scholarly journals Efek Toksik Minyak Atsiri Limbah Daun Kayu Manis (Cinnamomum burmanii (Nees & T.Nees) Blume.) dalam Mengendalikan Helopeltis antonii Signoret pada Tanaman Kakao secara in Vitro

2019 ◽  
Vol 6 (1) ◽  
pp. 51
Author(s):  
Roza Puspita ◽  
Resti Rahayu ◽  
Mairawita Mairawita ◽  
Nasril Nasir ◽  
Nurmansyah Nurmansyah
Keyword(s):  
Medicinal Plants ◽  
Essential Oil ◽  
Toxic Effect ◽  
Lethal Concentration ◽  
Research Station ◽  
Green House ◽  
Randomized Design ◽  
West Sumatra ◽  
And Control

The toxic effect of essential oil from waste  cinnamon leaf (Cinnamomum burmanii)  against pests of cocoa fruit Helopeltis antonii (Hemiptera; Miridae),  has been done in Green House, Research Station Spice and Medicinal Plants, Laing Solok, West Sumatra in April to May 2016. The experiment aimed to determine repellant and lethal  concentrations of essential oil from waste s cinnamon leaf (C. burmanii) for H. antonii. There were 5  treatments  arranged in Complately Randomized Design with 4 replications. The treatments tested were essentialoil concentration f  (0.05 ml/tube, 0.1 ml/tube, 0.2 ml/tube and 0.5 ml/tube) and control. The results showed that the effective repellent concentration were 0,05 ml/tube and 0,1 ml/tube which repelled  H. antonii  0,03 % . The effecive lethal concentration was  0,05 ml/tube which killed  H. antonii  57,50 %. Concentration used was  highly  toxic which killed l H. Antonii within  six hours so of the repellent nature was notobserved.

scholarly journals The Investigation of Decontamination Effects of Ozone Gas on Microbial Load and Essential Oil of Several Medicinal Plants

2013 ◽  
Vol 5 (1) ◽  
pp. 34-38 ◽  
Author(s):  
Razieh VALI ASILL ◽  
Majid AZIZI ◽  
Maasome BAHREINI ◽  
Hossein AROUIEE
Keyword(s):  
Medicinal Plants ◽  
Essential Oil ◽  
Block Design ◽  
Microbial Load ◽  
Mentha Piperita ◽  
Gas Treatment ◽  
Zataria Multiflora ◽  
Gas Concentration ◽  
Mold And Yeast ◽  
And Control

Today, Ozone as a disinfectant method, without putting on the harmful effects on human and plant products, it is alternative common methods for disinfection of plant material. The research as a factorial experiment was conducted on the basis of randomized complete block design with three replications and the effects of Ozone gas on decreasing the microbial load of some important medicinal plants include: Peppermint (Mentha piperita), Summer savory (Satureja hortensis), Indian valerian(Valeriana wallichii), Meliss (Melissa officinalis) and Iranian thyme (Zataria multiflora) were investigated. Medicinal plants leaves were treated with Ozone gas concentration 0.3, 0.6 and 0.9 ml/L at times of 10 and 30 then total count, coliform and mold and yeast of the samples were studied. The result showed that Ozone gas decreases microbial load of medicinal plants samples. But Ozone gas and Ozone gas in medicinal plants interaction effect had no effect on essential oil content. The lowest and the highest of microbial load were detected in samples treated with concentration of 0.9 ml/L of Ozone gas and control respectively. The highest and the lowest of microbial load were observed in Iranian thyme and Indian valerian respectively. Also result showed that Ozone gas treatment for 30 min had the greatest of effect in reducing the microbial load and 0.9 ml/L Ozone gas concentration had the lowest of microbial load. Results of this survey reflect that the use of Ozone as a method of disinfection for medicinal plants is a decontamination.

