Endodontics pastes formulated with copaiba oil: action on oral microbiota and dentin bridge formation in dogs

This study analysed the effect of pastes formulated with calcium hydroxide P.A. and different vehicles (saline solution - paste A and Copaifera langsdorffii Desfon oil - paste B) on oral microorganisms and dentin bridge formation in dogs. The antimicrobial action of the pastes and their components was analysed by the minimum inhibitory concentration in agar gel technique. The components were diluted and tested on fifteen standard strains of microorganisms associated with endodontic diseases. The microorganisms were cultivated and after incubation data was analysed using One-Way ANOVA and Turkey's test (P≤0.05). Four superior incisors of ten animals were used to evaluate dentin bridge formation. Two incisors were capped with paste A (GA) and two with paste B (GB). After 90 days, the teeth were extracted for histological analysis and the degree of dentin bridge formation evaluated. Data was analysed by the Kruskal-Wallis test (P<0.05). The pastes and their components were classified in the following decreasing order of antimicrobial action: calcium hydroxide P.A., paste A, paste B and Copaifera langsdorffii Desfon oil. Calcium hydroxide P.A. showed significantly higher antimicrobial action than the pastes or their vehicles. No significant difference was observed between the two pastes in dentin bridge formation. Based on the microorganisms studied, it can be concluded that the pastes analysed showed similar antimicrobial potential but differed significantly from their individual components. No significant difference was observed in dentin bridge formation between the different pastes tested.

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Physicochemical properties and antimicrobial activity of calcium hydroxide pastes in association with other compounds

Brazilian Journal of Oral Sciences ◽

10.20396/bjos.v20i00.8662098 ◽

2021 ◽

Vol 20 ◽

pp. e212098

Author(s):

Mariana Maciel Batista Borges ◽

Mayara Conde Frank ◽

Rafaela Fernandes Zancan ◽

Talita Tartari ◽

Rodrigo Ricci Vivan ◽

...

Keyword(s):

Antimicrobial Activity ◽

Physicochemical Properties ◽

Calcium Hydroxide ◽

Benzalkonium Chloride ◽

Antimicrobial Action ◽

Ph Values ◽

Significant Difference ◽

Confocal Scanning ◽

Confocal Scanning Microscopy

Aim: This study aimed to evaluate the pH, Ca2+ release, solubility, and antimicrobial activity of calcium hydroxide (Ca(OH)2) pastes in association with different substances. Methods: Sixty acrylic teeth (n=10) were filled with pastes that associated Ca(OH)2 with the following substances: benzalkonium chloride 5% (G1) and 50% (G2) both in propylene glycol, arnica glycolic extract (G3), green tea glycolic extract (G4), Calen/PMCC™ (G5), and Calen™ (G6). In the group G1 to G4 were used 1g of Ca(OH)2 powder with 0,8g of vehicle. pH and Ca2+ release was measured after 7, 15, and 30 days. For solubility, micro-CT was used immediately and at the periods of 7, 15, and 30 days. For the antimicrobial analysis, a biofilm of E. faecalis was induced in vitro on bovine dentin discs. Live/dead viability dye and confocal scanning microscopy were used. Results: The highest pH values occurred on the first 7 days, and the G6, G1, G3, and G5 presented the highest pH values at this period (P <0.05). Ca2+ release was higher in all groups at 7 days, with the highest values observed in G1, G5, and G6. The volume of all pastes showed no significant difference in the intragroup analysis at 7 and 15 days (P <0.05). G1 and G2 showed the highest antimicrobial action (P <0.05). For the biovolume, there was difference between the G6 and the other groups (P >0.05) with G1 presenting the lowest values. Conclusion: Benzalkonium chloride 5% increases the antimicrobial action of the Ca(OH2), without impairs physicochemical properties.

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Transplantation of coral fragment, Acropora formosa (Scleractinia)

AQUATIC SCIENCE & MANAGEMENT ◽

10.35800/jasm.0.0.2014.7295 ◽

2014 ◽

pp. 1

Author(s):

Hanny Tioho ◽

Maykel A.J Karauwan

Keyword(s):

