scholarly journals Seed longevity, viability and germination of four weed-ruderal Asteraceae species of ethnobotanic value

2021 ◽  
Vol 99 (2) ◽  
pp. 279-290
Author(s):  
Andrea Mariel Jiménez-Vázquez ◽  
Alejandro Flores-Palacios ◽  
Alejandro Flores-Morales ◽  
Irene Perea-Arango ◽  
María del Carmen Gutiérrez ◽  
...  
Keyword(s):  
Room Temperature ◽  
Storage Temperature ◽  
Seed Viability ◽  
Biological Properties ◽  
Seed Longevity ◽  
Ex Situ ◽  
Cold Temperatures ◽  
Design Production ◽  
One Year ◽  
Germination Experiments

Background: A high proportion of Asteraceae species are considered weeds, some of them have recognizable biological properties. To design production protocols and ex situ seed conservation programs is necessary to determine the effect of storage temperature on seed viability and longevity. It is known that cold temperatures maintain seed viability and thus prolong seed longevity. Hypotheses: The seeds of Aldama dentata, Verbesina virgata, Stevia origanoides and Roldana barba-johannis stored at low temperature (5 ºC) will have greater longevity than when stored at room temperature (16.45 ± 1.94 oC) and will decrease their viability as they age. Study site and dates: Seeds of the four Asteraceae were collected (November-December 2015) in The National Park “El Tepozteco”, Morelos, Mexico and in the “Chamilpa” campus of the Universidad Autónoma del Estado de Morelos. Methods: The monthly germination experiments (11) were implemented with seeds stored under both temperatures, germination (%) and mean germination time were registered to estimate seed longevity. The seed viability stored at room temperature was evaluated with the tetrazolium test. Results: Germination and viability decreased with seed age. Along the experiment, seed germination was similar at both storage temperatures. A. dentata and S. origanoides showed the highest germination but A. dentata alone reached the major seed viability. The oldest seeds germinated faster. Conclusions: Seed longevity of all the species exceeded one year. For propagation purposes, it is recommended to use seeds between 8 and 9 months of age, when their germination and viability are the highest.

Seed Longevity and Germination Behaviour in Eugenia roxburghii

2021 ◽  
Vol 27 (3) ◽  
pp. 167-171
Author(s):  
Shareef Muhammed ◽  
Chitra Rajeswary ◽  
Anil Chandran
Keyword(s):  
Storage Temperature ◽  
Seed Viability ◽  
Seed Longevity ◽  
Ex Situ ◽  
Hermetic Storage ◽  
Germination Behaviour ◽  
Garden Plant ◽  
Relationship Of ◽  
The Relationship ◽  
And Storage

Eugenia roxburghii is an evergreen graceful shrub with a tremendous potential as garden plant. As a part of ex-situ conservation and popularization of the species, seed longevity was studied by understanding the relationship of seed viability with respect to different moisture contents and storage temperature. Seeds are recognized as recalcitrant, being desiccation as well as chilling sensitive. During hermetic storage, seeds stored at 300C/70%RH retained viability for about 5 months and 4 months in 200C/20% RH. Seeds can be best stored for five months in laboratory conditions.

scholarly journals Seed longevity of maize conserved under germplasm bank conditions for up to 60 years

2021 ◽  
Author(s):  
Filippo Guzzon ◽  
Maraeva Gianella ◽  
Jose Alejandro Velazquez Juarez ◽  
Cesar Sanchez Cano ◽  
Denise E Costich
Keyword(s):  
Seed Germination ◽  
Plant Genetic Resources ◽  
Seed Viability ◽  
Storage Conditions ◽  
Seed Longevity ◽  
Germplasm Bank ◽  
Significant Difference ◽  
Different Temperatures ◽  
Germination Experiments

