scholarly journals Effect of Annona muricata L. Leaf Ethanol Extract in CCl4 Hepatitis Rat Model

2021 ◽  
Vol 8 (2) ◽  
Author(s):  
Kuswinarti Kuswinarti ◽  
Ilham Asrori Azka ◽  
Dwi Prasetyo
Keyword(s):  
Cell Membrane ◽  
Intact Cell ◽  
Antioxidant Properties ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Negative Control ◽  
Significant Inhibition ◽  
Control Group ◽  
Annona Muricata ◽  
Anti Oxidant

Background: Liver can be damaged by various factors, including the oxidative stress mechanism. This damage can be inhibited by the application of anti-oxidant. The Annona muricata L. leaf is known to have antioxidant properties. This study aimed to explore the effect of Annona muricata L. leaf extract against hepatitis in rats.Methods: An experimental study was performed on 25 male Wistar rats that were given Annona muricata L. leaf ethanol extract at 200 mg/kgBW, 400 mg/kgBW, and 600 mg/kgBW for eight days. The CCl4 was administrated on the eighth day and the rats were laparotomized on the next day. The average number of normal hepatocytes was counted in one field of view. This study was conducted at the laboratory of Pharmacology and Therapy of the Faculty of Medicine, Universitas Padjadjaran in September–October 2012.Results: The negative control group showed an intact cell membrane, homogenous cytoplasm, and round nucleus, whereas the positive control group showed necrosis marked by a ruptured cell membrane, vacuolated cytoplasm, and degenerated nucleus. The average number of normal hepatocytes counted in the negative control and the positive group was 404.91±34.09 cells and 23.16±7.84 cells, respectively. In the treatment group, the normal hepatocytes were 95.91±20.96 cells (200 mg/kgBW), 74.53±13.59 cells (400 mg/kgBW) and 46.84±8.46 cells (600 mg/kgBW). Statistically significant inhibition was observed (p<0.05).Conclusions: The Annona muricata L. leaf ethanol extract can inhibit liver tissue damages in hepatitis model rats, possibly due to the presence of acetogenins as an anti-oxidant agent.

scholarly journals THE ANTI-OXIDANT ACTIVITIES OF ETHANOL EXTRACT OF MAHOGANY (Sweitenia mahagoni Jacq.) SEEDS IN THE LIVER TISSUES OF DIABETIC EXPERIMENTAL RATS

2017 ◽  
Vol 11 (2) ◽  
Author(s):  
Eka Prasetiawan ◽  
I Ketut Mudite Adnyane ◽  
Tutik Wresdiyati
Keyword(s):  
Ethanol Extract ◽  
Thiobarbituric Acid ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substance ◽  
Sprague Dawley ◽  
Anti Oxidant ◽  
Oxidant Content ◽  
Liver Tissues

The aim of this research was to analyze the anti-oxidant activities of ethanol extract of mahogany seeds on the liver tissues of diabetic experimental rats to overcome oxidative stress condition. This study used male Rattus norvegicus strain Sprague Dawley which were divided into 5 groups: (i) negative control group (K-), (ii) positive control group/diabetes mellitus (DM) (K+), (iii) DM group treated with 500 mg/kg BW ethanol extract of mahogany seeds (EM), (iv) DM group treated with acarbose (KO), and (v) non-DM group treated with 500 mg/kg BW ethanol extract of mahogany seed (KE). Alloxan with dose of 110 mg/kg BW was used to induce diabetes in rats. The treatments were conducted for 28 days. At the end of treatment, the liver tissues were collected and analyzed for malondialdehyde (MDA) content using thiobarbituric acid reactive substance (TBARS) method and anti-oxidant cooper zinc superoxide dismutase (Cu,Zn-SOD) content using immunohistochemical technique. The result showed that level of MDA were significantly different (P<0.05) in K-; K+; EM; KO; and KE with values were 0.81±0.17; 1.19±0.26; 1.10±0.04; 0.95±0.13; and 0.92±0.0 µmol/gram, respectively. The anti-oxidant content (Cu, Zn-SOD) of rat liver tissue in K+ lower than in K-, while anti-oxidant content in EM and KE were higher compared to K+. The study concluded that ethanol mahogany seeds extract decreased blood glucose levels and increased the anti-oxidant status in the liver tissues of diabetic rats.

