scholarly journals Effects of per oral cypermethrin exposure on Bcl-2 expression in granulose cells and antral follicle count of Rattus norvegicus ovaries

2019 ◽  
Vol 26 (3) ◽  
pp. 123 ◽  
Author(s):  
Indrayanti Indrayanti ◽  
Bambang Rahardjo ◽  
Hidayat Sujuti
Keyword(s):  
Treatment Group ◽  
Group Treatment ◽  
Rattus Norvegicus ◽  
Antral Follicle ◽  
Antral Follicle Count ◽  
Exposed Group ◽  
Immunohistochemical Method ◽  
Control Group ◽  
Control Group Design ◽  
Antral Follicles

Objectives: To determine the effect of oral cypermethrin exposure on the decrease of Bcl-2 expression in granulosa cells and antral follicle count in Rattus norvegicus ovary.Materials and Methods: This was a true experimental study using post-test control group design. This study used 24 Rattus norvegicus which were divided into 4 groups, the control group, treatment group (P1) exposed to cypermethrin 10 mg/kg BW, treatment group (P2) exposed to cypermethrin 15 mg/kg BW and treatment group (P3) exposed to cypermethrin 20 mg/kg BW. The treatment groups were exposed to cypermethrin for 28 days. A surgery was conducted in proestrus phase and ovarian organs were taken. Bcl-2 expression examination was performed by using immunohistochemical method and the antral follicle count was assessed with the Hematoxillin Eosin (HE)Results: This study showed the decrease of Bcl-2 expression in cypermethrin-exposed group compared to control group. There was a decrease in antral follicles count in cypermethrin-exposed group compared to control group.Conclusion: Cypermethrin can decrease Bcl-2 expression and antral follicles count in Rattus norvegicus ovaries.

Should fertile women quit drinking alcohol to produce better quality oocytes?

Zygote ◽  
2020 ◽  
pp. 1-3
Author(s):  
Burcu Ozbakir ◽  
Pinar Tulay
Keyword(s):  
Alcohol Consumption ◽  
Young Women ◽  
Antral Follicle ◽  
Antral Follicle Count ◽  
Control Group ◽  
Social Drinkers ◽  
Antral Follicles ◽  
Healthy Women ◽  
Significant Difference

Summary Alcohol consumption has long been shown to affect both fetal health and pregnancy. In this study, antral follicle count, maturation level of oocytes including morphological assessment and number of metaphase I (MI), metaphase II (MII) and germinal vesicle (GV) stage oocytes obtained from young women (age < 30 years old) with or without alcohol consumption were investigated. In total, 20 healthy women who were social drinkers and 36 healthy women who do not consume alcohol were involved in this study. Women in both study and control groups were undergoing controlled ovarian stimulation. The antral follicle count and the number and quality of the oocytes retrieved were evaluated and recorded. In total, 635 antral follicles, 1098 follicles and 1014 oocytes with 820 MII, 72 MI and 78 GV stage oocytes were collected from the social drinkers. In the control group, 628 antral follicles, 1136 follicles and 1085 oocytes with 838 MII, 93 MI and 102 GV stage oocytes were evaluated. The results of this study showed that the antral follicle count was very similar in both groups. The number of oocytes and MII stage oocytes was slightly higher in the control group, although it was not a significant difference. This study showed that although the consumption of alcohol may have adverse effects post-implantation, it may not have a solid effect during oogenesis in young women. The results of this study are especially important in clinical settings as some women who are social drinkers undergo in vitro fertilization treatments.

scholarly journals Short Communication: Effects of black soy phytoestrogens (Glycine soja) on elevated levels of estradiol in rat blood (Rattus novergicus) ovariectomy

2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Dwi Yuliawati ◽  
WURI WIDI ASTUTI ◽  
FITRI YUNIARTI
Keyword(s):  
Group Treatment ◽  
Rattus Norvegicus ◽  
Glycine Soja ◽  
Negative Control ◽  
Female Rats ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
P Value ◽  
Control Group Design ◽  
Rat Blood

