Keeping Quality and Flavor, and Microorganisms, Proteases and Lipases in Raw Cow and Goat Milk at Collection and After Storage

1983 ◽  
Vol 46 (10) ◽  
pp. 873-877 ◽  
Author(s):  
LESTER HANKIN ◽  
DONALD SHIELDS
Keyword(s):  
Protein Degradation ◽  
Goat Milk ◽  
Raw Milk ◽  
Cow Milk ◽  
Coliform Bacteria ◽  
Microbial Count ◽  
Retail Sale ◽  
Milk Samples ◽  
Significant Difference ◽  
Keeping Quality

Raw cow and goat milks for retail sale in Connecticut were examined at collection and after storage at 4.4 and 7.2°C for 7 d for keeping quality, flavor, microorganisms, protein degradation on storage, and protease and lipase activity. Some milks were bottled at the farm, others were placed in containers supplied by the customer. Goat milk retained a satisfactory flavor significantly longer than cow milk. There was no correlation of keeping quality with any microbial count made at collection except for number of coliform bacteria. Significant differences were found in enzyme activity and protein degradation between cow and goat milk. No significant difference was found between milk bottled at the farm and that collected in sterile containers. Over 82% of raw milk samples met the 30,000 per ml state standard for total aerobic count (SPC) and 72% met the coliform standard of 50 per ml.

COMPARATIVE EVALUATION OF YOGHURT SAMPLES FROM GOATS AND COWS MILK AND COMMERCIAL RETAIL OUTLET

2021 ◽  
Vol 34 (1) ◽  
Author(s):  
V. A. Obatolu ◽  
E. A. Adebowale ◽  
F. Omidokun ◽  
E. O. Farinde
Keyword(s):  
Goat Milk ◽  
Storage Period ◽  
Total Solid ◽  
Cow Milk ◽  
Microbial Count ◽  
Significant Drop ◽  
Overall Acceptability ◽  
Count Range ◽  
Significant Difference ◽  
Mineral Acidity

This paper compared the chemical (Proximate and mineral), acidity, microbial count and organoleptic properties of yoghurt samples produced from Nigeria locally bred goat and cow milk with commercially retail yoghurt during 14days of refrigerated storage. Yoghurt production was conducted by inoculation of the milk samples at 43oC for 6hrs with 2% of streptococcus thermophillus and lactobacillus bulgarus active culture. The total ash content of yoghurt ranges from 0.23g/100g in the commercial yoghurt sample to 0.84g/100g in yoghurt from goat  milk which is considered significantly higher than that observed for yoghurt from cow milk. The commercially retail yoghurt had a significantly higher (P < 0.05) total solid matter than yoghurt from goat and cow milk. Calcium and phosphorus contents were significantly (P < 0.05) lowest in the commercial yoghurt (0.28%) to a significant higher value of 0.28% in goat milk. A statisitically significant drop in pH values was observed in the commercial yoghurt sample after one and two week storage compared to yoghurt from goat and cow milk. High lactic acid bacteria were observed in yoghurt from cow milk with lactococci group being higher than the lactobacilli group throughout the storage period. The yeast count range from a significant low (P < 05) value of 2.22 cfu/ml-1 in commercial yoghurt sample to 3.22 and 3.24 in goat and cow milk respectively on day zero of storage. By the 7days of storage, the commercially retail yoghurt was significant (P < 0.05) least desirable with respect to colour, taste and overall acceptability while there was no significant difference in the consistency of all the yoghurt samples.

scholarly journals Proteolytic and lipolytic potential of Pseudomonas spp. from goat and bovine raw milk

2018 ◽  
Vol 38 (8) ◽  
pp. 1577-1583 ◽  
Author(s):  
José C. Ribeiro Júnior ◽  
Pedro I. Teider Junior ◽  
André L.M. Oliveira ◽  
Edson A. Rios ◽  
Ronaldo Tamanini ◽  
...  
Keyword(s):  
Bovine Milk ◽  
Goat Milk ◽  
Raw Milk ◽  
Species Level ◽  
Cow Milk ◽  
Production Potential ◽  
Gram Negative ◽  
Pseudomonas Spp ◽  
Milk Samples ◽  
The Mean

