Quantitative Three-dimensional High Definition Microvessel Imaging for characterization breast masses v1

2021 ◽  
Author(s):  
Juanjuan Gu# ◽  
Redouane Ternifi# ◽  
Soroush Sabeti ◽  
Nicholas B Larson ◽  
Jodi M Carter ◽  
...  
Keyword(s):  
Malignant Tumors ◽  
Imaging Technique ◽  
Imaging Techniques ◽  
Morphological Features ◽  
Frame Rate ◽  
Imaging Method ◽  
High Definition ◽  
Breast Masses ◽  
Vessel Morphology ◽  
Novel Processing

Breast cancer, a major cause of morbidity and mortality in women, is highly dependent on angiogenesis for its growth and distant metastasis. Furthermore, new blood vessels in malignant tumors are structurally abnormal and different from benign. Imaging techniques that provide information regarding tumor microvasculature structures could aid cancer detection. This protocol is to advance the development and evaluate the performance of a quantitative 3D microvessel imaging technique to provide quantitative information of morphological features of tumor microvessel as new biomarker for differentiation of malignant and benign breast masses. Our team has developed a new 2D contrast-free ultrasound (US) microvessel imaging technique based on novel processing procedures for revealing and enhancing submillimeter size tumor microvessels and complemented this technique with novel quantification tools to quantify vessel morphology. The results of quantitative 2D-HDMI for differentiation of malignant and benign breast masses are promising; however, the 2D imaging method overlooks some important 3-dimensional (3D) morphological features, such as the connectivity of the blood microvessels, leading to under- or overestimation of these parameters. Here, we propose to advance the development of a new quantitative 3D High-Definition Microvascular Imaging (q3D-HDMI) to provide complementary diagnostic information to the conventional US. The new technique, q3D-HDMI, uses high frame rate ultrasound imaging and is based on novel processing and quantification procedures for 3D imaging to reveal and quantify microvessel morphology in tumor volume. In this protocol we evaluate the diagnostic performance of q3D-HDMI for characterization of breast masses in a population of pre-biopsy patients; correlate the results with pathology as the gold standard.We also compare q3D-HDMI and q2D-HDMI in differentiating malignant from benign breast lesions

Structural and functional imaging of large and opaque plant specimens

2019 ◽  
Vol 70 (14) ◽  
pp. 3659-3678 ◽  
Author(s):  
Linnea Hesse ◽  
Katharina Bunk ◽  
Jochen Leupold ◽  
Thomas Speck ◽  
Tom Masselter
Keyword(s):  
Imaging Technique ◽  
Imaging Techniques ◽  
Complex Geometries ◽  
Imaging Method ◽  
Resonance Imaging ◽  
Micro Computed Tomography ◽  
Thin Sections ◽  
Magnetic Resonance Imaging Mri ◽  
The Right ◽  
Ct And Mri

AbstractThree- and four-dimensional imaging techniques are a prerequisite for spatially resolving the form–structure–function relationships in plants. However, choosing the right imaging method is a difficult and time-consuming process as the imaging principles, advantages and limitations, as well as the appropriate fields of application first need to be compared. The present study aims to provide an overview of three imaging methods that allow for imaging opaque, large and thick (>5 mm, up to several centimeters), hierarchically organized plant samples that can have complex geometries. We compare light microscopy of serial thin sections followed by 3D reconstruction (LMTS3D) as an optical imaging technique, micro-computed tomography (µ-CT) based on ionizing radiation, and magnetic resonance imaging (MRI) which uses the natural magnetic properties of a sample for image acquisition. We discuss the most important imaging principles, advantages, and limitations, and suggest fields of application for each imaging technique (LMTS, µ-CT, and MRI) with regard to static (at a given time; 3D) and dynamic (at different time points; quasi 4D) structural and functional plant imaging.

scholarly journals OP0309 THE ROLE OF IMAGING TO GUIDE INTERVENTIONAL PROCEDURES IN RHEUMATIC AND MUSCULOSKELETAL DISEASES: A SYSTEMATIC LITERATURE REVIEW TO INFORM EULAR RECOMMENDATIONS

