scholarly journals The effect of titanium dioxide nanoparticles on the relative expression of catalase, P450, SOD, diTDS and WRKY genes of Vitex agnus-castus L.

2021 ◽  
Vol 49 (4) ◽  
pp. 12292
Author(s):  
Seyed M. MOSHIRIAN FARAHI ◽  
Alireza IRANBAKHSH ◽  
Homa MAHMOODZADEH ◽  
Mostafa EBADI
Keyword(s):  
Titanium Dioxide ◽  
Tio2 Nanoparticles ◽  
Biosynthetic Pathway ◽  
Titanium Dioxide Nanoparticles ◽  
Leaf Tissue ◽  
Relative Expression ◽  
Qrt Pcr ◽  
Plant Cultivation ◽  
Expression Of Genes ◽  
Agnus Castus

Each environmental factor is able to change the way genes are expressed. Application of nanoparticles also affects the expression of different genes in plants. The aim of this study was to investigate the effect of three different concentration of titanium dioxide nanoparticles, TiO2 (zero, 200 and 800 micrograms per milliliter) on the relative expression of catalase, P450, SOD, diTDS and WRKY genes in Vitex plant leaf tissue using qRT- PCR. Plant cultivation was carried out in 2018 in the greenhouse of Islamic Azad University of Mashhad. The experiment was arranged as completely random design with 5 replications. XRD measurements showed that applied TiO2 nanoparticles were in the form of anatase. Statistical analysis of gene expression in treated leaves of Vitex plant with TiO2 nanoparticles showed that this nanoparticle significantly affected the expression of catalase, P450, SOD, diTPS and WRKY genes. A concentration of 800 micrograms per milliliter of TiO2 nanoparticle increased the expression of catalase, P450, SOD and WRKY genes and decreased the expression of diTPS gene. In contrast, concentrations of 200 micrograms per milliliter only increased the expression of catalase and WRKY genes. The expression of the diTPS gene under treatments of 200 and 800 micrograms per liter of TiO2, compared with control, decreased by 2.1 and 0.46, respectively. Overall, the nanoparticle was able to influence the expression of genes in the biosynthetic pathway of terpenoids, as well as the plant's antioxidant enzymes, depending on the concentration of nanoparticles.

Dispersion and stability of titanium dioxide nanoparticles in aqueous suspension: effects of ultrasonication and concentration

2013 ◽  
Vol 67 (1) ◽  
pp. 147-151 ◽  
Author(s):  
J. Qi ◽  
Y. Y. Ye ◽  
J. J. Wu ◽  
H. T. Wang ◽  
F. T. Li
Keyword(s):  
Titanium Dioxide ◽  
Zeta Potential ◽  
Tio2 Nanoparticles ◽  
Titanium Dioxide Nanoparticles ◽  
Aqueous Suspension ◽  
Solution Chemistry ◽  
Hydrodynamic Diameter ◽  
Nanoparticles Dispersion ◽  
Nanoparticles Concentration ◽  
Nanoparticles Suspension

The increasing applications of titanium dioxide (TiO2) nanoparticles raise concerns about their potential environmental impacts. To investigate the fate and transport of TiO2 nanoparticles in aqueous suspension, ultrasonication is widely used for the dispersion of TiO2 nanoparticles in laboratory-scale studies. There is a pressing need for detailed information on the dispersion and stability of TiO2 nanoparticles. This study investigated the change of size, zeta potential, and pH of TiO2 nanoparticles aqueous suspension under different conditions of ultrasonication and concentrations. It was found that the hydrodynamic diameter of TiO2 nanoparticles decreased with increasing suspension concentration and remained stable for more than 1 hour after sonication, which is enough for experimental research. The pH decreased with increasing nanoparticles concentration. Ultrasonication remarkably improved zeta potential to be above 15 mV for all the samples. Therefore, 20 minutes of ultrasonication (180 W) is sufficient for the dispersion of this rutile TiO2 nanoparticles suspension, which can remain stable for more than 1 hour. However, the optimum sonication time for TiO2 nanoparticles dispersion is influenced by many factors, such as TiO2 nanoparticles concentration, solution chemistry, and sonicator parameters.

scholarly journals Facile Green Synthesis and Characterization of Titanium Dioxide Nanoparticles Using Kigelia africana (Lam) Benth., Aqueous Leaf Extract and its Antioxidant and Antibacterial Activity

2022 ◽  
Vol 34 (2) ◽  
pp. 409-414
Author(s):  
N. Usha Rani ◽  
P. Pavani ◽  
P.T.S.R.K. Prasad Rao
Keyword(s):  
Titanium Dioxide ◽  
Green Synthesis ◽  
Tio2 Nanoparticles ◽  
Leaf Extract ◽  
Titanium Dioxide Nanoparticles ◽  
Average Particle Size ◽  
Radical Scavenging ◽  
Average Particle ◽  
Absorption Bands ◽  
Aqueous Leaf Extract

