Lumbar spine stability following hemilaminectomy, pediculectomy, and fenestration

2000 ◽  
Vol 13 (04) ◽  
pp. 165-171 ◽  
Author(s):  
A. M. Lubbe ◽  
A. J. Guthrie ◽  
T. P. Hill
Keyword(s):  
Lumbar Spine ◽  
Lumbar Vertebrae ◽  
Bending Moment ◽  
In Vitro Study ◽  
Control Group ◽  
Lateral Bending ◽  
Angular Deformation ◽  
Group A ◽  
The Common

SummaryThe destabilising effect of the removal of a single vertebral diarthrosis and the ipsilateral incision of the anuliis fibrosus at two adjacent vertebrae in the canine lumbar spine was quantified in an in vitro study. Lumbar spine segments, from the first to fourth lumbar vertebrae, were harvested from canine cadavers and divided into 1 control group (A) and 3 experimental groups - hemilaminectomy and fenestration (B), pediculectomy and fenestration (C), fenestration alone (D). Samples were subjected to 4 point lateral bending to point of failure. Stiffness, maximum bending moment (Mbmax), angular deformation and bending moment at 15° (Mb at 15°) variables were determined for each group. Hemilaminectomy and fenestration had the greatest decrease in Mbmax, Mb at 15° and stiffness and the greatest increase in angular deformation. Pediculectomy and fenestration and the fenestration alone procedures caused similar and significant reductions in Mb at 15° and stiffness. Pediculectomy was less destabilizing than hemilaminectomy, although this difference was not statistically significant. Fenestration is the common and greatest destabilizing factor. The removal of a single pedicle and accessory process has minimal destabilizing effects.The destabilizing effect of hemilaminectomy, pediculectomy and fenestration were quantified and compared in a study using canine cadaver spines. The combination of hemilaminectomy and fenestration produced the greatest degree of instability, but fenestration was the shared, most significant, single destabilizing factor.

scholarly journals Hydroxyl and calcium ions diffusion from endodontic materials through roots of primary teeth - in vitro study

2005 ◽  
Vol 13 (2) ◽  
pp. 187-192 ◽  
Author(s):  
Ana Cristina Gerent Petry Nunes ◽  
Maria José de Carvalho Rocha
Keyword(s):  
In Vitro Study ◽  
Deciduous Teeth ◽  
Control Group ◽  
Apical Surface ◽  
Working Length ◽  
Group A ◽  
Periapical Healing ◽  
Materials Used ◽  
Spectrometer Data

The purpose of this research was to evaluate the diffusion of calcium (Ca+2) and hydroxyl (OH-) ions from materials with a calcium hydroxide base - Ca(OH)2 through the intact roots of deciduous teeth. This diffusion of ions is important for periapical healing. Forty-six deciduous teeth were selected and instrumented to their working length with #40 files. The teeth were washed during cleaning and shaping with a 1% sodium hypochlorite (NaOCl) solution. The canals were dried with paper points. The teeth were divided into 4 groups based on the sealer type, with 10 specimens in each group. A fifth group of 6 teeth without sealer constituted the control group. The materials used as sealers were: Ca(OH)2 paste associated to propylene glycol (CaPE) thickened at the proportion of 2:1 w/v; UFSC (Federal University of Santa Catarina) paste - a mixture of 0.3g of zinc oxide with 0.3g of Ca(OH)2 with 0.2ml of olive oil 1:1 w/w; Vitapex® and Sealapex®. The coronal access was sealed with a glass ionomer after the root had been filled with each sealer. A one-third apical surface and foramen was hard-pressed with Araldite®. The teeth were stored individually in flasks containing saline solution at 37ºC and 100% humidity. The OH- and Ca+2 ions diffusion levels were determined using a pH meter and an atomic absorption spectrometer. Data were collected at 48 hours and at intervals of 7, 30, 45 and 60 days. Statistical analysis was performed using ANOVA to compare groups. In the pH evaluation, the CaPE group presented the largest OH- ions diffusion, which peaked at sixty days (p=0.0309), when compared to the other groups (p<0.0001). In relation to amount of Ca+2 ions released, the CaPE paste showed the best results, followed by the UFSC's paste. These results suggest that the CaPE paste was the material that allowed the highest diffusion of OH- and Ca+2 ions.

