Genetic and chemical characterization of avocado commercial cultivars avocado of Risaralda Colombia

Abstract This research aimed at performing the molecular characterization of commercial Papelillo avocado (Persea americana cv Lorena) cultivars from the municipality of Marsella (Risaralda, Colombia), as well as the physicochemical analysis and antioxidant activity assessment of the pulp and seed. An evaluation of 50 individuals among commercial varieties and possible patterns was performed using 17 microsatellite markers. Proximate analysis of the pulp was performed, and the fatty acid profile of oils, the antioxidant activity by the DPPH and FRAP methods, and the total phenolic content were evaluated. From the cluster analysis, Dice index, and Principal Coordinates Analysis, it became evident that all the individuals showed a tendency to group by populations. In addition, the pulp revealed high fiber contents (4.96–20.64%) and moisture (80.75–82.96%); however, it showed low oil content (5.97–6.56%). The fatty acid found in the highest proportion in seed oil is linoleic acid and that in pulp oil is oleic acid. The antioxidant activity by the DPPH method for seed oil (87.87 to 91.04%) presented a greater inhibition concerning to the pulp oil (20.34% and 24.43%), this same trend was observed by the FRAP method. Concerning the content of total phenols, the seed oil (31.94–76.30 mg GAE g-1) has a higher value than the pulp (30.18–54.30 mg GAE g-1). The set of samples was characterized as a significant source of genetic variability; thanks to the excellent alternatives they provide as rootstocks for commercial varieties such as the ‘Lorena’ cultivars. The chemical classification carried out in this study is of great importance, due to the lack of information about the oil of the ‘Papelillo’ avocado cultivated in different regions of Colombia.

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Evaluation of Chemical Characterization, Antioxidant Activity and Oxidative Stability of Some Waste Seed Oil

Turkish Journal of Agriculture - Food Science and Technology ◽

10.24925/turjaf.v5i1.48-53.909 ◽

2017 ◽

Vol 5 (1) ◽

pp. 48 ◽

Cited By ~ 1

Author(s):

Sibel Uluata ◽

Nurhayat Ozdemir

Keyword(s):

Antioxidant Activity ◽

Fatty Acid ◽

Linoleic Acid ◽

Oxidative Stability ◽

Seed Oil ◽

Lipid Hydroperoxide ◽

Chemical Characterization ◽

Thiobarbituric Acid ◽

Total Phenolic ◽

Thiobarbituric Acid Reactive Substance

In this study, fatty acid composition, antioxidant activity, total phenolic compounds (TPC) and oxidative stability of cherry seed (SCO), sweet cherry seed (SCSO), mulberry seed (MSO) and plum seed oil (PSO) were determined. Oleic acid was determined as primary fatty acid (42.9-67.3%), and followed by linoleic acid (23.4-41.8%) for SCO, SCSO and PSO. Linoleic acid was determined as primary fatty acid in MSO. γ-tocopherol was determined the main and highest tocopherol isomers varied from 579.9 to 605 mg/kg oil in SCO, SCSO and PSO, whereas δ-tocopherol was determined main tocopherol isomer with 1354mg/kg oil value in MSO. Plum seed oil (PSO) was the highest antioxidant activity values in both 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) assays. There was no significant differences in lipid hydroperoxide and TBARS (2-thiobarbituric acid-reactive substance) formation among SCO, SCSO and MSO. PSO had the highest induction period (15.1 h), followed by MSO (1.4 h), SCSO (1.5 h), SCO(1.3 h). PSO was oxidatively more stable than the other oil samples. This research shows that these waste seed oils have high antioxidant capacity and tocopherol content, so they could be used in food industry.

