scholarly journals Seroepidemiology of Chlamydia Pneumoniae in Northern Greece

2009 ◽  
Vol 7 (3) ◽  
pp. 139-144
Author(s):  
D. Chatzidimitriou ◽  
M. Exidari ◽  
G. Gioula ◽  
P. Papakonstantinou ◽  
A. Melidou ◽  
...  
Keyword(s):  
Chlamydia Pneumoniae ◽  
The Elderly ◽  
Healthy Population ◽  
Serum Samples ◽  
Igg Antibody ◽  
Northern Greece ◽  
Specific Antibodies ◽  
High Prevalence ◽  
Iga Antibodies ◽  
One Year

The prevalence of IgG and IgA antibodies to Chlamydia pneumoniae was evaluated in a group of an apparently healthy population in northern Greece. Serum samples were obtained over a period of one year (June 2006 to May 2007) from 530 individuals (300 males and 230 females, aged from 1 month to 90 years). The sera were tested for specific antibodies to C. pneumoniae by two commercial methods, an ELISA and a micro-IF assay based on the principles of MIF. The prevalence of IgG and IgA antibodies to C. pneumoniae was 53.2% and 45.9%, respectively, and was found to be unrelated to gender, even in the elderly >61 years old. The IgG antibody prevalence was low in children under 5 years old (7.7%), sharply increased by the age of 20 (40%) and continued to increase, gradually, to reach 80.1% in the elderly. IgA antibodies also increased with similar kinetics to IgG, although at a lower level (3.8–66.1%). Our results show that infection with C. pneumoniae is common in northern Greece. The high prevalence of IgA specific antibodies reported in the present study is due to primary infection at a young age, while in the elderly is probably due to infection or reinfection, although the option of persistence cannot be excluded.

scholarly journals The impact of non-steroidal anti-inflammatory drugs use in elderly cardiovascular patients: an observational study from tertiary care in South India

2019 ◽  
Vol 7 (5) ◽  
pp. 1787
Author(s):  
Sadhna Sharma ◽  
Biju Govind ◽  
Kondal Rao
Keyword(s):  
Observational Study ◽  
Elderly Patients ◽  
Tertiary Care ◽  
The Elderly ◽  
Left Ventricular ◽  
Risk Of Death ◽  
Increased Risk ◽  
High Prevalence ◽  
One Year ◽  
The Impact

Background: Long-term use of NSAIDs, by patients having cardiovascular conditions, has shown to increase the risk of cardiovascular events and increased risk of death. Hence, the study was conducted to determine the complications related to NSAID use by the elderly patients with cardiovascular disease (CVD).Methods: The study was a single-center prospective observational study conducted November 2017 to October 2018. Elderly patients (>60 years) suffering from various CVDs and reported NSAID intake daily for at least one month were included. A questionnaire included demographic, treatment related history and complete details of NSAIDs intake including nature, dose, indication, source etc. The same questionnaire was again filled at the end of one-year follow-up.Results: A total of 100 participants were included in the study. The mean age was 72±8.6 years. Majority of the patients (93%) had hypertension, and 69% of the patient had previous MI.  Five NSAIDs (diclofenac, ibuprofen, mefenamic acid, naproxen, and ketorolac) were used routinely. At least one over the counter NSAID used was reported by 86%, 57% were prescribed at least one NSAIDs by their orthopaedics and physicians. At the end of 1-year follow-up, authors found that 71% had MI (2% increase), 4% developed reinfarction, 20% had severe left ventricular failure (4% increase), 7% had atrial fibrillation (1% increase), and 2% patients died and 63% patients reported raise in systolic blood pressure by 5mmHg.Conclusions: High prevalence of concomitant NSAID use among elderly CVD patients, which might be contributing towards increase in CVS morbidity and mortality.

scholarly journals Immunoglobulin G and A Antibody Responses to Bacteroides forsythus and Prevotella intermedia in Sera and Synovial Fluids of Arthritis Patients

2003 ◽  
Vol 10 (6) ◽  
pp. 1043-1050 ◽  
Author(s):  
Ketil Moen ◽  
Johan G. Brun ◽  
Tor Magne Madland ◽  
Turid Tynning ◽  
Roland Jonsson
Keyword(s):  
Immune Responses ◽  
Immunoglobulin G ◽  
Enzyme Linked Immunosorbent Assay ◽  
Prevotella Intermedia ◽  
Igg Antibodies ◽  
Serum Samples ◽  
Igg Antibody ◽  
Iga Antibodies ◽  
Iga Antibody ◽  
Antibody Levels

