scholarly journals Bioprospection and genetic diversity of endophytic bacteria associated with cassava plant

2018 ◽  
Vol 31 (2) ◽  
pp. 315-325 ◽  
Author(s):  
MARIA CAMILA DE BARROS SILVA LEITE ◽  
ARTHUR PRUDÊNCIO DE ARAUJO PEREIRA ◽  
ADIJAILTON JOSÉ DE SOUZA ◽  
FERNANDO DINI ANDREOTE ◽  
FERNANDO JOSÉ FREIRE ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Plant Growth ◽  
Endophytic Bacteria ◽  
Inorganic Phosphate ◽  
Low Fertility ◽  
Rrna Gene ◽  
Bacterial Isolates ◽  
Cassava Plant ◽  
Bacterial Groups ◽  
Sanger Method

ABSTRACT Cassava is mostly planted in sandy soils which are usually of low fertility, thereby making it necessary to perform beneficial associations with microorganisms that can promote their growth. In this perspective, the possibility of selecting bacterial isolates efficient in promoting the growth of the culture is evident, which can provide subsidies for future inoculants. The objective of this study was to isolate, identify, select and evaluate the genetic diversity of endophytic bacteria in roots and stems of cassava grown in Garanhuns - PE, with features involved in promoting plant growth. The isolation was performed on culture medium semisolid LGI-P. The selected isolates were evaluated for the potential to fix N2, as the ability to produce indole acetic acid, for their ability to solubilize inorganic phosphate and produce exopolysaccharides. Some bacterial isolates had their 16S rRNA gene sequenced by the Sanger method. A total of 52 endophytic bacteria isolates were obtained from cassava. Regarding the potential to fix N2, 15% of the isolates were positive. As for the production of IAA, 78% of the isolates produced this phytohormone in a medium with increased L-tryptophan. Approximately 31% of the isolates were able to solubilize inorganic phosphate and 60% had exopolysaccharide. The identification of 19 isolates allowed the grouping into six bacterial genera, namely: Achromobacter, Bacillus, Burkholderia, Enterobacter, Pantoea and Pseudomonas. Cassava plants grown in Garanhuns - PE present interaction with different groups of endophytic bacteria and there are bacterial groups with several characteristics involved in promoting plant growth.

scholarly journals Diversity and biological activity of culturable endophytic bacteria associated with marigold (Calendula officinalis L.)

2021 ◽  
Vol 7 (3) ◽  
pp. 336-353
Author(s):  
Vyacheslav Shurigin ◽  
◽  
Burak Alaylar ◽  
Kakhramon Davranov ◽  
Stephan Wirth ◽  
...  
Keyword(s):  
Plant Growth ◽  
Endophytic Bacteria ◽  
Plant Pathogens ◽  
Hydrolytic Enzymes ◽  
Pathogenic Fungi ◽  
Rrna Gene ◽  
Plant Pathogenic Fungi ◽  
Bacterial Isolates ◽  
Calendula Officinalis ◽  
Fungal Plant Pathogens

<abstract> <p>Endophytes colonizing plant tissue play an essential role in plant growth, development, stress tolerance and plant protection from soil-borne diseases. In this study, we report the diversity of cultivable endophytic bacteria associated with marigold (<italic>Calendula officinalis</italic> L.) by using 16S rRNA gene analysis and their plant beneficial properties. A total of 42 bacterial isolates were obtained from plant tissues of marigold. They belonged to the genera <italic>Pantoea, Enterobacter, Pseudomonas, Achromobacter, Xanthomonas, Rathayibacter, Agrobacterium, Pseudoxanthomonas</italic>, and <italic>Beijerinckia</italic>. Among the bacterial strains, <italic>P. kilonensis</italic> FRT12, and <italic>P. rhizosphaerae</italic> FST5 showed moderate or vigorous inhibition against three tested plant pathogenic fungi, <italic>F. culmorum, F. solani</italic> and <italic>R. solani</italic>. They also demonstrated the capability to produce hydrolytic enzymes and indole-3-acetic acid (IAA). Five out of 16 isolates significantly stimulated shoot and root growth of marigold in a pot experiment. The present study reveals that more than half of the bacterial isolates associated with marigold (<italic>C. officinalis</italic> L.) provided antifungal activity against one or more plant pathogenic fungi. Our findings suggest that medicinal plants with antimicrobial activity could be a source for selecting microbes with antagonistic activity against fungal plant pathogens or with plant growth stimulating potential. These isolates might be considered as promising candidates for the improvement of plant health.</p> </abstract>

scholarly journals Potential of endophytic bacteria from rice root as potassium solvent

