scholarly journals Calretinin expression in molecular subtypes of invasive carcinoma breast

2020 ◽  
Vol 8 (4) ◽  
pp. 1498
Author(s):  
Suryagayathri Venugopal ◽  
Cicy P. J. ◽  
Deepa S. ◽  
Sankar Sundaram
Keyword(s):  
Breast Carcinoma ◽  
Molecular Mechanisms ◽  
Invasive Carcinoma ◽  
Molecular Subtypes ◽  
Molecular Subtype ◽  
Prognostic Significance ◽  
P Value ◽  
Carcinoma Breast ◽  
Low Level ◽  
High Level

Background: Breast cancer is a leading cause of cancer death  in women worldwide. Breast carcinoma is currently managed by assessing clinicopathological features. Elucidation of molecular mechanisms of pathogenesis of breast carcinoma  may lead to the development of new targeted therapies, particularly in triple negative cancers. Literature shows a few studies on the expression of calretinin  in breast carcinoma particularly in basal like type and its prognostic significance.  In this study, authors are trying  to assess the expression of  a new marker calretinin in different molecular subtypes of invasive carcinoma breast.Methods: This study was done in  107 cases of invasive carcinoma breast specimens received in  Department of Pathology, Government Medical college, Kottayam from December  2017 to May 2019.Results: Among the molecular subtypes, Basal like tumours showed 68.4% of cases with high level and 31.6% of cases with low level calretinin expression which is comparable with the study by Farrag et al. All the other molecular subgroups showed predominantly low level of calretinin expression.Conclusions: Different molecular subtypes of invasive carcinoma breast showed varied calretinin expression. High level calretinin expression was significantly associated with grade 3 (p value = 0.002), ER negativity (p = 0.004), PR negativity (p = 0.018)  and Basal like molecular subtype (p : <0.001). This suggests that calretinin might play a role in pathogenesis of basal like breast carcinomas.

scholarly journals Molecular profiling of breast carcinoma with IHC surrogates in a tertiary care centre in South India

Biomedicine ◽  
2021 ◽  
Vol 41 (1) ◽  
pp. 75-81
Author(s):  
N. Priyathersini ◽  
J. Thanka ◽  
B Jayashree
Keyword(s):  
Breast Cancer ◽  
Breast Carcinoma ◽  
Triple Negative ◽  
Ductal Carcinoma ◽  
Molecular Subtypes ◽  
Molecular Subtype ◽  
Tertiary Care ◽  
P Value ◽  
Her2 Receptor ◽  
Luminal A

Introduction and Aim: Breast cancer is the most common malignancy in females worldwide. Almost 1.4 million new cases have been diagnosed with breast cancer every year. This aims to study the clinicopathological profile and molecular subtypes of invasive breast carcinoma in resected mastectomy specimens over a period of 5 years. Materials and Methods:A retrospective study of 90 mastectomy and wide local resection specimens received during the period of January 2012 to June 2017 were analyzed. The clinical data of patients including age, gender, and stage of the diseasewere obtained from the medical records section. Immunohistochemical staining for Estrogen Receptor [ER], Progesterone Receptor[PR] and Human Epidermal Growth Factor Receptor 2HER2neu were done.The cases were classified according to the molecular classification based on the ER, PR and HER2 receptor status. Results: The peak incidence of breast carcinoma was in the age group 50 to 60 years. Invasive ductal carcinoma,Not otherwise specified[NOS] accounted for the most common histologic type. There was higher incidence of pT2 tumors in our study. The most common molecular subtype was luminal A, followed by triple negative tumors. These molecular subtypes associated well with Tumor grade and HGDCIS with a statistically significant p value of 0.001 and 0.015 respectively. An increased proportion of Grade 3 tumors were Triple Negative tumors. Conclusion:In breast carcinomas the routine histopathological features provide inexpensive method for understanding tumour biology and prognosis. It`s essential in areas with poor resources. ER, PR and HER2 assessment helps in identifying hormonal status and enables for hormone therapy and anti HER2 therapy.  

scholarly journals Expression of Calretinin in Carcinoma Breast in Correlation with Various Histological and Molecular Subtypes