ABSTRACT The objective of the present study was to investigate immunoglobulin G (IgG) and IgA antibody immune responses to Porphyromonas gingivalis, Prevotella intermedia, Bacteroides forsythus, and Candida albicans in the sera of patients with rheumatoid arthritis (RA), the synovial fluid (SF) of patients with RA (RA-SF samples), and the SF of patients without RA (non-RA-SF samples). An enzyme-linked immunosorbent assay was used to determine IgG and IgA antibody levels in 116 serum samples from patients with RA, 52 RA-SF samples, and 43 non-RA-SF samples; and these were compared with those in SF samples from 9 patients with osteoarthritis (OA-SF samples) and the blood from 100 donors (the control [CTR] group). Higher levels of IgG antibodies against B. forsythus (P < 0.0001) and P. intermedia (P < 0.0001) were found in non-RA-SF samples than in OA-SF samples, and higher levels of IgG antibodies against B. forsythus (P = 0.003) and P. intermedia (P = 0.024) were found in RA-SF samples than in OA-SF samples. Significantly higher levels of IgA antibodies against B. forsythus were demonstrated in both RA-SF and non-RA-SF samples than in OA-SF samples. When corrected for total Ig levels, levels of IgG antibody against B. forsythus were elevated in RA-SF and non-RA-SF samples compared to those in OA-SF samples. Lower levels of Ig antibodies against B. forsythus were found in the sera of patients with RA than in the plasma of the CTR group for both IgG (P = 0.003) and IgA (P < 0.0001). When corrected for total Ig levels, the levels of IgG and IgA antibodies against B. forsythus were still found to be lower in the sera from patients with RA than in the plasma of the CTR group (P < 0.0001). The levels of antibodies against P. gingivalis and C. albicans in the sera and SF of RA and non-RA patients were comparable to those found in the respective controls. The levels of IgG and IgA antibodies against B. forsythus were elevated in SF from patients with RA and non-RA-SF samples compared to those in OA-SF samples. Significantly lower levels of IgG and IgA antibodies against B. forsythus were found in the sera of patients with RA than in the plasma of the CTR group. This indicates the presence of an active antibody response in synovial tissue and illustrates a potential connection between periodontal and joint diseases.

scholarly journals Prevalence and Persistence of Chlamydia pneumoniae Antibodies in Healthy Laboratory Personnel in Finland

2005 ◽  
Vol 12 (5) ◽  
pp. 654-659 ◽  
Author(s):  
Mika Paldanius ◽  
Aini Bloigu ◽  
Marianne Alho ◽  
Maija Leinonen ◽  
Pekka Saikku
Keyword(s):  
Chlamydia Pneumoniae ◽  
Study Groups ◽  
Incidence Rates ◽  
Annual Incidence ◽  
Serum Samples ◽  
Iga Antibodies ◽  
Laboratory Workers ◽  
Commercial Enzyme Immunoassay ◽  
Species Specific

ABSTRACT The rates of Chlamydia pneumoniae seroconversions suggesting acute primary infections or reinfections and the prevalences of antibodies were followed up among healthy laboratory workers. Annual serum samples were collected from 47 persons in Helsinki from 1958 to 1990 and from 40 persons in Oulu from 1994 to 1999. C. pneumoniae species-specific immunoglobulin G (IgG), IgA, and IgM antibodies were measured by microimmunofluorescence (MIF) in 407 sera from Helsinki. The 185 sera collected in Oulu were tested both by MIF and by commercial enzyme immunoassay (EIA). During the follow-up periods of 31 years in Helsinki and 6 years in Oulu, seroconversions were demonstrated by MIF in 45% and 15% of the study groups, respectively. In Helsinki 9% of the persons seroconverted twice during the follow-up period. By MIF, the total incidence rate per 100 person-years at risk was 6.9 in Helsinki and 4.9 in Oulu, and annual incidence rates varied from 0 to 15.4. By EIA, annual incidence rates in Oulu varied from 0 to 10.8. The seroconversions by MIF were usually not confirmed by EIA and vice versa. Prevalence and persistence rates, respectively, of IgA antibodies were higher in EIA (62% and 26%) than in MIF (26% and 17%), whereas the figures for IgG were quite similar. The prevalence of IgG and IgA antibodies was higher in older persons than in younger ones. The presence of antibodies did not offer protection from reinfection.

