scholarly journals Intestinal dysbiosis in women in early pregnancy

2019 ◽  
Vol 68 (5) ◽  
pp. 11-18
Author(s):  
Alexandr A. Bezmenko ◽  
Andrei A. Schmidt ◽  
Natalya D. Sadovaya
Keyword(s):  
Large Intestine ◽  
Normal Pregnancy ◽  
Main Group ◽  
Control Group ◽  
Microbiological Analysis ◽  
Normal Flora ◽  
High Concentration ◽  
Pathogenic Potential ◽  
Intestinal Dysbiosis ◽  
Threatened Abortion

Hypothesis/aims of study. To date, there have been some publications on the possible role of systemic endotoxinemia resulting from intestinal dysbiosis in the pathogenesis of miscarriage. The aim of this study was to compare the state of intestinal microbiocenosis and the level of endotoxinemia in women with threatened abortion and normal pregnancy. Study design, materials and methods. The study involved 50 women aged 18 to 35 years (mean age 28.1 0.6 years) in pregnancy from 6 to 18 weeks. The main group consisted of 30 women with threatened abortion. The control group included 20 women with normal pregnancy. Patients of the main and control groups were examined in accordance with the order of the Ministry of Health of the Russian Federation No. 572n. In addition, a qualitative and quantitative microbiological analysis of feces was performed using the real-time PCR method. The level of endotoxinemia was studied to assess the possible systemic effect of intestinal dysbacteriosis on a pregnant woman. Results. 23.3% of patients of the main group were diagnosed with intestinal dysbiosis of degree I, 60% of degree II, and 16.6% of degree III. In patients of the control group, dysbiotic changes corresponded mainly to degree I (75%) and degree II (25%). Degree III was not detected. Microbiological disorders in the main group were characterized by a decrease in the number of members of intestinal normal flora and a high concentration of opportunistic microorganisms. Microbiological disorders in the control group were characterized by a decrease in the intensity of colonization of the large intestine by lactobacilli and bifidobacteria. The average level of endotoxinemia in patients of the main group was 0.52 0.05 nmol/ml and was classified as elevated, significantly exceeding that in patients of the control group 0.34 0.05 nmol/ml. Conclusion. In patients with threatened abortion, intestinal microflora was characterized by a decrease in normal flora and a high concentration of opportunistic microorganisms with high a pathogenic potential and their associations. During normal pregnancy, microbiological disorders in the intestinal biocenosis were characterized by a decrease in the intensity of colonization of the large intestine by lactobacilli and bifidobacteria. The level of endotoxemia was directly proportional to the degree of dysbacteriosis of the intestine and was significantly higher in women of the main group.

Vaginal and intestinal microbiocenosis composition in pregnant women

2020 ◽  
Vol 68 (6) ◽  
pp. 29-36
Author(s):  
Alexandr A. Bezmenko ◽  
Natalya D. Sadovaya
Keyword(s):  
Pregnant Women ◽  
Normal Pregnancy ◽  
Main Group ◽  
Control Group ◽  
Microbiological Analysis ◽  
Control Groups ◽  
Threatened Miscarriage ◽  
Intestinal Dysbiosis ◽  
Grade Ii ◽  
And Control

Hypothesis/aims of study. It is assumed that intestinal dysbiosis, as well as bacterial vaginosis, may be one of the risk factors, and in some cases, the direct cause of pregnancy complications. This study was aimed to assess the intestinal and vaginal microbiocenosis composition in women with threatened miscarriage and normal pregnancy. Study design, materials and methods. The study involved 68 women aged 18 to 35 years (mean age 27.6 0.7 years) in pregnancy from 6 to 18 weeks. The main group consisted of 38 women with threatened miscarriage. The control group included women (n = 30) with normal pregnancy. Patients of the main and control groups were examined in accordance with the requirements of the Ministry of Health order No. 572n. In addition, a qualitative and quantitative microbiological analysis of vaginal discharge and feces was performed using the real-time polymerase chain reaction method. Results. Vaginal dysbiosis was detected in 60.5% of pregnant women of the main group and only 13.3% of women of the control group. In the main group, grade II dysbiosis was found in 23.7% of cases, and grade I dysbiosis in 36.8% of cases, while in the control group, these grades were found only in 3.3% and 10% of cases, respectively. Intestinal dysbiosis was diagnosed in 100% of the examined pregnant women. In patients of the main group, intestinal dysbiosis was detected as follows: grade I in 29% of cases, grade II in 52.6% of cases, and grade III in 18.4% of cases. In patients of the control group, the disorders corresponded mainly to grade I (83.4%) and, to a lesser extent, grade II (16.6%) dysbiosis, there being no cases of severe dysbiosis revealed. Conclusion. Women with threatened miscarriage had the most pronounced dysbiotic changes in vaginal and intestinal microflora compared to the control group. Vaginal dysbiosis was combined with intestinal dysbiosis in 100% of cases in patients of the main and control groups, and the degree of dysbiotic changes in the vaginal microflora was directly proportional to the degree of intestinal dysbiosis.

