scholarly journals Assessment of the effect of hemostatic materials on blood calcium concentration

2021 ◽  
Vol 8 (2) ◽  
pp. 43-50
Author(s):  
V. A. Lipatov ◽  
S. V. Lazarenko ◽  
D. A. Severinov
Keyword(s):  
Tranexamic Acid ◽  
Serum Calcium ◽  
Calcium Level ◽  
Calcium Concentration ◽  
Comparative Investigation ◽  
Control Group ◽  
Cellulose Derivatives ◽  
Blood Calcium ◽  
Hemostatic Agents

Purpose of the study. Assessment of the effect of hemostatic materials on concentration of calcium of blood.Materials and methods. The following groups of local hemostatic materials were studied: collagen plate (No. 2), hemostatic sponge from medical gelatin (No. 3), oxidized cellulose material (No. 4), experimental samples of hemostatic sponge developed by the team of authors, based on the sodium salt of carboxymethylcellulose with various modifications (groups No. 5-8). In the control group (No. 1), only blood of volunteer donors was used, without introducing the test materials. Evaluation of the effect of hemostatic materials on blood calcium concentration was carried out according to the described method: «Method for comparative investigation of the effectiveness of local hemostatic agents in an in vitro experiment» Russian patent No. 2709 517. According to the results of the study, median, 25 and 75 percentiles were calculated. The validity of differences between groups was determined using the non-parametric Mann-Whitney test (p<0.05).Results. In groups using various materials, such as group No. 2 (Tachocomb) by 0.24 mmol/L and group № 4 (Surgicel Fibrillar) by 0.7 mmol/L, the serum calcium level was statistically significantly lower than the control group. Significant differences of calcium level values after blood immersion of volunteer donor samples based on cellulose derivatives were revealed, namely in group No. 5 (Na-CMC + Tranexamic acid pressed) value exceeds that in group No. 4 by 0.58 mmol/l. In other cases, comparisons of calcium concentration values in groups using experimental samples (not introduced into clinical practice) with material widely used in abdominal surgery (group No. 4) were found to be greater: in group No. 6 (Na-CMC + Tranexamic acid unsaturated) by 0.61 mmol/l, in group No. 7 (Na-CMC pressed) by 0.75 mmol/l, in group No. 8 (Na-CMC unsaturated) by 0.5 mmol/l.Conclusion. It should be noted that although there are no significant differences in the groups using experimental samples of local hemostatic agents and the control group, the calcium levels in groups No. 5-6 are close to those in the groups using widely known materials such as Tachocomb (No. 2) and Gelita-Spon Standart (No. 3). Also, among the samples developed by the authors, the greatest effectiveness was identified in group No. 8 (Na-CMC, unpressed), in which the serum calcium levels (2.14 mmol/L) are lower than in groups No. 5-7.

scholarly journals Serum calcium and serum phosphate levels in transfusion dependent beta thalassemia

2018 ◽  
Vol 13 (2) ◽  
pp. 54-58
Author(s):  
Mst Ariza Sultana ◽  
Qazi Shamima Akhter
Keyword(s):  
Serum Calcium ◽  
Calcium Level ◽  
High Serum ◽  
Serum Phosphate ◽  
Phosphate Level ◽  
Beta Thalassemia ◽  
Control Group ◽  
Serum Phosphate Level ◽  
Ethical Aspect ◽  
Cross Sectional

Background: Patients with transfusion dependent beta thalassemia with severeanemia require regular blood transfusion to improve quality of life. This can lead to iron overload which might cause various complications including hypocalcaemia. Objective: To estimate the serum calcium and phosphate levels in transfusion dependent beta thalassemia patients. Methods: This cross-sectional study was conducted in the Department of Physiology, Dhaka Medical College, Dhaka from July 2016 to June 2017. After fulfilling the ethical aspect, a total number of 60 subjects were selected with the age ranging from 5 to 25 years. Among them, 40 transfusion dependent beta thalassemia patients were selected as the study group and 20 age and sex matched apparently healthy individuals were considered as control group for comparison. The study population were selected from Thalassemia foundation hospital, Dhaka. Theserum calcium and phosphate levels were estimated by autoanalyzer.. For statistical analysis, unpaired Student’s‘t’ test, Chi-square test were performed as applicable. Results: In this study, serum calcium level were significantly (p < 0.001) lower and serum phosphate level were significantly (p < 0.001) higher in transfusion dependent beta thalassemia patients as compared to healthy controls. In addition, 67.5% thalassemia patients had hypocalcemia (calcium level < 8.5 mg/dl) and 85% of thalassemia patients had hyperphosphatemia(phosphate level > 4.7mg/dl). Conclusions: This study concludes transfusion dependent beta thalassemiapatients have low calcium level and high serum phosphate level which should be monitored to avoid complications related to hypocalcaemia and hyperphosphatemia. J Bangladesh Soc Physiol. 2018, December; 13(2): 54-58