2019 ◽  
Vol 20 (5) ◽  
Author(s):  
WARZATULLISNA WARZATULLISNA ◽  
LENNI FITRI ◽  
YULIA SARI ISMAIL
Keyword(s):  
16S Rrna ◽  
Plant Growth ◽  
Endophytic Bacteria ◽  
Oryza Sativa L ◽  
Rice Root ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Rrna Gene ◽  
Bacterial Isolates ◽  
Biochemical Tests

Abstract. Warzatullisna, Fitri L, Ismail YS. 2019. Potential of endophytic bacteria from rice root as potassium solvent. Biodiversitas 20: 1303-1308. Endophytic bacteria live in plant tissues and known to have many benefits for plant growth. One function of endophytic bacteria are known to be able to dissolve potassium. Potassium is one of the macronutrients that plays an important role in plant growth and development. This study aims to obtain endophytic bacterial isolates from the roots of rice plants (Oryza sativa L.) which have potential as potassium solvents, identify the endophytic bacteria in morphologically and physiologically, and analysis of the selected isolate 16S rRNA genes. Selected endophytic bacterial isolates from the test of dissolving potassium on medium Alexandrov, measured clear zone using calipers and continued with biochemical tests using KIT DL-96E also identified based on 16S rRNA gene and compared for their close relationship with reference strains available in the Bank Genes. Based on the isolation results, 7 isolates from the roots of rice were obtained. Six isolates of endophytic bacteria were able to dissolve potassium. EPK3 isolates were the isolates that are capable of dissolving the highest potassium in Alexandrov media with a 17.9 mm potassium dissolution index. EPK3 isolates also produce inhibit the growth of Xanthomonas oryzae with a 1.2 mm inhibition zone. Physiological identification showed that EPK3 isolates were Enterobacter cloaceae bacteria with 93.79% of similarity. Based on the 16SrRNA gene showed that EPK3 isolates were closely related to E. cloaceae strain SBP-8 with similarity rate is 99%.

scholarly journals Biodiversity of major bacterial groups in association with agarwood (Aquilaria crassna) in Khanh Hoa province, Vietnam

2014 ◽  
Vol 6 (2) ◽  
pp. 132-137 ◽  
Author(s):  
Thi Thanh Tra Nguyen ◽  
Van Duy Nguyen
Keyword(s):  
16S Rrna ◽  
Endophytic Bacteria ◽  
Bacillus Pumilus ◽  
Sequence Similarity ◽  
Alcaligenes Faecalis ◽  
Rrna Gene ◽  
Bacterial Isolates ◽  
Group Ii ◽  
Bacterial Groups ◽  
Aquilaria Crassna

