Alterations in the biochemical parameters and the spermatic function generated by obesity in rats

2019 ◽  
Vol 8 (5) ◽  
pp. 193-201
Author(s):  
Zahira Abbes ◽  
Demmouche A. ◽  
Menadi N. ◽  
Mai A. H. ◽  
Bahri M. R. ◽  
...  
Keyword(s):  
Microscopic Examination ◽  
Wistar Rats ◽  
Intervention Group ◽  
Diet Group ◽  
Control Group ◽  
Standard Diet ◽  
Testicular Weight ◽  
Consistent Change ◽  
Hyperlipidic Diet ◽  
Testicular Hypertrophy

The aim of the present study was to assess the effects of a hyperlipidic diet set before puberty in male Wistar rats’ gonadal weights and testicular func-tions. Males rats were used for the study, they were randomly distributed into 2 groups: Control Group (CG: standard diet (normolipidemic) and the second: Intervention Group (IG: hyperlipidemic diet), after 7 days of experi-mentation, 3 rats were sacrificed per week, blood samples were collected and level of HDL, LDL and triglyceride were analyzed. A significant reduction (p<0.05) in testicular weight in the control group was observed compared with the hyperlipidic diet group, triglyceride levels showed a consistent change over the weeks of the study, HDL levels showed a consistent change during the 5 weeks of the study, Photomicraphie of the testicles of Wistar rats in the hyperlipid diet group for the first week showed Sertoli cell hyper-plasia ,during the second week microscopic examination showed significant testicular hypertrophy the microscopic examination during the fifth week showed hyperplasia of the seminal vesicle characterized by an increase in the number of glandular epithelial cells. The proliferating epithelium may form papillary structures with supporting stroma and with extension into the glandular lumen and total absence of sperm cells. Obesity is associated with many metabolic abnormalities. It has been found that these metabolic ab-normalities induce disorders of spermatogenesis. Our results show that the hyperlipidic diet affects the gonads significantly with hypertrophic testes, the presence of hyperplastic seminiferous tubes, as well as a fine basement membrane.

scholarly journals The effect of oat bran consumption on gestational diabetes: a randomized controlled clinical trial

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Zahra Barati ◽  
Mina Iravani ◽  
Majid Karandish ◽  
Mohammad Hosein Haghighizadeh ◽  
Sara Masihi
Keyword(s):  
Clinical Trial ◽  
Blood Glucose ◽  
Gestational Diabetes ◽  
Fasting Blood Glucose ◽  
Intervention Group ◽  
Control Group ◽  
Controlled Clinical Trial ◽  
Standard Diet ◽  
Oat Bran ◽  
Significant Difference

Abstract Background Gestational diabetes is the most common medical complication in pregnancy, and it has many side effects for the mother and the fetus. The aim of this study was to evaluate the effect of oat bran consumption on gestational diabetes. Methods This study is a randomized clinical trial that was performed on 112 women with gestational diabetes treated with diet. Participants were randomly divided into two groups of 56. Participants in both groups were given a diet for gestational diabetes. In addition to the diet, the intervention group received 30 g of oat bran daily for 4 weeks at lunch and dinner. Tests of fasting blood glucose and two-hour postprandial (2hpp) glucose were taken from both groups: before the intervention, and 2 and 4 weeks after the start of the intervention. Data analysis was performed using SPSS statistical software (version 22) using independent t-test, as well as Chi-square and Mann-Whitney tests. P values less than 0.05 were considered statistically significant. Results There was no statistically significant difference between the two groups in terms of mean blood glucose before the intervention, while 2 and 4 weeks after the intervention, mean fasting blood glucose and two-hour postprandial (2hpp) glucose decreased significantly in the intervention group compared with the control group (P < 0.001). Conclusion Based on the results of this study, the addition of oat bran to the standard diet for pregnant women with gestational diabetes reduced fasting blood glucose and two-hour postprandial (2hpp) glucose. More detailed studies with higher sample sizes are recommended to prove the effectiveness of this valuable dietary supplement. Trial registration IRCT registration number:IRCT20191220045828N1. Registration date: 2020-04-18. Registered while recruiting.

scholarly journals A Calorie-Restricted Ketogenic Diet Reduces Cerebral Cortex Vascularization in Prepubertal Rats

Nutrients ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 2681
Author(s):  
Viggiano ◽  
Meccariello ◽  
Santoro ◽  
Secondulfo ◽  
Operto ◽  
...  
Keyword(s):  
Cerebral Cortex ◽  
Substantia Nigra ◽  
Ketogenic Diet ◽  
Capillary Density ◽  
Diet Group ◽  
Cerebral White Matter ◽  
Control Group ◽  
Standard Diet ◽  
Ketogenic Diets ◽  
Prepubertal Rats

