The relevance of antral follicle count (AFC) as a marker of ovarian reserve in normal and infertile women in a tertiary care centre in Central India

Background: The objective of the present study was to establish the role of AFC as a marker of ovarian reserve in fertility-proven and in sub-fertile Indian women, and to establish the baseline cut-off AFC values for Indian women.Methods: This is an Observational Case-Control type of study. Test subjects (n=30, age range 20-35 years) were selected randomly from all the women coming to our Out-Patient Department with Primary Infertility. Healthy female volunteers (n=30, age range 20-35years) were recruited from the local population. Statistics: Student t test was applied to compare the mean Antral Follicle count between the case and control group. Pearson correlation test was applied to assess the correlation between age and AFC for case and control group.Results: The results of the study show that there is a significant difference between the number of antral follicles between fertile and infertile women (p < 0.001). The baseline cut-off for successful pregnancy was established as an AFC of 12. There exists negative correlation (r = -0.249) between age and AFC case group indicating as the age increases AFC decreases.Conclusions: The findings from this study help in strengthening the idea that AFC serves as a marker for ovarian reserve. Our observation indicates that the number of antral follicles is lower in the subfertile patients as compared to the fertile group (in all age groups), in view of the significantly lower median AFC in women of the former group (P < 0.001).

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Should fertile women quit drinking alcohol to produce better quality oocytes?

Zygote ◽

10.1017/s0967199420000696 ◽

2020 ◽

pp. 1-3

Author(s):

Burcu Ozbakir ◽

Pinar Tulay

Keyword(s):

Alcohol Consumption ◽

Young Women ◽

Antral Follicle ◽

Antral Follicle Count ◽

Control Group ◽

Social Drinkers ◽

Antral Follicles ◽

Healthy Women ◽

Significant Difference

Summary Alcohol consumption has long been shown to affect both fetal health and pregnancy. In this study, antral follicle count, maturation level of oocytes including morphological assessment and number of metaphase I (MI), metaphase II (MII) and germinal vesicle (GV) stage oocytes obtained from young women (age < 30 years old) with or without alcohol consumption were investigated. In total, 20 healthy women who were social drinkers and 36 healthy women who do not consume alcohol were involved in this study. Women in both study and control groups were undergoing controlled ovarian stimulation. The antral follicle count and the number and quality of the oocytes retrieved were evaluated and recorded. In total, 635 antral follicles, 1098 follicles and 1014 oocytes with 820 MII, 72 MI and 78 GV stage oocytes were collected from the social drinkers. In the control group, 628 antral follicles, 1136 follicles and 1085 oocytes with 838 MII, 93 MI and 102 GV stage oocytes were evaluated. The results of this study showed that the antral follicle count was very similar in both groups. The number of oocytes and MII stage oocytes was slightly higher in the control group, although it was not a significant difference. This study showed that although the consumption of alcohol may have adverse effects post-implantation, it may not have a solid effect during oogenesis in young women. The results of this study are especially important in clinical settings as some women who are social drinkers undergo in vitro fertilization treatments.

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Is There a Need to Alter the Timing of Anti-Müllerian Hormone Measurement During the Menstrual Cycle?

Geburtshilfe und Frauenheilkunde ◽

10.1055/a-0840-3817 ◽

2019 ◽

Vol 79 (07) ◽

pp. 731-737 ◽

Cited By ~ 1

Author(s):

Umit Gorkem ◽

Cihan Togrul

Keyword(s):

