scholarly journals Seed viability test of orchids native to the Brazilian Savanna1

2021 ◽  
Vol 51 ◽  
Author(s):  
Jackeline Schultz Soares ◽  
José Carlos Sorgato ◽  
Luan Marlon Ribeiro ◽  
Jéssica Mônico Celeste Ramos
Keyword(s):  
Ambient Temperature ◽  
Sucrose Solution ◽  
Seed Viability ◽  
Control Treatment ◽  
Essential Factor ◽  
Medicinal Species ◽  
Viability Test ◽  
Orchid Seed ◽  
Viable Seeds ◽  
Tetrazolium Test

ABSTRACT An essential factor for the formation of a native orchid seed bank is the identification of the viability of stored seeds. This study aimed to determine a methodology for optimizing the tetrazolium test, in the identification of the viability of stored seeds of two Orchidaceae medicinal species native to the Brazilian Savanna. Seeds of Miltonia flavescens Lindl. and Schomburgkia crispa Lindl. were submitted to three pre-conditioning conditions: no soaking (control), sucrose solution or distilled water. The seeds were then submitted to a tetrazolium solution, with three types of conditioning: oven, water bath or ambient temperature. The M. flavescens seeds showed a germination of 86.91 %, with a higher percentage of viable seeds (62.32 %) when submitted to pre-conditioning with sucrose + oven conditioning. For S. crispa, the germination was 97.78 %, with a higher percentage of viable seeds for the control treatment + ambient temperature (89.49 %). These results suggest that specific protocols should be used to conduct the tetrazolium test in Orchidaceae. Moreover, when performed on a sample basis, the tetrazolium test should only be used to indicate the seed viability.

scholarly journals Tetrazolium test for assessment of seed viability of atemoya (Annona cherimola Mill. x A. squamosa L.)

2014 ◽  
Vol 36 (3) ◽  
pp. 357-361 ◽  
Author(s):  
Juliana Iassia Gimenez ◽  
Gisela Ferreira ◽  
Claudio Cavariani
Keyword(s):  
Experimental Design ◽  
Seed Viability ◽  
Tetrazolium Salt ◽  
Germination Test ◽  
Tissue Integrity ◽  
Annona Cherimola ◽  
The Family ◽  
Viable Seeds ◽  
Tetrazolium Test

The study aimed to adapt the tetrazolium test to assess the viability of atemoya (Annona cherimola Mill. x A. squamosa L.) seeds. The experimental design was completely randomized in 3 x 3 factorial arrangement (tetrazolium salt concentrations x exposure times), totaling 9 treatments with four replicates of 25 seeds. After immersion in water for 24 hours, the seeds were longitudinally sectioned and exposed to three tetrazolium salt concentrations (0.05%, 0.5% and 1%) for three periods of time (2, 4 and 6 hours). The evaluations were performed according to pre-established criteria of tissue integrity and staining. The germination test was performed, using the GA 4+7 + N-(fenilmetil)-aminopurina regulators at concentrations of 0 and 300 mg.L-1, because dormancy is observed in species of the family Annonaceae. Tetrazolium salt at 1% yielded the highest percentage of viable seeds within the shortest time, while longer exposure times were necessary with concentrations of 0.05% and 0.5%. It can be concluded that the concentration of 1% of tetrazolium salt for two hours of exposure is more appropriate for assessing the viability of atemoya seeds.

scholarly journals VIABILITY AND GERMINATION TESTS OF FRINGED GENTIAN, GENTIANA THERMALIS (O. KUNTZE) ILTIS

HortScience ◽  
1992 ◽  
Vol 27 (12) ◽  
pp. 1262d-1262
Author(s):  
Joan Cochrane ◽  
Harrison Hughes ◽  
James D. Zizz
Keyword(s):  
Seed Viability ◽  
Limited Attention ◽  
Viability Test ◽  
Number Of Seeds ◽  
Tetrazolium Test

Fringed gentian, Gentiana thermalis (O. Kuntze) Iltis is an attractive wildflower, that may be used as a fall flowering ornamental. This species has received limited attention in terms of seed viability and germination. Seeds were subjected to a tetrazolium viability test that involved imbibition for 24 hours at 22C. No embryos in any line tested were stained. However, imbibition at 3C for up to 4 weeks followed by staining at 22C resulted in significant levels of positive viability, as indicated by embryo staining. A germination study indicated that stratification was necessary for germination, with 6 weeks chilling giving better results than 0 or 3 weeks. However, the number of seeds germinated was less than expected, given the results of the tetrazolium test.