Abstract Background and Aims The long-term conservation of seeds of plant genetic resources is of key importance for food security and preservation of agrobiodiversity. Nevertheless, there is scarce information available about seed longevity of many crops under germplasm bank conditions. Methods Through germination experiments as well as the analysis of historical monitoring data, we studied the decline in viability manifested by 1000 maize (Zea mays subsp. mays) seed accessions conserved for an average of 48 years at the CIMMYT germplasm bank, the largest maize seedbank in the world, under two cold storage conditions: an active (–3 °C; intended for seed distribution) and a base conservation chamber (–15 °C; for long-term conservation). Key Results Seed lots stored in the active chamber had a significantly lower and more variable seed germination, averaging 81.4 %, as compared with the seed lots conserved in the base chamber, averaging 92.1 %. The average seed viability detected in this study was higher in comparison with that found in other seed longevity studies on maize conserved under similar conditions. A significant difference was detected in seed germination and longevity estimates (e.g. p85 and p50) among accessions. Correlating seed longevity with seed traits and passport data, grain type showed the strongest correlation, with flint varieties being longer lived than floury and dent types. Conclusions The more rapid loss of seed viability detected in the active chamber suggests that the seed conservation approach, based on the storage of the same seed accessions in two chambers with different temperatures, might be counterproductive for overall long-term conservation and that base conditions should be applied in both. The significant differences detected in seed longevity among accessions underscores that different viability monitoring and regeneration intervals should be applied to groups of accessions showing different longevity profiles.

Seed storage and germination studies of Garcinia talbotii Raizada ex Santapau seeds - an endemic tree from Western Ghats of India

2016 ◽  
Vol 23 (2) ◽  
pp. 73-78
Author(s):  
T. Sabu ◽  
P.S. Shameer ◽  
Chitra Rajeswary ◽  
N. Mohanan ◽  
C. Anilkumar
Keyword(s):  
Ex Situ Conservation ◽  
Storage Temperature ◽  
Seed Viability ◽  
Seed Storage ◽  
Ex Situ ◽  
Hermetic Storage ◽  
Western Ghats Of India ◽  
Relationship Of ◽  
The Relationship ◽  
And Storage

Seeds of Garcinia talbotii remained viable hardly for two weeks in open room conditions. As part of ex-situ conservation of Garcinia talbotii, seeds longevity was studied since seeds are the main propagule. For this, the relationship of seed viability with respect to different moisture content and storage temperature were analyzed. Seed storage behaviour is also investigated. Being recalcitrant, seeds are desiccation sensitive as well as chilling sensitive. During hermetic storage of seeds at 300C /70 % RH seeds retained viability about 6 months.

scholarly journals Effect of storage temperature on the quality of kepok banana (Musa paradisiaca formatypica)

2021 ◽  
pp. 17-47
Author(s):  
Andi Nur Faidah Rahman ◽  
Victor Crystaline Muhammad ◽  
Februadi Bastian
Keyword(s):  
Shelf Life ◽  
Skin Color ◽  
Room Temperature ◽  
Storage Temperature ◽  
Chilling Injury ◽  
Soluble Solids ◽  
Total Acid ◽  
Cold Temperatures ◽  
Fruit Skin

Kepok bananas are processed bananas that have ABB genotype, the letter B indicates that banana kepok has a stronger resistance to disease and chilling injury in cold temperatures storage. Storage at cold temperatures can extend the shelf life of bananas, because cold temperatures can slow down the respiration and enzymatic processes. The purpose of this study is to observe the effect of storage temperature on quality and shelf life, and to determine optimum storage temperature. The method used is storage temperature at room, 15oC and 10oC until the banana is damaged. The parameters observed were respiration patterns, weight loss, hardness, fruit skin color, total acid, vitamin C, pH, and total soluble solids. The results showed that bananas stored at cold temperatures (10oC and 15oC) can last up to 20 days while at room temperature only lasts for 10 days. Kepok bananas stored at 10oC have not shown symptoms of chilling injury.

