scholarly journals Steroidal saponin concentrations in Brachiaria decumbens and B. brizantha at different developmental stages

2008 ◽  
Vol 39 (1) ◽  
pp. 279-281 ◽  
Author(s):  
Karine Bonucielli Brum ◽  
Mitsue Haraguchi ◽  
Mirella Biasoli Garutti ◽  
Fernanda Nogarol Nóbrega ◽  
Beneval Rosa ◽  
...  
Keyword(s):  
Thin Layer ◽  
Developmental Stages ◽  
Ethanolic Extract ◽  
Spectrophotometric Analysis ◽  
Steroidal Saponin ◽  
Steroidal Saponins ◽  
Brachiaria Decumbens ◽  
Aerial Parts ◽  
Seed Fall ◽  
Layer Chromatography

Brachiaria species contain steroidal saponins and are involved in outbreaks of hepatogenous photosensitization. This research presents the levels of a steroidal saponin, protodioscin, in the seeds and aerial parts of B. brizantha and B. decumbens during different developmental stages (growth, bloom, fructification and seed fall). The butanolic fraction of the ethanolic extract of each stage was submitted to thin layer chromatography (TLC) and spectrophotometric analysis through the Ehrlich reagent in 515nm. The chromatograms in TLC of the butanolic fraction of B. brizantha and B. decumbens showed similar spots as the protodioscin standard. The estimated level of protodioscin isomers in B. brizantha and B. decumbens ranged from 0.5% to 2.1%, having the highest level at the end of their developmental stages during seed falling comparison with the previous one. Protodioscin was not detected in the seeds. Outbreaks of Brachiaria spp. poisoning in central Western Brazil are frequently observed in pastures that had been more than 30 days without animals grazing, and also during the growing or blooming stage of the pastures. Other saponin determinations in toxic and non toxic pastures are necessary to determine the saponin concentrations that cause intoxication.

scholarly journals ISOLATION AND ANALYSIS OF STEROIDAL SAPONINS FROM POLYGONATUM ODORATUM (MILL.) DRUCE

2021 ◽  
Vol 55 ◽  
pp. 135-140
Author(s):  
Cristina MOGOSAN ◽  
Ilioara ONIGA ◽  
Mircea TAMAS
Keyword(s):  
Thin Layer ◽  
Acid Hydrolysis ◽  
Thin Layer Chromatography ◽  
Biological Properties ◽  
Steroidal Saponins ◽  
Physico Chemical ◽  
Layer Chromatography ◽  
Hydrolysis Of

We isolated the steroidal saponins from the rhizomes of Polygonatum odoratum (Mill.) Druce with an efficiency of 4.50% which represents 7 fractions identified by thin-layer chromatography (TLC), of which 3 were furostanics and 4 spirostanics. After the acid hydrolysis of the saponins, one aglycone (sapogenine) was identified by TLC. Further, we have determined the physico-chemical and the biological properties of the isolated saponins.

scholarly journals Comparison of Antioxidant Activity and Main Active Compounds Among Different Parts of Alpinia officinarum Hance Using High-Performance Thin Layer Chromatography-Bioautography

2019 ◽  
Vol 102 (3) ◽  
pp. 726-733 ◽  
Author(s):  
Wan-Xin Zhang ◽  
In-Cheng Chao ◽  
De-jun Hu ◽  
Farid Shakerian ◽  
Liya Ge ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Quality Control ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Aerial Parts ◽  
Alpinia Officinarum ◽  
Layer Chromatography ◽  
Different Parts ◽  
Tof Ms

Abstract Background: Alpinia officinarum Hance (ginger family) is an important Chinese medicine, especially in Southern China. Objective: A simple and effective high-performance thin-layer chromatography coupled with 2, 2-diphenyl-1-picrylhydrazyl bioautography (HPTLC-DPPH) and electrospray ionization quadrupole time-of-flight tandem mass spectrometry (ESI-Q-TOF-MS/MS) method was developed for the bioactivity-based quality control of A. officinarum. Methods: The HPTLC-DPPH and ESI-Q-TOF-MS/MS were applied for the analysis of different parts of A. officinarum after using methanol extraction for 23 batches of taproot, four batches of aerial, and three batches of fibril parts. Results: The systematic evaluation showed that similar components in taproot and aerial parts make the major antioxidant activity. However, based on our evaluation, the antioxidant ability of the aerial parts is lower than the taproot parts. There is also a significant difference (P < 0.05) between taproot and fibril parts of the root. The chemical structures of compounds with the antioxidant capacity were tentatively identified as 5R-hydroxy-7-(4-hydroxy-3-methoxyphenyl)-1-phenyl-3-heptanone (band 1), kaempferide (band 2), and galangin (band 3) based on ESI-Q-TOF-MS/MS analytical results and further confirmed by standards. Conclusions: This identification indicated that two flavonoid compounds and one diarylheptanoid compound possessed high potentials to be used as the antioxidant biomarkers for the quality control of A. officinarum. Highlights: The comparison of different parts could be considered as guidelines for the usage of A. officinarum.

scholarly journals Phytochemical Investigation of the Aerial Part of Iraqi Convolvulus arvensis

