scholarly journals Morphological substantiation of pathogenetic therapyof external genital endometriosis

2016 ◽  
Vol 65 (6) ◽  
pp. 100-103
Author(s):  
Irina V Markar’yan ◽  
Natalia V Yermolova ◽  
Nina A Drukker ◽  
Vladimir Y Mazhugin ◽  
Alexander N Rymasheuski ◽  
...  
Keyword(s):  
Endometrial Cancer ◽  
Histological Examination ◽  
Morphological Study ◽  
Morphological Changes ◽  
Eutopic Endometrium ◽  
Morphological Heterogeneity ◽  
Secretory Type ◽  
The Difference ◽  
Cytoplasmic Structures ◽  
Inflammatory Changes

With the help of morphological study examined endometrioid heterotopias and eutopic endometrium in external genital endometriosis. Histological examination of surgical specimens of endometrial cancer were detected in endometrioid heterotopia, in eutopic endometrium occurred glandular-cystic hyperplasia of the proliferative and secretory type. Have been identified in the heterotopias expressed angiomatosis in patients with III-IV stages of endometriosis. It is proved that inflammatory changes in endometrioid heterotopias more pronounced with stage I-II stages of endometriosis, whereas hyalinosis sclerosis and how the outcomes of the process prevalent in patients with stage III-IV. The difference parameters in ectopic and eutopic endometrium shows marked morphological heterogeneity of both nuclear and cytoplasmic structures. Morphological changes in the heterotopias are intended to justify Dienogest (2 mg) for the treatment of external genital endometriosis.

scholarly journals Comparative pathomorphological research of hydroxycarbamide and hydrea

2019 ◽  
Vol 18 (2) ◽  
pp. 66-73
Author(s):  
I. B. Merkulova ◽  
T. V. Abramova ◽  
O. I. Konyaevа ◽  
N. Yu. Kulbachevskaya ◽  
N. P. Ermakova ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Histological Examination ◽  
Morphological Changes ◽  
Import Substitution ◽  
Maximum Tolerated Dose ◽  
Male Rats ◽  
Internal Organs ◽  
Hematoxylin And Eosin ◽  
Starch Paste ◽  
Inflammatory Changes

Introduction . In accordance with Russian Federal Program of import substitution of foreign medicines replacement for high-quality Russian drugs in Russia reproduced нydroxycarbamide (HC), which passed preclinical toxicological and pathomorphological testing in comparison with hydrea (HD), producted by Italy.The aim . The aim of present study was the comparative evaluation of HC and HD effect on the internal organs of rats for the clearing up of their identity according to the morphological criterions. Materials and methods. 70 non-inbred male rats, by 10 rats per group, were used. HC and HD were administered to rats oral daily for 5 days in the same total doses correspond with to the 2, 1 and 1 / 2 maximum tolerated dose. In the same regime control rats were oral administered with 1 % starch paste solution. Of animals were removed from the experience on days 3 and 30 after the end of the administration of the drugs. The macroscopic and histological examination of internal organs were perform by routine methods, including fixation of the material in 10 % formalin and staining of sections with hematoxylin and eosin. The histological preparations of the internal organs was analyzed in the light microscope at magnifications of 100, 400, 1000. Results . HС, as well as HD, in total doses of 3000 and 1500 mg / kg at the 3 day after the end of the introduction caused different degrees of similar morphological changes in rat internal organs: moderate hypoplasia in the thymus, bone marrow and spleen, destructive and inflammatory changes in the stomach, duodenum and kidney. At the 30 day after the application of both drugs morphological changes in the thymus, the bone marrow and the spleen disappeared completely, residual morphological changes persisted in the duodenum and kidneys; in the testes of rats – moderate atrophic changes. HС, as well as HD in total dose 750 mg / kg did not cause changes in the internal organs of the rats. Conclusion . Based on the results of macroscopic and histological examination the conclusion about of the identity of the influence of HC and HD on the internal organs of rats was made. 

