scholarly journals Longitudinal clinical and serological survey of abdominal angiostrongyliasis in Guaporé, southern Brazil, from 1995 to 1999

2005 ◽  
Vol 38 (4) ◽  
pp. 310-315 ◽  
Author(s):  
Carlos Graeff-Teixeira ◽  
Aline Hamilton Goulart ◽  
Charles de Ornellas Brum ◽  
Antonio Carlo Laitano ◽  
Charlotte Sievers-Tostes ◽  
...  
Keyword(s):  
Southern Brazil ◽  
Zoonotic Infection ◽  
Serum Samples ◽  
Igg Antibody ◽  
Histopathologic Diagnosis ◽  
Third Stage ◽  
History Of ◽  
Parasitic Burden ◽  
One Year ◽  
Frequent Infection

Abdominal angiostrongyliasis is a zoonotic infection caused by Angiostrongylus costaricensis, a nematode with an intra-vascular location in the mesentery. Our objective was to address several aspects of the natural history of this parasitosis, in a longitudinal clinical and seroepidemiological study. A total of 179 individuals living in a rural area with active transmission in southern Brazil were followed for five years (1995-1999) resulting in yearly prevalence of 28.2%, 4.2%, 10%, 20.2% and 2.8% and incidences of 0%, 5.9%, 8% and 1.5%, respectively. Both men and woman were affected with higher frequencies at age 30-49 years. In 32 individuals serum samples were collected at all time points and IgG antibody reactivity detected by ELISA was variable and usually persisting not longer than one year. Some individual antibody patterns were suggestive of re-infection. There was no association with occurrence of abdominal pain or of other enteroparasites and there was no individual with a confirmed (histopathologic) diagnosis. Mollusks were found with infective third-stage larvae in some houses with an overall prevalence of 16% and a low parasitic burden. In conclusion, abdominal angiostrongyliasis in southern Brazil may be a frequent infection with low morbidity and a gradually decreasing serological reactivity.

scholarly journals Seroepidemiology of Chlamydia Pneumoniae in Northern Greece

2009 ◽  
Vol 7 (3) ◽  
pp. 139-144
Author(s):  
D. Chatzidimitriou ◽  
M. Exidari ◽  
G. Gioula ◽  
P. Papakonstantinou ◽  
A. Melidou ◽  
...  
Keyword(s):  
Chlamydia Pneumoniae ◽  
The Elderly ◽  
Healthy Population ◽  
Serum Samples ◽  
Igg Antibody ◽  
Northern Greece ◽  
Specific Antibodies ◽  
High Prevalence ◽  
Iga Antibodies ◽  
One Year

The prevalence of IgG and IgA antibodies to Chlamydia pneumoniae was evaluated in a group of an apparently healthy population in northern Greece. Serum samples were obtained over a period of one year (June 2006 to May 2007) from 530 individuals (300 males and 230 females, aged from 1 month to 90 years). The sera were tested for specific antibodies to C. pneumoniae by two commercial methods, an ELISA and a micro-IF assay based on the principles of MIF. The prevalence of IgG and IgA antibodies to C. pneumoniae was 53.2% and 45.9%, respectively, and was found to be unrelated to gender, even in the elderly >61 years old. The IgG antibody prevalence was low in children under 5 years old (7.7%), sharply increased by the age of 20 (40%) and continued to increase, gradually, to reach 80.1% in the elderly. IgA antibodies also increased with similar kinetics to IgG, although at a lower level (3.8–66.1%). Our results show that infection with C. pneumoniae is common in northern Greece. The high prevalence of IgA specific antibodies reported in the present study is due to primary infection at a young age, while in the elderly is probably due to infection or reinfection, although the option of persistence cannot be excluded.

Prevalence and Haemolytic Significance of Red Cell Antibodies among Dangerous Universal Donors in a Tertiary Care Hospital in South India

2019 ◽  
Author(s):  
R Amita ◽  
K Vijayalakshmi
Keyword(s):  
Standard Procedure ◽  
Tertiary Care ◽  
Care Hospital ◽  
Serum Samples ◽  
Igg Antibody ◽  
Voluntary Donor ◽  
Antibody Titres ◽  
Donor Population ◽  
History Of ◽  
Study Population