EVALUATION OF THE EFFECT OF FERROUS SULFATE ON ENAMEL DEMINERALIZATION OF HUMAN DECIDUOUS TEETH: AN IN VITRO STUDY

2017 ◽  
Vol 8 (3) ◽  
pp. 83-89
Author(s):  
Johnny Holanda De Gauw ◽  
Lara Maria Melo Costa ◽  
Rodrigo Neves Silva ◽  
Natanael Barbosa Santos ◽  
Maria Dânia Holanda Tenorio
Keyword(s):  
Ferrous Sulfate ◽  
Polarized Light ◽  
In Vitro Study ◽  
Deciduous Teeth ◽  
Control Group ◽  
Group A ◽  
Ph Cycling ◽  
Group B ◽  
Group D

Objective: This study aimed to evaluate the effect of ferrous sulfate (FS) on demineralized and non-demineralized human deciduous teeth. Additionally, it was evaluated the penetration extent of FS and its remineralizing effect on the enamel of deciduous teeth using Polarized Light Microscopy (PLM). Method: The sample comprised 44 human deciduous teeth. The 44 crowns were divided randomly into four groups: group A (FS after demineralization), group B (FS without demineralization), group C (only demineralization), and group D (control group). FS at 0.45 mol/L-1 was used daily (15 days) and demineralization was done by pH cycling (7 days). Then, three longitudinal slices of the crowns were photographed using PLM. The degree of penetration of the lesion or stain was measured in micrometers, as well as the distance between the external enamel surface and the core of lesion. Results: Group A showed a dark stain on the outer surface of enamel larger than the group B. It is suggested, a remineralizing effect when comparing groups, A and C. The mean depth and standard deviation for groups A, B, and C were 4.27µm (±1.49), 3.72 µm (±1.68) and 5.00 µm (±1.84), respectively. No dark stains were observed in group D. Conclusion: FS stained the demineralized and non-demineralized human deciduous teeth. However, dark stains in the non-demineralized teeth were smaller or absent, than in the demineralized teeth. Therefore, FS may have a protective effect against demineralization.

scholarly journals Effect of Honey in the Management of Otomycosis

2017 ◽  
Vol 8 (2) ◽  
Author(s):  
Amisha Patel ◽  
Vaghela D B
Keyword(s):  
Hearing Loss ◽  
Intervention Group ◽  
In Vitro Study ◽  
Signs And Symptoms ◽  
Control Group ◽  
Humid Climate ◽  
Group A ◽  
Clinical Control Trial ◽  
Group B

Otomycosis is a fungal infection of External ear, characterized by earache, pruritus, ear blockage, discharge, hearing loss and ringing in ear.  It is the most common clinical entity in the ENT clinics. The disease is more common in hot and humid climate. Its worldwide prevalence is 5.2 % and 9 % in India. 5-25 % of otitis externa cases are due to otomycosis. Bactericidal and fungicidal activity of Honey is proved previously by in vitro study. Honey has high osmolarity and acidic pH. And fungus does not grow in acidic media. With this background, present study is aimed to know the efficacy of the Honey in the management of Otomycosis. This randomized clinical control trial including two groups, one is intervention group (group A) and second is control group (group B). Pt is advised to instill three drops of Honey thrice a day for 7 days in Group A and three drops of Clotrimazole thrice a day for 7 days in Group B. There is statistically highly significant results was observed in all signs and symptoms except hearing loss which is significant in both Groups. Statistically insignificant difference was found between both Groups. 

scholarly journals Zeolite as a Bone Bio-Modifier Carrier: An In-Vitro Study

2019 ◽  
Vol 9 (1) ◽  
pp. 126
Author(s):  
Mobina Mousavi ◽  
Azadeh Esmaeil Nejad ◽  
Erfan Shamsoddin ◽  
Mohammad Mehdi Golabgiran ◽  
Behzad Houshmand
Keyword(s):  
In Vitro Study ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Alizarin Red ◽  
Group A ◽  
Group B ◽  
And Function ◽  
Group D