Placental inflammatory changes and their association with the vaginal microbiota before delivery

2021 ◽  
Vol 70 (1) ◽  
pp. 59-68
Author(s):  
Dzhamilya G. Dadayeva ◽  
Alexandra K. Sosnina ◽  
Tatyana G. Tral ◽  
Gulrukhsor Kh. Tolibova ◽  
Olga V. Budilovskaya ◽  
...  
Keyword(s):  
Histological Examination ◽  
Vaginal Discharge ◽  
Vaginal Microbiota ◽  
Amniotic Cavity ◽  
Membrane Rupture ◽  
Opportunistic Bacteria ◽  
Prolonged Labor ◽  
Adverse Pregnancy ◽  
Prelabor Rupture Of Membranes ◽  
Inflammatory Changes

Hypothesis/aims of study: Infection of the amniotic cavity and placenta is one of the leading causes of adverse pregnancy outcomes. In the majority of cases, intra-amniotic infection is associated with the normal microbiota of the lower urogenital tract. The aim of the study was to explore the relationships between the placental inflammatory changes, vaginal microbiota and labor course. Study design, materials and methods: We examined 124 women at 37-41 weeks of gestation. The vaginal discharge at admission was taken for microbiological evaluation, with the delivered placenta sent for histological examination. Results: In 17.7% of cases, histological examination of the placenta revealed inflammatory changes. A statistically significant correlation was noted between the placental inflammatory changes and Staphylocossus spp. presence in the vaginal discharge at admission (р = 0.0004). The placental inflammatory changes were associated with the membrane rupture to delivery interval more than 6 hours (р = 0.01) and the labor duration more than 7 hours (р = 0.0004). Prelabor rupture of membranes did not significantly affect the placental inflammatory changes (p = 1.0). Conclusion: Predisposing factors for the development of ascending bacterial infection of the placenta are an abnormal vaginal microbiota with the presence of opportunistic bacteria before delivery, a long membrane rupture to delivery interval, and a prolonged labor.

Ultrastructure of the podia of Amphiura chiajei and Amphiura filiformis and their role in feeding

2006 ◽  
Vol 86 (4) ◽  
pp. 817-822 ◽  
Author(s):  
John K. Keogh ◽  
Brendan F. Keegan
Keyword(s):  
Chemical Composition ◽  
Morphological Study ◽  
Secretory Cells ◽  
Suspension Feeding ◽  
Head Region ◽  
Feeding Styles ◽  
Amphiura Filiformis ◽  
The Difference ◽  
Mucus Cells ◽  
Deposit Feeding

Morphological study of the podia of the suspension feeding Amphiura filiformis and the deposit feeding Amphiura chiajei revealed sensory–secretory complexes in the podial epidermis, consisting of four cells, two secretory and two sensory. Large mucus cells were found in association, but not exclusively, with the sensory–secretory complexes. In A. filiformis, mucus cells stained positively for both acid and neutral mucopolysaccharides, while, in A. chiajei, these cells stained only for acid mucopolysaccharides. The surfaces of the arm podia in A. chiajei were relatively smooth, while the arm podia of A. filiformis bear papillae. The sensory–secretory complexes open through numerous paired pores, with each pair having an intervening cilium. Pores were restricted to the podial tip in A. chiajei, while in A. filiformis they are concentrated on the podial tip and on the papillae. Amphiura chiajei shows very little differentiation of the podia along the length of the arm. In A. filiformis, the distal podia have papillae throughout their entire length, with pores being found on the head region and the papillar tips. Here, the papillae are oriented in such a way (i.e. facing inward towards the ventral arm plate) as to increase the area of the filtering surface of the podium, serviced by the sticky secretions from the sensory–secretory complexes. The proximal podia are relatively simple in structure and are thought to function more in the transportation of mucus wrapped particles to the mouth rather than in their capture. The difference in structure of the podia and chemical composition of podial secretory cells are taken to reflect the difference in feeding styles of the two species.

scholarly journals CONDITION OF FETOPLACENTIAL COMPLEX IN PATIENTS AFTER APPLICATION OF ART

2020 ◽  
pp. 7-13
Author(s):  
S.Sh. Issenova ◽  
G.Zh. Bodykov ◽  
A.S. Shukirbaeva ◽  
M.O. Kubesova ◽  
L. Ziyat ◽  
...  
Keyword(s):  
Blood Flow ◽  
Cardiac Surgery ◽  
Retrospective Analysis ◽  
Histological Examination ◽  
The State ◽  
Placental Insufficiency ◽  
Archival Material ◽  
Spontaneous Pregnancy ◽  
Comparative Group ◽  
Inflammatory Changes

