scholarly journals EVALUATION OF VIABILITY OF Tabebuia aurea SEEDS THROUGH TETRAZOLIUM TEST

2020 ◽  
Vol 33 (4) ◽  
pp. 993-999
Author(s):  
WASHINGTON APARECIDO DA LUZ BRITO ◽  
KLEANE TARGINO OLIVEIRA PEREIRA ◽  
NARJARA WALESSA NOGUEIRA ◽  
SALVADOR BARROS TORRES ◽  
EMANOELA PEREIRA DE PAIVA
Keyword(s):  
Experimental Design ◽  
Evaluation Method ◽  
Reliable Information ◽  
Test Results ◽  
Germination Test ◽  
Test Taking ◽  
Test Methodology ◽  
Tetrazolium Test

ABSTRACT The viability of T. aurea seeds is evaluated through the germination test, taking 21 days, which makes it difficult to obtain quick information about the viability of its seeds. In this context, using the tetrazolium test as an evaluation method would be appropriate because it provides faster and more reliable information for several species. In view of the above, the objective of this study was to adapt the tetrazolium test methodology to evaluate the viability of T. aurea seeds. Initially, the imbibition curve was constructed to determine the appropriate hydration period for the seeds and the germination test was conducted, both for evaluating the initial quality and for comparison with the tetrazolium test results. For the tetrazolium test, the seeds were hydrated for 24 hours, then their coats were removed and the seeds were immersed in tetrazolium solutions. The concentrations used were 0.05, 0.075 and 0.1% for three staining periods 2, 4 and 6 hours, at 35 and 40 °C, in the absence of light. The experimental design was completely randomized in a 3 x 3 + 1 factorial scheme (three concentrations of tetrazolium solution x three staining periods + one control = germination test) with four replicates of 25 seeds. Tetrazolium test was adequate to evaluate the viability of T. aurea seeds using the concentration of 0.05% for four hours at 40 °C.

scholarly journals Improvement of the methodology of the tetrazolium test using different pretreatments in seeds of the genus Epidendrum (Orchidaceae)

2020 ◽  
Vol 42 ◽  
Author(s):  
Seir Antonio Salazar Mercado ◽  
Jesús David Quintero Caleño ◽  
Laura Yolima Moreno Rozo
Keyword(s):  
Deionized Water ◽  
Control Group ◽  
Distilled Water ◽  
In Vitro Germination ◽  
Test Results ◽  
Germination Test ◽  
Viability Test ◽  
Cloth Filter ◽  
Tetrazolium Test

Abstract: The aim of the present study was to determine the most suitable pretreatment to enhance the tetrazolium test in seeds of the Epidendrum genus. Initially, mature capsules were harvested at El Escorial village, in the municipality of Pamplona, Colombia. Subsequently, the seeds were subjected to five pretreatments: deionized water, 0.5% NaClO, 1.0% NaClO, 10.0% sucrose and a control group. Using the syringe method with cloth filter, the seeds were rinsed with distilled water and subjected to two concentrations of tetrazolium solution (0.25%, 1.0%) and three exposure times (6 h, 24 h and 48 h). Finally, the tetrazolium viability test results were correlated with the in vitro germination test. It was found that the use of deionized water improves the efficiency of the tetrazolium test in seeds of Epidendrum fimbriatum and E. microtum; as in seeds of E. elongatum when using 1.0% tetrazolium for 24 h. Similarly, a high correlation was found between viability and germination, using deionized water and 10.0% sucrose, with homogeneous results with each other.

scholarly journals Tetrazolium test for assessment of seed viability of atemoya (Annona cherimola Mill. x A. squamosa L.)