scholarly journals Longitudinal clinical and serological survey of abdominal angiostrongyliasis in Guaporé, southern Brazil, from 1995 to 1999

2005 ◽  
Vol 38 (4) ◽  
pp. 310-315 ◽  
Author(s):  
Carlos Graeff-Teixeira ◽  
Aline Hamilton Goulart ◽  
Charles de Ornellas Brum ◽  
Antonio Carlo Laitano ◽  
Charlotte Sievers-Tostes ◽  
...  
Keyword(s):  
Southern Brazil ◽  
Zoonotic Infection ◽  
Serum Samples ◽  
Igg Antibody ◽  
Histopathologic Diagnosis ◽  
Third Stage ◽  
History Of ◽  
Parasitic Burden ◽  
One Year ◽  
Frequent Infection

Abdominal angiostrongyliasis is a zoonotic infection caused by Angiostrongylus costaricensis, a nematode with an intra-vascular location in the mesentery. Our objective was to address several aspects of the natural history of this parasitosis, in a longitudinal clinical and seroepidemiological study. A total of 179 individuals living in a rural area with active transmission in southern Brazil were followed for five years (1995-1999) resulting in yearly prevalence of 28.2%, 4.2%, 10%, 20.2% and 2.8% and incidences of 0%, 5.9%, 8% and 1.5%, respectively. Both men and woman were affected with higher frequencies at age 30-49 years. In 32 individuals serum samples were collected at all time points and IgG antibody reactivity detected by ELISA was variable and usually persisting not longer than one year. Some individual antibody patterns were suggestive of re-infection. There was no association with occurrence of abdominal pain or of other enteroparasites and there was no individual with a confirmed (histopathologic) diagnosis. Mollusks were found with infective third-stage larvae in some houses with an overall prevalence of 16% and a low parasitic burden. In conclusion, abdominal angiostrongyliasis in southern Brazil may be a frequent infection with low morbidity and a gradually decreasing serological reactivity.

scholarly journals A Paper-based Immunofluorescent Device for the Detection of SARS-CoV-2 Humanized Antibody

2021 ◽  
Author(s):  
Surasak Kasetsirikul ◽  
Muhammad Umer ◽  
Narshone Soda ◽  
Kamalalayam Rajan Sreejith ◽  
Muhammad J. A. Shiddiky ◽  
...  
Keyword(s):  
Human Serum ◽  
Point Of Care ◽  
Serum Samples ◽  
Igg Antibody ◽  
Healthy Human ◽  
Specific Antibodies ◽  
Human Serum Samples ◽  
Humanized Antibody ◽  
Relative Standard ◽  
Humanized Antibodies

We report on an immunofluorescent paper-based assay for the detection of severe acute respiratory symptom coronavirus 2 (SARS-CoV-2) humanized antibody. The paper-based device was fabricated by using lamination technique for easy and optimized handling. Our approach utilises a two-step strategy that involves (i) initial coating of the paper-electrode with recombinant SARS-CoV-2 nucleocapsid antigen to capture the target SARS-CoV-2 specific antibodies, and (ii) subsequent detection of SARS-CoV-2 antibodies using fluorophore-conjugated IgG antibody. The fluorescence readout was observed with fluorescence microscopy. The images were processed and quantified using a MATLAB program. The assay can selectively detect SARS-CoV-2 humanized antibodies spiked in PBS and healthy human serum samples with the relative standard deviation of approximately 6.4% (for n = 3). It has broad dynamic ranges (1 ng to 50 ng/&micro;L in PBS and 5 to 100 ng/&micro;L in human serum samples) for SARS-CoV-2 humanized antibodies with the detection limits of 2 ng/&micro;L (0.025 IU/mL) and 10 ng/&micro;L (0.125 IU/mL) in PBS and human serum samples, respectively. We believe that our assay has the potential to be used as a simple, rapid, and inexpensive paper-based diagnostic device with a portable fluorescent reader to provide point-of-care diagnosis. This assay can be used for rapid examination of a large batch of samples toward clinical screening of SARS-CoV-2 specific antibodies as a confirmed infected active case or to evaluate the immune response to a SARS-CoV-2 vaccine.

