scholarly journals Management of white mold of soybean using winter cereal straw

2021 ◽  
Vol 51 (2) ◽  
Author(s):  
Leandro André Feller ◽  
Jessica Vanessa Wosniak Corrêa ◽  
Carla Daiane Leite ◽  
Leandro Alvarenga Santos ◽  
Cacilda Márcia Duarte Rios Faria ◽  
...  
Keyword(s):  
Dry Weight ◽  
White Mold ◽  
Control Treatment ◽  
Production Plant ◽  
Winter Cereal ◽  
Carpogenic Germination ◽  
Cereal Straw ◽  
Winter Cereals ◽  
Rye Straw

ABSTRACT: The management of white mold (Sclerotinia sclerotiorum(Lib.) De Bary) has been one of the main production limitations faced by soybean (Glycine max L.) producers. Considering the complex management of this disease and resistance structure of the pathogen, the present study was conducted in the municipalities of Guarapuava and Palmas in Paraná with the objective of managing the white mold of soybean using straws of winter cereals, such as oat, rye, and triticale. Initially, the three winter cereals were cultivated simultaneously in both the study areas. Straw production, plant height, and shoot fresh and dry weight were evaluated. Subsequently, BMX Apollo soybean was cultivated on cereal straws, and the incidence and severity of white mold were evaluated. In the in vitro experiment, 20 sclerotia covered by a layer of cereal (oat, rye, and triticale) straws were added and carpogenic germination was observed only in the control treatment after 98 days. Regardless of the study site, rye presented greater height and fresh weight than the remaining two cereals. Soybean cultivation on winter cereal straw reduced the incidence and severity of white mold. Cultivation on rye straw reduced mold incidence by 77.7% and 76.6% in Palmas and Guarapuava, respectively.

scholarly journals Production and nutritional value of the wheat silage managed with different cutting systems

2017 ◽  
Vol 38 (1) ◽  
pp. 335 ◽  
Author(s):  
Rodolfo Carletto ◽  
Mikael Neumann ◽  
Danúbia Nogueira Figueira ◽  
Guilherme Fernando Mattos Leão ◽  
Egon Henrique Horst ◽  
...  
Keyword(s):  
Mass Production ◽  
Block Design ◽  
Neutral Detergent Fiber ◽  
Control Treatment ◽  
Lower Amount ◽  
Mineral Matter ◽  
Winter Cereal ◽  
Winter Cereals ◽  
Cereal Species ◽  
Significant Difference

Winter cereals are mainly used for human and animal nutrition and several studies are now exploring their potential as conserved forage (hay and silage). Among the winter cereal species available for cultivation in the south of Brazil, which is major winter cereals producer in the country, the wheat cv. BRS Umbu has attracted special attention. However, few studies have investigated the potential of this cultivar for silage production. The aim of this study was to evaluate the production and quality of silage from the dual-purpose wheat, BRS Umbu, subjected to different cut managements treatments: T1 - without cut (control), T2 - one cut and T3 - two cuts. Each plot represented an experimental unit in a randomized block design, with 5 replicates per treatment. Two representative samples were collected from each plot to determine morphological segmentation (stem, leaf and ear) and dry matter (DM) content of the whole plant and its morphological components. At the time of opening of the silos, food chemical analysis and pH determination were performed. Forage mass production decreased by 26.88% and 67.82%, respectively, with one and two cuts, compared to control. The DM content of the ensiled plant was 49.9 g kg-1 for the control, 54.7 g kg-1 with one cut and 63.2 g kg-1 with two cuts, at the time of ensiling. Management cuts changed the morphological components of the plants, with a lower proportion of stem (28%) in plants subjected to two cuts. The control treatment showed fewer leaves in ensiled plant (9.6%) and intermediate amount of stalk (52.8%), and was significantly different (p < 0.05) to treatment of a cut. Regarding feed chemistry evaluations, no significant difference (p > 0.05) was observed for mineral matter (MM), crude protein (CP) and hemicellulose (HEM) between the different cutting regimens. However, neutral detergent fiber (NDF) and acid detergent fiber (ADF) decreased (p < 0.05) as the number of cuts increased. The control treatment showed higher NDF and ADF content (563.2 and 357.9 g kg-1 DM, respectively) and lower amount of total digestible nutrients (TDN) and net energy of lactation (NEL). However, the estimated milk production (EMP) was superior for this treatment (22,447 l ha-1), demonstrating the high impact of reduced forage mass production with increased number of cuts.