Efek Ekstrak Etanol Kulit Petai (Parkia speciosa Hassk) Terhadap Penurunan Kadar Glukosa Darah Mencit Jantan

2018 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Verawaty Verawaty ◽  
Dhea Claudia Novel
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Male Mice ◽  
Glucose Levels ◽  
The Third ◽  
Blood Glucose Levels

<p>Penelitian ini bertujuan untuk melihat pengaruh pemberian ekstrak etanol kulit petai (Parkia speciosa Hassk) terhadap penurunan kadar glukosa darah mencit jantan yang diinduksi aloksan. Hewan percobaan dibagi atas 5 kelompok diantaranya kelompok kontrol negatif, kelompok kontrol positif,dosis I (280 mg/kgBB mencit), dosis II (560 mg/kg BB mencit), dosis III (840 mg/kg BB mencit). Penelitian dilakukan selama 21 hari. Persentase penurunan kadar glukosa darah mencit jantan setelah diberikan ekstrak etanol kulit petai pada hari ke-21 adalah dosis I (77,52 %) lebih besar dibandingkan dengan dosis II (69,5 %) dan dosis III (73,37 %). Data yang diperoleh dianalisis dengan uji Two Way Anova dengan program SPSS 17. Hasil penelitian ini menunjukkan bahwa pemberian ekstrak etanol kulit petai untuk tiga variasi dosis menyatakan perbedaan yang bermakna secara statistik terhadap penurunan kadar glukosa darah mencit jantan.</p><p><em>Petai (Parkia speciosa Hassk) has a compound β-sitosterol and stigmasterol that have efficacy to decreased blood glucose levels. This study aimed to determine the effect of ethanol extract of petai peel for decrease blood glucose levels of male mice induced by alloxan. Experimental animals were divided into 5 groups including negative control group, positive control group, the first dose (280 mg/kg in mice), the second dose (560 mg/kg in mice), the third dose (840 mg/kg in mice). The study was conducted for 21 days. After 21 days, the result found that the percentage of blood glucose levels after the male mice given the ethanol extract of petai peel was, the first dose (77.52%) biger than the second dose (69.5%) and the third dose (73.37%). The data obtained were analyzed by Two Way ANOVA using SPSS 17. The results showed that have signicantly difference between three dose variation of ethanol extract of petai peel in blood glucose levels.</em></p>

Effect of Chayote (Sechium Edule Jacq. Swartz) Extract on Level of Interleukin-8 in Wistar Rats with Aspirin-Induced Gastritis

2020 ◽  
Author(s):  
Hendrika Andriana Silitonga ◽  
◽  
Gontar Alamsyah Siregar ◽  
Rosita Juwita Sembiring ◽  
Marline Nainggolan ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Wistar Rats ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Interleukin 8 ◽  
Control Group ◽  
Sechium Edule