ABSTRACT: Pseudomonas, the main genus of gram-negative microorganisms isolated from milk, is psychrotrophic, biofilm-forming, and thermo-resistant deteriorating enzyme producers. The aim of this study was to quantify Pseudomonas spp. in goat’s and cow’s milk produced in the Paraná state, Brazil, to evaluate the deteriorating activity of the isolates at mesophilic and psychrotrophic conditions and to identify, at the species level, the isolates with alkaline metalloprotease (aprX gene) production potential. Microbiological, biochemical and molecular methods were used for isolating, confirming and identifying of isolates. The mean counts were 1.6 (±6.3)x104 and 0.89(±3)x102 CFU/mL for goat and bovine milk samples, respectively, immediately after milking. Of the Pseudomonas colonies isolated from goat milk (n=60), 91.7% showed proteolytic potential when incubated at 35°C/48 h and 80% at 7°C/10 days, and lipolytic potential was observed in 95% of the isolates incubated in mesophilic and 78.3% at refrigeration conditions. From the isolates of bovine milk (n=20), 35% showed proteolytic activity only when incubated at 35°C/48 h, and lipolytic potential was observed in 25% of the isolates incubated at 7°C/10d and 35°C/48h. It was observed that 83.3% and 25% of the isolates genetically confirmed as Pseudomonas spp. of goat and bovine milk showed the potential for alkaline metalloprotease production, with the species P. azotoformans, P. koreensis, P. gessardii, P. monteilii and P. lurida being the most frequent in goat milk and P. aeruginosa the only species identified in cow milk.

scholarly journals Comparative study of some indicator bacteria in cows and buffaloes raw milk in Al-Diwaniyah Province

2012 ◽  
Vol 11 (3) ◽  
pp. 46
Author(s):  
K. H. Abass
Keyword(s):  
Winter Season ◽  
Bacterial Count ◽  
Raw Milk ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Winter Period ◽  
City Center ◽  
Summer Period ◽  
Milk Samples ◽  
Significant Difference

The present study was carried out in AL-Diwaniyah city and it's different districts whichincludes (city center, Afak district and their regions, AL-hamza district and their regions, ALShamiadistrict and their regions and AL-Daghara regions and its villages) during a periodNovember 2010 to July 2010. A total of samples used in this study were 120 raw milksamples collected includes 60 samples for each species for (cows and buffaloes) for twodifferent season of the year (Winter and Summer), first start from (beginning November 2009 toend of the January 2010) ,and the second start from (beginning May to July 2010) The resultsof this study revealed the rising the averages of microbes contaminated raw milk samples ineach species of animals included in this study,where raw milk samples for cows recorded210 4.2х CFU/ml , buffaloes 214 12х CFU/ml,The highest averages of total Coliform bacterialcount were found in raw milk samples of cows 214 4.3х CFU/ml and less averages in rawsamples of buffaloes 214 6.1х CFU/ml . While averages of Staphylococcus aureas were highin raw milk samples of buffaloes 0.49 х216CFU/ml and less in raw milk samples of cows0. 214 61х CFU/ ml.While the averages of Streptococcus spp. were 0.37х 216CFU/ml, 0.38 21х6 CFU/ml, for cows and buffaloes respectively. Seasons of the year had an effect on average oftotal bacterial count studied for each sample of raw milk for all species of animals includedin this study.Where shown rising averages of microbes numbers during the summer period(May, Jun, and July), while there are decreasing in an average of microbes during the winter period(November, December, and January).Summer season recorded significant difference ascompared with winter season but averages of total coliform bacteria had no significantdifference between the two seasons.

scholarly journals Aflatoxin M1 Contamination in Raw Cow Milk of Dairy Farms in Behbahan area, Khuzestan province, Iran

2019 ◽  
Author(s):  
Seyedeh Ommolbanin Ghasemian
Keyword(s):  
Dairy Farms ◽  
Raw Milk ◽  
Cow Milk ◽  
Aflatoxin M1 ◽  
Natural Occurrence ◽  
Environmental Chemical ◽  
Khuzestan Province ◽  
Milk Samples ◽  
Significant Difference ◽  
Southwest Of Iran