2021 ◽  
Vol 80 (Suppl 1) ◽  
pp. 189.2-190
Author(s):  
P. Bosch ◽  
F. Carubbi ◽  
C. A. Scirè ◽  
X. Baraliakos ◽  
L. Falzon ◽  
...  
Keyword(s):  
Retrospective Studies ◽  
Musculoskeletal Diseases ◽  
Imaging Technique ◽  
Imaging Techniques ◽  
Axial Skeleton ◽  
Cochrane Library ◽  
Imaging Method ◽  
Imaging Guidance ◽  
Targeted Interventions

Background:Imaging guidance gives the opportunity to visualize a needle or other instrument when performing interventions on musculoskeletal sites in patients with rheumatic and musculoskeletal diseases (RMDs). Studies assessing the role of imaging guided procedures have been performed, however, no consensus has been made yet about when and which imaging techniques to use for these interventions.Objectives:To inform a EULAR taskforce on the current literature on different imaging techniques, procedures and materials to guide interventions on musculoskeletal sites in RMD patients.Methods:Prospective and retrospective studies published in English and comparing either palpation or imaging guided interventions in patients with RMDs were included. MEDLINE, EMBASE, the Cochrane Library and Epistemonikos were searched through October 2021. Risk of bias (RoB) was assessed using the Cochrane RoB tool for randomized trials version 2 (ROB2), the RoB tool for Non-Randomized Studies of Interventions (ROBINS-I) and the appraisal tool for cross-sectional studies (AXIS).Results:Sixty-six studies, with moderate to high RoB, consisting of 49 randomized controlled trials, three prospective cohort studies and 14 retrospective studies were included.Fifty-one studies compared one imaging technique against either another imaging technique, or palpation-guided interventions. Most of the studies were on peripheral joints (47/51), while data on joints of the axial skeleton were scare and heterogeneous (4/51). For peripheral joints, ultrasound (US) was the most studied imaging technique (49/51), followed by fluoroscopy (10/51). Results revealed a higher accuracy and safety (including procedural pain) of US or fluoroscopy compared to palpation- guided interventions. Data on other imaging techniques was scarce (computed tomography: n=3, arthroscopy: n=1) and the results heterogeneous. Results of studies comparing different imaging techniques (12/51) did not favor one imaging method over another.Three studies comparing different materials used for imaging guided interventions were found (e.g. automatic vs manual syringes), showing little evidence for one material being superior to another one.Fifteen studies were found comparing different imaging guided procedures (e.g. intraarticular vs periarticular injections). Overall, studies indicated an advantage of targeted vs. not targeted interventions (intraarticular vs periarticular or intraepineurial vs extraepineurial injections) concerning pain levels, while the comparison of different puncture sites to inject (e.g. ulnar vs midline carpal tunnel injection) was inconclusive.Conclusion:Imaging guidance, especially US, performs favorably for interventions at the peripheral joints compared to palpation-guided interventions, concerning accuracy and safety. Data for the axial skeleton are scarce. Imaging guided targeted interventions may lead to better outcomes than non-targeted interventions.Disclosure of Interests:None declared.

Cardiovascular Imaging for Guiding Interventional Therapy in Structural Heart Diseases

2020 ◽  
Vol 16 (2) ◽  
pp. 111-122
Author(s):  
Nora Rat ◽  
Iolanda Muntean ◽  
Diana Opincariu ◽  
Liliana Gozar ◽  
Rodica Togănel ◽  
...  
Keyword(s):  
Heart Diseases ◽  
Interventional Procedure ◽  
Imaging Techniques ◽  
Ductus Arteriosus ◽  
Three Dimensional ◽  
High Specificity ◽  
Interventional Therapy ◽  
Structural Defects ◽  
Imaging Method ◽  
Three Dimensional Echocardiography