Menstrual Cycle ◽

Ovarian Reserve ◽

Luteal Phase ◽

Antral Follicle ◽

Antral Follicle Count ◽

Infertile Women ◽

Significant Difference ◽

Response Groups ◽

N 93 ◽

Response Group

Abstract Introduction There are numerous conflicting studies which have addressed the question whether the measurement of anti-Müllerian hormone (AMH) concentrations should be done at a certain time during the menstrual cycle. We aimed to investigate AMH fluctuations during the follicular and luteal phases of the menstrual cycle and to determine whether AMH variations, if present, might influence the clinical utility of ovarian reserve markers. Materials and Methods A total of 257 infertile women eligible for inclusion were categorized into three groups based on their total antral follicle count: 1. hypo-response group (< 7 follicles, n = 66), 2. normo-response group (7 – 19 follicles, n = 98), and 3. hyper-response group (> 19 follicles, n = 93). Results Mean follicular AMH levels were elevated compared to levels in the luteal phase in all response groups (p < 0.001). There were significant and strong positive correlations between follicular and luteal AMH levels in all response groups (Spearmanʼs r = 0.822, r = 0.836, and r = 0.899, respectively; p < 0.001 for all groups). Fisherʼs Z-test comparisons of these correlations in all response groups demonstrated that there was no statistically significant difference (Z = 0.277, Z = − 1.001, and Z = − 1.425, respectively; p < 0.001). Conclusion We found that serum AMH levels in the follicular phase were higher than those in the luteal phase in all three response groups. In current practice, fluctuations in serum AMH concentrations are not large enough to alter the timing of AMH measurements during the menstrual cycle. The issue is important for the assessment of ovarian reserve in infertile women with AMH levels near to the cut-off value.

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The effectiveness of collaborative infertility counseling (CIC) on pregnancy outcome in infertile women undergoing in vitro fertilization: A randomized controlled trial

10.21203/rs.2.14093/v1 ◽

2019 ◽

Author(s):

Mahboobeh Rasoulzadeh Bidgoli ◽

robab latifnejad roudsari ◽

ali montazeri

Keyword(s):

In Vitro Fertilization ◽

Pregnancy Rate ◽

Treatment Success ◽

Intervention Group ◽

Control Group ◽

Vitro Fertilization ◽

Infertile Women ◽

Significant Difference ◽

And Control

Abstract Background: Infertility is an emotional tension which influences the whole aspects of relationships in infertile couples. A main objective of infertility treatments is elevation of pregnancy rate. The present study aimed to examine the effect of collaborative counseling on pregnancy rate in infertile women, undergoing in vitro fertilization in Mashhad, Iran. Methods: In this clinical trial, 60 women with primary infertility were selected from an infertility research center and were randomly allocated into intervention (n=29) and control (n=31) groups. The intervention group received individual counseling, based on the collaborative reproductive healthcare model with collaboration of a midwife, a gynecologist and a clinical psychologist in five sessions during a two-month period. The control group received routine care. Positive pregnancy test was considered as a criterion of treatment success at the end of the study. Data were analyzed using statistical tests including independent samples t-test. Results: There was no significant difference in pregnancy rate between intervention and control groups (P = 0.298). Also, there were no significant differences in follicle and embryo numbers between two groups. However, a significant difference was observed between two groups in terms of oocyte numbers where the intervention group had more oocyte (P = 0.014). Conclusion: Overall the findings indicated that the collaborative infertility counseling did not improve treatment success in infertile women undergoing in vitro fertilization

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Value of serum and follicular fluid Sirt1,Sirt2 protein levels in predicting the outcome of assisted reproduction

10.21203/rs.2.23749/v1 ◽

2020 ◽

Author(s):

Lingnv Yao ◽

Wenqin Lin ◽

Nan Jiang ◽

Chuyan Li ◽

Haifeng Cao ◽

...

Keyword(s):

