scholarly journals GC-MS and FT-IR Profiling of leaves methanol extract from the Pleiospermium alatum (Wall. ex Wt. & Arn) Swingle Rutaceae family

2016 ◽  
Vol 5 (5) ◽  
pp. 201-204
Author(s):  
K. Saravanakumar ◽  
◽  
Adaikala Raj ◽  
D. Umaiyambigai ◽  
◽  
...  
Keyword(s):  
Methanol Extract ◽  
Chemical Compounds ◽  
Hexadecenoic Acid ◽  
Bioactive Constituents ◽  
Ms Analysis ◽  
Ft Ir ◽  
Ir Analysis ◽  
Major Chemical ◽  
Potential Use ◽  
Protein Enzyme

The present study was aimed to analysis of bioactive constituents of leaves from the Pleiospermium alatum (Rutaceae). The methanol extract of the leaves were subjected to Fourier transform infrared spectroscopy (FTIR) and Gas chromatography- mass spectroscopic (GC-MS) analysis. GC-MS analysis of plant extract was performed using a Perkin-Elmer GC Clarus 500 system and Interpretation on mass spectrum GC-MS was conducted using the database of National Institute Standard and Technology (NIST) and IR spectrum was recorded in spectrophotometer (Thermo Scientific NICOLET-iS5). FT-IR analysis of peak values with various functional compounds such as amizone, alcohol, phenol, alkanes, protein, enzyme, alkanes, isopropyl. GC-MS analysis of compounds with totally, thirty compounds major chemical compounds were identified, such as 5- Thio-D-glucose, 5-Allylsulfanyl-1-(4-methoxy-phenyl)-1H-tetrazole, E)-10-Heptadecen-8-ynoicacid methyl ester and Z-11-Hexadecenoic acid. The present results concluded that the phytochemicals was observed in methanol extract which revealed that the P. alatum is potential use in different fields namely medical and pharmaceuticals and highly valuable in medicinal usage for the treatment of various human aliments.

scholarly journals Phytochemical screening and in-vitro antibacterial and DPPH free radical scavenging activities of methanol extract of root of Combretum album Pers.

2021 ◽  
Vol 8 (4) ◽  
Author(s):  
Sunanda Burman ◽  
Goutam Chandra
Keyword(s):  
Functional Groups ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Radical Scavenging ◽  
Chloroform Fraction ◽  
Active Ingredients ◽  
Ic50 Value ◽  
Ft Ir ◽  
Ir Analysis

The objective of the study was to investigate in-vitro antibacterial and antioxidant activities of methanolic extract of Combretum album Pers. root. In brief antibacterial efficacies of methanol extract and its petroleum ether fraction (MePET), chloroform fraction (MeCH), ethyl acetate fraction (MeEA) and water fraction (MeAQ) were determined by agar well diffusion assay along with Minimum Inhibitory Concentrations (MICs). In-vitro antioxidant efficacies were evaluated by DPPH radical scavenging method. Preliminary phytochemical assay, Fourier Transform Infrared Spectroscopy and Gas Chromatography-Mass Spectroscopy analyses were employed to detect the plausible active ingredients. Methanol extract showed broad spectrum antibacterial activity having highest inhibition zone against Staphylococcus aureus MTCC 2940 (21.67±0.58 mm) and MICs ranged from100 µg/ml to 250 µg/ml. The scavenging activity of methanol extract of root of C. album was concentration dependant and IC50 value was 136.08 µg/ml. The lowest MIC (5 µg/ml) was noted with MeCH against B. subtilis (MTCC 441). MeCH showed highest antioxidant activity with an IC50 value of 12.98 ?g/mL and MePET, MeEA, MeAQ and Ascorbic acid presented antioxidant potential with IC50 values of 16.10, 15.07, 17.44 and 13.40??g/mL respectively. Preliminary phytochemical tests and FT-IR analysis revealed presence of various phytochemicals and functional groups like hydroxyl, carboxylic acids, amidines, amines, aromatics and esters. Three compounds were elucidated from bioactive TLC fraction of MeCH in GC-MS analysis supported by presence of various functional groups in MeCH as detected in FT-IR analysis. It is concluded that methanolic extract of C. album root is a potential antibacterial and antioxidant agent where presence of N-[5-[4-pyridinyl)-1H-1,2,4-triazol-3-yl]-benzamide (compound 1), pyruvic acid (compound 2) and methyloacetone (compound 3) are responsible as possible active ingredients.

scholarly journals PHYTOCHEMICAL PROFILING, FREE-RADICAL SCAVENGING AND ANTI-INFLAMMATORY ACTIVITIES OF MELIOSMA SIMPLICIFOLIA (L.)