Abstract. Yuliawati D, Astuti WW, Yuniarti F. 2020. Effects of Black Soy phytoestrogens (Glycine soja) on elevated levels of estradiol in Rat Blood (Rattus norvegicus) ovariectomy. Nusantara Bioscience 12: 55-58. Menopause is the transition from productive to non-productive times due to reduced estrogen and progesterone and has an impact on improving cardiovascular problems. The fulfillment of estrogen needs in menopause can use a type of phytoestrogens from black soybeans. The purpose of research is to prove the administration of black soy extract (Glycine soja) can increase the levels of estradiol in the rat blood (Rattus norvegicus) ovariectomy. Design research uses post-test only control group design. Twenty-five female rats were randomly divided into 5 groups (n = 5 tails per group), negative controls, positive and 3 treatments. Ovariectomy was performed in a group of positive controls and treatment. Thirty-day post ovariectomy rats were given appropriate group treatment (negative control, positive, and treatment with the administration of black soy extract dose of 50, 100, 150 mg/200  g/day for 30 days). The estradiol test used the Enzyme-linked immunosorbent assay (ELISA). Anova's test results with a significant degree of α = 0.05 established that the p-value of 0.000 was less than α = 0.05, meaning there was a significant effect of giving black soy extract on increased levels of estradiol in the rat blood ovariectomy. Post - Hoc Tukey testing shows increased the highest estradiol levels in the dose treatment group 100 mg/200  g/day. This research proves that the administration of black soy extract (Glycine soja) can increase the levels of estradiol in the rat blood (Rattus norvegicus) ovariectomy.

scholarly journals Effect of Giving Mahkota Dewa Fruits (Phaleria Macrocarpa) Extract to Epithelialization In Incision Wound of White Rats (Rattus Norvegicus)

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Idola Perdana Sulistyoning Suharto
Keyword(s):  
Treatment Group ◽  
Rattus Norvegicus ◽  
Male Rats ◽  
Control Group ◽  
P Value ◽  
Control Group Design ◽  
White Rats ◽  
Significant Difference ◽  
Phaleria Macrocarpa ◽  
Incision Wound

The research purpose was to analysis effect of giving mahkota dewa fruits (Phaleria macrocarpa) extract to epithelialization in incision wound of white rats (Rattus norvegicus). The method was randomized posted-only control group design. There were 30 male rats (Rattus norvegicus) grouped on control and treatment group. Control group divided into three groups (KK1, KK2, KK3) and also treatment group divided into three groups (KP1, KP2, KP3). Control group just given CMC 1% peroral without mahkota dewa fruits extract, the treatment group given mahkota dewa fruits extract 22.5 mg/kg body weight. The data was analyzed by Kruskall Wallis. Based on Kruskall Wallis test, obtained result that there was a significant difference (p<0.05) epithelialization variable with p value p = 0.000 between control and treatment group. And based on One-way Anova test, obtained result that there was a significant difference (p<0.05) with p value p =0.000 between control and treatment group. The conclusion of this research was giving mahkota dewa fruits (Phaleria macrocarpa) extract can increase epithelialization in incision wound of white rats (Rattus norvegicus). Keywords : Mahkota Dewa Fruits (Phaleria Macrocarpa) Extract, Epithelialization, Incision Wound 

scholarly journals The influence of injection of depot medroxy progesterone acetate (DMPA) to the gland tissue histology breast on white rats breastfeeding

2018 ◽  
Vol 12 (2) ◽  
pp. 94
Author(s):  
Asmita Dahlan
Keyword(s):  
Treatment Group ◽  
Group Treatment ◽  
Female Rats ◽  
White Female ◽  
Control Group ◽  
Control Group Design ◽  
Pathology Laboratory ◽  
White Rats ◽  
Gland Tissue ◽  
Tissue Histology

