scholarly journals Contribution to the Ethnobotanical, Phytochemical, Antimicrobial and Antioxidant Study of the Leaves’ Aqueous Extract of the Common Walnut "Juglans regia L."

Author(s):  
Amel Bennacer ◽  
Hamida Saida Cherif

This work has focused on phytochemical screening of the Common WalnutJuglans regiaL. leaves, and biological tests: antioxidant and antimicrobial effects of the aqueous extracts (infusion, crude) of the common walnuts leaves that we’ve picked, dried and preserved. An ethnobotanical survey in Blida region has been achieved, where it has been concluded that the inhabitants of this region use the common walnuts leaves in their daily care, and the various organs of the Common walnut are widely used as well. The phytochemical screening based on specific tests helped to highlight the presence of the main secondary metabolites such as tannins, flavonoids, alkaloids, free quinones, anthocyanins and saponins, which could be responsible for the biological properties. The antimicrobial study was conducted on bacterial strains and a yeast showed that both aqueous (infusion, crude) extracts have antibacterial effect against certain bacterial strains:Staphylococcus aureus(40.43 mm),Staphylococcus epidermidis(35.36 mm),Sarcina lutea(31, 17 mm), respectively, and an inhibitory effect only for the crude aqueous extract ofEschirichia coli(12 mm),Klebsiella pneumoniae(12 mm), for against these two extracts were proved to be inactive forCandida albicans.In addition, the study of antioxidant activity by the method of reduction of iron (FRAP) and the method of reduction of free radical (DPPH) has revealed an average rate of aqueous extract (infusion) compared with that of ascorbic acid and rutin respectively. With an EC50 of the aqueous extract and rutin which equal to 0.18 and 0.017 mg/ml respectively. According to these results we confirm some information obtained during the ethnobotanical survey and thatJuglans regiaL. is endowed with an antimicrobial and antioxidant power.

2017 ◽  
Vol 6 (5) ◽  
Author(s):  
Zahra Abbasi ◽  
Sholeh Feizi ◽  
Elham Taghipour ◽  
Parinaz Ghadam

AbstractSilver nanoparticles (AgNPs) have widespread applications. Recently, the synthesis of NPs using plant extract has attracted much attention. In this study, with an easy and rapid process at room temperature, AgNPs were produced by the aqueous extract of dried


2021 ◽  
Vol 3 (4) ◽  
pp. 89-95
Author(s):  
Jeff B. Iteku ◽  
Ornella V. Makaya ◽  
Samy Ngunde-te-Ngunde ◽  
Clément L. Inkoto ◽  
Emmanuel M. Lengbiye ◽  
...  

The main objective of our study was to determine the cheminical composition and to assess the antioxidant, antibacterial and antihelminthic activities of the leaves extracts of S. latifolius. The antibacterial activity of S. latifolius leaves extracts was assessed using micro-dilution method. In end, the antioxidant capacity of different plant extracts has been evaluated by determining their ability to reduce iron (III) to iron (II) and DPPH radical. The results obtained revealed that the dry matter of the powder of the S. latifolius leaves is rich in fibers, crude proteins, lipids and carbohydrates. Its water content was moderate (14.3 %); the leaves of S. latifolius are rich in mineral elements like calcium (Ca), magnesium (Mg), potassium (K) and iron (Fe); the plant has the main groups of secondary metabolites such as total polyphenols, flavonoids, tannins, alkaloids, bound and free quinones, steroids and tri-terpenes, while it is poor in anthocyanins, leucoanthocyans and saponins. The bacterial strains used were less sensitive to S. latifolius leaf extracts (MIC greater than or equal to 500 µg/mL). The extracts of this plant showed significant antioxidant and antihelmintic activity, with a strong correlation between the methods used to evaluate the antioxidant potential of DPPH and FRAP. Due to their interesting functional properties, this plant could be used in the formulation of a nutraceutical with strong antioxidant power to improve the antioxidant status of consumers, but also in the management of helminthiases.


Author(s):  
Krishn Kumar Agrawal ◽  
Nitin Agrawal ◽  
Jyoti Gupta ◽  
Tripanshu Gupta

The aim of the present study was to investigate the in-vitro anti-diabetic activity of aqueous and methanolic extract of dried pericarp (Shell) of Juglans regia L. The preliminary qualitative phytochemical screening was also undertaken to reveal the presence of Phyto-constituents in the extracts. Phytochemical screening of aqueous extract shows the presence of polyphenols, flavanoids, cardiac glycosides, carbohydrates and reducing sugar while methanolic extract show the presence of alkaloids, polyphenols, cardiac glycosides, carbohydrates and reducing sugar. Pharmacological evaluation of Juglans regia was performed by α-amylase inhibition assay method. Percentage inhibition of α amylase at dose of 50mg/mL, 100 mg/mL and 150 mg/mL of aqueous and methanolic extract was found to be 33.33, 66.67, 93.34 and 32.27, 59.64, 90.74 respectively. Among the extracts the aqueous extract showed highest activity as compared to control and methanolic extract.