Development of interventional methods has revolutionized the treatment of structural cardiac diseases. Given the complexity of structural interventions and the anatomical variability of various structural defects, novel imaging techniques have been implemented in the current clinical practice for guiding the interventional procedure and for selection of the device to be used. Three– dimensional echocardiography is the most used imaging method that has improved the threedimensional assessment of cardiac structures, and it has considerably reduced the cost of complications derived from malalignment of interventional devices. Assessment of cardiac structures with the use of angiography holds the advantage of providing images in real time, but it does not allow an anatomical description. Transesophageal Echocardiography (TEE) and intracardiac ultrasonography play major roles in guiding Atrial Septal Defect (ASD) or Patent Foramen Ovale (PFO) closure and device follow-up, while TEE is the procedure of choice to assess the flow in the Left Atrial Appendage (LAA) and the embolic risk associated with a decreased flow. On the other hand, contrast CT and MRI have high specificity for providing a detailed description of structure, but cannot assess the flow through the shunt or the valvular mobility. This review aims to present the role of modern imaging techniques in pre-procedural assessment and intraprocedural guiding of structural percutaneous interventions performed to close an ASD, a PFO, an LAA or a patent ductus arteriosus.

scholarly journals Design and Synthesis of Luminescent Lanthanide-Based Bimodal Nanoprobes for Dual Magnetic Resonance (MR) and Optical Imaging

Nanomaterials ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 354
Author(s):  
Walid Mnasri ◽  
Mahsa Parvizian ◽  
Souad Ammar-Merah
Keyword(s):  
Magnetic Resonance ◽  
Optical Imaging ◽  
Imaging Technique ◽  
Imaging Techniques ◽  
Design And Synthesis ◽  
Bimodal Imaging ◽  
Successive Image ◽  
Complete Set ◽  
Magnetic Resonance Imaging Mri ◽  
Physical And Chemical

Current biomedical imaging techniques are crucial for the diagnosis of various diseases. Each imaging technique uses specific probes that, although each one has its own merits, do not encompass all the functionalities required for comprehensive imaging (sensitivity, non-invasiveness, etc.). Bimodal imaging methods are therefore rapidly becoming an important topic in advanced healthcare. This bimodality can be achieved by successive image acquisitions involving different and independent probes, one for each mode, with the risk of artifacts. It can be also achieved simultaneously by using a single probe combining a complete set of physical and chemical characteristics, in order to record complementary views of the same biological object at the same time. In this scenario, and focusing on bimodal magnetic resonance imaging (MRI) and optical imaging (OI), probes can be engineered by the attachment, more or less covalently, of a contrast agent (CA) to an organic or inorganic dye, or by designing single objects containing both the optical emitter and MRI-active dipole. If in the first type of system, there is frequent concern that at some point the dye may dissociate from the magnetic dipole, it may not in the second type. This review aims to present a summary of current activity relating to this kind of dual probes, with a special emphasis on lanthanide-based luminescent nano-objects.

Full-field X-ray diffraction microscopy using polymeric compound refractive lenses

2014 ◽  
Vol 47 (6) ◽  
pp. 1882-1888 ◽  
Author(s):  
J. Hilhorst ◽  
F. Marschall ◽  
T. N. Tran Thi ◽  
A. Last ◽  
T. U. Schülli
Keyword(s):  
Imaging Techniques ◽  
Light And Electron Microscopy ◽  
Added Value ◽  
Acquisition Time ◽  
Imaging Method ◽  
X Ray Diffraction ◽  
Polymeric Compound ◽  
Full Field ◽  
X Ray ◽  
Diffraction Imaging

Diffraction imaging is the science of imaging samples under diffraction conditions. Diffraction imaging techniques are well established in visible light and electron microscopy, and have also been widely employed in X-ray science in the form of X-ray topography. Over the past two decades, interest in X-ray diffraction imaging has taken flight and resulted in a wide variety of methods. This article discusses a new full-field imaging method, which uses polymer compound refractive lenses as a microscope objective to capture a diffracted X-ray beam coming from a large illuminated area on a sample. This produces an image of the diffracting parts of the sample on a camera. It is shown that this technique has added value in the field, owing to its high imaging speed, while being competitive in resolution and level of detail of obtained information. Using a model sample, it is shown that lattice tilts and strain in single crystals can be resolved simultaneously down to 10−3° and Δa/a= 10−5, respectively, with submicrometre resolution over an area of 100 × 100 µm and a total image acquisition time of less than 60 s.