Titanium nanoparticles are toxic to bacteria and have a widespread applications in different fields of research. Hence the present study aimed to synthesize the titanium dioxide nanoparticles by adopting green synthesis methodology using Kigelia africana leave extract as a biological reducing agent. The UV absorption spectra show characteristic absorption maxima corresponding to TiO2 nanoparticles at a wavelength of 512 nm confirms the formation of nanosized tin particles. The FT-IR spectrum of TiO2 nanoparticles show absorption bands at 3609 cm-1 and 3227 cm-1 corresponding to O-H stretching in alcoholic and carboxylic compounds, respectively. Absorption peaks at 1607, 2834, 1654 and 1324 cm-1 correspond to aromatic C=C vibrations, C-H stretching in aldehydes, C-H bending vibrations and aromatic C-N stretching vibrations, respectively. This confirms the involvement of bioactive compounds from the plant extract. The SEM and EDX studies confirmed that the nanoparticles are spherical to oval shape with an average particle size of 46 nm. The metal content in the nanoparticles was found to be 58.71%. The synthesized nanoparticles have potential growth inhibition activity against Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli). The DPPH radical scavenging activity of the nanoparticles synthesized was compared with that of aqueous leaf extract and standard ascorbic acid and proved that the nanoparticles have enhanced activity than aqueous leaf extract. The IC50 of the leaf extract, nanoparticles and the standard was found to be 31.55, 75.82 and 84.95 μg/mL, respectively. Kigelia africana leaf is shown in this work to be a valuable bioagent in the biosynthesis of TiO2 nanoparticles with increased biological activity.

scholarly journals Phytosynthesis of Titanium Dioxide Nanoparticles Using King of Bitter Andrographis paniculata and Its Embryonic Toxicology Evaluation and Biomedical Potential

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
S. Rajeshkumar ◽  
J. Santhoshkumar ◽  
Leta Tesfaye Jule ◽  
Krishnaraj Ramaswamy
Keyword(s):  
Titanium Dioxide ◽  
Green Synthesis ◽  
Tio2 Nanoparticles ◽  
Titanium Dioxide Nanoparticles ◽  
Spherical Shape ◽  
Andrographis Paniculata ◽  
Potential Hazard ◽  
Average Size ◽  
Herbal Plant ◽  
Biomedical Potential

Phytosynthesis particles are the efficient activity of biomedical and environmental. In this present study, the green synthesis of titanium dioxide (TiO2) nanoparticles using the king of bitter herbal plant Andrographis paniculata was synthesized and characterized using XRD, SEM, HRTEM, AFM, and antimicrobial, antioxidant, and antidiabetic activities. The size of the particles HRTEM shows 50 nm, and SEM shows the spherical shape, which reveals the synthesis of TiO2 nanoparticles. XRD spectrum shows crystallinity of nanoparticles, and an average size is calculated about 22.97 nm. The phytosynthesis TiO2 shows the antioxidant and antidiabetic activities. Similarly, toxicity studies have demonstrated the hatching and viability LD 50 value of TiO2 250 μg/L. The current study’s findings suggested that phytosynthesis TiO2 using extract of Andrographis paniculata exposure to potential hazard factors to biomedical and environmental uses.

scholarly journals Ion-Trap Mass Spectrometric Analysis of Bisphenol A Interactions With Titanium Dioxide Nanoparticles and Milk Proteins

Molecules ◽  
2020 ◽  
Vol 25 (3) ◽  
pp. 708
Author(s):  
Edward P.C. Lai ◽  
Hendrik Kersten ◽  
Thorsten Benter
Keyword(s):  
Titanium Dioxide ◽  
Quantitative Analysis ◽  
Bisphenol A ◽  
Tio2 Nanoparticles ◽  
Ion Trap ◽  
Titanium Dioxide Nanoparticles ◽  
Milk Proteins ◽  
Sodium Formate ◽  
Spectrometric Analysis ◽  
Milk Whey

Quantitative analysis of endocrine-disrupting molecules such as bisphenol A (BPA) in freshwater to determine their widespread occurrence in environmental resources has been challenged by various adsorption and desorption processes. In this work, ion trap mass spectrometry (ITMS) analysis of BPA was aimed at studying its molecular interactions with titanium dioxide (TiO2) nanoparticles and milk whey proteins. Addition of sodium formate prevented TiO2 nanoparticles from sedimentation while enhancing the electrospray ionization (ESI) efficiency to produce an abundance of [BPA + Na]+ ions at m/z 251.0. More importantly, the ESI-ITMS instrument could operate properly during a direct infusion of nanoparticles up to 500 μg/mL without clogging the intake capillary. Milk protein adsorption of BPA could decrease the [BPA + Na]+ peak intensity significantly unless the proteins were partially removed by curdling to produce whey, which allowed BPA desorption during ESI for quantitative analysis by ITMS.