Introduction: Mycotoxins are among the environmental chemical agents with toxic effects on animals and humans. Aflatoxins (AF) are a major group of mycotoxins produced following the growth of some species of Aspergillus flavus and Aspergillus parasiticus, particularly in agricultural products. Aflatoxin M1 (AFM1) is secreted to milk and remains there since the process of pasteurization and sterilization has little effect on this metabolite. The aim of this study was to investigate the natural occurrence of AFM1 in raw milk in Behbahan area, which is an area of high-density livestock production in southwest of Iran. The other aim was to compare these obtained levels with the accepted level recommended by the European community (50 ng/L). Methods: In the present study, 90 samples of raw milk were collected from five dairy farms in Behbahan region, Khuzestan province, Iran. The AFM1 was analyzed using the Enzyme Linked Immune Sorbent Assay (ELISA) method by ELISA kits from Euro Clone, Italy.  Data were analyzed by ANOVA and the results were reported as mean ± SE. The significant level was set at p ≤ 0.05.  Results: The concentration of aflatoxin M1 in (15)16.54% of raw milk was higher than maximum tolerance limit accepted by European Union /Codex Alimentarius Commission (50 ng/l). The results of this study showed that all 90 investigated raw milk samples were contaminated with AFM1 (100%). Conclusion: A significant difference was observed between concentration means of AFM1 in various studied farms at P < 0.05. Consequently, high occurrence of AFM1 in raw milk samples was considered as a possible hazard for human health. However, further longitudinal studies are required on milk samples and other dairy products in Behbahan as well as other regions of Iran to estimate the representative intake of AFM1 in Iran.

scholarly journals Bacteriological quality and safety of raw cow milk in Madurai (South India)

2013 ◽  
Vol 48 (2) ◽  
pp. 109-114 ◽  
Author(s):  
S Lingathurai ◽  
P Vellathurai
Keyword(s):  
Microbiological Quality ◽  
Raw Milk ◽  
Cow Milk ◽  
Coliform Bacteria ◽  
Plate Count ◽  
Quality And Safety ◽  
E Coli ◽  
Milk Samples ◽  
Total Plate Count ◽  
The Mean

The microbiological quality and safety of raw milk from 60 dairy farms in Madurai were determined. Milk samples were collected at 60 centers from four regions, namely northern, eastern, western and southern (NEWS) according to stratified random sampling design. Samples were analyzed for Total plate count (TPC), psychrotrophs, thermophiles, Staphylococcus aureus, coliform, Escherichia coli 0157: H7 and Salmonella. The mean counts per ml for TPC, psychrotrophs and thermophiles were 12.5x106, 5x103 and 6.85x103 respectively. From the 60 milk samples tested, coliform bacteria contaminated approximately 90% and 70% were E. coli positive, with mean counts ranged from 103 to 104 cfu ml-1. S. aureus was isolated from more than 61.7% of the samples and the mean count per ml was 6.2x103. Meanwhile, E. coli 0157: H7 was also detected in 39 (65%) samples. However, Salmonella was only detected in 8 (13.3%) of the samples with the southern region having the highest frequency of isolation. Bangladesh J. Sci. Ind. Res. 48(2), 109-114, 2013 DOI: http://dx.doi.org/10.3329/bjsir.v48i2.15741

scholarly journals The effect of season on the microbiological status of raw milk

2020 ◽  
pp. 95-99
Author(s):  
Flóra Mária Szabóné Petróczki ◽  
Béla Béri ◽  
Ferenc Peles
Keyword(s):  
Microbiological Quality ◽  
Raw Milk ◽  
Coliform Bacteria ◽  
Plate Count ◽  
Cow’S Milk ◽  
Cow's Milk ◽  
Milk Samples ◽  
Significant Difference ◽  
The Mean ◽  
Raw Cow’S Milk

Many factors can influence the microbiological quality of raw cow’s milk. In this study, our aim was to determine whether there was any difference between the microbiological statuses of milk produced in different seasons. Samples were collected and analysed from five dairy farms in Hajdú-Bihar County, from February to November in 2019. During our studies, total plate count (TPC), coliform count and Staphylococcus aureus count of raw cow’s milk samples were determined. There was no significant difference (P>0.05) between the mean TPC values detected in the milk collected in winter and autumn, but that values were significantly (P<0.05) lower than in the milk samples collected in spring and summer. Similarly to the TPC, in the case of coliform bacteria the lowest mean colony count was detected in the samples collected in winter. The difference was significant (P<0.05), compared to the values observed in the samples collected in summer. S. aureus was detected in bulk milk of only two farms in excess of 1.0 log10 cfu/ml. Also in case of S. aureus, there was a significant difference (P<0.05) between the values observed in the samples collected in winter and in summer. Samples from spring and summer contained the highest amount of S. aureus. Based on the results of our studies, in the case of almost all farms the mean TPC, coliform and S. aureus counts were lower in the samples collected in winter, than in the samples collected in summer. The fact that the samples collected in winter contained the lowest amount of colonies could be attributed to the inhibition of growth of mesophilic microorganisms below 8 °C. Furthermore, the fact that we observed the highest colony counts in samples collected in summer, can be related to the heat stress of cows during the summer due to unfavorable weather conditions (high temperature and humidity).