scholarly journals Tracking Transplanted Stem Cells Using Magnetic Resonance Imaging and the Nanoparticle Labeling Method in Urology

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Jae Heon Kim ◽  
Hong J. Lee ◽  
Yun Seob Song
Keyword(s):  
Magnetic Resonance Imaging ◽  
Stem Cells ◽  
Molecular Imaging ◽  
Magnetic Resonance ◽  
Imaging Modality ◽  
Imaging Techniques ◽  
Image Resolution ◽  
Imaging Method ◽  
Resonance Imaging

A reliablein vivoimaging method to localize transplanted cells and monitor their viability would enable a systematic investigation of cell therapy. Most stem cell transplantation studies have used immunohistological staining, which does not provide information about the migration of transplanted cellsin vivoin the same host. Molecular imaging visualizes targeted cells in a living host, which enables determining the biological processes occurring in transplanted stem cells. Molecular imaging with labeled nanoparticles provides the opportunity to monitor transplanted cells noninvasively without sacrifice and to repeatedly evaluate them. Among several molecular imaging techniques, magnetic resonance imaging (MRI) provides high resolution and sensitivity of transplanted cells. MRI is a powerful noninvasive imaging modality with excellent image resolution for studying cellular dynamics. Several types of nanoparticles including superparamagnetic iron oxide nanoparticles and magnetic nanoparticles have been used to magnetically label stem cells and monitor viability by MRI in the urologic field. This review focuses on the current role and limitations of MRI with labeled nanoparticles for tracking transplanted stem cells in urology.

scholarly journals Respiratory-gated KES imaging of a rat model of acute lung injury at the Canadian Light Source

2017 ◽  
Vol 24 (3) ◽  
pp. 679-685 ◽  
Author(s):  
P. Deman ◽  
S. Tan ◽  
G. Belev ◽  
N. Samadi ◽  
M. Martinson ◽  
...  
Keyword(s):  
Lung Injury ◽  
Contrast Agent ◽  
Light Source ◽  
Imaging Techniques ◽  
Image Acquisition ◽  
Brown Norway ◽  
Pulmonary Vasculature ◽  
Imaging Method ◽  
Imaging Protocol ◽  
Iodinated Contrast

In this study, contrast-enhanced X-ray tomographic imaging for monitoring and quantifying respiratory disease in preclinical rodent models is proposed. A K-edge imaging method has been developed at the Canadian Light Source to very accurately obtain measurements of the concentration of iodinated contrast agent in the pulmonary vasculature and inhaled xenon in the airspaces of rats. To compare the iodine and xenon concentration maps, a scout projection image was acquired to define the region of interest within the thorax for imaging and to ensure the same locations were imaged in each K-edge subtraction (KES) acquisition. A method for triggering image acquisition based on the real-time measurements of respiration was also developed to obtain images during end expiration when the lungs are stationary, in contrast to other previously published studies that alter the respiration to accommodate the image acquisition. In this study, images were obtained in mechanically ventilated animals using physiological parameters at the iodine K-edge in vivo and at the xenon K-edge post mortem (but still under mechanical ventilation). The imaging techniques were performed in healthy Brown Norway rats and in age-matched littermates that had an induced lung injury to demonstrate feasibility of the imaging procedures and the ability to correlate the lung injury and the quantitative measurements of contrast agent concentrations between the two KES images. The respiratory-gated KES imaging protocol can be easily adapted to image during any respiratory phase and is feasible for imaging disease models with compromised lung function.

scholarly journals Imaging techniques in the assessment of endovascular infrarenal abdominal aortic repair

2020 ◽  
Vol 73 (1-2) ◽  
pp. 29-35
Author(s):  
Aleksandar Spasic ◽  
Viktor Till ◽  
Marijana Basta-Nikolic ◽  
Djordje Milosevic ◽  
Darka Hadnadjev-Simonji ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Computed Tomography Angiography ◽  
Imaging Techniques ◽  
First Year ◽  
Imaging Method ◽  
Diagnostic Modality ◽  
Aortic Repair ◽  
Abdominal Aortic ◽  
Enhanced Computed Tomography

