scholarly journals CARLAVIRUS IN LILY COLLECTION OF GRISHKO' NATIONAL BOTANICAL GARDEN

Author(s):  
G. Korotyeyeva ◽  
G. Petrova ◽  
L. Kykot ◽  

Screening of Lilium plants on virus diseases in the collections of M.M. Grishko' National Botanical Garden have been conducted. Basing on serological, biological and morphological properties, we suggest that filamentous virus is related to Lily symptomless virus. Another virus is not completely identified, but symptoms induced on indicator plants suggested that it was Tomato aspermy virus.


2021 ◽  
Vol 1 (4) ◽  
pp. 1-10
Author(s):  
O.V. Martynenko ◽  
◽  
V.N. Karminov ◽  
P.V. Ontikov ◽  
◽  
...  

The territory of the N.V. Tsitsin Main Botanical Garden of the Russian Academy of Sciences is subjected to significant anthropogenic stress, which has a negative effect on the state of valuable collections of tree and shrub species. Soil compaction is one of the most dangerous consequences of anthropogenic impact, expressed in increased recreational impact. In this context, the botanical garden was very concerned about the deterioration of the collection of elaeagnus species. Therefore, the soils on which these plants grow were chosen as the object of research. On this territory, three sampling plots with different degrees of anthropogenic impact were laid. Sampling plot No. 1 had the maximum anthropogenic impact. The area of medium anthropogenic impact was represented by the sampling plot No. 2. The sampling plot No. 3 where the anthropogenic impact was minimal was a reference plot. All the studied soils were classified as soddy-slightly podzolic medium loamy soils (Umbric Albeluvisols Abruptic). Fundamental differences in the morphological properties of the studied soils were that the soils located in the area of maximum anthropogenic impact, starting from a depth of 40 cm, showed gley spots, which were not found in other soils. Increased bulk density of soil in undisturbed state corresponded to increased anthropogenic impact. This led to a decrease in total pore space of soil. Soil compaction contributed to a noticeable decrease in gravimetric soil water content in upper horizons. At the same time, worsening of subsurface runoff contributed to gley-forming processes in the illuvial part of the profile. Cluster analysis revealed good grouping of the dependence of the studied indicators on the severity of anthropogenic impact. The studied indicators were separated depending on their type and position in the profile. The conducted study made it possible to assess the essential physical and hydrophysical properties of soils on the part of the territory of the Main Botanical garden of RAS that is occupied by the valuable collection of elaeagnus. Based on the results of the study, a set of measures is proposed that can significantly reduce the identified negative effects and improve the state of both the studied soils and stands growing on them in general.



2018 ◽  
Vol 28 (3) ◽  
pp. 7
Author(s):  
Alaa Al-Halfi ◽  
Mahir Zaki Al-Shammary

The study was conducted at the Botanical Garden for Department of Biology, College of Education for Pure Science (Ibn Al-Haitham), University of Baghdad for the growing season (2016-2017) in order to study the chelating compound DTPA effect at different concentrations (0, 5, 10, 15, 20) mg.L-1 and growth organizer brassinolide at concentrations (0, 0.5, 1, 1.5, 2) mg.L-1 and their interaction on some morphological properties and content of macroelement (nitrogen, phosphorus, potassium) for coriander plant. Randomized complete Block Design (RCBD) was tested with three replicates. The results showed that the application by (DTPA) act to an increase in the mean of stem diameter and the total chlorophyll content in the leaves and concentration of nitrogen and potassium at the concentration of 20 mg.L-1 while no a significant effect on phosphorus concentration compared to the control treatment. Brassinolide increased all the studied characteristics of stem diameter, total chlorophyll content in leaves, concentration of nitrogen and concentration of potassium at concentration of 2 mg.L-1, while it did not have a significant effect in phosphorus concentration compared to control treatment.The results also showed a significant interaction between the factors of experiment in all the studied characteristics at the concentrations 20 mg.L-1 from DTPA and 2 mg.L-1 from brassinolide except of phosphorous which showed a significant interaction at concentrations 20 mg.L-1 from DTPA and 1, 1.5 mg.L-1 from brassinolide.



HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 783B-783
Author(s):  
N. Shlamovitz ◽  
P. Spiegel-Roy ◽  
E. Tanne

In many cases the diagnosis of a viral disease in perennial woody plants requires indexing by grafting on indicator plants. In the case of grapevine leafroll and corky-bark diseases, indexing requires 2 to 3 years for symptom recording. Both diseases are found in all grapevine-growing countries. It would therefore be advantageous to develop a sensitive, quick, and reliable diagnostic technique. Explant shoots infected with corky-bark were micrografted onto healthy indicator rootstocks and maintained in vitro. Typical corky-bark symptoms appeared on the indicator within 8 to 12 weeks. Osmotic stress, in vitro, induced by sorbitol, enhanced leafroll symptoms. Explants expressed symptoms after 2 to 3 months of growth on these media. The advantages of these techniques are: Rapid indexing, saving of space and labor, could be performed year-round. Further experiments are underway for adaptation of the micrografting to leafroll disease and the stress method for corky-bark disease.