scholarly journals Antagonistic effectiveness of some bacteria against Fusarium graminearum causing crown rot disease on wheat (Triticum aestivum)

2021 ◽  
Vol 13 (1) ◽  
pp. 69-80
Author(s):  
Majida Hadi Mahdi Alsaady ◽  
Hussein Ali Salim ◽  
Rakib A. Al-ani ◽  
Hadi M. Aboud ◽  
Jamal Talib M Al Roubaie
Keyword(s):  
Fusarium Graminearum ◽  
Culture Method ◽  
Dry Weight ◽  
Antagonistic Effect ◽  
Crown Rot ◽  
Control Treatment ◽  
Enzyme Analysis ◽  
Dual Culture ◽  
Rot Disease

In this study, the antagonistic effect of five bacteria genera namely Bacillus, Pseudomonas, Azotobacter, Azospirillum, and Streptomyces isolated from rhizosphere of wheat were evaluated against Fusarium graminearum as potential biocontrol agents in vitro. F. graminearum was molecularly diagnosed using the Polymerase chain reaction (PCR) technique. Each bacteria were tested for the production of catalase enzyme, oxidase enzyme, analysis of starch, analyze of gelatin, and the motility, where Azotobacter, Azospirillum, and Bacillus subtilis were positive for all tested. Fungal inhibition tests were performed by using the dual culture method and agar well diffusion technique. Among them, Streptomyces and Azospirillum exhibited potent inhibition to the growth of F. graminearum (72.14% and 66.42%) respectively, followed by B.pumillus, P.fluorescens, B. subtilis and Azotobacter ( 58.28%, 43.23%, 39.71% and 35.71%) respectively as compared with the control treatment (0.0%).The dry weight of the fungus biomass was decreased with bacteria P. fluorescens, Streptomyces sp, Azotobacter sp, Azospirillum sp, B. subtilis, and B. pumillus which reached (0.114, 0.103, 0.147, 0.101, 0.143, and 0.107 g) respectively compared to the control treatment that was 0. 665 g.

scholarly journals Growth and Photosynthetic Capability of Momordica grosvenori Plantlets Grown Photoautotrophically in Response to Light Intensity

HortScience ◽  
2009 ◽  
Vol 44 (3) ◽  
pp. 757-763 ◽  
Author(s):  
Meijun Zhang ◽  
Duanduan Zhao ◽  
Zengqiang Ma ◽  
Xuedong Li ◽  
Yulan Xiao
Keyword(s):  
Callus Formation ◽  
Dry Weight ◽  
Control Treatment ◽  
Photon Flux ◽  
Photon Flux Density ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Light Intensities ◽  
Four Levels

Momordica grosvenori plantlets were cultured in vitro for 26 d on sucrose- and hormone-free Murashige and Skoog (MS) medium with four levels of photosynthetic photon flux density (PPFD), namely 25, 50, 100, or 200 μmol·m−2·s−1, and a CO2 concentration of 1000 μmol·mol−1 in the culture room [i.e., photoautotrophic micropropagation (PA) treatments]. The control treatment was a photomixotrophic culture using MS medium containing sucrose and NAA with a CO2 concentration of 400 μmol·mol−1 in the culture room and a PPFD of 25 μmol·m−2·s−1. Based on the results, a second experiment was conducted to investigate the effects of α-naphthaleneacetic acid (NAA) and sucrose on callus formation. For this, plantlets were grown in the absence and presence of either NAA or sucrose. Compared with the control, the PA plantlet had a well-developed rooting system, better shoot, greater chlorophyll content, and higher electron transport rate and the ex vitro survival percentage was increased by 31%. Both sucrose and NAA stimulated callus formation on the shoot bases of control plantlets, whereas calluses did not form on the plantlets grown in sucrose- and hormone-free medium. The stronger light intensities increased the fresh and dry weight of plantlets. A PPFD of 100 μmol·m−2·s−1 was more suitable for the growth of M. grosvenori plantlets. Therefore, photoautotrophic plantlets grown at high light intensities would be better suited to the intense irradiance found in sunlight.

scholarly journals Evaluation of different growth hormones mediated callus induction and regeneration as well as the effect of colchicines, ethyl methanesulfonate (EMS) and gamma radiation on some traits of Impatiens walleriana