Agarwood mainly formed by Aquilaria species is an economically and pharmaceutically important natural product used for the production of incense, perfumes and traditional medicines in Asia. Endophytic bacteria are potentially important in producing pharmaceutical compounds found in the plants. The aim of this research is to isolate, classify and identify major endophytic bacteria groups associated with agarwood of Aquilaria crassna species in Khanh Hoa province, Vietnam. Agarwood samples were collected and surface-sterilized, and total endophytic bacteria were isolated on Tryptic Soy Agar by the spread plate method. Major bacterial groups were classified according to the Bergey’s system. The 16S rRNA gene fragments were amplified using PCR method, and bacterial isolates were identified using this gene sequence similarity based method. The results showed that from 0.121 g of agarwood, total 26 bacterial isolates were purified and divided into 7 separated groups, in which the group II of Gram-positive spore-forming bacteria was the most dominant. Especially, two dominant strains, T14 of group II, and T15 of group VII, were identified as Bacillus pumilus and Alcaligenes faecalis, respectively. To our knowledge, it is the first time that biodiversity of bacterial endophytes associated with agarwood from Aquilaria crassna in Vietnam has been reported, which requires of further study to understand the relationship of endophytic bacteria to agarwood-producing Aquilaria crassna species as well as explore their potential applications towards the development of valuable bioactive compounds. Trầm hương, chủ yếu được tạo ra từ các loài cây Dó (Aquilaria), là một sản phẩm tự nhiên có giá trị kinh tế và y học đã được sử dụng để sản xuất hương, nước hoa và các dược phẩm truyền thống ở châu Á. Vi khuẩn nội cộng sinh thực vật được cho là một nguồn quan trọng cho các dược phẩm có nguồn gốc thực vật. Mục tiêu của nghiên cứu này là nhằm phân lập, phân loại và định danh các nhóm vi khuẩn chính trên Trầm hương Khánh Hòa, Việt Nam. Các mẫu Trầm hương được thu nhận và vô trùng bề mặt dùng để phân lập vi khuẩn tổng số trên môi trường TSA bằng phương pháp trải đĩa. Các nhóm vi khuẩn chính được phân loại dựa theo hệ thống chuẩn Bergey. Đoạn gen mã hóa 16S rRNA được khuếch đại bằng phương pháp PCR, và các chủng vi khuẩn được định danh bằng phép so sánh độ tương đồng trình tự của đoạn gen này. Kết quả cho thấy từ 0,121 g mẫu trầm hương, chúng tôi đã phân lập được 26 chủng vi khuẩn và phân chúng vào 7 nhóm chính, trong đó nhóm II bao gồm các vi khuẩn Gram dương sinh bào tử là nhóm chiếm ưu thế nhất. Đặc biệt, có 2 chủng ưu thế là chủng T14 thuộc nhóm II và chủng T15 thuộc nhóm VII đã được định danh tương ứng là Bacillus pumilus và Alcaligenes faecalis. Đây là nghiên cứu đầu tiên về đa dạng sinh học của các nhóm vi khuẩn chính trên Trầm hương Khánh Hòa. Vì vậy, cần có những nghiên cứu tiếp theo nhằm tìm hiểu mối quan hệ giữa các vi khuẩn nội cộng sinh với cây Dó bầu (Aquilaria crassna) tạo trầm cũng như khai thác những ứng dụng tiềm năng của các vi khuẩn này theo hướng phát triển các hoạt chất sinh học có giá trị.

scholarly journals Diversity and Biocontrol Potential of Cultivable Endophytic Bacteria Associated with Halophytes from the West Aral Sea Basin

2021 ◽  
Vol 9 (7) ◽  
pp. 1448
Author(s):  
Lei Gao ◽  
Jinbiao Ma ◽  
Yonghong Liu ◽  
Yin Huang ◽  
Osama Abdalla Abdelshafy Mohamad ◽  
...  
Keyword(s):  
Endophytic Bacteria ◽  
Diversity Index ◽  
Pathogenic Fungi ◽  
Significant Inhibition ◽  
Isolation Effect ◽  
Aral Sea ◽  
Rrna Gene ◽  
Bacterial Isolates ◽  
Aral Sea Basin ◽  
Soil Borne Pathogens

Endophytes associated with halophytes may contribute to the host’s adaptation to adverse environmental conditions through improving their stress tolerance and protecting them from various soil-borne pathogens. In this study, the diversity and antifungal activity of endophytic bacteria associated with halophytic samples growing on the shore of the western Aral Sea in Uzbekistan were investigated. The endophytic bacteria were isolated from the nine halophytic samples by using the culture-dependent method and identified according to their 16S rRNA gene sequences. The screening of endophytic bacterial isolates with the ability to inhibit pathogenic fungi was completed by the plate confrontation method. A total of 289 endophytic bacterial isolates were isolated from the nine halophytes, and they belong to Firmicutes, Actinobacteria, and Proteobacteria. The predominant genera of the isolated endophytic bacteria were Bacillus, Staphylococcus, and Streptomyces, accounting for 38.5%, 24.7%, and 12.5% of the total number of isolates, respectively. The comparative analysis indicated that the isolation effect was better for the sample S8, with the highest diversity and richness indices. The diversity index of the sample S7 was the lowest, while the richness index of samples S5 and S6 was the lowest. By comparing the isolation effect of 12 different media, it was found that the M7 medium had the best performance for isolating endophytic bacteria associated with halophytes in the western Aral Sea Basin. In addition, the results showed that only a few isolates have the ability to produce ex-enzymes, and eight and four endophytic bacterial isolates exhibited significant inhibition to the growth of Valsa mali and Verticillium dahlia, respectively. The results of this study indicated that halophytes are an important source for the selection of microbes that may protect plant from soil-borne pathogens.