Assisted Reproduction ◽

Follicular Fluid ◽

Ovarian Reserve ◽

Antral Follicle ◽

Antral Follicle Count ◽

Advanced Age ◽

Control Group ◽

Age Group ◽

Protein Levels ◽

Study Population

Abstract Background: To explore whether serum and follicular fluid Sirt1, Sirt2 can reflect ovarian reserve and predict the outcome of assisted reproduction. Methods: The study population comprised 125 patients , 39 in OPOI（occult form of premature ovarian insufficiency）group, 49 in advanced age group , and 37 in control group. The levels of serum Sirt1, Sirt2 were measured on the 2nd to 5th day of menstruation (bSirt1,bSirt2) and HCG day. Follicular fluid Sirt1 (FFSirt1) and Sirt2 (FFSirt2), were determined on OPU (oocyte pick up) day. Results: The level of FFSirt2 in the advanced age group was significantly lower than those in other two groups. FFSirt 2 and Sirt 2 (HCG day ) were negatively correlated with age (r=-0.35, r=-0.19), but there were no value of them for assessing DOR (diminished ovarian reserve). The level of bSirt2 in (cumulative) pregnant group was significantly higher (r=0.24, P=0.00). Conclusions: This was the first study to show that FFSirt2 and Sirt2 (HCG day) might be negatively correlated with age and antral follicle count (AFC). bSirt2 could predict cumulative pregnancy outcome together with anti-Mullerian hormone (AMH), AFC and age.

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Correlation Between Follicle Stimulating Hormone, Anti-Müllerian Hormone and Antral Follicle Count with Different Age Groups in Infertile Women

Chattagram Maa-O-Shishu Hospital Medical College Journal ◽

10.3329/cmoshmcj.v20i1.53582 ◽

2021 ◽

Vol 20 (1) ◽

pp. 22-27

Author(s):

Juthi Bhowmik ◽

Parveen Fatima ◽

Jesmine Banu ◽

Farzana Deeba ◽

Sheuli Chowdhury ◽

...

Keyword(s):

Negative Correlation ◽

Ovarian Reserve ◽

Follicle Stimulating Hormone ◽

Age Groups ◽

Antral Follicle ◽

Antral Follicle Count ◽

Age Group ◽

Infertile Women ◽

Group A ◽

Study Population

Background: Reduced ovarian reserve predicts poor ovarian response and poor suc-- cess rates in infertile women who undergo Assisted Reproductive Technology (ART). Ovarian reserve decreases with age but the rate of decline varies from one woman to another. Follicle Stimulating Hormone (FSH) Anti-Müllerian Hormone (AMH) and antral follicle count (AFC) represent the three most frequently utilized laboratory tests in determining Ovarian Reserve (OR). To determine correlation between FSH, AMH and AFC in infertile female. Materials and methods: It was an observational (Cross sectional) study. This study was done in the Department of Reproductive Endocrinology and Infertility, Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, between July 2018 to June 2019. The study population consisted of all the diagnosed female infertility patients of reproductive age. The women attending the study center during study period having primary or secondary infertility was considered as study population. They were divided in 4 age groups 21-25, 26-30, 31-35 yrs and 36-40 yrs years. Data was collected using a structured questionnaire following physical & lab examination. For D2 FSH level fasting blood was collected on D2/3 of menstrual cycle, serum FSH level was measured by ADVIA Centraur(R) XP immunoassay system. For S. AMH level blood sample was collected on 2nd day of cycle and measured by BECKMAN COULTER machine using Chemiluminescent Immunoassay method. For AFC count TVS was done on D2-5 of cycle using KONTRON medical USG machine. Collected data were classified, edited, coded and entered into the computer for statistical analysis by using SPSS version 23. Results: Out of 74 patients the mean age was found 32.6±5.5 years. Serum FSH, AMH and AFC were significantly associated with different age group. A negative correlation was found between serum FSH and serum AMH in all age group. But strong correlation found in age group 31-35 yrs and in 36-40 years age group. A negative correlation was found between serum FSH and total AFC in age group 26- 30 years, 31-35 years and 36-40 years respectively. A positive correlation was found between serum AMH and total AFC in all age group but most strong in age group 31-35 years. In multivariate logistic regression analysis serum AMH (<1.0 ng/ml) and total AFC (<5 number) were found to be significantly associated with age group >35 years patients. Conclusion: In all age grqoup, FSH, AMH and AFC correlates but it is more pronounced in advanced age that means >35 years age group. Chatt Maa Shi Hosp Med Coll J; Vol.20 (1); January 2021; Page 22-27

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Ovarian reserve in patients who have undergone endometriosis surgery

Vojnosanitetski pregled ◽

10.2298/vsp160408363d ◽

2018 ◽

Vol 75 (7) ◽

pp. 644-650

Author(s):

Olivera Dzatic-Smiljkovic ◽

Mladenko Vasiljevic ◽

Ivana Rudic ◽

Jelena Vugdelic ◽

Aleksandar Ristic ◽

...

