scholarly journals Conservation of both hematocrit and liver regeneration in hepatectomies: a vascular occlusion approach in rats

2020 ◽  
Vol 33 (1) ◽  
Author(s):  
Eduardo Augustus MALINOWSKI ◽  
Jorge Eduardo Fouto MATIAS ◽  
Ana Paula PERCICOTE ◽  
Thaísa NAKADOMARI ◽  
Rogério ROBES ◽  
...  
Keyword(s):  
Liver Regeneration ◽  
Wistar Rats ◽  
Intervention Group ◽  
Vascular Occlusion ◽  
Perioperative Period ◽  
Control Group ◽  
Blood Products ◽  
Surgical Practice ◽  
Beneficial Effects ◽  
Occlusion Technique

ABSTRACT Background: Hepatectomies promote considerable amount of blood loss and the need to administrate blood products, which are directly linked to higher morbimortality rates. The blood-conserving hepatectomy (BCH) is a modification of the selective vascular occlusion technique. It could be a surgical maneuver in order to avoid or to reduce the blood products utilization in the perioperative period. Aim: To evaluate in rats the BCH effects on the hematocrit (HT) variation, hemoglobin serum concentration (HB), and on liver regeneration. Methods: Twelve Wistar rats were divided into two groups: control (n=6) and intervention (n=6). The ones in the control group had their livers partially removed according to the Higgins and Anderson technique, while the rats in the treatment group were submitted to BCH technique. HT and HB levels were measured at day D0, D1 and D7. The rate between the liver and rat weights was calculated in D0 and D7. Liver regeneration was quantitatively and qualitatively evaluated. Results: The HT and HB levels were lower in the control group as of D1 onwards, reaching an 18% gap at D7 (p=0.01 and p=0.008, respectively); BCH resulted in the preservation of HT and HB levels to the intervention group rats. BCH did not alter liver regeneration in rats. Conclusion: The BCH led to beneficial effects over the postoperative HT and serum HB levels with no setbacks to liver regeneration. These data are the necessary proof of evidence for translational research into the surgical practice.

Effect of Prunus Dulcis & Álpha-Tocopherol in Ethanol Induced Dyslipidemia In Wistar Rats

2020 ◽  
Vol 13 (4) ◽  
pp. 1619-1624
Author(s):  
Samuel Sundar Doss ◽  
J. Vijayakumar ◽  
E. Sukumar ◽  
K. Rekha
Keyword(s):  
Wistar Rats ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Prunus Dulcis ◽  
Alpha Tocopherol ◽  
Control Group ◽  
P Value ◽  
Low Density ◽  
Ethanol Group ◽  
Beneficial Effects

The study is aimed at assessing the effect of Prunus dulcis and alpha-tocopherol treatment against ethanol induced dyslipidemia in Wistar rats. 30 albino Wistar rats were selected based on the selection criteria and equally distributed into 5 groups – Control, ethanol, Prunus dulcis, alpha-tocopherol and combination of alpha-tocopherol + Prunus dulcis treated for 40 days. After the treatment for 40 days, all the animals were euthanized and a retro-orbital puncture was made to collect the blood samples for biochemical investigations. Obtained results were statistically analysed using ANOVA. Compared to ethanol group alpha tocopherol, Prunus dulcis and alpha tocopherol + Prunus dulcis treatment significantly decreased total cholesterol and triglycerides levels with p value <0.001. High density lipoprotein (66.31%) levels in the ethanol group were decreased compared to the control group and were significantly increased in other groups. Low density lipoprotein and Very low density lipoprotein levels were higher in the ethanol group compared with the control group and were significantly reduced in other groups with p value <0.001. Results suggest that ethanol has an ill effect on the lipid profile. Treatment with Prunus dulcius and alpha-tocopherol both solely or in combination has produced beneficial effects against dyslipidemia.

scholarly journals Oral L-Arginine lower neovascularization and the number of fibroblasts but failed to increase nitric oxide and epithelialization in the healing process of male white diabetic wistar rats (Rattus norvegicus)

2019 ◽  
Vol 3 (2) ◽  
pp. 24
Author(s):  
Fenni Liem ◽  
Wimpie Pangkahila ◽  
I Wayan Weta
Keyword(s):  
Nitric Oxide ◽  
Diabetes Mellitus ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Healing Process ◽  
Intervention Group ◽  
Control Group ◽  
White Rats ◽  
Nitric Oxide Level ◽  
The Mean