2014 ◽  
Vol 36 (3) ◽  
pp. 357-361 ◽  
Author(s):  
Juliana Iassia Gimenez ◽  
Gisela Ferreira ◽  
Claudio Cavariani
Keyword(s):  
Experimental Design ◽  
Seed Viability ◽  
Tetrazolium Salt ◽  
Germination Test ◽  
Tissue Integrity ◽  
Annona Cherimola ◽  
The Family ◽  
Viable Seeds ◽  
Tetrazolium Test

The study aimed to adapt the tetrazolium test to assess the viability of atemoya (Annona cherimola Mill. x A. squamosa L.) seeds. The experimental design was completely randomized in 3 x 3 factorial arrangement (tetrazolium salt concentrations x exposure times), totaling 9 treatments with four replicates of 25 seeds. After immersion in water for 24 hours, the seeds were longitudinally sectioned and exposed to three tetrazolium salt concentrations (0.05%, 0.5% and 1%) for three periods of time (2, 4 and 6 hours). The evaluations were performed according to pre-established criteria of tissue integrity and staining. The germination test was performed, using the GA 4+7 + N-(fenilmetil)-aminopurina regulators at concentrations of 0 and 300 mg.L-1, because dormancy is observed in species of the family Annonaceae. Tetrazolium salt at 1% yielded the highest percentage of viable seeds within the shortest time, while longer exposure times were necessary with concentrations of 0.05% and 0.5%. It can be concluded that the concentration of 1% of tetrazolium salt for two hours of exposure is more appropriate for assessing the viability of atemoya seeds.

scholarly journals Suitability of the tetrazolium test methodology for recently harvested and stored coffee seeds

2012 ◽  
Vol 36 (4) ◽  
pp. 415-423 ◽  
Author(s):  
Aline da Consolação Sampaio Clemente ◽  
Maria Laene Moreira de Carvalho ◽  
Renato Mendes Guimarães
Keyword(s):  
Sodium Hypochlorite ◽  
Second Phase ◽  
Test Results ◽  
Manual Removal ◽  
Test Methodology ◽  
Negative Effect ◽  
Uniform Manner ◽  
Two Stages ◽  
Manual Extraction ◽  
Tetrazolium Test

Germination of coffee seeds occurs in a slow and non-uniform manner and the tetrazolium test (TZ) may be a quick alternative for assessment of viability, as long as the preparation and imbibition of the seeds is established. This study was performed in two stages: in the first, recently harvested seeds from three coffee cultivars were submitted to four methodologies for removal of the parchment: manual extraction or use of sodium hypochlorite (NaClO at 5%) and two periods of imbibition of the seeds in water (36 and 48 hours) for removal of the embryos. In the second phase, manual removal and the use of NaClO were compared for elimination of the parchment of stored seeds for monthly performance of the tetrazolium test for six months. Sodium hypochlorite is not efficient in total extraction of the parchment and has a negative effect on the results of the TZ test on low moisture coffee seeds. An imbibition time of 48 hours facilitates extraction of embryos and does not affect test results. The tetrazolium test on coffee seeds with different moisture and deterioration levels must be undertaken with manual removal of the parchments.

scholarly journals Viability of Libidibia ferrea (Mart. ex Tul.) L.P. Queiroz var. ferrea) seeds by tetrazolium test

2017 ◽  
Vol 39 (1) ◽  
pp. 7-12 ◽  
Author(s):  
Sara Monaliza Costa Carvalho ◽  
Salvador Barros Torres ◽  
Clarisse Pereira Benedito ◽  
Narjara Walessa Nogueira ◽  
Antonia Adailha Torres Souza ◽  
...  
Keyword(s):  
Experimental Design ◽  
Factorial Design ◽  
Germination Test ◽  
Rapid Tests ◽  
Viable Seeds ◽  
Dunnett's Test ◽  
Tetrazolium Test