ABSTRACT Background: Recent studies showed that Interleukin-8 (IL-8), activated cytokine immune response which plays an important role in the development of acute and chronic gastritis. Harmless anti-inflammatory therapeutic alternatives have been proposed, for example, the consumption of Sechium Edule Jacq. Swartz (chayote). Antioxidant (flavonoid) and cell regeneration (alkaloid) agents were found in chayote. This study aimed to determine the effect of chayote Sechium Edule Jacq. Swartz extracts on the level of IL-8 in Wistar rats with aspirin- induced gastritis. Subjects and Method: This was a randomized controlled trial (RCT) conducted at the laboratory of Mathematics and Natural Science, Universitas Sumatra Utara from January to February 2020. A total of 35 male Wistar rats was selected for this study and randomly allocated into 7 groups: (1) Negative control; (2) Positive control; (3) 100 mg/ kg BW chayote ethanol extract ; (4) 200 mg/kg BW chayote ethanol extract; (5) 100 mg/ kg BW chayote ethyl acetate fraction; (6) 200 mg/kg BW chayote ethyl acetate fraction; and (7) 20 mg omeprazole. The rats in positive control and treatment groups were induced with aspirin (200mg/ kg BW). The negative control group received no intervention. The dependent variable was level of IL-8 measured by ELISA. The independent variables were treatment status. The data were analyzed by One Way Anova and post hoc test. Results: The mean differences of IL-8 level were not statistically significant between study groups (p= 0.327). Mean of IL-8 level was higher in positive control group (Mean= 160.80; SD= 6.90) than in negative control group (Mean= 141.20; SD= 10.98). The lowest IL-8 level was in 100mg/ kg BW chayote ethanol extract group (Mean= 149.94; SD= 40.4), followed by 200mg/ kg BW (Mean= 152.4; SD= 30.73) and 100mg/ kg BW (Mean= 164.60; SD= 25.04) chayote ethyl acetate fraction groups, 20 mg omeprazole group (Mean= 170.60; SD= 21.58), and 200 mg/ kg BW chayote ethanol extract group (Mean= 176.80; SD= 10.98). Conclusion: The low dose (100mg/ kg BW) chayote ethanol extract has the most potential antiinflammation effect on in vitro gastritis with the lowest IL-8 level of all doses of chayote ethanol extract, chayote ethyl acetate fraction, and omeprazole. Keywords: antiinflammation, IL-8, chayote ethanol extract, ethyl acetate fraction, omeprazole, aspirin induced gastritis Correspondence: Hendrika Andriana Silitonga. Department of Histology, Faculty of Medicine, Universitas Methodist Indonesia. Email: [email protected]. Mobile: +6281361430688. DOI: https://doi.org/10.26911/the7thicph.05.35

scholarly journals Efek Imunomodulator Ekstrak Etanol Spons Melophlus sarasinorum Terhadap Aktivitas Fagositosis Sel Makrofag Pada Mencit Jantan Balb/C

2019 ◽  
Vol 5 (2) ◽  
pp. 147-157
Author(s):  
Wahyuni Wahyuni ◽  
Muhammad Ilyas Yusuf ◽  
Fadhliyah Malik ◽  
Adryan Fristiohady Lubis ◽  
Astrid Indalifiany ◽  
...  
Keyword(s):  
Cell Activity ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Macrophage Cell ◽  
Macrophage Phagocytosis ◽  
Post Hoc ◽  
Phagocytosis Activity

Immunomodulator is an ingredient or drug that can modulate immune system functions and activities. This study was conducted to determine the effect of ethanol extract of Melophlus sarasinorum sponge on macrophage phagocytosis activity. Twenty four male mice balb/c were divided into six groups. The first group received 100 mg/kg of ethanol extract of Melophlus Sarasinorum sponge, the second group received 200 mg/kg of ethanol extract of Melophlus sarasinorum sponge, the third group received 300 mg/kg of ethanol extract of Melophlus Sarasinorum sponge and the fourth group received 400 mg/kg of ethanol extract of Melophlus Sarasinorum sponge. The positive control group received Phyllanthus niruri linn extract (Stimuno®) 0,13 mg/g and the negative control group received NaCMC 0,5%. The extract was orally administered from first day to seventh day. On the eighth day, each of the mice was injected Staphylococcus aureus bacteria (SA) 0.5 mL intraperitoneally. Macrophage cell activity is calculated from smears of peritoneal fluid of mice. Increased doses of ethanol extract of Melophlus sarasinorum sponge increase the amount of macrophage phagocytosis activity that are 25,25% (negative control), 61,5% (positive control), 55,75% (100 mg/kg), 60,75% (200 mg/kg), 62,25% (300 mg/kg) dan 66,25% (400 mg/kg). The results showed that the ethanol extract of Melophlus sarasinorum sponge has the potential as immunomodulator at a doses of 300 mg/kgBB and 400 mg/kg with no significantly different effectiveness with positive control in increasing macrophage cell phagocytosis activity based on the result of post-hoc statistical test of Tukey (sig.> 0,05).  