Introduction: Oral L-Arginine is a conditional essential amino acid that plays a role in wound healing in DM. The role of arginine in diabetic wounds is by enhancing blood circulation in the injured area and increasing oxygen supply to the wound tissue. The purpose of this study to prove the administration of oral L-Arginine toward vascularization status in wound healing of male white rats wistar diabetes mellitus. Methods:   A randomized posttest only control group study using with 36 diabetic induced wistar rats (Rattus Norvegicus) aged 2-3 months and weighing 180-200gram which then divided randomly into two groups. Nitric oxide level was measured on the third day and each group was then further divided into two groups for examination of neovascularization, fibroblasts and epithelialization on the seventh day and on the tenth day. Results: Administration oral L-Arginine failed to induce any significant change in Nitric Oxide level and wound gap closure. On the other hand, the results showed that the mean neovascularization was significantly different between the two groups on the 10th day (Control group vs intervention group: 4.22±1922 vs1.89±1364; p=0.009). In addition, the mean number of fibroblast at the 10th day was also significantly different (Control group vs intervention group: 74.11±28.57 vs 38.11±20.90; p=0.008). Conclusion: In conclusion, oral L-Arginine did not significantly affect nitric oxide and epithelialization while decreased neovascularization and the number of fibroblasts on day tenth in the healing process of male white rats diabetes mellitus

scholarly journals Pictorial information about subclinical atherosclerosis reduces the CVD risk: Results from the VIPVIZA RCT

2021 ◽  
Vol 28 (Supplement_1) ◽  
Author(s):  
A Bengtsson ◽  
M Norberg ◽  
N Ng ◽  
B Carlberg ◽  
C Gronlund ◽  
...  
Keyword(s):  
Risk Group ◽  
Subclinical Atherosclerosis ◽  
Intervention Group ◽  
Control Group ◽  
Intermediate Risk ◽  
Cvd Risk ◽  
Pictorial Information ◽  
Intervention Effect ◽  
Beneficial Effects

Abstract Funding Acknowledgements Type of funding sources: Public Institution(s). Main funding source(s): Region Västerbotten and the Swedish Research Council Background The VIPVIZA trial has previously shown beneficial effects on cardiovascular disease (CVD) risk scores 1 year after sending pictorial information of carotid ultrasound imaging (Picture 1) to individuals and their physicians Purpose To investigate whether the beneficial effects on CVD-risk observed at 1-year were sustained over three years  Methods VIPVIZA is a pragmatic prospective open-label randomized controlled trial with blinded evaluators performed within a CVD prevention programme integrated in the regular primary health care in Västerbotten County, Sweden. Individuals aged 40, 50 or 60 years old with one CVD risk factor were enrolled and randomised 1:1 to intervention (n = 1749, pictorial information about subclinical atherosclerosis provided to participants and physicians, Picture 1) or control group(n = 1783, no information to participants or physicians). Intervention participants also recieved a follow-up phone call and the corresponding physicians written guideline-based information about the clinical significance of carotid ultrasound results. Participants were examined at baseline (2013-2016), after one and at three years Results A significant beneficial effect on cardiovascular risk was observed at the 3-year follow-up; Framingham Risk Score (FRS) was 13.38 for the intervention group and 14.08 for the control group(p = 0.047) and SCORE was 1.69 vs. 1.82(p = 0.022) respectively. The 3-year results adjusted for sex and educational level showed significant differences between the intervention and control group in FRS, SCORE, P-Total-Cholesterol, P-LDL-Cholesterol and waist circumference in favour of the intervention group. Analysis by sex showed difference in differences(DID) in FRS for men -1.19(95% CI -2.01 to -0.37) and -0.50(95% CI -0.93 to -0.07) for women and in SCORE for men -0.20(95% CI -0.33 to -0.06) and -0.08 (95% CI -0.13 to -0.04) for women, between the two groups over 3 years. Similarly, there were significant differences in DID in all educational groups. Further stratification by baseline FRS and SCORE risk category, showed a beneficial pattern of the intervention in all risk groups, however the DID at the 3-year follow-up was statistically significant only in the intermediate risk group for both FRS -1.34(95% CI -2.13 to -0.56) and SCORE -0.19(95% CI -0.32 to -0.05) Conclusions This study provides evidence of sustained effects over three years of pictorial information of subclinical carotid atherosclerosis on the reduction of cardiovascular risk regardless of sex and educational level. Importantly, a statistically significant intervention effect was seen in the intermediate risk group, where the majority of CVD events occur. Visualization of subclinical atherosclerosis may be one way to approach individuals at intermediate risk of CVD, a group where sufficient prevention is often overlooked. However, further studies are needed to investigate the intervention effect on hard end points as CVD-events and death. Abstract Figure. Picture 1

scholarly journals The effect of daily walking exercise on sleep quality in healthy young adults

