scholarly journals HYPOTHERMIC EFFECT OF ANTIHYPOXANTS πQ1983 AND πQ2170

2012 ◽  
Vol 10 (4) ◽  
pp. 78-82
Author(s):  
Denis Vladimirovich Sosin ◽  
Andrey Viktorovich Yevseyev ◽  
Edgar Andreyevich Parfenov ◽  
Vitaliy Andreyevich Pravdivtsev ◽  
Marina Anatolyevna Yevseyeva ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Metal Complex ◽  
Rectal Temperature ◽  
Death Rate ◽  
Temperature Level ◽  
Hypothermic Effect ◽  
Dose Dependent

In experiments on mice the influence of the new antihypoxants (selenium containing metal complex substances πQ1983, πQ2170) on rectal temperature level dynamic after parenteral and enteral introductions was studied. It is established that both substances perform clear dose-dependent hypothermic effect exceeding an action of the antihypoxant amthizole. Was supposed that substance πQ2170 has high acute toxicity affirmed by significant mice death-rate.

scholarly journals Testing of new selenium containing metal complex compounds by acute hypoxia-hypercapnia method

2017 ◽  
Vol 15 (4) ◽  
pp. 46-52
Author(s):  
Andrey V Evseev ◽  
Dmitrij V Surmenev ◽  
Edgar A Parfenov ◽  
Marina A Evseeva ◽  
Denis V Sosin ◽  
...  
Keyword(s):  
Metal Complex ◽  
Life Span ◽  
Rectal Temperature ◽  
Acute Hypoxia ◽  
Complex Compounds ◽  
Antihypoxic Activity ◽  
Positive Interaction ◽  
Antihypoxic Effect ◽  
The Right ◽  
Dose Dependent

Aim. To test the new selenium containing metal complex (Zn2+) compounds by acute hypoxia-hypercapnia method in experiments on mice for antihypoxic effect finding. Methods. The screening of antihypoxants in experiments on mice placed in acute hypoxia-hypercapnia condition (AH + H) among 11 new metal complex selenium containing compounds have been performed. Condition of AH + H in animals was formed by their placing in hermetic glass boxes with 0.25 L free volume. Each substance has been used once by intraperitoneal introduction 60 min. before mice placing in AH + H area in doses 10, 25, and 50 mg/kg. The “life span” parameter was used as a criterion of antihypoxic activity. At the definite steps of investigation all animals where undergone to rectal temperature measuring. Results. The antihypoxic effect was shown by 4 compounds among 11 (πQ2719, πQ2720, πQ2721, πQ2882). The greatest result in life span was found in substance πQ2721. In this case the right positive interaction between doses of substances and results was marked - doses 10, 25 and 50 mg/kg provide life span enlargements to 25.1, 81.7 (р < 0.05), and 186.1% (р < 0.005) in comparison with control groups correspondently. The efficiency of etalon substance πQ1983 was near to that of πQ2721 but in dose 50 mg/kg it becomes weaker in activity than new compound. Conclusion. The test resultant of 11 new metal complex (Zn2+) substance with selenium in structure of ligand in use of acute hypoxia-hypercapnia method shows the πQ2721 as an antihypoxant that was confirmed by significant mice life span enlargement after doses 25 and 50 mg/kg during its action that according with dose-dependent rectal temperature decrease. (For citation: Yevseyev AV, Surmenev DV, Parfenov EA, et al. Testing of new selenium containing metal complex compounds by acute hypoxia-hypercapnia method. Reviews on Clinical Pharmacology and Drug Therapy. 2017;15(4):46-52. doi: 10.17816/RCF15446-52).

scholarly journals Tin thiocarbonohydrazone complexes: synthesis, crystal structures and biological evaluation

2019 ◽  
Vol 8 (6) ◽  
pp. 862-867 ◽  
Author(s):  
Jin Wang ◽  
Yu-Ting Wang ◽  
Yan Fang ◽  
Yan-Li Lu ◽  
Ming-Xue Li
Keyword(s):  
Hepatocellular Carcinoma ◽  
Acute Toxicity ◽  
Hepg2 Cells ◽  
Biological Evaluation ◽  
Cytotoxic Potential ◽  
Dependent Effect ◽  
Ic50 Value ◽  
Ld50 Value ◽  
Dose Dependent ◽  
Dose Dependent Effect