scholarly journals Anti-nociceptive Potential of Ethanol Extract of Terminalia macroptera Guill&Perr (Combretaceae) Stem Bark in Mice

2021 ◽  
Vol 16 (2) ◽  
pp. 13-21
Author(s):  
S.A. Atunwa ◽  
M.O. Amali ◽  
S.O. Lawal ◽  
S.O. Usman ◽  
A.I. Olapade
Keyword(s):  
Acetic Acid ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Stem Bark ◽  
Negative Control ◽  
Flowering Plant ◽  
Control Group ◽  
Group I ◽  
Tail Immersion ◽  
Dose Dependent

Background: Terminalia macroptera Guill. &Perr. (Combretaceae) is a flowering plant with several ethno-medicinal claims. However, the dearth of information on its analgesic property has necessitated this study.Objectives: to evaluate the anti-nociceptive potential of ethanol extract of Terminalia macroptera stem bark (TMSB) in mice.Materials and Methods: Male and female mice of weight range 22 – 25g were randomly allotted into seven groups (n= 5) and treated as follows: Group I received 0.5 mL distilled water orally (negative control), Groups II-V were orally administered ethanol extract of T. macroptera stem bark (TMSB) at 50, 100, 200, and 400 mg/kg respectively while groups VI-VII received piroxicam 10 mg/kg and pentazocine 2 mg/kg intraperitoneally respectively as standards. The same treatment pattern was adopted for both pain models: tail immersion and acetic acid-induced writhing assays. Data were expressed as mean ± standard error of mean (SEM) using two-way analysis of variance (ANOVA) followed by Tukey’s and Bonferroni's multiple comparisons tests with p < 0.05 taken as significance.Results: The ethanolic extract of Terminalia macroptera stem bark showed significant dose-dependent anti-nociceptive activity at 100 and 400 mg/kg (2.95±0.41 and 2.9±0.31 respectively) 60 min post-treatment compared to the negative control group in the tail immersion test. Significant inhibition of nociception (0.20±0.20) was obtained at 400 mg/kg compared to the negative control group in the acetic acid-induced writhing test.Conclusions: The ethanol extract of Terminalia macroptera stem bark exhibited dose-dependent anti-nociceptive potential in both tail immersion and acetic acid-induced writhing assays in mice.

scholarly journals Analgesic Activity of Ethanol Extract of Temu Giring Rhizome (Curcuma heyneana Val & Zijp) in Mice

2018 ◽  
Vol 1 (2) ◽  
pp. 9-13
Author(s):  
Marianne ◽  
Khairunnisa ◽  
Wilda
Keyword(s):  
Analgesic Activity ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Significance Level ◽  
Treatment Groups ◽  
Infra Red ◽  
Thermal Induction

Temu giring (Curcuma heyneana Val & Zijp) is a traditional medicinal plant that is believed in community as an analgesic. The objective of this research was to determine the analgesic activity of the C. heyneana rhizome by using infra red (IR) thermal induction method in mice. Mice were divided into 7 groups. Group 1 served as negative control, group 2,3,4,5 served as treatment groups which is  given ethanolic extract of C. heyneana rhizome at  dose of 5, 25, 125, and 625 mg/kg respectively, group 6 and 7 served as  comparable groups, given antalgin 65 mg/kg and morphine sulphate 1.3 mg/kg respectively. The observation have been done, included to pain resistance of mice which exposed by infra red (IR) every 10 minutes for 80 minutes. The data were analyzed by ANOVA at the significance level of 95%. Ethanolic extract of C. heyneana at the doses of 25, 125, and 625 mg/kg had significant effect to reduce the pain compared to the negative control (p<0.05). Ethanolic extract of C. heyneana rhizome at dose of 125 mg/kg, had the same effect to antalgin 65 mg/kg  (p≥0.05), while the ethanolic extract of C. heyneana at the dose of 625 mg/kg had the same effect as morphine sulfate 1.3 mg/kg (p≥0.05). It can be concluded that ethanolic extract of C. heyneana rhizome has analgesic activity.   Keywords: temu giring, analgesic, Curcuma heyneana, rhizome