The Mechanism, Regulatory Effect of Wnt5α/Ca2+ Signaling Pathway on hMSCs Osteogenic Differentiation Under Infection Conditions

2019 ◽  
Vol 9 (12) ◽  
pp. 1706-1711
Author(s):  
Yilizati Yilihamu ◽  
Liu Yansong ◽  
Xu Mingliang ◽  
Cao Tianyong ◽  
Shi Rongjian
Keyword(s):  
Osteogenic Differentiation ◽  
Signaling Pathway ◽  
Calcium Concentration ◽  
Wnt Signaling Pathway ◽  
Quantitative Detection ◽  
Control Group ◽  
Specific Gene ◽  
Beta Catenin ◽  
Alizarin Red

Background: The paper aimed to reveal the role and possible regulatory mechanisms of Wnt5 gene and its non-canonical Beta-catenin signaling pathway in osteogenic differentiation and bone repairment of hMSCs under infection environment. Material and Methods : CCK-8 method was employed to detect the effect of SPA on the proliferation of different hMSCs cells. The histological staining of alizarin red was used to observe the differentiation of cells into osteoblasts. The semi-quantitative detection of osteoblast alkaline phosphatase (ALP) was performed. Fluo-2 assay was employed to measure the intracellular calcium concentration of different hMSCs before and after osteogenic differentiation stimulated by SPA. RT-PCR was applied to detect the specific gene fluorescence of osteoblasts differentiation. Western Blotting was employed to determine the differentiation of hMSCs into osteoblasts. Results: Different hMSCs were able to proliferate in vitro under the stimulation of 100 ng/mL SPA. CCK-8 kit was used to detect lentivirus-infected hMSCs and non-transduced hMSCs in vitro. The results showed that all the cells gradually proliferate with time and an increasing rate. The hMSCs treated with different Wnt5 genes were stained with alizarin red after osteogenic differentiation, and the activities of AMSCs ALP increased. The intracellular free calcium concentration increased with the induction time and peaked in the Wnt5 overexpression group. PCR results showed that ALP, Runx-2, Collagen I, OC, Osterix and BSP genes were all increased in contrast with the non-induction Control group. Conclusion: Under the inflammatory condition of SPA virulence factors, non-canonical Wnt signaling pathway is associated with the classical Wnt/ β-catenin signaling pathway in the process of osteogenic differentiation of hMSCs in vitro, which has a certain synergistic effect.

Effect of vitamin D and dietary calcium on parathyroid activity

1965 ◽  
Vol 209 (3) ◽  
pp. 637-642 ◽  
Author(s):  
William Y. W. Au ◽  
Lawrence G. Raisz
Keyword(s):  
Vitamin D ◽  
Serum Calcium ◽  
Calcium Intake ◽  
Calcium Concentration ◽  
Acid Uptake ◽  
Serum Calcium Concentration ◽  
Calcium Diet ◽  
High Calcium ◽  
Normal Calcium

The effects of variations in vitamin D and calcium intake on parathyroid weight and amino acid uptake were studied in vivo. D-deficient rats on low or normal calcium intake developed hypocalcemia, parathyroid enlargement, and increased parathyroid uptake of α-aminoisobutyric acid (AIB). D-deficient rats fed a high-calcium diet and D-treated rats fed a normal-calcium diet had normal serum calcium concentrations, smaller parathyroids, and lower parathyroid uptake of AIB. When serum calcium concentration of vitamin D-deficient rats was increased acutely by vitamin D treatment, dietary lactose, or injection of calcium, parathyroid uptake of AIB decreased. Low-calcium medium stimulated and high-calcium medium suppressed AIB uptake of parathyroids from vitamin D-deficient rats in vitro. Parathyroids from vitamin D-deficient rats secreted bone-resorbing material in tissue cultures. The data indicate that both size and functional activity of rat parathyroids are inversely related to serum calcium concentration, and do not depend on the presence or absence of vitamin D.