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Chemical characterization, fatty acid profile and antioxidant activity of Gustavia macarenensis fruit mesocarp and its oil from the Amazonian region of Ecuador as an unconventional source of vegetable oil

Grasas y Aceites ◽

10.3989/gya.0569181 ◽

2019 ◽

Vol 70 (2) ◽

pp. 298

Author(s):

J. J. Reyes Mera ◽

R. Abreu-Naranjo ◽

J. M. Alvarez-Suarez ◽

D. Viafara

Keyword(s):

Antioxidant Activity ◽

Fatty Acid ◽

Fatty Acid Profile ◽

High Performance ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Chemical Characterization ◽

Total Phenolic ◽

Linear Discriminant ◽

Chromatography Analysis

To our knowledge, this study is the first to report on the nutritional characterization, bioactive compounds and antioxidant activity of Amazonian G. macarenensis fruit. The fatty acid profile was determined using a high performance liquid chromatography analysis. The total phenolic content and antioxidant activity were determined using Folin Ciocalteu’s method and by radical scavenging activity, respectively. Moreover, a cluster analysis was carried out in order to classify the G. macarenensis fruit oil according to its fatty acid profile. Seven Fisher linear discriminant functions were obtained from the discriminant analysis. These models allow one to classify new fruits on the basis of their fatty acid profile. A high value for total lipids was obtained (53.57%). Its main components were palmitic and oleic acid. The TPC value (156.49 ± 2.62 mg GAE/Kg of oil) obtained from the G. macarenensis fruit pulp oil is higher than what was reported for some olive oil and Brazilian mango oil varieties.

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Productive and Qualitative Traits of Amaranthus Cruentus L.: An Unconventional Healthy Ingredient in Animal Feed

Animals ◽

10.3390/ani10081428 ◽

2020 ◽

Vol 10 (8) ◽

pp. 1428

Author(s):

Fabio Gresta ◽

Giorgia Meineri ◽

Marianna Oteri ◽

Carmelo Santonoceto ◽

Vittorio Lo Presti ◽

...

Keyword(s):

Antioxidant Activity ◽

Fatty Acid ◽

Total Phenolic Content ◽

Seed Oil ◽

Phenolic Content ◽

Animal Feed ◽

Total Phenolic ◽

Oil Composition ◽

Qualitative Traits ◽

Seed Oil Composition

Agronomic traits, oil content, fatty acid composition, antioxidant activity, and total phenolic content were studied on eight A. cruentus accessions cultivated in Southern Italy. A one-way ANOVA model was performed to compare accessions and the Principal Components Analysis was applied to identify patterns in our dataset and highlight similarities and differences. A. cruentus showed valuable seed yield (0.27 kg/m2, on average) comparable to the main tradition cereals used for animal feeding. Seed-oil composition showed significant differences among the accessions. Data showed a higher lipid content than most cereal grains (from 5.6 to 7.3%). Approximately 60% of fatty acids were unsaturated; linoleic fatty acid ranged from 19 to 34%, oleic acid from 29 to 36%, and alfa-linolenic fatty acid from 0.3 to 0.5%, respectively. The saturated/unsaturated fatty acid ratio ranged from 0.5 to 0.8, the hypocholesterolemic:hypercholesterolaemic ratio from 1.7 to 2.7, the Atherogenic Index from 0.38 to 0.66, the Thrombogenic Index from 0.85 to 1.48, the total phenolic content from 0.14 to 0.36 mg/g seeds, and the antioxidant activity (DPPH•) from 0.30 to 0.50. The studied seed-oil composition evidenced A. cruentus as a healthy ingredient for animal feed and consequently, as a possible substitute for traditional cereals. Accessions from Mexico and Arizona emerged for their high qualitative traits.

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Physico-chemical properties and fatty acid composition of Chrozophora tinctoria seeds as a new oil source

Grasas y Aceites ◽

10.3989/gya.0939182 ◽

2019 ◽

Vol 70 (4) ◽

pp. 328 ◽

Cited By ~ 3

Author(s):

S. Hazrati ◽

S. Nicola ◽

S. Khurizadeh ◽

A. Alirezalu ◽

H. Mohammadi

Keyword(s):

Fatty Acids ◽

Antioxidant Activity ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Acid Composition ◽