Abstract In this article, three organotin complexes formulated as [(Me)2Sn(H2L1)] (1), [(Ph)2Sn(H2L1)]·MeOH (2) and [(Me)2Sn(HL2)(OAc)]4(Me)2O (3) (H4L1 = bis(2-hydroxybenzaldehyde) thiocarbohydrazone and H2L2 = bis(2-acetylpyrazine) thiocarbonohydrazone) have been synthesized and structurally characterized. Growth inhibition assays indicated that both the proligands and the three complexes are capable of showing anticancer activity against the human hepatocellular carcinoma HepG2 cells with H2L2 and complex 3 showing much higher cytotoxic potential. Subsequent toxicity studies on normal QSG7701cells showed that complex 3 has the highest tumor cell selectivity, and its IC50 value on QSG7701 cells is 8.48 fold higher than that in HepG2 cells. In acute toxicity experiments, complex 3 produces a dose-dependent effect in NIH mice with a LD50 value of 17.2 mg kg−1.

scholarly journals Pharmacognostic assessment and pre-clinical toxicity of ethanolic extract from Aspidosperma subincanum Mart. stem bark (Guatambu)

2021 ◽  
Vol 10 (9) ◽  
pp. e17510917547
Author(s):  
Nilda Maria Alves ◽  
Marina Alves Coelho Silva ◽  
Leila Maria Leal Parente ◽  
José Realino de Paula ◽  
Alessandro de Carvalho Cruz ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Ethanolic Extract ◽  
Stem Bark ◽  
World Population ◽  
Subacute Toxicity ◽  
Short Period ◽  
Treatment Objective ◽  
Open Markets ◽  
Dose Dependent ◽  
Vegetal Species

Ethnopharmacological relevance: Diabetes mellitus is a disease that affects approximately 10% of the world population. As it is a chronic disease, several patients search for alternatives to the traditional allopathic treatment, such as medicinal plants. In Brazil, the Aspidosperma subincanum (Guatambu) is one of the vegetal species commercialized in open markets used for diabetes popular treatment. Objective: To perform the phytochemical prospection and pre-clinical assessment of the ethanolic extract from stem bark of A. subincanum bark (EEAS). Material and methods: A. subincanum stem barks were collected in state of Goiás, Brazil and the EEAS was obtained by cold maceration. The phytochemical prospection was performed and acute and subacute toxicity assessments were performed according to OECD guidelines. Results: Phytochemical prospection identified the presence of steroids, triterpenes, saponins, tannins, alkaloids, coumarins, and resins. The acute toxicity showed no death in EEAS doses administered intraperitoneally (500, 1000, and 2000 mg/kg) and orally (5000 mg/kg). The subacute toxicity showed signs of nephrotoxicity, hepatotoxicity, and lung damages in the two highest doses tested (250, 250 mg/kg) in a dose-dependent way. However, the satellite group showed reversion of the lesions after 30 days of EEAS interruption. Conclusion: The acute toxicity of EEAS suggests an LD50 > 5000 mg/kg. The oral use of EEAS during a short period (30 days) or more can be hazardous at doses similar to that tested in this study and may represent a risk for those who consume this kind of extract as a medicinal plant.

scholarly journals Acute and sub-acute toxicity study of the root extracts of Fagaropsis hildebrandtii in mice and evaluation of their antimicrobial effects

F1000Research ◽  
2020 ◽  
Vol 8 ◽  
pp. 1444
Author(s):  
Beatrice Mwende Muia ◽  
James Mucunu Mbaria ◽  
Laetitia Wakonyu Kanja ◽  
Nduhiu Gitahi ◽  
Paul Onyango Okumu ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Oral Toxicity ◽  
Staph Aureus ◽  
Antimicrobial Effects ◽  
Root Extracts ◽  
Bactericidal Effects ◽  
Microbial Infections ◽  
Kidney Liver ◽  
Future Work ◽  
Dose Dependent

Background: Among the Kamba community of Kenya, roots of Fagaropsis hildebrandtii (FH) are boiled and used in managing cough, fertility problems, and microbial infections. The safety of this plant in oral administration and the validity of the ethnomedical claims are unverified. This study evaluated the toxicity of the aqueous and hexane root extracts of FH in mice and antimicrobial effects against Staphylococcus aureus, Salmonella typhimurium and Candida albicans. Methods: Doses (300 and 2000 mg/kg) of the extracts were administered orally to mice for 14 days. The weight, feed, and water consumption, organ weight of mice and gross macroscopy of the liver were used in evaluating acute toxicity. Mice were additionally treated with 250, 500, and 1000 mg/kg body-weight doses of the extracts for 28 days and haematological, biochemical, and histological parameters noted. The minimum inhibitory and minimum bactericidal/fungicidal concentrations (MIC; MBC/MFC) of the extracts against the aforementioned pathogens were determined by broth dilution. Results: Acute oral toxicity of the extracts was >2000 mg/kg, there were dose dependent changes in haematological and biochemical parameters, all female mice died when treated with doses of 1000mg/kg and doses ≥500 mg/kg caused tubular degeneration and haemorrhage of the kidney, cloudy swelling of hepatocytes, and multifocal necrosis and pyknosis in the liver. The MBC/MIC ratio of each of the extracts against Staph. aureus and S. typhimurium was 2, while C. albicans was not sensitive to any of the extracts. Conclusions: Long term use of FH root extracts was associated with dose-dependent changes in the mice kidney, liver and in biochemical and haematological parameters. Root extracts of FH are bactericidal against Staph. aureus and S. typhimurium but have no effect on C. albicans. Future work should aim at identifying the metabolites responsible for the observed toxic and bactericidal effects of the FH root extracts.