Titanium Dioxide Nanoparticles and Cetylpyridinium Chloride Enriched Glass-Ionomer Restorative Cement: A Comparative Study Assessing Compressive Strength and Antibacterial Activity

2019 ◽  
Vol 43 (1) ◽  
pp. 42-45
Author(s):  
Nida Hamid ◽  
Ravishankar Lingesha Telgi ◽  
Amit Tirth ◽  
Vaibhav Tandon ◽  
Smita Chandra ◽  
...  
Keyword(s):  
Compressive Strength ◽  
Antibacterial Activity ◽  
Titanium Dioxide ◽  
Tio2 Nanoparticles ◽  
Titanium Dioxide Nanoparticles ◽  
Cetylpyridinium Chloride ◽  
Testing Machine ◽  
Glass Ionomer Cement ◽  
Glass Ionomer ◽  
Significant Difference

Objective: To evaluate the addition of titanium dioxide (TiO2) nanoparticles and cetylpyridinium chloride (CPC) on the compressive strength and antibacterial activity of conventional glass-ionomer cement (GIC). Study design: TiO2 nanoparticles enriched GIC was prepared by adding 3% TiO2 nanoparticles (w/w) into the powder component of conventional GIC. CPC containing GIC was developed by incorporating 1% CPC (w/w) into conventional GIC powder. Samples were segregated into three groups: GIC with 3% TiO2 nanoparticles, GIC with 1% CPC and unmodified conventional GIC. Compressive strength was assessed using the universal testing machine on cylindrical specimens made from each material. Antibacterial activity was assessed by measuring inhibition zones on Mitis Salivarius Bacitracin (MSB) agar inoculated with pure strain of Streptococcus mutans (S. mutans). Results: GIC containing TiO2 nanoparticles exhibited significantly greater compressive strength as compared with CPC and conventional GIC groups (P < 0.01). However, there was no significant difference between the compressive strengths of CPC and conventional GIC group (P >0.05). Antibacterial activity was significantly greater for TiO2 group than conventional GIC (P <0.05). CPC increased the antibacterial activity of conventional GIC, though not significantly. Conclusion: The addition of 3% TiO2 nanoparticles improves the compressive strength of GIC as well as its antibacterial activity against S. mutans.

scholarly journals Appraisal of Comparative Therapeutic Potential of Undoped and Nitrogen-Doped Titanium Dioxide Nanoparticles

Molecules ◽  
2019 ◽  
Vol 24 (21) ◽  
pp. 3916 ◽  
Author(s):  
Muhammad Arslan Ahmad ◽  
Yang Yuesuo ◽  
Qiang Ao ◽  
Muhammad Adeel ◽  
Zhang Yan Hui ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Titanium Dioxide ◽  
Protein Kinase ◽  
Tio2 Nanoparticles ◽  
Therapeutic Potential ◽  
Titanium Dioxide Nanoparticles ◽  
Doped Tio2 ◽  
X Ray ◽  
Nitrogen Doped ◽  
Protein Kinase Inhibition

Nitrogen-doped and undoped titanium dioxide nanoparticles were successfully fabricated by simple chemical method and characterized using x-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive x-ray (EDX), and transmission electron microscopy (TEM) techniques. The reduction in crystalline size of TiO2 nanoparticles (from 20–25 nm to 10–15 nm) was observed by TEM after doping with N. Antibacterial, antifungal, antioxidant, antidiabetic, protein kinase inhibition and cytotoxic properties were assessed in vitro to compare the therapeutic potential of both kinds of TiO2 nanoparticles. All biological activities depicted significant enhancement as a result of addition of N as doping agent to TiO2 nanoparticles. Klebsiella pneumoniae has been illuminated to be the most susceptible bacterial strain out of various Gram-positive and Gram-negative isolates of bacteria used in this study. Good fungicidal activity has been revealed against Aspergillus flavus. 38.2% of antidiabetic activity and 80% of cytotoxicity has been elucidated by N-doped TiO2 nanoparticles towards alpha-amylase enzyme and Artemia salina (brine shrimps), respectively. Moreover, notable protein kinase inhibition against Streptomyces and antioxidant effect including reducing power and % inhibition of DPPH has been demonstrated. This investigation unveils the more effective nature of N-doped TiO2 nanoparticles in comparison to undoped TiO2 nanoparticles indicated by various biological tests. Hence, N-doped TiO2 nanoparticles have more potential to be employed in biomedicine for the cure of numerous infections.