Seed Oil ◽

Chemical Properties ◽

Total Phenolic ◽

High Unsaturated Fatty Acid ◽

Physico Chemical

Chrozophora tinctoria L., usually known as dyer’s Croton, Turnsole or Giradol, has been used in various medicinal and food products for many years. However, no comprehensive research has been undertaken to assess its potential as a new seed oil crop. Therefore, the current study examined the fatty acid composition, physico-chemical properties and antioxidant activity of C. tinctoria seeds, grown in the southwest of Iran. The seed oil content was found to be 26.40%. The extracted oil was analyzed for fatty acid composition using gas chromatography (GC). The results showed that unsaturated fatty acids accounted for almost 91% of the total fatty acids. Linoleic acid was the dominant fatty acid (76.68%), followed by oleic acid (13.99%) and palmitic acid (5.32%). δ-tocopherol was the major tocopherol in the oil, representing 70 mg/100 g oil. The total phenolic content (151.70 mg GAE per 100 g oil) and total flavonoid content (1.17 mg QE oil) were also determined in the extracted oil. The antioxidant activity was measured by a DPPH assay and expressed as 45% of the seed oil. Due to its high oil yield and high unsaturated fatty acid content, C. tinctoria could be regarded as a new source of edible oil.

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Optimization of Natural Antioxidants Extraction from Pineapple Peel and Their Stabilization by Spray Drying

Foods ◽

10.3390/foods10061255 ◽

2021 ◽

Vol 10 (6) ◽

pp. 1255

Author(s):

Sofia C. Lourenço ◽

Débora A. Campos ◽

Ricardo Gómez-García ◽

Manuela Pintado ◽

M. Conceição Oliveira ◽

...

Keyword(s):

Antioxidant Activity ◽

Antioxidant Capacity ◽

Spray Drying ◽

Total Phenolic Content ◽

Phenolic Content ◽

Food Products ◽

Total Phenolic ◽

Dry Extract ◽

Pineapple Peel ◽

Dpph Method

Pineapple peel still contains an important amount of phenolic compounds and vitamins with valuable antioxidant activity. In this way, the aim of this study was the recovery of the bioactive compounds from pineapple peel using environmentally friendly and low-cost techniques, envisaging their application in food products. From the solid-liquid extraction conditions tested, the one delivering an extract with higher total phenolic content and antioxidant capacity was a single extraction step with a solvent-pineapple peel ratio of 1:1 (w/w) for 25 min at ambient temperature, using ethanol-water (80–20%) as a solvent. The resulting extract revealed a total phenolic content value of 11.10 ± 0.01 mg gallic acid equivalent (GAE)/g dry extract, antioxidant activity of 91.79 ± 1.98 µmol Trolox/g dry extract by the DPPH method, and 174.50 ± 9.98 µmol Trolox/g dry extract by the FRAP method. The antioxidant rich extract was subjected to stabilization by the spray drying process at 150 °C of inlet air temperature using maltodextrin (5% w/w) as an encapsulating agent. The results showed that the antioxidant capacity of the encapsulated compounds was maintained after encapsulation. The loaded microparticles obtained, which consist of a bioactive powder, present a great potential to be incorporated in food products or to produce bioactive packaging systems.

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Evaluation of phenolic compounds and antioxidant activity in some edible flowers

Open Agriculture ◽

10.1515/opag-2020-0087 ◽

2020 ◽

Vol 5 (1) ◽

pp. 857-870

Author(s):

Fernando Gonçalves ◽

João Carlos Gonçalves ◽

Ana Cristina Ferrão ◽

Paula Correia ◽

Raquel P. F. Guiné

Keyword(s):

