scholarly journals Antimicrobial activity of essential oils from Lippia alba, Lippia sidoides, Cymbopogon citrates, Plectranthus amboinicus, and Cinnamomum zeylanicum against Mycobacterium tuberculosis

2018 ◽  
Vol 48 (6) ◽  
Author(s):  
Aquiles Paulino Peres Mota ◽  
João Carlos Pinheiro Dantas ◽  
Cristiane Cunha Frota
Keyword(s):  
Antimicrobial Activity ◽  
Mycobacterium Tuberculosis ◽  
Essential Oils ◽  
Antimycobacterial Activity ◽  
Effective Control ◽  
Cinnamomum Zeylanicum ◽  
Promising Alternative ◽  
Lippia Sidoides ◽  
Lippia Alba ◽  
Plectranthus Amboinicus

ABSTRACT: The rise in cases of antibiotic-resistant Mycobacterium tuberculosis has become a major obstacle to the effective control of tuberculosis (TB) worldwide. Essential oils (EO) are complex mixtures that may contain between 20 and 60 components, with two or three major compounds at relatively high concentrations (20-70%) that are responsible for their pharmacological properties. The objective of this study was to assess the antimicrobial activity of the EOs, bushy lippia (Lippia alba), rosemary pepper (Lippia sidoides), lemon grass (Cymbopogon citratus), Mexican mint or Indian borage (Plectranthus amboinicus), and true cinnamon (Cinnamomum zeylanicum), against Mycobacterium tuberculosis H37Rv. Chemical characterization of the EOs was performed by gas chromatography coupled to mass spectrometry. The minimum inhibitory concentration (MIC) was determined by the microdilution-based resazurin microtiter assay. Four EOs were able to inhibit the growth of M. tuberculosis, with MICs of 286.5±130.2μg/mL (C. zeylanicum), 299.5±117.2μg/mL (L. sidoides), 351.6±39.06μg/mL (P. amboinicus), and 1,250μg/mL (C. citratus). Only the EO of L. alba showed no antimycobacterial activity at the tested concentrations, with an MIC greater than 1,250µg/mL. Results of this study suggested that C. zeylanicum, L. sidoides, and P. amboinicus could be important sources of bactericidal compounds against M. tuberculosis and require further investigation. The activity against M. tuberculosis of these three EOs has not been reported previously. The results show the high potential of the tested antimycobacterial EOs, making them a promising alternative for TB treatment. This data also confirms the importance of bioprospecting studies for active substances with antimycobacterial activity, which are still scarce.

scholarly journals Polyketides from an Endophytic Aspergillus fumigatus Isolate Inhibit the Growth of Mycobacterium tuberculosis and MRSA

2015 ◽  
Vol 10 (10) ◽  
pp. 1934578X1501001 ◽  
Author(s):  
Andrew J. Flewelling ◽  
Amanda L Bishop ◽  
John A. Johnson ◽  
Christopher A. Gray
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Mycobacterium Tuberculosis ◽  
Aspergillus Fumigatus ◽  
Crude Extract ◽  
Antimycobacterial Activity ◽  
Methicillin Resistant ◽  
Bioassay Guided Fractionation ◽  
Chaetoglobosin A

The crude extract of Aspergillus fumigatus isolate AF3-093A, an endophyte of the brown algaFucus vesiculosus, showed significant antimicrobial activity in initial bioactivity screens. Bioassay-guided fractionation of the extract led to the isolation of flavipin, chaetoglobosin A and chaetoglobosin B, all of which inhibited the growth of Staphylococcus aureus, methicillin-resistant S. aureus and Mycobacterium tuberculosis H37Ra. The antimycobacterial activity of these compounds has not been previously reported.

scholarly journals Antimicrobial and antibiofilm activity of the essential oil from dried leaves of Eucalyptus staigeriana

2019 ◽  
Vol 86 ◽  
Author(s):  
Marcos Saldanha Correa ◽  
Joseli Schwambach ◽  
Michele Bertoni Mann ◽  
Jeverson Frazzon ◽  
Ana Paula Guedes Frazzon
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Clinical Samples ◽  
Resistant Bacteria ◽  
Antibiofilm Activity ◽  
Gram Positive ◽  
Promising Alternative ◽  
Gram Negative