One-step fitting of seed viability constants for two Australian plant species, Eucalyptus erythrocorys (Myrtaceae) and Xanthorrhoea preissii (Xanthorrhoeacea)

2013 ◽  
Vol 61 (1) ◽  
pp. 1 ◽  
Author(s):  
A. D. Crawford ◽  
F. R. Hay ◽  
J. A. Plummer ◽  
R. J. Probert ◽  
K. J. Steadman
Keyword(s):  
Survival Data ◽  
Seed Viability ◽  
Seed Storage ◽  
Storage Conditions ◽  
Gene Bank ◽  
Seed Longevity ◽  
Ex Situ ◽  
Australian Species ◽  
One Step

Long-term ex-situ seed storage under controlled conditions in gene banks has become an important tool for conserving threatened Australian plants; however, there is scant information about the seed longevity of most species. The aim of the present study was to determine whether the seed longevity of two contrasting Australian species could be modelled using the seed viability equation, and whether the universal temperature constants are applicable to these species. Seeds of Eucalyptus erythrocorys F.Muell. (Myrtaceae) and Xanthorrhoea preissii Endl. (Xanthorrhoeaceae) were aged at moisture contents ranging from 3.9 to 15.7% and temperatures between –20 and 60°C. Survival data were fitted to the seed viability equation in one step and the species constants for each species determined. Both E. erythrocorys and X. preissii seeds exhibited orthodox seed storage behaviour whose longevity could be modelled using the seed viability equation. The viability constants were KE = 8.81, CW = 4.97, CH = 0.0412 and CQ = 0.000379 for E. erythrocorys and KE = 8.77, CW = 5.29, CH = 0.0382 and CQ = 0.000473 for X. preissii. The universal temperature constants could not be used without a significant increase in error. The storage behaviour of these two Australian species is in keeping with that of orthodox species from around the world. Predictions are that E. erythrocorys will be long-lived under gene bank conditions, whereas X. preissii would be moderately long-lived. Current long-term gene bank storage conditions appear suitable for storage of these species; however, recommendations for short-term storage need to be re-evaluated.

scholarly journals Maturation of seeds of Poincianella pluviosa (Caesalpinoideae)

2015 ◽  
Vol 37 (2) ◽  
pp. 131-138 ◽  
Author(s):  
João Paulo Naldi Silva ◽  
Danilo da Cruz Centeno ◽  
Rita de Cássia Leone Figueiredo-Ribeiro ◽  
Claudio José Barbedo
Keyword(s):  
Room Temperature ◽  
Seed Viability ◽  
Dry Mass ◽  
Seed Vigor ◽  
Ex Situ ◽  
Environmental Constraints ◽  
Maturation Process ◽  
Short Lifespan ◽  
Wide Range ◽  
Viable Seeds

The persistence of viable seeds in the soil is an important way to assure plant propagation, especially for species which produce seeds with short lifespan. Poincianella pluviosa is a tree species which seeds have short to medium storability at room temperature. The comprehension of the maturation process is crucial to understand its strategy for propagation and it could provide tools to improve seed viability in ex situ conditions. Flowers were tagged in two consecutive cycles of maturation and pods were periodically harvested until dispersion. Seeds were classified based on their morpho-physiological features, capability to germinate and develop seedlings. The complete maturation process was attained 315-330 days after anthesis, a period longer than reported for seed viability at room temperature. The maximum dry mass and seed vigor were reached at the end of maturation, although elevated seedling production was obtained before physiological maturity. We suggest that the precocious ability to produce seedlings in a long maturation could be a strategy to overcome environmental constraints, as the species is distributed in a wide range of phytogeographic domains in Brazil.

