Ovarian aromatase activity in endometriosis

Aromatase is the key enzyme, which converts androgens into estrogens. To study the role of aromatase in pathogenesis of endometriosis 57 patients and 15 healthy women of reproductive age were examined. Aromatase activity was detected by reaction of estrogens on aromatase inhibitor letrozol intake. Ovarian source of detected aromatase activity was proved by suppression of reaction on letrozole intake during therapy with gonadotropin- releasing hormone agonist. Aromatase activity in recalculation on antral follicle in endometriosis patients withII–IV stages was higher than in healthy women, though total ovarian aromatase activity was not differed from control group because of low number of antral follicles in endometriosis patients. The disturbance of folliculogenesis in endometriosis is connected probably with hyperestronaemia, which has ovarian and extragonandal origin.

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Assessment of current methods of laboratory diagnosis of bacterial vaginosis

Journal of obstetrics and women s diseases ◽

10.17816/jowd63126-32 ◽

2014 ◽

Vol 63 (1) ◽

pp. 26-32

Author(s):

Kira Valentinovna Shalepo ◽

Veronika Victorovna Nazarova ◽

Yulia Nicolaevna Menukhova ◽

Tatiana Andreevna Rumyantseva ◽

Alexander Evgenievich Guschin ◽

...

Keyword(s):

Polycystic Ovary ◽

Free Testosterone ◽

Antral Follicle ◽

Laboratory Diagnosis ◽

Reproductive Age ◽

Blood Levels ◽

Aromatase Activity ◽

Women Of Reproductive Age ◽

Ovary Syndrome ◽

Antral Follicles

41 women with polycystic ovary syndrome (PCOS) and 15 healthy women of reproductive age were examined to evaluate ovarian aromatase activity. Aromatase activity was determined by the decrease of estradiol level after peroral intake of aromatase inhibitor letrosol. To examine aromatase activity of antral follicle (∆E2) was divided on the blood level of antimullerian hormone (AMH), which is corresponded to the number of antral follicles. Significant variations of aromatase activity of antral follicles in patients with PCOS were determined: in 34.1 % of women it was within physiological ranges, in 48.8 % of women it was decreased and in 17.1 % of women it was increased. Aromatase activity of antral follicles in patients with PCOS correlated with blood levels of estradiol (r = 0.67), estron (r = 0.27), free testosterone(r = 0.43), androstendion (r = 0.34) and body mass index (r = 0.30). Aromatase activity had reverse correlation with number of antral follicles. Athors suggest that the sensitivity of the ovaries to gonadotropinic stimulation is decreased in patients with PCOS and low aromatase activity.

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Should fertile women quit drinking alcohol to produce better quality oocytes?

Zygote ◽

10.1017/s0967199420000696 ◽

2020 ◽

pp. 1-3

Author(s):

Burcu Ozbakir ◽

Pinar Tulay

Keyword(s):

Alcohol Consumption ◽

Young Women ◽

Antral Follicle ◽

Antral Follicle Count ◽

Control Group ◽

Social Drinkers ◽

Antral Follicles ◽

Healthy Women ◽

Significant Difference

Summary Alcohol consumption has long been shown to affect both fetal health and pregnancy. In this study, antral follicle count, maturation level of oocytes including morphological assessment and number of metaphase I (MI), metaphase II (MII) and germinal vesicle (GV) stage oocytes obtained from young women (age < 30 years old) with or without alcohol consumption were investigated. In total, 20 healthy women who were social drinkers and 36 healthy women who do not consume alcohol were involved in this study. Women in both study and control groups were undergoing controlled ovarian stimulation. The antral follicle count and the number and quality of the oocytes retrieved were evaluated and recorded. In total, 635 antral follicles, 1098 follicles and 1014 oocytes with 820 MII, 72 MI and 78 GV stage oocytes were collected from the social drinkers. In the control group, 628 antral follicles, 1136 follicles and 1085 oocytes with 838 MII, 93 MI and 102 GV stage oocytes were evaluated. The results of this study showed that the antral follicle count was very similar in both groups. The number of oocytes and MII stage oocytes was slightly higher in the control group, although it was not a significant difference. This study showed that although the consumption of alcohol may have adverse effects post-implantation, it may not have a solid effect during oogenesis in young women. The results of this study are especially important in clinical settings as some women who are social drinkers undergo in vitro fertilization treatments.

