scholarly journals The Investigation of Decontamination Effects of Ozone Gas on Microbial Load and Essential Oil of Several Medicinal Plants

2013 ◽  
Vol 5 (1) ◽  
pp. 34-38 ◽  
Author(s):  
Razieh VALI ASILL ◽  
Majid AZIZI ◽  
Maasome BAHREINI ◽  
Hossein AROUIEE

Today, Ozone as a disinfectant method, without putting on the harmful effects on human and plant products, it is alternative common methods for disinfection of plant material. The research as a factorial experiment was conducted on the basis of randomized complete block design with three replications and the effects of Ozone gas on decreasing the microbial load of some important medicinal plants include: Peppermint (Mentha piperita), Summer savory (Satureja hortensis), Indian valerian(Valeriana wallichii), Meliss (Melissa officinalis) and Iranian thyme (Zataria multiflora) were investigated. Medicinal plants leaves were treated with Ozone gas concentration 0.3, 0.6 and 0.9 ml/L at times of 10 and 30 then total count, coliform and mold and yeast of the samples were studied. The result showed that Ozone gas decreases microbial load of medicinal plants samples. But Ozone gas and Ozone gas in medicinal plants interaction effect had no effect on essential oil content. The lowest and the highest of microbial load were detected in samples treated with concentration of 0.9 ml/L of Ozone gas and control respectively. The highest and the lowest of microbial load were observed in Iranian thyme and Indian valerian respectively. Also result showed that Ozone gas treatment for 30 min had the greatest of effect in reducing the microbial load and 0.9 ml/L Ozone gas concentration had the lowest of microbial load. Results of this survey reflect that the use of Ozone as a method of disinfection for medicinal plants is a decontamination.

2021 ◽  
Vol 20 (2) ◽  
pp. 43-52
Author(s):  
Aliakbar Goudarzian ◽  
Abdollah Ghasemi Pirbalouti ◽  
Mohammadreza Hossaynzadeh

Peppermint (Mentha × piperita L.) is cultivated for its benefits in pharmaceutical, medicinal, and cosmetic industries. The well-known essential oil of Mentha × piperita L. is widely produced and used all over the world. The aim of present study was to evaluate the impacts of different concentrations of chitosan on the quality and quantity of the essential oil from the aerial parts of peppermint under inoculation of the rhizomes of peppermint seedlings with arbuscular mycorrhizal fungi. Experimental treatments were arranged as factorial design in a completed random block design. The highest essential oil yield (2.4 mL 100 g–1 dry matter) was obtained from the peppermint plants under foliar sprayed at 5 g L–1 chitosan along the inoculum with arbuscular mycorrhizal fungi. For evaluation of phytochemical characteristics, the contents of the main constituents of the peppermint essential oils such as menthol, menthone, etc. (oxygenated monoterpenes and monoterpenes hydrocarbons) under different treatments were analyzed by GC-FID and GC/MS. Results indicated that using chitosan foliar meaningfully raised the amount of menthol, as the major constituent and quality index (>60% v/w), in the essential oil from the peppermint plants inoculation with arbuscular mycorrhizal, however, the plants under the foliar spray of chitosan (without inoculum) revealed the highest amounts of menthone and limonene. In conclusion, we found that the foliar-applied chitosan along inoculation with arbuscular mycorrhizal fungi can be improved quantity and quality active substances of Mentha × piperita L. such as the contents of essential oil, menthol, and balance menthol/menthone.


2019 ◽  
Vol 6 (1) ◽  
pp. 51
Author(s):  
Roza Puspita ◽  
Resti Rahayu ◽  
Mairawita Mairawita ◽  
Nasril Nasir ◽  
Nurmansyah Nurmansyah

The toxic effect of essential oil from waste  cinnamon leaf (Cinnamomum burmanii)  against pests of cocoa fruit Helopeltis antonii (Hemiptera; Miridae),  has been done in Green House, Research Station Spice and Medicinal Plants, Laing Solok, West Sumatra in April to May 2016. The experiment aimed to determine repellant and lethal  concentrations of essential oil from waste s cinnamon leaf (C. burmanii) for H. antonii. There were 5  treatments  arranged in Complately Randomized Design with 4 replications. The treatments tested were essentialoil concentration f  (0.05 ml/tube, 0.1 ml/tube, 0.2 ml/tube and 0.5 ml/tube) and control. The results showed that the effective repellent concentration were 0,05 ml/tube and 0,1 ml/tube which repelled  H. antonii  0,03 % . The effecive lethal concentration was  0,05 ml/tube which killed  H. antonii  57,50 %. Concentration used was  highly  toxic which killed l H. Antonii within  six hours so of the repellent nature was notobserved.