Abstract: Rapid tests have been essential to evaluate the physiological potential of seeds and the tetrazolium test is one of those which have been used by seed companies. The objective of the study was to establish the procedure for the tetrazolium test in Libidibia ferrea (Mart. Ex Tul.) L.P. Queiroz var. ferrea seeds. For this, three tetrazolium solution concentrations (0.05, 0.075 and 0.1%) and three staining periods (1, 3 and 6 hours) at temperatures of 35 to 40 °C were tested. The seeds were also evaluated by the germination test, whose result was compared with viability by the tetrazolium test. The experimental design was completely randomized in a factorial design 3 x 3 + 1 (three concentrations x three periods + a control = germination test) for each temperature. Viable seeds in the tetrazolium test were compared by Tukey’s test (p ≤ 0.05), while the comparison between the viable seeds with germination test was carried out by Dunnett’s test (p ≤ 0.05). It is recommended that the tetrazolium test for L. ferrea is carried out at a concentration of 0.05% for three-hour staining under 35 °C or 40 °C.

scholarly journals Tetrazolium test for viability estimation of Eugenia involucrata DC. and Eugenia pyriformis Cambess. seeds

2014 ◽  
Vol 36 (3) ◽  
pp. 305-311 ◽  
Author(s):  
Fernanda Bernardo Cripa ◽  
Laura Cristiane Nascimento de Freitas ◽  
Andrieli Cristine Grings ◽  
Michele Fernanda Bortolini
Keyword(s):  
Seed Coat ◽  
Immersion Time ◽  
Preparation Method ◽  
Distilled Water ◽  
Test Results ◽  
Preparation Methods ◽  
Test Methodology ◽  
Time Periods ◽  
Tetrazolium Test

The study aimed to adapt the tetrazolium test methodology to assess the viability of seed of Eugenia involucrata DC. (cherry) and E. pyriformis Cambess. (uvaia) freshly harvested and stored. Three lots of seeds of both species were used, being I (freshly harvested), II (stored for 15 days) and III (stored for 30 days). Seeds (lot I) of both species were immersed in distilled water for 24 hours and submitted to four preparation methods: whole seeds without seed coat and with ¾ of seed coat; longitudinally cut with and without seed coat (immersed in 0.1% tetrazolium for 4 hours). The most suitable preparation method was applied to the remaining lots, where three tetrazolium concentrations were tested: 0.075, 0.1 and 0.5% and three immersion time periods: 2, 4 and 6 hours. The tetrazolium test results were compared to the germination results. For the conduction of the tetrazolium test in seeds of both species we recommend soaking in distilled water by 24 hours and cut lengthwise on seeds with the seed coat. For cherry, the seeds should be immersed in 0.5% tetrazolium solution for 2 hours. For uvaia seeds, additional studies are recommended.

scholarly journals PHYSIOLOGICAL POTENTIAL AND CONSERVATION OF MURUCI (Byrsonima crassifolia) SEEDS

2017 ◽  
Vol 39 (5) ◽  
Author(s):  
CAMILA RIBEIRO DE SOUZA GRZYBOWSKI ◽  
WALNICE MARIA OLIVEIRA DO NASCIMENTO ◽  
ROSEMEIRE CARVALHO DA SILVA ◽  
ELISA SERRA NEGRA VIEIRA ◽  
MARISTELA PANOBIANCO
Keyword(s):  
Block Design ◽  
Rapid Assessment ◽  
Germplasm Collection ◽  
Germination Test ◽  
Byrsonima Crassifolia ◽  
Randomized Block Design ◽  
Relative Air Humidity ◽  
Different Temperatures ◽  
Mother Plants ◽  
Tetrazolium Test