2021 ◽  
pp. 1-11
Author(s):  
M. B. Sri Hansini ◽  
Hemalatha Ganapathy
Keyword(s):  
Molecular Subtypes ◽  
Molecular Subtype ◽  
Prognostic Significance ◽  
Clinicopathological Parameters ◽  
High Grade ◽  
Proliferative Index ◽  
Carcinoma Breast ◽  
Luminal B ◽  
Grade Ii ◽  
High Proliferative Index

Breast carcinoma is the second most common cancer following lung cancer. The incidence of cancer accounts for 24.2% and cancer death is about 15% among females. There are various prognostic and predictive factors for assessing the patient encountering carcinoma breast. Among the various prognostic factors, histological subtype and molecular subtype play a key role. The calretinin expression in carcinoma breast was seen in high-grade breast carcinomas and basal like subtype of carcinoma breast. Calretinin expression was predominantly noted in high grade II and III carcinomas as in other studies and other histological subtypes like metastatic carcinomas which are considered high grade tumours. The present study aimed to correlate the various clinicopathological parameters with the calretinin expression. The expression of Calretinin was seen mainly in grade II and grade III IDC- NST and metaplastic carcinomas. Among the molecular subtypes, the Basal like subtype and the Luminal B subtype showed calretinin expression. The calretinin expression was associated with tumours with a high proliferative index with Ki67 index >14%. This implies the importance of prognostic significance of calretinin expression, as cases with a high proliferative index are likely to have poor prognosis.

Biological mechanisms that trigger breast cancer (BC) tumor progression are molecular subtype dependent

2007 ◽  
Vol 25 (18_suppl) ◽  
pp. 10581-10581 ◽  
Author(s):  
C. Sotiriou ◽  
C. Desmedt ◽  
B. Haibe-Kains ◽  
A. Harris ◽  
D. Larsimont ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Immune Response ◽  
Clinical Outcome ◽  
Tumor Size ◽  
Tumor Invasion ◽  
Molecular Mechanisms ◽  
Molecular Subtypes ◽  
Proliferation Index ◽  
P Value ◽  
Prognostic Impact

10581 Background: We have recently developed several gene expression indices related to hallmarks of breast cancer involving various biological processes such as tumor invasion, impairment of immune response, sustained angiogenesis, evasion of apoptosis and self- sufficiency in growth signal, and investigated their impact on clinical outcome. Here, we aim to refine our biological understanding and the prognostic impact of these indices according to the previously described molecular subtypes based on the estrogen (ER) and ERBB2 receptors. Methods: Each of these indices were developed in a series of 581 BC samples and then computed on several publicly available microarray studies totaling over 2100 BC patients. Multivariate analyses were used to study the dependency patterns between these indices, the molecular subtypes and their impact on survival. Results: ER-/ERBB2- and ERBB2+ subgroups were significantly associated with high expression levels of the proliferation, tumor invasion, angiogenesis and immune response indices. Multivariate analysis showed that in the ER+/ERBB2- subgroup, only tumor size and the proliferation and tumor invasion indices were significantly associated with clinical outcome, with the proliferation index having the largest HR and most significant p-value (HR 3.25; CI 2.31–4.56; p=1.2 10-11). In contrast, in the ER-/ERBB2- subgroup, only tumor size (HR 2.08; CI 1.14–3.81; p=0.01) and immune response index (HR 0.66; CI 0.46–0.95; p=0.02) were associated with prognosis whereas in the ERBB2+ tumors only nodal status (HR 3.40; CI 0.96–12.10; p=0.05) and tumor invasion index (HR 3.03; CI 1.32–6.95; p=0.009) showed significant association with survival. Of interest, proliferation index lost its significance as almost all ER- /ERBB2- and ERBB2 + tumors showed high proliferation levels. Conclusions: Although proliferation seems to be the strongest parameter predicting clinical outcome in ER+/ERBB2- subtype, immune response and tumor invasion appear to be the main molecular mechanisms associated with prognosis in the ER-/ERBB2- and ERBB2+ subgroups respectively. Defining these clinico-genomic models in the specific molecular subgroups will be the key to success for personalized medicine. No significant financial relationships to disclose.