Background: Zeolite is a microporous aluminosilicate compound which has been successfully used in tissue engineering. The effects of Zeolite on the morphology and functions of pre-osteoblastic MG-63 cells as new bone enhancer material is still unclear. Methods: In this vitro experimental study, MTT and Alizarin red staining test were performed on six groups of MG-63 cells which differed in Zeolite (Z) concentration and the presence or absence of Alloplast extract (A). Group A: 0.1&mu;g/mL Z+A, Group B: 0.1&mu;g/mL Z without A, Group C: 0.2&mu;g/mL Z+A, Group D: 0.2&mu;g/mL Z without A, Group E: 0.3&mu;g/mL Z+A, Group F: 0.3&mu;g/mL Z without A. There were also three control groups as positive control, negative control, and Alloplast control based on each related test. The data were analyzed by SPSS 20 via one-way ANOVA and Welch test. (P&lt;0.05). Results: At 24 hours, results showed that solutions with 0.1&mu;g/mL, 0.2&mu;g/mL, and 0.3&mu;g/mL Zeolite with or without Alloplast had significantly higher proliferation rates than positive control (distilled water) groups without Alloplast (p&lt;0.001). At 72hours time point, the results showed significantly higher proliferation rates in the solutions with 0.1&mu;g/mL, 0.2&mu;g/mL, and 0.3&mu;g/mL Zeolite with or without Alloplast compared to the positive control group without Alloplast (p&lt;0.001). Conclusions: Zeolite can increase proliferation of MG-63 cells without presence of Alloplast; It seems that combination of Zeolite with Alloplast maybe enhancing proliferation and function of MG-63 cells.

Sealing Ability of ProRoot MTA and MTA-Angelus Simulating a One-Step Apical Barrier Technique- An in vitro Study

2009 ◽  
Vol 33 (4) ◽  
pp. 305-310 ◽  
Author(s):  
Nikita Lolayekar ◽  
Sham Bhat ◽  
Sundeep Hegde
Keyword(s):  
Calcium Hydroxide ◽  
In Vitro Study ◽  
Control Group ◽  
Blue Dye ◽  
Root Segments ◽  
Sealing Ability ◽  
Significant Difference ◽  
Group A ◽  
Group B

Endodontic treatment of the pulpless tooth with an immature root apex poses a special challenge for the clinician due to lack of an apical stop against which to compact an interim dressing of calcium hydroxide, or the final obturation material. A one-visit apexification protocol with MTA (Mineral Trioxide Aggregate) has be seen as an alternative to the traditional treatment practices with calcium hydroxide [Ca(OH)2]. The aim of this study was to investigate the use of MTA as a 5mm apical barrier by comparing sealing ability of ProRoot MTA to that of MTA-Angelus. 50 freshly extracted Maxillary and Mandibular single canal sound human incisor teeth were used in this study, which were decoronated to standardized 10mm root lengths. The root segments were prepared to simulate the clinical situation of an open apex with Gates Glidden burs # 5-1 and randomly assigned into 2 experimental groups of 20 samples each (Group A: ProRoot MTA, Group B: MTA-Angelus) and 10 root segments were used as control (Group C) where no apical barrier was used. After obturation the coronal portion of all samples were sealed with Glass Ionomer and stored at 37°C and 100% humidity for 4 weeks. The root segments were then double coated with nail varnish except for the apical 1mm and apical ends of all root segments were suspended vertically in methylene blue dye for 48 hours at room temperature. After removal from the dye, the samples were sectioned longitudinally and linear dye penetration was measured using a Grid and stereomicroscope. The measurements were tabulated and statistically analyzed. The mean percentage of dye leakage for Group A was 55.5% and for Group B was 53.25%. The results showed no statistically significant difference in the sealing ability of ProRoot MTA and MTA-Angelus when used as apical barriers.

Enameloplasty effects on microleakage of pit and fissure sealants under load: an in vitro study

2000 ◽  
Vol 24 (4) ◽  
pp. 279-285 ◽  
Author(s):  
Christina Zervou ◽  
Gerald Kugel ◽  
Cataldo Leone ◽  
Athanasios Zavras ◽  
Eileen Doherty ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Conventional Technique ◽  
Control Group ◽  
Fissure Sealants ◽  
Occlusal Force ◽  
Pit And Fissure Sealants ◽  
Significant Difference ◽  
Group A ◽  
Group D