2020 ◽  
Author(s):  
Feifei Wang ◽  
Szilvia Boros
Keyword(s):  
Young Adults ◽  
Sleep Quality ◽  
Repeated Measures ◽  
Intervention Group ◽  
Control Group ◽  
Beneficial Effects ◽  
Walking Intervention ◽  
Walking Exercise ◽  
Group B ◽  
The Impact

Abstract Purpose Walking has beneficial effects on sleep quality in elderly population and patients in clinical settings. However, less is known whether walking improves sleep quality among healthy young adults. This study examined the effectiveness of a 12-week walking intervention on sleep quality among sedentary young adults. Methods Fifty-four healthy adults aged 19 to 36-years old participated a pedometer based aerobic walking intervention, a cross-over randomized control trial. Participants were assigned into two groups (group A and group B) randomly. The 12-week intervention was divided into three sessions equally. Sleep quality was assessed by Pittsburgh Sleep Quality Index (PSQI) before and after session 1 and session 3. Omron HJ-112 pedometer and daily walking diary facilitated the intervention process. Within group and between group comparisons were made for statistical analysis. Results Within group comparison by repeated measures showed that sleep duration (p < 0.01, F-test 22.79), sleep medication (p < 0.05, F-test 5.22), subjective sleep (p < 0.05, F-test 5.51) and global sleep quality (p < 0.01, F-test 12.19) were significantly improved. The comparison between intervention group and control group showed that sleep disturbance was significantly improved (p < 0.05). Conclusion Daily walking exercise has a significant effect on facilitating sleep quality and sleep components among young adults. Further studies are suggested to examine the impact of walking intensity on sleep quality.

scholarly journals Soymilk Formula Increases Estrogen and Reduces Testosterone Level in Male Infant White Wistar Rats

2019 ◽  
Vol 3 (1) ◽  
pp. 22 ◽  
Author(s):  
Eveline Margo ◽  
Wimpie Pangkahila ◽  
I Gusti Made Aman
Keyword(s):  
Testosterone Level ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Intervention Group ◽  
Male Infant ◽  
Cow Milk ◽  
Cow’S Milk ◽  
Control Group ◽  
Cow's Milk ◽  
Estrogen Level

Background: Children’s immunity system is relatively lower on first year of life, causing atopic babies, leading to allergy. Ideally, newborn babies are given breast milk as main food source on the first 6 months, but often being replaced with cow milk which can induce Cow’s Milk Protein Allergy. For the alternative can replace it with soymilk formula which contains phytoestrogen from isoflavon, which works just like estrogen. The aim of this study to determine whether administration of soymilk formula is capable on increasing estrogen level and reducing testosterone level on male infant white Wistar rats (Rattus norvegicus).Materials and Methods: This research used post test only control group design. Samples were consisted of 36 infant male white Wistar rats aged 7 days, weighing from 10-15 grams, divided into 2 groups, control group which was given cow’s milk and intervention group which was given soymilk. Each group were given intervention with the dose 5% of BW (grams), given twice-a-day orally for 21 days, and subsequently in the morning their blood samples were taken to examine the level of estrogen and testosterone hormone.Results: The study showed that the level of estrogen on intervention group was significantly higher than the control group with mean estrogen level of 0.55±0.03 pg/mL in the control group and 0.82±0.01 pg/mL in the intervention group, while the mean testosterone level was significantly lower on intervention group compared with control group (p<0.05) with 3.25±0.15 ng/mL on control group and 2.36±0.22 ng/mL on intervention group.Conclusion: Soymilk was able to increase estrogen level and reduce testosterone level on male infant white Wistar rats (Rattus norvegicus).Keywords: soymilk formula, estrogen, testosterone, male infant rats

scholarly journals The Effect of Intratesticular Injection of Human Adipose-Derived Stem Cell (hADSC) on Testicular Oxidative Stress and Spermatogenesis Process in the Varicocele Rat Model

