A study of rotavirus infection in acute diarrhoea in children less than 5 years of age

: Diarrhoeal diseases account for an estimated 1.5 million deaths globally every year making it the second leading cause of childhood mortality. In India 1 out of every 250 children die of rotavirus diarrhea each year.: To find out the incidence of rotavirus infection in acute diarrhoeal cases in children under 5 years of age.: A prospective study was conducted on 100 non repetetive stool samples of Children under 5 years of age, presenting with acute diarrhea and hospitalized in the pediatric ward, during December 2015 to November 2016. Stool samples were processed according to premier rotaclone enzyme immunoassay protocol for the detection of rotavirus antigen, adhering to standard laboratory precautions.: The incidence of acute diarrhoeal diseases was 5.86% in our setting. was detected in 29% cases by ELISA method.The antigen detection by EIA is a reliable test, as it is quantitative and also has high sensitivity and specificity. Hence, can be routinely employed to prevent major morbidity and mortality among children, especially less than 5 years of age.

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Rotavirus serotypes and electropherotypes identified among hospitalised children in São Luís, Maranhão, Brazil

Revista do Instituto de Medicina Tropical de São Paulo ◽

10.1590/s0036-46652005000500009 ◽

2005 ◽

Vol 47 (5) ◽

pp. 287-293 ◽

Cited By ~ 13

Author(s):

Cláudia Regina N. E. Luz ◽

Joana D'Arc P. Mascarenhas ◽

Yvone B. Gabbay ◽

Ana Regina B. Motta ◽

Telma Vitorina Ribeiro Lima ◽

...

Keyword(s):

Acute Diarrhoea ◽

Incidence Rates ◽

Northeastern Brazil ◽

Rotavirus Infection ◽

First Semester ◽

Rotavirus Gastroenteritis ◽

Positive Stool ◽

Stool Samples ◽

Rotavirus Infections

During June 1997-June 1999 rotavirus infection was screened in infants aged up to 2 years and hospitalised with acute diarrhoea in São Luís, Northeastern Brazil. Altogether, 128 stool samples were collected from diarrhoeic patients and additional 122 faecal specimens from age- and- temporal matched inpatients without diarrhoea were obtained; rotavirus positivity rates for these groups were 32.0% (41/128) and 9.8% (12/122), respectively (p < 0.001). Both electropherotyping and serotyping could be performed in 42 (79.2%) of the 53 rotavirus-positive stool samples. Long and short electropherotypes were detected at similar rates - 38.1% and 40.5% of specimens, respectively. Overall, a G serotype could be assigned for 35 (83.3%) of specimens, the majority of them (66.7%) bearing G1-serotype specificity. Taking both electropherotypes and serotypes together, G1 rotavirus strains displaying long and short RNA patterns accounted for 30.9% and 19.0% of tested specimens, respectively; all G2 strains had short electropherotype. Rotavirus gastroenteritis was detected year-round and, in 1998, the incidence rates tended to be higher during the second semester than in the first semester: 45.2% and 26.1% (p = 0.13), respectively. Rotavirus infections peaked at the second semester of life with frequencies of 30.1% and 13.5% for diarrhoeic children and controls, respectively. While the six rotavirus strains bearing G2-type specificity were circulating throughout the whole study period, G1 serotypes (n = 27) emerged as from June 1998 onwards, 20 (74.1%) of which clustering in 1998. These data underscore the importance of rotaviruses in the aetiology of severe infantile gastroenteritis in Northeastern Brazil and sustain the concept that a future vaccine should confer protection against more than one serotype.

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Rotavirus Disease Burden and Molecular Epidemiology in Children with Acute Diarrhoea Age Less than 5 Years in Nepal

Journal of Nepal Paediatric Society ◽

10.3126/jnps.v31i3.5360 ◽

1970 ◽

Vol 31 (3) ◽

pp. 209-215 ◽

Cited By ~ 5

Author(s):

JB Sherchand ◽

NA Cuncliffe ◽

S Tandukar ◽

M Yookoo ◽

BD Pandey ◽

...