PSXIV-14 Supplementation of high-grain diet with red osier dogwood affects in vitro digestibility and fermentation characteristics

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 477-477
Author(s):  
Wenzhu Yang
Keyword(s):  
Control Diet ◽  
Antimicrobial Properties ◽  
Positive Control ◽  
Gas Production ◽  
In Vitro Digestibility ◽  
Randomized Design ◽  
Rumen Acidosis ◽  
Barley Silage ◽  
And Control

Abstract Red osier dogwood (ROD) is a native shrub plant rich in phenolic compounds with antimicrobial properties. The objective of this study was to evaluate the effects of substituting barley silage with either raw ROD or ROD extract (RODE) in high-grain (HG) diet under a low media pH (5.8) on gas production (GP), dry matter (DM) disappearance (DMD) and fermentation characteristics in batch cultures. The study was a completely randomized design with 4 treatments: 1) control diet (10% barley silage and 90% barley concentrate, DM basis), control diet supplemented with 2) monensin (30 mg/kg diet DM; positive control), 3) substitution of 3% ROD or 4) 3% RODE for an equal portion of silage. Inoculum was obtained from 2 ruminally fistulated beef heifers offered the HG diet. Substrate ground (1 mm) was incubated for 24 h and the experiment repeated twice. The GP did not differ among treatments (147 ml/g DM), but the DMD differed (P < 0.02) at highest for control (69.4%), lowest for ROD (58.4%) and intermediate for other treatments (64.1%). Total volatile fatty acid (VFA) concentration (mM) tended (P< 0.08) to be lower with ROD (80.5) and monensin (80.1) than control (83.9). Acetate proportion was greater (P = 0.02) with ROD (46.2%) and RODE (46.9%) than control (42.4%) and monensin (42.3%). However, the propionate proportion was greater (P = 0.05) with monensin (32.1%) than other treatments (averaged 30.1%). Consequently, acetate to propionate ratio (A:P) of ROD (1.52) and RODE (1.56) was higher than monensin (1.32; P < 0.01) and control (1.44; P < 0.08). Differences in variables measured between ROD and RODE were minimal. These results indicated that the decreased DMD along with increased A:P with addition of ROD or RODE suggests that both ROD and RODE may be beneficial to HG fed cattle for reducing risk of rumen acidosis without negatively impacting fibre digestion.

scholarly journals In-vitro anti-cholinesterase activity of essential oil from four tropical medicinal plants

2015 ◽  
Vol 2 ◽  
pp. 850-857 ◽  
Author(s):  
I.A. Owokotomo ◽  
O. Ekundayo ◽  
T.G. Abayomi ◽  
A.V. Chukwuka
Keyword(s):  
Medicinal Plants ◽  
Essential Oil ◽  
Cholinesterase Activity

scholarly journals In vitro antimicrobial activity and alternative control of anthracnose in papaya

2018 ◽  
Vol 85 (0) ◽  
Author(s):  
Héber Ferreira dos Reis ◽  
Lilian Maria Arruda Bacchi ◽  
Silvana de Paula Quintão Scalon ◽  
Jasna Karoliny Pereira Flores
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Syzygium Aromaticum ◽  
Alternative Control ◽  
Ginger Extract ◽  
Cinnamomum Zeylanicum ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
And Control

ABSTRACT: We evaluated the efficacy of natural products in the control of papaya anthracnose, in vitro and in vivo. The in vitro experiments for evaluation of mycelial growth used a completely randomized 10 × 4 factorial design (treatments × evaluation periods) with eight replicates, with sporulation evaluated at the end of the experiment. The treatments involved the use of aqueous extract at concentrations of 5 and 15% for Syzygium aromaticum (L.) Merr. & Perry (clove), Cinnamomum zeylanicum Breym (cinnamon), and Zingiber officinalis Rox. (ginger); 1 and 3% chitosan; the fungicide Prochloraz at 100 µg.mL-1; and a control (no treatment). For evaluating conidia germination, we used six treatments with five replicates. The treatments included 7.5% of each extract (clove, cinnamon, and ginger), 1.5% chitosan, and 50 µg.mL-1 of Prochloraz. For the in vivo experiment, “Formosa” papaya “Tainung 1” was used in a completely randomized design, with six treatments and four replicates to evaluate the severity of anthracnose caused by Colletotrichum gloeosporioides. The fruits were treated by immersion for 5 min with 15% clove, cinnamon, and ginger extracts, 8% chitosan, and control with distilled water, and immersion for 2 min in Prochloraz (33.75 g a.i./100 L). The treatments with 15% clove extract and 8% chitosan were effective in all evaluations, resulting in a viable alternative to the fungicide Prochloraz. The treatments with ginger extract were less effective and those with cinnamon offered intermediate control.

scholarly journals In vitro fungitoxic potential of Lippia gracilis (Schauer) essential oil against phytopathogens