A retrospective analysis was carried out according to the archival material of the center of perinatology and pediatric cardiac surgery in Almaty for 2017-2018. The analysis was carried out in 2 groups: the main group - 250 histories of women giving birth after IVF and the comparative group - 250 histories of women giving birth to spontaneous pregnancy. The condition of the feto-placental complex was assessed by studying the data of ultrasound, Dopplerometry, CTG, histological examination. The study shows that in women after the use of ART, the absence of pronounced blood flow disorders in the mother-placenta-fetus system according to Dopple-rometry is important and significant, which contributes to the favorable course of pregnancy and the state of the fetus, as well as the presence of morphological signs of placental insufficiency in 68% of cases inflammatory changes in the placenta in 57% of cases of varying severity.

scholarly journals Senescent Accelerated Prone 8 (SAMP8) Mice as A Model of Age Dependent Neuroinflammation

2020 ◽  
Author(s):  
Andrés Fernández ◽  
Elena Quintana ◽  
Patricia Velasco ◽  
Belén de Andrés ◽  
Maria Luisa Gaspar ◽  
...  
Keyword(s):  
Choroid Plexus ◽  
Inflammatory Cytokines ◽  
Hippocampal Formation ◽  
Morphological Changes ◽  
Interleukin 1 ◽  
Brain Parenchyma ◽  
Age Related ◽  
Inflammatory Changes ◽  
Samp8 Mice ◽  
The Brain

Abstract Background: Aging and age related diseases are strong risk factors for the development of neurodegenerative diseases. Neuroinflammation (NIF), as the brain's immune response, plays an important role in aged associated degeneration of central nervous system (CNS). The need of animal models that will allow us to understand and modulate this process is required for the scientific community. Methods: We have analyzed aging-phenotypical and inflammatory changes of brain myeloid cells (bMyC) in a senescent accelerated prone aged (SAMP8) mouse model, and compared with their resistant to senescence control (SAMR1). We have performed morphometric methods to evaluate the architecture of cellular prolongations and analyzed Iba1+ clustered cells with aging. To analyse specific constant brain areas we have performed stereology measurements of Iba1+ cells in the hippocampal formation. We have isolated bMyC from brain parenchyma (BP) and choroid plexus and meningeal membranes (m/Ch), and analyzed their response to systemic LPS- driven inflammation.Results: Aged 10 month old SAMP8 mice presents many of the hallmarks of aging-dependent neuroinflammation when compared with their senescence resistant control (SAMR1); ie, increase of protein aggregates, presence of Iba1+ clusters, but not increase in the number of Iba1+ cells. We have further observed and increased of main inflammatory mediator IL-1β, and augment of border MHCII+Iba1+ cells. Isolated CD45+ bMyC from brain parenchyma (BP) and choroid plexus and meningeal membranes (m/Ch) have been analyzed showing that there is not significant increase of CD45+ from the periphery. Our data support that aged-driven pro-inflammatory cytokine interleukin 1 beta (IL1β) transcription is mainly enhanced in CD45+BP cells. Furthermore, we are showing that LPS-driven systemic inflammation produces inflammatory cytokines mainly in the border bMyC, sensed to a lesser extent by the BP bMyC, and is enhanced in aged SAMP8 compared to control SAMR1.Conclusion: Our data validate the SAMP8 model to study age-associated neuroinflammatory events, but careful controls for age and strain are required. These animals show morphological changes in their bMyC cell repertoires associated to age, corresponding to an increase in the production of main pro inflammatory cytokines such as IL-1β, which predispose the brain to an enhanced inflammatory response after LPS-systemic challenge.

scholarly journals River flow and sediment transport simulation based on a curvilinear and rectilinear grid modelling approach – a comparison study

2017 ◽  
Vol 17 (5) ◽  
pp. 1325-1334 ◽  
Author(s):  
G. G. Morianou ◽  
N. N. Kourgialas ◽  
G. P. Karatzas ◽  
N. P. Nikolaidis
Keyword(s):  
Sediment Transport ◽  
River Flow ◽  
Morphological Changes ◽  
Field Measurements ◽  
Grid Model ◽  
Curvilinear Grid ◽  
Saint Venant Equations ◽  
Flow And Sediment Transport ◽  
Downstream Section ◽  
The Difference