Keyword(s):

Disease Burden ◽

Acute Diarrhoea ◽

Watery Diarrhea ◽

National Immunization ◽

Acute Watery Diarrhea ◽

High Prevalence ◽

Severe Gastroenteritis ◽

Stool Samples ◽

Immuno Assay ◽

Rotavirus Diarrhea

Introduction: Rotavirus is the most common cause of severe gastroenteritis in infants and young children worldwide. The aim of the study was to determine rotavirus disease burden and distribution of rotavirus genotypes in children less than five years of age. Methodology: Stool samples were collected from 1768 hospital admitted children under 5 years of age with acute watery diarrhea during November 2005 to October 2008. Rotavirus in stool samples was detected by Enzyme Immuno Assay (EIA) and positive specimens for rotavirus positive was genotyped by reverse transcription-polymerase chain reaction (RTPCR) and sequencing methods. Results: The prevalence of rotavirus was 36.59% (647/1768). Detection was higher in male (33.3%) than female (26.9%). The prevalence was higher during the month of January (2005-06) and February in other periods. During 2005-06, high prevalence of rotavirus was noted in children of age between 6-11 months while in 2006-07 and 2007-08 high prevalence was seen in age group 12-23 months. The most common genotyping of rotavirus identified 5 G types and 3 P types. Genotypes G12 and P [8] were most common during both periods studied (G12; 50%, 29% 33.7% in 2005-06, 2006-07 and 2007-08 respectively and P[8]; 47%, 35% and 43.5% in 2005-06, 2006-07 and 2007-08 respectively). Among six combined genotypes, G12P [6] was most prevalent (34%, 24% and 47.5% in 2005-06, 2006-07 and 2007-08 respectively) where as G1P [6] (4%) was seen in year 2007- 08 only. Conclusions: The study demonstrates the burden of rotavirus diarrhea in Nepal. The data on rotavirus genotypes will help inform decisions as to whether rotavirus vaccine should be considered for inclusion in to Nepal’s National Immunization Program. Key words: Rotavirus; Diarrhea; Genotyping; Children DOI: http://dx.doi.org/10.3126/jnps.v31i3.5360 J Nep Paedtr Soc 2011;31(3): 209-215

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Rotavirus Nosocomial Infection in Children under 5 years of age: A Preliminary study in Nepal

Journal of Nepal Paediatric Society ◽

10.3126/jnps.v31i1.3540 ◽

2011 ◽

Vol 31 (1) ◽

pp. 30-34 ◽

Cited By ~ 2

Author(s):

JB Sherchan ◽

H Ohara ◽

K Sherpa ◽

S Sakurada ◽

B Gurung ◽

...

Keyword(s):

Nosocomial Infection ◽

Acute Diarrhoea ◽

Cross Sectional Study ◽

Enteric Pathogens ◽

Sectional Study ◽

Cross Sectional ◽

Rotavirus Infection ◽

Common Causes ◽

Before And After ◽

Stool Samples

Introduction: Rotavirus is one of the most common causes of acute gastroenteritis among infants and young children. The spread of rotavirus infection in pediatric wards can cause acute diarrhoea during hospitalization, and in turn, prolong hospitalization or need, rehospitalization. The aim of the study was to determine the prevalence of nosocomial infection due to rotavirus and other causal organisms among children less than 5 years of age. Methodology: A cross-sectional study between November 2009 and May 2010 was conducted among 96 pediatric patients in children hospital, Nepal. Stool samples were collected during hospitalization and up to 3 days after discharge from the hospital. Rotavirus antigens were detected by EIA and standard microbiological procedure were applied for other enteric pathogens. Results: The prevalence of nosocomial infection due to rotavirus was 30.2% (29/96). Of them, 16 (55.17%) of the 29 children with nosocomial infection during hospitalization and/or up to 72 hours after discharge developed diarrhoea. The prevalence of symptomatic cases of nosocomial infection was 55.17% (16/29), which involved 16.7% (16/96) of the study subjects, showing a prevalence of 44.82% (13/29) for asymptomatic nosocomial infection consisted of 13.54% (13/96) of the total subjects. Eleven of the 16 symptomatic subjects of nosocomial infection were affected by diarrhoea during hospitalization and five subjects after discharge. Bacteria (8.3%) and protozoa (5.2%) agents were also causing nosocomial infection in children. Conclusions: Children attending hospital may asymptomatically carry enteric pathogens and potentially act as source of nosocomial infection. Due to the relatively high frequency of nosocomial infection in the Children Hospital of Nepal, it is necessary to follow strictly hygienic rules such as isolation of patients with diarrhoea and hand-washing before and after the examination of each patient and cleaning the examination instruments after use in each patient. Key words: Diarrhoea; children; Rotavirus infection; Cross-sectional study; Nepal DOI: 10.3126/jnps.v31i1.3540J Nep Paedtr Soc 2010;31(1):30-34

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Socio-demographic Characteristics of Campylobacter jejuni infected Diarrhoeal Patients under 5 Years

Bangladesh Journal of Infectious Diseases ◽

10.3329/bjid.v2i2.32293 ◽

2017 ◽

Vol 2 (2) ◽

pp. 33-36

Author(s):