Assessing the Plant Growth-promoting Traits and Host Specificity of Endophytic Bacteria of Pulse Crops

2021 ◽  
Author(s):  
R. Thamizh Vendan ◽  
D. Balachandar
Keyword(s):  
Plant Growth ◽  
Endophytic Bacteria ◽  
Gene Sequencing ◽  
16S Rrna Gene Sequencing ◽  
Growth And Yield ◽  
Rrna Gene ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Rrna Gene Sequencing ◽  
Plant Growth Promoting Traits

Background: Symbiotic associations between legumes and Rhizobia are ancient and fundamental. However, the plant growth-promoting endophytes other than Rhizobia are not yet fully explored for pulses productivity. The present study was aimed to isolate efficient endophytic bacteria from pulses, assess their diversity, screen their plant growth-promoting activities and to test their potential as bio inoculants for pulses.Methods: We have isolated several endophytic bacteria from pulse crops more specifically from blackgram (Vigna mungo) and greengram (Vigna radiata). After careful screening, 15 promising endophytic isolates were selected for this study. The identification of endophytic bacterial isolates was performed by 16S rRNA gene sequencing. The isolates were tested for their potential for the plant growth-promoting traits such as nitrogen fixation, phosphate solubilization, indole-3-acetic acid production, siderophore secretion and antifungal activity. Pot culture experiments were conducted with the screened potential endophytic cultures.Result: The 16S rRNA gene sequencing revealed that species of Enterobacter, Bacillus, Pantoea, Pseudomonas, Acromobacter, Ocrobacterium were found as endophytes in blackgram and greengram. The in vitro screening identified Bacillus pumilus (BG-E6), Pseudomonas fluorescens (BG-E5) and Bacillus licheniformis (BG-E3) from blackgram and Pseudomonas chlororaphis (GG-E2) and Bacillus thuringiensis (GG-E7) from greengram as potential plant growth-promoting endophytes. These strains showed antagonism against plant pathogenic fungi. Upon inoculation of these endophytic PGPR strains, the blackgram and greengram growth and yield got increased. Among the strains, BG-E6 recorded 14.7% increased yield in blackgram and GG-E2 accounted for a 19.5% yield increase in greengram compared to respective uninoculated control. The experimental results showed that there was a host specificity found among the endophytic bacterial cultures with pulses. The cross inoculation of endophytic strains did not perform well to enhance the growth and yield of their alternate hosts. 

scholarly journals Evaluation of the plant-growth-promoting abilities of endophytic bacteria from the psammophyteAmmodendron bifolium

2018 ◽  
Vol 64 (4) ◽  
pp. 253-264 ◽  
Author(s):  
Yanlei Zhu ◽  
Xiaoping She
Keyword(s):  
Seed Germination ◽  
Plant Growth ◽  
Endophytic Bacteria ◽  
Growth Stages ◽  
Bacterial Isolates ◽  
Pellicle Formation ◽  
Radicle Growth ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Plant Growth Promoting Traits

The objective of this study was to assess the plant-growth-promoting abilities of 45 endophytic bacterial isolates from Ammodendron bifolium through physiological characteristics detection and endophytic bacteria–plant interaction. Each of these isolates exhibited 1 or more plant-growth-promoting traits, but only 11 isolates belonging to the genera Bacillus, Staphylococcus, and Kocuria were capable of promoting seed germination and radicle growth. These results together with the results of the correlation analysis revealed that the completion of seed germination may not be due to IAA production, phosphate solubilization, pellicle formation, and ACC deaminase, protease and lipase production by endophytic bacteria, but may be closely related to amylase and cellulase production. Further, endophytic bacterial isolates with plant-growth-promoting traits may also provide beneficial effects to host plants at different growth stages. Thus, these results are of value for understanding the ecological roles of endophytic bacteria in host plant habitats and can serve as a foundation for further studies of their potential in plant regeneration.

scholarly journals Isolation and characterization of plant growth-promoting endophytic bacteria of wild legumes growing on sandy soils of Binh Thuan province, Vietnam