scholarly journals Hubungan Pengetahuan Sikap Dan Dukungan Dengan Tingkat Kecemasan Remaja Putri Menghadapi Menarche

2021 ◽  
Vol 7 (01) ◽  
pp. 43-55
Author(s):  
Andriani Andriani
Keyword(s):  
Family Support ◽  
Random Sampling ◽  
Emotional Reactivity ◽  
Simple Random Sampling ◽  
Anxiety Level ◽  
P Value ◽  
Chi Square ◽  
Low Level ◽  
High Level ◽  
Relationship Of

Background: Menarche in young women can cause anxiety. Many teenagers view menarche as a frightening thing, because menarche will cause discomfort, pain, dizziness and so on, Psychological symptoms to reject the physiological process. Anxiety is an excessive emotional reactivity, a dull depression, or a sensitive context, an emotional response. Purpose: The objective of the study was to understand the relationship of attitude knowledge and family support with the level of anxiety of adolescent girls. Methods: This research is quantitative type with anlitik design with coss sectional approach. The study was conducted in February - April 2018. The sample in this study was female teenager amounting to 25 people. The sampling technique used is simple random sampling. The study was conducted using primary data obtained from the questionnaire, and univariate and bivariate analysis using chi-square statistical test. Results: From the result of research analysis of knowledge relationship with anxiety level got 15 respondents who have high knowledge of 8 people (53,3%) with high level of anxiety and 7 people (46,70%) with low level of anxiety. 13 respondents were positive attitude 6 people (46,2%) with high level of anxiety and 7 people (53,8%) with low level of anxiety. of 14 respondents who received family support and 7 people (46.2%) with high anxiety level and 7 people (53.8%) with low anxiety level. Conclusion: Based on the results of the study found no relationship of knowledge with anxiety level with p value 0.742> ? 0.05, Based on the results of the study found no relation attitude with anxiety level with p value 0,529> ? 0.05, Based on the results obtained did not there is a relationship of family support with anxiety level with p value 0,495> ? 0,05. Latar Belakang: Menarche yaitu haid pertama kali pada remaja putri dapat menimbulkan kecemasan. Banyak remaja memandang menarche adalah hal yang menakutkan, karena menarche akan menimbulkan ketidaknyamanan, sakit, pusing dan sebagainya, Gejala psikologis untuk menolak proses fisiologis tersebut. Kecemasan  merupakan  reaktivitas  emosional  berlebihan, depresi  yang  tumpul,  atau  konteks  sensitif,  respon  emosional. Tujuan: Tujuan penelitian adalah untuk mengetuhui hubungan pengetahuan sikap dan dukungan keluarga dengan tingkat kecemasan remaja putri. Metode: Penelitian ini berjenis kuantitatif dengan desain analitik dengan pendekatan cross sectional. Penelitian dilakukan pada bulan Februari – April 2018. sampel dalam penelitian ini adalah remaja putri yang berjumlah 25 orang. Teknik sampling yang digunakan adalah simple Random sampling. Penelitian dilakukan menggunakan data primer yang diperoleh dari kuesioner, dan analisis secara univariat dan bivariat menggunakan uji statistik chi-square. Hasil: Dari hasil analisis penelitian hubungan pengetahuan dengan tingkat kecemasan didapatkan  15 responden yang berpengetahuan tinggi 8 orang (53,3%) dengan tingkat kecemasan yang tinggi dan 7 orang (46,70%) dengan tingkat kecemasan yang rendah. 13 responden yang sikap positif 6 orang (46,2%) dengan tingkat kecemasan yang tinggi dan 7 orang (53,8%) dengan tingkat kecemasan yang rendah. dari 14 responden yang mendapat dukungan keluarga dan 7 orang (46,2%) dengan tingkat kecemasan yang tinggi dan 7 orang (53,8%) dengan tingkat kecemasan yang rendah. Kesimpulan: Berdasarkan hasil dari penelitian didapatkan tidak ada hubungan pengetahuan dengan tingkat kecemasan dengan p value 0,742 > ? 0,05, Berdasarkan hasil dari penelitian didapatkan tidak ada hubungan sikap dengan tingkat kecemasan dengan p value 0,529 > ? 0,05, Berdasarkan hasil dari penelitian didapatkan tidak ada hubungan dukungan keluarga dengan tingkat kecemasan dengan p value 0,495 > ? 0,05.