2020 ◽  
pp. 62-68
Author(s):  
S. PAVITHR ◽  
T. SEKAR
Keyword(s):  
Free Radical ◽  
Methanol Extract ◽  
Inflammatory Activity ◽  
Scavenging Activity ◽  
Paw Edema ◽  
Stem Extract ◽  
Ft Ir ◽  
Ir Analysis ◽  
Anti Inflammatory ◽  
Dpph Scavenging

Objective: In the current research, to determine the stem extract of Meliosma simplicifolia (L.) for total phenol, tannin, total flavonoid, antioxidant activity, anti-inflammatory and identify the phytoconstituents utilizing GC-MS and FT-IR. Methods: The ability of the plant extract to act as hydrogen/electrons donor or scavenger of radicals was determined by in vitro antioxidant assays using 2,2-diphenyl-2-picryl-hydrazyl free radical (DPPH) scavenging, reducing power assay, superoxide radical (O2•) scavenging activity. The anti-inflammatory activity was evaluated using carrageenan and formalin-induced paw edema models using Wistar albino rats. The GC-MS and FT-IR analysis of the methanolic stem extract of M. simplicifolia was revealed the presence of phytochemicals. Results: Quantitative studies of estimated phenol, flavonoid and tannin, as for the methanol extract of stem showed the highest content of phenolic compounds (39.83±3.62GAE mg/100). Antioxidant activities were concluded the estimation M. simplicifolia stem for as followed the studies. In stem the methanol extract showed the highest DPPH scavenging activity (124.3µg/ml). The anti-inflammatory activity has shown in high doses of methanolic extract 250 mg/kg of significant value (p<0.05) inhibition of paw edema, on 6th hour, respectively. The FT-IR analysis has confirmed their characteristic peak values and functional groups. Conclusion: M. simplicifolia has an effective of anti-inflammatory activity and constitutes a potential source for the development of new treatments.

Phytochemical and antimicrobial analyses of Plectranthus amboinicus leaf extracts

2021 ◽  
pp. 6379-6384
Author(s):  
M.S. Sindhu ◽  
Poonkothai M.
Keyword(s):  
Antimicrobial Activity ◽  
Petroleum Ether ◽  
Functional Groups ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Leaf Extracts ◽  
Fungal Isolates ◽  
Ft Ir ◽  
Ir Analysis ◽  
Plectranthus Amboinicus

The present study is to evaluate the phytochemical constituents and antimicrobial activity of Plectranthus amboinicus leaf extract using petroleum ether, methanol and water as solvents. The antimicrobial activity of P. amboinicus leaf extracts were examined against the selected bacterial and fungal isolates namely Escherichia coli, Staphylococcus aureus and Aspergillus niger, Candida albicans using agar well diffusion method. FT –IR analysis was performed to identify the presence of functional groups in the methanol extract. The results of the study revealed the presence of distinctive active secondary metabolites in the tested leaf extract. The results revealed that the methanol extract exhibited maximum antimicrobial activity against the tested bacterial and fungal isolates when compared with aqueous and petroleum ether extracts. The FT-IR analysis reveals the presence of functional groups such as hydroxyl, amines, alkynes, ketones and carboxylic acid in the methanolic extract of P. amboinicus. Thus, P. amboinicus leaves are effective against the selected microbes and recommends that the plant derived phytochemicals are comparatively safer than synthetic alternative, thereby contributing insightful remedial benefits for the treatment of diseases.

scholarly journals PRELIMINARY PHYTOCHEMICAL AND FT-IR ANALYSIS OF METHANOLIC LEAVES EXTRACTS OF HEMIDESMUS INDICUS AND TYLOPHORA INDICA

2021 ◽  
Vol 21 (no 1) ◽  
Author(s):  
M. MAHESHWARI ◽  
P. VIJAYARENGAN
Keyword(s):  
Spectral Analysis ◽  
Bioactive Compounds ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Phytochemical Analysis ◽  
Tylophora Indica ◽  
Traditional Medicines ◽  
Hemidesmus Indicus ◽  
Ft Ir ◽  
Ir Analysis

Medicinal plants are of great importance for the health of individuals and communities. Hemidesmus indicus and Tylophora indica are used as traditional medicines. These plants contain a lot of bioactive compounds. The present investigation is carried out in Hemidesmus indicus and Tylophora indica leaves. In the present study, the methanol extract of H. indicus and T. indica were subjected for preliminary phytochemical analysis through FT-IR spectral analysis and the bioactive compounds were identified. The Preliminary Phytochemical analysis of methanolic leaves extract of H.indicus and T.indica revealed the presence of secondary metabolites. FT-IR analysis of methanolic leaf extract of Hemidesmus indicus and Tylophora indica revealed 16 and 13 major compounds respectively. The H.indicus has higher bioactive compounds compared to T.indica.

