scholarly journals Comparative Pharmacognostical and Phytochemical Study of Cassia auriculata and Cassia fistula

2021 ◽  
Vol 21 (1) ◽  
pp. 77
Author(s):  
Shailesh M. Kewatkar ◽  
Vivek V. Paithankar ◽  
Supriya S. Deshpande ◽  
Shirish P. Jain ◽  
Dheeraj H. Nagore ◽  
...  
Keyword(s):  
Phytochemical Analysis ◽  
Cassia Fistula ◽  
Phytochemical Study ◽  
Cassia Auriculata ◽  
Microscopic Evaluation ◽  
The Family ◽  
Physicochemical Evaluation ◽  
Current Article ◽  
Antioxidant Agent ◽  
Layer Chromatography

<em>Cassia auriculata</em> L. (CAL) popularly identified as Tanner’s Cassia and <em>Cassia fistula</em> L. (CFL) is generally known as Golden Shower. Both plants belong to the Family, Fabaceae. These plants are used in skin disease, as hepatoprotective, as anticancer agent and as antioxidant agent. The intention of current article is to put forward the comparative pharmacognostical analysis of <em>Cassia auriculata</em> and <em>Cassia fistula</em> roots in terms of macroscopic evaluation, microscopic evaluation, physicochemical evaluation, extractive values and phytochemical analysis. Thin Layer Chromatography study was carried out for CAL and CFL and data pertaining to the above cited evaluations were recorded for both, CAL and CFL roots. The present study may help in differentiating among these species and these pharmacognostic parameters may serve as a tool for identification, authentication and standardization of CAL and CFL.

Pharmacognostical Investigation of Trapa natans L Leaves

2021 ◽  
pp. 4680-4684
Author(s):  
Chandana Majee ◽  
Rupa Mazumder ◽  
Alka N. Choudhary
Keyword(s):  
Aquatic Plant ◽  
Single Layer ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Trapa Natans ◽  
Microscopic Evaluation ◽  
Anomocytic Stomata ◽  
Physicochemical Evaluation ◽  
The Right ◽  
Who Guideline

Background: Trapa natans L., is annual aquatic plant generally kwon as Water caltrp, Water chest nut belonging to the Trapaceae or Lytraceae family. Trapa natans L is use for the treatment of wide no of diseases without proper standardization. Objective: To give the right pharmacognostical and photochemical information of the Trapanatan L leaves. In this study pharmacognostical investigation of the fresh leaves and powder drug were done to determine the macroscopical, microscopical, quantitative physicochemical and phytochemical property of the drugs. Method: Macroscopical, quantitative and qualitative microscopy, physicochemical evaluation, extractive value, florescence analysis and phytochemical analysis were done according to the WHO guideline. Result: Macroscopical analysis showed that, leaves are greenish to purplish color, rhomboidal shape; alternate, acute, margin is dentate, pinnate venation. Microscopic evaluation showed that leaf is dorsi ventral in nature, upper layer epidermis cells were covered with cuticle layer. Single layer of barrel shape cell were present bellow the upper epidermis layer. Trichomes are generally multicellular. Anomocytic stomata were observed in upper epidermis. From the experiment it was found that methanolic extract give the highest extractive value. Phytochemical analysis gives the evidence for the presence of carbohydrate, alkaloids, glycoside, steroids, flavonoids, tannin, and triterpenoids. Qualitative phytochemical analysis give the evidence for presence of high amount total phenolic content. Conclusion: Different pharmacognostical parameters assessed in this examination help to detection and standardization of Trapa natans L., leaves.

Phytochemical Study and Screening of Antioxidant, Anti-inflammatory Typhonium Flagelliforme

2021 ◽  
pp. 2686-2690
Author(s):  
Nitin A. Mirgane ◽  
Arun Chandore ◽  
Vitthal Shivankar ◽  
Yashwant Gaikwad ◽  
Gurumeet C. Wadhawa
Keyword(s):  
Biological Properties ◽  
Plant Part ◽  
Inflammatory Activity ◽  
Phytochemical Analysis ◽  
Rare Plant ◽  
Active Constituent ◽  
Phytochemical Study ◽  
The Family ◽  
Stem Extract ◽  
Anti Inflammatory

Typhonium flagelliforme from the family Araceae is very rare plant. It is very good curative and work against various illness and infections. The plant part has large importance in Ayurveda. In this study we use stem and leaves used for phytochemical analysis and their biological properties such as Antioxidant and Anti-inflammatory activity. Known Protocol for the phytochemical analysis, while antioxidant and anti-inflammatory activity was determined using known methods. Both the leaves and stem extract show presence of various important active constituent and shows better activity against the free radicals or antioxidant activity or they can show the anti-inflammatory activity.