A novel paper based colorimetric assay for the detection of TiO2 nanoparticles

2018 ◽  
Vol 10 (3) ◽  
pp. 275-280 ◽  
Author(s):  
Gonca Bulbul ◽  
Hamed Eskandarloo ◽  
Alireza Abbaspourrad
Keyword(s):  
Methylene Blue ◽  
Titanium Dioxide ◽  
Tio2 Nanoparticles ◽  
Colorimetric Assay ◽  
Titanium Dioxide Nanoparticles

In this communication, we propose a new paper-based platform functionalized with methylene blue (MB) for the detection and removal of titanium dioxide nanoparticles (TiO2 NPs).

Titanium Dioxide Nanoparticles Induced Cytotoxicity in Rat Retinal Ganglion Cells under Ultraviolet B Irradiation

2012 ◽  
Vol 531-532 ◽  
pp. 500-503
Author(s):  
Da Dong Guo ◽  
Hong Sheng Bi ◽  
Qiu Xin Wu ◽  
Dao Guang Wang
Keyword(s):  
Titanium Dioxide ◽  
Tio2 Nanoparticles ◽  
Uv Irradiation ◽  
Ganglion Cells ◽  
Titanium Dioxide Nanoparticles ◽  
Ultraviolet B ◽  
Target Cells ◽  
Retinal Ganglion ◽  
Ultraviolet B Irradiation

Titanium dioxide (TiO2) nanoparticles have been widely used in many fields, including biomedicine, cosmetics and environmental engineering. Recently, it was reported that TiO2 nanoparticles could exert genotoxicity on organisms via the generation of reactive oxygen species (ROS). In the present study, we investigated the effect of TiO2 nanoparticles on RGC-5 cells with or without ultraviolet (UV) B irradiation through MTT assay and determination of hydroxyl radical production. The results demonstrated that TiO2 nanoparticles could efficiently inhibit the growth of RGC-5 cells in the presence of UVB irradiation, whereas there was little impact on target cells in the absence of UV irradiation; UV irradiation could apparently increase the level of hydroxyl radicals in RGC-5 cells and further efficiently inhibit the growth of RGC-5 cells, suggesting that UV irradiation plays an important role in growth inhibition in RGC-5 cells exposed to TiO2 nanoparticles.

scholarly journals Finding Nano: Challenges Involved in Monitoring the Presence and Fate of Engineered Titanium Dioxide Nanoparticles in Aquatic Environments

Water ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 734
Author(s):  
Simone Heilgeist ◽  
Ryo Sekine ◽  
Oz Sahin ◽  
Rodney A. Stewart
Keyword(s):  
Titanium Dioxide ◽  
Tio2 Nanoparticles ◽  
Inductively Coupled Plasma ◽  
Titanium Dioxide Nanoparticles ◽  
Aqueous Media ◽  
Experimental Studies ◽  
Aquatic Environments ◽  
Nano Tio2 ◽  
Icp Ms ◽  
Modelling Studies

In recent years, titanium dioxide (TiO2) has increasingly been used as an inorganic ultraviolet (UV) filter for sun protection. However, nano-TiO2 may also pose risks to the health of humans and the environment. Thus, to adequately assess its potential adverse effects, a comprehensive understanding of the behaviour and fate of TiO2 in different environments is crucial. Advances in analytical and modelling methods continue to improve researchers’ ability to quantify and determine the state of nano-TiO2 in various environments. However, due to the complexity of environmental and nanoparticle factors and their interplay, this remains a challenging and poorly resolved feat. This paper aims to provide a focused summary of key particle and environmental characteristics that influence the behaviour and fate of sunscreen-derived TiO2 in swimming pool water and natural aquatic environments and to review the current state-of-the-art of single particle inductively coupled plasma mass spectrometry (SP-ICP-MS) approaches to detect and characterise TiO2 nanoparticles in aqueous media. Furthermore, it critically analyses the capability of existing fate and transport models to predict environmental TiO2 levels. Four particle and environmental key factors that govern the fate and behaviour of TiO2 in aqueous environments are identified. A comparison of SP-ICP-MS studies reveals that it remains challenging to detect and characterise engineered TiO2 nanoparticles in various matrices and highlights the need for the development of new SP-ICP-MS pre-treatment and analysis approaches. This review shows that modelling studies are an essential addition to experimental studies, but they still lack in spatial and temporal resolution and mostly exclude surface transformation processes. Finally, this study identifies the use of Bayesian Network-based models as an underexplored but promising modelling tool to overcome data uncertainties and incorporates interconnected variables.

Sign in / Sign up

Export Citation Format

Share Document