scholarly journals The Effect of Tranexamic Acid Administration on Early Endothelial Damage Following Posterior Lumbar Fusion Surgery

2021 ◽  
Vol 10 (7) ◽  
pp. 1415
Author(s):  
Hye Jin Kim ◽  
Bora Lee ◽  
Byung Ho Lee ◽  
So Yeon Kim ◽  
Byongnam Jun ◽  
...  
Keyword(s):  
Postoperative Complications ◽  
Tranexamic Acid ◽  
Lumbar Fusion ◽  
Endothelial Glycocalyx ◽  
Control Group ◽  
Fusion Surgery ◽  
Posterior Lumbar Fusion ◽  
Lumbar Fusion Surgery ◽  
Post Surgery

Tranexamic acid (TXA) protects against endothelial glycocalyx injury in vitro. We aimed to evaluate whether TXA could protect against endothelial glycocalyx degradation in patients undergoing posterior lumbar fusion surgery. Patients aged 30–80 years were enrolled. The TXA group was administered a loading dose of 10 mg/kg, followed by a 1 mg/kg/h infusion. Serum syndecan-1 and heparan sulfate concentrations, which are biomarkers of glycocalyx degradation, were measured at preoperative baseline (T0), immediately post-surgery (T1), and 2 h post-surgery (T2). Postoperative complications were assessed, including hypotension, desaturation, and acute kidney injury. Among the 121 patients who completed the study, 60 received TXA. There were no significant differences in the marker concentrations at each time point. However, the postoperative increase in syndecan-1 levels from baseline was significantly attenuated in the TXA group compared with the control group (median (interquartile range); T1 vs. T0: −1.6 (−5.3–2.6) vs. 2.2 (−0.7–4.8), p = 0.001; T2 vs. T0: 0.0 (−3.3–5.5) vs. 3.6 (−0.1–9.3), p = 0.013). Postoperative complications were significantly associated with the magnitude of the change in syndecan-1 levels (for T2 vs. T0: odds ratio: 1.08, 95% confidence interval: 1.02–1.14, p = 0.006). TXA administration was associated with reduced syndecan-1 shedding in patients undergoing posterior lumbar fusion surgery.

scholarly journals Consumption of elicited soybean sprout flour increases calcium level in serum of ovariectomized rats

2017 ◽  
Vol 36 (2) ◽  
pp. 94
Author(s):  
Siti Aminah ◽  
Suparmo Suparmo ◽  
Sri Naruki ◽  
Hastari Wuryastuty
Keyword(s):  
Serum Calcium ◽  
Calcium Level ◽  
Bone Mineralization ◽  
Serum Phosphorus ◽  
Ovariectomized Rats ◽  
Control Group ◽  
Photometric Method ◽  
Essential Components ◽  
Calcium And Phosphorus ◽  
Phosphorus Levels

<p>Background<br />The decline in estrogens could lead to an imbalance of bone formation and absorption as well as bone mineralization. Phytoestrogens are known to have the potential to maintain bone health. Calcium and phosphorus are essential components for bone mineralization. This study aimed to investigate the effect of soybean sprout flour consumption on serum calcium and phosphorus levels in ovariectomized rats.</p><p>Methods<br />A randomized post-test-only control group design involving 25 female Sprague-Dawley rats aged 2 months, which were divided into five groups: (i) normal controls on standard feed diet AIN-93 (C-N), (ii) ovariectomy controls on standard feed diet (OVX -N); (iii) ovariectomized rats on standard feed diet + ethinylestradiol (OVX -E), (iv) ovariectomized rats on standard feed diet + soybean flour (OVX -S); (v) ovariectomized rats on standard feed diet + soybean sprout flour (OVX -SS). The rats were treated for 6 weeks, then on the last day of treatment blood sampling was conducted. Analysis of calcium and phosphorus in serum was performed using the Arsenazo III photometric method. Data were analyzed using one-way Anova.</p><p>Results<br />Mean serum calcium level was highest in the OVX-SS group (12.59 mg/dL ± 1.32), while mean serum phosphorus level was highest in the C-N group (1.708 mg/dL ± 0.28). Soybean sprout flour consumption had a significant effect on serum calcium levels (p=0.019), yet it had no effect on body weight and serum phosphorus levels of the rats.</p><p>Conclusion<br />Consumption of soybean sprout flour could positively affect serum calcium levels in rats.</p>

scholarly journals To Study the Association of Total Serum Calcium Level in Assessing the Severity of Pancreatitis