Suitability of forms of preparing seeds of Dalbergia miscolobium Benth for exposure to the tetrazolium test / Adequação das formas de preparação de sementes de Dalbergia miscolobium Benth para exposição ao teste de tetrazolium

2017 ◽  
Vol 1 (1) ◽  
Author(s):  
Juliana Martins de Mesquita Matos ◽  
Rosana De Carvalho Cristo Martins ◽  
Valéria Regina Bellotto ◽  
Lilian Gomes da Silva Rocha ◽  
Eloiza Aparecida Barbosa ◽  
...  
Keyword(s):  
Endangered Species ◽  
Filter Paper ◽  
Federal District ◽  
Complete Removal ◽  
Sensu Stricto ◽  
The Other ◽  
Distrito Federal ◽  
Viable Seeds ◽  
Range Test ◽  
Tetrazolium Test

Dalbergia miscolobium or Jacarandá do Cerrado is a species of legume in the Fabaceae family. It occurs in the sensu stricto Cerrado and in the dystrophic cerradão. It shows potential for landscaping and for recovering damaged areas. It is an endangered species and therefore is protected by the law that prevents cut in areas of the Federal District (Decree No. 14.783/93). The purpose of this study was to determine the best procedure to prepare seeds of Dalbergia miscolobium to assess viability in the tetrazolium test. We carried out the following treatments: i) hydration on filter paper at 25 ° C, ii) hydration on filter paper at 25 ° C followed by a cut in the tegument and iii) hydration on filter paper at 25 ° C followed bya complete removal of the tegument. The results were analyzed using analysis of variance and the Tukey range test. The analyzes showed that the best procedure to prepare seeds of Dalbergia miscolobium is the treatment in which there is a hydration followed by the complete removal of the integument. Where 78% of the seeds showed uniform staining, indicating that the seeds analyzed are of good quality. The other treatments, hydration and hydration followed by cutting, showed respectively 35% and 41% of viable seeds. RESUMO A Dalbergia miscolobium ou Jacarandá do Cerrado é uma espécie de leguminosa da família Fabaceae. Ocorre no sentido stricto Cerrado e no cerradão distrófico. Possui potencial para paisagismo e para recuperar áreas degradadas. É uma espécie ameaçada de extinção e, portanto, está protegida pela lei que previne o corte em áreas do Distrito Federal (Decreto 14.783 / 93). O objetivo deste estudo foi determinar o melhor procedimento de prepararação das sementes de Dalbergia miscolobium para serem submetidas à análise de viabilidade pelo teste de tetrazólio. Foram realizados os seguintes tratamentos: i) hidratação em papel de filtro a 25 ° C, ii) hidratação em papel de filtro a 25 ° C seguida de um corte no tegumento e iii) hidratação em papel de filtro a 25 ° C seguido de remoção completa do tegumento. Os resultados foram analisados utilizando-se a análise de variância e o teste de médias de Tukey. As análises mostraram que o melhor procedimento para preparar sementes de Dalbergia miscolobium é o tratamento em que há uma hidratação seguida pela remoção completa do tegumento, onde 78% das sementes apresentaram coloração uniforme, indicando que as sementes analisadas são de boa qualidade. Os demais tratamentos, hidratação e hidratação seguida de corte, mostraram respectivamente 35% e 41% de sementes viáveis.

EFFECTIVENESS OF ANHYDROUS AMMONIA AS A PRESERVATIVE FOR HIGH-MOISTURE ALFALFA HAY IN LARGE ROUND BALES

1986 ◽  
Vol 66 (3) ◽  
pp. 743-753 ◽  
Author(s):  
A. S. ATWAL ◽  
L. C. HESLOP ◽  
K. LIEVERS
Keyword(s):  
Ambient Temperature ◽  
Insoluble Fraction ◽  
Reference Points ◽  
Control Treatment ◽  
High Moisture ◽  
Total N ◽  
Heat Damage ◽  
Anhydrous Ammonia ◽  
Alfalfa Hay ◽  
Large Round