2018 ◽  
Vol 10 (2) ◽  
pp. 245-250
Author(s):  
Reza Dehghani BIDGOLI ◽  
Mohammad Javad MAHDAVI

An experiment was conducted during 2015-2016 as a split factorial in a randomized complete block design (RCBD) with three replications. Experimental factors were nitrogen fertilizer at four levels 0, 50, 100 and 150 kg/ha as the main factor and two types of green manure. Alfalfa (Medicago sativa) and secale (Secale montanum) in two states (use and non-use) were considered as sub-factors. Medicago sativa and Secale montanum were cultivated as green manure in September 2015 and returned into the soil by ploughing at the fall of 2015. Thereafter, Mentha piperita was planted and the crop was taken care of in order to collect the samples. The highest increase of peppermint essential oil percentage and essential oil yield in different treatments resulted from the application of 150 kg/ha of pure nitrogen with 0.35% and 45.60 kg/ha, as well as the use of M. sativa with 0.43% and 55.80 kg/ha, the use of S. montanum with 0.44% and 49.50 kg/ha, respectively. The highest essential oil percentage and essential oil yield were recorded under the influence of the double interaction use of 150 kg/ha of pure nitrogen and use of green manure of M. sativa with average 0.62% and 80.30 kg/ha, respectively. The triple interaction of experimental factors on traits tested showed that nitrogen treatment of 150 kg/ha with M. sativa and S. montanum produced the highest percentage of essential oil and essential oil yield of average 0.73% and 91.65 kg/ha, respectively.


Author(s):  
Meryem Yeşil ◽  
Mehmet Muharrem ÖZCAN

This study aimed at investigating the effect of ontogenetic and diurnal variability on yield and quality criteria of Mentha piperita. The research was conducted in the Ordu province of Turkey for two years. The trial was established in a randomised complete block design with 3 replications according to factorial regulations. Four different harvesting periods (budding, first flowering, 50% flowering, 100% flowering) and three harvesting hours (9:00 a.m., 1:00 p.m., 5:00 p.m.) were evaluated. A significant difference was determined between the trial years in all characters examined: plant height 55.9–69.0 cm, fresh herb yield 3 236–16 438 kg/ha, drug-herb yield 1 748–2 862 kg/ha, fresh leaf yield 3 477–4 506 kg/ha, drug leaf yield 968–1 253 kg/ha, essential oil ratio 2.0–2.5% and essential oil yield that varied between 19–28 L/ha. The highest yields were obtained at the beginning of flowering in drug leaf yield and 100% flowering period for all other characteristics. Diurnal variability was statistically significant only in the rate of essential oil, and the highest rate occurred in the 9:00 a.m. harvest.  


2019 ◽  
Vol 3 (01) ◽  
pp. 22-25
Author(s):  
REZA DEHGHANI BIDGOLI

Abstract. Bidgoli RD. 2019. Effect of inoculation of two Aztobacter and nitrogen fertilizer on of peppermint (Mentha piperita) essential oil. Asian J Agric 3: 22-25. This study was conducted to study changes of percentage and yield of essential oil of peppermint (Mentha piperita) as a split factorial in a randomized complete block design (RCBD) with three replications in 2016-2017. The experimental treatments were nitrogen at four levels 0, 50, 100 and 150 kg/ha as the main factor and two strains of PGPR (Aztobacter chroococcum MZ11, MZ26) in two state of (use and non-use) was considered as sub factors. Results showed that use of these two of A. chroococcum strains has led to increase in the percentage and yield of peppermint essential oil more than all Nitrogen fertilizer treatments. Also, the highest peppermint essential oil percentage (0.73%) obtained the in the of triple interaction of Nitrogen (100 kg/ha) A. chroococcum MZ11 and A. chroococcum MZ26 The highest essential oil yield (91.65 kg/ha)observed under the influence of the triple interaction of Nitrogen (100 kg/ha) , A. chroococcum MZ11 and A. chroococcum MZ26.


2016 ◽  
Vol 49 (1) ◽  
pp. 51-65
Author(s):  
F. Abdollahi ◽  
A. Salehi ◽  
R. Shahabi ◽  
A. Rahimi