The antiepileptic effect of ketogenic diets is acknowledged but its mechanism of action is poorly understood. The present work aimed to evaluate possible effects of a calorie-restricted ketogenic diet (CRKD) on brain growth and angiogenesis in normal prepubertal rats. Two groups of prepubertal rats were fed with a standard diet (group 1) or a CRKD (group 2) for ten weeks. Then, rats were sacrificed and the thickness for the following structures was evaluated by histology: (1) cerebral cortex, (2) deep cerebral white matter, and (3) substantia nigra. The capillary density was also evaluated within: (1) cerebral cortex, (2) dentate gyrus of the hippocampus, (3) periaqueductal grey matter, and (4) substantia nigra. The results showed a smaller thickness of all the areas examined and a reduced capillary density within the cerebral cortex in the CRKD-treated group compared to the control group. These findings suggest an association between reduced angiogenesis within the cerebral cortex and the antiepileptic effects of CRKD.

scholarly journals The effect of vitamin C on Endosulfan-induced oxidative stress parameters in prepubertal rats

Biomedicine ◽  
2020 ◽  
Vol 39 (2) ◽  
pp. 333-338
Author(s):  
Kalaivani Manokaran ◽  
Vasanthalaxmi Krishnananda Rao ◽  
Nilima . ◽  
Manjula Shimoga Durgoji Rao ◽  
Sucheta Prasanna Kumar
Keyword(s):  
Oxidative Stress ◽  
Vitamin C ◽  
Wistar Rats ◽  
Statistical Significance ◽  
Treated Group ◽  
Reproductive Organs ◽  
Protective Role ◽  
Control Group ◽  
Group I ◽  
Testicular Weight

Introduction and Aim: Oxidative stress plays a very important role in endosulfan-induced toxic effects on reproductive organs. Vitamin C is a potent antioxidant which plays an important role in decreasing oxidative stress. The present study was aimed to investigate the protective role of vitamin C against endosulfan-induced testicular toxicity in Wistar rats. To investigate a protective effect of vitamin C against endosulfan induced toxicity on biochemical changes. Materials and Methods: Seventy male neonatal Wistar rats were divided into  seven groups. The group  I was taken as the control group, the endosulfan-treated were grouped into II (3 mg/kg body weight (BW) and group III (6 mg/kg BW), Group IV (9 mg/kg BW) and Group V (12 mg/kg BW). Group VI (9 mg/kg BW) and group VII (12 mg/kg BW) were pretreated with vitamin C (20 mg/kg BW) for 60 days. After  the experimental procedures, the testicular weight, lactate dehydrogenase (LDH) enzyme and testosterone in plasma, LDH, steroidogenic enzymes 3?-HSD and 17?-HSD in testis were evaluated. One-way ANOVA was used to determine the statistical significance. Results: Significant improvement in the testicular weight (P<0.05) , LDH (P<0.05) levels both in plasma and testis, increase in testosterone(P<0.001) and steroidogenic enzyme levels(P<0.001) was observed in the group pretreated with vitamin C treated group when compared to the endosulfan treated group. Conclusion: Vitamin C decreases the toxic effect of endosulfan on testis. The present action might be  due to its antioxidative properties.

scholarly journals Evaluation of Rat Muscle Structure in Pre-Menopausal Period after Regular Resistance Exercise and Taking Vitamin D and Calcium Supplements

2020 ◽  
Author(s):  
Seyedeh Zahra Hosseini Sisi ◽  
Mohammad Ali Azarbayjani ◽  
Mohammad Reza Vafaeenasab ◽  
Maghsoud Peeri ◽  
Mohammad Reza Dehghani
Keyword(s):  
Vitamin D ◽  
Resistance Training ◽  
Resistance Exercise ◽  
Muscle Fiber ◽  
Wistar Rats ◽  
Fiber Diameter ◽  
Inflammatory Cells ◽  
Control Group ◽  
Standard Diet ◽  
Calcium Supplements