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ESTIMATION TO EFFICIENCY OF THE MULTIFUNCTION METHOD ОF ENDOMETRIOSIS OVARIЕS TREATMENT

Wiadomości Lekarskie ◽

10.36740/wlek202005106 ◽

2020 ◽

Vol 73 (5) ◽

pp. 868-872

Author(s):

Iryna M. Nikitina ◽

Volodymyr I. Boiko ◽

Svitlana A. Smiian ◽

Tetiana V. Babar ◽

Natalia V. Kalashnyk ◽

...

Keyword(s):

Gonadotropin Releasing Hormone ◽

Inhibin B ◽

Reproductive Age ◽

Sexual Life ◽

Control Group ◽

Women Of Reproductive Age ◽

Releasing Hormone ◽

Antral Follicles ◽

Group I ◽

Laparoscopic Removal

The aim: The aim of the study was to improve the results of treatment of patients with endometriosis by using a combination method of therapy. Materials and methods: For two years, 136 women of reproductive age who underwent laparoscopic surgeries for ovarian endometriosis were monitored: Group I (n = 24) did not receive any hormonal treatment in the perioperative period; Group II (n = 32) – received gonadotropin-releasing hormone agonists within 3 months after surgery; Group III (n = 80) prior to laparoscopic removal of the ovarian cyst used gonadotropin-releasing hormone agonists – Triptorelin 3.75 mg intramuscularly for 2 months, as well as three months after surgery. The control group consisted of 30 healthy women of reproductive age with regular menstrual periods. All patients underwent transvaginal ultrasound, counting the number of antral follicles before and after treatment. Serum hormone levels (FSH, prolactin, thyrotropic hormone, anti-Mullerian hormone, inhibin B) were determined by enzyme-linked immunosorbent assay on Cobas e-411 analyzer (Roche Diagnostics, Switzerland) on day 2-3 of the menstrual cycle and on day 2–3 of the first menstrual period after the end of treatment. Laparoscopic removal of the cyst was performed with exfoliation of the cyst, hemostasis on the wound surface of the bed of the cyst was performed with a bipolar electrocoagulator. Bipolar coagulation and resection of the ovarian tissue with no potential was used during surgical treatment of the ovaries, which made it possible to preserve the intact portion of the ovary as much as possible. Results: Analysis of ovarian reserve indices, namely number of antral foliculs, number of antral follicles, AMG, and inhibin B levels in all examined patients with ovarian endometriomas were significantly lower than those of the control group before the start of treatment: in the ovarian endometrial group group 1.26 times (p <0.01), inhibin B – 1.5 times (p <0.01), the number of antral follicles – 1.2 times (p <0.01), due to the development dystrophic changes of the follicular apparatus due to prolonged compression, hypoxia, fibrosis in the ovaries. Patients who planned pregnancy were advised to have an active sexual life before menstruation was restored. In 23 (46.9%) of 49 patients who had reproductive plans, pregnancy occurred without first menstruation after a course of gonadotropin-releasing hormone agonists, 12 (24.5%) women became pregnant during the first three menstrual cycles. Extracorporeal fertilization was recommended for women who did not have pregnancy within 6 months of surgery. For two years in women who did not plan pregnancy, recurrence of endometriosis was not observed. Conclusions: The combination of laparoscopic treatment with gonadotropin-releasing hormone agonists in patients with endometriosis with infertility allowed to restore reproductive function in 71.4% of women, which indicates the effectiveness of the treatment method used. In addition, it helps to achieve lasting remission and addresses the socio-social problems of women’s health and maternity.

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Relationship of the ovarian reserve with autoimmune thyroid diseases in the reproductive period

Terapevticheskii arkhiv ◽

10.26442/00403660.2019.10.000095 ◽

2019 ◽

Vol 91 (10) ◽

pp. 14-18

Author(s):

O R Grigoryan ◽

N S Krasnovskaya ◽

R K Mikheev ◽

I S Yarovaia ◽

E N Andreeva ◽

...