scholarly journals Status of Immunity Against the Hepatitis A Virus in Healthy Population: A Report From Southeastern Iran

2021 ◽  
Vol 16 (4) ◽  
Author(s):  
Alireza Bakhshipour ◽  
Narjes Sargolzaie ◽  
Raheleh Rafaiee
Keyword(s):  
General Population ◽  
Rural Areas ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Published Data ◽  
Healthy Population ◽  
Serum Samples ◽  
Igg Antibody ◽  
Urban And Rural Areas

Background: Recently, epidemiological studies on hepatitis A virus (HAV) infection showed the seroprevalence has been changing due to changes in lifestyle. To the best of our knowledge, there have been no published data on the seropositivity of HAV in Zahedan, southeastern Iran. Objectives: This study aimed to investigate the seroprevalence of HAV immunoglobulin G (IgG) antibody in Zahedan, southeastern Iran, to provide the required information for better planning in preventive strategies. Methods: In this cross-sectional study, using the available sampling method, a total of 250 serum samples (18 years and above) in both the urban and rural areas of Zahedan were evaluated for anti-HAV IgG by enzyme-linked immunosorbent assay. Results: Based on the results, it was observed that 228 out of 250 (91.2%) serum samples were positive for HAV IgG antibody. Male gender, family size, parents’ education, mother’s occupation, and history of jaundice before the age of 12 years were associated with positive HAV antibody (P < 0.001). The seroprevalence HAV rates were not statistically different between the residents of urban and rural regions. Conclusions: The seropositivity of HAV is high in both the urban and rural areas of Zahedan, Iran. Therefore, the HAV vaccination of the general population is not necessary. It is recommended to monitor HAV seroprevalence in the general population to determine high-risk groups, including anti-HAV seronegative individuals, for HAV vaccination in the residents of the southeast border.

scholarly journals Testing IgG antibodies against the RBD of SARS-CoV-2 is sufficient and necessary for COVID-19 diagnosis

PLoS ONE ◽  
2020 ◽  
Vol 15 (11) ◽  
pp. e0241164 ◽  
Author(s):  
Victoria Indenbaum ◽  
Ravit Koren ◽  
Shiri Katz-Likvornik ◽  
Mayan Yitzchaki ◽  
Osnat Halpern ◽  
...  
Keyword(s):  
Igg Antibodies ◽  
Serum Samples ◽  
Igg Antibody ◽  
Past Exposure ◽  
Iga Antibodies ◽  
Global Spread ◽  
Diagnostic Capacity ◽  
Antibody Levels ◽  
Antibody Kinetics ◽  
Kinetics Of

The COVID-19 pandemic and the fast global spread of the disease resulted in unprecedented decline in world trade and travel. A critical priority is, therefore, to quickly develop serological diagnostic capacity and identify individuals with past exposure to SARS-CoV-2. In this study serum samples obtained from 309 persons infected by SARS-CoV-2 and 324 of healthy, uninfected individuals as well as serum from 7 COVID-19 patients with 4–7 samples each ranging between 1–92 days post first positive PCR were tested by an “in house” ELISA which detects IgM, IgA and IgG antibodies against the receptor binding domain (RBD) of SARS-CoV-2. Sensitivity of 47%, 80% and 88% and specificity of 100%, 98% and 98% in detection of IgM, IgA and IgG antibodies, respectively, were observed. IgG antibody levels against the RBD were demonstrated to be up regulated between 1–7 days after COVID-19 detection, earlier than both IgM and IgA antibodies. Study of the antibody kinetics of seven COVID 19 patients revealed that while IgG levels are high and maintained for at least 3 months, IgM and IgA levels decline after a 35–50 days following infection. Altogether, these results highlight the usefulness of the RBD based ELISA, which is both easy and cheap to prepare, to identify COVID-19 patients even at the acute phase. Most importantly our results demonstrate that measuring IgG levels alone is both sufficient and necessary to diagnose past exposure to SARS-CoV-2.