scholarly journals UJI EFEK ANALGESIK EKSTRAK ETANOL DAUN GEDI (Abelmoschus manihot (L.) Medik) PADA MENCIT (Mus musculus)

2013 ◽  
Vol 1 (1) ◽  
Author(s):  
Ristanti Pratiwi ◽  
Jimmy Posangi ◽  
Fatimawali .
Keyword(s):  
Analgesic Effect ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Maximum Effect ◽  
Control Group ◽  
Hot Plate ◽  
Thermal Pain ◽  
The Difference

Abstract: The objectives of the research were to find out the analgesic effect of giving Gedi (Abelmoschus manihot (L.) Medik) leaf ethanol extract orally on the number of writhing after thermal pain induction of mice. This research using 15 mice which is divided into 5 groups consisted of 1 negative control group given by the aquades, 1 positive control group given by the tramadol, and 3 experiment groups. Experiment group given by Gedi (Abelmoschus manihot (L.) Medik) leaf ethanol extract with the doses which different each other, that is 30 mg/30 g BW, 60 mg/30 g BW and 120 mg/30 g BW. Thermal pain induction was done by placing the mice on hot plate constant temperature of 550C. The mice gave respond in the way of lick its foot or even jumping. The data was collected using table, graphic and analyzed using one direction ANOVA model and it was continued with LSD test to find out the difference every treatment group. The result of analysis showed that gedi’s leaf ethanol extract have the analgesic effect and the maximum effect presented at gedi leaf ethanol extract dosage 60 mg/30 g BW. Keywords: Gedi’s leaf, analgesic effect    Abstrak: Tujuan penelitan ini yaitu menemukan efek analgesik dari pemberian ekstrak etanol daun gedi (Abelmoschus manihot (L.) Medik) peroral pada mencit yang kemudian diamati jumlah geliatnya setelah diinduksi panas. Penelitian ini menggunakan 15 ekor mencit yang dibagi 5 kelompok yang terdiri dari 1 kelompok kontrol negatif yang diberi aquades, 1 kelompok kontrol positif yang diberi tramadol, dan 3 kelompok eksperimen. Kelompok eksperimen diberi ekstrak etanol daun gedi dengan dosis yang berbeda-beda, yaitu 30 mg/30 g BB, 60 mg/30 g BB, dan 120 mg/30 g BB. Induksi nyeri berupa panas dilakukan dengan meletakkan mencit pada hot plate dengan suhu 550C . Mencit memberi respon berupa menjilat kaki dan atau melompat. Data disajikan berupa tabel, grafik dan menggunakan analisis statistik ANOVA yang dilanjutkan dengan LSD untuk menemukan perbedaan dari setiap kelompok. Hasil analisis menunjukkan bahwa ekstrak etanol daun gedi memiliki efek analgesik dan efek maksimumnya didapatkan pada dosis 60 mg/30 g BB. Kata kunci: Daun gedi, efek analgesik

scholarly journals Efek Antidiare Ekstrak Etanol Daun Kelor (Moringa oleifera L.) Pada Mencit Putih Jantan