Introduction: Menopause can change the musculoskeletal system by reducing estrogen hormone levels. The aim of this study was to evaluate the effect of regular resistance training accompanied by vitamins D and calcium supplements during pre-menopausal period on muscle tissues of Wistar rats’ postmenopausal period. Methods: In this experimental study, female Wistar rats were randomly divided into control, placebo, vitamins D, Calcium, excercise, vitamins D- Calcium, vitamins.D- excercise, Calcium - excercise, and vitamins.D‐ Calcium - excercise groups. Control and placebo groups were fed with a standard diet and sesame oil, respectively. The amount of calcium consumed was 35 mg / kg and the amount of vitamin D was 10000 IU during the two-month period, and in the resistance training groups, two months of training was performed with a ladder. Then ovariectomy was done and sample of the gastrocnemius muscle was taken and the diameter of muscle fiber, the number of muscle and adipocyte cell as well as the number of inflammatory cells were studied. The statistical analysis by means of one-way ANOVA and SPSS version 20 was performed. Results: Compared to the control group, the increase in the number of adipocyte cells were observed in the vitamin D, calcium-vitamin D, vitamin D-resistance exercise and placebo groups but it was not significantly different. Muscle fiber diameter significantly increased in all groups compared to the control group (P <0.001). The number of muscle cells increased significantly in the calcium-resistance exercise group (103± 9), vitamin D-resistance exercise (105 ±6.9) and calcium-vitamin D-resistance exercise (114 ± 3.16) compared to the control group, while, there was a significant increase in inflammatory cells only in the control and placebo groups (p≤0.05). Conclusion: Regular resistance exercise accompanied with vitamin D and Calcium supplements in premenopausal period can improve muscle fiber diameter and muscle cell numbers; in addition, the process of decrease in the muscle strength and muscle inflammation during menopause will be delayed.

scholarly journals Effect of Solanum gilo leaf extract on some haematological indices of albino Wistar rats

2021 ◽  
Vol 12 (1) ◽  
pp. 108-111
Author(s):  
Nsimah Monday Essien ◽  
Justina Nwadimma Nwangwa ◽  
Charles Cyprian Mfem ◽  
Johnbull Martins Uket ◽  
Efiok Aniekan Archibong
Keyword(s):  
Blood Cell ◽  
Wistar Rats ◽  
Blood Parameters ◽  
Diet Group ◽  
Control Group ◽  
Carbohydrate Consumption ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Haematological Indices

Solanum gilo (eggplant) is an edible vegetable whose leaf is reported to possess numerous medicinal properties due to its rich constituents. Against this backdrop, there is possibility that its consumption may have effect on haematological indices. Therefore this research sought to assess the effect of Solanum gilo leaf diet on some haemotological indices of albino wistar rats. About twenty four (24) albino wistar rats weighing between 120-150mg were randomly assigned into four groups of six animals each. Each rat was allowed to drinking water ad labitum. Control group received normal rodent chow while group 2 was fed 5% Solanum gilo diet. Group 3 was fed 10% Solanum gilo diet and group 4 received 20% Solanum gilo diet. The different diets were prepared by mixing different proportions of ground S. gilo leaves with rodent chow. Automated blood cell analyzer (Model PCE 210, Japan) was used for analysis of haematological parameters. Preliminary study on its proximate composition, showed the appreciable presence of crude fat, protein, fibre and carbohydrate. Consumption of S. gilo caused significant improvement in some blood parameters; notably packed cell volume (PCV), white blood cell (WBC) and platelet counts. Hence, eggplant is a nutritious vegetable with healing, the therapeutic and immunity properties. If exploited in human diet has the potentials of improving quality of life.

scholarly journals Antioxidant and Hepatoprotective Activity of a New Tablets Formulation fromTamarindus indicaL.

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Jesús Rafael Rodriguez Amado ◽  
Ariadna Lafourcade Prada ◽  
Julio Cesar Escalona Arranz ◽  
Renato Pérez Rosés ◽  
Humberto Morris Quevedo ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Serum Levels ◽  
Hepatoprotective Activity ◽  
Negative Control ◽  
Diet Group ◽  
Control Group ◽  
Standard Diet ◽  
Tamarindus Indica ◽  
Sprague Dawley ◽  
Group I

Hepatotoxic chemicals damage liver cells primarily by producing reactive oxygen species. The decoction of the leaves ofTamarindus indicaL. is used for liver disorders. In this work we evaluated the hepatoprotective activity of a tablet formulation of this plant. Thirty-five Sprague Dawley rats were randomly divided into five groups (n=7). First group (I) is control group, fed with standard diet. Groups II to V (hepatotoxic groups) were subjected to a subcutaneous injection of CCl4(0.5 mL/kg). Group II was negative control, fed with standard diet; group III was subjected to administration of Silymarin 150 mg/kg and groups IV and V were treated with tablets in dose of 100 mg/kg and 200 mg/kg, respectively. Lipid peroxidation and the activity of superoxide dismutase, catalase, and reduced glutathione were evaluated. Serum levels of alanine aminotransferase, aspartate aminotransferase, gamma-glutamine transferase, alkaline phosphatase, and a lipid profile were evaluated too. The tablets inhibit lipid peroxidation. The redox balance (SOD-CAT-GSH) remains normal in the experimental groups treated with tablets. The liver function using dose of 200 mg/kg of tablets was better than the other experimental groups. These results justify, scientifically, the ethnobotanical use of the leaves ofTamarindus indicaL.

