problems in dentistry ◽

10.18481/2077-7566-2018-14-3-11-16 ◽

2018 ◽

Vol 14 (3) ◽

pp. 11-16 ◽

Cited By ~ 1

Author(s):

Надежда Васильева ◽

Nadezhda Vasil'eva ◽

Альбина Булгакова ◽

Al'bina Bulgakova ◽

Эльмира Имельбаева ◽

...

Keyword(s):

Inflammatory Diseases ◽

Periodontal Diseases ◽

Effective Means ◽

Immunoglobulin A ◽

Control Group ◽

Total Protein Content ◽

Total Production ◽

Local Immunity ◽

Traditional Therapy ◽

Mixed Saliva

Subject. The state of local immunity before and with the use of traditional therapy is considered. As a material for assessing local immunity, the quantitative and qualitative characteristics of mixed saliva and the migration test of leukocytes were used, reflecting the severity of inflammatory phenomena in the periodontium and to determine the effectiveness of treatment. The purpose is an evaluation of local immunity of the oral cavity in the traditional therapy of inflammatory periodontal diseases. Methodology. Local immunity was studied in 204 patients with periodontal inflammatory diseases according to the test of leukocyte migration, quantitative and qualitative content of spontaneously secreted mixed saliva: the total protein content, secretory immunoglobulin A (sIgA), lysozyme. All patients underwent traditional therapy. Results. The study revealed an imbalance of local immunity and was characterized by a decrease in total production of mixed saliva in all groups of CDW patients in comparison with the control group. The level of protein was reduced in the group of moderate hippocampus, sIgA in mixed saliva in all patients, especially in cases of severe HCV. The amount of lysozyme is increased in patients with gingivitis, in mild to moderate HGP it corresponds to the content of the control group, it is decreased in patients with moderate and severe periodontitis. In the course of traditional treatment, all patients showed a tendency to normalization of these parameters and was characterized by preservation of reduced production of mixed saliva in all CDW patients, increased lysozyme content and sIgA. However, the restoration of sIgA level in CDW patients to the control group level was not observed. In the group of severe hCG, the level of lysozyme was lower than in control. Conclusions. The use of traditional therapy indicates the preservation of the intensity of immune response in patients with CDW and requires further research and more effective means of action on inflammatory mediators.

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Effectiveness of the use of sanifying set «SanStim» for disinfection eggs before incubation

Scientific Messenger of LNU of Veterinary Medicine and Biotechnology ◽

10.15421/nvlvet8344 ◽

2018 ◽

Vol 20 (83) ◽

pp. 226-229

Author(s):

T.I. Fotina ◽

H.A. Fotina ◽

I. V. Kovalenko

Keyword(s):

Growth And Development ◽

Bacterial Infections ◽

Guanidine Hydrochloride ◽

Experimental Studies ◽

Natural Resistance ◽

Control Group ◽

Bactericidal Action ◽

Source Of Infection ◽

Wide Range ◽

Corrosive Activity

The most vulnerable place in poultry farms is incubation, microorganisms are able to survive the entire period of incubation and penetrate through the eggshell, to be the source of infection with embryos, to affect the reduction of the percentage of conditional young birds, growth and development in the early days of cultivation. The accumulated pathogenic microflora negatively affects the state of the immune system of the bird. Contaminated with conditionally pathogenic and pathogenic microflora egg is the main link in the transmission of all bacterial infections. In Ukraine, for the pre-incubation treatment of eggs, sodium hydroxide, formalin, chloramine, persicum C. are used. These agents exhibit bactericidal properties, but are toxic, have corrosive activity and are carcinogens. The «SunTime» kit consists of polyhexamethylene guanidine hydrochloride and a mixture of organic acid salts (lemon, amber, and apple). These substances provide an effective bactericidal action against a wide range of bacteria, are safe for poultry embryos and personnel, have a stimulating effect on the growth and development of the embryo, provide a prolonged bactericidal action. The purpose of the study of the effectiveness of a complex ecologically safe sanitizers set «SunSim», its application for pre-incubation processing of chicken eggs. In experimental studies, two groups were formed, each with n = 250. In order to disinfect the surface of the shell of the incubation egg, two times aerosol treatment of the experimental group using 0.3% of SunStim was carried out, the first time the sanitation of the incubation eggs was carried out before the laying on the incubation cabinet, the exposure was 40 minutes, the second final treatment was carried out on the 18th day of incubation, to extract, a working solution of organic acids (amber, lemon and apple) to stimulate embryonic viability and increase the natural resistance of the embryos. For the control group, formalin was used. After two times of the sanation of the incubation eggs with the SunStim set, the percentage of decontamination of the shell surface of the incubation eggs was 99.1 ± 0.5%. While analyzing the results of the incubation waste category of «dead and drowning» by 3,6% less compared with the control, and the category of «crippled» was not observed. The use of the SunStim set improves the quality of surface treatment of the shell of incubation eggs, increases the incubation eggs output by 4.5-5.0%, the average body weight of the received young animals in experimental groups exceeds control by 4%.