scholarly journals Growth and enterotoxin production of Bacillus cereus in cow, goat, and sheep milk

2014 ◽  
Vol 83 (10) ◽  
pp. S3-S8 ◽  
Author(s):  
Lenka Necidová ◽  
Šárka Bursová ◽  
Alena Skočková ◽  
Bohdana Janštová ◽  
Pavla Prachařová ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Storage Temperature ◽  
Goat Milk ◽  
Storage Conditions ◽  
Cow Milk ◽  
Enzyme Linked Immunosorbent Assay ◽  
Plate Method ◽  
Milk Samples ◽  
Sheep Milk ◽  
Enterotoxin Production

The aim of this study was to compare Bacillus cereus growth rates and diarrhoeal enterotoxin production in raw and pasteurized goat, sheep, and cow milk in terms of storage conditions. Milk samples were inoculated with B. cereus (CCM 2010), which produces diarrhoeal enterotoxins. Enterotoxin production was tested by ELISA (Enzyme-Linked Immunosorbent Assay), and the count of B. cereus was determined by the plate method. With raw cow milk, B. cereus growth and enterotoxin production can be completely suppressed; in raw goat and sheep milk, enterotoxin was produced at 22 °C. In pasteurized cow, goat, and sheep milk, the B. cereus count increased under all storage conditions, with more rapid growth being observed at 15 °C (sheep milk) and 22 °C (cow and goat milk). Enterotoxin presence was detected at 15 °C and 22 °C, and with pasteurized cow milk also at 8 °C. Our model experiments have determined that B. cereus multiplication and subsequent enterotoxin production depend on storage temperature and milk type.

Occurrence of aflatoxin M1 in bovine milk and associated risk factors among dairy farms of Punjab, India

2021 ◽  
pp. 1-10
Author(s):  
H. Thukral ◽  
P. Dhaka ◽  
J. Singh Bedi ◽  
R. Singh Aulakh
Keyword(s):  
Risk Factors ◽  
European Union ◽  
Bovine Milk ◽  
Dairy Farms ◽  
Buffalo Milk ◽  
Cow Milk ◽  
Milk Samples ◽  
Significant Difference ◽  
Associated Risk Factors ◽  
Mean Concentration

Aflatoxin M1 (AFM1) contamination in milk and milk products may pose a major public health concern. The present cross-sectional study was aimed to estimate the prevalence of AFM1 in bovine milk across all districts of Punjab, India and to identify the associated animal and farm level risk factors. A total of 402 milk samples (266 cow milk and 136 buffalo milk) were analysed using commercial ELISA and representative samples were confirmed using HPLC-FLD. The results revealed that 56.2 and 13.4% of the milk samples exceeded the maximum levels of the European Union, i.e. 0.05 μg/l and Food Safety and Standards Authority of India (FSSAI), i.e. 0.5 μg/l for AFM1 in milk, respectively. On analysis of species variation, buffalo milk (prevalence: 56.6%; mean concentration: 0.42±0.9 μg/l) was found to have higher AFM1 levels than cow milk (prevalence: 56.0%; mean concentration: 0.19±0.3 μg/l), with statistically significant difference between mean concentrations (P<0.01) and non-significant difference between AFM1 prevalence (P=0.91). Furthermore, milk from commercial dairy farms (prevalence: 64.7%; mean concentration: 0.34±0.65 μg/l) was found to be more contaminated than from household dairy establishments (prevalence: 47.8%; mean concentration: 0.19±0.65 μg/l). The risk factors ‘above average milk yield/day’ (odds ratio (OR): 2.4) and ‘poor animal hygiene’ (OR: 1.9) were identified at animal level, and ‘intensive dairy farming’ (OR: 3.1) and ‘animal feed without aflatoxin binder’ (OR: 4.7) as farm level risk factors for AFM1 excretion above maximum levels of European Union in milk. Among cow breeds, the milk from ‘non-descript’ breed (OR: 11.5) was found to be most contaminated with AFM1 and the least from Jersey breed (OR: 1.0). The present study highlighted the presence of AFM1 in milk samples; therefore, regular monitoring of AFM1 in milk is required so that high risk regions and associated risk factors can be addressed appropriately.