Antioxidant Activity ◽

Total Phenolics ◽

Total Phenolic ◽

Potential Health ◽

Phenolic Contents ◽

Spectrophotometric Methods ◽

Potential Source ◽

Successive Extraction ◽

Dpph Method ◽

Total Anthocyanins

AbstractRecently, edible flowers (EF) have aroused increased interest because of their aesthetic properties as well as potential health benefits related to the occurrence of some bioactive compounds. Therefore, the aim of this work was to evaluate the total phenolics, anthocyanins, flavonoids, and antioxidant activity (AOA) (following DPPH and ABTS methods) in eleven EF. The samples were subjected to three successive extraction steps using methanol, and these extracts were then analysed for the aforementioned properties using spectrophotometric methods. The obtained extracts were used for the quantification of phenolic composition and AOA. The results indicated that, among the flowers analysed in this study, red rose, pink rose, and red carnation possessed the highest total phenolic contents (27.53, 23.30, and 18.17 mg g−1 gallic acid equivalents, respectively), total anthocyanins (3.07, 1.97, and 4.47 mg g−1 catechin equivalents [CE], respectively), and AOA (12.07, 15.77, and 12.93 mg g−1 TE, respectively, as given by the DPPH method or 8.23, 9.27 and 8.00 mg g−1 TE, respectively, as given by the ABTS method). The flowers with highest flavonoids contents were red carnation, Mexican marigold, and pink rose (17.50, 16.90, and 16.57 mg g−1 CE, respectively). Cluster analysis grouped the analysed flowers into two groups, those richest in phenolics with AOA and those not so rich. Finally, some important correlations were observed between the total phenolics and the AOA. In conclusion, these flowers could represent a potential source of natural compounds with antioxidant capacity.

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Analysis of Antioxidant and Antiallergic Active Components Extracted From the Edible Insect Oxya yezoensis

Natural Product Communications ◽

10.1177/1934578x211023363 ◽

2021 ◽

Vol 16 (5) ◽

pp. 1934578X2110233

Author(s):

Masahiro Saiki ◽

Naomichi Takemoto ◽

Maki Nagata ◽

Masako Matsumoto ◽

Yhiya Amen ◽

...

Keyword(s):

Antioxidant Activity ◽

Phenolic Compounds ◽

Unsaturated Fatty Acids ◽

Soluble Fraction ◽

Total Phenolic ◽

Key Factors ◽

Active Components ◽

Food And Agriculture ◽

Food And Agriculture Organization

In recent years, entomophagy has attracted increased attention, as it was recommended as a potential source of food by the Food and Agriculture Organization of the United Nations. In Japan, Oxya yezoensisis one of the most widely eaten insect species, but studies of its functionality as a food are limited. In this study, we reported the optimal characterization of the total phenolic compounds in methanolic extract (OME) and different fractions of OME. Additionally, the antioxidant and antiallergic activities of the OME fractions were evaluated. The results showed that the ethyl acetate-soluble fraction of OME has potential antioxidant activity, whereas the n-hexane-soluble fraction showed the strongest inhibition of β-hexosaminidase, which is one of the key factors in allergic reactions. It was concluded that phenolic compounds might contribute to the antioxidant activity while unsaturated fatty acids contribute to the antiallergy activity.

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Phytochemical screening and in vitro antibacterial, antifungal, antioxidant and antitumor activities of the red propolis Alagoas

Brazilian Journal of Biology ◽

10.1590/1519-6984.182959 ◽

2019 ◽

Vol 79 (3) ◽

pp. 452-459 ◽

Cited By ~ 15

Author(s):