Fragment Size ◽

Observation Time ◽

Minimum Size ◽

Average Growth Rate ◽

Average Growth ◽

Significant Difference ◽

Acropora Formosa ◽

Survival And Growth ◽

One Way Anova ◽

Better Than

The minimum size of coral transplants, Acropora formosa, was assessed to support their survival and growth. For this, 150 coral fragments of different sizes (5, 10, 15 cm) were transplanted close to the donor colony. Their survivorship and growth were observed for 12 months. At the end of the observation time, 90% of 15 cm-transplanted coral fragments survived, while the others (10cm and 5 cm) did 86% and 82% respectively. The average growth rate of 5 cm-coral fragments was 0.860 cm/month, while 10 and 15 cm-fragments were 0.984 cm/month and 1.108 cm/month respectively. One-way ANOVA showed that there was significant difference (p<0.05) among the three (5, 10, 15 cm) transplant initial sizes in which the longest fragment size tended to survive longer than the smaller one. However, the smaller transplants grew better than the bigger one, 10.318 cm/year (206%) for 5 cm-transplant, 11.803 cm/year (118%) for 10 cm-transplant, and 13.299 cm/year (89%) for 15 cm-transplant, respectively. Ukuran minimal fragmen karang Acropora formosa yang ditransplantasi diduga untuk mendukung ketahanan hidup dan pertumbuhannya. Untuk itu, 150 fragmen karang ditransplantasi ke lokasi yang berdekatan dengan koloni induknya. Ketahanan hidup dan pertumbuhan semua fragmen karang yang ditransplantasi diamati selama 12 bulan. Pada akhir pengamatan, 90% dari fragmen karang berukuran 15 cm yang ditransplantasi dapat bertahan hidup, sedangkan yang lainnya (ukuran 10 cm dan 5 cm) masing-masing sebesar 86% dan 82%. Rata-rata laju pertumbuhan fragmen karang dengan ukuran awal 5 cm adalah 0,860 cm/bulan, sedangkan ukuran fragmen 10 dan 15 cm masing-masing adalah 0,984 cm/bulan and 1,108 cm/bulan. ANOVA satu arah menunjukkan adanya perbedaan yang nyata (p<0.05) antara ketiga ukuran fragmen yang berbeda, di mana ukuran fragmen karang yang lebih panjang cenderung mempunyai ketahanan hidup yang lebih baik. Namun demikian, ukuran transplant yang lebih kecil memiliki pertumbuhan lebih baik dibandingkan dengan ukuran yang lebih besar, yakni10,318 cm/tahun (206%) untuk transplant berukuran 5 cm, 11,803 cm/tahun (118%) untuk 10 cm, dan 13,299 cm/tahun (89%) untuk ukuran 15 cm.

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Analysis of Support for Parents in Raising Children with Dyslexia

Journal of Family Issues ◽

10.1177/0192513x20948925 ◽

2020 ◽

pp. 0192513X2094892

Author(s):

Athira Amira Abd Rauf ◽

Maizatul Akmar Ismail ◽

Vimala Balakrishnan ◽

Loh Sau Cheong ◽

Novia Indriaty Admodisastro ◽

...

Keyword(s):

Marital Status ◽

Self Esteem ◽

Peer To Peer ◽

Government Support ◽

Anova Analysis ◽

Raising Children ◽

The Social ◽

Significant Difference ◽

Different Types ◽

One Way Anova

The parents of children with dyslexia often experience more parenting stress and depressive symptoms compared to other parents. Studies have shown that supporting and encouraging such parents help in reducing their frustrations, fear, anger, and low self-esteem towards their children. The purpose of this study was to identify and examine the different types of support needed by the parents of children with dyslexia and to explore the relationships between the required support with the parents’ marital status. Fifty questionnaires were distributed to the parents of children with dyslexia and analyzed. The findings showed that the type of support needed for parents of children with dyslexia could be grouped into social, peer-to-peer, financial, and government support. Furthermore, the analysis indicated that there were no significant differences between the social (p = 0.4014), peer-to-peer (p = 0.5020), and government (p = 0.7121) support with parents’ marital status. However, based on one-way ANOVA analysis, there was a significant difference found between the parents’ marital status and financial support (p = 0.0241). Accordingly, it is anticipated that the implication of this research could be used as a guide and a reference for supporting and encouraging parents of children with dyslexia and other learning disabilities.

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Evaluation of the antimicrobial effect of calcium hydroxide combined to different vehicles

Revista Odonto Ciência ◽

10.15448/1980-6532.2016.2.21522 ◽

2016 ◽

Vol 31 (2) ◽

pp. 89

Author(s):

Tatiana Dantas Piana ◽

Maria de Fátima Malvar Gesteira ◽

Erica Dos Santos Carvalho ◽

Josilene Borges Torres Lima Matos ◽

Monica Franca ◽

...