Nasreen Huda ◽

Sofia Andalib ◽

Md. Abdullah Yusuf

Keyword(s):

Campylobacter Jejuni ◽

Acute Diarrhoea ◽

Lower Class ◽

Campylobacter Coli ◽

Age Group ◽

Isolation Rate ◽

Cross Sectional ◽

Socioeconomic Condition ◽

Stool Samples ◽

Children Under 5

Background: Campylobacter jejuni infected diarrhoea can occur in different socioeconomic condition among the children under 5 years. Objective: The purpose of the present study was to see the socioeconomic condition among the Campylobacter jejuni infected diarrhoea children under 5 years of age. Methodology: This was a cross sectional study conducted in the Department of Microbiology at Banghabanhdhu Sheikh Mujib Medical University (BSMMU), Dhaka from July 2007 to June 2008 for a period of 1(one) year. All the diarrhoeal samples were collected from outdoor unit of ICDDR,B. Children up-to 5 years of age with acute diarrhoea with or without blood and mucous associated with either fever or abdominal pain or both were included as study population. Stool samples were collected from all diarrhoeal children in a sterile collection pot. Detection of Campylobacter antigen was performed from stool samples. The inoculated selective media in the candle jar were incubated at 42°C (microaerophilic condition) for 48 hours which is favorable for the growth of the organism. With the above criteria it is confirmed as Campylobacter, further identification of suspected Campylobacter jejuni and Campylobacter coli was confirmed by standard test. Result: Two hundred and seventeen children up-to 5 years of age who were suffering from acute diarrhoea were studied to assess the rate of infection by Campylobacter jejuni and Campylobacter coli as an enteric pathogen. Among 217 diarrhoeal children, Campylobacter were isolated from 28(12.9%) cases. Isolation rate of Campylobacter was 19.3% in below 1 year of age group and 5.8% was above 1 year of age group (p<0.003). Out of 217 study patients 141 were male and 76 were female and the isolation rate of Campylobacter were 20(14.2%) from male and 8(10.5%) from female respectively (p>0.05). About 50.0% Campylobacter positive patients were belong to lower class and 35.7% were in middle class.Conclusion: In conclusion Campylobacter jejuni infected diarrhoea children under 5 years of age are mostly below 1 year of age with a predominance of male.Bangladesh Journal of Infectious Disease 2015;2(2):33-36

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Wide Variability in the Sensitivity and Specificity of Rotavirus Immunoassay Diagnostic Kits in Practice

The Journal of Infection in Developing Countries ◽

10.3855/jidc.11922 ◽

2021 ◽

Vol 15 (11) ◽

pp. 1701-1707

Author(s):

Rouba Shaker ◽

Ebla Abdalrahman ◽

Zainab Ali ◽

Lina Reslan ◽

Houda Harastani ◽

...

Keyword(s):

South Korea ◽

Sensitivity And Specificity ◽

Gold Standard ◽

Surveillance Study ◽

Rt Pcr ◽

Rotavirus Infection ◽

Diagnostic Kits ◽

Wide Variability ◽

Stool Samples ◽

Children Under 5

Introduction: Most hospitals rely on rapid antigen-detection kits for the diagnosis of rotavirus infection. Several small studies reviewed the sensitivity and specificity of some of these kits. These studies showed discrepancy in results obtained for sensitivity and specificity that varied according to the type of kit used, area of study, and type of test used as standard for diagnosis of rotavirus infection. The objective of the study is to determine the sensitivity and specificity of five commonly used rotavirus immunoassay kits in comparison to RT-PCR as standard. Methodology: Stool samples (N = 1,414) collected from children under 5 years of age hospitalized with gastroenteritis were tested for rotavirus by immunoassay kits and RT-PCR in a prospective hospital-based surveillance study conducted at 7 centers in Lebanon. Concordance and discrepancy between the two methods was used to calculate sensitivity and specificity, using RT-PCR as the “gold standard”. Results: The sensitivity and specificity were respectively 95.08% and 86.62% for the SD Bioline® (Standard Diagnostics, Inc, South Korea) kit calculated on 645 samples, 65.86% and 45.90% for the VIROTECT® (Trinity Biotech, Ireland) kit calculated on 327 samples, 83.9% and 64.2% for the Rota-Strip (C-1001) (Coris Bioconcept, Belgium) calculated on 95 samples, 52.3% and 10.9% for the Acon® (Acon Laboratories, Inc, California, USA) kit calculated on 122 samples, 68.1% and 20% for the VIKIA® Rota-Adéno (Biomerieux, France) kit calculated on 32 samples. Conclusion: A wide discrepancy was detected between the calculated and advertised sensitivity and specificity for most of the kits.