Genetika ◽  
2021 ◽  
Vol 53 (1) ◽  
pp. 379-391
Author(s):  
Sedigheh Sharafi ◽  
Pejman Azadi ◽  
Maryam Jafarkhani Kermani ◽  
Vahid Abdossi ◽  
Ali Eskandri
Keyword(s):  
Dry Weight ◽  
Control Treatment ◽  
Lower Survival Rate ◽  
Randomized Design ◽  
Effective System ◽  
Number Of Leaves ◽  
Impatiens Walleriana ◽  
The Impact ◽  
Disease Free

Impatiens is an ornamental member of family Balsaminaceae. This plant mostly propagated by vegetative technique, which generally time wasting process. It is often multiplicities via seed but is barricaded by F1 seed sterility. In vitro culture of Impatiens walleriana has much significant function in fast proliferation with useful features and elicitation of healthful and disease-free plants. This experiment was conducted to investigate the impact of medium and different hormones on in vitro propagation of Impatiens walleriana by using a completely randomized design. MS medium was prepared along with various concentrations of BAP, TDZ and ZEA. Callus was induced and grew well in media supplemented with 0.5 mg/l NAA + 1 mg/l BAP. In order to indirect propagation, explants were cultured in same media containing BAP, ZEA and TDZ in combination with NAA. These treatments have ability to organogenesis. The results revealed that the control treatment had the lowest effect on traits including shoot percentage, number of shoots, number of leaves, shoot length, fresh and dry weight, and it lead to maximum proliferations in medium supplemented with 0.5 mg/l NAA + 1 mg/l BAP. The highest root length and rooting percentage was observed in 0.5 mg/l IBA + 0.5 mg/l BAP. In addition, the effect of mutation agents was studied. Aseptic samples were treated with ?- irradiation, Ethyl Methane Sulfonate and colchicines at growth chamber. Treatments with 30 and 60 grey respectively had the lower survival rate, growth rate and polyploidy while colchicines with 0.1 and 0.2 had the highest rats. Regarding to these, the present technique illustrate an effective system for in vitro reproduction of Impatiens walleriana by hypocotyls cultures. In addition, colchicines proved to be effective in induction of polyploidy in this plantlet.

scholarly journals Antifungal activity of homeopathic medicines against the white mold causing agent Sclerotinia sclerotiorum

2021 ◽  
Vol 43 ◽  
pp. e56548
Author(s):  
Ana Carolina Botelho dos Reis ◽  
Júlia Ronzella Ottoni
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
Inhibitory Effect ◽  
White Mold ◽  
Control Treatment ◽  
Antifungal Effect ◽  
Randomized Design ◽  
Significant Yield ◽  
Alternative Techniques ◽  
Completely Randomized Design

White mold disease, caused by fungus Sclerotinia sclerotiorum (Lib.) de Bary., is a disease hard to control due to the high amount of sclerotia produced, which guarantees its survival in the soil for years leading to significant yield losses. Alternative techniques to control the pathogen have been researched, including homeopathy. The present work aimed to evaluate the in vitro antifungal effect of homeopathic medicines on S. sclerotiorum mycelial growth. Homeopathic medicines Sulphur, fungal sclerotium Nosode and Calcarea carbonica, in 30CH, 200CH and 1000CH dynamizations were tested. Assays were carried out in a completely randomized design, with four repetitions. Experiments were performed through the addition of homeopathic medicines on the surface of plates containing culture medium, followed by insertion of a disc containing fungus mycelia and incubation. Control treatment received no homeopathic medicine. The mycelial progression was monitored by seven halo diameter measurements during experiment period. All homeopathic medicines tested and their dynamizations were able to inhibit partially the development of the fungus. Calcarea carbonica at the dynamization of 1000 CH showed the best inhibitory effect on S. sclerotiorum, which under its effect produced a mycelial halo 40% smaller than the control treatment.

scholarly journals Influence of sucrose and tyrosine on callus induction and morphine and codeine production on Papaver somniferum In vitro

2010 ◽  
Vol 4 (2) ◽  
pp. 45-53
Author(s):  
M . S . Hamad ◽  
I. A. Hamza ◽  
S . A . Al-Muktar
Keyword(s):  
Tissue Culture ◽  
Callus Induction ◽  
Drug Research ◽  
Dry Weight ◽  
Papaver Somniferum ◽  
Research Center ◽  
Control Treatment ◽  
Ms Medium ◽  
College Of Agriculture