<p><em>Depo MedroxyProgesteroneAcetate</em> (DMPA) allegedly have the effect of breast glandular tissue breast-feeding women, and to prove it then conducted research into how the influence of injection of DMPA to the gland tissue histology breast on white female rats breastfeeding.This research is experimental research design with post test only control group design that was implemented starting carried out in laboratories of pharmaceutical Faculty of Science and anatomic pathology laboratory andalas university medical school fields, the total sample 27 white female rats lactating drawn at random by age 2-3 months, weight 160-200 grams, divided into 3 groups consisting of the control group, treatment group 1 (DMPA 150 mg) and treatment group 2 (DMPA 300 mg). DMPA injections done in intra muscular  every 5 days during 4x injections (20 days). Research data were statistically processed using anova test with 95% confidence. Results showed that there are differences in the development of lobulus and asinus in the control group and treatment group.Conclusions of this study is the effect of DMPA injections in female rats breastfeeding on the area and the development of lobular breast lobes as well as breast acini.</p>

scholarly journals Pemberian makanan pengganti cair Nutrisure Gold® tidak meningkatkan kadar hormon estrogen tetapi meningkatkan kadar hormon progesteron pada anak tikus putih (Rattus norvegicus) galur Wistar betina

2017 ◽  
Vol 9 (1) ◽  
Author(s):  
Setia Wardani ◽  
Wimpie Pangkahila ◽  
IGM Aman
Keyword(s):  
Treatment Group ◽  
Rattus Norvegicus ◽  
Female Rats ◽  
Progesterone Level ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Estrogen Level ◽  
Post Test ◽  
Female Wistar Rats

Abstract: One of the factors that influences aging process is hormonal imbalance that can occur due to the intake of food or drink containing hormone. Children aged more than 1 year with difficulty in eating are usually given Nutrisure Gold®. Analysis has shown that Nutrisure Gold® contains estrogen dan progesterone. This study was aimed to prove that Nutrisure Gold® could increase estrogen and progesterone levels in Wistar female rats. This was an experimental study with the post test only control group design. Subjects were 36 female Wistar rats (Rattus norvegicus) aged 21 days divide into 2 groups, each of 18 rats. The control group was given white rice 3.11 g and aqua bidest meanwhile the treatment group was given Nutrisure Gold® 0.93 g three times a day for 28 days. The results showed that the average estrogen level in the control group was 44.26±11.49 pg/ml and of the treatment group was 45.30±7.66 pg/ml. The average progesterone level in the control group was 22.43±3.62 ng/ml and of the treatment group was 27.44±2.28 ng/ml. Data of estrogen level were analyzed with t-independent test that showed t = -0.320 and P = 0.751 (P >0.05) meanwhile of progesterone level showed t = -4.978 and P = 0.000 (P <0.05). Conclusion: After treatment, the average estrogen levels in both groups did not differ significantly meanwhile the average progesterone levels in both groups showed significant difference.Keywords: liquid replacement meal, estrogen, progesteroneAbstrak: Salah satu faktor yang memengaruhi proses penuaan ialah ketidak seimbangan hormon yang dapat terjadi antara lain akibat pemberian asupan makanan atau minuman yang mengandung hormon. Anak-anak berusia satu tahun ke atas dengan masalah sulit makan sering diberikan makanan pengganti cair Nutrisure Gold®. Analisis menunjukkan bahwa Nutrisure Gold® mengandung hormon estrogen dan progesteron. Penelitian ini bertujuan untuk membuktikan bahwa pemberian Nutrisure Gold® meningkatkan kadar hormon estrogen dan progesteron pada tikus putih galur Wistar betina umur 21 hari. Jenis penelitian ialah eksperimental dengan post test only control group design, Subyek penelitian ialah 36 ekor tikus putih (Rattus norvegicus) galur Wistar betina umur 21 hari yang dibagi atas 2 kelompok, masing-masing kelompok terdiri dari 18 ekor. Kelompok kontrol diberikan nasi putih 3,11 gr dan aquabides sedangkan kelompok perlakuan diberikan Nutrisure Gold® 0,93 gr 3 kali sehari selama 28 hari. Hasil penelitian menunjukkan rerata kadar estrogen kelompok kontrol 44,26±11,49 pg/ml dan rerata kelompok perlakuan 45,30±7,66 pg/ml. Rerata kadar progesteron kelompok kontrol 22,43±3,62 ng/ml dan rerata kelompok perlakuan 27,44±2,28 ng/ml. Analisis kemaknaan kadar estrogen dengan uji t-independent menunjukkan t = -0.320 dan p = 0,751 (P >0,05). Analisis kemaknaan kadar progesteron dengan uji t independent menunjukkan nilai t = -4,978 dan p = 0.000 (P < 0,05). Simpulan: Setelah diberi perlakuan, rerata kadar estrogen pada kedua kelompok tidak berbeda bermakna tetapi rerata kadar progesteron pada kedua kelompok menunjukkan perbedaan bermakna.Kata kunci: makanan pengganti cair, estrogen, progesteron

