Feather Keratin Hydrolysis by an Aquatic Bacterium Meiothermus I40 from Hot Spring Water

2011 ◽  
Vol 7 (2) ◽  
Author(s):  
Jing-Iong Yang ◽  
Jen-Min Kuo ◽  
Wen-Ming Chen ◽  
Huei-Jing Ke ◽  
Yi-Ju Chou
Keyword(s):  
Yeast Extract ◽  
Incubation Temperature ◽  
Hot Spring ◽  
Fold Increase ◽  
Fermentation Conditions ◽  
Effective Factors ◽  
Extract Concentration ◽  
Initial Stage ◽  
Keratin Hydrolysis ◽  
Fermentation Parameters

After undergoing keratinase digestion, feather wastes could have a great potential as a source of proteins and amino acids for many applications. In this study, the fermentation conditions of feather-degrading Meiothermus sp. strain I40 (I40) were optimized to enhance the biodegradation of chicken feather to hydrolysates. Initially, the factors essential for I40 keratinase production in submerged fermentation were screened, whereas response surface methodology (RSM) was then employed to evaluate the interactions among the effective factors. At first stage, eight fermentation parameters were screened using a Plackett-Burman (PB) design. Four effective factors identified by PB screening, namely feather concentration, tryptone concentration, yeast extract concentration, and incubation temperature, were further investigated their effects on keratinase production by RSM using central composite design (CCD). The I40 fermentation conditions for maximal keratinase activity were as follows: tryptone concentration 0.16 percent (w/v), yeast extract concentration 0.27 percent (w/v), feather concentration 0.08 percent (w/v), and incubation temperature at 51.7°C for 72 hr under 120 rev/min shaking. Compared to the initial stage, a 13.3-fold increase in keratinase activity was achieved when I40 incubated in the optimized conditions.

scholarly journals Production of β-Glucosidase from a Newly IsolatedAspergillusSpecies Using Response Surface Methodology

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Pilanee Vaithanomsat ◽  
Molnapat Songpim ◽  
Taweesiri Malapant ◽  
Akihiko Kosugi ◽  
Warunee Thanapase ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ammonium Sulfate ◽  
Yeast Extract ◽  
Culture Medium ◽  
Specific Activity ◽  
Ph Value ◽  
Sulfate Concentration ◽  
Extract Concentration ◽  
Fermentation Parameters

A newly isolated fungusAspergillus nigerSOI017 was shown to be a good producer of β-glucosidase from all isolated fungal strains. Fermentation condition (pH, cellobiose concentration, yeast extract concentration, and ammonium sulfate concentration) was optimized for producing the enzyme in shake flask cultures. Response surface methodology was used to investigate the effects of 4 fermentation parameters (yeast extract concentration, cellobiose concentration, ammonium sulfate concentration, and pH) on β-glucosidase enzyme production. Production of β-glucosidase was most sensitive to the culture medium, especially the nitrogen source yeast extract. The optimized medium for producing maximum β-glucosidase specific activity consisted of 0.275% yeast extract, 1.125% cellobiose, and 2.6% ammonium sulfate at a pH value of 3.

scholarly journals Design and optimization of hydrogen production from hydrothermally pretreated sugarcane bagasse using response surface methodology

2017 ◽  
Vol 76 (1) ◽  
pp. 95-105 ◽  
Author(s):  
Lais Américo Soares ◽  
Juliana Kawanishi Braga ◽  
Fabrício Motteran ◽  
Isabel Kimiko Sakamoto ◽  
Edson Luiz Silva ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Hydrogen Production ◽  
Response Surface ◽  
Sugarcane Bagasse ◽  
Yeast Extract ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Incubation Temperature ◽  
P Value ◽  
Design And Optimization ◽  
Extract Concentration