scholarly journals Effect of Kefir, Yacon (Smallanthus sonchifolius) and the Association Between Yacon and Kefir on Intestinal Physiology in Rats With Colon Cancer

2021 ◽  
Vol 5 (Supplement_2) ◽  
pp. 309-309
Author(s):  
Neuza Costa ◽  
Keila Zanardi ◽  
Caroline Woelffel ◽  
Andre Costa ◽  
Mirelle Viana ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Intestinal Permeability ◽  
Wistar Rats ◽  
Short Chain Fatty Acids ◽  
Diet Group ◽  
Standard Diet ◽  
Smallanthus Sonchifolius ◽  
Intestinal Physiology ◽  
Significant Difference ◽  
Male Wistar Rats

Abstract Objectives To evaluate the effect of consumption of yacon flour, kefir and the association between them on colon cancer induced by 1,2-dimethylhydrazine (DMH) in male Wistar rats, SCFA production, fecal pH and intestinal permeability. Methods The study was conducted with 60 adult Wistar rats divided into 5 groups. For 5 weeks, groups T, Y, K and YK received 1,2-dimethylhydrazine (DMH) to induce colon cancer. After 5 weeks of DMH administration, animals in groups C and T received the standard diet, group Y received a diet with yacon flour with 5% FOS, group K received 1mL /day of kefir and the YK group received a diet with yacon and kefir, for 15 weeks. After euthanasia, intestinal lesions, intraluminal pH, short-chain fatty acids (SCFA) and intestinal permeability were analyzed. Results An increase in macroscopic lesions was observed in groups K (58%) and YK (42%) and a reduction of 5% in group Y, compared to group T. In addition, an increase in neoplastic changes was observed in all groups compared to group T: Y (33%), K (67%) and YK (78%). There was no significant difference in the concentrations of acetate and proprionate, pH, lactulose and mannitol between groups, and butyrate was not found in the samples. Conclusions The consumption of yacon flour, kefir and their association did not influence intestinal physiology and promoted the worsening of the development of colon carcinogenesis in rats. Funding Sources FAPES - Fundação de Amparo à Pesquisa e Inovação do Espírito Santo CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico

scholarly journals THE EFFECTS OF CORN FLOUR WITH TEMPEH FLOUR SUPPLEMENTATION FEEDING IN DIABETES MELLITUS WISTAR RATS TOWARD BLOOD GLUCOSE LEVEL

2017 ◽  
Vol 4 (2) ◽  
pp. 24
Author(s):  
Fatifa Asmarani ◽  
Bambang Wirjatmadi ◽  
Merryana Adriani
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Blood Sugar ◽  
Wistar Rats ◽  
Control Group ◽  
Standard Diet ◽  
Corn Flour ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Sugar Levels

Background: Diabetes mellitus (DM) is metabolic disorder syndrome characterized by hyperglycemia due to deficiency of insulin secretion. Patients of diabetic patients increases each year, so it needs proper handling. Corn flour is a source of carbohydrates with a low glycemic index to help reduce the rise in blood sugar levels. In addition, supplementation with tempeh flour containing isoflavones act to protect cells from free radical thereby inhibiting damage cells. Fiber in tempeh affect blood glucose levels because it slows the absorption of glucose.Objective: To determine the effect supplementation of corn flour with tempeh flour on blood sugar levels in diabetes mellitus Wistar rats.Methods: The study was true experimental design with Pre-posttest control group design, samples were 20 male Wistar rats aged 2-3 months were then divided into 4 groups (T0, T1, T2, T3.) T0 was injected with alloxan as 30 mg / 150 grams of BB rats are subsequently treated with standard diet; T1 was dministered by standard diet 50% + 50% corn flour; T2 was administered by standard diet 50% + 30% + corn flour tempeh flour 20%; and T3 was administered by standard diet of corn flour 50% + 15% + 35% tempeh flour for 2 weeks. Blood glucose levels were measured with a glucose kit (GOD FS). Research data were analyzed by One Way ANOVA followed by Tukey's Post Hoc test p-value <0.05.Results: There were significant differences on blood sugar levels in all groups (p = 0.000): control group (only dietary standards), T1 (administered by standard diet 50% + corn flour 50%), T2 (administered by a standard diet 50% + cornstarch 30% tempeh flour + 20%) and T3 (administered by standard diet of corn flour 50% + 15% + 35% tempeh flour).Conclusion: corn flour with tempeh flour supplementation effect on decresing of blood sugar levels.