Background and Aims: A subgroup of group O individuals called ‘dangerous universal donors’ have immune (IgG) anti A and anti B antibodies which are active at 37˚C and capable of reacting with the red cells and causing lysis. The aim of this study was to find the prevalence of dangerous O group among the voluntary donor population and to assess the relation between the degree of haemolysis and the antibody titre. Materials and Methods: Group O donors excepting those with history of transfusion or pregnancy were included in the study. The serum samples were tested for haemolysins as per standard procedure. The degree of haemolysis was graded and strongly haemolytic samples were further characterised for the type of immunoglobulin class after treatment with dithiothretiol. The results were coded and analysed using SPSS software. Results: The age of the donors in this study ranged from 18 to 56 years. Majority were males. The prevalence of dangerous O group in our study population was found to be 10.75%. Within the dangerous O group samples, the titre of anti B IgG antibody was found to be higher than anti A IgG antibody. Titres for both anti A and anti B IgG antibodies ranged from 1:2 to 1:64. Conclusions: A simple screening for donor haemolysins will help in identification of strongly haemolytic samples, which are likely to have high titres of IgG, particularly anti A antibody. This will prevent transfusion of blood containing high titres of immune anti A and anti B antibodies to non O group recipients.

scholarly journals Seroepidemiological Study of Toxocariasis in Children Aged 6-14 Year Old in Sanandaj, Western Iran

2020 ◽  
Author(s):  
Yahya MAROUFI ◽  
Ashkan FARIDI ◽  
Mohammadbagher KHADEMERFAN ◽  
Fares BAHRAMI ◽  
Ghasem ZAMINI
Keyword(s):  
Demographic Data ◽  
Clinical Signs ◽  
Cross Sectional Study ◽  
Larva Migrans ◽  
Serum Samples ◽  
Igg Antibody ◽  
Cross Sectional ◽  
Medical Diagnostic ◽  
History Of ◽  
Low Prevalence

Background: Toxocariasis is a disease caused by Toxocara nematodes and occurs from consuming their eggs. The main hosts of these worms are dogs and cats. The disease in humans becomes a visceral larva migrans (VLM). This descriptive cross-sectional study was conducted to determine the prevalence of toxocariasis in children aged 6–14 years. Methods: This cross-sectional descriptive study was conducted from Jun 1 2016 to Dec 1 2017 in Sanandaj, west of Iran. A total of 182 serum samples were collected from children age 6 to14 yr referred to medical diagnostic laboratories. Demographic data (age, sex, and parents' literacy status), clinical signs (cough, headache, fever, abdominal pain), and the history of contact with dogs and cats was collected by a questionnaire. The presence of anti-Toxocara IgG antibody was detected by T. canis IgG ELISA (IBL, Germany) kit. Results: Of 182 subjects, 97 (53.3%) were male and 85 (46.7%) female. The average age was 9.2 years. Antibodies against T. canis were positive in three cases (1.65%) of all the studied subjects. Conclusions: The results showed a low prevalence of toxocariasis in children studied.

scholarly journals Quantitative SARS-CoV-2 Antibody Screening of Healthcare Workers in the Southern Part of Kyoto City During the COVID-19 Pre-pandemic Period

2020 ◽  
Vol 8 ◽  
Author(s):  
Kohei Fujita ◽  
Shinpei Kada ◽  
Osamu Kanai ◽  
Hiroaki Hata ◽  
Takao Odagaki ◽  
...  
Keyword(s):  
Healthcare Workers ◽  
Early Stage ◽  
Case Fatality ◽  
Serum Samples ◽  
Igg Antibody ◽  
Kyoto City ◽  
History Of ◽  
Heavy Burden ◽  
Antibody Levels ◽  
Mental And Physical Health

Background: The coronavirus disease-2019 (COVID-19) pandemic is associated with a heavy burden on the mental and physical health of patients, regional healthcare resources, and global economic activity. While understanding of the incidence and case-fatality rates has increased, there are limited data concerning seroprevalence of antibodies against the severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) in healthcare workers during the pre-pandemic period. This study aimed to quantitatively evaluate seroprevalence of SARS-CoV-2 antibodies in healthcare workers in the southern part of Kyoto city, Japan.Methods: We prospectively recruited healthcare workers from a single hospital between April 10 and April 20, 2020. We collected serum samples from these participants and quantitatively evaluated SARS-CoV-2 IgG antibody levels using enzyme-linked immunosorbent assays.Results: Five (5.4%), 15 (16.3%), and 72 (78.3%) participants showed positive, borderline, and negative serum SARS-CoV-2 IgG antibody status, respectively. We found the mean titer associated with each antibody status (overall, positive, borderline, and negative) was clearly differentiated. Participants working at the otolaryngology department and/or with a history of seasonal common cold symptoms had a significantly higher SARS-CoV-2 IgG antibody titer (p = 0.046, p = 0.046, respectively).Conclusions: Five (5.4%) and 15 (16.3%) participants tested positive and borderline, respectively, for SARS-CoV-2 IgG antibody during the COVID-19 pre-pandemic period. These rates were higher than expected, based on government situation reports. These findings suggest that COVID-19 had already spread within the southern part of Kyoto city at the early stage of the pandemic.