2020 ◽  
Vol 29 (2) ◽  
pp. 62-69
Author(s):  
Mustafa H. Alwan ◽  
Maha N. Hamad
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
Mass Spectroscopy ◽  
Morning Glory ◽  
Convolvulus Arvensis ◽  
Aerial Parts ◽  
Phytochemical Investigation ◽  
Layer Chromatography

  Convolvulus arvensis is a species of bindweed that is rhizomatous and is in the morning glory family (Convolvulaceae) native to Europe and Asia. The plant is naturally grown in Iraq. The plant was reported to be used in traditional medicine from as early as 1730s. The Aerial parts of Convolvulus arvensis were macerated in 80% ethanol for 6 days. The concentrated extract was partitioned with n-hexane, chloroform, ethyl acetate- and n-butanol successively. The n-hexane and ethyl acetate, fractions were examined for the presence of phytochemicals by thin layer chromatography and high performance liquid chromatography and its steroid and flavonoid contents were investigated. Stigmasterol was isolated from n-hexane fraction and identified by liquid chromatography/mass spectroscopy. Rutin was isolated from the ethyl acetate fraction and identified by liquid chromatography/mass spectroscopy. The aim is to examine the phytochemical constituents of the aerial parts of Convolvulus arvensis, literature survey available so far revealed that there were no studies about the phytochemical investigation for Convolvulus arvensis in Iraq.  Different chromatographic techniques like Thin Layer Chromatography and mass spectroscopy were used and the presence of Stigmasterol and Rutin in aerial parts of Convolvulus arvensis was indicated.  

scholarly journals Pharmacognostical, Physicochemical and Preliminary Phytochemical Standardization of Aerial Parts of Onosma bracteatum Wall

2017 ◽  
Vol 9 (6) ◽  
Author(s):  
Patel V. G. ◽  
Patel K. G. ◽  
Patel K. V. ◽  
Gandhi T. R.
Keyword(s):  
Phenolic Compounds ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Microscopical Examination ◽  
Phytochemical Screening ◽  
Aerial Parts ◽  
Inorganic Acids ◽  
Physicochemical Evaluation ◽  
Thin Walled ◽  
Layer Chromatography

Objective: The present study was undertaken to develop the standardization parameters of powdered aerial parts of Onosma bracteatum Wall, family Boraginaceae. Methods: Different parameters such as pharmacognostical, physicochemical, preliminary phytochemical evaluation along with thin layer chromatography for identification of phytoconstituents were studied. Results: On microscopical examination of the aerial parts it showed the presence of oval to polygonal thin walled straight epidermal cells; spiral vessels, a few fibres elongated with blunt tips, long warty, tubercle based unicellular hairs and paracytic stomata. On physicochemical evaluation it was found to contain more amount of polar constituents as the ethanol extractive value was found to be more. Total ash value and acid insoluble ash indicated the presence of inorganic acids and silicaceous matter respectively. Foaming index and swelling index were indicative of saponins and mucilaginous matter present in the aerial parts. On preliminary phytochemical screening and thin layer chromatographic studies it revealed the presence of saponins, flavonoids, phenolic compounds and mucilage. Conclusion: The present work carried out can serve as a purpose for identification, authentication and standardization of the crude drug.

scholarly journals ANTIBACTERIAL ACTIVITY OF ETHANOLIC EXTRACT AND INFUSE OF Bauhinia variegata LEAVES AGAINST Streptococcus pyogenes

2018 ◽  
Vol 14 (1) ◽  
pp. 1-7
Author(s):  
Istiqamatush Sholihah
Keyword(s):  
Antibacterial Activity ◽  
Thin Layer ◽  
Streptococcus Pyogenes ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Ornamental Plant ◽  
Test Method ◽  
Distilled Water ◽  
Bauhinia Variegata ◽  
Layer Chromatography

Bauhinia variegata is a plant that has many virtues and widely grown in Indonesia. Yet the virtues and uses are still limited as the shade and ornamental plant. The purpose of this study is to determine the antibacterial ethanol extract activity and the infuse of Bauhinia Variegata leaves toward Streptococcus pyogenes and to determine the classes of compounds that have antibacterial activity. Bauhinia variegata leaves are extracted by maceration and infundation with 96% ethanol and distilled water. The test method of antibacterial activity is used disc difusson Kirby Bauer methods. The Analyses of the compounds which are contained in the Bauhinia Variegata leaves are done by TLC (Thin Layer Chromatography) and test tubes. The results showed that ethanol extract and infuse of Bauhinia Variegata leaves has no antibacterial activity toward Streptococcus pyogenes. TLC results and test tubes showed that the ethanol extract of Bauhinia Variegata leaves contain tannins, saponins, terpenoids, and alkaloids. While, the infuse of Bauhinia variegata leaves are saponins and alkaloids. Keywords: Bauhinia variegata, antibacterial, Streptococcus pyogenes, maceration, infundation.

scholarly journals EFEKTIVITAS EKSTRAK ETANOL DAUN MAHONI (Swietenia mahagoni (L.) Jacq.) TERHADAP LARVA Aedes aegypti L.