Potential Use of Galium verum Essential Oil for Antibacterial Properties in Gelatin Based Hydrogels Prepared by Microwave Irradiation Technique

2018 ◽  
Vol 69 (3) ◽  
pp. 575-580 ◽  
Author(s):  
Timea Gherman ◽  
Violeta Popescu ◽  
Rahela Carpa ◽  
Maria Rapa ◽  
Georgiana Luminita Gavril ◽  
...  
Keyword(s):  
Essential Oil ◽  
Chemical Interaction ◽  
Synergistic Effects ◽  
Antibacterial Properties ◽  
Diffusion Method ◽  
Hydrogel Matrix ◽  
Ft Ir ◽  
Ir Analysis ◽  
Polymerization Method ◽  
Potential Use

Galium verum essential oil (EO) loaded gelatin hydrogel was prepared by microwave-assisted polymerization method. FT-IR analysis indicated no chemical interaction between the hydrogel matrix and EO. Good swelling behavior, increased thermo- and mechanical- properties was attributed to the synergistic effects of the secondary structure of gelatin after gelation and intermolecular hydrogen-bonding interactions between the hydrogel matrix and EO ingredient�s. Antibacterial activity was investigated by the agar diffusion method.

scholarly journals Caracterização dos Compostos Químicos dos Óleos Extraídos da Casca, Arilo e Semente dos Frutos de Hymenaea stigonocarpa Mart. ex Hayne (Jatobá-do-Cerrado)

2019 ◽  
Vol 23 (3) ◽  
pp. 171
Author(s):  
Antonio Carlos Pereira de Menezes Filho ◽  
Karla Da Silva Malaquias ◽  
Carlos Frederico de Souza Castro
Keyword(s):  
Gas Chromatography ◽  
Seed Oil ◽  
Chemical Compounds ◽  
Infrared Region ◽  
Fruit Species ◽  
Chemical Constitution ◽  
Ft Ir ◽  
Antioxidant Agent ◽  
Pharmaceutical Industries ◽  
Ir Analysis

O domínio Cerrado apresenta uma flora magnífica, composta por espécies frutíferas de grande porte, como o jatobá-do-cerrado (Hymenaea stigonocarpa), que produz anualmente volumosas quantidades de frutos, que servem de alimento para a fauna e moradores locais. O trabalho tem por objetivo avaliar, por técnicas cromatográficas por CG-MS e espectrométricas na região do infravermelho, os óleos extraídos da casca, arilo e sementes desta leguminosa. Avaliando também o rendimento de óleo extraído pelo sistema de soxlhet. Foram obtidos rendimentos lipídicos de 3,94, 1,83 e 3,47% respectivamente para casca, arilo e semente. Os compostos químicos avaliados por cromatografia gasosa (CG-MS) apresentaram compostos como: 4,6-di (1,1 - dimetiletil)-2-metil fenol, um agente antioxidante natural encontrado em vegetais,  que neste estudo foi encontrado nos óleos da casca e arilo com teores de 18,90 e 11,90% respectivamente. Teores importantes de ácido isoesteárico de 45,80% no óleo do arilo, e para o óleo da semente, porcentagens expressivas de palmitato de metila 52,90; ácidos heneicosanóico de 38,40% e de pentacosanóico igual a 46,20%. A análise de FT-IR apresentou grupamentos funcionais característicos de ácidos graxos como observados normalmente em outros óleos vegetais. O trabalho fornece dados importantes sobre as características químicas dos óleos extraídos do fruto de H. stigonocarpa, aumentando assim o conhecimento sobre esta espécie da família Leguminosae, que está presente no domínio Cerrado, bem como compor conhecimento científico no uso racional destes óleos nas indústrias alimentícia e farmacêutica. Palavras-chave: Espectroscopia no Infravermelho. Cromatografia Gasosa. Constituição Química Abstract Cerrado domain presents a magnificent flora, composed of large fruit species, such as  jatobá-do-cerrado (Hymenaea stigonocarpa), which annually produces large quantities of fruits that serve as food for the fauna and local inhabitants. The objective of this work is to evaluate the oils extracted from the bark, aril and seeds of this legume by GC-MS and spectrometric techniques in the infrared region. Also, to evaluate  the oil yield extracted by the soxlhet system. Lipid yields of 3.94, 1.83 and 3.47% respectively were obtained for bark, aril and seed. The chemical compounds evaluated by gas chromatography (GC-MS) presented compounds as 4,6-di (1,1-dimethylethyl) -2-methyl phenol, a natural antioxidant agent found in vegetables, where in this study it was found in bark oils and aryl with contents of 18.90 and 11.90% respectively. Important isostearic acid contents of 45.80% in aryl oil, and for seed oil, significant percentages of methyl palmitate 52.90; heneicosanoic acids of 38.40% and pentacosanoic acids equals to 46.20%. The FT-IR analysis showed functional groupings characteristic of fatty acids as normally observed in other vegetable oils. This work provides important information on the chemical characteristics of the oils extracted from the H. stigonocarpa fruit, thus increasing the knowledge about this species of the Leguminosae family that is present in the Cerrado domain, as well as to compose scientific knowledge on the rational use of these oils in the food and pharmaceutical industries. Keywords: Infrared Spectroscopy. Gas Chromatography. Chemical Constitution.