2020 ◽  
Vol 6 (1) ◽  
pp. 35-39
Author(s):  
Rizal Fauzi ◽  
◽  
Annisa Fatmawati ◽  
Emelda Emelda ◽  
◽  
...  
Keyword(s):  
Body Weight ◽  
Intestinal Motility ◽  
Moringa Oleifera ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Extraction Process ◽  
Negative Control ◽  
Control Group ◽  
Average Value ◽  
Normal Individuals

Diarrhea is an increase frequency and decrease consistency of fecal when compared with the bowel condition of normal individuals. The high incidence of acute and chronic diarrhea encourages researchers to keep trying to find new antidiarrheal drugs, especially from plants. The leaves of Moringa (Moringa oleifera Lam) contain tannins which are polyphenolic compounds and has benefits as anantidiarrheal.This study aims to determine the activity of the ethanol extract of moringa leaves as an antidiarrheal by looking at intestinal motility. The extraction process was carried out by maceration method using ethanol 70%..The result research is the average value of the treatment ratio is smaller than the negative control group. The average value of the ratio of the group was given by ethanol extract of moringa leaves at a dose of 9.1 mg / 20 g body weight was 0.664; ethanol extract of moringa leaves at dose of 18.2 mg / 20 g body weight was 0.434 and ethanol extract of moringa leaves at dose of 36.4 mg / 20 g body weight was 0.389. From this value, it is known that the ethanol extract of Moringa leaves has an antidiarrheal effect by inhibiting intestinal motility. The effect of the ethanol extract of Moringa leaves in inhibiting intestinal motility is best at a dose of 36.4 mg / 20 g body weight with an average value ratio of 0.389.

scholarly journals Potential of Cocoa Extract (Theobroma cacao) in Inhibiting Erythrocyte Damage Induced by Physalia utriculus Venom

2020 ◽  
Vol 6 (3) ◽  
pp. 157
Author(s):  
Adinningtyas Intansari ◽  
Al Munawir ◽  
Laksmi Indreswari
Keyword(s):  
Theobroma Cacao ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Marine Biota ◽  
Erythrocyte Lysis ◽  
Theobroma Cacao L

Physalia utriculus is one of the invertebrate marine biota that is often found in Indonesia. Some symptoms of venoming due to jellyfish stings cause pain, itching, and hemolysis. In Indonesia, 13 cases of jellyfish stings were reported in 2005-2009 with three people dying in Java, Bali, and Bangka. Cocoa beans (Theobroma cacao L.) contain fat, carbohydrates, proteins, and polyphenol compounds that are useful as antioxidants. Polyphenols in the form of epicathechins, catechins, and procyanidins serve to provide protection to hemolysis. The purpose of this study was to determine the potential of ethanol extract of cacao (Theobroma cacao L.) in inhibiting the damage of erythrocyte induced by Physalia utriculus in vitro. This study used 28 samples of erythrocytes divided into seven groups, namely the normal control group, negative controls, and treatment with cocoa ethanol extract 0.2%, 0.1%, 0.04%, and 0.02%. Each subsequent group induced venom Physalia utriculus. The results showed that the average speed of erythrocyte lysis in the treatment group by giving cocoa ethanol extract 0.2%, 0.1%, 0.04%, and 0.02% respectively (seconds ± standard deviation) was 858,25 ± 94,44; 1.000,5 ± 159,93; 678,5 ± 19,71; and 1.006 ± 159,50. The mean speed of erythrocyte lysis in the negative control group was 1,025 ± 164.63 and the positive control group with the administration of N-Acetylcystein can last up to one hour after administration of venoms. Test for normality and homogeneity shows that data is normally distributed and homogeneous. One Way Annova analysis shows the significance value of p <0.05, then a post hoc analysis test was performed with the Bonferoni method to find out the differences in significance in each group. In this study it can be concluded that the administration of cocoa ethanol extract has no potential to inhibit erythrocyte damage that has been venomed by Physalia utriculus in vitro. Keywords: Physalia utriculus, cacao, erythrocyte damage

scholarly journals Potensi Minyak Sawit Merah Dan Karotenoid Sebagai Suplemen Antioksidan Dalam Pengujian Toleransi Glukosa Pada Tikus Putih (Preliminary Study)