A study was conducted at the tissue culture lab. College of Agriculture/ University of Baghdad and Drug Research Center/ Health Minstry during 2006 - 2007. Results indicated that MS medium supplemented with 30 g/l of sucrose gave the highest values (630.3, 61.6) mg of both fresh and dry weight of callus respectively. However, values of both parameters were reduced with increasing sucrose concentrations up to 60, 90, 120 g/l (478.2, 346.5, 217.6) mg respectively. MS medium supplemented with 30 mg/l of tyrosine gave the highest values of callus fresh and dry weight (429.7, 42.6) mg respectively. MS medium supplemented with 30 g/l of sucrose and 30 mg/L tyrosine gave the highest values (688.0, 67.5) mg of both fresh and dry weight callus. MS medium supplemented with 30 mg/l of tyrosine and 90 g/l of sucrose gave the highest values (2.9, 2.8) mg/g of morphine and codeine from fresh callus weight respectively while the lowest values of morphine and codeine (0.5K 0.2) mg/g from fresh callus weight respectively of control treatment .

scholarly journals The effect of gamma radiation on callus growth and plantlet regeneration of four tomato hybrid Lycopersicon esculentum mill In vitro

2009 ◽  
Vol 3 (1) ◽  
pp. 56-62
Author(s):  
AssRana A.H. Al-roomia
Keyword(s):  
Lycopersicon Esculentum ◽  
Gamma Radiation ◽  
Dry Weight ◽  
Plantlet Regeneration ◽  
Callus Growth ◽  
Control Treatment ◽  
Ms Medium ◽  
Lycopersicon Esculentum Mill

Calluses were induced from four tomato hybrid (Davista, superDavista, Muntcarloo,Hymar). On MS medium supplemented with 2, 4-D Auxin at concentration of 4 mg/L.The Callus were divided into two groups the first one was affected with gammaradiation at the doses (0,2,4,6,8)Gray and re cultured on the same mentioned medium.The second group was also affected with gamma radiation with the same dosesmentioned above and re- cultured on MS media supplemented with 0.5 mg/L Kinetinfor regeneration. Fresh and dry weight was measured after 8 weeks. The number andlength of shoots were measured as indicators after 6 weeks. Results revealed that thedose 2 Gray was superior to give highest rate of fresh and dry weight reaching 238.5and 43.1 mg respectively and the hybrid super Davista was superior in giving thehighest fresh and dry weight reaching 231.6 and 30.2 mg respectively. Moreover theresults revealed that control treatment was superior in giving the highest rate innumber and length of plantlets. The 2 Gray treatment showed a significant effect onthe rate and length of plantlets. In addition, there were significant differences amongthe cultivars in most characters mentioned above.

scholarly journals Antimycotal Activity of Collimonas Isolates and Synergy-Based Biological Control of Fusarium Wilt of Tomato

2020 ◽  
Vol 4 (1) ◽  
pp. 64-74 ◽  
Author(s):  
Hung K. Doan ◽  
Nilesh N. Maharaj ◽  
Kaitlyn N. Kelly ◽  
Eugene M. Miyao ◽  
R. Michael Davis ◽  
...  
Keyword(s):  
Fusarium Wilt ◽  
Field Experiments ◽  
Pathogenic Fungi ◽  
Dry Weight ◽  
Control Treatment ◽  
Protective Properties ◽  
Shoot Dry Weight ◽  
Geographic Origins ◽  
Wide Range

In a continued effort to discover microorganisms with plant-protective properties, we characterized a collection of bacterial isolates from multiple geographic origins and belonging to the genus Collimonas, for their ability to inhibit in vitro the growth of a wide range of plant-pathogenic fungi and oomycetes. Isolate Collimonas arenae Cal35 showed the greatest all-round performance and was the single-best inhibitor of several of the tested pathogens, including Fusarium oxysporum f. sp. lycopersici, the causal agent of Fusarium wilt of tomato. In greenhouse trials, neither C. arenae Cal35 nor the control treatment, i.e., the commercially available Bacillus-based biofungicide Serenade Soil, was able to prevent symptom formation on tomato plants challenged with F. oxysporum f. sp. lycopersici. However, a mixture of Cal35 and Serenade Soil (referred to as Collinade) significantly reduced vascular discoloration and F. oxysporum f. sp. lycopersici-induced loss of shoot dry weight. In replicated field experiments, a Collinade application reduced vascular symptoms as well as the relative abundance of F. oxysporum in the root endosphere of F. oxysporum f. sp. lycopersici-challenged plants. We discuss the emerging property of “biocombicontrol” by Collimonas and Bacillus in the context of synergy-based formulations for the protection of crops against pathogens.