scholarly journals Chemical Constituents of Buah Makasar [Brucea Javanica (L) Merr] Leaves and Seed Extract

2019 ◽  
Vol 2 (2) ◽  
pp. 99
Author(s):  
Handa Muliasari ◽  
Candra D Hamdin ◽  
Agus Dwi Ananto ◽  
Muhsinul Ihsan
Keyword(s):  
Methanol Extract ◽  
Chemical Constituents ◽  
Chemical Compounds ◽  
Seed Extract ◽  
Chemical Compositions ◽  
Phytochemical Screening ◽  
Brucea Javanica ◽  
Ms Analysis ◽  
Local Plants ◽  
Lombok Island

Buah Makasar [Brucea javanica (L) Merr] is one of the local plants in Lombok Island that is traditionally used for diabetic medication. The purpose of this research was to identify the chemical constituents containing in methanol leaves and seed extract of Buah Makasar. The methods used were phytochemical screening for some groups of chemical compounds and GC-MS analysis. The result of phytochemical screening showed that methanol extract of B. javanica seed contains alkaloids, terpenoids, and tannin with a high intensity; while the leaves extract contains alkaloids, flavonoids, steroids, tannin, and saponin. The differences of chemical compositions in the leaves and seed extract may give them have different bioactivities. GC-MS analysis showed some fatty acids containing in the seed extract such as stearic acid and palmitic acid.

scholarly journals Screening and Evaluation of Bioactive Components of Methanol Root Extract of Anthocleista nobilis G. Don. by GCMS Analysis

2020 ◽  
Author(s):  
Adekunle ORIMISAN Ojatula
Keyword(s):  
Methanol Extract ◽  
Biological Activities ◽  
Biologically Active ◽  
Root Extract ◽  
Bioactive Constituents ◽  
Active Metabolites ◽  
Ms Analysis ◽  
Medicinal Value ◽  
Study Results ◽  
Phytochemical Compounds

Abstract Background: Medicinal plants have great importance in African medicine and are also used as precursors in drug discovery. The medicinal value of plants lies in their bioactive constituents which usually allow them to fight against several diseases. Plant-based natural constituents can be derived from any part of the plant like roots, bark, leaves, flowers, fruits, seeds. The present study was designed to determine the bioactive/biochemical compounds present in the methanol extract of Anthocleista nobilis root.Materials and Methods: GC -MS analysis of the methanol extract of A. nobilis root was performed using a Perkin Elmer GC Clarus 500 system comprising an Agilent technologies 5975 MSD model detector and a gas chromatograph interfaced to a mass spectrometer with the aid of the Turbo mass 5.0 software.Results: The study results of the GC-MS analysis provided different phytochemical compounds possessing several biological activities such as antimicrobial, antifungal, anticancer, and anti-inflammatory activities. This study therefore, showed that root of Anthocleista nobilis is a source of biologically active metabolites. Furthermore, root extract revealed the presence of diverse chemical constituents.Conclusion: The results of the present study suggest a recommendation of A. nobilis root as a plant of phytopharmaceutical importance.