scholarly journals Expression of Mesothelial Marker Calretinin in Breast Cancer

2019 ◽  
Vol 4 (01) ◽  
Author(s):  
Dr. Kalathingal Kamarunisha Aboobacker ◽  
Dr. Prema Saldanha
Keyword(s):  
Breast Cancer ◽  
Lymph Node ◽  
Breast Carcinoma ◽  
Calcium Binding ◽  
Invasive Carcinoma ◽  
Prognostic Significance ◽  
Immunohistochemical Expression ◽  
Node Negative ◽  
Mastectomy Specimen ◽  
Grade 3

Calretinin (CR) is a calcium binding protein of calmodulin superfamily, a widely used marker for mesothelial differentiation. It is also found to be expressed in breast carcinoma. Breast carcinoma is the leading cause of cancer death in women and despite new approaches and advances, it is still difficult to predict the behaviour of the tumour and its prognosis. So there is a need and struggle to identify new markers for prognosis of breast cancer. The study is aimed at evaluating the frequency of Calretinin expression in breast carcinoma and assessing the characteristics of Calretinin positive tumours. Thirty mastectomy specimen of invasive breast carcinoma were analysed histopathologically and for immunohistochemical expression of ER, PR, HER2/neu and Calretinin. In this study 93.33% (28 cases) were of invasive carcinoma, NST and 6.67% were of other subtypes. Patients less than 40 years of age showed low CR expression and patients over 40 showed high CR expression (p = 0.22). Grade 3 tumours showed high CR, grades 1 and 2 showed low CR expression. Lymph node positive cases showed high CR and lymph node negative cases showed low CR. Tumours less than 5cms in size show low CR expression and those over 5cms showed high CR expression (p = 0.38). Negative ER, PR and HER2/neu showed high CR expression. CR expression was high in 54.5% of basal-like subtype and 66.7% of HER2-enriched.CR expression was low in 50% of luminal cases. High Calretinin expression was seen in grade 3, HER2-enriched and basal-like subtypes of breast cancer which may be of considerable prognostic significance.

scholarly journals HUBUNGAN ANTARA PENGETAHUAN TENTANG PROSES MENUA DENGAN TINGKAT DEPRESI PADA LANSIA DI DESA MAJASTO SUKOHARJO

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Dinar Ariasti ◽  
Joko Sutrisno
Keyword(s):  
Aging Process ◽  
P Value ◽  
Self Awareness ◽  
Chi Square ◽  
Cross Sectional ◽  
Low Level ◽  
Moderate Depression ◽  
Level Of Knowledge ◽  
High Level ◽  
The Village

AbstractThe role of knowledge in dealing with depression is to help individuals recognize theexistence in this world by expanding self-awareness, find himself again and is responsiblefor the direction of his life. Initial survey results indicate that the 3 (three) respondenexperienced the characteristics of someone who is depressed by the low level ofknowledge. The purpose of this study was to determine the relationship of knowledgeabout the aging process with a level of depression in elderly Majasto Village, DistrictTawangsari, Sukoharjo.Subject of this study was elderly people in the village Majasto, based on an initial surveyfound as many as 65 elderly. This research method in this study an analytic correlationwith cross sectional approach. Data collection tool used is a questionnaire to measure thelevel of knowledge of the aging process with depression from Majasto village.Results of the study there were respondents with a high level of knowledge nothing isseverely depressed, two respondents had moderate depression and 10 respondents didnot experience depression and 3 respondents with a low level of knowledge into a deepdepression and 2nd respondents had moderate depression. the results of Chi-Square testwith SPSS version 18.0 with α = 5% (0:05) was obtained p <0.001 to p value of <0.05,which means that Ho refused and Ha accepted. Conclusion of this study there is arelationship between the level of knowledge about the aging process with a level ofdepression in residents in the village Majasto Tawangsari Sukoharjo.Keywords: knowledge, depression

ColotypeR: A tool to classify colon cancers by consensus molecular subtype and subtype-specific risk of recurrence.