scholarly journals PREGNANCY AND OUTCOMES OF DELIVERY IN WOMEN WITH GENITAL ENDOMETRIOSIS

2020 ◽  
Vol 18 (5) ◽  
pp. 569-574
Author(s):  
M. A. Pavlovskaya ◽  
◽  
L. V. Gutikova ◽  
Y. V. Kukharchyk ◽  
◽  
...  
Keyword(s):  
Chronic Hypoxia ◽  
Clinical Laboratory ◽  
Normal Pregnancy ◽  
Main Group ◽  
Control Group ◽  
Fetal Growth Retardation ◽  
Perinatal Complications ◽  
Complicated Course ◽  
Previously Treated ◽  
Instrumental Methods

Background. The problem of genital endometriosis is relevant both in medical and in social aspects.Aim of the research. To analyze the course of pregnancy, childbirth and their outcomes in genital endometriosis to justify the critical terms of the complicated course of pregnancy and determine approaches for the development of therapeutic and preventive measures.Material and methods. The main group – 160 pregnant women, previously treated for genital endometriosis. The control group was 50 healthy women with a normal pregnancy. The generally accepted clinical laboratory and instrumental methods of the research were used. Statistical analysis of the data was carried out using the software package Statistica 10.0. Results. Gestational periods of 6-12 weeks, 18-22 weeks, 30-34 weeks of pregnancy are critical for women of the main group. The course of pregnancy is complicated by the threat of an early miscarriage (50%), placental disorders (65%), chronic hypoxia (35%) and fetal growth retardation (20%), anomalies of labor (60%).Conclusions. The complicated course of pregnancy in patients with genital endometriosis justifies the need to develop a comprehensive program for the prevention of gestational and perinatal complications.

Cytokine profile in women with threatened miscarriage and intestinal dysbiosis

2021 ◽  
Vol 70 (1) ◽  
pp. 51-58
Author(s):  
Alexander A. Bezmenko ◽  
Natalia D. Sadovaya ◽  
Marina E. Meshkova ◽  
Elena A. Malakhova
Keyword(s):  
Pregnant Women ◽  
Intestinal Microflora ◽  
Main Group ◽  
Control Group ◽  
Endotoxin Level ◽  
Threatened Miscarriage ◽  
Intestinal Dysbiosis ◽  
Grade Ii ◽  
Pro Inflammatory Cytokines ◽  
Intestinal Microbiocenosis