2021 ◽  
pp. 781-785
Author(s):  
V. Lingesh Kumar ◽  
Saravanan Sundaram
Keyword(s):  
Acute Pancreatitis ◽  
Serum Calcium ◽  
Calcium Level ◽  
Serum Calcium Level ◽  
Tertiary Care ◽  
Total Serum ◽  
Total Calcium ◽  
Blood Calcium ◽  
Total Serum Calcium ◽  
Total Serum Calcium Level

Background: Pancreatitis is an inflammatory condition that affects the pancreas and may also affect other tissues or distant organ. In this study our aim is to see if blood calcium level can be used as a prognostic marker in determining the severity of acute pancreatitis because hypocalcaemia is linked to higher mortality and hospitalization. Method: A retrospective study was conducted in the medical records department of a tertiary care health facility. A total of 75 participants were included in the research. .demographic details of all patients were collected .Patients above 18 years who presented with of epigastric pain, pre-Diagnosed gallstones, alcoholism were included int this study. The lowest total calcium levels were collected in order to examine total calcium as a predictive factor for severity. All of these information were collected and analyzed to get the appropriate findings. Results: A total of 75 patients were included in the research. The age of patients in each severity grade did not differ significantly (p value>0.05). The gender distribution of the two groups did not differ. (P >0.05). For mild and severe acute pancreatitis, mean total calcium levels were 7.98 and 6.67, respectively, which was statistically significant (p< 0.05). Conclusion: The total serum calcium level is a useful indicator for assessing  pancreatitis.

scholarly journals The effect of fluoride on the serum level of calcium in the rat (Rattus norvegicus)

2012 ◽  
Vol 64 (4) ◽  
pp. 1585-1589
Author(s):  
M. Focak ◽  
E. Haskovic ◽  
D. Suljevic
Keyword(s):  
Serum Calcium ◽  
Rattus Norvegicus ◽  
Calcium Concentration ◽  
Gluteus Maximus ◽  
Female Rats ◽  
Male Rats ◽  
Control Group ◽  
Serum Calcium Concentration ◽  
Average Serum ◽  
Experimental Group

The effect of fluoride on the calcium level in serum was analyzed in the laboratory rat Rattus norvegicus. The control group consisted of 10, and the experimental group of 15 animals. In the experimental group, fluoride at a concentration of 3 mg/100 g body weight of rats was intramuscularly injected into the musculus gluteus maximus. The concentration of calcium was measured by the CPC method. The average serum calcium concentration was 2.46 mmol/l, with female rats having higher values of serum calcium than male rats. Fluoride caused the reduction of calcium concentration in serum (p<0.05); the reduction was significantly expressed in female rats (p<0.000).

scholarly journals A cross sectional study to evaluate serum calcium levels among pregnant women and it's association with preeclampsia and delivery outcomes at tertiary care Hospital Bikaner, Rajasthan

2021 ◽  
Vol 10 (5) ◽  
pp. 2026
Author(s):  
Laxmi Poonia ◽  
Swati Kochar ◽  
Shweta Chaudhary ◽  
Priyanka Gaur ◽  
Kirti Solanki
Keyword(s):  
Pregnant Women ◽  
Serum Calcium ◽  
Calcium Level ◽  
Serum Calcium Level ◽  
Tertiary Care ◽  
Cross Sectional Study ◽  
Control Group ◽  
Care Hospital ◽  
Sectional Study ◽  
Cross Sectional