scholarly journals Phytopharmacological Evaluation of Alcoholic Extract of Berberis aristata Leaf in the Treatment of Gastric Ulcer

2020 ◽  
Vol 13 (1) ◽  
pp. 1-5
Author(s):  
Ratnaker Singh ◽  
Y. Trilochana
Keyword(s):  
Acute Toxicity ◽  
Crude Extract ◽  
Antioxidant Activities ◽  
Ethanolic Extract ◽  
Total Phenolic ◽  
Antiulcer Activity ◽  
Alcoholic Extract ◽  
Dose Dependent

For over a century, peptic ulcer has been one of the most common gastrointestinal tract (GIT) disorder. There are number of drugs are now available for treatment. Drugs of herbal origin reduce the offensive factors and have proved to be safe, clinically effective, relatively less expensive, globally competitive, and with better patient tolerance.This study was performed to assess the anti-ulcer activity on different parts of B.aristata. Apart from that, acute toxicity, qualitative chemical analysis, total phenolic content (TPC), total flavonoid content(TFC) and in vitro antioxidant activities were evaluated. The potentially active plant part was selected for screening as gastro protective, in vivo antioxidant and antisecretory activities in ulcerated rats.The 50% ethanolic extract of B. aristata were subjected to preliminary phytochemical screening, estimation of TFC and TPC. The crude extract from the leaves of B. aristata gave best antiulcer activity among flower and stem. In acute toxicity studies, the administration of the crude extract of B. aristata leaves did not reveal any adverse effects or toxicity in rats at fourteen days observations.The results of these studies have shown that ethylexract of B.aristata leaf (EEBAL) produced a significant dose dependent ulcerprotective, antioxidant and antisecretory activity by blocking the activity of proton pump, protecting from antioxidants produced during stress induced ulcer and by enhancing glycoprotein levels.

scholarly journals The effect of adding herbal extracts to drinking water on body temperature, level of thyroid hormones and H:L ratio in the blood of broiler chickens exposed to elevated ambient temperature

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Iwona Skomorucha ◽  
Ewa Sosnówka-Czajka
Keyword(s):  
Drinking Water ◽  
Body Temperature ◽  
Thyroid Hormones ◽  
Ambient Temperature ◽  
Rectal Temperature ◽  
Broiler Chickens ◽  
Herbal Extracts ◽  
Temperature Level ◽  
Lemon Balm ◽  
Group I

Abstract The aim of the study was to determine the effect of supplementing drinking water with extracts from lemon balm, sage and nettle on body temperature, level of thyroid hormones and the heterophil to lymphocyte (H:L) ratio in the blood of broiler chickens exposed to elevated rearing temperature. One-day-old Ross 308 chicks were divided into four groups: group I (CON) and experimental groups II (LB), III (S) and IV (N), in which, from 22 to 42 days of rearing, drinkers were supplemented with lemon balm extract, sage extract or nettle extract (2 ml/l water), respectively. In addition, at 5 weeks of growth, all the groups were exposed to elevated ambient temperature (up to 30°C) for 5 days, after which the recommended thermal conditions were reinstated. During the study, mortality, rectal temperature and radiated temperature of the broilers were monitored. Blood was collected from 10 birds per group to determine the concentration of thyroid hormones (T3 and T4) and to make blood smears. The H:L ratio was determined based on the percentage of heterophils (H) and lymphocytes (L). The herbal extracts from lemon balm, sage and nettle, added to drinking water at 2 ml/l, reduced the blood level of the thyroid hormone (triiodothyronine) and rectal temperature in the experimental broilers during the initial period of thermal stress. Chickens receiving the nettle extract were also characterised by lower radiated temperature of the unfeathered body, a lower H:L ratio in the blood during the increase in ambient temperature, and the lowest mortality percentage. It can therefore be considered that the dietary supplementation of nettle, in the form and concentration used in this study, had the most favourable effect on the physiological status of the birds (body temperature, level of thyroid hormones and H:L ratio) ratio exposed to elevated ambient temperatures (30oC), and thus on their welfare.