2018 ◽  
Vol 1 (1) ◽  
pp. 251-256 ◽  
Author(s):  
Ahmad Gazali Sofwan Sinaga ◽  
Donald Siahaan ◽  
Kasmirul Ramlan Sinaga
Keyword(s):  
Blood Glucose ◽  
Palm Oil ◽  
Preliminary Test ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Distilled Water ◽  
Red Palm Oil ◽  
Dpph Method ◽  
Anti Oxidant

Minyak sawit merah (MSM) adalah salah satu produk olahan dari minyak kelapa sawit, dan merupakan sumber makanan yang kaya karotenoid sekitar 500 - 800 ppm. Konsentrat karotenoid (KK) juga merupakan produk yang diperoleh dari proses penghilangan minyak sehingga kadar karotenoid meningkat hingga 6000 - 7000 ppm. Penelitian ini bertujuan untuk mengetahui potensi penggunaan minyak sawit merah dan konsentrat karotenoid sebagai uji pendahuluan suplemen antioksidan dalam pengujian toleransi glukosa pada tikus putih. Pengujian aktivitas antioksidan dari minyak sawit merah dan konsentrat karotenoid diuji dengan metode DPPH dengan masa inkubasi selama 60 menit. Pengujian efektivitas antihiperglikemia dilakukan menggunakan hewan percobaan (n=40) yang dibagi menjadi 8 grup: grup kontrol negatif (akuades), kontrol positif (glibenklamid 10 mg), tiga grup diberi minyak sawit merah dengan dosis 1, 2, dan 3 ml, dan tiga grup diberi konsentrat karotenoid dengan dosis 1, 2, dan 3 ml. Penurunan kadar glukosa darah selama 180 menit pada uji antihiperglikemia sebagai berikut glibenklamid 10 mg> MSM 3 ml> MSM 2 ml> MSM 1 ml> KK 3 ml> KK 2 ml> KK 1 ml dan akuades. Pemberian minyak sawit merah dapat menurunkan kadar glukosa darah lebih cepat dibandingkan konsentrat karotenoid. Sifat antioksidatif komponen minor minyak sawit merah dapat menghambat oksidasi glukosa di dalam darah, sehingga pankreas dapat aktif memproduksi insulin secara normal. Oleh karena itu, minyak sawit merah dapat digunakan secara sinergis untuk membantu kerja obat hiperglikemia oral (OHO). Red Palm Oil is one of processed products from palm oil and also a food source rich in carotenoid,around500-800 ppm. Carotenoid concentrate is also a product obtained from the oil removal process and the carotenoid level increases to 6000-7000 ppm. This research aimed to determinethe potential of the red palm oil and the carotenoid concentrate as a preliminary test of anti-oxidant supplement Glucose Tolerance Test on Rat. The anti-oxidant activity of red palm oil and carotenoid concentrate was tested using DPPH method with 60 minutes incubation period. The anti-hyperglycemia activity evaluationwas conducted using animals(n=40) which divided into 8 groups: Negative control group (distilled water), positive control (glibenclamide 10 mg), three groups were given red palm oil with dose of1, 2, and 3 ml , and three groups were given carotenoid concentrate with dose of 1, 2, and 3 ml . The decrease of blood glucose for 180 minutes in anti-hyperglycemia test were in order ofglibenclamide10 mg> MSM 3 ml> MSM 2 ml> MSM 1 ml> KK 3 ml> KK 2 ml> KK 1 ml and distilled water. The red palm oil induction could lower blood glucose level faster than carotenoid concentrate, The anti-oxidative properties of minor componentsin red palm oil prevent the blood glucose oxidation and the pancreas could be actively produce insulin normally. In conclusion, red palm oil could be used synergistically toenhance the effectiveness oforal hyperglycemic agent.

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