Keyword(s):

Ovarian Reserve ◽

Inhibin B ◽

Reproductive Age ◽

Fmr1 Gene ◽

Normal Range ◽

Women Of Reproductive Age ◽

Antral Follicles ◽

Antithyroid Antibodies ◽

Healthy Women ◽

The Status

Aim. To compare ovarian reserve in healthy women of reproductive age - carriers of antithyroid antibodies (ATA) and in healthy women of reproductive age negative for ATA. Materials and methods. 70 healthy women of young reproductive age in the state of euthyroidism (from 18 to 38 years old) were examined. Participants were divided into equal groups (n=35) depending on the status of the presence of antithyroid antibodies (AT-TPO, AT-TG). On the 2nd-4th days of the menstrual cycle, the following markers of the ovarian reserve were determined: serum levels of anti-Müllerian hormone (AMG), inhibin B, FSH, LH, estradiol, testosterone and progesterone, as well as ultrasound parameters - the number of antral follicles and the volume of the ovaries. In addition, to determine the predisposition to premature ovarian failure, an analysis was performed to the number of CGG repeats in the FMR1 gene. Results and discussion. Statistically significantly differs such parameters as the level of estradiol and testosterone, while the differences were not clinically significant. All the parameters evaluated were within the normal range, the main predictors of the ovarian reserve (levels of AMG and inhibin B, the number of antral follicles) remained in the normal range. An increase in the number of repeats of CGG in the FMR1 gene was not detected in any of the participants in the study. Conclusion. In healthy young reproductive age women, the status of ATA does not have a direct effect on the ovarian reserve.

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The Quality of Life in the Women of Reproductive Age as Patients with Active Forms of Tuberculosis

Journal of New Medical Technologies ◽

10.12737/3314 ◽

2014 ◽

Vol 21 (1) ◽

pp. 57-63 ◽

Cited By ~ 1

Author(s):

Камаев ◽

I. Kamaev ◽

Русановская ◽

G. Rusanovskaya ◽

Шпрыков ◽

...

Keyword(s):

Quality Of Life ◽

Pulmonary Tuberculosis ◽

Life Quality ◽

Reproductive Age ◽

Control Group ◽

Main Process ◽

Women Of Reproductive Age ◽

Healthy Women ◽

Tuberculosis Disease

The authors assessed the quality of life of 263 women of reproductive age. The main group consisted of 139 women aged o18-44 as the patients with active pulmonary tuberculosis disease treated in 2011-2012, and the other group of comparison consisted of 124 healthy women of the same age. To assess the life quality the authors used Russian version of the SF-36 which included questions of medico- social nature. It was proved that all the life quality parameters of the women suffering from tuberculosis disease were reduced in comparison with the control group of the healthy women. The only exception was the pain syndrome for which the statistical significance wasn’t reached at all and the physical functioning was assessed at the lowest level. The life quality problem was identified almost in half of the women that proved by the obtained data. Analysis of quality of life of the women established that 25-34 years respondents had the highest reducing of the rates. The analyzed group of patients differed from the low level of the socio- economic independence relatively favorable structure of clinical tuberculosis forms but with a high frequency of tubercle bacilli discharging and detection of lung tissue destructing. Generally, women with pulmonary tuberculosis had numerous risk factors for disease and co-morbidities aggravating the main process. The unfavorable health and social hygienic characteristics of the analyzed group of patients contribute to late making a diagnosis and spreading the infection among the population, including the women of reproductive age. Therefore health care professionals need to make efforts to promote healthy lifestyle and prevention of tuberculosis with this contingent that will allow to prevent new cases of disease and to reduce morbidity. The aim of any disease treatment, including tuberculosis should be considered as acts to improve the life quality of patients in case of positive clinical dynamics. Therefore it is important to guide medical practice for the patients’ psychosomatic status correcting in the early stages of disease that will be able to solve health problems more successfully. Thereby, the authors think it is necessary to organize services; to open offices where patients could get psychosocial correction and rehabilitation, to train the specialists.

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Methodological approaches to the definition of ovarian aromatase in polycystic ovary syndrome

Journal of obstetrics and women s diseases ◽

10.17816/jowd65154-61 ◽

2016 ◽

Vol 65 (1) ◽

pp. 54-61 ◽

Cited By ~ 3

Author(s):

Ekaterina M Timofeeva ◽

Elena V Misharina ◽

Igor P Nikolaenkov ◽

Yanina A Samoylovich ◽

Natalya N Tkachenko

Keyword(s):