Enameloplasty has been implicated in the successful application of pit and fissure sealants. The present study aimed to evaluate the effect of enameloplasty technique on microleakage of sealants when occlusal force was applied on the teeth. The study also allowed a direct comparison of the effectiveness of enameloplasty, when two different burs were used. Six groups of ten human extracted wisdom teeth were tested. Group A: no load, conventional (no enameloplasty - control); Group B: no load, enameloplasty 1/2 round bur (Brasseler USA); Group C: no load, enameloplasty diamond fissure bur REF/UP 791( Ultradent); Group D: load 500 N, conventional , Group E: load 500 N, 1/2 round bur; Group F: load 500 N, diamond fissure bur. Specimens were thermocycled for 500 cycles at 5° and 55° with a dwell time of 30 seconds after load application. Microleakage was scored as distance of dye penetration with 0 = no microleakage and 3 = microleakage to underling fissure. The Kruskal-Wallis One Way Anova and Mann -Whitney U test showed group D having the greatest degree of microleakage statistically significant (p&lt;0.05) comparing with other groups. Sealants prepared with the conventional technique (A, D) had statistically significant differences in microleakage with those prepared with enameloplasty (B,C,E, F) (p = 0.01). Enameloplasty in groups with no load (B, C) did reduce microleakage, but not significantly (p = 0.3) However, loaded teeth with enameloplasty appeared to perform superior, as compared to those without (p=0.005) Regarding the effect of load, teeth without load (Groups A,B, C) were found to have significantly less microleakage than teeth where mechanical force had been applied (Groups D, E, F), (p = 0.01). Microleakage in Group A (conventional technique without load) was significantly less than microleakage in Group D (conventional technique with load), (p = 0.04). However, load did not seem to influence microleakage when enameloplasty had been performed. This finding was true for both round bur enameloplasty (p = 0.29), and fissure bur enameloplasty (p = 0.26). There was no statistically significant difference between Groups B, E (round bur) and C, F (fissure bur) (p &gt; 0.05), or between Groups B and C (p &gt; 0.05) and between Groups E and F (p &gt; 0.05). The results of the study indicated that enameloplasty reduced microleakage of pit and fissure sealants, especially when load was applied to teeth, irrespective of what bur was used to enlarge the fissure, as there was no statistical significant differences between the round and fissured diamond burs. The application of occlusal force to the tooth produces significantly more microleakage, unless enameloplasty is performed.

Caries protective effect of two Self-etch dentin bonding agents on denuded root dentin surface: In Vitro Study

2019 ◽  
Vol 11 (2) ◽  
pp. 36-41
Author(s):  
Dr. Jini P M ◽  
Dr. Shibani Shetty ◽  
Dr. Jayalakshmi K B ◽  
Dr. Prasannalatha Nadig ◽  
Dr. Sujatha I
Keyword(s):  
Protective Effect ◽  
In Vitro Study ◽  
Bonding Agent ◽  
Control Group ◽  
Bonding Agents ◽  
Dental Adhesive ◽  
Dentin Bonding ◽  
Group A ◽  
Root Dentin

Objective: To evaluate the caries protective effect of two self-etching dental adhesive agents on the denuded root surface. Materials and methods: The roots of 30 freshly extracted , non-carious human premolars were cleaned and denuded of cementum. The teeth surfaces were covered with acid-resistant nail varnish, exposing two rectangular windows (lingual and buccal surface) of 4 x 4 mm each. The window on the lingual furface served as the control , while the buccal window were treated with dentin bonding agent. All specimens were store in acidifed gel for 6 days for demineralization. The specimens were then divided among two experimental groups-Group A: Optibond all in one and Group B: Bond-1SF.In Group C (control group) bonding agent was not applied. The specimens were stored in 0.9% sodium chloride solution for 14 days. Plano parallel tooth sections (80 ± 20 μm) were obtained. Tooth sections were evaluated for caries‑like demineralized zone under a polarized microscope. The values obtained were analyzed using one‑way ANOVA and Tukey honesty tests. Results: In the Control group the mean depth of demineralization was 80.08µm .The depth of demineralization was significantly reduced in Group A and B. Conclusion: Self-etching dental adhesive agents with and without solvent is an effective method to prevent demineralization of root dentin.

Reverse torque values of abutment screws after dynamic loading: The effects of sealant agents and the taper of conical connections

2020 ◽  
Author(s):  
Arda Ozdiler ◽  
suleyman dayan ◽  
Burc Gencel ◽  
Gulbahar Isık-Ozkol
Keyword(s):  
Dynamic Loading ◽  
Antibacterial Agent ◽  
In Vitro Study ◽  
Control Group ◽  
Silicone Sealant ◽  
Reverse Torque ◽  
The Stability ◽  
Torque Values ◽  
Chlorhexidine Gel