Hydrogen production from hydrothermally pretreated (200 °C for 10 min at 16 bar) sugarcane bagasse was analyzed using response surface methodology. The yeast extract concentration and the temperature had a significant influence for hydrogen production (p-value 0.027 and 0.009, respectively). Maximum hydrogen production (17.7 mmol/L) was observed with 3 g/L yeast extract at 60 °C (C10). In this conditions were produced acetic acid (50.44 mg/L), butyric acid (209.71 mg/L), ethanol (38.4 mg/L), and methane (6.27 mmol/L). Lower hydrogen productions (3.5 mmol/L and 3.9 mmol/L) were observed under the conditions C7 (2 g/L of yeast extract, 35.8 °C) and C9 (1 g/L of yeast extract, 40 °C), respectively. The low yeast extract concentration and low temperature caused a negative effect on the hydrogen production. By means of denaturing gradient gel electrophoresis 20% of similarity was observed between the archaeal population of mesophilic (35 and 40 °C) and thermophilic (50, 60 and 64 °C) reactors. Likewise, similarity of 22% was noted between the bacterial population for the reactors with the lowest hydrogen production (3.5 mmol/L), at 35.8 °C and with the highest hydrogen production (17.7 mmol/L) at 60 °C demonstrating that microbial population modification was a function of incubation temperature variation.

scholarly journals Use of Wheat Straw for Value-Added Product Xylanase by Penicillium chrysogenum Strain A3 DSM105774

2021 ◽  
Vol 7 (9) ◽  
pp. 696
Author(s):  
Amira A. Matrawy ◽  
Ahmed I. Khalil ◽  
Heba S. Marey ◽  
Amira M. Embaby
Keyword(s):  
Wheat Straw ◽  
Yeast Extract ◽  
Penicillium Chrysogenum ◽  
Incubation Temperature ◽  
Value Added ◽  
Production Medium ◽  
Xylanase Production ◽  
Extract Concentration ◽  
Enhanced Production ◽  
Initial Ph

The present work highlights the valorization of the bulky recalcitrant lignocellulose byproduct wheat straw (WS) for the enhanced production of value-added xylanase by the locally sourced novel Penicillium chrysogenum strain A3 DSM105774 for the first time. The optimized production of xylanase by submerged state of fermentation of WS was achieved using a three-step statistical and sequential approach: one factor at a time (OFAT), Plackett–Burman design (PBD), and Box Behnken design (BBD). Incubation temperature (30 °C), WS, and ammonium sulphate were the key determinants prompting xylanase production; inferred from OFAT. The WS concentration (%(w/v)), yeast extract concentration (%(w/v)), and initial pH of the production medium imposed significant effects (p ≤ 0.05) on the produced xylanase, realized from PBD. The predicted levels of WS concentration, initial pH of the production medium, and yeast extract concentration provoking the ultimate xylanase levels (53.7 U/mL) with an 8.95-fold enhancement, localized by the estimated ridge of the steepest ascent of the ridge analysis path, were 3.8% (w/v), 5.1, and 0.098% (w/v), respectively; 94.7% lab validation. The current data underpin the up-scaling of xylanase production using this eco-friendly, cheap, and robust methodology for the valorization of WS into the value-added product xylanase.

Optimization of the Submerged Fermentation Conditions of Ganoderma Lucidum with High Triterpenoids Production by Response Surface Analysis

2012 ◽  
Vol 621 ◽  
pp. 259-262
Author(s):  
Mei Lin Cui ◽  
Guo Qing He
Keyword(s):  
Regression Model ◽  
Central Composite Design ◽  
Yeast Extract ◽  
Submerged Fermentation ◽  
Ganoderma Lucidum ◽  
Fermentation Conditions ◽  
Independent Variables ◽  
Extract Concentration ◽  
Set Up ◽  
Central Composite

In this paper, yeast extract concentration, incubation days and inoculation was made as independent variables, the production of intracellular triterpenoids, intracellular polysaccharides and mycelia biomass were made as response values, we set up central composite design of three factors and three levels. Through the analysis of the regression model, we could see that when yeast extract concentration was 1.91%, the inoculation was 14.99% and the incubation days was 4.24d, the production of intracellular triterpenoids, intracellular polysaccharides and mycelia biomass was 100.654 mg/100ml, 58.5968 mg/100ml, 2.39258 g/100ml, respectively.

scholarly journals Artificial Neural Networks and Response Surface Methodology Approach for Optimization of an Eco-Friendly and Detergent-Stable Lipase Production from Actinomadura Keratinilytica Strain Cpt29