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Motor Imagery EEG neurofeedback skill acquisition in the context of declarative interference and sleep

10.1101/2020.12.11.420919 ◽

2020 ◽

Author(s):

Mareike Daeglau ◽

Catharina Zich ◽

Julius Welzel ◽

Samira Kristina Saak ◽

Jannik Florian Scheffels ◽

...

Keyword(s):

Individual Differences ◽

Motor Imagery ◽

Skill Acquisition ◽

Brain Injuries ◽

Declarative Memory ◽

Motor Task ◽

Individual Variability ◽

Control Group ◽

Motor Abilities ◽

Wide Range

AbstractMotor imagery (MI) practice in combination with neurofeedback (NF) is a promising supplement to facilitate the acquisition of motor abilities and the recovery of impaired motor abilities following brain injuries. However, the ability to control MI NF is subject to a wide range of inter-individual variability. A substantial number of users experience difficulties in achieving good results, which compromises their chances to benefit from MI NF in a learning or rehabilitation context. It has been suggested that context factors, that is, factors outside the actual motor task, can explain individual differences in motor skill acquisition. Retrospective declarative interference and sleep have already been identified as critical factors for motor execution (ME) and MI based practice. Here, we investigate whether these findings generalize to MI NF practice.Three groups underwent three blocks of MI NF practice each on two subsequent days. In two of the groups, MI NF blocks were followed by either immediate or delayed declarative memory tasks. The control group performed only MI NF and no specific interference tasks. Two of the MI NF blocks were run on the first day of the experiment, the third in the morning of the second day. Significant within-block NF gains in mu and beta frequency event-related desynchronization (ERD) where evident for all groups. However, effects of sleep on MI NF ERD were not found. Data did also not indicate an impact of immediate or delayed declarative interference on MI NF ERD.Our results indicate that effects of sleep and declarative interference context on ME or MI practice cannot unconditionally be generalized to MI NF skill acquisition. The findings are discussed in the context of variable experimental task designs, inter-individual differences, and performance measures.

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Frequency of glutathione S-transferase M1 deletion in smokers with emphysema and lung cancer

Human & Experimental Toxicology ◽

10.1177/096032719701600703 ◽

1997 ◽

Vol 16 (7) ◽

pp. 356-360 ◽

Cited By ~ 75

Author(s):

DJ Harrison ◽

AM Cantlay ◽

F. Rae ◽

D. Lamb ◽

Cad Smith

Keyword(s):

Lung Cancer ◽

Lung Disease ◽

Cigarette Smoke ◽

Lung Tissue ◽

Odds Ratio ◽

Homozygous Deletion ◽

Control Group ◽

Glutathione S Transferase ◽

Gstm1 Gene ◽

Wide Range

1 Genetic variation at the glutathione S-transferase M1 locus (GSTM1) has been associated with a number of apparently unrelated cancers, including lung cancer. Emphysema is a common lung disease often found concomitant with lung cancer. Both emphysema and lung cancer may result from chemical and oxidative damage caused by reactive species present in cigarette smoke or released from neutrophils recruited follow ing cigarette smoke induced injury. GSTM1 may protect against such damage through detoxification of cigarette smoke components. Polymorphism of this gene may thus influence susceptibility not just to lung cancer, but to other forms of lung disease. 2 Resection specimens from a group of 168 lung cancer patients were assessed for the presence of macro scopic centriacinar and panacinar emphysema. DNA was extracted from archival material and genotyped for the GSTM1 polymorphism using the polymerase chain reaction. A control group of 384 anonymous blood donations was used to determine the frequency of the GSTM1 gene deletion in a random control population. Reverse transcription on lung tissue was performed to investigate mRNA expression of GSTM1 and GSTM4. 3 In 57 lung cancer cases with no emphysema there was no association with homozygous deletion of the GSTM1 gene (51% null in cancer and 53% null in control groups). However in 111 patients with emphysema and lung cancer there was an increase in the frequency of deletion (65%, P=0.032) giving an odds ratio of 1.36(0.32-2.40). In 43 cases there was evidence of both centriacinar and panacinar emphy sema. The frequency of GSTM1 deletion was 70% (Odds ratio 2.11, 0.97 - 3.25). Both GSTM1 and GSTM4 mRNAs were expressed in lung tissue. 4 These findings suggest that GSTM1 has a general but rather small protective effect against toxicological injury in the lung which is not specific to cancer. This is of relevance in considering the health effects of exposure to a wide range of reactive chemicals in the environment.