ABSTRACT The goal of the present study is to assess the physiological potential of Byrsonima crassifoliaseeds and analyze the procedures of the germination and tetrazolium tests, as well as the conservation of such seeds by testing different combinations of packaging and environment. The experiment used pyrenes of the clone Açu, collected from mother plants in the germplasm collection of muruci plants of Embrapa Amazonia Oriental, in Belém, Brazil. Different temperatures were tested in the germination test (25, 30, 35 and 20 - 30 ºC). The best methodology and assessment dates were determined for the test, and the seedlings of the species were characterized. Viability was assessed with the tetrazolium test, with combinations of hydration methods (by immersion and between paper towels), staining periods (3 and 4 h) and concentrations of tetrazolium solution (0.5, 0.7 and 1.0%). Storage of muruci pyrenes was evaluated at three, six and twelve months, in polyethylene and Kraft paper bags, under dry chamber and refrigerator conditions. The study used a randomized block design with four replicates. The data underwent analysis of variance. In conclusion, the physiological potential of seeds of B. crassifoliacan be assessed by the germination test conducted in sand, with alternating temperatures of 20-30 ºC, or with the use of the tetrazolium test (with staining for three hours in the tetrazolium solution at 1.0%) as a rapid assessment of viability. Muruci pyrenes can be kept in polythene or paper Kraft bags for up to 12 months, in a dry chamber at 16 ºC and with relative air humidity of 50 to 60%.

scholarly journals Adjustment of the methodology of the tetrazolium test for estimating viability of Eugenia uniflora L. seeds during storage

2014 ◽  
Vol 36 (3) ◽  
pp. 344-351 ◽  
Author(s):  
Daiana Karoline Kaiser ◽  
Laura Cristiane Nascimento de Freitas ◽  
Rubia Priscila Biron ◽  
Simone Cristina Simonato ◽  
Michele Fernanda Bortolini
Keyword(s):  
Seed Viability ◽  
Germination Test ◽  
Preparation Methods ◽  
Eugenia Uniflora ◽  
Paper Towel ◽  
Plastic Bags ◽  
Longitudinal Cutting ◽  
Direct Immersion ◽  
Tetrazolium Test

The study aimed to adjust the methodology of the tetrazolium test to estimate seed viability of Eugenia uniflora L. (Surinam Cherry). Seeds were collected in September 2012 and divided into four lots: freshly harvested (Lot I); stored in plastic bags in a refrigerator at 10 °C for: 15 days (Lot II); 30 days (Lot III); and 45 days (Lot IV). The freshly harvested seeds were preconditioned with direct immersion in water and wet paper towel, followed or not by longitudinal cutting. The seeds were immersed in a 0.1% tetrazolium solution for 4 hours at 30 °C. Appropriate soaking and preparation methods were applied to the seeds lots using three tetrazolium concentrations: 0.1; 0.5; and 1.0%; and four preconditioning periods (2, 4, 6 and 8 hours) at 30 °C. The viability results obtained by the tetrazolium test were compared with those of the germination test. Direct immersion of seeds in water for 24 hours at 25 °C, followed by a longitudinal cut was efficient for preconditioning the seeds. Seed staining with tetrazolium solution at a concentration of 0.5% for 2 hours at 30 °C can be used to estimate the viability of freshly harvested and stored Surinam Cherry seeds.

Development of 2012 Edition of JSME Code for Design and Construction of Fast Reactors: (4) Creep–Fatigue Evaluation Method for Modified 9Cr–1Mo Steel

2013 ◽  
Author(s):  
Shigeru Takaya ◽  
Yuji Nagae ◽  
Tai Asayama
Keyword(s):  
Evaluation Method ◽  
Time To Failure ◽  
Test Results ◽  
Fast Reactors ◽  
Material Test ◽  
Creep Fatigue ◽  
Summation Rule ◽  
Fatigue Evaluation ◽  
Failure Life ◽  
Design And Construction

This paper describes a creep–fatigue evaluation method for modified 9Cr–1Mo steel, which has been newly included in the 2012 edition of the JSME code for design and construction of fast reactors. In this method, creep and fatigue damages are evaluated on the basis of Miner’s rule and the time fraction rule, respectively, and the linear summation rule is employed as the failure criterion. Investigations using material test results are conducted, which show that the time fraction approach can conservatively predict failure life if margins on the initial stress of relaxation and the stress relaxation rate are embedded. In addition, the conservatism of prediction tends to increase with time to failure. Comparison with the modified ductility exhaustion method, which is known to have good failure life predictability in material test results, shows that the time fraction approach predicts failure lives to be shorter in long-term strain hold conditions, where material test data is hardly obtained. These results confirm that the creep–fatigue evaluation method in the code has implicit conservatism.