F. R. G. Silva ◽

T. M. S. Matias ◽

L. I. O. Souza ◽

T. J. Matos-Rocha ◽

S. A. Fonseca ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activity ◽

Phenolic Compounds ◽

Gallic Acid ◽

Bioactive Metabolites ◽

Diffusion Method ◽

Total Phenolic ◽

Phytochemical Screening ◽

Dpph Method

Abstract The study aimed to evaluate the antimicrobial activity, antioxidant, toxicity and phytochemical screening of the Red Propolis Alagoas. Antimicrobial activity was evaluated by disk diffusion method. Determination of antioxidant activity was performed using the DPPH assay (1.1-diphenyl-2-picrylhydrazyl), FTC (ferric thiocyanate) and determination of phenolic compounds by Follin method. Toxicity was performed by the method of Artemia salina and cytotoxicity by MTT method. The phytochemical screening for the detection of allelochemicals was performed. The ethanol extract of propolis of Alagoas showed significant results for antimicrobial activity, and inhibitory activity for Staphylococcus aureus and Candida krusei. The antioxidant activity of the FTC method was 80% to 108.3% hydrogen peroxide kidnapping, the DPPH method showed an EC50 3.97 mg/mL, the content of total phenolic compounds was determined by calibration curve gallic acid, resulting from 0.0005 mg/100 g of gallic acid equivalent. The extract was non-toxic by A. salina method. The propolis extract showed high activity with a higher percentage than 75% inhibition of tumor cells OVCAR-8, SF-295 and HCT116. Chemical constituents were observed as flavonones, xanthones, flavonols, and Chalcones Auronas, Catechins and leucoanthocyanidins. It is concluded that the extract can be tested is considered a potential source of bioactive metabolites.

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Extraction and chemical characterization of Angelica sinensis polysaccharides and its antioxidant activity

Carbohydrate Polymers ◽

10.1016/j.carbpol.2013.02.007 ◽

2013 ◽

Vol 94 (2) ◽

pp. 731-736 ◽

Cited By ~ 33

Author(s):

Songtao Ai ◽

Xindong Fan ◽

Linfeng Fan ◽

Qi Sun ◽

Yu Liu ◽

...

Keyword(s):

Antioxidant Activity ◽

Chemical Characterization ◽

Angelica Sinensis

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NEW FLAVONOIDS FROM THE AERIAL PARTS OF POLYGONUM EQUISETIFORME SM (POLYGONACEAE)

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i2.15593 ◽

2017 ◽

Vol 9 (2) ◽

pp. 166 ◽

Cited By ~ 2

Author(s):

Sayed A. El-toumy ◽

Joslin Y. Salib ◽

Nabila H. Shafik ◽

Asmaa S. Abd Elkarim ◽

Gihan A. Mick

Keyword(s):

Antioxidant Activity ◽

Methyl Ester ◽

Secondary Metabolites ◽

Gallic Acid ◽

Total Phenolic ◽

Flavonoid Content ◽

Isolation And Identification ◽

Aerial Parts ◽

Dpph Method ◽

Phenolic And Flavonoid

Objective: The current study was to deal the isolation and identification of secondary metabolites from Polygonum equisetiforme and evaluation of antioxidant activity of its extract.Methods: The methanol-water extract (7:3) of the air-dried aerial parts of Polygonum equisetiforme was fractionated and separated to obtain the isolated compounds by different chromatographic techniques. Structures of these compounds were elucidated by UV and 1D⁄2D H⁄ C NMR spectroscopy and compared with the literature data. The crude extract was evaluated for in vitro antioxidant activity using the 2,2 diphenyl dipicryl hydrazine (DPPH) method.Results: Ten secondary metabolites were isolated from Polygonum equisetiforme in this study. Of which three new flavonoids named as 3,5,7,2’,5’ pentahydroxyflavone 3-O-b-D-glucopyranoside (1), 3,5,7,2’,5’ pentahydroxyflavone 3-O-b-D-glucopyranoside 8 C-sulphated (2) and quercetin 3-O-β-D-glucucorinde 6''-methyl ester 8-sulphated (3) as well as quercetin 3-O-β-D-glucucorinde methyl ester (4), quercetin 3-O-β-D-glucopyranoside (5), quercetin 7-O-β-D-glucopyranoside (6), quercetin(7), myricetin (8), P-methoxy gallic acid methyl ester (9) and gallic acid (10). The antioxidant potential of P. equisetiforme extract was evaluated by investigating it's total phenolic and flavonoid content and DPPH radical scavenging activity whereby the extract showed significant antioxidant activity (IC50 = 37.45 μg/ml). The total phenolic and flavonoid content was found to be 130.79±5.502 and 45.8±1.63 μg/ml, respectively.Conclusion: Polygonum equisetiforme is a promising medicinal plant, and our study tends to support the therapeutic value of this plant as an antioxidant drug.

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