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Results of study of local immunity of the oral cavity in patients with fixed aesthetic dental prostheses and inflammatory periodontal diseases

Kazan medical journal ◽

10.17750/kmj2016-363 ◽

2016 ◽

Vol 97 (3) ◽

pp. 363-367

Author(s):

I R Shafeev ◽

A I Bulgakova ◽

I V Valeev ◽

G Sh Zubairova

Keyword(s):

Oral Cavity ◽

Oral Fluid ◽

Periodontal Diseases ◽

Immunoglobulin A ◽

Main Group ◽

Interleukin 4 ◽

Control Group ◽

Interferon Α ◽

Local Immunity ◽

Dental Prostheses

Aim. To study the state of local immunity of the oral cavity in patients with fixed aesthetic dental prostheses and inflammatory periodontal diseases.Methods. 90 patients with fixed aesthetic dental prostheses and inflammatory periodontal diseases (main group) and 21 patients without dental prostheses and inflammatory periodontal disease (control group) were examined. Immunoglobulin A, sA, G, M, E classes, interleukin-4, -6 and -1β, interferon α contents in oral liquid were determined using enzyme immunoassay.Results.. As a result of our study of immunoglobulins level in saliva in patients of the main and control groups an increase in the immunoglobulin A level, compared with the normal value, statistically significant increase in secretory immunoglobulin A were revealed; difference in the immunoglobulin G level was not determined. The increase in immunoglobulin E level in patients of the main group was determined. When studying the interleukins content in the oral fluid a statistically significant decrease in the concentration of IL-4 and increase in interleukin-6 and -1β content in patients of the main group were determined. The tendency to decrease in the interferon α content in the main group compared to the control group was registered.Conclusion. In patients with fixed aesthetic dental prostheses and inflammatory periodontal diseases humoral immunity imbalance was identified, manifesting in changes of the immunoglobulin classes A, sA and E, interleukin-4, -6 and -1β contents in oral fluid.

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Melatonin protects against cadmium-induced oxidative damage in different tissues of rat: a mechanistic insight

Melatonin Research ◽

10.32794/mr11250018 ◽

2019 ◽

Vol 2 (2) ◽

pp. 1-21 ◽

Cited By ~ 3

Author(s):

Elina Mitra ◽

Bharati Bhattacharjee ◽

Palash Kumar Pal ◽

Arnab Kumar Ghosh ◽

Sanatan Mishra ◽

...

Keyword(s):

Cytochrome C ◽

Oxidative Damage ◽

Protein Carbonyl ◽

Environmental Pollutant ◽

Glutathione S Transferase ◽

Protein Carbonyl Content ◽

Wide Range ◽

Liver And Kidney ◽

Nadh Cytochrome ◽

Cd Exposure

Cadmium (Cd) is a notorious environmental pollutant known for its wide range of toxicities to organisms. Thus, the present study is designed to examine whether melatonin, a potent antioxidant, protects against Cd-induced oxidative damage in the heart, liver and kidney of rats. Cd treatment at a dose of 0.44 mg/kg for 15 days caused severe damage in all these organs. These included significantly increased activities of SGPT, SGOT, lactate dehydrogenase- 1 and 5 and ALP and levels of total lactate, creatinine, lipid peroxidation, protein carbonyl content and reduced glutathione while the activities of superoxide dismutases, catalase, glutathione peroxidase, glutathione reductase and glutathione-S-transferase along with mitochondrial pyruvate dehydrogenase, isocitrate dehydrogenase, α-keto glutarate dehydrogenase, succinate dehydrogenase, NADH-cytochrome-c-oxidoreductase and cytochrome-c-oxidase were significantly reduced by Cd. However, if melatonin was given orally 30 min before Cd injection, all these alterations induced by Cd were significantly preserved by melatonin. Histological observations also demonstrated that Cd exposure caused cellular lesions, promoting necrotic or apoptotic changes. Notably, all these changes were significantly protected by melatonin. The results suggest that melatonin is a beneficial molecule to ameliorate Cd-induced oxidative damage in the heart, liver and kidney tissues of rats with its powerful antioxidant capacity, heavy metal chelating activity and competition of binding sites with Cd to the GSH and catalase.