Hypothesis/Aims of study: Systemic endotoxinemia resulting from intestinal dysbiosis activates the Th1 immune response and excessive production of proinflammatory cytokines, which can cause abortion. This study was aimed at assessing interleukin levels in women with the threat of miscarriage and exploring their dependence on intestinal microbiocenosis and the level of endotoxinemia. Study design, materials and methods: The study involved 87 women aged 18 to 43 years in pregnancy from six to 22 weeks. The main group consisted of 50 women with a threatened miscarriage. The control group included 37 women with normal pregnancy. A qualitative and quantitative analysis of intestinal microbiocenosis was performed by real-time PCR, with endotoxin and interleukin levels evaluated using conventional methods. Results: In patients of the main group, intestinal dysbiosis of grade I was detected in 30 % (n = 15), of grade II in 46 % (n = 23), and of grade III in 24 % (n = 12) of cases. In the control group, intestinal microflora disorders corresponded to grade I dysbiosis in 67.6 % (n = 25) and grade II dysbiosis in 32.4 % (n = 12) of cases, there being no cases of severe dysbiosis revealed. The level of endotoxin in pregnant women of the main group was 0.57 0.02 nmol / ml and was classified as increased. The endotoxin level in the blood serum of pregnant women of the control group was 0.34 0.02 nmol / ml and was characterized as low (p 0.001). A strong correlation was found (r = 0.8, p 0.001) between the grade of intestinal dysbiosis and the level of endotoxinemia. The cytokine profile in patients of the main group was characterized by increased concentrations of pro-inflammatory cytokines (IL-1 4.9 1.6 pg / ml, IL-6 4.8 1.5 pg / ml) and a decreased concentration of anti-inflammatory cytokine (IL-10 18.0 4.5 pg / ml), when compared to the control group (IL-1 1.8 0.2 pg / ml, IL-6 2.1 0.2 pg / ml, IL-10 30.3 4.4 pg/ml). There were a statistically significant moderate positive correlation between the level of endotoxinemia and the concentration of pro-inflammatory cytokines and a weak negative correlation between the endotoxin level and the concentration of anti-inflammatory IL-10. Conclusion: Endotoxinemia, which occurs as a result of intestinal microflora dysbiosis and activates pro-inflammatory pathways, can really be as a triggering factor in the pathogenesis of miscarriage in pregnant women.

scholarly journals Role of cytohormonal study in normal pregnancy and in threatened abortion

2018 ◽  
Vol 7 (5) ◽  
pp. 1735
Author(s):  
Shailesh B. Patil ◽  
Milind B. Patil
Keyword(s):  
Normal Pregnancy ◽  
Fetal Outcome ◽  
Control Group ◽  
Vaginal Smear ◽  
Age Group ◽  
Number Of Patients ◽  
Significant Difference ◽  
Parity Number ◽  
Threatened Abortion

Background: It is certain from clinical experience of many that one or more hemorrhages in early pregnancy can still end up in good fetal outcome. So, our study deals with comparison of cytohormonal study in pregnancy and threatened abortion. The study was conducted with the aim of utilizing colpocytogram as a tool in assessing and treating cases of threatened abortion and comparing them with normal pregnant women.Methods: Patients attending antenatal care unit on outdoor basis and labelled as normal pregnancy cases were considered as control group. The patients of threatened abortion were studied when they were admitted in Gynecology department for indoor treatment. Patients were studied taking into consideration their age, parity, number of abortions, complaints (P/V bleeding, pain in abdomen), gestational age, per abdomen and per vaginal findings and also USG findings and vaginal smear pattern.Results: Maximum number of patients was present in the age group of 21-25 years in both the groups. While only 13.33% had normal smear pattern in threatened abortion group. 86.67% patients in threatened abortion group showed abnormal smear pattern. There is statistically significant difference was found (p<0.05). There is statistically significant was found (P<0.01) and indicates good effects of the drug on the vaginal epithelium.Conclusions: The cytohormonal study acts as a simple, reliable, good, noninvasive method for evaluation of hormonal pattern in normal pregnancy and threatened abortion. 

scholarly journals Serum Malondialdehyde Level as a Risk Factor for Threatened Abortion

2016 ◽  
Author(s):  
Gusti N Sutama ◽  
I Gede P Surya
Keyword(s):  
Risk Factor ◽  
Serum Levels ◽  
Normal Pregnancy ◽  
T Test ◽  
Control Group ◽  
Chi Square ◽  
Chi Square Test ◽  
Threatened Abortion ◽  
Average Serum ◽  
High Level