Anticancer activity, phytochemical screening and acute toxicity evaluation of an aqueous extract of Aristolochia longa L.

2017 ◽  
Vol 6 (1) ◽  
pp. 20 ◽  
Author(s):  
Bachir Benarba ◽  
Atanasio Pandiella ◽  
Almahy Elmallah
Keyword(s):  
Breast Cancer ◽  
Acute Toxicity ◽  
Cell Lines ◽  
Aqueous Extract ◽  
Flavonoid Glycosides ◽  
Dependent Manner ◽  
Oral Toxicity ◽  
Cytotoxic Effects ◽  
Tlc Analysis ◽  
Dose Dependent

Aristolochia longa (Aristolochiaceae) is widely used in traditional medicine. The present study was carried out to investigate the cytotoxic activity and the acute toxicity of an aqueous extract of A. longa roots. Also, the phytochemical composition of the extract was evaluated. The cytotoxic effects of the aqueous extract in triple negative breast cancer MDA-MB-231 and HBL-100 cell lines was evaluated by MTT assay. A. longa roots were screened for the presence of phytochemical constituents using the standard qualitative phytochemical procedures. The acute oral toxicity (5000 mg/kg limited dose test) was evaluated. Our results showed that both cells were inhibited in a dose-dependent manner by A. longa aqueous extract. The IC50 of A. longa aqueous extract was estimated after 72h treatment at 40μg/ml and 97μg/ml in HBL100 and MDA-MB-231 cell lines, respectively. A. longa aqueous extract at a concentration of 500μg/ml suppressed effectively the cell growth of HBL100 and MDA-MB-231 cells. TLC analysis revealed the presence of flavonols, flavones and/or flavonoid glycosides as major compounds in the extract. Results of the acute toxicity study suggest the non-toxicity of the A. longa aqueous extract to the liver. Interestingly, the renal function was not affected by the extract administration at 5000mg/kg. A. longa aqueous extract could be toxicologically safe when administered orally in rats in a single dose. A. longa could be considered as a promising and safe source for developing novel therapeutics against breast cancer. Keywords: Aristolochia longa, breast cancer, phytochemical, acute toxicity, TLC.

Dose-Dependent Vehicle Differences in the Acute Toxicity of Bromodichloromethane

1994 ◽  
Vol 23 (1) ◽  
pp. 132-140 ◽  
Author(s):  
PATRICK D. LILLY ◽  
JANE ELLEN SIMMONS ◽  
REX A. PEGRAM
Keyword(s):  
Acute Toxicity ◽  
Dose Dependent

scholarly journals Antinociceptive Effect of the Ethanol Leaf Extract of Balanites aegyptiaca Linn in Albino Rats

2020 ◽  
Vol 27 (2) ◽  
pp. 39-46
Author(s):  
O.A. Sodipo ◽  
J. Yakubu ◽  
A.M. Dungus ◽  
B. Wampana
Keyword(s):  
Acute Toxicity ◽  
Chemical Constituents ◽  
Antinociceptive Effect ◽  
Ethanol Extract ◽  
Albino Rats ◽  
Balanites Aegyptiaca ◽  
Hot Plate ◽  
Tail Immersion ◽  
Anti Inflammatory ◽  
Dose Dependent

Balanites aegyptiaca is a medicinal plant that has been used in a variety of folk medicines in India and Africa for the treatment of different ailments such as syphilis, jaundice, liver and spleen problems, epilepsy, yellow fever. This research aimed at investigating the phyt ochemical constituents, acute toxicity, anti inflammatory and antinociceptive activities of the ethanol extract of Balanites aegyptiaca (EEBA) leaves. The plant material was extracted using ethanol as solvent. The dried ethanol extract was screened for the presence of phytochemicals; Acute toxicity (LD50) was  determined using Lorke’s method while anti inflammatory and antinociceptive activities were evaluated using hot plate and tail immersion methods in albino rats. Results of the study revealed that phyto chemical constituents such as flavonoids, carbohydrates, saponins and glycosides were found in the EEBA, intraperitoneal LD 50 of 2154 mg/kg shows the substance is non toxic. The EEBA produced significant (p <0.05) and dose dependent anti inflammatory and a ntinociceptive activities at all test doses (200, 400 and 600 mg/kg). The presence of the phytochemicals detected might be responsible for the demonstrated anti inflammatory and antinociceptive activities in the plant extract. Keywords: Phytochemical, Anti inflammatory, Antinociceptive , Balanites aegyptiaca

Sign in / Sign up

Export Citation Format

Share Document