scholarly journals Serological Diagnosis of Brucella Infections in Odontocetes

2009 ◽  
Vol 16 (6) ◽  
pp. 906-915 ◽  
Author(s):  
Gabriela Hernández-Mora ◽  
Charles A. Manire ◽  
Rocío González-Barrientos ◽  
Elías Barquero-Calvo ◽  
Caterina Guzmán-Verri ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
Brucella Melitensis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Dot Blot ◽  
Serum Samples ◽  
Igg Antibody ◽  
Serological Tests ◽  
Diagnostic Potential ◽  
Control Serum ◽  
History Of

ABSTRACT Brucella ceti causes disease in Odontoceti. The absence of control serum collections and the diversity of cetaceans have hampered the standardization of serological tests for the diagnosis of cetacean brucellosis. Without a “gold” standard for sensitivity and specificity determination, an alternative approach was followed. We designed an indirect enzyme-linked immunosorbent assay (iELISA) that recognizes immunoglobulins G (IgGs) from 17 odontocete species as a single group. For the standardization, we used Brucella melitensis and Brucella abortus lipopolysaccharides, serum samples from seven resident odontocetes with no history of infectious disease displaying negative rose bengal test (RBT) reactions, and serum samples from seven dolphins infected with B. ceti. We compared the performance of the iELISA with those of the protein G ELISA (gELISA), the competitive ELISA (cELISA), and the immunofluorescence (IF) and dot blot (DB) tests, using 179 odontocete serum samples and RBT as the reference. The diagnostic potential based on sensitivity and specificity of the iELISA was superior to that of gELISA and cELISA. The correlation and agreement between the iELISA and the gELISA were relatively good (R i/g 2 = 0.65 and κi/g = 0.66, respectively), while the correlation and agreement of these two ELISAs with cELISA were low (R i/c 2 = 0.46, R g/c 2 = 0.37 and κi/c = 0.62, κg/c = 0.42). In spite of using the same anti-odontocete IgG antibody, the iELISA was more specific than were the IF and DB tests. An association between high antibody titers and the presence of neurological symptoms in dolphins was observed. The prediction is that iELISA based on broadly cross-reacting anti-dolphin IgG antibody would be a reliable test for the diagnosis of brucellosis in odontocetes, including families not covered in this study.

scholarly journals Quantitative SARS-CoV-2 antibody screening of healthcare workers in the southern part of Kyoto city during the COVID-19 peri-pandemic period

2020 ◽  
Author(s):  
Kohei Fujita ◽  
Shinpei Kada ◽  
Osamu Kanai ◽  
Hiroaki Hata ◽  
Takao Odagaki ◽  
...  
Keyword(s):  
Healthcare Workers ◽  
Early Stage ◽  
Case Fatality ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Igg Antibody ◽  
Kyoto City ◽  
History Of ◽  
Heavy Burden ◽  
Antibody Levels

Background: The coronavirus disease-2019 (COVID-19) pandemic is associated with a heavy burden on the mental and physical health of patients, regional healthcare resources, and global economic activity. While our understanding of the incidence and case-fatality rates increases, data on seroprevalence of antibodies against the severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) in healthcare workers during the peri-pandemic period is insufficient. This study quantitatively evaluated seroprevalence of SARS-CoV-2 antibody in healthcare workers in the southern part of Kyoto city, Japan. Methods: We prospectively recruited healthcare workers from a single hospital between April 10 and April 20, 2020. We collected serum samples from these participants and quantitatively evaluated SARS-CoV-2 IgG antibody levels by enzyme-linked immunosorbent assay. Results: Five (5.4%), 15 (16.3%), and 72 (78.3%) participants showed positive, borderline, and negative serum SARS-CoV-2 IgG antibody status, respectively. We found the mean titer associated with each antibody status (overall, positive, borderline, and negative) was clearly differentiated. Participants working at the otolaryngology department and/or having a history of seasonal common cold symptoms had a significantly higher titer of SARS-CoV-2 IgG antibody (p=0.046, p=0.046, respectively). Conclusions: Five (5.4%) and 15 (16.3%) participants tested positive and borderline, respectively, for SARS-CoV-2 IgG antibody during the COVID-19 peri-pandemic period. These rates were higher than expected based on government situation reports. The present findings suggest that COVID-19 was already spread in the southern part of Kyoto city at the early stage of pandemic.