Polycystic Ovary Syndrome ◽

Polycystic Ovary ◽

Reproductive Age ◽

Aromatase Activity ◽

Women Of Reproductive Age ◽

Ovary Syndrome ◽

Antral Follicles ◽

Methodological Approaches ◽

Definition Of ◽

Relative Deficiency

In order to study ovarian flavor ase activity in polycystic ovary syndrome (PCOS) examined 49 patients with PCOS and 33 healthy women of reproductive age. Aromatase activity determined using estradiol/number of antral follicles in both ovaries ratio (Е2/n). Values ovarian aromatase positively correlated (p < 0.05) with the results of its determination by a test with the aromatase inhibitor letrozole in PCOS. Reduced aromatase activity of antral follicles was present in 59 % of patients with PCOS. These data indicate that Е2/n ratio allows to evaluate ovarian aromatase and that an absolute or relative deficiency of ovarian aromatase underlies the pathogenesis of PCOS.

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Ovarian aromatase activity in polycystic ovarian syndrome

Journal of obstetrics and women s diseases ◽

10.17816/jowd63110-16 ◽

2014 ◽

Vol 63 (1) ◽

pp. 10-16 ◽

Cited By ~ 2

Author(s):

Igor Pavlovich Nikolayenkov ◽

Vladimir Vsevolodovich Potin ◽

Marina Anatolyevna Tarasova ◽

Natalya Nikolayevna Tkachenko ◽

Maria Igorevna Yarmolinskaya ◽

...

Keyword(s):

Polycystic Ovarian Syndrome ◽

Polycystic Ovary ◽

Free Testosterone ◽

Antral Follicle ◽

Reproductive Age ◽

Blood Levels ◽

Aromatase Activity ◽

Ovary Syndrome ◽

Antral Follicles ◽

Ovarian Syndrome

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THE SPECIFICITY OF THE OVARIAN RESERVE OF WOMEN WITH CHRONIC SALPINGOOPHORITIS

Kuban Scientific Medical Bulletin ◽

10.25207/1608-6228-2018-25-6-119-126 ◽

2018 ◽

Vol 25 (6) ◽

pp. 119-126

Author(s):

V. A. Novikova ◽

F. R. Autleva ◽

A. A. Sorochenko ◽

D. I. Fayzullina ◽

E. V. Nurgalieva

Keyword(s):

Ovarian Reserve ◽

Reproductive Period ◽

Main Group ◽

Reproductive Age ◽

Control Group ◽

Regression Equations ◽

Women Of Reproductive Age ◽

Antral Follicles ◽

Preconception Consultation ◽

The Impact

Aim. The research was conducted for the assessment of the impact of chronic salpingoophoritis on the ovarian reserve of women in various phases of reproductive age.Materials and methods. A prospective, controlled and open cohort study was performed in 2013-2018 (n=202). The main group consisted of women with chronic salpingoophoritis (ChrSO) who applied for preconception consultation (n=138). In accordance with the reproductive age phase, the main group was divided into subgroups: the early reproductive age period (ERP, n=44), the peak reproductive age period (PRP, n=56), the late reproductive period (LRP, n=38). The control group consisted of conditionally healthy women of reproductive age (n=64). The ovarian reserve (OR) was estimated on the basis of the serum level of antimullerian hormone (AMH), inhibin B, estradiol, follicle stimulating hormone (FSH), an ultrasoundbased assessment of the number of antral follicles (AF), and the ovarian volume. Results. The age of women ranged from 18 to 40 years. Based on the discriminant analysis, it was found that the main indicators determining the specificity of the OR in ChrSO, depending on the phase of reproductive age, are the number of antral follicles, estradiol level and AMH (Wilks’ lambda = 0.35503, p<0.0001). The specificity of the OR of women with ChrSO (difference from the control group), regardless of the phase of reproductive age, initially and when evaluated after 6 months, is determined by the number of AF and the level of estradiol and AMH; the number of AF and AMH is determined with a similar estimate after 12 months. The specificity of the OR in ChrSO, which is dependent on the reproductive age phase, has been proved through the analysis with the neural networks training(the proportion of correct answers is more than 80%). The linear relationships were established between the values of each OR parameter in women with ChrSO. Initially, when estimating after 6 and 12 months, linear regression equations were calculated, allowing the values of individual OR parameters to be calculated over 6 and 12 months.Conclusion. Chronic salpingoophoritis (ChrSO) is associated with a decrease in ovarian reserve in women of reproductive age. The effect of ChrSO on some parameters of the ovarian reserve depends on the age phase of the reproductive period, which increases with time (after 6, 12 months). The presence of ChrSO in women planning future pregnancies requires preventive and therapeutic measures aimed at preserving the ovarian reserve and the preferred implementation of fertility in early reproductive age before the ovarian reserve starts to decline.