2020 ◽  
pp. 667-674
Author(s):  
Kevison Romulo da Silva França ◽  
Flavia Mota de Figueredo Alves ◽  
Tiago Silva Lima ◽  
Alda Leaby dos Santos Xavier ◽  
Plínio Tércio Medeiros de Azevedo ◽  
...  
Keyword(s):  
Essential Oil ◽  
Mycelial Growth ◽  
Fungal Growth ◽  
Fungal Species ◽  
Phytopathogenic Fungi ◽  
Negative Control ◽  
Growth Speed ◽  
Randomized Design

This study evaluates the in vitro effects of Lippia gracilis essential oil on the mycelial growth of phytopathogenic fungi. Experiments were carried out using a completely randomized design to assess the effects of eight treatments. Five replicates were evaluated for each experimental group. The essential oil was incorporated into the potato dextrose culture medium and poured into Petri dishes. Treatments were comprised of different concentrations of the oil (0.0125, 0.025, 0.05, 0.1, and 0.2%), a negative control (0.0%), and two positive controls (commercial fungicides). The plates were inoculated with fungi including Colletotrichum gloeosporioides, C. musae, C. fructicola, C. asianum, Alternaria alternata, A. brassicicola, Fusarium solani, F. oxysporum f. sp. cubense, and Lasiodiplodia theobromae and were incubated for seven days at 27 ± 2°C. The following variables were measured to verify the differences observed among treatments: percentage of mycelial growth inhibition and index of mycelial growth speed. All concentrations of L. gracilis oil inhibited the mycelial growth of the fungal species evaluated. The complete inhibition was observed between concentrations of 0.0125 and 0.1%. Treatment with oil inhibited fungal growth with similar, or even greater, efficiency than commercial fungicides.. We recommend the development of in vivo tests to verify whether L. gracilis essential oil can protect against fungal disease in live plants.

scholarly journals Compatibility Between Beauveria bassiana (Bals.) and Neem Extract against Brown Plant-Hopper (Nilaparvata lugens Stal.)

2019 ◽  
Vol 4 (1) ◽  
pp. 9
Author(s):  
Nova Laili Wisuda ◽  
Tri Harjaka ◽  
Indah Dwi Prasetyaningrum
Keyword(s):  
Beauveria Bassiana ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Spore Density ◽  
Neem Extract ◽  
Randomized Design ◽  
Speed Up ◽  
Nilaparvata Lugens Stål ◽  
And Control

Beauveria bassiana fungi and neem extract as botanical insecticide can be combined to get the synergizing effect to control brown planthopper (BPH). This study was conducted to determine the best combination between the spore density of B. bassiana and concentration of neem extract to control BPH effectively. The two factorial treatment consisted concentration of B. bassiana (106  Colony Forming Unit (CFU) and 107 CFU) and concentration of neem extract (5%, 10%, 15%), and control.  The treatments were assigned in Completely Randomized Design with three replications. The data were analyzed using ANOVA and  LSD at 5%. The test was conducted in vitro and bio assay of testing insects, BPH, was conducted using dipping method.  B. bassiana was suitable with neem extract with T value ranged from 78.58- 90.38. The mortality of BPH occurred on day 5 after application. The highest mortality (91.67%) occurred on the treatment with B. bassiana 107 CFU + neem extract 10%. The shortest LT50 was found at the treatment of B. bassiana 106 CFU+  neem extract 15%  (2.74 days) but not significantly different from B. bassiana 107 CFU + neem extract 15% (2.76 days). B. bassiana spores combined with neem extract are assumed to speed up the mortality of BPH by increasing the concentration.

scholarly journals In-Vitro Effectiveness of Ethanol Extract of Permot Leaf (Passiflora foetida Linn.) Towards Mortality of Rhipicephalus sanguineus Larvae

2020 ◽  
Vol 4 (2) ◽  
pp. 61
Author(s):  
Intan Nurcahya ◽  
Ratna Damayanti ◽  
Iwan Sahrial Hamid ◽  
Agus Wijaya
Keyword(s):  
Ethanol Extract ◽  
Rhipicephalus Sanguineus ◽  
Probit Analysis ◽  
Lethal Concentration ◽  
Extract Concentration ◽  
Randomized Design ◽  
Lethal Time ◽  
Completely Randomized Design ◽  
Passiflora Foetida