scholarly journals The Immunoglobulin (IgG) Antibody Response to OspA and OspB Correlates with Severe and Prolonged Lyme Arthritis and the IgG Response to P35 Correlates with Mild and Brief Arthritis

1999 ◽  
Vol 67 (1) ◽  
pp. 173-181 ◽  
Author(s):  
Evren Akin ◽  
Gail L. McHugh ◽  
Richard A. Flavell ◽  
Erol Fikrig ◽  
Allen C. Steere
Keyword(s):  
Antibody Response ◽  
Protein C ◽  
Surface Protein ◽  
Early Arthritis ◽  
Early Infection ◽  
Lyme Arthritis ◽  
Serum Samples ◽  
Igg Antibody ◽  
History Of ◽  
Serial Serum

ABSTRACT In an effort to implicate immune responses to specificBorrelia burgdorferi proteins that may have a role in chronic Lyme arthritis, we studied the natural history of the antibody response to B. burgdorferi in serial serum samples from 25 patients monitored throughout the course of Lyme disease. In these patients, the immunoglobulin G (IgM) and IgG antibody responses to 10 recombinant B. burgdorferi proteins, determined during early infection, early arthritis, and maximal arthritis, were correlated with the severity and duration of maximal arthritis. The earliest responses were usually to outer surface protein C (OspC), P35, P37, and P41; reactivity with OspE, OspF, P39, and P93 often developed weeks later; and months to years later, 64% of patients had responses to OspA and OspB. During early infection and early arthritis, the levels of IgG antibody to P35 correlated inversely with the subsequent severity or duration of maximal arthritis. In contrast, during periods of maximal arthritis, the levels of IgG antibody to OspA and OspB, especially to a C-terminal epitope of OspA, correlated directly with the severity and duration of arthritis. Thus, the higher the IgG antibody response to P35 earlier in the infection, the milder and briefer the subsequent arthritis, whereas during maximal arthritis, the higher the IgG response to OspA and OspB, the more severe and prolonged the arthritis.

scholarly journals Latent and AsymptomaticToxocaraInfection among Young Population in Northwest Iran: The Necessity of Informing People as a Potential Health Risk

Scientifica ◽  
2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Tina Momeni ◽  
Mahmoud Mahami-Oskouei ◽  
Esmaeil Fallah ◽  
Abdolrasoul Safaiyan ◽  
Leyla Mahami-Oskouei
Keyword(s):  
Public Health Problem ◽  
Serum Samples ◽  
Igg Antibody ◽  
Chi Square ◽  
Potential Health ◽  
Antibody Assays ◽  
History Of ◽  
Northwest Iran ◽  
And Gender ◽  
Chi Square Analysis

Objectives. This study was designed to determine the frequency of anti-Toxocaraantibodies in youngsters aging from 2 to 20 years in northwest Iran.Materials and Methods. 397 samples were taken randomly almost equally from four locations in Urmia, west Azerbaijan, during August 2014 to September 2015. Anti-ToxocaraIgG antibody assays were done on sera by using ELISA kit (IBL, Germany). In order to prevent cross-reaction, the samples of the patients who are infected with other parasites in stool exam, especially Ascarididae family, were also excluded. SPSS 16.0 software was used for statistical analysis.Results. 12 (3%) of the serum samples were positive for anti-ToxocaraIgG. According to the Chi-square analysis, risk factors such as mother’s educational level, keeping dogs or cats as pets, and history of coughing were related toToxocarainfection (P<0.05). There was no relationship between toxocariasis and gender, history of onychophagy, pica, fever, abdominal pain, and anorexia; however, we found a significant relationship betweenToxocarainfection and chronic coughing (P=0.045).Conclusion. Toxocariasis in northwest Iran can be considered as a public health problem. This study may also help to increase the awareness about this infection.