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Updates in the Prevalence of Rotavirus Gastroenteritis in Babylon City

Al Mustansiriyah Journal of Pharmaceutical Sciences ◽

10.32947/ajps.18.2.369 ◽

2018 ◽

pp. 1-12

Keyword(s):

Health Care Providers ◽

Rural Areas ◽

Causative Organism ◽

Care Providers ◽

Sample Collection ◽

Rotavirus Infection ◽

Rotavirus Gastroenteritis ◽

Age Range ◽

Latex Test ◽

Children Under 5

Background: Diarrhea is a main cause of morbidity and mortality in children under 5 years old. Globally it is responsible for approximately four billion cases and three million deaths annually. In developing countries, it causes two million deaths each year. The major causative organism is rotavirus which is responsible for one-third of hospitalizations with approximately 40% mortality. Objectives: To determine the prevalence and demographic characteristics of rotavirus infection in Babylon city, Iraq. Materials and Methods: Fecal samples were taken from children with age range of 6 months to 5 years complained of diarrhea during the period beginning in October 2016 till August 2017. The age, gender, residence, the type of feeding, place of the sample collection and duration of diarrhea were recorded. Specimens were analyzed by Latex test for detection of rotavirus. Results: A total of 349 children presented with diarrhea, the rotavirus antigen was detected in 169 fecal specimens from children with diarrhea (48%). More percentages of positive rotavirus specimens were seen in the 5year of age. No gender differences were observed, meanwhile samples obtained from rural areas and breastfed children showed less rotavirus positive infection. Conclusion: The present study confirms that rotavirus infection is still currently a prevalent gastroenteritis causative agent and required careful clinical attention. Pediatricians and health care providers are needed to be encouraged to take into account the children who at risk for developing rotavirus infection including age, residence and type of feeding.

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Updates in the Prevalence of Rotavirus Gastroenteritis in Babylon City

Al Mustansiriyah Journal of Pharmaceutical Sciences ◽

10.32947/ajps.18.02.369 ◽

2018 ◽

pp. 1-12

Keyword(s):

Health Care Providers ◽

Rural Areas ◽

Causative Organism ◽

Care Providers ◽

Sample Collection ◽

Rotavirus Infection ◽

Rotavirus Gastroenteritis ◽

Age Range ◽

Latex Test ◽

Children Under 5

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Rapid detection of Rotavirus antigen in stool sample of acute diarrheic children

Bangladesh Journal of Medical Microbiology ◽

10.3329/bjmm.v6i1.19354 ◽

2012 ◽

Vol 6 (1) ◽

pp. 11-13

Author(s):

Sushmita Roy ◽

S.M. Shamsuzzaman ◽

K.Z. Mamun

Keyword(s):

Stool Sample ◽

Rapid Detection ◽

Cross Sectional Study ◽

Rapid Diagnosis ◽

Age Group ◽

College Hospital ◽

Cross Sectional ◽

Rotavirus Infection ◽

Medical College ◽

Stool Samples

Rotavirus is one of the leading causes of pediatric diarrhea globally. Accurate and rapid diagnosis of Rotavirus diarrhea should reduce unnecessary use of antibiotics and ultimately reduce drug resistance. Study was designed for rapid diagnosis of Rotavirus antigen in stool sample by ICT (Immunochromatographic test) as well as to observe the seasonal variation of rotavirus infection. This cross sectional study was carried out in the department of Microbiology, Dhaka Medical College from January 2011 to December 2011. Eighty stool samples were collected from Dhaka Shishu Hospital and Dhaka Medical College Hospital. All samples were tested for rotavirus antigen by ICT. Among 80 patients, 42 (52.5%) samples were positive for rotavirus antigen. Among these 42 positive samples, 30 (71.43%) were from 0-12 months of age group, 10 (23.81%) from 13 to 24 months of age group and rest 2 (4.76%) from 25 to 36 months of age group. Rotavirus Ag was detected in stool samples from January to April and another peak episode from October to December. Considering the importance of Rotavirus associated diarrhea, rapid detection of Rotavirus infection in human is substantially needed and should be routinely practiced.DOI: http://dx.doi.org/10.3329/bjmm.v6i1.19354 Bangladesh J Med Microbiol 2012; 06(01): 11-13

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Molecular Detection of Group A Rotavirus in under Five Years Old Children with Acute Diarrhoea Admitted to Yangon Children’s Hospital

Myanmar Health Sciences Research Journal ◽

10.34299/mhsrj.00927 ◽

2019 ◽

Vol 31 (1) ◽

pp. 87-92

Keyword(s):