Objective: To determine the role of serum malondialdehyde level as a risk factor for threatened abortion. Method: Our study was a case‐control study. We examined 60 pregnant women as our subject, 30 subjects with threatened abortion as cases and 30 subjects with normal pregnancy as control group. Serum levels of malondialdehyde of each woman was examined in the Biochemistry Laboratory Faculty of Medicine Gadjah Mada University in Yogyakarta. We tested the normality of our data using Kolmogorov‐ Smirnov test, and analysis was then performed using independent sample t‐test. To determine the relationship of serum malondialdehyde with threatened abortion, Chi‐Square test was used. Result: From this study we found that the average serum malondialdehyde level in threatened abortion was 1.33K0.11 nmol/ml, while the average level of serum malondialdehyde in normal pregnancy was 1.03K0.10 nmol/ml. The analysis using independent t‐test shows that the average serum malondialdehyde level on the two groups was significantly different (p=0.001). Based on the cut‐off value of 1.12 nmol/ml, we found that the relative risk of threatened abortion is 29.57 times (95% CI=6.85‐127.64, p=0.001) in those with a high level of serum malondialdehyde. Conclusion: The serum malondialdehyde level in threatened abortion was significantly different compared to normal pregnancy. A high level of serum malondialdehyde in pregnancy was a risk factor for threatened abortion. Keywords: malondialdehyde, normal pregnancy, threatened abortion

scholarly journals The efficiency of treatment of vaginal infections in women with a history of miscarriage

2019 ◽  
Vol 68 (5) ◽  
pp. 63-74
Author(s):  
Anna A. Siniakova ◽  
Elena V. Shipitsyna ◽  
Olga V. Budilovskaya ◽  
Vyacheslav M. Bolotskikh ◽  
Alevtina M. Savicheva
Keyword(s):  
Bacterial Vaginosis ◽  
Pregnant Women ◽  
First Trimester ◽  
Main Group ◽  
Control Group ◽  
Vaginal Infections ◽  
Pregnancy And Childbirth ◽  
Vaginal Microflora ◽  
History Of ◽  
Threatened Abortion

Hypothesis/aims of study. The problem of vaginal infections during pregnancy is of high importance in obstetric practice. To predict the risks and reduce the frequency of pregnancy and childbirth complications, it is necessary to dynamically assess the vaginal microflora and treat its disorders. The aim of the study was to investigate the vaginal microflora and evaluate the effectiveness of treating vaginal infections in pregnant women with a history of miscarriage. Study design, materials and methods. The study included 153 pregnant women in the first trimester. The main group (group I) consisted of 99 women with a history of miscarriage, 35 of whom had signs of threatened abortion (subgroup IA) and 64 did not (subgroup IB). The control group (group II) comprised 54 women without a history of miscarriage and signs of threatened abortion. The vaginal microflora was examined using microscopic, bacteriological and quantitative real-time PCR methods. All patients with an established vaginal infection (bacterial vaginosis, aerobic vaginitis, and vulvovaginal candidiasis) received etiotropic therapy, depending on the microorganisms identified and their sensitivity to antimicrobial drugs. After treatment, in order to assess the effectiveness of the therapy, the vaginal microflora was examined in the second trimester and the outcomes and complications of present pregnancy were evaluated. Results. In women of subgroup IA, vulvovaginitis and bacterial vaginosis were detected 3.5 times more often compared to the control group, and 1.6 times more often compared to subgroup IB (66% and 19%, respectively, p 0.001; 66% and 42%, respectively, p 0.05). Aerobic vaginitis was the most frequent vaginal infection in the first trimester of pregnancy in women of the main group (p 0.05). After treatment, the frequency of the vaginal infections in the second trimester in women of the main group significantly decreased: by 1.9 times in subgroup IA and by 1.5 times in subgroup IB (p 0.05). There were no significant differences in the frequency of adverse pregnancy outcomes in women with bacterial vaginosis or vulvovaginitis as compared to women with normal vaginal microflora. Nevertheless, pregnancy and childbirth complications were diagnosed 4 times more frequently in the main group (23% and 6%, respectively, p 0.05), with the complications occurring significantly more often in the cases of vulvovaginitis or bacterial vaginosis and signs of threatened abortion in the first trimester (p 0.05). Conclusion. Etiotropic therapy of vaginal infections diagnosed in the first trimester of pregnancy in women with a history of miscarriage was highly effective. In 40% of women, vaginal microbiocenosis normalized, and the clinical symptoms of vaginosis/vaginitis disappeared. Differences in the frequency of adverse pregnancy outcomes in women with vulvovaginitis or bacterial vaginosis in the first trimester and in women with normal vaginal microbiocenosis were not significant. However, the treatment of vaginal infections in the group of pregnant women with a history of miscarriage did not significantly affect the frequency of pregnancy and childbirth complications.