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UTERINE FIBROIDS IMPACT ON FERTILITY AND PREGNANCY OUTCOME

EUREKA Health Sciences ◽

10.21303/2504-5679.2020.001205 ◽

2020 ◽

Vol 2 ◽

pp. 52-58

Author(s):

Olena Doroha ◽

Mykola Iarotskyi ◽

Iryna Iarotska ◽

Liudmyla Semeniuk ◽

Olga Nikitina

Keyword(s):

Sex Hormones ◽

Uterine Leiomyoma ◽

Uterine Fibroids ◽

Statistical Processing ◽

Main Group ◽

Reproductive Age ◽

Control Group ◽

Women Of Reproductive Age ◽

Healthy Women ◽

Obstetric History

The clinical confirmation of the relationship between uterine leiomyoma and female infertility is of interest to scholars. The aim of our research was to study obstetric history and the level of sex hormones in women of reproductive age with leiomyoma. Materials and methods. The main group consisted of 90 women of reproductive age with uterine leiomyoma, the control group - 45 healthy women. Gynaecological and obstetric history, hormone content in peripheral blood and pelvic vessels (estradiol, progesterone) were analysed. Statistical processing of clinical material was performed using Microsoft Excel, Statistica 7.0, and Statistica 8.0 for Windows. Results. The frequency of infertility in the surveyed women with LM was 18.9 %. One in three patients in the main group (33.3 %) did not give birth, which was statistically different from the healthy women group. In this case, 25.6 % of women in the main group did not use any methods of contraception. It was found that 51.11 % of patients with LM had threatened miscarriage in their history, 37.5 % – complicated delivery. Pathological changes in the concentrations of estradiol and progesterone in the preferential blood and blood vessels of women with fibroids have been identified. We have found that the content of sex hormones in the local bloodstream has a reliable relationship with the location of the myomatous node. Conclusion. The obtained results may indicate that leiomyoma contributes to the reduction of fertility. This is evidenced by the fact that women with LM are more likely to have the risk of miscarriage, complicated delivery and delivery by caesarean section, including complicated one. The findings have made a significant contribution to the further development of effective strategies for the prevention and treatment of infertility in women with fibrotic uterine lesions.

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Tryptophan-Niacin Metabolism in Rat with Puromycin Aminonucleoside-Induced Nephrosis

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831.76.1.28 ◽

2006 ◽

Vol 76 (1) ◽

pp. 28-33 ◽

Cited By ~ 7

Author(s):

Yukari Egashira ◽

Shin Nagaki ◽

Hiroo Sanada

Keyword(s):

Body Weight ◽

Urinary Excretion ◽

Enzyme Activities ◽

Treated Group ◽

Control Group ◽

Puromycin Aminonucleoside ◽

Low Level ◽

Key Enzyme

We investigated the change of tryptophan-niacin metabolism in rats with puromycin aminonucleoside PAN-induced nephrosis, the mechanisms responsible for their change of urinary excretion of nicotinamide and its metabolites, and the role of the kidney in tryptophan-niacin conversion. PAN-treated rats were intraperitoneally injected once with a 1.0% (w/v) solution of PAN at a dose of 100 mg/kg body weight. The collection of 24-hour urine was conducted 8 days after PAN injection. Daily urinary excretion of nicotinamide and its metabolites, liver and blood NAD, and key enzyme activities of tryptophan-niacin metabolism were determined. In PAN-treated rats, the sum of urinary excretion of nicotinamide and its metabolites was significantly lower compared with controls. The kidneyα-amino-β-carboxymuconate-ε-semialdehyde decarboxylase (ACMSD) activity in the PAN-treated group was significantly decreased by 50%, compared with the control group. Although kidney ACMSD activity was reduced, the conversion of tryptophan to niacin tended to be lower in the PAN-treated rats. A decrease in urinary excretion of niacin and the conversion of tryptophan to niacin in nephrotic rats may contribute to a low level of blood tryptophan. The role of kidney ACMSD activity may be minimal concerning tryptophan-niacin conversion under this experimental condition.

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