ABSTRACT: In recent years, compounds with biological properties produced by plants have received attention as an alternative to control microorganisms. Essential oils extracted from green leaves of Eucalyptus sp. have been demonstrated to have antimicrobial activities, but so far there are no reports of antimicrobial activity of essential oils extracted from dried leaves of Eucalyptus staigeriana. So, the objectives of this study were to determine the chemical composition of the essential oils obtained from dried leaves of E. staigeriana (EOdlES) and to evaluate in vitro antimicrobial and antibiofilm activities of EOdlES against gram-positive and gram-negative, resistance and multiresistant Enterococcus faecalis isolated from food and clinical samples. The characterization of EOdlES was performed by gas chromatography-mass spectrometry (GC/MS). For this study, 26 bacterial strains were used, which included 11 reference strains and 15 antibiotic resistant and multiresistant E. faecalis strains. Antimicrobial activities of EOdlES against gram-positive and gram-negative were determined using the disc diffusion method. The minimum inhibitory concentration (MIC) value was evaluated by a microbroth dilution technique. The antibiofilm effects were assessed by microtiter plate method. As a result, 21 compounds were identified, being oxygenated monoterpenes (69.58%) the major chemical family. EOdlES showed only antimicrobial activity against gram-positive strains. E. faecalis resistant and multiresistant strains show the lowest MIC (3.12 to 6.25%), when compared with reference E. faecalis strain. EOdlES has the ability to inhibit the biofilm formation, but little or none ability to inhibit the preformed biofilm. This study demonstrates that EOdlES is a promising alternative to control important foodborne and clinic gram-positive resistant bacteria.

Antimicrobial activity of essential oils from Lippia sidoides, Ocimum gratissimum and Zingiber officinale against Aeromonas spp.

2020 ◽  
pp. 1-10
Author(s):  
Patrícia Castro Monteiro ◽  
Cláudia Majolo ◽  
Francisco Célio Maia Chaves ◽  
Humberto Ribeiro Bizzo ◽  
Fernanda Loureiro Almeida O’Sullivan ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Zingiber Officinale ◽  
Ocimum Gratissimum ◽  
Lippia Sidoides

scholarly journals Stabilization of Hypoxia-Inducible Factor Promotes Antimicrobial Activity of Human Macrophages Against Mycobacterium tuberculosis

2021 ◽  
Vol 12 ◽  
Author(s):  
Sebastian F. Zenk ◽  
Sebastian Hauck ◽  
Daniel Mayer ◽  
Mark Grieshober ◽  
Steffen Stenger
Keyword(s):  
Antimicrobial Activity ◽  
Mycobacterium Tuberculosis ◽  
Oxygen Sensor ◽  
Expression Patterns ◽  
Hypoxia Inducible Factor ◽  
Antimycobacterial Activity ◽  
P38 Map Kinase ◽  
Microbial Pathogens ◽  
Prolyl Hydroxylases ◽  
Human Macrophages

Hypoxia-inducible factor (HIF) is a key oxygen sensor that controls gene expression patterns to adapt cellular metabolism to hypoxia. Pharmacological inhibition of prolyl-hydroxylases stabilizes HIFs and mimics hypoxia, leading to increased expression of more than 300 genes. Whether the genetic program initialized by HIFs affects immune responses against microbial pathogens, is not well studied. Recently we showed that hypoxia enhances antimicrobial activity against Mycobacterium tuberculosis (Mtb) in human macrophages. The objective of this study was to evaluate whether the oxygen sensor HIF is involved in hypoxia-mediated antimycobacterial activity. Treatment of Mtb-infected macrophages with the prolyl-hydroxylase inhibitor Molidustat reduced the release of TNFα and IL-10, two key cytokines involved in the immune response in tuberculosis. Molidustat also interferes with the p38 MAP kinase pathway. HIF-stabilization by Molidustat also induced the upregulation of the Vitamin D receptor and human β defensin 2, which define an antimicrobial effector pathway in human macrophages. Consequently, these immunological effects resulted in reduced proliferation of virulent Mtb in human macrophages. Therefore, HIFs may be attractive new candidates for host-directed therapies against infectious diseases caused by intracellular bacteria, including tuberculosis.

scholarly journals Antimicrobial Activity of Some Essential Oils against Methicillin-Susceptible and Methicillin-Resistant Staphylococcus pseudintermedius-Associated Pyoderma in Dogs

Animals ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1782 ◽  
Author(s):  
Francesca Paola Nocera ◽  
Simone Mancini ◽  
Basma Najar ◽  
Fabrizio Bertelloni ◽  
Luisa Pistelli ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Multidrug Resistant ◽  
Therapeutic Approaches ◽  
Cinnamomum Zeylanicum ◽  
Staphylococcus Pseudintermedius ◽  
Methicillin Resistant ◽  
Resistant Strains ◽  
The One