2018 ◽  
Vol 36 (4_suppl) ◽  
pp. 632-632
Author(s):  
Steven Allen Buechler ◽  
Sunil S. Badve ◽  
Yesim Gokmen-Polar ◽  
Emily Herring ◽  
Katelyn Ludwig ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Molecular Subtypes ◽  
Molecular Subtype ◽  
Prognostic Significance ◽  
Response To Treatment ◽  
Training Set ◽  
Risk Of Recurrence ◽  
Molecular Features ◽  
Colon Cancers ◽  
Specific Risk

632 Background: Colon cancer is highly heterogeneous in prognosis and response to treatment. The consensus molecular subtypes (CMS1-4, and mixed) partition colon cancers into distinct groups. CMS4 tumors, a mesenchymal subtype, have the worst prognosis and poor response to standard chemotherapies. There is a critical need for accurate molecular subtyping, and subtype-specific management. Methods: Affymetrix microarrays colon cancer datasets (N = 813; GSE39582, GSE14333) were partitioned into training (AT; N = 370) and validation sets (AV; N = 443) balanced for clinical traits. A novel multistate gene methodology was used to predict CMS, and prognosticate subtype-specific relapse-free survival (RFS) in the training set. Accuracy of CMS prediction and prognostic significance was validated in the AV and TCGA colon cancer (COAD; N = 458) sets. Results: In the training set, a 20-gene panel (ColotypeR-CMS) predicts CMS subtype. Mean accuracy for CMS1-4 prediction was 0.87 in AV and 0.81 in COAD. In AV, 5-year RFS is 0.52 (95%CI 0.43 – 0.63) in the predicted CMS4, and 0.70 (95%CI 0.65 – 0.77) in the non-CMS4 samples. The risk of relapse for non-CMS4 samples was refined by a genomic score (ColotypeR-Risk) computed using expression of 25 genes in the training set. ColotypeR-Risk was prognostic of RFS (p = 0.0004) among the AV non-CMS4 samples, and also prognostic (p = 0.0001) among the stage II non-CMS4 AV samples not treated with chemotherapy. The prognostic significance of ColotypeR-Risk among non-CMS4 samples is independent of tumor location (right or left) and CMS subtype (CMS1-3 or mixed). ColotypeR-Risk was also prognostic of RFS in non-CMS4 samples in COAD (p = 0.005). Conclusions: ColotypeR identifies the consensus molecular subtypes (CMS1, CMS2, CMS3, CMS4, or mixed), and assesses subtype-specific risk of recurrence of colon cancer. ColotypeR identifies prognostic risk and molecular features that could help guide the management of colon cancer patients.

scholarly journals Adult diffuse glioma GWAS by molecular subtype identifies variants in D2HGDH and FAM20C

2020 ◽  
Vol 22 (11) ◽  
pp. 1602-1613
Author(s):  
Jeanette E Eckel-Passow ◽  
Kristen L Drucker ◽  
Thomas M Kollmeyer ◽  
Matt L Kosel ◽  
Paul A Decker ◽  
...  
Keyword(s):  
Genome Wide Association Study ◽  
Molecular Subtypes ◽  
Molecular Subtype ◽  
P Value ◽  
Diffuse Glioma ◽  
Idh Mutation ◽  
Germline Variants ◽  
Genome Wide ◽  
A Genome ◽  
Tert Mutation