2021 ◽  
Author(s):  
Safendra Siregar ◽  
Bambang Sasongko Noegroho ◽  
Ricky Adriansjah ◽  
Akhmad Mustafa ◽  
Ananta Bonar
Keyword(s):  
Oxidative Stress ◽  
Rat Model ◽  
Wistar Rats ◽  
Histopathological Examination ◽  
Therapy Group ◽  
Intervention Group ◽  
Testicular Tissue ◽  
Control Group ◽  
Group I ◽  
Group Ii

Abstract Introduction: Varicocele is the predominant cause of male infertility and was found in 19% - 41% of men with primary infertility and 45% - 81% of men with secondary infertility. Human adipose Derived Stem Cells (hADSC) can suppress oxidative stress in some oxidative injury model. Therefore, this study would like to investigate the effect of intratesticular hADSC injection on MDA level and spermatogenesis process by histopathological examination in the varicocele rat model.Method: This is an experimental study. A total sampling of 9 male Wistar rats were divided into three groups. Group I consist of 1 Wistar rats without any treatment or model (sham group), group II consist of 4 Wistar rats with varicocele model without hADSC therapy (control group), and group III consist of 4 Wistar rats with varicocele model and were given injections of 1.0x106 hADSC cells intratesticularly 30 days after model was made (therapy group). Testicular tissue was harvested for evaluation. Results: In all varicocele model rats (group II and III), the result of MDA level in therapy group (2.53 mol/liter) was significantly lower than the MDA level in control group (4.43 mol/liter) (p = 0.01). On histopathological examination, the average Johnson's Score in the therapy and control group was 9,77 and 9,18, respectively. The analysis showed Johnson’s score in the intervention group was significantly higher (p = 0.018). Conclusion: Intratesticular injection of hADSC can help reduce MDA levels and improve spermatogenesis process, which is damaged by varicoceles.

scholarly journals Coconut Oil Promotes Greater Satiety and Reduces Blood Cholesterol, But Induces Obesity, Anxiety and Impaired Bone Formation in Adult Wistar Rats

2021 ◽  
Author(s):  
Ítalo Gomes Reis ◽  
Arthur Rocha-Gomes ◽  
Alexandre Alves da Silva ◽  
Mayara Rodrigues Lessa ◽  
Nísia Andrade Villela Dessimoni Pinto ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Caloric Intake ◽  
Coconut Oil ◽  
Blood Cholesterol ◽  
Control Group ◽  
Virgin Coconut Oil ◽  
Total Blood ◽  
Beneficial Effects ◽  
To Receive ◽  
Tibia Length

Background: The aim of this study was to evaluate the nutritional effects of supplementation with virgin coconut oil (VCO) in Wistar rats over a sub-chronic period (6 weeks).Methods: Twelve Wistar rats were used and randomly assigned to receive (n = 6): control - lab chow; coconut oil (CO) - lab chow with added virgin coconut oil (20%). Food and caloric intake, weight gain, food efficiency, body mass index, femur and tibia length, bone mineral composition and blood biochemistry were evaluated.Result: The CO group showed an energy intake closed to control group. Also, the supplementation with VCO generated a decrease in total blood cholesterol as compared to the control group. However, the CO group showed accumulation of fat mass, shorter femur length and anxiogenesis in relation to the control group. These results indicate few beneficial effects from the sub-chronic use of VCO and indicate that its consumption in large quantities for long periods should be questioned.

scholarly journals Study on Psychological Influence of Nursing Intervention on Orthopedic Operation Patients during Perioperative Period

2015 ◽  
Vol 4 (2) ◽  
pp. 12
Author(s):  
Zeng Rong
Keyword(s):  
Intervention Group ◽  
Perioperative Period ◽  
Nursing Intervention ◽  
Psychological State ◽  
Control Group ◽  
Psychological Reactions ◽  
Psychological Reaction ◽  
Orthopedic Operation ◽  
Random Number Table ◽  
Psychological Influence

<p><strong>Objective</strong>: to discuss psychological influence of orthopedic operation patients with nursing intervention during perioperative period. <strong>Methods</strong>: 106 cases of orthopedic operation patients from August 2014 to March 2015 in our hospital were selected. According to the random number table, all cases were divided into two groups with principle of informed consent. The control group: 53 cases were treated with the conventional nursing; the intervention group: 53 cases were treated with the nursing intervention. Psychological reactions were compared in the two groups. <strong>Results</strong>: After nursing, the intervention group had the good rate of psychological reaction 86.8%, which was much higher than that in the control group (66.0%). Different results between the two groups were significant in Statistics (<em>p</em> &lt; 0.05); however, the intervention group had the rate of pain occurrence 54.7%, which was much lower than that in the control group (73.6%). Different results between the two groups were significant in Statistics (<em>p</em> &lt; 0.05). <strong>Conclusion</strong>: Nursing intervention could obviously improve psychological state of patients, reduce pain occurrence and accelerate recovery.</p>