scholarly journals Nigella sativa EXTRACT IMPROVES SEMINIFEROUS TUBULE EPITHELIAL THICKNESS IN LEAD ACETATE-EXPOSED BALB/C MICE

2017 ◽  
Vol 53 (3) ◽  
pp. 180
Author(s):  
Alis Nur Diana ◽  
Reny I’tishom ◽  
Sri Agus Sudjarwo
Keyword(s):  
Treatment Group ◽  
Mus Musculus ◽  
Group Treatment ◽  
Lead Acetate ◽  
Nigella Sativa ◽  
The Body ◽  
Control Group ◽  
Tubular Epithelium ◽  
Control Group Design ◽  
Black Cumin

Lead that enters the body may lead to increased production of ROS (Reactive Oxygen Species) that may affect reproductive system. Black cumin (Nigella sativa) extract contains high antioxidant, tymoquinone, that may be used to suppress oxidative stress induced by lead in animal experiments. This study aimed to prove that black cumin (Nigella sativa) extract improves the thickness of seminiferous tubular epithelium in Balb/c mice exposed to lead (Pb) acetate. This study used post-test only control group design. The subjects in this study were 30 Balb/c male mice (Mus musculus), divided into 5 groups. K-group: control group without lead acetate and black cumin extract, K+ group: group with 50 mg/kgBW of lead acetate for 28 days, P1 group: treatment group with 50 mg/kgBW of lead acetate for 28 days + 0.3 mg/gBW of black cumin extract for 21 days, P2 group: treatment group with 50 mg/kgBW of lead acetate for 28 days + 0.6 mg/gBW of black cumin extract for 21 days, and P3 group: treatment group with 50 mg/gBW of lead acetate for 28 days + 1.2 mg/gBW black cumin extract for 21 days. The results showed that the mean ± standard deviation of the highest thickness of seminiferous tubular epithelium was 45.80 ± 2.73 in the group that was exposed to acetate + 0.6 mg/gBW of black cumin (P2), and the lowest was 32.75 ± 4.07 in the group that was exposed to lead acetate (K+). The results were then analyzed by Anova test. The results showed that there was significant differences in the thickness of seminiferous tubular epithelium between P1, P2, P3 and K+. In conclusion, black cumin extract administration was proved to improve the thickness of seminiferous tubular epithelium in lead (Pb) acetate-exposed Balb/c mice (Mus musculus).

scholarly journals Default sequence of Mozart’s compositions during pregnancy gave higher dendritic density in the cerebrum and cerebellum of Rattus norvegicus offsprings compared with reversed sequence and control

2019 ◽  
Vol 27 (1) ◽  
pp. 5
Author(s):  
Harry Mangasi Binsar Panjaitan ◽  
Hermanto Tri Joewono ◽  
Widjiati Widjiati
Keyword(s):  
Experimental Study ◽  
Treatment Group ◽  
Rattus Norvegicus ◽  
Control Group ◽  
Exposure Group ◽  
Control Group Design ◽  
Group Design ◽  
Significant Difference ◽  
Post Test ◽  
And Control