2021 ◽  
Vol 68 (3) ◽  
pp. 575-586
Author(s):  
Noura Semache ◽  
Fatiha Benamia ◽  
Bilal Kerouaz ◽  
Inès Belhaj ◽  
Selma Bounour ◽  
...  
Keyword(s):  
Incubation Temperature ◽  
Tween 80 ◽  
Fold Increase ◽  
Data Fitting ◽  
Lipase Production ◽  
Potential Candidate ◽  
Cultivation Time ◽  
North East ◽  
Lipase A ◽  
Commercial Laundry

This work mainly focused on the production of an efficient, economical, and eco-friendly lipase (AKL29) from Actinomadura keratinilytica strain Cpt29 isolated from poultry compost in north east of Algeria, for use in detergent industries. AKL29 shows a significant lipase activity (45 U/mL) towards hydrolyzed triacylglycerols, indicating that it is a true lipase. For maximum lipase production the modeling and optimization of potential culture parameters such as incubation temperature, cultivation time, and Tween 80 (v/v) were built using RSM and ANN approaches. The results show that both the two models provided good quality predictions, yet the ANN showed a clear superiority over RSM for both data fitting and estimation capabilities. A 4.1-fold increase in lipase production was recorded under the following optimal condition: incubation temperature (37.9 °C), cultivation time (111 h), and Tween 80 (3.27%, v/v). Furthermore, the partially purified lipase showed good stability, high compatibility, and significant wash performance with various commercial laundry detergents, making this novel lipase a promising potential candidate for detergent industries.

Kinetics of Glycerol Biotransformation to Dihydroxyacetone by Immobilized Gluconobacter oxydans and Effect of Reaction Conditions

2007 ◽  
Vol 72 (9) ◽  
pp. 1269-1283 ◽  
Author(s):  
Jiří Raška ◽  
František Skopal ◽  
Karel Komers ◽  
Jaroslav Machek
Keyword(s):  
Yeast Extract ◽  
Batch Reactor ◽  
Gluconobacter Oxydans ◽  
Poly Vinyl Alcohol ◽  
Glycerol Concentration ◽  
Reaction Conditions ◽  
Extract Concentration ◽  
Autocatalytic Model ◽  
Kinetics Of ◽  
Reaction Schemes

Biotransformation of glycerol to 1,3-dihydroxyacetone was carried out in an isothermal isochoric batch reactor with Gluconobacter oxydans immobilized in poly(vinyl alcohol) gel capsules. The reaction course was described with a three-step kinetic model. Two reaction schemes were proposed and compared with 8 kinetic experiments at 25 °C. The experimental dependences of glycerol and dihydroxyacetone concentrations on reaction time were simulated very well by the autocatalytic model. The effects of reaction temperature and initial concentrations of yeast extract and glycerol were studied. Temperature 25-30 °C, initial yeast extract concentration 2-4 g l-1 and initial glycerol concentration 20-50 g l-1 were found as optimal. The determined rate constants can be used to advantage for industrial production of dihydroxyacetone from glycerol.

scholarly journals Moringa oleifera Seeds Extract Activity on Enteropathogenic Escherichia coli and Aeromonas hydrophyla Cells in Aquatic Microcosm

2019 ◽  
Vol 7 (2) ◽  
pp. 13 ◽  
Author(s):  
Claire Stephane Metsopkeng ◽  
Chretien Lontsi Djimeli ◽  
Olive Vivien Noah Ewoti ◽  
Lucienne Marlyse Moungang ◽  
Paul Alain Nana ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Moringa Oleifera ◽  
Potable Water ◽  
Incubation Temperature ◽  
Enteropathogenic Escherichia Coli ◽  
Incubation Condition ◽  
E Coli ◽  
Extract Concentration ◽  
Cell Incubation ◽  
Potential Exploitation