This in vitro study evaluated the influence of taper angles on the internal conical connections of implant systems and of the application of chlorhexidine gel as an antibacterial agent or a polyvinyl siloxane (PVS) sealant on the reverse torque values of abutment screws after dynamic loading. The current study tested four implant systems with different taper angles (5.4°, 12°, 45°, and 60°). Specimens were divided into three groups: control (neither chlorhexidine gel filled nor silicone sealed), 2% chlorhexidine gel-filled or silicone-sealed group, and group subjected to a dynamic load of 50 N at 1 Hz for 500,000 cycles prior to reverse torque measurements. Quantitative positive correlation was observed between the taper angle degree and the percentage of tightening torque loss. However, this correlation was significant only for the 60° connection groups except in the group in which a sealant was applied ( p = 0.013 for the control group, p = 0.007 for the chlorhexidine group). Percentages of decrease in the torque values of the specimens with silicone sealant application were significantly higher compared with both the control and chlorhexidine groups ( p = 0.001, p = 0.002, p = 0.001, and p = 0.002, respectively, according to the increasing taper angles); the percentage of decrease in torque values due to chlorhexidine application was statistically insignificant when compared with the control group. The application of gel-form chlorhexidine as an antibacterial agent does not significantly affect the stability of the implant–abutment connection under dynamic loads. PVS sealants may cause screw loosening under functional loads.

Comparative Study to Evaluate the Surface Detail Reproduction of Dental Stone after Immersion in Various Different Disinfectant Solutions, Under Stereomicroscope 10 X Magnification –An In-Vitro Study

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
Rathika Rai ◽  
M. A. Easwaran ◽  
K. T. Dhivya
Keyword(s):  
Sodium Hypochlorite ◽  
In Vitro Study ◽  
Control Group ◽  
Immersion Method ◽  
Significant Difference ◽  
Surface Irregularities ◽  
Disinfectant Solution ◽  
Surface Detail ◽  
Group B

Aim: To evaluate the surface detail reproduction of dental stone this is immersed in different disinfectant solution and studied under stereomicroscope. Methodology: Total number of 30 specimens of dental stone (Type III) were made with measurements of 1.5cm diameter and 1cm height .This samples are divided in to 3 groups group A,B,C. were A is immersed in Distilled water which was taken as control group ;B is immersed in 2% Glutaraldehyde and C is immersed in 5%sodium hypochlorite. Each specimen were immersed in the disinfectant solution for 15 minutes and dried under room temperature for 24 hrs. After 24 hrs each specimens are studied under stereomicroscope for surface details. Result: The results showed no significant difference in the surface irregularities and porosities for a group 1 and group 2 except group 3 which showed significant increase in the porosities, surface irregularities and erosions after disinfection with 5% NaHOCl by immersion method. Conclusion: The surface detail reproduction capacity of die stone was adversely affected when 5% Sodium hypochlorite was used as disinfectant solution when compare d to control group and 2% Glutaraldehyde

scholarly journals Pomegranate Peel Extract Is a Potential Alternative Therapeutic for Giardiasis

Antibiotics ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 705
Author(s):  
Asmaa M. El-Kady ◽  
Iman A. M. Abdel-Rahman ◽  
Samer S. Fouad ◽  
Khaled S. Allemailem ◽  
Taghrid Istivan ◽  
...  
Keyword(s):  
Diarrheal Disease ◽  
Apoptotic Cell ◽  
In Vitro Study ◽  
Ethanolic Extract ◽  
Control Group ◽  
Pomegranate Peel ◽  
Pomegranate Peel Extract ◽  
Multiple Drugs ◽  
Peel Extract

Giardiasis is a major diarrheal disease affecting approximately 2.5 million children annually in developing countries. Several studies have reported the resistance of Giardia lamblia (G. lamblia) to multiple drugs. Therefore, identifying an effective drug for giardiasis is a necessity. This study examined the antiparasitic effect of Punica granatum (pomegranate) and evaluated its therapeutic efficacy in rats infected with G. lamblia. In vitro study showed high efficacy of pomegranate peel ethanolic extract in killing G. lamblia cysts as demonstrated by eosin vital staining. We showed that treating infected rats with pomegranate extract resulted in a marked reduction in the mean number of G. lamblia cysts and trophozoites in feces and intestine respectively. Interestingly, the number of G. lamblia trophozoites and cysts were significantly lower in the pomegranate extract-treated group compared to the metronidazole-positive control group. Moreover, pomegranate extract treatment significantly induced nitric oxide (NO) and reduced serum IL-6 and TNF-α, compared to infected untreated rats. Histological and scanning electron microscopy (SEM) examination of the jejunum and duodenum of pomegranate extract-treated animals confirmed the antiparasitic effect of the extract, and demonstrated the restoration of villi structure with reduction of villi atrophy, decreased infiltration of lymphocytes, and protection of intestinal cells from apoptotic cell death. In conclusion, our data show that the pomegranate peel extract is effective in controlling G. lamblia infections, which suggests that it could be a viable treatment option for giardiasis.

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