Keyword(s):

Ovarian Reserve ◽

Ovarian Function ◽

Uterine Cavity ◽

Antral Follicle ◽

Antral Follicle Count ◽

Inhibin B ◽

Ca 125 ◽

Control Group ◽

Study Group ◽

Ovarian Volume

Background/Aim. Endometriosis is a gynaecological disorder characterized by the presence of endometrial tissue outside the uterine cavity. The aim of this paper was to determine the effect of laparoscopic cystotomy and cystectomy on ovarian function, as well as to compare these two methods in terms of qualitative and quantitative damage to the ovaries, achieved pregnancies and recurrence. Methods. The prospective study, conducted in ?Narodni Front? Obstetrics and Gynaecology Clinic in Belgrade at the Endoscopic Infertility Treatment Ward, included a total of 150 patients. The study group was represented by 100 patients who underwent a surgical treatment of endometrial ovarian cysts. The patients in the study group were divided into 2 subgroups: Subgroup I consisted of 50 patients who underwent a laparoscopic cystotomy (incision of the cyst and thermal coagulation) and subgroup II which included 50 women who underwent a laparoscopic cystectomy. The control group consised of patients who underwent a surgery due to tubal factor infertility. The following parameters of the ovarian function were tested: the ovarian volume, the antral follicle count, the presence of the preovulatory follicle on the operated ovary, the serum levels of anti- M?llerian hormone (AMH), follicle-stimulating hormone (FSH), ovarian tumor marker (Ca 125), inhibin B, as well as the rate of achieved pregnancies one year after the surgery. Results. The ovarian volume and the antral follicle count as well as the FSH values were significantly higher in the control group in comparison with the patients in the study group. There were no significant differences in the ovarian volume, the antral follicle count, the AMH values and inhibin B values in the study group between the patients with cystectomy and those with the incision and coagulation of the cyst. Conclusion. Both surgical techniques diminished the ovarian reserve: cystectomy was more aggressive method in terms of the damage inflicted on the ovarian tissue, and incision with coagulation carried a higher risk of recurrence.

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Pediatric Functional Constipation and Whey Protein: A Randomized Clinical Trial

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2021/v33i1531286 ◽

2021 ◽

pp. 45-55

Author(s):

Fateme Motavasselian ◽

Majid Emtiazy ◽

Mehrdad Shakiba ◽

Majid Aflatoonian

Keyword(s):

Whey Protein ◽

Gastrointestinal Disease ◽

Protein A ◽

Intervention Group ◽

Functional Constipation ◽

Control Group ◽

P Value ◽

Significant Difference ◽

Age Range ◽

And Control

Background: Functional constipation is a chronic gastrointestinal disease in children. Pharmacological treatment of constipation is mostly based on the prescription of laxatives. In addition to this treatment, complementary therapies were also proposed to treat constipation. In this study, the effect of whey protein in the treatment of constipation was investigated. Method and Materials: A total of 56 children with functional constipation within the age range of 1 to 16 years were included in the study. Patients were randomly divided into the intervention (n=28) and control (n=28) groups. The intervention group received 15 g whey protein and the control group received 15 g placebo. Both groups received 0.4 - 0.8 gr/kg PEG powder. The amount of PEG was adjusted every four days based on having soft stools. Demographic information were collected and recorded. Furthermore, fecal consistency, stool frequency, symptoms of stool retention, fecal incontinence, and abdominal pain were recorded prior to the intervention and at the second and fourth weeks of the intervention. Results: All patients had soft daily stools at the end of the intervention. At the end of the fourth week, the mean required amounts of PEG powder were respectively 0.62 and 0.28 g/kg in the control and intervention groups, which showed a significant difference (p value ≤0.001). Conclusion: Based on these findings, whey protein is safe and can improve constipation. However, more studies with larger sample size and longer follow-up period are needed to confirm the results of this study.