Genes ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 46 ◽  
Author(s):  
Feng Yan ◽  
Huaizhu Li ◽  
Peng Zhao

Juglans regia L. is an economically important crop cultivated worldwide for its high quality and quantity of wood and nuts. Phenylalanine ammonia-lyase (PAL) is the first enzyme in the phenylpropanoid pathway that plays a critical role in plant growth, development, and adaptation, but there have been few reports of the PAL gene family in common walnut. Here, we report a genome-wide study of J. regia PAL genes and analyze their phylogeny, duplication, microRNA, and transcriptional expression. A total of 12 PAL genes were identified in the common walnut and clustered into two subfamilies based on phylogenetic analysis. These common walnut PALs are distributed on eight different pseudo-chromosomes. Seven of the 12 PALs (JrPAL2-3, JrPAL4-2, JrPAL2-1, JrPAL4-1, JrPAL8, JrPAL9, and JrPAL6) were specific found in J. regia, and JrPAL3, JrPAL5, JrPAL1-2, JrPAL7, and JrPAL2-2 were found to be closely associated with the woody plant Populus trichocarpa. Additionally, the expression patterns of JrPAL3, JrPAL7, JrPAL9, and JrPAL2-1 showed that they had high expression in female and male flowers. The miRNA ath-miR830-5p regulates two genes, JrPAL5 and JrPAL1, such that they have low expression in the male and female flowers of the common walnut. Our research provides useful information for further research into the function of PAL genes in common walnut and Juglans.


2020 ◽  
Vol 29 (1) ◽  
pp. 122-127
Author(s):  
A.V. Sinchuk ◽  
S.V. Buga ◽  
S.V. Baryshnikova

The walnut leaf miner Caloptilia roscipennella (Hübner, 1796) is recorded for the first time in Belarus. The larvae of this species damage leaves of the common walnut (Juglans regia L.). In 2015, C. roscipennella was registered in a number of geographical locations in the Brest and Gomel provinces. In subsequent years, the species was found only in the same places. It is shown that the name C. roseipennella Anikin, Zolotukhin et Kirichenko, 2016, syn. nov., being an unjustified emendation, is a junior synonym of C. roscipennella (Hübner, 1796) in accordance with the International Code of Zoological Nomenclature, Articles 32.5.1 and 33.2.3.


2020 ◽  
Vol 20 (9) ◽  
pp. 1504-1513 ◽  
Author(s):  
Ayoub Amssayef ◽  
Mohamed Eddouks

Aims: The current investigation aimed to assess the antioxidant, antidiabetic and antilipidemic effects of the aqueous extract of aerial part of Cotula cinerea (C. cinerea). Background: Cotula cinerea (Del). which belongs to the Asteraceae family is commonly used traditionally for the treatment of diabetes. Objective: The objective of the study was to study the effect of the aqueous C. cinerea extract on glucose and lipid metabolism in normal and streptozotocin-induced diabetic rats using a single and repeated oral administration. Methods: A preliminary phytochemical screening and the quantification of phenolic and flavonoid contents as well as the antioxidant activity using three methods (DPPH, FRAP and ABTS) were carried out. The effect of a single and repeated (15 days of treatment) oral administration of the aqueous extract of aerial part of Cotula cinerea (AEAPCC) at a dose of 20 mg/kg on glucose and lipid profile was examined in normal and streptozotocin-induced diabetic rats. Additionally, histopathological examination of the pancreas and liver was carried out according to the Hematoxylin-Eosin method. Results: AEAPCC (20 mg/kg) showed a significant blood glucose-lowering activity in both normal and diabetic rats after a single and repeated oral administration during 15 days. The aqueous extract was also able to decrease the plasma triglycerides levels in both normal and diabetic rats after 15 days of oral treatment at a dose of 20 mg/Kg while no effect was observed on plasma cholesterol levels. In addition, the results show that AEAPCC exhibits an in vitro antioxidant activity using different tests. Histopathological analysis of the pancreas and liver of AEAPCC-treated diabetic rats has revealed that AEAPCC had a beneficial effect on the architecture of these organs while no improvement of glucose tolerance was noticed using the glucose tolerance test. Furthermore, the results showed that the extract is rich in several phytochemical compounds and exhibited an important antioxidant activity. The phytochemical screening revealed that AEAPCC contains polyphenolic compounds, flavonoids, tannins, alkaloids, saponins, quinones, sterols, terpenoids, anthroquinones and reducing sugars. Whereas, it is free from glycosides. Conclusion: In conclusion, this study demonstrates that Cotula cinerea possesses a beneficial effect on diabetes. Further investigations are required to study the mechanism of action of the antidiabetic effect of this plant.


2016 ◽  
Vol 5 (12) ◽  
pp. 5138
Author(s):  
Shyamji Shukla* ◽  
Priyanka Soni ◽  
Harish K. Kewat

There is an alarming increase in the problem of resistance towards antibiotics amongst most of the pathogenic bacterial strains in recent years. This has drawn the attention of researchers around the world to search for novel and eco-friendly antibacterial compounds. Several biological sources have been explored in this respect but medicinal plants have taken a centre stage out of all. Plants have been known as a reservoir of number of bioactive compounds specially the antibacterial ones since time immemorial. Therefore, the present investigation was undertaken to analyze the antibacterial potential of the medicinal plant Achyranthes aspera. This study revealed that highest antibacterial activity was observed in the methanolic extract of stem against almost all test Bacteria. It showed maximum activity against E.coli (30 mm), followed by S. aureus (28 mm), Enterococcus sp.(25mm), Salmonella typhi ( 20 mm) and least activity was recorded in same extract against K.pneumoniae (6 mm). Four phytochemicals were screened in various solvent extracts. They are alkaloid, flavonoids, saponins and tannins.


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