Seed longevity and physical dormancy break of two endemic species of Dimorphandra from Brazilian biodiversity hotspots

2017 ◽  
Vol 27 (3) ◽  
pp. 199-205 ◽  
Author(s):  
Miele T. Matheus ◽  
Ailton G. Rodrigues-Junior ◽  
Denise M.T. Oliveira ◽  
Queila S. Garcia
Keyword(s):  
Cold Storage ◽  
Structural Changes ◽  
Seed Viability ◽  
Storage Conditions ◽  
Seed Longevity ◽  
Ex Situ ◽  
Biodiversity Hotspots ◽  
Physical Dormancy ◽  
Natural Habitats ◽  
Brazilian Biodiversity

AbstractSeed longevity is dependent on seed traits and storage conditions. This study evaluated the seed longevity and physical dormancy (PY)-break of two species of Dimorphandra endemic to Brazilian biodiversity hotspots. Longevity was tested in situ, by burying seeds in their natural habitats (12 months), and ex situ, by storage in a cold chamber (5°C; 24 months). Seeds were taken at regular intervals to assess germinability at 30°C (12 h photoperiod) using intact and scarified seeds. Intact seeds (freshly collected, and after 12 months storage) were analysed using scanning electron microscopy. The germinability of freshly collected seeds of both species reached approximately 10% for intact seeds and >85% for scarified seeds. Cold storage maintained seed viability in both species, and broke dormancy for 35% of D. wilsonii seeds. After 12 months, only 55% (for D. exaltata) and 41% (for D. wilsonii) of the buried seeds were recovered; more than 90% of which remained viable in both species. Seeds gradually overcame PY during burial, with a higher germination increase for D. wilsonii (71%) than D. exaltata (32%). Dimorphandra exaltata seeds did not show clear structural changes after cold storage although D. wilsonii seeds evidently experience an increase in the depth of fracture lines. Burial promoted deep seed coat changes in both species, more intense in D. wilsonii, indicating that temperature and humidity variations throughout the year are among the main factors releasing Dimorphandra seeds from PY. The seeds of both studied species overcame PY during burial and are able to form small persistent soil seed banks.

Seed mass and elevation explain variation in seed longevity of Australian alpine species

2018 ◽  
Vol 28 (4) ◽  
pp. 319-331 ◽  
Author(s):  
Annisa Satyanti ◽  
Adrienne B. Nicotra ◽  
Thomas Merkling ◽  
Lydia K. Guja
Keyword(s):  
Seed Mass ◽  
Seed Viability ◽  
Plant Conservation ◽  
Seed Longevity ◽  
Ex Situ ◽  
Accelerated Ageing ◽  
Alpine Regions ◽  
Australian Alps ◽  
Alpine Species

AbstractConserving alpine ecosystems and the plant communities they contain using ex situ conservation requires an understanding of seed longevity. Knowledge of seed longevity may determine the effectiveness of ex situ seed banking for alpine plant conservation, and may provide insight into plant recruitment in situ. We sought to determine the influence of elevation and climatic variables, as well as plant and seed traits, on the seed longevity of 57 species inhabiting a unique biome, (sub-)alpine regions of mainland Australia. Seed longevity was estimated using controlled accelerated ageing tests to determine the time taken for seed viability to fall by 50%. We found that, across the study species, like alpine seeds elsewhere in the world, Australian alpine seeds are relatively short-lived and overall shorter-lived than Australian plants in general. Seed mass and elevation explained most of the variation in seed longevity among the Australian alpine species considered. Species with larger seed mass, and collections made at higher elevations, were found to have relatively short-lived seeds. Phylogeny, however, explained very little of the variation in longevity. Our results suggest that viability testing for Australian alpine seeds in ex situ seed banks should be conducted with shorter intervals than for the non-alpine flora. This study highlights how seed longevity in the Australian Alps is not dictated primarily by evolutionary lineage but rather by a complex combination of environmental variables and intrinsic seed characteristics. Potential implications for conservation ex situ and in situ in the context of climate change are discussed.

scholarly journals EFIKASI CUKA KULIT PISANG DAN AIR KELAPA SEBAGAI PENGHAMBAT Listeria monocytogenes PADA DAGING AYAM