2019 ◽  
Vol 4 (2) ◽  
pp. 181-189
Author(s):  
Vladimir Golovko ◽  
Anastasia Belanova ◽  
Galina Zueva

A study is made on pollen emission into atmosphere of 12 species of anemophilous plants (Betula fusca Pall. ex Georgi, Betula pubescens Ehrh., Ulmus pumila L., Corylus americana Marsh., Corylus avellana L., Stipa pennata L., Poa nemoralis L., Achnatherum splendens (Trin.) Nevski, Panicum virgatum L., Lolium perenne L., Avena sativa L., Setaria maximowiczii Tzvelev & Prob.), exposed by the Central Siberian Botanical Garden, SB RAS. These plants are widely spread over the territory of the Russian Federation and produce great amounts of allergenic pollen. Estimated is the share of clusters of two and more pollen grains in the total number of pollen particles, entering the atmosphere upon blooming of plants of a given species. It is shown that although the anemophilous plants display morphological properties, that prevent the formation of clusters, substantial number of such clusters is recorded in all experimental series. In this case, the share of pollen grains could exceed 50% of the total number of pollen grains, entering the atmosphere.



2000 ◽  
Vol 179 ◽  
pp. 263-264
Author(s):  
K. Sundara Raman ◽  
K. B. Ramesh ◽  
R. Selvendran ◽  
P. S. M. Aleem ◽  
K. M. Hiremath

Extended AbstractWe have examined the morphological properties of a sigmoid associated with an SXR (soft X-ray) flare. The sigmoid is cospatial with the EUV (extreme ultra violet) images and in the optical part lies along an S-shaped Hαfilament. The photoheliogram shows flux emergence within an existingδtype sunspot which has caused the rotation of the umbrae giving rise to the sigmoidal brightening.It is now widely accepted that flares derive their energy from the magnetic fields of the active regions and coronal levels are considered to be the flare sites. But still a satisfactory understanding of the flare processes has not been achieved because of the difficulties encountered to predict and estimate the probability of flare eruptions. The convection flows and vortices below the photosphere transport and concentrate magnetic field, which subsequently appear as active regions in the photosphere (Rust & Kumar 1994 and the references therein). Successive emergence of magnetic flux, twist the field, creating flare productive magnetic shear and has been studied by many authors (Sundara Ramanet al.1998 and the references therein). Hence, it is considered that the flare is powered by the energy stored in the twisted magnetic flux tubes (Kurokawa 1996 and the references therein). Rust & Kumar (1996) named the S-shaped bright coronal loops that appear in soft X-rays as ‘Sigmoids’ and concluded that this S-shaped distortion is due to the twist developed in the magnetic field lines. These transient sigmoidal features tell a great deal about unstable coronal magnetic fields, as these regions are more likely to be eruptive (Canfieldet al.1999). As the magnetic fields of the active regions are deep rooted in the Sun, the twist developed in the subphotospheric flux tube penetrates the photosphere and extends in to the corona. Thus, it is essentially favourable for the subphotospheric twist to unwind the twist and transmit it through the photosphere to the corona. Therefore, it becomes essential to make complete observational descriptions of a flare from the magnetic field changes that are taking place in different atmospheric levels of the Sun, to pin down the energy storage and conversion process that trigger the flare phenomena.



Author(s):  
John P. Robinson ◽  
J. David Puett

Much work has been reported on the chemical, physical and morphological properties of urinary Tamm-Horsfall glycoprotein (THG). Although it was once reported that cystic fibrotic (CF) individuals had a defective THG, more recent data indicate that THG and CF-THG are similar if not identical.No studies on the conformational aspects have been reported on this glycoprotein using circular dichroism (CD). We examined the secondary structure of THG and derivatives under various conditions and have correlated these results with quaternary structure using electron microscopy.THG was prepared from normal adult males and CF-THG from a 16-year old CF female by the method of Tamm and Horsfall. CF female by the method of Tamm and Horsfall.



Author(s):  
Leon Dmochowski

Electron microscopy has proved to be an invaluable discipline in studies on the relationship of viruses to the origin of leukemia, sarcoma, and other types of tumors in animals and man. The successful cell-free transmission of leukemia and sarcoma in mice, rats, hamsters, and cats, interpreted as due to a virus or viruses, was proved to be due to a virus on the basis of electron microscope studies. These studies demonstrated that all the types of neoplasia in animals of the species examined are produced by a virus of certain characteristic morphological properties similar, if not identical, in the mode of development in all types of neoplasia in animals, as shown in Fig. 1.



Author(s):  
O. E. Bradfute

Electron microscopy is frequently used in preliminary diagnosis of plant virus diseases by surveying negatively stained preparations of crude extracts of leaf samples. A major limitation of this method is the time required to survey grids when the concentration of virus particles (VPs) is low. A rapid survey of grids for VPs is reported here; the method employs a low magnification, out-of-focus Search Mode similar to that used for low dose electron microscopy of radiation sensitive specimens. A higher magnification, in-focus Confirm Mode is used to photograph or confirm the detection of VPs. Setting up the Search Mode by obtaining an out-of-focus image of the specimen in diffraction (K. H. Downing and W. Chiu, private communications) and pre-aligning the image in Search Mode with the image in Confirm Mode facilitates rapid switching between Modes.



1953 ◽  
Vol 23 (1) ◽  
pp. 121
Keyword(s):  


1950 ◽  
Vol 34 (6) ◽  
pp. 1637-1652
Author(s):  
Thomas M. Durant ◽  
Charles R. Shuman


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