scholarly journals Composition of raw milk from conventional and organic dairy farming

2016 ◽  
Vol 32 (2) ◽  
pp. 133-143 ◽  
Author(s):  
Denis Kucevic ◽  
Snezana Trivunovic ◽  
Vladan Bogdanovic ◽  
Ksenija Cobanovic ◽  
Dobrila Jankovic ◽  
...  
Keyword(s):  
Statistical Significance ◽  
Raw Milk ◽  
Farming System ◽  
Dairy Farming ◽  
Milk Samples ◽  
High Statistical Significance ◽  
Significant Difference ◽  
Organic Dairy ◽  
One Year ◽  
The Impact

Possible differences between composition of raw milk due to dairy farming system (organic vs conventional) as well as seasonal variations were investigated. The samples were analysed during one year. A total of 6.782 samples of raw milk were collected (4.496 from organic farming). Dairy farms were located in the northern part of Republic of Serbia (Province of Vojvodina). The principle of analysis of raw milk samples was in accordance with the methodology by midinfrared spectrometry and flow cytometry. The fixed effect of system of farming and season (winter, spring, summer and fall) have shown a high statistical significance (P < 0.01) on all examined milk parameters except fat, total solids and somatic cell count, where the impact was slightly lower (P < 0.05). Significant difference wasn't found in number of bacterial colonies (P > 0.05). Composition of milk is also affected by a number of other factors, therefore it is recommended to involve factors such as nutrition of dairy cows, breed and farm management.

Nutrient composition of pastoralist's raw milk of different breeds of cattle in Adamawa and Taraba states, Northeastern Nigeria

2020 ◽  
Vol 47 (2) ◽  
pp. 217-223
Author(s):  
Z. Adamu ◽  
M. B. Ardo ◽  
Y. H. Aliyara
Keyword(s):  
Moisture Content ◽  
Local Governments ◽  
Management Practices ◽  
Raw Milk ◽  
Fat Content ◽  
Ash Content ◽  
Milk Samples ◽  
Moisture Contents ◽  
Significant Difference ◽  
Content Range

This study was carried out to determine the proximate composition of raw milk produced in pastoral settlements. Six hundred pastoralists' raw milk samples were collected from 20 local governments in Adamawa and Taraba states, Nigeria. Milk samples were collected from White Fulani (WF), Red Bororo (RB) and Sokoto Gudali (SG) breeds of cattle and were analyzed for protein, fat, ash and moisture contents. The protein content ranged between 3.62±0.38% -3.95±0.11% in WF, 3.29±0.8% - 3.94±0.10% in RB and 3.31±0.27%- 3.95±0.09% in SG in Adamawa and Taraba states. The fat content ranged between 3.55±0.47% - 3.99±0.03% in WF, 3.98±0.04% - 3.98±0.06% in RB and 3.32±0.20% - 3.45±0.27% in SG. The ash content recorded was between 0.40±0.06% -0.41±0.04% in WF, 0.40±0.06% - 0.43±0.07% in RB and0.39±0.06% - 0.41±0.08% in SG, and the moisture content in Adamawa and Taraba states were between 83.52±2.07% - 84.00±0.57% in WF, 82.28±1.05% - 83.73±0.63% in RB and 82.90±1.48% - 83.56±1.35%in SG. The study from the two states revealed protein value between 3.29± 0.8% - 3.95±0.11%, fat content range of3.32±0.20% - 3.99±0.03%, ash content of between 0.39± 0.06% - 0.43±0.07% and moisture content that ranged between 82.28± 1.05% - 84.00±0.57%. Constituents of milkfrom Taraba state were higher in values than those from Adamawa sate. The statistical analysis of the results at95% confidence level showed significant difference among breeds and states. In comparison, the three breeds that resided in Adamawa state had least values, which could be attributed to herd management practices. This study showed that all the three pastoralists' breeds indicated desirable components in their milk Cross breeding with higher breeds and provision of quality feed and water may lead to better yield in all the breeds in this study.

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