2021 ◽  
Vol 10 (3) ◽  
pp. 246-254
Author(s):  
Dang Thi Ngoc Thanh ◽  
Pham Thi Thu Ly ◽  
Pham Thi Nga ◽  
Pham Van Ngot
Keyword(s):  
Nitrogen Fixation ◽  
Plant Growth ◽  
Endophytic Bacteria ◽  
Phosphate Solubilization ◽  
Sandy Soils ◽  
Legume Species ◽  
Bacterial Isolates ◽  
Isolation And Characterization ◽  
Plant Growth Promoting ◽  
Growth Promoting

The roots of two legume species (Tephrosia purpurea and Tephrosia villosa) that grew wild on dry sandy soils of Binh Thuan province were sources for isolating plant growth-promoting endophytic bacteria. Semi-solid LGI medium was used for the isolation of nitrogen-fixing bacteria from root extracts. All bacterial isolates isolates were evaluated for their ability to solubilize calcium orthophosphate on solid NBRIP medium and their ability to produce IAA in Burk's liquid medium supplemented with 100 mg/L tryptophan. The possibilities of nitrogen fixation, phosphate solubilization and IAA synthesis were all quantitative examined by colorimetric method. Twenty-two bacterial isolates of T. purpurea and 18 isolates of T. villosa were capable of nitrogen fixation in the range of 1.94 to 2.81 mg/L NH4+, whereas only 18 isolates of T. purpurea and 16 isolates of T. villosa showed phosphate solubilization in the range of 12.30 – 48.90 mg/L P2O5, and IAA production in the range of 0.38 – 12.72 mg/L. Sixteen outstanding bacterial isolates of the two legume species were identified by MALDI-TOF technique. The results showed that 13 isolates had high similarity with five bacterial genera including Klebsiella, Cronobacter, Enterobacter, Burkholderia, and Bacillus with score values in the range of 2.070 – 2.411.

scholarly journals Salix purpurea and Eleocharis obtusa Rhizospheres Harbor a Diverse Rhizospheric Bacterial Community Characterized by Hydrocarbons Degradation Potentials and Plant Growth-Promoting Properties

Plants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1987
Author(s):  
Fahad Alotaibi ◽  
Soon-Jae Lee ◽  
Marc St-Arnaud ◽  
Mohamed Hijri
Keyword(s):  
Carbon Source ◽  
Plant Growth ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Petrochemical Plant ◽  
Bacterial Isolates ◽  
Pgp Traits ◽  
Degrading Bacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting

Phytoremediation, a method of phytomanagement using the plant holobiont to clean up polluted soils, is particularly effective for degrading organic pollutants. However, the respective contributions of host plants and their associated microbiota within the holobiont to the efficiency of phytoremediation is poorly understood. The identification of plant-associated bacteria capable of efficiently utilizing these compounds as a carbon source while stimulating plant-growth is a keystone for phytomanagement engineering. In this study, we sampled the rhizosphere and the surrounding bulk soil of Salixpurpurea and Eleocharis obusta from the site of a former petrochemical plant in Varennes, QC, Canada. Our objectives were to: (i) isolate and identify indigenous bacteria inhabiting these biotopes; (ii) assess the ability of isolated bacteria to utilize alkanes and polycyclic aromatic hydrocarbons (PAHS) as the sole carbon source, and (iii) determine the plant growth-promoting (PGP) potential of the isolates using five key traits. A total of 438 morphologically different bacterial isolates were obtained, purified, preserved and identified through PCR and 16S rRNA gene sequencing. Identified isolates represent 62 genera. Approximately, 32% of bacterial isolates were able to utilize all five different hydrocarbons compounds. Additionally, 5% of tested isolates belonging to genera Pseudomonas, Acinetobacter, Serratia, Klebsiella, Microbacterium, Bacillus and Stenotrophomonas possessed all five of the tested PGP functional traits. This culture collection of diverse, petroleum-hydrocarbon degrading bacteria, with multiple PGP traits, represents a valuable resource for future use in environmental bio- and phyto-technology applications.

scholarly journals Plant growth-promoting activity in bean plants of endophytic bacteria isolated from Echeveria laui

2021 ◽  
Vol 5 (2) ◽  
pp. 65
Author(s):  
Anderson Emmer ◽  
João Arthur Dos Santos Oliveira ◽  
Andressa Domingos Polli ◽  
Julio Cesar Polonio ◽  
Leonardo Hamamura Alves ◽  
...  
Keyword(s):  
Plant Growth ◽  
Endophytic Bacteria ◽  
Arid Environment ◽  
Bacterial Isolates ◽  
Phaseolus Vulgaris L ◽  
Bean Plants ◽  
Plant Growth Promoting ◽  
Plant Growth Promoting Activity ◽  
Growth Promoting