scholarly journals Use of Game-Based Guidance to Increase Career Awareness of Street Children

2019 ◽  
Vol 6 (2) ◽  
pp. 125-132
Author(s):  
Elia Firda Mufidah
Keyword(s):  
Experimental Design ◽  
Research Method ◽  
Street Children ◽  
Drop Out ◽  
Test Results ◽  
Career Awareness ◽  
Guiding Principles ◽  
Out Of School

Street children are still a problem that needs to be moved. There are still many street children who drop out of school and do not understand what is related to the work taken. Game-based tutoring is in accordance with guiding principles and games so that children can be interested in joining this service. This research method uses pretest-posttest one group pre-experimental design. This study aims to find out how to use this program. This program aims to increase awareness. The Wilcoxon Career Awareness Test Results were obtained by Asymp. Sig = 0.043. Asymp comparison. Sig = 0.043 <α = 0.05 then Ho is accepted and H1 is rejected. Associated with a significant priority on the career awareness scores of street children before the implementation of assistance based on street children's awareness planning. The results of this study are increased career awareness of street children

scholarly journals PENGARUH PSIKOEDUKASI TERHADAP PENGETAHUAN INKLUSIVITAS PADA ANAK-ANAK DI YAYASAN PANTI ASUHAN Z

2021 ◽  
pp. 1429
Author(s):  
Agnes Nugrawati Salim ◽  
Atalya Debora ◽  
Farica Tanojo ◽  
Sriana Sihombing ◽  
Sylvia Dewi Suryaganda ◽  
...  
Keyword(s):  
Social Interaction ◽  
Experimental Design ◽  
Statistical Test ◽  
Test Results ◽  
Significant Difference ◽  
Post Test ◽  
Parametric Statistical Test ◽  
Age Range ◽  
Non Parametric

The research explored the effect of psychoeducation towards knowledge of inclusivity for children at Yayasan Panti Asuhan Z (Z orphanage). Yayasan Panti Asuhan Z (Z orphanage) has been chosen because there is a phenomenon where the typical children shows stigmatization towards the atypical children. There are various form of stigmatization, including physical, attitude, communication, social interaction, et cetera. This research involved 33 children at the Yayasan Panti Asuhan Z having an age range from 11-21 years old. The design used in this research is within-group experimental design. The result of this study indicates that there is an increase after being given psychoeducation. Researchers used the Mann-Whitney non parametric statistical test. The result has shown there is a significant difference between pre-test and post-test results (p < 0.05, N=28).Penelitian ini melihat pengaruh psikoedukasi terhadap pengetahuan inklusivitas pada anak-anak di Yayasan Panti Asuhan Z. Yayasan Panti Asuhan Z dipilih karena adanya fenomena stigmatisasi yang tidak disadari antara anak-anak tipikal terhadap anak-anak atipikal yang tinggal di panti tersebut. Bentuk stigmatisasi yang terjadi beragam, seperti fisik, sikap, cara berkomunikasi, cara berteman, dan sebagainya. Penelitian ini melibatkan 33 anak-anak di Yayasan Panti Asuhan Z yang berusia mulai dari 11 hingga 21 tahun. Desain yang digunakan pada penelitian ini adalah within-group experimental design. Hasil dari penelitian ini menunjukkan bahwa terdapat peningkatan pengetahuan inklusivitas yang ditandai dengan meningkatnya hasil pre-test setelah diberikan psikoedukasi. Peneliti menggunakan uji statistik non-parametrik Mann Whitney. Berdasarkan hasil uji beda tersebut, diketahui bahwa hasil pre-test dan post-test memiliki perbedaan yang signifikan (p < 0.05, N=28).

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