Background: Hypertensive disorders of pregnancy, including preeclampsia complicates about 10% of pregnancies worldwide. Preeclampsia is one of the major cause of maternal and perinatal morbidity and mortality worldwide. According to the National Health Portal of India, the incidence of preeclampsia is reported to be about 8-10% among pregnant women. The present study was aimed to compare mean serum calcium levels in preeclamptic and normotensive pregnant women at third trimester of gestation and assess relationship between maternal serum calcium levels, severity of disease and overall maternal and perinatal outcome.Methods: This was a cross sectional study conducted on 200 females divided into two groups (i.e. Study and Control Group) from 1st November 2019 to 31st October 2020. Informed consent was obtained for subjecting. Detailed history and clinical examination were performed. Serum calcium level was measured in both groups by spectrophotometric method.Results: Mean serum calcium level in study group (preeclamptic) was 7.84+0.74mg/dl while in control group (normotensive) mean serum calcium level was 9.68+0.97mg/dl. Serum calcium level was significantly lower in preeclamptic women than normotensive pregnant women (p<0.001).Serum calcium showed significant negative correlation with systolic and diastolic blood pressure.Conclusions: This study concludes that hypocalcaemia may have a role in aetiology of preeclampsia and adverse maternal and perinatal outcome. Thus intake of calcium supplements may help in reduction of incidence of preeclampsia especially in a population of a developing country. 

The influence of vitamin D intake on serum calcium in tuberculosis

1981 ◽  
Vol 97 (1) ◽  
pp. 114-117 ◽  
Author(s):  
Grant Gwinup ◽  
Guy Randazzo ◽  
Alan Elias
Keyword(s):  
Vitamin D ◽  
Serum Calcium ◽  
Calcium Concentration ◽  
Dietary Vitamin ◽  
Controlled Study ◽  
Control Group ◽  
Serum Calcium Concentration ◽  
Significant Difference ◽  
Supplemental Vitamin ◽  
Prospective Controlled Study

Abstract. We report the first prospective controlled study designed to determine the effect of vitamin D ingestion on serum calcium concentration in patients with tuberculosis. Every patient admitted to the tuberculosis ward over a 6 month period, who was free of any condition which might influence serum calcium concentration, was randomly assigned to one of two groups. The diet of the first group was substituted with ergocalciferol 5000 units daily. The diet of the second group was not supplemented. In addition, the second group was randomly subdivided into two subgroups. The first subgroup received a diet unrestricted in vitamin D. The second subgroup received a diet containing less than 50 units of vitamin D. Serum calcium was determined at weekly intervals. In contradistinction to the results of a previously reported retrospective study, there was no significant difference between the group receiving supplemental vitamin D and the control group at any time during the entire period of study. Furthermore, there was no significant difference between the subgroup of patients receiving normal dietary vitamin D and the sungroup maintained on the diet restricted in vitamin D.

scholarly journals Effect of prostaglandin F2α administration during the first postpartum hours on the serum calcium concentration of dairy cows

2014 ◽  
Vol 1 (2) ◽  
Author(s):  
Eligio Gabriel Salgado-Hernández ◽  
Jan Bouda ◽  
Adolfo Aparicio-Cecilio-A ◽  
Jaroslav Doubek ◽  
Francisco Hernany Velásquez-Forero
Keyword(s):  
Dairy Cows ◽  
Serum Calcium ◽  
Repeated Measures ◽  
Calcium Concentration ◽  
Prostaglandin F2α ◽  
Control Group ◽  
Synthetic Analog ◽  
Serum Calcium Concentration ◽  
Repeated Measures Design ◽  
Group 2

Subclinical hypocalcemia occurs frequently in dairy cows and predisposes them to reproductive pathologies. Prostaglandin F2α (PGF2α) improves uterine health and increases the level of serum calcitriol in other species. The aim of this study was to evaluate the effect of sodium cloprostenol on serum calcium, metabolites of vitamin D and reproductive variables. Eighty multiparous Holstein cows were selected and randomized into two groups. The cows in Group 1 (n = 38) were treated with 500 µg of I M sodium cloprostenol, a synthetic analog of PGF2α, at 1 and 48 h postpartum. Group 2 was the control group (n = 42). Blood samples were taken from 10 animals per group at 1, 3, 12, 24, 48 h and 7 d postcalving. The incidence of retained placenta, metritis and endometritis and the number of open days were recorded. The concentrations of Ca, P, Mg, calcidiol and calcitriol in the serum were evaluated using an analysis of variance for repeated measures design. The serum Ca concentration was increased up to normalized at day 7 in the PGF2α group compared with the level in the control group. PGF2α treatment increased the serum calcium concentration and reduced the number of open days, and thus, could be useful in preventing subclinical hypocalcemia and postpartum reproductive problems.

Sign in / Sign up

Export Citation Format

Share Document