Abstract Background Twenty-five germline variants are associated with adult diffuse glioma, and some of these variants have been shown to be associated with particular subtypes of glioma. We hypothesized that additional germline variants could be identified if a genome-wide association study (GWAS) were performed by molecular subtype. Methods A total of 1320 glioma cases and 1889 controls were used in the discovery set and 799 glioma cases and 808 controls in the validation set. Glioma cases were classified into molecular subtypes based on combinations of isocitrate dehydrogenase (IDH) mutation, telomerase reverse transcriptase (TERT) promoter mutation, and 1p/19q codeletion. Logistic regression was applied to the discovery and validation sets to test for associations of variants with each of the subtypes. A meta-analysis was subsequently performed using a genome-wide P-value threshold of 5 × 10−8. Results Nine variants in or near D-2-hydroxyglutarate dehydrogenase (D2HGDH) on chromosome 2 were genome-wide significant in IDH-mutated glioma (most significant was rs5839764, meta P = 2.82 × 10−10). Further stratifying by 1p/19q codeletion status, one variant in D2HGDH was genome-wide significant in IDH-mutated non-codeleted glioma (rs1106639, meta P = 4.96 × 10−8). Further stratifying by TERT mutation, one variant near FAM20C (family with sequence similarity 20, member C) on chromosome 7 was genome-wide significant in gliomas that have IDH mutation, TERT mutation, and 1p/19q codeletion (rs111976262, meta P = 9.56 × 10−9). Thirty-six variants in or near GMEB2 on chromosome 20 near regulator of telomere elongation helicase 1 (RTEL1) were genome-wide significant in IDH wild-type glioma (most significant was rs4809313, meta P = 2.60 × 10−10). Conclusions Performing a GWAS by molecular subtype identified 2 new regions and a candidate independent region near RTEL1, which were associated with specific glioma molecular subtypes.

scholarly journals Clinical and imaging presentation of molecular types of breast cancer

Mastology ◽  
2020 ◽  
Vol 30 (Suppl 1) ◽  
Author(s):  
Vanessa Monteiro Sanvido ◽  
Morgana Domingues da Silva ◽  
Patricia Zaideman Charf ◽  
Simone Elias ◽  
Afonso Celso Pinto Nazário
Keyword(s):  
Breast Cancer ◽  
Breast Carcinoma ◽  
Molecular Subtypes ◽  
Molecular Subtype ◽  
Histological Grade ◽  
Malignant Neoplasm ◽  
Sao Paulo ◽  
Her2 Overexpression ◽  
São Paulo ◽  
Ki 67

Introduction: Breast cancer is the second most common malignant neoplasm among women in Brazil and worldwide. Its incidence increases with age, especially in individuals older than 50 years. Mammography is the main screening test, has high sensitivity, and is the only method that has made an impact on mortality rate. Breast cancer is classified into molecular subtypes, based on immunohistochemical markers. The luminal A subtype (LA) presents estrogen receptor (ER) and progesterone receptor (PR) positive, HER2 negative, and low Ki-67 index. Luminal B (LB) shows ER and/or PR positive, HER2 negative, high Ki-67 index, or HER2 positive (luminal HER2). HER2 has HER2 overexpression and ER and PR negative. Triple-negative (TN) has ER, PR, and HER2 negative and high histological grade. Objective: To evaluate patient characteristics according to the molecular subtypes of breast carcinoma among individuals treated at the Hospital São Paulo – Universidade Federal de São Paulo. Method: This is a retrospective study based on the analysis of medical records of breast cancer cases from the Hospital São Paulo between 2013 and 2016. During this period, 235 patients were treated. Among them, 40% were classified as LA, 34% as LB, 8% as luminal HER2, 15% as TN, and 3% as HER2. The mean age was 57.6 years. The incidence of breast carcinoma was higher in women over 50 years of age in all subtypes: 75.2% for LA, 65% for LB, 58% for luminal HER2, 100% for HER2 overexpression, and 75.1% for TN. Regarding ethnicity, most women were white in all subtypes, accounting for 66.5% of cases. In all subtypes, the most common clinical complaint was nodule: 86% for LA, 86% for LB, 100% for HER2 overexpression, and 96% for TN. Among the mammographic findings, nodule was the most frequent in all subtypes. Luminal subtypes presented other findings, such as suspicious calcifications (14% for LA and 21% for LB), focal asymmetries (14% for LA and 5% for LB), and distortions (2% for LA and 3% for LB). Conclusion: Breast cancer has a higher incidence among Caucasian individuals and those aged 50 to 60 years. The clinical and imaging presentation of tumors is influenced by their molecular subtype: luminal subtypes have a greater diversity of findings and non-palpable lesions, while TN tumors usually manifest as palpable nodules.

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