Introduction. Imaging is essential in the assessment of endovascular infrarenal abdominal aortic repair results. Complications include endoleaks, graft migration, kinking and infolding, stenosis, occlusion, and secondary ruptures. Examination Modalities. Contemporary imaging strategies are based on using noninvasive imaging modalities. After endovascular infrarenal abdominal aortic repair, the standard evaluation modality is computed tomography angiography, whereas additional modalities include magnetic resonance imaging, ultrasonography, and radiography. However, although an invasive imaging method, digital subtraction angiography is still performed in some patients. Computed tomography angiography provides excellent contrast, spatial resolution, and exact measurements of structures of interest, which is essential in the follow-up. Follow-up Protocol. Currently recommended follow-up protocol in the first year is contrast- enhanced computed tomography imaging at 1 and 12 months after the procedure. Conclusion. Due to its characteristics, reproducibility and availability, computed tomography angiography remains the cornerstone diagnostic modality of post-procedural assessment in patients with endovascular infrarenal abdominal aortic repair.

scholarly journals Comparison between cystourethrography and sonourethrography in preoperative diagnostic management of patients with anterior urethral strictures

2018 ◽  
Vol 20 (4) ◽  
pp. 436
Author(s):  
Jakub Krukowski ◽  
Adam Kałużny ◽  
Jakub Kłącz ◽  
Marcin Matuszewski
Keyword(s):  
Imaging Techniques ◽  
Imaging Method ◽  
Urethral Strictures ◽  
Preoperative Diagnostic ◽  
Diagnostic Management ◽  
The Mean

Aim: To evaluate the urethral lesions and the degree of spongiofibrosis using cystourethrography (CUG) and sonourethrography (SUG) in order to propose the best imaging method for further surgical treatment.Material and methods: The study involved 66 patients with anterior urethral strictures with indication for urethroplasty. Results of CUG and SUG were compared with each other and data from surgical protocol.Results: Totally 72 strictures were detected; 47 in the bulbar part of urethra and 25 in the penile urethra. The mean length of the stenosis was 16.43 mm for CUG and 27.41 mm for SUG and 31.05 mm during surgery. The correlation levels between imaging techniques and intraoperative measurements were 0.55 (p<0.001) for CUG and 0.73 (p<0.001) for SUG. After dividing the strictures according to their location, better correlation for stenoses was obtained in penile urethra: 0.66 (p<0.001) for CUG and 0.86 (p<0.001) for SUG.Conclusions: SUG seems to be a simple and fast examination to evaluate urethral strictures. It is more accurate in comparison to CUG and gives a possibility to assess the spongiofibrosis. This information suggests that SUG can be a good complement to CUG in diagnosis of anterior urethtral strictures.

scholarly journals Political Imaging Techniques Carried Out by Baitul Muslimin Indonesia (BASUMI) in Increasing Public Political Support for PDI Perjuangan Medan City

2019 ◽  
Vol 2 (3) ◽  
pp. 164-174
Author(s):  
Sutarto Sutarto ◽  
Suwardi Lubis ◽  
Katimin Katimin
Keyword(s):  
Social Services ◽  
Social Service ◽  
Imaging Technique ◽  
Imaging Techniques ◽  
Political Support ◽  
The Political ◽  
Good Image ◽  
The People ◽  
Service Activities ◽  
Community Trust

BAMUSI is a new breakthrough for PDI Perjuangan to restore the negative stigmatization directed at PDI Perjuangan so far. BAMUSI is here to build a good image of PDI Perjuangan which has only been buzzing and narrating as a national and religious based party. BAMUSI will make a movement towards Islamic understanding that is rahmatan lil 'alamin bagi for the nation. Political imaging techniques carried out by BAMUSI in increasing the political support of the people of PDI Perjuangan Medan, namely to carry out social services: Social service activities carried out by BAMUSI Medan City is an accurate strategy in approaching and touching the hearts of Medan people in fostering community trust. Delivering Assistance: The political imaging technique carried out by BAMUSI Medan City in increasing community political support for PDI Perjuangan is by channeling aid.

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