scholarly journals Intestinal mucosal lymphocytes in neonatal sepsis

2019 ◽  
Vol 64 (4) ◽  
pp. 52-59
Author(s):  
Kh. S. Khaertynov ◽  
V. A. Anokhin ◽  
G. R. Burganova ◽  
G. O. Pevnev ◽  
M. O. Mavlikeev ◽  
...  
Keyword(s):  
Mucous Membrane ◽  
Large Intestine ◽  
Intestinal Mucosa ◽  
Neonatal Sepsis ◽  
Morphological Changes ◽  
Intestinal Bacteria ◽  
Main Group ◽  
Control Group ◽  
Small Intestinal Mucosa ◽  
Cd4 Lymphocytes

We studied the autopsy material obtained from 7 children who died in the neonatal period in order to evaluate the composition of lymphocytes of the intestinal mucosa against the background of morphological changes in the tissues of the gastrointestinal tract in newborns with sepsis. The main group consisted of 4 children with neonatal sepsis, the control group – of 3 newborns who died from other causes. The research material included the specimen of the small and large intestine.Results. Small intestine: it was found that there were less CD4 + lymphocytes in the small intestinal mucosa in the group of children who died from neonatal sepsis in 75% of cases than in the control group, but this difference was not statistically significant (p=0.1). There were no differences in the number of CD8 + and CD20 + cells in the studied groups. Large intestine: the number of CD4 + lymphocytes of the mucous membrane of the colon was greater in the main group of children than in the control group (p=0.03). An increase in the number of CD4 + cells was registered in 3 of 4 cases of neonatal sepsis. The number of CD8+ and CD20+ lymphocytes in the studied groups was the same (р>0.05).Conclusion. The increase in T-lymphocytes CD4+ in the mucous membrane of the large intestine is probably connected with the antigenic stimulation of opportunistic intestinal bacteria. We found no morphological signs of the suppression of the cells of adaptive immunity associated with the intestinal mucosa. 

EVALUATION OF MICROBIOCENOSIS OF LARGE INTESTINE AND INTESTINAL PERMEABILITY IN OBESE ADOLESCENTS

2021 ◽  
pp. 23-26
Author(s):  
M. A. Khomenko ◽  
T. P. Osolodchenko
Keyword(s):  
Intestinal Permeability ◽  
Large Intestine ◽  
Interleukin 6 ◽  
Interleukin 10 ◽  
The Body ◽  
Control Group ◽  
Alcoholic Fatty Liver ◽  
Obese Adolescents ◽  
Low Intensity ◽  
Intestinal Dysbiosis

Obesity is one of the most common non−infectious diseases worldwide among both adults and children. It is associated with the development of diseases such as metabolic syndrome, type 2 diabetes, non−alcoholic fatty liver disease, cardiovascular disease etc. The mechanisms proposed to explain the development and progression of obesity include chronic low−intensity inflammation, bacterial translocation, and endotoxemia, which may resulted from dysbiosis and increased intestinal permeability. To study anthropometric parameters, levels of zonulin, lipopolysaccharide, interleukin−6 and interleukin−10, indices of the colon microbiota, 74 adolescents with obesity aged 12−17 years were examined. The correlation analysis of anthropometric and laboratory indices, between anthropometric ones and those of microflora of a large intestine depending on sex was performed. It is noted that obesity is accompanied by the formation of intestinal dysbiosis in 78.2 % of patients with a decrease in the obligate microflora and an increase in the conditionally pathogenic microflora. In adolescent patients, a significant rise in interleukin−6 levels and a tendency to increase interleukin−10 levels compared with adolescents with normal weight, which is a sign of low−intensity inflammation. There was a significant increase in zonulin levels in obese adolescents compared with those in the control group, that may be an evidence of increased intestinal permeability. Positive correlations have been reported between the body weight, abdominal fat distribution, and increased intestinal permeability as well as activation of low−intensity inflammation. In obese adolescents, in the presence of dysbiotic disorders, it is advisable to harmonize the diet and style and correct intestinal dysbiosis with the intestinal barrier restoration of. Key words: zonulin, lipopolysaccharide, interleukins, microbiota, adolescents, obesity.