Keyword(s):

Calcium Hydroxide ◽

Enterococcus Faecalis ◽

Antimicrobial Effect ◽

Antimicrobial Action ◽

Diffusion Method ◽

Agar Diffusion ◽

Inhibition Zones ◽

Agar Diffusion Method

Objective: To evaluate the potentiating effect of different substances in antimicrobial action of calcium hydroxide.Methods: The agar diffusion method, was used with well technique, to analyze seven substances associated to calcium hydroxide to make some pastes, they are: chlorexidine, saline, anesthetic, malvatricin, propolis, hypochlorite, paramonochlorophenol.Results: The pastes with malvatricin and paramonochlorophenol presented greater inhibition zones against Enterococcus faecalis.Conclusion: There was potentialization in antimicrobial effect of calcium hydroxide when associated to paramonochlorophenol and to malvatricin especially against Enterococcus faecalis. Low increase of the antimicrobial capacity was observed when using chlorexidine as vehicle to calcium hydroxine paste.

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PENGARUH FASE BULAN TERHADAP WAKTU TEBAR PANCING DAN LAJU TANGKAP MADIDIHANG (Thunnus albacares Bonnaterre, 1788) PADA ARMADA RAWAI TUNA

Jurnal Penelitian Perikanan Indonesia ◽

10.15578/jppi.22.4.2016.207-214 ◽

2017 ◽

Vol 22 (4) ◽

pp. 207

Author(s):

Irwan Jatmiko ◽

Bram Setyadji ◽

Arief Wujdi

Keyword(s):

Full Moon ◽

Yellowfin Tuna ◽

Catch Rate ◽

Lunar Phase ◽

Anova Analysis ◽

Tukey Test ◽

Significant Difference ◽

New Moon ◽

One Way Anova ◽

Tuna Longline

Madidihang/yellowfin tuna merupakan salah satu jenis ikan tuna ekonomis penting bagi industri perikanan di Indonesia dengan kontribusi hasil tangkapan yang terbanyak. Penelitian ini bertujuan untuk mengetahui pengaruh fase bulan terhadap waktu mulai tebar pancing dan laju tangkap madidihang pada armada rawai tuna. Pengumpulan data dilakukan oleh pemantau ilmiah pada armada rawai tuna yang sebagian besar berbasis di Pelabuhan Benoa, Bali mulai Agustus 2005 hingga Juni 2014. Daerah penangkapan ikan dari armada rawai tuna yang diambil datanya berada di lokasi (lintang dan bujur) 9°-16° LS hingga 109°-120° BT. Analisis anova satu arah dan tes Tukey dilakukan untuk mengetahui pengaruh fase bulan terhadap waktu mulai tebar pancing dan laju tangkap madidihang. Total sebanyak 60 trip dan 1.467 hari operasi penangkapan armada rawai tuna dilakukan dalam penelitian ini. Analisis statistik anova satu arah menunjukkan bahwa terdapat pengaruh yang nyata pada fase bulan terhadap waktu mulai tebar pancing (p<0,05). Selanjutnya, tes Tukey menunjukkan bahwa waktu mulai tebar pancing pada saat bulan purnama dimulai pada pukul 9:00 pagi hari. Waktu ini lebih lambat sekitar 2 jam dari pada waktu mulai tebar pancing pada ketiga fase bulan lainnya (perbani awal, perbani akhir dan bulan baru) yang dilakukan sekitar pukul 7:00 pagi hari. Analisis statistik anova satu arah juga menunjukkan terdapat perbedaan yang nyata antar fase bulan terhadap laju tangkap madidihang (p<0,05). Selanjutnya, tes Tukey menunjukkan bahwa laju tangkap pada saat bulan baru dan perbani awal sebesar 0,13 ekor/100 mata pancing atau lebih besar dibandingkan nilai laju tangkap pada saat purnama dan perbani akhir yang hanya sebesar 0,09 ekor/100 mata pancing. Yellowfin tuna is one of the most economically important species for fisheries industry in Indonesia. The objectives of this study are to investigate the effect of lunar phase to the set time start and catch rate of yellowfin tuna on tuna longline vessels. Data collected by scientific observer on tuna longline vessels mainly based in Benoa Port, Bali from August 2005 to June 2014. Fishing ground of sampled longline tuna located from 9°-16° S to 109°-120° E. One-way anova analysis and Tukey test conducted to examine the effect of lunar phase to the set time start and catch rate of yellowfin tuna. A total of 60 trips and 1,467 fishing days of longline tuna fishing vessels operation have been sampled for in this study. One-way anova analysis showed that there was a significant difference of lunar phase to the set time start (p<0.05). Furthermore, Tukey test showed that the starting time for setting during the full moon begins at 9:00 am. Its time was around 2 hours slower than the start of setting of the other three moon phase (first quarter, last quarter and new moon) which start around 7:00 am. One-way anova analysis also showed that there was a significant difference of lunar phase to catch rate of yellowfin tuna (p<0.05). Furthermore, Tukey test confirmed that hook rate on new moon and first quarter was 0.13/100 hooks or 0.4 bigger than hook rate on full moon and last quarter with only 0.09/100 hooks.