Two experiments were conducted in sequence to determine the effectiveness of anhydrous ammonia (AA) as a preservative of alfalfa hay stored as large round bales (LRB). In the the first experiment application of 3% AA (wt/wt) to alfalfa hay baled at about 23% moisture-content (MC) increased the temperature markedly above the control treatment and resulted in a significantly (P < 0.05) higher acid-detergent-insoluble nitrogen and acid-detergent lignin. In the second experiment application of about 1% AA slowly (over 48 h) to LRB of alfalfa hay packaged at about 30% and > 35% MC, significantly (P < 0.05) reduced the cumulative degree-days above all reference points from 35 to 65 °C. Ammoniation completely eliminated dry matter losses in storage which were highest (8.3%) for 33% MC control hay. High moisture control hay (33% MC) suffered severe heat damage with 33% of total N being in the acid-detergent-insoluble fraction as compared to less than 10% for medium-moisture (26% MC) control and even lower values for ammoniated hay. Digestibility of crude protein and energy was significantly (P < 0.05) improved by ammoniation of medium-moisture (about 30% in-to-storage) alfalfa hay. When the ammonia-treated hay was uncovered in spring, coarsely ground and stored in a hay wagon the high-moisture-ammoniated hay (> 35% MC) became moldy within 4 wk, even when the ambient temperature was about 6 °C, whereas medium-moisture-ammoniated hay (about 30% MC) showed some deterioration after 7 wk as the ambient temperature increased to about 12 °C. Key words: Ammonia, alfalfa hay, digestibility, heat damage, high-moisture hay, large bales

scholarly journals Adjustment of the methodology of the tetrazolium test for estimating viability of Eugenia uniflora L. seeds during storage

2014 ◽  
Vol 36 (3) ◽  
pp. 344-351 ◽  
Author(s):  
Daiana Karoline Kaiser ◽  
Laura Cristiane Nascimento de Freitas ◽  
Rubia Priscila Biron ◽  
Simone Cristina Simonato ◽  
Michele Fernanda Bortolini
Keyword(s):  
Seed Viability ◽  
Germination Test ◽  
Preparation Methods ◽  
Eugenia Uniflora ◽  
Paper Towel ◽  
Plastic Bags ◽  
Longitudinal Cutting ◽  
Direct Immersion ◽  
Tetrazolium Test

The study aimed to adjust the methodology of the tetrazolium test to estimate seed viability of Eugenia uniflora L. (Surinam Cherry). Seeds were collected in September 2012 and divided into four lots: freshly harvested (Lot I); stored in plastic bags in a refrigerator at 10 °C for: 15 days (Lot II); 30 days (Lot III); and 45 days (Lot IV). The freshly harvested seeds were preconditioned with direct immersion in water and wet paper towel, followed or not by longitudinal cutting. The seeds were immersed in a 0.1% tetrazolium solution for 4 hours at 30 °C. Appropriate soaking and preparation methods were applied to the seeds lots using three tetrazolium concentrations: 0.1; 0.5; and 1.0%; and four preconditioning periods (2, 4, 6 and 8 hours) at 30 °C. The viability results obtained by the tetrazolium test were compared with those of the germination test. Direct immersion of seeds in water for 24 hours at 25 °C, followed by a longitudinal cut was efficient for preconditioning the seeds. Seed staining with tetrazolium solution at a concentration of 0.5% for 2 hours at 30 °C can be used to estimate the viability of freshly harvested and stored Surinam Cherry seeds.

scholarly journals Adaptation of the tetrazolium test method for estimating the viability of sorghum seeds

2014 ◽  
Vol 36 (2) ◽  
pp. 246-252 ◽  
Author(s):  
Tereza Cristina de Carvalho ◽  
Camila Ribeiro de Souza Grzybowski ◽  
Osvaldo de Castro Ohlson ◽  
Maristela Panobianco
Keyword(s):  
Quality Control ◽  
Filter Paper ◽  
Seed Quality ◽  
Seed Viability ◽  
Test Method ◽  
Accurate Assessment ◽  
Efficient Procedure ◽  
Paper Towels ◽  
Direct Immersion ◽  
Tetrazolium Test