This study had three fold objective which were finding the effectiveness of ethanol extract of permot leaf (Passiflora foetida Linn.) towards mortality of Rhpicephalus sanguineus (R. sanguineus) larvae in vitro and also identifying lethal concentration and lethal time. Completely randomized design was used with six treatments and four replications. Five R. sanguineus were used in the treatment for all replication. The observation of mortality was done every ten minutes in five hours. Rhpicephalus sanguineus larvae were dead if there was no movement. The collected data was analyzed using ANOVA factorial and followed by Duncan’s test. The obtained data showed that ethanol extract of permot’s leaf ethanol extract was effective toward mortality of R. sanguineus larvae in vitro. The suspension of 10% extract concentration of permot leaf ethanol extract has a similar effectiveness as Noticks®. Probit analysis stated that LC50 can be reached by using the 4% concentration in two hours and 2.4% concentration in five hours. LC95 can be reached by using the 6.5% concentration in two hours and 3.93% concentration in five hours. The quickest LT50 can be reached in 0.91 hours and LT95 ­2.37 hours after treatment with 10% concentration.

scholarly journals Penambahan Glutathione pada Medium Fertilisasi Efektif Mendukung Pembentukan Pronukleus dan Perkembangan Awal Embrio Sapi (SUPPLEMENTATION OF GLUTATHIONE IN FERTILIZATION MEDIUM EFFECTIVELY SUPPORT NORMAL PRONUCLEUS FORMATION AND EARLY BOVINE EMBRYONIC DE

2017 ◽  
Vol 18 (3) ◽  
pp. 327
Author(s):  
Aras Prasetiyo Nugroho ◽  
Iman Supriatna ◽  
Mohamad Agus Setiadi
Keyword(s):  
Tissue Culture ◽  
Embryonic Development ◽  
Culture Medium ◽  
Fertilization Rate ◽  
Tissue Culture Medium ◽  
Randomized Design ◽  
Oviduct Fluid ◽  
And Control ◽  
Bovine Oocytes

The objective of this study was to determine fertilization rate effectiveness and early embryonic development competency with glutathione (GSH) supplementation in fertilization medium and culture This study consisted of two experiments comprising each of the four treatment and six repetitions with completely randomized design (CRD) using 651 oocytes. In the first experiment, a total of 317 bovine oocytes were matured in tissue culture medium (TCM) 199 at incubator 5% CO2 with temperature 39 ºC for24 h, then fertilized with sperm separated by swim up technique. Oocyte and sperms were incubated in fertilization medium supplemented with 0.25 mM, 0.50 mM, 1.00 mM GSH. In the second experiment, bovine oocytes were matured in maturation medium and fertilized with same procedure as mentioned before, then cultured in modified synthetic oviduct fluid (mSOF) with the following treatment: supplementation GSH only in fertilization medium (T1), supplementation GSH only in culture medium (T2), and supplementation GSH in both fertilization and culture medium (T3), while control not supplementation GSH (T0). Result of the first experiment showed that supplementation 1.00 mM GSH in fertilization medium can increase higher normal pronucleus (2PN) formation (86,9%) compared to other treatments, 0.50 mM (80.3%), 0.25 mM (73.8%), and control (58.9%) (P<0.05). In the second experiment showed that early bovine embryonic development on 2nd day cultured which reached 5-8 cell on treatment T1 (56.0%) and T3 (53.6%) were higher (P<0.05) compared to treatment T2 (26.2%) and T0 (control) (31.3%). Result of the other were showed that early bovine embryonic development on 4th day cultured which reached 9-16 cell on treatment T1 (26.2%) and T3 (27.4%) were higher (P<0.05) compared to that T2 (11.9%) and T0 (control) (10.8%). In conclusion, 1.00 mM GSH supplementation in medium was more effective in supporting normal pronucleus formation and early fertilization bovine embryonic development compared to in culture medium. ABSTRAK Penelitian ini bertujuan untuk mengetahui tingkat fertilisasi dan kompetensi perkembangan awal embrio sapi dengan penambahan glutathione (GSH) pada medium fertilisasi in vitro (IVF) dan kultur in vitro (IVC). Penelitian ini terdiri atas dua penelitian yang terdiri dari masing-masing empat perlakukan dan enam kali ulangan dengan rancangan acak lengkap (RAL) menggunakan 651 oosit. Penelitian I, sebanyak 317 oosit sapi dalam tissue culture medium (TCM) 199 dimatangkan pada inkubator 5% CO2 dan suhu 39°C selama 24 jam, kemudian difertilisasi dengan spermatozoa yang telah diseleksi menggunakan teknik swim up. Oosit dan spermatozoa diinkubasi pada medium fertilisasi dengan penambahan 0,25 mM, 0,50 mM, dan 1,00 mM GSH. Penelitian II, sebanyak 334 oosit sapi dimatangkan pada medium pematangan dan difertilisasi, kemudian dikultur pada medium modified synthetic oviduct fluid (mSOF), dengan perlakuan: penambahan GSH hanya pada medium fertilisasi (T1), penambahan GSH hanya pada medium kultur (T2), dan kombinasi penambahan GSH pada medium fertilisasi dan kultur (T3). Hasil penelitian I, menunjukkan bahwa penambahan 1,00 mM GSH pada medium fertilisasi dapat meningkatkan pembentukan pronukleus normal (2PN) yang lebih tinggi (86,9%) dibandingkan dengan perlakuan yang lain yaitu 0,50 mM (80,3%), 0,25 mM (73,8%), dan kontrol (58,9%) (P<0,05). Penelitian II menujukkan bahwa perkembangan awal embrio sapi pada hari ke-2 kultur yang mencapai pembelahan 5-8 sel pada perlakukan T1 (56,0%) dan T3 (53,6%) lebih tinggi (P<0,05) dibandingkan dengan perlakuan T2 (26,2%) dan T0 (kontrol) (31,3%). Hasil penelitian lain menunjukkan bahwa perkembangan awal embrio sapi pada hari ke-4 kultur yang mencapai pembelahan 9-16 sel pada perlakuan T1 (26,2%) dan T3 (27,4%) lebih tinggi dibandingkan dengan perlakukan T2 (11,9%) dan T0 (kontrol) (10,8%) (P<0,05). Dapat disimpulkan bahwa penambahan 1,00 mM GSH pada medium fertilisasi lebih efektif dalam mendukung pembentukan pronukleus normal dan perkembangan awal embrio sapi dibandingkan pada medium kultur.