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Frequency of Selenomonas Noxia in Oral Microbiota of Obese and Normal Weight People in Duhok-Iraq

Science Journal of University of Zakho ◽

10.25271/sjuoz.2019.7.4.674 ◽

2019 ◽

Vol 7 (4) ◽

pp. 120-124

Author(s):

Roshna M. Qadir ◽

Mahde S. Abdulrahman

Keyword(s):

Oral Bacteria ◽

Normal Weight ◽

Oral Microbiome ◽

Oral Microbiota ◽

Obese People ◽

Overweight People ◽

Significant Difference ◽

Overweight Group ◽

Oral Carriage ◽

Selenomonas Noxia

Obesity represents one of the major problematic health issues worldwide. Recent evidences suggest that obesity is related with the alteration of the oral microbiome. The aim of this study was to measure the salivary bacterial Selenomonas noxia in Duhok population. A total of 155 saliva samples were collected from individuals (aged between 19-35 years) of both genders (86 females and 69 males). The individuals were divided into three groups (obese, overweight, and normal weight) based on their body mass index. Bacterial genomic DNA was extracted from saliva samples. Molecular detections of Selenomonas noxia were performed by the polymerase chain reaction. Among the 155 participants, 34.1% were obese, 26.4% overweight and 39.3% normal weight individuals. The prevalence rate of oral S. noxia among all people was 82.6%. The highest rate of S. noxia was in obese people (86.8%), followed by overweight (85.4%) and normal weight people (77%). The prevalence of S. noxia in overweight people was statistically significant in compare with the normal weight people (p<0.0001). Moreover, the oral carriage of S. noxia was highest among the overweight females (94.5%) followed by obese females (88.9%). However, no significant difference was found compared to males. The result revealed that it is possible to assume that the expansion of S. noxia in saliva is due to obesity. Moreover, the composition of salivary microbiome may lead to the risk that the overweight group is at risk of future obesity. However, further investigations are required with larger sample and participants with different socioeconomic status in order to address the exact link between obesity and oral bacteria. This could lead to a new and promising therapeutic way for improving human's health.

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An Examination of Teachers’ Attitudes Towards the National Park in Their Environment: The Isparta, Denizli Example

Journal of Educational Issues ◽

10.5296/jei.v6i2.17894 ◽

2020 ◽

Vol 6 (2) ◽

pp. 398

Author(s):

Aytaç Karakaş ◽

Hasan Genç

Keyword(s):

Social Sciences ◽

National Parks ◽

National Park ◽

Attitude Scale ◽

Alpha Coefficient ◽

Descriptive Research ◽

Significant Difference ◽

Teachers Attitudes ◽

One Way Anova ◽

Education Process

Environmental education in national parks is equivalent to an open and permanent education process in which individuals and communities become aware of their natural environment and gain a critical and sensitive attitude towards it and the use of its resources. The aim of this study was to examine the attitudes of teachers towards national parks who work in schools near different national parks in terms of different variables. As a work of descriptive research, it was conducted with a total of 167 teachers who worked in schools around Kovada Lake National Park, Kızıldağ National Park, and Honaz Mountain National Park. In the study, an attitude scale with a Cronbach’s Alpha coefficient of 0.83 and consisting of 32 items was used for the national parks. The SPSS (Statistical Package for Social Sciences Program) was used to evaluate the data obtained. A T-test and a one-way ANOVA test were used to analyze the data. According to the results obtained, teachers’ attitudes towards the national park did not show a significant difference in terms of their gender, their tenure, the branches they serve, their purpose of visiting the national park, their opinions on the importance attached to the national park, the different schools near the national park, the national park near the schools they worked in, their purpose in visiting the national park previously while they differed significantly according to whether the teachers had visited the national park before.