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Chemoprotective Effects of Propolis on Aflatoxin B1-Induced Hepatotoxicity in Rats: Oxidative Damage and Hepatotoxicity by Modulating TP53, Oxidative Stress

Current Proteomics ◽

10.2174/1570164617666190925121720 ◽

2020 ◽

Vol 17 (3) ◽

pp. 191-199

Author(s):

Seval Yilmaz ◽

Fatih Mehmet Kandemir ◽

Emre Kaya ◽

Mustafa Ozkaraca

Keyword(s):

Oxidative Stress ◽

Gene Expression ◽

Oxidative Damage ◽

Aflatoxin B1 ◽

Tp53 Gene ◽

Control Group ◽

Glutathione S Transferase ◽

Gene Expressions ◽

Cat Gene ◽

Gst Activity

Objective: This study aimed to detect hepatic oxidative damage caused by aflatoxin B1 (AFB1), as well as to examine how propolis protects against hepatotoxic effects of AFB1. Method: Rats were split into four groups as control group, AFB1 group, propolis group, AFB1+ propolis group. Results: There was significant increase in malondialdehyde (MDA) level and tumor suppressor protein (TP53) gene expression, Glutathione (GSH) level, Catalase (CAT) activity, CAT gene expression decreased in AFB1 group in blood. MDA level and Glutathione-S-Transferase (GST) activity, GST and TP53 gene expressions increased in AFB1 group, whereas GSH level and CAT activity alongside CAT gene expression decreased in liver. AFB1+propolis group showed significant decrease in MDA level, GST activity, TP53 and GST gene expressions, GSH level and CAT activity and CAT gene expression increased in liver compared to AFB1 group. Conclusion: These results suggest that propolis may potentially be natural agent that prevents AFB1- induced oxidative stress and hepatotoxicity.

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Physical Activity and Sport for Acquired Brain Injury (PASABI): A Non-Randomized Controlled Trial

Medicina ◽

10.3390/medicina57020122 ◽

2021 ◽

Vol 57 (2) ◽

pp. 122

Author(s):

Marta Pérez-Rodríguez ◽

Saleky García-Gómez ◽

Javier Coterón ◽

Juan José García-Hernández ◽

Javier Pérez-Tejero

Keyword(s):

Quality Of Life ◽

Physical Activity ◽

Brain Injury ◽

Functional Capacity ◽

Intervention Group ◽

Chronic Phase ◽

Acquired Brain Injury ◽

Control Group ◽

Wide Range

Background and objectives: Acquired brain injury (ABI) is the first cause of disability and physical activity (PA) is a key element in functional recovery and health-related quality of life (HRQoL) during the subacute and chronic phases. However, it is necessary to develop PA programs that respond to the heterogeneity and needs of this population. The aim of this study was to assess the effectiveness of a PA program on the HRQoL in this population. Materials and Methods: With regard to recruitment, after baseline evaluations, participants were assigned to either the intervention group (IG, n = 38) or the control group (CG, n = 35). Functional capacity, mood, quality of life and depression were measured pre- and post-intervention. The IG underwent the “Physical Activity and Sport for Acquired Brain Injury” (PASABI) program, which was designed to improve HRQoL (1-h sessions, two to four sessions/week for 18 weeks). The CG underwent a standard rehabilitation program without PA. Results: Results for the IG indicated significant differences and large effect sizes for the physical and mental dimensions of quality of life, as well as mood and functional capacity, indicating an increase in HRQoL. No significant differences were found for the CG across any variables. Conclusions: The PASABI program was feasible and beneficial for improving physiological and functionality variables in the IG. The wide range of the activities of the PASABI program allow its application to a large number of people with ABI, promoting health through PA, especially in the chronic phase.

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