Abstract Coriander (Coriandrum sativum) is one of the medicinal plants that its essential oil yield has abundance medicinal and food value. One of the main concepts in the production of medicinal plants is to increase of yield by biofertilizers. In order to evaluate the separate and combined effect of nitrogen and biofertilizer Nitroxin (mixture of bacteria Azotobacter and Azospirillum) fertilizer on vegetative traits, grain yield and essential oil yield of coriander, an experiment was conducted as a factorial in a randomized complete block design (RCBD) with three replications in the field research of Islamic Azad University, Jiroft, Iran. The experiment factors were consisted of three levels of nitrogen (0, 75 and 150 kg·ha−1) and three levels of Nitroxin biofertilizer (0, 2 and 4 L·ha−1). The results showed that application of 150 kg·ha−1 N significantly increased height and stem diameter, number of lateral branches and shoot dry weight, compared with control and application of 75 kg·ha−1 N. Nitroxin biofertilizer significantly improved vegetative traits of coriander when compared with control, so that application of 4 L·ha−1 of Nitroxin caused increase the mean of traits compared to control. In the most evaluated growth traits, the highest means were obtained with combining of 150 kg·ha−1 N with 4 L·ha−1 of Nitroxin. Application of 150 kg·ha−1 N increased the number of umbels per plant, 1000 seeds weigh, seed yield and essential oil yield, 29.9, 33.0, 72.9, 40.7, 147.4 and 177.1 percent, compared with the control, respectively. In the presence of Nitroxin, yield and yield component and essential oil yield of coriander increased significantly. Maximum average of these traits obtained when 4 L·ha−1 of Nitroxin was applied. Integrated of nitrogen and Nitroxin increased yield and yield component and essential oil yield significantly, when compare with application separately of them. So that the maximum mean of these traits obtained with combined application of 150 kg·ha−1 nitrogen and 4 L·ha−1 of Nitroxin. The results of this research was showed that application Nitroxin biofertilizer have an important role in increase of quantity and quality yield of coriander medicinal plant and can be used as an alternative of nitrogen fertilizer in sustainable agriculture.


2016 ◽  
Vol 36 (85) ◽  
pp. 71
Author(s):  
Kamila Cardoso Pereira ◽  
Fernanda Rocha Reda ◽  
Graziela Piveta ◽  
Flávio Augusto De Oliveira Garcia

O objetivo foi avaliar o efeito dos óleos essenciais de Mentha piperita, Cymbopogon nardus e Eucalyptus globulus, na qualidade sanitária e fisiológica de sementes e mudas de Schinus molle L. As sementes foram tratadas com os óleos essenciais nas concentrações de 10%, em 1 μL g-1 de sementes, 20% , em 2 μL g-1 de sementes, 30%, em 3 μL g-1 de sementes e o tratamento controle. Após os tratamentos, as sementes foram avaliadas pelo teste de sanidade, utilizando-se o método do papel de filtro (“blotter test”) e pelo teste de germinação, onde as sementes foram colocadas entre substrato vermiculita. Para as contagens, foram consideradas sementes germinadas e sementes mortas. Na avaliação da qualidade das mudas, mediu-se o comprimento da parte aérea, raízes, altura total e diâmetro do colo. A utilização dos óleos essenciais de citronela e menta reduziu a incidência dos diferentes patógenos presentes nas sementes de S. molle. O óleo essencial de menta a 20% e 30 % favoreceu a germinação das sementes. O óleo essencial de eucalipto, em qualquer concentração, aumentou o crescimento das mudas.Evaluation of essential oils in health and physiological quality of Schinus molle seeds and seedlingsThe objective was to evaluate the effect of Mentha piperita, Cymbopogon nardus and Eucalyptus globules essential oils, in sanitary and physiological quality of Schinus molle L. seeds and seedlings. The seeds were treated with essential oils at concentrations of 10%, on 1 μL g-1, 20%, on 2 μL g-1, 30% , on 3 μL g-1 and control. After treatments, seeds were evaluated by sanity test, using filter paper method (“blotter test”) and by germination test, where the seeds were placed between vermiculite substrate. For counts, sprouted and dead seeds were considered. Shoot length, root, total height and stem diameter were measured for seedlings evaluation. The use of citronella and mint essential oils reduced the incidence of different pathogens in S. molle seeds. Mint essential oil at 20% and 30% were effective to promote seeds germination. Eucalyptus essential oil in any concentration was efficient to increase seedlings growth.Index terms: Mentha piperita; Cymbopogon nardus; Eucalyptus globulus 


Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
YY Kamrani ◽  
M Amanlou ◽  
A Yazdanyar ◽  
A AdliMoghaddam ◽  
SN Ebrahimi

Author(s):  
Azadeh Foroughi ◽  
Pouya Pournaghi ◽  
Fariba Najafi ◽  
Akram Zangeneh ◽  
Mohammad Mahdi Zangeneh ◽  
...  

Medicinal plants are considered modern resources for producing agents that could act as alternatives to antibiotics in demeanor of antibiotic-resistant bacteria. The aim of the study was to evaluate the chemical composition and antibacterial activities of essential oil of Foeniculum vulgare (FV) against Pseudomonas aeruginosa and Bacillus subtilis. Gas chromatography mass spectrometry was done to specify chemical composion. As a screen test to detect antibacterial properties of the essential oil, agar disk and agar well diffusion methods were employed. Macrobroth tube test was performed to determinate MIC. The results indicated that the most substance found in FV essential oil was Trans-anethole (47.41 %), also the essential oil of FV with 0.007 g/ml concentration has prevented P. aeruginosa and with 0.002 g/ml concentration has prevented B. subtilis from the growth. Thus, the research represents the antibacterial effects of the medical herb on test P. aeruginosa and B. subtilis. We believe that the article provide support to the antibacterial properties of the essential oil. The results indicate the fact that the essential oil from the plant can be useful as medicinal or preservatives composition.


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