In the present work, a two-dimensional (2D) hydraulic model was used for the simulation of river flow and sediment transport in the downstream section of the Koiliaris River Basin in Crete, Greece, based on two different structured grids. Specifically, an important goal of the present study was the comparison of a curvilinear grid model with a rectilinear grid model. The MIKE 21C model has been developed to simulate 2D flows and morphological changes in rivers by using either an orthogonal curvilinear grid or a rectilinear grid. The MIKE 21C model comprises two parts: (a) the hydrodynamic part that is based on the Saint-Venant equations and (b) the morphological change part for the simulation of bank erosion and sediment transport. The difference between the curvilinear and the rectilinear grid is that the curvilinear grid lines follow the bank lines of the river, providing a better resolution of the flow near the boundaries. The water depth and sediment results obtained from the simulations for the two different grids were compared with field observations and a series of statistical indicators. It was concluded that the curvilinear grid model results were in better agreement with the field measurements.

scholarly journals Morphological Changes of Placenta in Preeclampsia

1970 ◽  
Vol 7 (1) ◽  
pp. 49-54 ◽  
Author(s):  
Segupta Kishwara ◽  
Shamim Ara ◽  
Khandaker Abu Rayhan ◽  
Mahamuda Begum
Keyword(s):  
Morphological Changes ◽  
Medical College ◽  
Group A ◽  
Different Shapes ◽  
The Difference ◽  
To Come ◽  
Group B ◽  
Larger Sample ◽  
Normal Placenta ◽  
Human Placentae

Context: Preeclampsia is regarded as a risk factor in pregnancy and it leads to placental insufficiency. This, in turn causes both maternal and fetal morbidity and mortality. The present study intends to compare the morphological changes of placenta in preeclampsia with that of normal placenta. Study design: A descriptive type of study. Place and period of study: Department of Anatomy, Dhaka Medical College, Dhaka, from August 2005 to June 2006. Materials: 60 human placentae, 30 were from normal pregnant women as control and 30 from pregnancies complicated by preeclampsia were taken for this study. Method: Samples were grouped as Group A and Group B on the basis of presence or absence of preeclampsia. All samples were studied morphologically. Result: Different shapes of placenta were found with some having accessory lobes in both groups. There was significant reduction (p< 0.001) in diameter and volume of placenta in Group B. The thickness of placenta was reduced in Group B but the difference did not reach a significant level. The number of cotyledon was significantly reduced in Group B (p<0.05). Conclusion: In this study, it was found that the preeclamptic placentae underwent definite morphological changes. These changes seemed to be the result of insufficiency of placenta in preeclampsia. However, further histological and morphometric placental study in a larger sample has to be conducted to come up with a conclusive decision. Key Words: Placenta, Preeclampsia, Morphology.   doi: 10.3329/bja.v7i1.3026 Bangladesh Journal of Anatomy January 2009, Vol. 7 No. 1 pp. 49-54

scholarly journals Could Galectin 3 Be a Good Prognostic Factor in Endometrial Cancer?

Diagnostics ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 635
Author(s):  
Aneta Cymbaluk-Płoska ◽  
Paula Gargulińska ◽  
Sebastian Kwiatkowski ◽  
Ewa Pius-Sadowska ◽  
Bogusław Machaliński
Keyword(s):  
Endometrial Cancer ◽  
Prognostic Factor ◽  
Univariate Analysis ◽  
Disease Free Survival ◽  
Free Survival ◽  
Median Serum ◽  
Galectin 3 ◽  
Node Metastases ◽  
The Difference

Galectin 3 is a modulator of several basic biological functions. It may be involved in the development of obesity and type 2 diabetes—risk factors of endometrial cancer. The study involved 144 patients, after abrasion due to postmenopausal bleeding. Galectin 3 concentrations were quantified in serum by multiplex fluorescent bead-based immunoassays. Median serum galectin 3 concentrations revealed significant differences between FIGO III and IV vs. FIGO I and II patients. Statistically higher concentrations were reported for patients with lymph node metastases compared to patients without it (p = 0.001) as well as in patients with lymphovascular space invasion compared to patients without LVSI (p = 0.02). No statistically significant differences were observed for median of galectin 3 levels depending on the surgical procedure (laparoscopy vs. laparotomy, p = 0.0608). Patients with galectin 3 levels exceeding the median value were characterized by overall survival being shorter by 11.9 months. High levels of galectin 3 were correlated with shorter disease-free survival, the difference is up to 14.8 months. Galectin 3 can be an independent prognostic factor in patients with endometrial cancer. Among the recognized prognostic factors and the concentrations of the galectin 3 marker at the adopted time points, the univariate analysis showed a significant effect of staging, grading, and cutoff galectin 3 on the OS. For multivariate analysis, the galectin 3 cutoff point had the greatest significant impact on OS.