2017 ◽  
Vol 12 (2) ◽  
pp. 93
Author(s):  
Widaningrum Widaningrum ◽  
Miskiyah Miskiyah ◽  
Juniawati Juniawati
Keyword(s):  
Acetic Acid ◽  
Lactic Acid ◽  
Listeria Monocytogenes ◽  
Pathogenic Bacteria ◽  
Room Temperature ◽  
Storage Temperature ◽  
Chicken Meat ◽  
Coconut Water ◽  
Banana Peel ◽  
Cold Temperatures

Providing natural preservatives to increase the shelf life of chicken meat is a challenge, since the microbial contaminants problem has been a concern among many actors: government, business, consumers, and health practitioners. Acetic acid (known as vinegar) has properties as an antimicrobial, due to its ability to lower the pH and causing instability in the cell membrane of pathogenic bacteria. This paper aimed to assess the manufacture of vinegar from banana peel and coconut water as potentially natural preservative, and its application to determine the effect of microbial growth inhibition of Listeria monocytogenes in chicken meat. The study was designed using a randomized factorial design with 2 factors: 1. Types of vinegar (banana peel, coconut water, commercial acetic acid and commercial lactic acid) and 2. Storage temperature (room temperature and refrigerated temperature 5-7° C), each were repeated three times. Chicken meat that has been treated with acid soaking then stored at room temperature and cold temperatures. The results showed that banana peel and coconut water vinegar inhibit the growth of testing bacteria L. monocytogenes in chicken meat stored at room temperature more effective (5-6 log CFU/g) than the commercial acetic acid and commercial lactic acid (7-8 log CFU/g), for 24 hours storage. In chicken meat stored in cold temperatures, banana peel and coconut water vinegar had almost the same capabilities with commercial acetic acid (5.34 log CFU/g) on storage for 12 days. The most potential vinegar to be used in refrigerated temperature was banana peel vinegar.

scholarly journals Pengaruh Suhu Penyimpanan terhadap Kerusakan Antioksidan Ekstrak Daun Asam (Tamarindusindica L)

2020 ◽  
Vol 8 (4) ◽  
pp. 544
Author(s):  
Indah Dewanti Wulansari ◽  
Bambang Admadi ◽  
Sri Mulyani
Keyword(s):  
Vitamin C ◽  
Antioxidant Capacity ◽  
Leaf Extract ◽  
Room Temperature ◽  
Storage Temperature ◽  
Freezing Temperature ◽  
Cold Temperature ◽  
Total Phenolic ◽  
Tamarindus Indica ◽  
Cold Temperatures

The purpose of this study was to determine the tendency of tamarind (Tamarindus indica L.) leaf extract damage at different temperatures during storage and to determine the storage temperature that can sustain the antioxidant damage of tamarind leaf extract (Tamarindus indica L.) during storage time. Tamarind leaf extract was stored for 4 (four) weeks at 3 (three) different temperature conditions (room temperature / 27 ± 2?C; cold temperature / 5 ± 2?C; and freezing temperature / -10 ± 2?C) and analyzed for total phenolic, antioxidant capacity. and weekly levels of vitamin C. The data obtained were tabulated and analyzed descriptively. The results of this study indicate that at room temperature (27 ± 2)?C, cold temperature (5 ± 2)?C, and freezing temperature (-10 ± 2)?C, total phenolic, vitamin C levels, and antioxidant capacity have a tendency to decrease during storage. four weeks. The highest decrease in total phenolic occurred at room temperature (27 ± 2)?C, the highest decrease in vitamin C levels and antioxidant capacity occurred at freezing temperatures (-10 ± 2)?C. Storage with cold temperatures (5 ± 2)?C can retain the antioxidant content of tamarind leaf extract for four weeks and is the best storage condition. Keywords : Storage temperature, tamarind leaves extract, totalphenolicc, antioxidant, vitamin C

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