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Evaluation of an Innovative Digital Assessment Tool in Dental Anatomy

The Journal of Contemporary Dental Practice ◽

10.5005/jp-journals-10024-1691 ◽

2015 ◽

Vol 16 (5) ◽

pp. 366-371 ◽

Cited By ~ 4

Author(s):

Matt T Lam ◽

So Ran Kwon ◽

Fang Qian ◽

Gerald E Denehy

Keyword(s):

Gold Standard ◽

Repeated Measures ◽

Assessment Tool ◽

T Test ◽

Pearson’S Correlation ◽

Digital Assessment ◽

Dental Anatomy ◽

Significant Difference ◽

Pearson's Correlation ◽

One Way Anova

ABSTRACT Aim The E4D Compare software is an innovative tool that provides immediate feedback to students’ projects and competencies. It should provide consistent scores even when different scanners are used which may have inherent subtle differences in calibration. This study aimed to evaluate potential discrepancies in evaluation using the E4D Compare software based on four different NEVO scanners in dental anatomy projects. Additionally, correlation between digital and visual scores was evaluated. Materials and methods Thirty-five projects of maxillary left central incisors were evaluated. Among these, thirty wax-ups were performed by four operators and five consisted of standard dentoform teeth. Five scores were obtained for each project: one from an instructor that visually graded the project and from four different NEVO scanners. A faculty involved in teaching the dental anatomy course blindly scored the 35 projects. One operator scanned all projects to four NEVO scanners (D4D Technologies, Richardson, TX, USA). The images were aligned to the gold standard, and tolerance set at 0.3 mm to generate a score. The score reflected percentage match between the project and the gold standard. One-way ANOVA with repeated measures was used to determine whether there was a significant difference in scores among the four NEVO scanners. Paired-sample t-test was used to detect any difference between visual scores and the average scores of the four NEVO scanners. Pearson's correlation test was used to assess the relationship between visual and average scores of NEVO scanners. Results There was no significant difference in mean scores among four different NEVO scanners [F(3, 102) = 2.27, p = 0.0852 one-way ANOVA with repeated measures]. Moreover, the data provided strong evidence that a significant difference existed between visual and digital scores (p = 0.0217; a pairedsample t-test). Mean visual scores were significantly lower than digital scores (72.4 vs 75.1). Pearson's correlation coefficient of 0.85 indicated a strong correlation between visual and digital scores (p < 0.0001). Conclusion The E4D Compare software provides consistent scores even when different scanners are used and correlates well with visual scores. Clinical significance The use of innovative digital assessment tools in dental education is promising with the E4D Compare software correlating well with visual scores and providing consistent scores even when different scanners are used. How to cite this article Lam MT, Kwon SR, Qian F, Denehy GE. Evaluation of an Innovative Digital Assessment Tool in Dental Anatomy. J Contemp Dent Pract 2015;16(5):366-371.

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Uji Biokompabilitas pada Implan Orthopedi Antara Implan Impor, Implan Lokal dari Material Impor, dan Prototipe Stainless Steel 316L dari Material Lokal

Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya ◽

10.30651/jqm.v3i1.1612 ◽

2019 ◽

Vol 3 (1) ◽

pp. 7

Author(s):

Ferdiansyah Mahyudin ◽

Heppy Chandra Waskita ◽

Dwikora Novembri Utomo ◽

Heri Suroto ◽

Tri Wahyu Martanto

Keyword(s):