Alterations in the biochemical parameters and the spermatic function generated by obesity in rats

2019 ◽  
Vol 8 (5) ◽  
pp. 193-201
Author(s):  
Zahira Abbes ◽  
Demmouche A. ◽  
Menadi N. ◽  
Mai A. H. ◽  
Bahri M. R. ◽  
...  
Keyword(s):  
Microscopic Examination ◽  
Wistar Rats ◽  
Intervention Group ◽  
Diet Group ◽  
Control Group ◽  
Standard Diet ◽  
Testicular Weight ◽  
Consistent Change ◽  
Hyperlipidic Diet ◽  
Testicular Hypertrophy

The aim of the present study was to assess the effects of a hyperlipidic diet set before puberty in male Wistar rats’ gonadal weights and testicular func-tions. Males rats were used for the study, they were randomly distributed into 2 groups: Control Group (CG: standard diet (normolipidemic) and the second: Intervention Group (IG: hyperlipidemic diet), after 7 days of experi-mentation, 3 rats were sacrificed per week, blood samples were collected and level of HDL, LDL and triglyceride were analyzed. A significant reduction (p<0.05) in testicular weight in the control group was observed compared with the hyperlipidic diet group, triglyceride levels showed a consistent change over the weeks of the study, HDL levels showed a consistent change during the 5 weeks of the study, Photomicraphie of the testicles of Wistar rats in the hyperlipid diet group for the first week showed Sertoli cell hyper-plasia ,during the second week microscopic examination showed significant testicular hypertrophy the microscopic examination during the fifth week showed hyperplasia of the seminal vesicle characterized by an increase in the number of glandular epithelial cells. The proliferating epithelium may form papillary structures with supporting stroma and with extension into the glandular lumen and total absence of sperm cells. Obesity is associated with many metabolic abnormalities. It has been found that these metabolic ab-normalities induce disorders of spermatogenesis. Our results show that the hyperlipidic diet affects the gonads significantly with hypertrophic testes, the presence of hyperplastic seminiferous tubes, as well as a fine basement membrane.

scholarly journals A Pedometer-Based Physically Active Learning Intervention: The Importance of Using Preintervention Physical Activity Categories to Assess Effectiveness

2019 ◽  
Vol 31 (3) ◽  
pp. 356-362 ◽  
Author(s):  
Jade L. Morris ◽  
Andy Daly-Smith ◽  
Margaret A. Defeyter ◽  
Jim McKenna ◽  
Steve Zwolinsky ◽  
...  
Keyword(s):  
Physical Activity ◽  
Active Learning ◽  
Sedentary Time ◽  
Vigorous Physical Activity ◽  
Intervention Group ◽  
Primary School Children ◽  
Control Group ◽  
Physically Active ◽  
Beneficial Effects ◽  
Learning Intervention

Purpose: To assess physical activity outcomes of a pedometer-based physically active learning (PAL) intervention in primary school children. Methods: Six paired schools were randomly allocated to either a 6-week teacher-led pedometer-based physically active learning intervention or a control (n = 154, female = 60%, age = 9.9 [0.3] y). Accelerometers assessed total daily sedentary time, light physical activity (LPA), and moderate-to-vigorous physical activity (MVPA). Preintervention mean daily MVPA minutes grouped participants as Low Active (<45 min/d) and High Active (≥45 min/d). Results: From the final sample size, the intervention (n = 52) significantly improved LPA versus control (n = 31, P = .04), by reducing sedentary time. More intervention (+10%) than control (+3%) pupils met the 60 minutes per day guidelines. In both intervention subgroups, pupils spent less time in LPA (P < .05) versus control. The greatest nonsignificant increase was found in the Low Active pupils MVPA levels. Conclusions: Improvements in LPA were statistically significant in the intervention versus control group. In subgroup analysis, Low Active pupils in the intervention showed the greatest beneficial effects and the Most Active pupils may have replaced MVPA and sedentary time with LPA. The intervention group housed clusters of pupils showing variable responsiveness, justifying routine examination of subgroup variability in future studies.

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