The tetrazolium test is part of internal programs of seed quality control because it allows a quick, reliable and accurate assessment of seed viability. The purpose of this study was to determine, among the various methodologies suggested in the literature, a practical and efficient procedure for carrying out the tetrazolium test in sorghum seeds, with a view toward a rapid estimate of their viability. Four seed lots of the simple hybrid Buster were used, testing two forms of seed pre-conditioning (direct immersion in water and between moistened paper towels); two types of preparation (longitudinal cut of the seed through the embryo with immersion of one of the halves in the tetrazolium solution or placement of the two halves on filter paper moistened with tetrazolium solution); two forms of staining (on paper and with direct immersion in the solution) and three concentrations of tetrazolium solution (0.1%, 0.5% and 1.0%). The tetrazolium test may be efficiently conducted for sorghum seeds through pre-conditioning between paper towels for 18 hours at 20 ºC, with immersion staining of one half of the seed in tetrazolium solution at 0.1% for three hours at 40 ºC.

scholarly journals Non-Deep Physiological Dormancy in Seed and Germination Requirements of Lysimachia coreana Nakai

Horticulturae ◽  
2021 ◽  
Vol 7 (11) ◽  
pp. 490
Author(s):  
Saeng Geul Baek ◽  
Jin Hyun Im ◽  
Myeong Ja Kwak ◽  
Cho Hee Park ◽  
Mi Hyun Lee ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seed Dormancy ◽  
Germination Rate ◽  
Seed Viability ◽  
Cold Stratification ◽  
Dormancy Breaking ◽  
Viability Test ◽  
Physiological Dormancy ◽  
Different Temperatures ◽  
Germination Requirements

This study aimed to determine the type of seed dormancy and to identify a suitable method of dormancy-breaking for an efficient seed viability test of Lysimachia coreana Nakai. To confirm the effect of gibberellic acid (GA3) on seed germination at different temperatures, germination tests were conducted at 5, 15, 20, 25, 20/10, and 25/15 °C (12/12 h, light/dark), using 1% agar with 100, 250, and 500 mg·L−1 GA3. Seeds were also stratified at 5 and 25/15 °C for 6 and 9 weeks, respectively, and then germinated at the same temperature. Seeds treated with GA3 demonstrated an increased germination rate (GR) at all temperatures except 5 °C. The highest GR was 82.0% at 25/15 °C and 250 mg·L−1 GA3 (4.8 times higher than the control (14.0%)). Additionally, GR increased after cold stratification, whereas seeds did not germinate after warm stratification at all temperatures. After cold stratification, the highest GR was 56.0% at 25/15 °C, which was lower than the GR observed after GA3 treatment. We hypothesized that L. coreana seeds have a non-deep physiological dormancy and concluded that 250 mg·L−1 GA3 treatment is more effective than cold stratification (9 weeks) for L. coreana seed-dormancy-breaking.

scholarly journals Flowering, Fecundity, Seed Germination, and Seed Viability of Viburnum opulus L. Cultivars

2009 ◽  
Vol 27 (1) ◽  
pp. 31-36
Author(s):  
Janine R. Conklin ◽  
James C. Sellmer
Keyword(s):  
Seed Germination ◽  
Seed Production ◽  
Seed Viability ◽  
Biological Traits ◽  
Limited Information ◽  
Viable Seed ◽  
Invasive Potential ◽  
Short Term ◽  
Viburnum Opulus ◽  
Tetrazolium Test

Abstract Mature specimens of Viburnum opulus and cultivars ‘Leonard's Dwarf’ and ‘Roseum’ were assessed over 2 years for flower and seed production, seed germination, and seed viability as determined by a tetrazolium test to understand their invasive potential. ‘Aureum’, ‘Compactum’, ‘Losely's Compact’, ‘Nanum’, and ‘Xanthocarpum’ were also tested for germination and viability of seeds. Cultivars differed in flower and seed production, seed germination, and seed viability. ‘Roseum’ prolifically produced highly viable seed that germinated at moderate rates under greenhouse conditions (8,354, 100%, and 73%, respectively). Viburnum opulus and ‘Leonard's Dwarf’ produced fewer viable seed which showed moderate to low germination rates (609, 100%, and 53%; 712, 100%, and 5%, respectively). ‘Aureum’ and ‘Xanthocarpum’ seeds germinated at moderate rates (55 and 25%, respectively) and were highly viable (100%). ‘Compactum’, ‘Losely's Compact’, and ‘Nanum’ germinated at low rates or failed to germinate (0, 0, and 5%, respectively), yet seeds were moderately viable (37, 65, and 55%, respectively). Seeds of all cultivars germinated at low rates or failed to germinate at both outdoor sites (0 to 5%) which suggests these plants may be weakly invasive. Short-term studies on biological traits such as these provide only limited information to assess the invasive potential of cultivars.