scholarly journals Covert cases of Severe Acute Respiratory Syndrome Coronavirus 2: An obscure but present danger in regions endemic for Dengue and Chikungunya viruses

PLoS ONE ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. e0244937
Author(s):  
Lorenzzo Lyrio Stringari ◽  
Michel Norbim de Souza ◽  
Nésio Fernandes de Medeiros Junior ◽  
Jaqueline Pegoretti Goulart ◽  
Camila Giuberti ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Clinical Symptoms ◽  
Previous History ◽  
Cross Reactivity ◽  
Serum Samples ◽  
Igg Antibody ◽  
Acute Infections ◽  
History Of ◽  
Chemiluminescent Microparticle Immunoassay ◽  
The Impact

Background The impact of SARS-CoV-2 in regions endemic for both Dengue and Chikungunya is still not fully understood. Considering that symptoms/clinical features displayed during Dengue, Chikungunya and SARS-CoV-2 acute infections are similar, undiagnosed cases of SARS-CoV-2 in co-endemic areas may be more prevalent than expected. This study was conducted to assess the prevalence of covert cases of SARS-CoV-2 among samples from patients with clinical symptoms compatible with either Dengue or Chikungunya viral infection in the state of Espírito Santo, Brazil. Methods Presence of immunoglobulin G (IgG) antibody specific to SARS-CoV-2 nucleoprotein was detected using a chemiluminescent microparticle immunoassay in samples from 7,370 patients, without previous history of COVID-19 diagnosis, suspected of having either Dengue (n = 1,700) or Chikungunya (n = 7,349) from December 1st, 2019 to June 30th, 2020. Findings Covert cases of SARS-CoV-2 were detected in 210 (2.85%) out of the 7,370 serum samples tested. The earliest undiagnosed missed case of COVID-19 dated back to a sample collected on December 18, 2019, also positive for Dengue Virus. Cross-reactivity with either Dengue virus or other common coronaviruses were not observed. Interpretation Our findings demonstrate that concomitant Dengue or Chikungunya outbreaks may difficult the diagnosis of SARS-CoV-2 infections. To our knowledge, this is the first study to demonstrate, with a robust sample size (n = 7,370) and using highly specific and sensitive chemiluminescent microparticle immunoassay method, that covert SARS-CoV-2 infections are more frequent than previously expected in Dengue and Chikungunya hyperendemic regions. Moreover, our results suggest that SAR-CoV-2 cases were occurring prior to February, 2020, and that these undiagnosed missed cases may have contributed to the fast expansion of SARS-CoV-2 outbreak in Brazil. Data presented here demonstrate that in arboviral endemic regions, SARS-CoV-2 infection must be always considered, regardless of the existence of a previous positive diagnosis for Dengue or Chikungunya.

scholarly journals A Survey of Vaccine-Induced Measles IgG Antibody Titer to Verify Temporal Changes in Response to Measles Vaccination in Young Adults

Vaccines ◽  
2019 ◽  
Vol 7 (3) ◽  
pp. 118
Author(s):  
Hiraku Sasaki ◽  
Tomoko Fukunaga ◽  
Ai Asano ◽  
Yoshio Suzuki ◽  
Yuko Nakanishi ◽  
...  
Keyword(s):  
Young Adults ◽  
Measles Virus ◽  
Serum Samples ◽  
Igg Antibody ◽  
Chiba Prefecture ◽  
History Of ◽  
Antibody Titers ◽  
Multivariable Regression ◽  
Measles Vaccination ◽  
Specific Igg

In Japan, sporadic measles cases increased rapidly in 2019 compared to the past six years. To clarify the persistence of immunity against measles in young adults, this study explored the persistence of immunoglobulin G (IgG) antibody titers against the measles virus in 17- to 24-year-old young participants who reside in the Chiba prefecture of Japan. Measles-specific IgG antibody titers, determined by enzyme immunoassay in serum samples collected from 506 participants, were assessed through statistical analyses. Multivariable regression analysis revealed that the distribution of measles IgG antibody titers was significantly correlated with a medical history of measles (P < 0.05), while there was no significant correlation between the number of vaccinations related to measles IgG titers. Furthermore, measles IgG titers tended to decrease, as revealed by the temporal change in IgG titers, during the elapsed period after the last vaccination (P = 0.08). These results indicate that periodic vaccination against measles is required to prevent sporadic measles infection in young and older adults.

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