This study aimed to evaluate in microcosm condition, the survival of Aeromonas hydrophila and Enteropathogenic Escherichia coli (EPEC), in the presence of M. oleifera aqueous seeds extract at concentrations varying from 1 to 40 g/L, and under 4 °C and 23 °C incubation temperature. It has been noted that cell abundances decrease gradually with the increasing in the seeds extract concentration. However, a marked cells regrowth was sometimes noted. In monospecies cell incubation condition, under 4 °C, the EPEC cells inhibition percentages (CIP) values varied from 52.12 to 99.84%. Those of A. hydrophila varied from 13.2 to 96%. The lowest CIPs were noted at the extract concentration 1g/L for EPEC and A. hydrophila. The highest CIP value was registered at 10 and 40 g/L for EPEC and at 15 g/L for A. hydrophila. Under 23 °C incubation, the EPEC CIPs values varied from 74.04 to 99.9% and those of A. hydrophila varied from 21.2 to 97.8%. For E. coli, the lowest and the highest CIP were recorded at the extract concentration 1g/L and 30 g/L, respectively. In bispecies cells incubation condition, the CIPs were relatively different. These results show the potential exploitation of M. oleifera extracts in the microbiological treatment of potable water.

Composition of Lipopolysaccharides from Various Strains of Rhodomicrobium vannielii

1986 ◽  
Vol 41 (1-2) ◽  
pp. 202-205 ◽  
Author(s):  
Otto Holst ◽  
Jürgen Weckesser ◽  
Baldur Rieth ◽  
Crawford S. Dow
Keyword(s):  
Fatty Acids ◽  
Chemical Composition ◽  
Yeast Extract ◽  
Growth Temperature ◽  
Simple Cycle ◽  
Growth Forms ◽  
Extract Concentration ◽  
Dependent Growth ◽  
Rhodomicrobium Vannielii

Abstract The compositions of lipopolysaccharides from the photoheterotrophic budding Rhodomi­crobium vannielii strains DSM 162, Rm5, E3 and 2/1 are reported. Common constituents of these lipopolysaccharides are glucose, mannose, glucosamine, glucuronic and galacturonic acids, 2-keto-3-deoxyoctonate (KDO) and the fatty acids 3-OH-C16:0, 3-OH-C14:0, C14:0, Δ14-C22:1, aside from strain specific differences. Two different, medium-dependent growth forms of strain DSM 162 are described. The vitamin/ yeast extract-concentration in the medium and/or the growth temperature were found as factors triggering the different growth forms. Lipopolysaccharides of the two growth forms had only quantitative differences in composition. Lipopolysaccharide from swarmer cells of strain Rm5 showed a chemical composition comparable to that of chain cells and from simple cycle cells of the same strain.

Cultural conditions optimization for production of β-galactosidase from Bacillus licheniformis ATCC 12759 under solid-state fermentation

2018 ◽  
Vol 43 (3) ◽  
pp. 240-247 ◽  
Author(s):  
Nurullah Akcan
Keyword(s):  
Solid State ◽  
Moisture Content ◽  
Bacillus Licheniformis ◽  
Solid State Fermentation ◽  
Incubation Temperature ◽  
Fermentation Medium ◽  
Inoculum Level ◽  
Cultural Conditions ◽  
Initial Ph ◽  
Fermentation Parameters

AbstractObjective:The aim of this work was to study the optimal cultivation conditions for β-galactosidase production byBacillus licheniformisATCC 12759.Materials and methods:The screening of β-galactosidase production fromB. licheniformisATCC 12759 was performed by solid state fermentation method on media rich with rice bran (RB). Different factors were tested for the optimization of β-galactosidase production.Results:Certain fermentation parameters involving incubation time, incubation temperature, inoculum level, moisture content, initial pH, agitation speed, size of fermentation medium and optimum temperature of β-galactosidase activity were studied separately. Maximal amount of β-galactosidase production was obtained when solid-state fermentation (SSF) was carried out using RB, having inoculum level 35%, moisture content of 20%, initial pH 7.5 at 37°C for 48 h.Conclusion:Results indicated that optimal fermentation conditions play a key role in the maximum production of β-galactosidase fromB. licheniformisATCC 12759. This study shows the potential of the studied enzymes to be promoting candidates for the degradation of lactose and production of important bioproducts.

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