Echeveria laui (Crassulaceae) is commonly commercialized due to its drought-tolerance capacity and to its rosette-shaped aesthetics. Since endophytes associated with plants from a dry or arid environment have scarcely been analyzed as yet, current research comprises the isolation of leaf endophytic bacteria from E. laui (one five-year-old and one two-year-old plants) investigating plant growth-promoting endophytic bacteria which may solubilize phosphate, fix nitrogen, produce exopolysaccharides/IAA and antagonize phytopathogens. Isolation by the maceration methodology provided a colonization rate of 1.98 x109 CFU g-1 for the two-year-old plant and 1.14 x 1010 CFU g-1 for the five-year-old one. All 40 isolates evaluated showed in vitro plant growth-promoting agent’s abilities, with emphasis on EG04, ELG18, and ELP06.  The capacity of the three best bacterial isolates were evaluated under greenhouse conditions in common and black bean (Phaseolus vulgaris L.) plants. Based on the sequencing of the 16S rRNA region and phylogenetic analysis, the three endophytes were identified as Pantoea sp. (ELG04 and ELG18) and Erwinia sp. (ELP06). Under greenhouse conditions, statistically significant differences were found among the plants treated with the three endophytes when compared to control plants for fresh and dry shoot, root biomass and length.

scholarly journals Potensi Bakteri Endofit dari Tanaman Sagu (Metroxylon spp.) sebagai Agens Pemacu Pertumbuhan Tanaman Padi

2018 ◽  
Vol 14 (2) ◽  
pp. 75-80
Author(s):  
Yatni Yatni ◽  
Gratiana N C Tuhumury ◽  
Christoffol Leiwakabessy
Keyword(s):  
Plant Growth ◽  
Endophytic Bacteria ◽  
Growth Promoter ◽  
Plant Growth Promoting Bacteria ◽  
Test Results ◽  
Bacterial Isolates ◽  
The People ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Stems And Leaves

Sago is a staple plant of the people of Maluku and Papua, which have many benefits and advantages to continue to be developed. Endophytic bacteria are bacteria that live in plant tissues and colonize the intercellular and vascular systems. This study aims to obtain endophytic bacterial isolates from parts of the roots, stems, and leaves of sago plants which have the potential as agents for plant growth promoting bacteria. Endophytic bacteria are isolated from the roots, stems, and leaves. Based on the results of isolation, 21 isolates of endophytic bacteria were found. Then, the selection is done by hypersensitivity test and gram reaction test. The results of the selection obtained 20 isolates of endophytic bacteria that were not pathogenic. After that, it was followed by plant growth promoting test for endophytic bacterial isolates. The test results were obtained three isolates potentially as plant growth promoter that is STA1, STA6, and STA11. Keywords: endophytic bacteria, plant growth promoting, sago   ABSTRAK Tanaman sagu merupakan tanaman pokok masyarakat Maluku dan Papua, yang memiliki banyak manfaat dan keunggulan untuk terus dikembangkan. Bakteri endofit adalah bakteri yang hidup di dalam jaringan tanaman dan berkoloni pada daerah ruang interseluler dan sistem vascular. Penelitian ini bertujuan untuk mendapatkan isolat bakteri endofit dari bagian akar,batang dan daun tanaman sagu yang berpotensi sebagai agens pemacu pertumbuhan tanaman. Bakteri endofit diisolasi dari bagian akar, batang, dan daun. Berdasarkan hasil isolasi yang telah dilakukan didapati 21 isolat bakteri endofit. Kemudian seleksi dilakukan dengan uji hipersensitif dan uji reaksi gram. Hasil seleksi diperoleh 20 isolat bakteri endofit yang bukan patogen. Setelah itu dilanjutkan dengan uji pemacu pertumbuhan tanaman terhadap isolat bakteri endofit. Hasil pengujian tersebut diperoleh tiga isolat bakteri yang berpotensi sebagai pemacu pertumbuhan tanaman yaitu STA1, STA6, dan STA11. Kata kunci: bakteri endofit, pemacu pertumbuhan tanaman, sagu

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