Objectives: This study aim to determine sequence of Mozart songs in the analyzing differences in dendritic density of cerebellum and cerebellum of new born Rattus novergicusMaterials and Methods: Experimental study randomized post test only control group design using Rattus norvegicus. Animal subjects were divided into three groups which were control group, and the treatment group that were given exposure to default sequence and reversed sequence of Mozart's music from gestation day 10. We used a comparison test in the analysis expression of BDNF.Results: In the cerebrum there was a significant difference in the Mozart group in reverse rather than with Mozart standard sequence and in standard Mozart group with no exposure group, with p=0.003 and p=0.000. In the cerebellum there was a significant difference in the group in reverse rather than with standard Mozart and in the standard Mozart group with the control group, with p=0.000 and p=0.000. However, there was no significant difference between control group and Mozart group upside down in cerebrum and cerebellum with p=0.109 and p=0.077Conclusion: The density of dendrites in the cerebrum and cerebellum of Rattus norvegicus newborn exposed to Mozart's music during pregnancy with standard Mozart sequence was higher than that in those receiving reverse order and without exposure. There were no significant differences between the density of the cerebrum and cerebellum dendrites between groups exposed to reversed sequence of Mozart composition and those without exposure.

scholarly journals Pengaruh pendidikan gizi dalam upaya meningkatkan kepatuhan konsumsi zat besi melalui kelas ibu hamil

2015 ◽  
Vol 12 (2) ◽  
pp. 79
Author(s):  
Siti Sulastijah ◽  
Sumarni DW ◽  
Siti Helmyati
Keyword(s):  
Treatment Group ◽  
Pregnant Women ◽  
Nutrition Education ◽  
Group Treatment ◽  
Iron Supplementation ◽  
Daily Basis ◽  
T Test ◽  
Deficiency Anemia ◽  
Control Group ◽  
Control Group Design

Background: Iron deficiency anemia contributed to a number of 50% in anemia and caused mortality of 841,000 every year in the world. Nutrition education before iron supplementation is needed because of low compliance in pregnant women. Gatak Sub District that is located in Sukoharjo Regency had the highest risk in pregnant women by 20.02%.Objective: This research aimed to know the effect of nutrition education in an effort to increase compliance of  iron consumption through prenatal classes in Gatak Sub district Sukoharjo Regency.Method: The method of this research was quasi experiment with pre test-post test with non equivalent control group design. The samples of this research were 70 pregnant women who fulfilled inclusion and exclusion criteria. A number of 33 pregnant women were in treatment group and 37 pregnant women were in control group. Treatment group received a nutrition education through anemia booklet and iron supplementation which contained 60 mg sulfas ferosus and 400 µg folic acid on a daily basis in prenatal classes for 3 months. Control group did not receive a nutrition education, but still received a daily iron supplementation for 3 months. Independent t-test and paired t-test were used to analyze data.Results: The knowledge level (higher 12.59), compliance of iron consumption (15.16%), food intakes including energy (higher 9.24%), protein (higher 10.98%), and Fe (higher 6.68%), although those food intakes were still below the RDA, and hemoglobin level (higher 0.44 g/dl), in treatment group compared with control (p<0.05). The factors of age, education level, household expenditure, parity, ANC, nutritional status, and last birth spacing did not affect the compliance of iron consumption (p>0.05).Conclusion: The nutrition education affected the increase in compliance of iron consumption through prenatal classes in Gatak Sub District Sukoharjo Regency.

scholarly journals Pengaruh Pemberian Depo Medroxy Progesterone Acetate (DMPA) Jangka Panjang Terhadap Kadar Endotelin 1(ET-1) Pada Tikus Wistar (Rattus norvegicus)

2020 ◽  
Vol 11 ◽  
pp. 78
Author(s):  
Nurlina Akbar ◽  
Nurul Husnah
Keyword(s):  
Treatment Group ◽  
Rattus Norvegicus ◽  
Control Group ◽  
Elisa Method ◽  
Control Group Design ◽  
Endothelin 1 ◽  
Group Design ◽  
High Production ◽  
True Experiment