Phytochemical Profile and Antifungal Activity of Leaves Methanol Extract from the Psydrax dicoccos (Gaertn) Teys. & Binn. Rubiaceae Family

2017 ◽  
Vol 7 ◽  
pp. 53-61 ◽  
Author(s):  
D. Umaiyambigai ◽  
K. Saravanakumar ◽  
G. Adaikala Raj
Keyword(s):  
Antifungal Activity ◽  
Cinnamic Acid ◽  
Methanol Extract ◽  
Microsporum Gypseum ◽  
Hexadecanoic Acid ◽  
Ms Analysis ◽  
Zones Of Inhibition ◽  
Ft Ir ◽  
The Mean ◽  
Phytochemical Profile

The present study was aimed to antifungal activity and phytoconstituents of leaves from thePsydrax dicoccosGaertn.The antifungal activity of methanol extract from theP. dicoccosagainstCandida albicans,C. krusei,C. guilliermondii,C. parapsilosis,C. tropicalis, C.glabrata, four dermatophytesviz.,Trichophyton rubrum,T. mentagrophytes,Microsporum gypseumandEpidermophyton flocossum.The methanol extract of the leaves were subjected to Fourier transform infrared spectroscopy (FT-IR) and GasChromatography-Mass Spectroscopic (GC-MS) analysis. The mean zones of inhibition produced by the tested extract in disc diffusion assays against fungal strains were ranged from 7.3 to 15.5 mm. The MIC values were between 125 and 500 µg/ml while, the MFC values were between 250 and 1000 µg/ml. The highest mean zones of inhibition (15.5±0.6 mm) was observed with methanol extract ofP. dicoccosagainstC. albicans. The GC-MS analysis ofP. dicoccosleaves showed the presence of cinnamic acid, 2H-1-Benzopyran-2-one,5,7-dimethoxy,(Z)6,(Z)9-Pentadecadien-1-ol, Benzofuran and n-Hexadecanoic acid as major compounds. Finally it can be concluded that the antifungal activity may be present in cinnamic acid, 2H-1-Benzopyran-2-one, 5, 7-, (Z) 6,(Z)9-Pentadecadien-1-ol, n-Hexadecanoic acid from the methanol extract from theP. dicoccosis highly valuable in medicinal usage and have fewer side effects.

scholarly journals Secondary Metabolites of the Cuticular Abdominal Glands of Variegated Grasshopper (Zonocerus variegatus L.)

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
O. U. Igwe ◽  
D. E. Udofia
Keyword(s):  
Methyl Ester ◽  
Octadecenoic Acid ◽  
Acid Methyl Ester ◽  
Chemical Compounds ◽  
Gas Chromatography Mass Spectrometry ◽  
Zonocerus Variegatus ◽  
Acid Methyl ◽  
Tridecanoic Acid ◽  
Ft Ir ◽  
Ir Analysis

Chemical compounds were extracted with petroleum ether from the cuticular abdominal glands of grasshopper (Zonocerus variegatus L.) and eleven compounds were characterised using Gas Chromatography/Mass Spectrometry (GC/MS) technique in combination with Fourier Transform-Infrared Spectroscopy (FT-IR). The compounds analysed were 2,7-dimethyloctane (3.21%), decane (5.33%), undecane (3.81%), tridecanoic acid methyl ester (4.76%), hexadecanoic acid (9.37%), 11-octadecenoic acid methyl ester (23.18%), pentadecanoic acid, 14-methyl-methyl ester (4.43%), (Z)-13-docosenoic acid (10.71%), dodecyl pentafluoropropionate (9.52%), 2-dodecyl-1,3-propanediol (6.38%), and 1,12-tridecadiene (19.30%). FT-IR analysis of the extract showed peaks at 1270.17 (C–O and C–F), 1641.48 (C=C), 2937.68 (C–H), and 3430.51 (O–H) cm−1 indicating the presence of ether, alkene, alkane, alcohol, carboxylic acid, and fluoric compounds. These compounds consisted of 32.37% ester, 31.65% hydrocarbons, 20.08% fatty acid, 9.52% halogenated ester, and 6.38% alcohol. The highest component was 11-octadecenoic acid methyl ester followed by 1,12-tridecadiene. Since behavioural bioassays were not carried out, the consideration of these compounds to be pheromone semiochemicals remains a hypothesis.

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