Prevalence of Specific Antibodies to Chlamydia pneumoniae in Korea

1998 ◽  
Vol 36 (11) ◽  
pp. 3426-3428 ◽  
Author(s):  
Tae Yeal Choi ◽  
Duck An Kim ◽  
Sin Kyung Kim ◽  
Jung Oak Kang ◽  
Sung Soo Park ◽  
...  
Keyword(s):  
Cord Blood ◽  
Respiratory Infection ◽  
Respiratory Diseases ◽  
Chlamydia Pneumoniae ◽  
Healthy Individuals ◽  
Serum Samples ◽  
Specific Antibodies ◽  
Acute Respiratory Diseases ◽  
Blood Specimens

To clarify the endemic status of Chlamydia pneumoniaein Korea, the incidence of antibodies in 564 serum samples from healthy individuals, patients with respiratory infection, and cord blood specimens was evaluated. We conclude that C. pneumoniaeinfection is highly endemic in Korea and that this infection is associated with acute respiratory diseases.

scholarly journals Detection of specific IgG, IgM, IgE and IgG subclass antibodies for serological diagnosis of human cystic echinococcosis

2015 ◽  
Vol 52 (2) ◽  
pp. 85-88 ◽  
Author(s):  
M. I. Naik ◽  
R. Kumar Tenguria ◽  
Ehtishamul Haq
Keyword(s):  
Cystic Echinococcosis ◽  
Diagnostic Sensitivity ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Parasitic Infections ◽  
Igg Subclass ◽  
Healthy Controls ◽  
Serum Samples ◽  
Igg Antibody ◽  
Specific Antibodies

SummaryAn enzyme linked immunosorbent assay (ELISA), based on sheep hydatid cyst fluid antigen was used for the detection of specific antibodies of IgG, IgM, IgE and IgG subclass in the serum samples of 62 clinically and radiologically diagnosed cystic echinococcosis (CE) patients, 8 clinically suspected cases of CE, 25 other parasitic disease controls and 25 healthy controls. The diagnostic sensitivity in the clinically and radiologically suggestive cases (n = 62) for IgG antibody detection was highest (93 %), followed by IgE, IgG4, IgG1, IgG2, IgM and IgG3 with 89 %, 87 %, 85 %, 76 %, 70 % and 55 % respectively. The detection of specific IgE, IgG1 and IgG4 antibody showed the higher diagnostic sensitivity and specificity to the extent that they can be safely used as better substitute to IgG. Even though, the diagnostic sensitivity of IgG was highest (93%) but was less specific (88 %) due to the frequent non-specific reactions in the sera of patients with other parasitic infections and healthy controls. None of the sera samples from healthy controls gave non-specific reaction with IgE, IgG1 and IgG4 and there was a considerably reduced cross-reaction with these antibodies. The most discriminatory and specific antibodies found in this study belonged to IgE, IgG1 and IgG4; therefore, these antibodies may serve as useful diagnostic markers for CE.

scholarly journals Serological survey for canine angiostrongylosis in Slovakia

2015 ◽  
Vol 52 (3) ◽  
pp. 205-210 ◽  
Author(s):  
M. Miterpáková ◽  
M. Schnyder ◽  
R. Schaper ◽  
Z. Hurníková ◽  
V. Čabanová
Keyword(s):  
Current Distribution ◽  
Dirofilaria Immitis ◽  
Parasitic Infection ◽  
Angiostrongylus Vasorum ◽  
Parasitic Disease ◽  
Serological Survey ◽  
Serum Samples ◽  
Specific Antibodies ◽  
Life Threatening ◽  
One Year

Abstract In recent years Angiostrongylus vasorum has become another important heart parasite of dogs besides Dirofilaria immitis, with intense spread into new areas of Europe. The first two cases of canine angiostrongylosis in Slovakia were observed in 2013, demonstrating that this life-threatening parasitic disease of dogs has expanded into this territory too. One year after the first A. vasorum findings, a serological survey was conducted to assess the current distribution of this parasitic infection in dogs from Slovakia. Serum samples from 225 dogs were collected from 29 veterinary practices situated in 22 districts of Slovakia and tested by ELISA for the presence of circulating A. vasorum antigens and additionally for the detection of specific antibodies against A. vasorum. Fourteen samples (6.22 %) were seropositive in at least one ELISA. Of these, 7 dogs (3.11 %) were only antibody-positive and 4 dogs (1.78 %) were positive only for circulating A. vasorum antigen. Three animals out of 225 examined (1.33 %) were positive in both ELISAs

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