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Association Between Cytomegalovirus Infection and Bad Obstetric Outcomes in Women from Kirkuk

International Journal of Public Health Science (IJPHS) ◽

10.11591/ijphs.v3i1.4672 ◽

2014 ◽

Vol 3 (1) ◽

pp. 29

Author(s):

Hala Mohamed Majeed Hassan ◽

Abdulghani Mohamed Alsamarai ◽

Zainab Khalil Aljumaili ◽

Amina Hamed Alobaidi ◽

Ferah Ghali Alsalihi

Keyword(s):

Viral Infections ◽

Obstetric Outcomes ◽

Control Group ◽

Serum Samples ◽

Previous Pregnancy ◽

Obstetric History ◽

Significant Difference ◽

Animal Exposure ◽

Age Range ◽

And Control

The human cytomegalovirus (CMV) is one of the common viral infections worldwide that represent a major causes of congenital infections. To determine the seroprevalence of CMV in women with bad obstetric history and sociodemographic characteristics that may influence the seropositivity, a case control descriptive prospective study was conducted in Kirkuk, Iraq. A 838 women with age range from 14 to 48 were included in the study. Of the total, 547 women were with bad obstetric history(BOH) and 291 women with normal previous pregnancy as control group. All the serum samples collected from the study and control groups were tested for CMV IgM and IgG antibodies by ELISA kits. CMV IgM seroprevalence was higher in women with BOH. CMV IgG seroprevalence was with no significant difference between BOH and control. CMV IgG seroprevalence significantly influenced by age, education, smoking, and family size. However, CMV IgM seroprevalence significantly associated with pregnancy, residence, and animal exposure. Odd ratio confirmed the association between CMV IgG and age, crowding index, residence, smoking, and number of abortion in women with BOH. In addition, current CMV infection significantly associated with residence in women with BOH.

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Effects of per oral cypermethrin exposure on Bcl-2 expression in granulose cells and antral follicle count of Rattus norvegicus ovaries

Majalah Obstetri & Ginekologi ◽

10.20473/mog.v26i32018.123-127 ◽

2019 ◽

Vol 26 (3) ◽

pp. 123 ◽

Cited By ~ 1

Author(s):

Indrayanti Indrayanti ◽

Bambang Rahardjo ◽

Hidayat Sujuti

Keyword(s):

Treatment Group ◽

Group Treatment ◽

Rattus Norvegicus ◽

Antral Follicle ◽

Antral Follicle Count ◽

Exposed Group ◽

Immunohistochemical Method ◽

Control Group ◽

Control Group Design ◽

Antral Follicles

Objectives: To determine the effect of oral cypermethrin exposure on the decrease of Bcl-2 expression in granulosa cells and antral follicle count in Rattus norvegicus ovary.Materials and Methods: This was a true experimental study using post-test control group design. This study used 24 Rattus norvegicus which were divided into 4 groups, the control group, treatment group (P1) exposed to cypermethrin 10 mg/kg BW, treatment group (P2) exposed to cypermethrin 15 mg/kg BW and treatment group (P3) exposed to cypermethrin 20 mg/kg BW. The treatment groups were exposed to cypermethrin for 28 days. A surgery was conducted in proestrus phase and ovarian organs were taken. Bcl-2 expression examination was performed by using immunohistochemical method and the antral follicle count was assessed with the Hematoxillin Eosin (HE)Results: This study showed the decrease of Bcl-2 expression in cypermethrin-exposed group compared to control group. There was a decrease in antral follicles count in cypermethrin-exposed group compared to control group.Conclusion: Cypermethrin can decrease Bcl-2 expression and antral follicles count in Rattus norvegicus ovaries.

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