scholarly journals Application of ECIS to Assess FCCP-Induced Changes of MSC Micromotion and Wound Healing Migration

Sensors ◽  
2019 ◽  
Vol 19 (14) ◽  
pp. 3210 ◽  
Author(s):  
Sheng-Po Chiu ◽  
Yu-Wei Lee ◽  
Ling-Yi Wu ◽  
Tse-Hua Tung ◽  
Sofia Gomez ◽  
...  
Keyword(s):  
Time Series ◽  
Wound Healing ◽  
Cell Migration ◽  
Morphological Changes ◽  
Mitochondrial Dynamics ◽  
Human Mesenchymal Stem Cells ◽  
Atp Synthesis ◽  
Extracellular Flux ◽  
Sigmoid Curve ◽  
The Difference

Electric cell-substrate impedance sensing (ECIS) is an emerging technique for sensitively monitoring morphological changes of adherent cells in tissue culture. In this study, human mesenchymal stem cells (hMSCs) were exposed to different concentrations of carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP) for 20 h and their subsequent concentration-dependent responses in micromotion and wound healing migration were measured by ECIS. FCCP disrupts ATP synthesis and results in a decrease in cell migration rates. To detect the change of cell micromotion in response to FCCP challenge, time-series resistances of cell-covered electrodes were monitored and the values of variance were calculated to verify the difference. While Seahorse XF-24 extracellular flux analyzer can detect the effect of FCCP at 3 μM concentration, the variance calculation of the time-series resistances measured at 4 kHz can detect the effect of FCCP at concentrations as low as 1 μM. For wound healing migration, the recovery resistance curves were fitted by sigmoid curve and the hill slope showed a concentration-dependent decline from 0.3 μM to 3 μM, indicating a decrease in cell migration rate. Moreover, dose dependent incline of the inflection points from 0.3 μM to 3 μM FCCP implied the increase of the half time for wound recovery migration. Together, our results demonstrate that partial uncoupling of mitochondrial oxidative phosphorylation reduces micromotion and wound healing migration of hMSCs. The ECIS method used in this study offers a simple and sensitive approach to investigate stem cell migration and its regulation by mitochondrial dynamics.

Changes in Teat Morphology (Doka Phenomenon) and Estrus Prediction in Riverine Buffaloes (Bubalus bubalis)

2019 ◽  
Author(s):  
S K Joshi ◽  
T K Mohanty ◽  
A Kumaresan ◽  
M Bhakat ◽  
S Sathapathy
Keyword(s):  
Morphological Changes ◽  
Bubalus Bubalis ◽  
North India ◽  
Research Centre ◽  
Murrah Buffaloes ◽  
Group I ◽  
Before And After ◽  
Group Ii ◽  
The Difference ◽  
The Relationship

The objective of the study was to characterize the term Doka and to capture morphological changes in teat to find out the relationship between Doka and onset of estrus in Murrah buffaloes (Bubalus bubalis). The Murrah buffaloes maintained at of Livestock Research Centre (LRC), ICAR-NDRI, Karnal were classified into two groups based on the lactation number for the study of Doka characteristics. In group I, twenty animals up to 3rd lactation were incorporated, whereas thirty animals from 4th to 5th lactation were included in group II. The period when buffaloes showed teat engorgement was called as Doka period, is the local term used by the farmers in North India that can be taken as a visual sign for prediction of estrus. The length and diameter of teats, before and after milking were measured, for both the groups during morning and evening milking and subsequently analyzed. The present study confirmed that the teat diameter (mm) before milking during Doka was significantly (plessthan0.05) higher as compared to pre Doka period in both the groups (group-I - 37.01 ±2.13 vs 28.42 ±1.64, group-II - 53.49 ±2.82 vs 41.096±2.16). The teat diameter (mm) during Doka period after milking was significantly (plessthan0.05) more in both the groups (group-I - 26.65 ±1.26 vs 24.44 ±1.16, group-II - 39.27 ±2.41 vs 35.31 ±2.17) as compared to pre Doka period. So, it can be concluded that the changes in teat diameter can safely be considered as a sign of Doka and the forthcoming heat in Murrah buffaloes. However, the difference in teat length between different stages of Doka was found to be non significant.

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