scholarly journals Potential effects of essential oil from Zanthoxylum limonella seeds against Tribolium castaneum (Coleoptera: Tenebrionidae)

2020 ◽  
pp. 1920-1925
Author(s):  
Ruchuon Wanna ◽  
Bunyaporn Satongrod
Keyword(s):  
Essential Oil ◽  
Tribolium Castaneum ◽  
Insecticidal Activity ◽  
Insect Pests ◽  
Randomized Design ◽  
Mass Spectrometry Technique ◽  
Spectrometry Technique ◽  
Gas Chromatograph Mass Spectrometry ◽  
And Control ◽  
Chemical Pesticides

Essential oils of plants with insecticidal properties have been considered as replacements for synthetic chemical pesticides to combat and control insect pests. The chemical composition of the essential oil from dried seeds of Zanthoxylum limonella Alston was determined by the Gas Chromatograph-Mass Spectrometry technique. Insecticidal activity was investigated on Tribolium castaneum Herbst using impregnated filter paper with six concentrations of essential oil at 0, 1, 2, 3, 4, and 5% for adults and larvae mortality tests, and 0, 2, 4, 6, 8 and 10% for egg mortality. All experiments were performed under completely randomized design (CRD) with four replications at 30±5°C and 70±5% relative humidity in 16:8 hours light/dark cycle. A total of 83 components were identified. The principal compounds in the essential oil of Z. limonella were beta-pinene (19.65%), 9-octadecanone (18.80%), D-limonene (9.76%), alpha-fenchene (8.48%), p-mentha-1,5,8-triene (7.16%), 1,8-cineole (6.88%), gamma-terpinene (5.46%), terpinen-4-ol (3.81%), linalool (2.73%), alpha-thujene (1.34%), decanal (1.32%), alpha-phellandrene (1.20%) and linalyl propionate (1.13%). Insecticidal activity presented that 5% of essential oil had the highest effect against T. castaneum at 120 h for adults and 48 h for larvae, while 10% of essential oil at 14 days obtained 100% mortality against eggs of T. castaneum. Results indicated that the essential oil of Z. limonella from dried seeds showed potential for use in the control of T. castaneum

scholarly journals Perbaikan Pembungaan Pamelo melalui Aplikasi Strangulasi dan Zat Pemecah Dormansi