2017 ◽  
Vol 4 (2) ◽  
pp. 23
Author(s):  
Tisa Rizkika Nur Amelia
Keyword(s):  
Secondary Metabolites ◽  
Aedes Aegypti ◽  
Thin Layer ◽  
Key Words ◽  
Thin Layer Chromatography ◽  
Ethanolic Extract ◽  
Botanical Insecticide ◽  
Flavonoid Compounds ◽  
Layer Chromatography ◽  
Swietenia Mahagoni

<p>The aim of the research were to evaluate the efficacy of botanical insecticide of <em>S. mahagoni </em>leaves extracts against larvae of <em>Ae. aegypti,</em> based on concentration of the leaves <em>S. mahagoni </em>extract, and in additional to determine secondary metabolites compounds of <em>S. mahagoni </em>leaves extract. The extraction of <em>S. mahagoni </em>leaves was done by using ethanol solvents and than was analyzed by using Thin Layer Chromatography. The result indicated that ethanolic extract of <em>S. mahagoni </em>leaf contained alkaloid, tannin, saponin, terpenoid, and flavonoid compounds. The value of LC<sub>50 </sub>and LC<sub>90</sub> calculation showed that LC<sub>50</sub> of ethanolic extract over second and third instar larvae respectively were 488 ppm and 644 ppm. However the value of LC<sub>90</sub> of both instar larvae were 732 ppm and 797 ppm. Based on the above result, it can be concluded that ethanolic extract of <em>S. mahagoni </em>leaf was effective against larvae of <em>Ae .aegypti</em>.</p><p>Key words: <em>Ae. aegypti</em>, <em>S. mahagoni</em>, botanical insecticide</p>

scholarly journals TO QUANTIFY THE LUTEOLIN CONTENT FROM THE AERIAL PARTS OF HETEROPOGON CONTORTUS (L.) BEAUV. (SPEAR GRASS) THROUGH HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY

2018 ◽  
Vol 11 (1) ◽  
pp. 191 ◽  
Author(s):  
Navjot Kaur ◽  
Raghbir Chand Gupta
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
Inflammatory Diseases ◽  
Leaf Sample ◽  
Chromatographic Fingerprint ◽  
Marker Compound ◽  
Heteropogon Contortus ◽  
Aerial Parts ◽  
Rf Values ◽  
Layer Chromatography

  Objective: The objective of this study was to quantify the luteolin content present in the extracts from different aerial plants (leaves, stem, and inflorescence) of Heteropogon contortus through high-performance thin-layer chromatography.Methods: The chromatographic fingerprint analysis of the different plant extracts has been developed using optimized mobile phase toluene: ethyl acetate: formic acid (5:5:0.7 v/v) and the developed plate is derivatized with freshly prepared anisaldehyde-sulfuric acid. Then, the plate is heated at 110–120°C. The plate is scanned for densitometry measurements and to record the overlay spectra at 366 nm absorbance/reflectance wavelength. Quantification of luteolin marker compound in different extracts of H. contortus is estimated using 2–12 ng/spot.Results: The yellow colored bands appearing on the chromatogram confirm the presence of luteolin marker compound in the different plant samples of H. contortus. Further, the presence of the luteolin marker is confirmed by comparing the Rf values (0.21) of the standard and the samples and from densitometry measurements by scanning the plate at 366 nm absorbance/reflectance. Line-to-line overlay spectra are obtained.Conclusion: From this, it is concluded that leaf sample of H. contortus contains maximum amount of luteolin, i.e., 37.13 ± 0.11 mg/g of dry wt. than inflorescence (1.60 ± 0.013 mg/g of dry wt.) and stem (0.53 ± 0.014 mg/g of dry wt.). The leaves are good source of luteolin and can be used as an alternate natural source to synthesize herbal drugs to cure cancer, hypertension, and inflammatory diseases. 

scholarly journals Thin-Layer Chromatography-Densitometry and Liquid Chromatography Analysis of Alkaloids in Leaves of Papaver somniferum Under Stress Conditions

2005 ◽  
Vol 88 (5) ◽  
pp. 1571-1578 ◽  
Author(s):  
Beata Szabó ◽  
Ágnes Lakatos ◽  
Tamás Kõszegi ◽  
György Kátay ◽  
L Botz
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Developmental Stages ◽  
Papaver Somniferum ◽  
Stress Conditions ◽  
Chromatography Method ◽  
Detection Techniques ◽  
Layer Chromatography ◽  
Alkaloid Contents

Abstract The effect of stress conditions on the concentrations of secondary metabolites were examined during various developmental stages of Papaver somniferum plants. P. somniferum plants were grown in laboratory conditions (Budakalász). The experiment consisted of 22 treatments. Significantly different alkaloid contents can be observed under different stress conditions. In general, the alkaloid contents of plants are very low; therefore, a highly sensitive and reliable method has to be developed for analysis. The amount of alkaloids was measured by 2 separation and detection techniques. Accuracy of the thin-layer chromatography method for quantitative analysis is limited. Without purification of samples the background is too noisy. Column liquid chromatography is a sensitive and relatively inexpensive method that allows precise quantitative determination of the alkaloid content.

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