This study aimed to test in vitro the antimicrobial activity of 11 essential oils (EOs) against four methicillin-resistant Staphylococcus pseudintermedius (MRSP) and four methicillin-susceptible S. pseudintermedius (MSSP) clinical isolates. The obtained findings demonstrated a clear in vitro efficacy of some tested EOs against both MRSP and MSSP strains. Particularly, modal minimum inhibitory concentration (MIC) values ranging from 1:2048 v/v for Melissa officinalis against an MSSP strain to 1:256 v/v for Cymbopogoncitratus against all MRSP strains were observed. The best results, highlighting a modal MIC value of 1:1024 v/v for all tested isolates, was provided by Cinnamomum zeylanicum. Intriguingly, Cinnamomum zeylanicum showed, in many cases, a correspondence between minimum bactericidal concentration (MBC) and MIC values, indicating that the inhibiting dose is also often bactericidal. Moreover, a mild antibacterial and bactericidal activity against both MRSP and MSSP isolates was detected for the other tested EOs. Considering the zoonotic potential of S. pseudintermedius and the increased dissemination of multidrug-resistant strains, the employment of EOs could be useful for the treatment of canine pyoderma. Since antibiotic resistance has become the most urgent issue, from the perspective of the One Health initiative, alternative therapeutic approaches are desirable to limit the use of antibiotics or to improve the efficacy of conventional therapies.

scholarly journals Composition, anti-quorum sensing and antimicrobial activity of essential oils from Lippia alba

2014 ◽  
Vol 45 (3) ◽  
pp. 759-767 ◽  
Author(s):  
Jesus Olivero-Verbel ◽  
Ana Barreto-Maya ◽  
Angela Bertel-Sevilla ◽  
Elena E. Stashenko
Keyword(s):  
Antimicrobial Activity ◽  
Quorum Sensing ◽  
Essential Oils ◽  
Lippia Alba

scholarly journals ANTIBACTERIAL ACTIVITY OF ESSENTIAL OILS AGAINST PATHOGENS OF IMPORTANCE IN CAPRINE AND OVINE MASTITIS

2021 ◽  
Vol 34 (3) ◽  
pp. 702-708
Author(s):  
RODOLFO FURLANI ◽  
MAURA MARINETE DE SOUSA ◽  
GIOVANNA NOGUEIRA DA SILVA AVELINO OLIVEIRA ROCHA ◽  
FLÁVIA CARTAXO RAMALHO VILAR ◽  
RICARDO CARTAXO RAMALHO ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Plant Species ◽  
Bacterial Resistance ◽  
Small Ruminants ◽  
Bacterial Isolates ◽  
Dairy Goats ◽  
Lippia Alba ◽  
Therapeutic Alternatives ◽  
Staphylococcus Spp

ABSTRACT Mastitis is an important infirmity that affects dairy goats and sheep flocks. Antimicrobials are widely used in mastitis therapy; however, the phenomenon of bacterial resistance has sparked interest in therapeutic alternatives, especially the research on essential oils from several plant species. Therefore, this study aimed to evaluate the antimicrobial activity of essential oils against pathogens of importance in caprine and ovine mastitis. The essential oils were obtained by hydrodistillation and the chemical composition was analyzed by gas chromatography coupled to mass spectrometry (GC-MS). The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) tests were performed to evaluate the antimicrobial activity of the essential oils (EO) of four plants. Fifteen bacterial isolates, previously identified as Staphylococcus spp. and Staphylococcus aureus, were used. The following plant species were used against the 15 bacterial isolates: Lippia origanoides “alecrim pimenta,” Lippia alba “erva cidreira,” Cymbopogon citratus “capim-santo,” and Ocimum basilicum “manjericão.” The first three essential oils presented antimicrobial activity against 100% of the isolates (15/15), with MICs of 560 µg mL-1, 1,173 µg mL-1, and 1,280 µg mL-1, and MBCs of 613 µg mL-1, 1,226 µg mL-1, and 1,333 µg mL-1, respectively. Nevertheless, the essential oil of O. basilicum, which was also tested, exerted no activity against any of the isolates. Carvacrol, citral, geraniol, and estragole were some of the major compounds found. These results can aid other studies that aim to use these essential oils as potential products for subsequent use in the treatment of mastitis in small ruminants.

scholarly journals Essential oils and isolated compounds from Lippia alba leaves and flowers: Antimicrobial activity and osteoclast apoptosis

2014 ◽  
Vol 35 (1) ◽  
pp. 211-217 ◽  
Author(s):  
PAULO JOSÉ LIMA JUIZ ◽  
ANGELICA MARIA LUCCHESE ◽  
ROBERTO GAMBARI ◽  
ROBERTA PIVA ◽  
LETIZIA PENOLAZZI ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Lippia Alba ◽  
Osteoclast Apoptosis
Sign in / Sign up

Export Citation Format

Share Document