2017 ◽  
Vol 7 (3) ◽  
pp. 139
Author(s):  
Slamet Susanto ◽  
Maya Melati ◽  
Herik Sugeru
Keyword(s):  
Carbohydrate Content ◽  
Research Station ◽  
Dormancy Breaking ◽  
Randomized Design ◽  
Breaking Dormancy ◽  
Two Factors ◽  
The Wire ◽  
Research Experiment ◽  
Agriculture Research Institute ◽  
And Control

<p>ABSTRACT<br />Pummelo productivity is still low, therefore efforts should be made to increase its flowering and production. The objective of study was to determine the effectiveness of strangulation combined with the application of breaking dormancy substances to improve flowering of pummelo. The experiment was conducted in January to November 2015, at the Cikabayan Experimental Research Station, IPB. The carbohydrate and nitrogen analysis was done in laboratory of Postharvest Agriculture Research Institute, Bogor. Three-year-old pummelo grown in field was subjected for this research. Experiment used a completely randomized design (CRD) with two factors. The first factor was treatment to stimulate flowering, consisted of 3 levels i.e. single strangulation, double strangulation and control, and the second factor was the use of dormancy breaking substances, consisted of 3 types i.e. KNO3, Ethepon and BAP. Strangulation was performed by pressing the wire with a diameter of 2.0 mm into stem as deep as the diameter of the wire. Strangulation was done simultaneously and then released after 3 months. Dormancy breaking substance was applied immediately after releasing the wire for strangulation with the concentration of 200 ppm KNO3, 100 ppm Ethepon or 100 ppm BA. Treatments were replicated 4 times. The results showed that single and double strangulation treatments for 3 months were an effective way to induce flowering of young pummelo trees. Double strangulation produced more flowers as compared to single strangulation.<br />Increased carbohydrate content and C/N ratio in leaves were observed on flower induced trees. Strangulation treatment for 3 months can increase flowering of young pummelo trees. Application of dormancy breaking substances did not have any effect on flowering induction in pummelo.</p><p>Keywords: carbohydrate content, dormancy breaking substance, flower induction, pummelo, strangulation</p><p>ABSTRAK<br />Produktivitas pamelo masih rendah sehingga perlu upaya peningkatan pembungaan dan produksinya. Penelitian ini bertujuan untuk mengetahui efektivitas strangulasi yang dikombinasikan dengan aplikasi zat pemecah dormansi dalam meningkatkan pembungaan jeruk pamelo. Penelitian dilaksanakan pada bulan Januari sampai Nopember 2015, bertempat di Kebun Percobaan IPB Cikabayan. Analisis karbohidrat dan nitrogen dilakukan di laboratorium BB Pasca Panen, Bogor. Percobaan menggunakan tanaman jeruk pamelo “Nambangan’ berumur 3 tahun dengan Rancangan Acak Lengkap (RAL) dua faktor. Sebagai faktor pertama yaitu perlakuan untuk menstimulasi pembungaan, terdiri atas 3 taraf perlakuan yaitu perlakuan strangulasi tunggal, strangulasi ganda dan kontrol, dan sebagai faktor kedua adalah penggunaan zat pemecah dormansi terdiri atas 3 jenis yaitu KNO3, Ethepon dan BAP. Masing-masing perlakuan diulang 4 kali sehingga terdapat 36 unit percobaan. Strangulasi dilaksanakan dengan melilitkan kawat dengan diameter 2.0 mm pada batang dengan menekan kawat ke batang sedalam diameter kawat tersebut. Strangulasi dilakukan serentak pada batang, strangulasi dilepas setelah 3 bulan kemudian. Zat pemecah dormansi diaplikasikan segera setelah pelepasan kawat strangulasi dengan konsentrasi masing-masing 200 ppm untuk KNO3, 100 ppm untuk Ethepon atau 100 ppm untuk BAP. Hasil penelitian menunjukkan perlakuan strangulasi selama 3 bulan merupakan cara efektif menginduksi pembungaan tanaman jeruk pamelo. Perlakuan strangulasi ganda menghasilkan bunga lebih banyak dibandingkan dengan strangulasi tunggal. Tanaman yang telah terinduksi menunjukkan tingginya kandungan karbohidrat pada tajuk tanaman sehingga meningkatkan rasio C/N. Perlakuan zat pemecah dormansi tidak memberikan pengaruh yang nyata terhadap pembungaan jeruk pamelo.<br />Kata kunci: kandungan karbohidrat, pamelo, pembungaan, strangulasi, zat pemecah dormansi</p>

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