The long-term use of Depo Medroxy Progesterone Acetate (DMPA) can cause hypertension. Hypertension is often related to high production of endothelin-1 in the blood. Mechanism of DMPA can cause hypertension is still unclear. Therefore, this study aims to find out the effect of long-term use of DMPA on ET-1 production. This research designed was a true experiment with Posttest Only Control Group Design conducted on 12 female wistar (Rattus norvegicus) strain rats. The mice in the control group were not treated while the treatment group was injected with DMPA 2.7 mg/ week for 8 weeks. Endothelin 1 plasma was assessed at 4 and 8 weeks using ELISA method. The result of this research showed that in the 4th week, ET-1 significantly increase (p = 0.003) in the treatment group (130.38 ± 4.03) compared to the control group (121.64 ± 3.76). Whereas at week 8, there was a tendency to increase ET-1 in the treatment group (132.16 ± 8.97) compared to the control group (126.77 ± 18.11), but not significant (p = 0.528). From the results of this study can be concluded that the long-term use of DMPA in female mice can increase plasma levels of endothelin-1. Keywords: longterm DMPA; estrogen decrease; endotheline- 1 ABSTRAK Penggunaan Depo Medroxy Progesterone Acetate (DMPA) dalam jangka panjang diketahui dapat menimbulkan hipertensi. Hipertensi sering dikaitkan dengan tingginya produksi endotelin-1 dalam darah. Mekanisime DMPA dapat menyebabkan hipertensi masih belum jelas. Oleh karena itu, penelitian ini bertujuan untuk melihat pengaruh penggunaan DMPA jangka panjang terhadap produksi ET-1. Penelitian ini merupakan true experiment dengan desain penelitian Posttest Only Control Group Design yang dilakukan pada 12 ekor tikus galur wistar (Rattus Norvegicus) betina. Tikus pada grup kontrol tidak diberi perlakuan sedangkan grup perlakuan diinjeksi DMPA 2,7 mg/minggu selama 8 minggu. Plasma endotelin 1 diperiksa pada minggu ke-4 dan minggu ke-8 dengan metode ELISA. Hasil penelitian menunjukkan pada minggu ke-4, ET-1 mengalami kenaikan (p=0.003) pada grup perlakuan (130.38± 4.03) dibandingkan grup control (121.64 ± 3.76). Sedangkan pada minggu ke-8, terjadi kecederungan peningkatan ET-1 pada grup perlakuan (132.16 ± 8.97) dibandingkan grup kontrol (126.77 ± 18.11), namun tidak signifikan (p=0.528). Dari hasil penelitian tersebut dapat disimpukan bahwa pemberian DMPA jangka panjang pada tikus betina dapat meningkatkan kadar plasma endotelin 1. Kata kunci: DMPA jangka panjang; penurunan estrogen; endotelin 1

scholarly journals The relevance of antral follicle count (AFC) as a marker of ovarian reserve in normal and infertile women in a tertiary care centre in Central India

2018 ◽  
Vol 7 (11) ◽  
pp. 4711
Author(s):  
Prasanta K. Nayak ◽  
Chandrima Mukherjee ◽  
Subarna Mitra
Keyword(s):  
Ovarian Reserve ◽  
Local Population ◽  
Antral Follicle ◽  
Antral Follicle Count ◽  
Control Group ◽  
Antral Follicles ◽  
Infertile Women ◽  
Significant Difference ◽  
Age Range ◽  
And Control

Background: The objective of the present study was to establish the role of AFC as a marker of ovarian reserve in fertility-proven and in sub-fertile Indian women, and to establish the baseline cut-off AFC values for Indian women.Methods: This is an Observational Case-Control type of study. Test subjects (n=30, age range 20-35 years) were selected randomly from all the women coming to our Out-Patient Department with Primary Infertility. Healthy female volunteers (n=30, age range 20-35years) were recruited from the local population. Statistics: Student t test was applied to compare the mean Antral Follicle count between the case and control group. Pearson correlation test was applied to assess the correlation between age and AFC for case and control group.Results: The results of the study show that there is a significant difference between the number of antral follicles between fertile and infertile women (p < 0.001). The baseline cut-off for successful pregnancy was established as an AFC of 12. There exists negative correlation (r = -0.249) between age and AFC case group indicating as the age increases AFC decreases.Conclusions: The findings from this study help in strengthening the idea that AFC serves as a marker for ovarian reserve. Our observation indicates that the number of antral follicles is lower in the subfertile patients as compared to the fertile group (in all age groups), in view of the significantly lower median AFC in women of the former group (P < 0.001).

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