scholarly journals Growth rate and acid-base balance in turkeys fed a silage-containing diet modified by different dietary cation-anion difference

2017 ◽  
Vol 73 (12) ◽  
pp. 786-791
Author(s):  
Kamil Gruszczyński ◽  
Wacław Strobel ◽  
Marta Wójcik ◽  
Urszula Kosior-Korzecka ◽  
Joanna Wessely-Szponder ◽  
...  
Keyword(s):  
Body Weight ◽  
Body Weight Gain ◽  
Diet Group ◽  
Control Group ◽  
Standard Diet ◽  
Acid Base Balance ◽  
Acid Base ◽  
Base Balance ◽  
Group B ◽  
Group D

The aim of the study was to find the responses of acid-base parameters and performance parameters of turkeys to a corn silage (CS) diet with different values of the dietary cation-anion difference (DCAD). The turkeys were fed as follows: group A (control) – standard diet (SD) (60%) plus CS (40%); group B – SD (60%), CS (40%) plus 240 g of CaCl2 per100 kg of diet; group C – SD (60%), CS (40%) plus 480 g of CaCl2 per 100 kg of diet; group D – SD (60%), CS (40%) plus 240 g of NaHCO3 per 100 kg of diet; group E – SD (60%), CS (40%) plus 480 g NaHCO3 per 100 kg of diet. The addition of the smaller amount of CaCl2 reduced DCAD, which ranged between 49.75 ± 6.29 mEq/kg DM and 93.56 ± 3.34 mEq/kg DM. An increased content of CaCl2 led to high, negative values of DCAD. NaHCO3 supplemented in both doses resulted in a significant elevation of DCAD. The addition of CS to the SD led to a lower body weight in comparison to that in the B, C, D and E groups. Forage acidification and alkalization improves body weight gain (BWG) at every stage of feeding. Compared to the control group, the anion gap was insignificantly lower in birds exposed to the acidic diet, and was comparable to the decrease in HCO3 –. Conversely, the addition of NaHCO3 to the diet led to a marked elevation in HCO3 – to 29.63 ± 0.4 mEq/L in group D and to 30.3 ± 0.69 mEq/l in group E. In conclusion, a change in DCAD during the feeding of forage containing CS exerts stimulatory effects on productive parameters of turkeys....

scholarly journals Oral L-Arginine lower neovascularization and the number of fibroblasts but failed to increase nitric oxide and epithelialization in the healing process of male white diabetic wistar rats (Rattus norvegicus)

2019 ◽  
Vol 3 (2) ◽  
pp. 24
Author(s):  
Fenni Liem ◽  
Wimpie Pangkahila ◽  
I Wayan Weta
Keyword(s):  
Nitric Oxide ◽  
Diabetes Mellitus ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Healing Process ◽  
Intervention Group ◽  
Control Group ◽  
White Rats ◽  
Nitric Oxide Level ◽  
The Mean

Introduction: Oral L-Arginine is a conditional essential amino acid that plays a role in wound healing in DM. The role of arginine in diabetic wounds is by enhancing blood circulation in the injured area and increasing oxygen supply to the wound tissue. The purpose of this study to prove the administration of oral L-Arginine toward vascularization status in wound healing of male white rats wistar diabetes mellitus. Methods:   A randomized posttest only control group study using with 36 diabetic induced wistar rats (Rattus Norvegicus) aged 2-3 months and weighing 180-200gram which then divided randomly into two groups. Nitric oxide level was measured on the third day and each group was then further divided into two groups for examination of neovascularization, fibroblasts and epithelialization on the seventh day and on the tenth day. Results: Administration oral L-Arginine failed to induce any significant change in Nitric Oxide level and wound gap closure. On the other hand, the results showed that the mean neovascularization was significantly different between the two groups on the 10th day (Control group vs intervention group: 4.22±1922 vs1.89±1364; p=0.009). In addition, the mean number of fibroblast at the 10th day was also significantly different (Control group vs intervention group: 74.11±28.57 vs 38.11±20.90; p=0.008). Conclusion: In conclusion, oral L-Arginine did not significantly affect nitric oxide and epithelialization while decreased neovascularization and the number of fibroblasts on day tenth in the healing process of male white rats diabetes mellitus

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