scholarly journals Seed viability of common ragweed (Ambrosia artemisiifolia L.) is affected by seed origin and age, but also by testing method and laboratory

NeoBiota ◽  
2021 ◽  
Vol 70 ◽  
pp. 193-221
Author(s):  
Rea Maria Hall ◽  
Bernhard Urban ◽  
Hana Skalova ◽  
Lenka Moravcová ◽  
Ulrike Sölter ◽  
...  
Keyword(s):  
Seed Viability ◽  
Ambrosia Artemisiifolia ◽  
Testing Methods ◽  
Triphenyltetrazolium Chloride ◽  
Viability Test ◽  
Common Ragweed ◽  
Testing Procedures ◽  
Testing Method ◽  
Seed Origin ◽  
Set Up

Common ragweed (Ambrosia artemisiifolia L.) is an annual Asteraceae species native to North America which is highly invasive across Europe and has harmful impacts, especially on human health and agriculture. Besides its wide ecological range, particularly its high reproductive power by seeds is promoting its spread to various habitats and regions. To prevent further spread and to control the plant, the European Commission funded projects and COST-Actions involving scientists from all over Europe. A joint trial was set up comprising eight different laboratories from Europe to study seed viability variation in different seed samples. Three different testing methods (viability test with 2,3,5-triphenyltetrazolium chloride (TTC), a germination test combined with a subsequent TTC test and a crush test) were tested within the EU-COST-Action SMARTER network to four different seed origins. The viability test results from different laboratories were compared for variation amongst tests and laboratories. The main aim was to optimise the reliability of testing procedures, but results revealed not only significant effects of seed origin and seed age on seed viability, but also considerable differences between the output of the individual testing methods and furthermore between laboratories. Due to these significant differences in the results of the testing labs, additionally a second test was set up. Twelve Austrian ragweed populations were used for TTC testing to obtain a precise adjustment of the testing method as well as a tight guideline for interpreting the results, particularly for the TTC state “intermediate” since a proper classification of TTC-intermediate coloured seeds is still a challenge when determining viability rates.

scholarly journals Short Communication: Tetrazolium test for evaluating viability of Capsicum annum seeds

2018 ◽  
Vol 10 (3) ◽  
pp. 142-145
Author(s):  
ADITYA KUSUMAWARDANA ◽  
BAMBANG PUJIASMANTO ◽  
PARDONO PARDONO
Keyword(s):  
Plant Growth ◽  
Laboratory Test ◽  
Seed Quality ◽  
Seed Viability ◽  
Test Results ◽  
Capsicum Annum ◽  
Laboratory Test Results ◽  
Tetrazolium Staining ◽  
Growth Vigor ◽  
Tetrazolium Test

Kusumawardana A, Pujiasmanto B, Pardono. 2018. Tetrazolium test for evaluating viability of Capsicum annum seeds. Nusantara Bioscience 10: 142-145. Seed quality is important in seeds production. This research was conducted to obtain information of topographical tetrazolium staining pattern of pepper (Capsicum annum) seeds. Tetrazolium test was conducted to determine the seed viability and plant growth vigor. Laboratory test for standard germination and field performances were performed on four lots (A, B, C, D) of pepper seeds. The viability categories pattern were determined by Root Mean Square (RMS), regression, and correlation analyses. Nine topographical patterns were recognized. The laboratory test results and field performances were compared with the topographical pattern. Combination of patterns 1,2 (embryonic axis and cotyledon completely stained) selected as viable category as it gave the least RMS value, the highest determination (R²) and correlation (r) coefficient with standard germination (RMS = 4, 06; R² = 0,761; r = 0,872). Combination of patterns 1,2 also gave the highest determination (R²) and correlation (r) coefficient with field stand (R² = 0,921; r = 0,959). The combination of patterns 1,2 is recommended for estimating plant growth performance in the field.

Sign in / Sign up

Export Citation Format

Share Document