scholarly journals Carbon Dioxide Enrichment during Photoautotrophic Micropropagation of Protea cynaroides L. Plantlets Improves In Vitro Growth, Net Photosynthetic Rate, and Acclimatization

HortScience ◽  
2013 ◽  
Vol 48 (10) ◽  
pp. 1293-1297 ◽  
Author(s):  
How-Chiun Wu ◽  
Chun-Chih Lin
Keyword(s):  
Dry Weight ◽  
Control Treatment ◽  
Dry Matter Accumulation ◽  
Ms Medium ◽  
In Vitro Growth ◽  
Shoot Dry Weight ◽  
Survival Percentage ◽  
Photoautotrophic Micropropagation ◽  
Photosynthetic Ability

The effects of CO2 enrichment on the in vitro growth and acclimatization of Protea cynaroides L. plantlets were investigated. Three CO2 enrichment concentrations were used: 0 (control), 1000, 5000, and 10000 μmol·mol−1. Plantlets in the control treatment were cultured on half-strength Murashige and Skoog (MS) medium supplemented with sucrose, whereas those enriched with different CO2 concentrations were grown on sucrose-free MS medium. Compared with the control, significant improvements were observed in the growth of plantlets enriched with CO2 irrespective of the concentration. Plantlets enriched with 5000 μmol·mol−1 CO2 produced the highest number of leaves and the largest leaf area. In addition, the photosynthetic ability of plantlets enriched with CO2 was enhanced, which resulted in significant increases in shoot growth and dry matter accumulation. In particular, the shoot dry weight of plantlets cultured in 5000 μmol·mol−1 CO2 and 10000 μmol·mol−1 CO2 were, respectively, 2.1 and 4.2 times higher than those without CO2 enrichment. During acclimatization, the survival percentage, rooting percentage, and leaf number of plantlets grown in elevated CO2 were, respectively, up to 4.5, 1.8, and 2.7 times higher than plantlets without CO2 enrichment. The improvements in survival percentage and ex vitro growth of these plantlets were the result of their enhanced photosynthetic ability in vitro, which resulted in the production of high-quality plantlets. Significant improvements in the overall growth of P. cynaroides plantlets were achieved through the use of photoautotrophic micropropagation with CO2 enrichment.

Effect of the endophytic plant growth promoting Enterobacter ludwigii EB4B on tomato growth

2020 ◽  
Vol 13 (2) ◽  
pp. 54-65 ◽  
Author(s):  
M.E.A. Bendaha ◽  
H.A. Belaouni
Keyword(s):  
Root Length ◽  
Biocontrol Agent ◽  
Growth Promotion ◽  
Dry Weight ◽  
Antagonistic Activity ◽  
Rrna Gene ◽  
Plant Growth Promoting ◽  
Enterobacter Ludwigii ◽  
Tomato Growth

SummaryThis study aims to develop a biocontrol agent against Fusarium oxysporum f.sp. radicis-lycopersici (FORL) in tomato. For this, a set of 23 bacterial endophytic isolates has been screened for their ability to inhibit in vitro the growth of FORL using the dual plate assay. Three isolates with the most sound antagonistic activity to FORL have been qualitatively screened for siderophore production, phosphates solubilization and indolic acetic acid (IAA) synthesis as growth promotion traits. Antagonistic values of the three candidates against FORL were respectively: 51.51 % (EB4B), 51.18 % (EB22K) and 41.40 % (EB2A). Based on 16S rRNA gene sequence analysis, the isolates EB4B and EB22K were closely related to Enterobacter ludwigii EN-119, while the strain EB2A has been assigned to Leclercia adecarboxylata NBRC 102595. The promotion of tomato growth has been assessed in vitro using the strains EB2A, EB4B and EB22K in presence of the phytopathogen FORL. The treatments with the selected isolates increased significantly the root length and dry weight. Best results were observed in isolate EB4B in terms of growth promotion in the absence of FORL, improving 326.60 % of the root length and 142.70 % of plant dry weight if compared with untreated controls. In the presence of FORL, the strain EB4B improved both root length (180.81 %) and plant dry weight (202.15 %). These results encourage further characterization of the observed beneficial effect of Enterobacter sp. EB4B for a possible use as biofertilizer and biocontrol agent against FORL.

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