Stainless Steel ◽

Acute Toxicity ◽

Mtt Assay ◽

Orthopedic Implants ◽

Fibrous Capsule ◽

Cytotoxicity Test ◽

Stainless Steel 316L ◽

Medical Test ◽

Significant Difference ◽

One Way Anova

ABSTRACT Demands of orthopedic implants are increasing in Indonesia, but its production are limited and still imported. Stainless steel, especially 316L is one of metal that is used commonly. This research evaluates biocompatibility of stainless steel 316L implant prototype, local implant manufactured using imported stainless steel, and imported stainless steel implant. Cytotoxicity test used mesenchymal human cell with MTT assay. Irritation test, acute toxicity/pyrogenicity test, and implantation test used 24 Wistar rats respectively which were assigned to 4 groups. We evaluate Draize score, weight and rectal temperature changes, and fibrous capsule ring thickness. Cytotoxicity test shows viable cell percentage in stainless steel 316L implant prototype, local implant, and imported implant are 83.7%, 87.5%, and 83.7% respectively. Comparison test using One Way ANOVA shows no significant difference between 3 groups (p>0.05). Iritation test results in 3 groups are grade 0 (no edema and no erythema). Acute toxicity/pyrogenicity test shows no statistically significant difference (p>0.05) among all groups. There is also no significant difference (p>0.05) of fibrous capsule ring thickness on 1 and 4 weeks after implantation. Based on ISO for medical test guide, stainless steel 316L implant prototype is safe and has similar biocompatibility test result compared to local and imported implant.Keywords : stainless steel 316L, biocompatibility, orthopaedic implantCorrespondence to : [email protected] ABSTRAKKebutuhan implan orthopedi terus meningkat di Indonesia, namun produksinya terbatas dan sebagian besar masih impor dari luar negeri. Stainless steel, utama 316L adalah salah satu metal yang sering digunakan. Penelitian ini mengevaluasi biokompatibilitas dari prototipe implan stainless steel 316L, implan lokal yang diproduksi menggunakan stainless steel impor, dan implan stainless steel impor. Uji sitotoksisitas menggunakan sel mesenkim human dengan MTT assay. Uji iritasi, uji pirogenositas, dan uji implantasi menggunakan 24 tikus Wistar yang terbagi dalam 4 grup. Kami mengevaluasi skor Draize, perubahan berat dan temperatur rektal, dan ketebalan fibrous capsule ring. Uji sitotoksisitas menunjukkan persentase viabilitas sel pada prototipe stainless steel 316L, implan lokal, dan implan impor berturut-turut adalah 83,7%; 87,5%; dan 83,7%. Uji komparasi dengan One Way Anova tidak menunjukkan perbedaan signifikan diantara 3 grup (p>0,05). Hasil uji iritasi 3 grup menunjukkan grade 0 (tidak ada edema dan eritema). Uji pirogenositas tidak menunjukkan perbedaan signifikan pada semua grup (p>0,05). Tidak ada perbedaan signifikan (p>0,05) pula pada ketebalan fibrous capsule ring di minggu ke 1 dan 4 setelah implantasi. Berdasarkan ISO untuk pedoman uji di bidang medis, prototipe stainless steel 316L aman dan memiliki kesamaan hasil uji biokompatibilitas bila dibandingkan dengan implan lokal dan impor.Kata kunci : stainless steel 316L, uji biokompatibilitas, implan orthopaediKorespondensi : [email protected]

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RELEVANCE OF VO2max CONSUMPTION IN ELITE BULGARIAN TAEKWONDO COMPETITORS

Journal of Applied Sports Sciences ◽

10.37393/jass.2021.02.3 ◽

2021 ◽

Vol 2 (2021) ◽

pp. 28-39

Author(s):

Dimitar Avramov ◽

Keyword(s):

Heart Rate ◽

Aerobic Fitness ◽

Treadmill Test ◽

Male Athletes ◽

Weight Category ◽

National Team ◽

Significant Difference ◽

The One ◽

Male Subjects ◽

One Way Anova

The aim of this study was to determine aerobic fitness through the VO2max treadmill test of elite Bulgarian taekwondo players with international results, and to determine whether the aerobic system had an effect upon the sports result in taekwondo. Fourteen elite taekwondo athletes, members of the Bulgarian national team (8 male and 6 female) were tested using a continuous progressive treadmill test. Physiological characteristics such as maximal oxygen uptake(VO2max), blood lactate and heart rate were measured. The male athletes recorded 58.2±3.4 ml kg–1 min–1 and the female 46.0±2.8 ml kg–1 min–1. The lactate level reached its highest at the 6’ after the VO2max with results for the males of 11.5±3.7 (mmol l-1) and 9.9±4.1 (mmol l-1) for the females respectively. A comparison between our results, regarding VO2max and previously reported was made using the One-way Anova for independent samples. It showed no significant difference between the male subjects (58.2±3.4 versus 60.7±3.3 ml kg(-1) min(-1), p>.05) and significant difference between the female ones (46.0±2.8 versus 49.8±2.8 ml kg(-1) min(-1), p<.05). Investigated also was the number of kicks executed by the winner of -49 kg weight category and her direct opponents during the 2019 Grand Prix Sofia. It was discovered that the winner kicked an average of 86.25 times per match and her kicks during the Grand Prix Sofia accumulated to 390 in total. It is our conclusion that the aerobic fitness does not play a significant role in taekwondo.

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