scholarly journals SPECIALTIES OF HYSTOMORPHOMETRICAL CHANGES IN PLACENTA OF WOMEN WITH EARLY AND LATE PREECLAMPSIA

2020 ◽  
Vol 73 (1) ◽  
pp. 151-155 ◽  
Author(s):  
Dmitro G. Konkov ◽  
Alina O. Piskun ◽  
Oksana A. Taran ◽  
Galyna V. Kostur
Keyword(s):  
Specific Gravity ◽  
Blood Vessels ◽  
Structural Transformation ◽  
Normal Pregnancy ◽  
Blood Stream ◽  
Main Group ◽  
Control Group ◽  
Early Form ◽  
Perinatal Pathology ◽  
Placental Blood

The aim: To find out typical pathomorphological differences in placenta of women with early and late preeclampsia. Materials and methods: Investigation includes 40 placentas from deliveries in women with preeclampsia (main group) and 40 placentas from physiological delivery in somatically healthy women, who had no complications during pregnancy (control group). Placentas in the main group were devided into two sub-groups (20 in each) – with early and late preeclampsia. Specialties of the blood vessels in normal pregnancy were investigated, and their structural transformation with the developement of preeclampsia, according to the appearence of perinatal pathology. Morphometrical data of the blood stream was investigated with the help of eyepiece and program Image Tools 3,6. Results: Significant decrease of weight (p<0,05), square and volume of placenta was common to early preeclampsia, comparing to the same characteristics in late Preeclampsia (PE). Specific gravity of villi without vessels, hardened blood vessels, hardened villi and fibrinoid altered vessels was increased statistically significantly (p<0,05) in placenta of women with early PE, comparing to women with late PE. The number of effective blood vessels crossings was determined mostly in late PE, comparing to the early form (p<0,05). Found out significant defferences (p<0,05) in changes of hystovasoarchitecture of placenta in early preeclampsia, according to the number of immature villi and villi with no signs of compensatory angiomatosis. Conclusions: Increased number of hypoplasia of placenta, breach of effective placental blood stream and significant decrease of compensatory and adaptive changes in placenta are more common to early PE, comparing to late PE.

scholarly journals SURGICAL CORRECTION OF THE SYNDROME OF ENTERAL INSUFFICIENCY AT PATIENTS WITH ABDOMINAL SEPSIS

2020 ◽  
pp. 16-20
Author(s):  
Yu. M. Solovey ◽  
V. P. Polovuy ◽  
M. M. Solovey
Keyword(s):  
Surgical Treatment ◽  
Large Intestine ◽  
Abdominal Cavity ◽  
Abdominal Sepsis ◽  
Main Group ◽  
Oxidative Modification ◽  
Control Group ◽  
Primary Group ◽  
Complex Treatment ◽  
Small And Large Intestine

Summary. The analysis of the treatment of 38 patients with abdominal sepsis. In the surgical treatment of patients of the main group on abdominal sepsis the main group used its own developed method of total decompression, rehabilitation and electrostimulation of the small and large intestine by applying two dual channel probes (one with a larger diameter for aspiration of intestinal contents (decompression), another smaller one for the implementation of the reorganization and the introduction of drugs, with copper conductors, spirally placed between a thick and a thin channel diameter of 2 mm, for electrostimulation. Results of the study and their discussion. Evaluated the effectiveness of the developed method of correction of a syndrome of enteral nedostatocne in complex treatment of patients with abdominal sepsis. After the initial operation is set to increase in clearance of reorganization in the primary group to (91,3±4,8) % vs (83,5±3,4) % in the control group (p<0.05). application of the developed method of correction of a syndrome of enteral insufficiency in the complex treatment of abdominal sepsis leads to a decrease in the concentration of products of lipid peroxidation, the degree of oxidative modification of proteins and helps to preserve antioxidant enzymes activity in the process flow Conclusions: the Use in the surgical treatment of patients with abdominal sepsis developed method of total decompression, Sanz and electrostimulation of the small and large intestine after the first operation allows to reduce microbial contamination of the abdominal cavity to (91,3±4,8) % vs (83,5±3,4) % in the control group (p<0.05).

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