Acute Diarrhoea ◽

Enzyme Linked Immunosorbent Assay ◽

Viral Gastroenteritis ◽

Cross Sectional ◽

Under Five ◽

Group A Rotavirus ◽

Rotavirus Vaccination ◽

Group A ◽

Stool Samples ◽

Considerable Morbidity

Rotaviruses are regarded as the most common cause of viral gastroenteritis and are responsible for considerable morbidity and mortality among children especially under five years of age worldwide. In developing countries like Myanmar, where diarrhoea is in the priority childhood disease, rotavirus surveillance and detection of rotavirus genotypes are utmost important. A hospital-based, cross-sectional descriptive study was conducted at Yangon Children‟s Hospital among under five children admitted for acute diarrhoea from January to October 2016. This study includes detection of Group A rotavirus antigen by commercial enzyme-linked immunosorbent assay (ELISA) and genotyping by multiplex RT-PCR. From a total of 488 collected samples, rotavirus antigen was detected in 219 samples (45%). Rotavirus diarrhoea was most common among the age of 6-11 months (38.8%) followed by 12-23 months (37.9%). The results showed that boys were more commonly affected than girls. Detection of rotavirus positivity was peak in February (57.6 %). Out of 219 stool samples with positive ELISA result, 40 stool samples with high optical density value were proceeded for further determination of G and P genotypes. Regarding distribution of G genotypes, the most common G genotype was G9 which comprised 45%, and that of P genotype was P[8] which comprised 92.5%. Regarding combination of G and P genotypes, the most frequent combination is G9P[8], and it constituted 42.5%. Untypable genotypes were seen in 30% of G and 2.5% of P typing. As rotavirus infection can be prevented by vaccine, WHO recommended that rotavirus vaccination should be included in national immunization program especially in countries where prevalence of rotavirus is high. The distribution of G and P genotypes is important in consideration of appropriate vaccine in pre-vaccination and evaluation of effectiveness of vaccine in post-vaccination period. Therefore, the information on currently circulating genotypes of rotavirus in this study will serve as valuable data for vaccination programme.

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Anemia predicts poor outcomes of COVID-19 in hospitalized patients: a prospective study in Iran

BMC Infectious Diseases ◽

10.1186/s12879-021-05868-4 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Masood Faghih Dinevari ◽

Mohammad Hossein Somi ◽

Elham Sadeghi Majd ◽

Mahdieh Abbasalizad Farhangi ◽

Zeinab Nikniaz

Keyword(s):

Prospective Study ◽

High Sensitivity ◽

Hospitalized Patients ◽

Icu Admission ◽

Reactive Protein ◽

Hs Crp ◽

Prospective Association ◽

Poor Outcomes ◽

A Prospective Study ◽

Ventilator Requirement

Abstract Background There are limited number of studies with controversial findings regarding the association between anemia at admission and coronavirus disease 2019 (COVID-19) outcomes. Therefore, in this research, we aimed to investigate the prospective association between anemia and COVID-19 outcomes in hospitalized patients in Iran. Methods In this prospective study, the data of 1274 consecutive patients hospitalized due to COVID-19 were statistically analyzed. All biomarkers, including hemoglobin and high-sensitivity C-reactive protein (hs-CRP) levels were measured using standard methods. Anemia was defined as a hemoglobin (Hb) concentration of less than 13 g/dL and 12 g/dL in males and females, respectively. Assessing the association between anemia and COVID-19 survival in hospitalized patients was our primary endpoint. Results The mean age of the participants was 64.43 ± 17.16 years, out of whom 615 (48.27%) were anemic subjects. Patients with anemia were significantly older (P = 0.02) and had a higher frequency of cardiovascular diseases, hypertension, kidney disease, diabetes, and cancer (P < 0.05). The frequency of death (anemic: 23.9% vs. nonanemic: 13.8%), ICU admission (anemic: 27.8% vs. nonanemic:14.71%), and ventilator requirement (anemic: 35.93% vs. nonanemic: 20.63%) were significantly higher in anemic patients than in nonanemic patients (P < 0.001). According to the results of regression analysis, after adjusting for significant covariate in the univariable model, anemia was independently associated with mortality (OR: 1.68, 95% CI: 1.10, 2.57, P = 0.01), ventilator requirement (OR: 1.74, 95% CI: 1.19, 2.54, P = 0.004), and the risk of ICU admission (OR: 2.06, 95% CI: 1.46, 2.90, P < 0.001). Conclusion The prevalence of anemia in hospitalized patients with COVID-19 was high and was associated with poor outcomes of COVID-19.

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