scholarly journals Pharmacognostic and Preliminary Phytochemical Evaluation of Leaf of Syzygium Cumini (L.) Skeels

2021 ◽  
Vol 12 (3) ◽  
pp. 684-688
Author(s):  
Shyam Baboo Prasad ◽  
Arun M Gurav ◽  
Goli P Prasad ◽  
Anupam K Mangal ◽  
Srikanth N
Keyword(s):  
Single Layer ◽  
Fluorescence Analysis ◽  
Physicochemical Analysis ◽  
Water Soluble ◽  
Phytochemical Analysis ◽  
Syzygium Cumini ◽  
Lower Epidermis ◽  
Microscopic Evaluation ◽  
Physicochemical Studies ◽  
Physicochemical Evaluation

Syzygium cumini (L.) Skeels (Family: Myricaceae) commonly known as Jamun is a widely used medicinal plant in Ayurveda. In spite of its numerous medicinal attributes, no published work is available on pharmacognostic characterization, physicochemical analysis of its leaves. The measures taken for pharmacognostic characterization were macroscopy, microscopy, powder microscopy, physicochemical evaluation, fluorescence analysis, and preliminary phytochemical screening. The microscopic evaluation showed useful characters for the recognition of leaf of Syzygium cumini (L.) Skeels . Transverse section, showed presence of single layer of wavy epidermal cells with striated cuticle single layer Palisade beneath upper epidermis in lamina region (contains compact elongated cells), Spheraphide in lamina region, collenchyma below the upper epidermis and above the lower epidermis in midrib region, Xylem and phloem in center, Sclerenchyma in between vascular bundle and collenchyma in midrib region. Wavy epidermal cell in the upper epidermis, anisocytic stomata in the lower epidermis, Bordered pitted reticulate vessels, Calcium oxalate crystal sheath in the lamina, fragment of fibers and Spiral vessels were observed in powder microscopy. Physicochemical studies showed total ash (3.1%), acid insoluble ash (0.7%), alcohol soluble extractive values (10.96 %), and water-soluble extractive values (12.32 %). Phytochemical analysis revealed the presence of sugar, lipid, Glycoside, saponins, phenols, flavonoids, tannins, tri-terpenoids and steroids. The study forms the first report on pharmacognostic characters and a physicochemical parameter which could be useful for identification and authentication of the plant.

scholarly journals HPTLC analysis of berberine in stem extracts of Fibraurea darshani

2018 ◽  
Vol 7 (2) ◽  
pp. 2021
Author(s):  
Sheema Dharmapal ◽  
Bindu T.K. ◽  
Elyas K.K.
Keyword(s):  
High Performance ◽  
Uv Light ◽  
Solvent System ◽  
Plant Sample ◽  
Phytochemical Analysis ◽  
Methanolic Extracts ◽  
Uv Reflectance ◽  
The Family ◽  
Rf Values ◽  
Layer Chromatography

The present study is a first report on the phytochemical analysis of the plant Fibraurea darshani which is endemic to Western Ghats. The plant is a woody dioecious climber belonging to the family Menispermaceae. Preliminary phytochemical screening of methanolic extracts of the stem of F. darshani revealed the presence of secondary metabolites like alkaloids, carbohydrates, anthraquiones, terpenoids, flavonoids, phenolics, sterols etc. A simple and reproducible high performance thin layer chromatography was developed to evaluate the presence of berberine in methanol extract of stem of F. darshani. This method involves separation of compounds by HPTLC on pre-coated silica gel 60F 254 plates with a solvent system of Chloroform: Ethyl acetate: Methanol: Formic acid (4:5:4:0.3) and scanned using densitometric scanner in UV reflectance photo mode at 254 and 366nm. The Rf values (0.97) for berberine in the plant sample and the reference standard were found comparable under UV light at 366nm. The HPTLC method developed was simple, accurate and specific.

Pharmacognostic and Phytochemical Analysis of Asystasia Variabilis Trim. - An Extrapharmacopoeal Ayurvedic Medicinal Plant

2018 ◽  
Vol 3 (01) ◽  
Author(s):  
Kavitha . ◽  
Shrikanth P. ◽  
Ravikrishna S. ◽  
Suchithra Prabhu
Keyword(s):  
Transverse Section ◽  
Root Hairs ◽  
Single Layer ◽  
Phytochemical Analysis ◽  
Mesophyll Cells ◽  
Phytochemical Study ◽  
Anatomical Features ◽  
Physico Chemical ◽  
The Family ◽  
Traditional Practitioners

Background - Asystasia Variabilis Trim. also known as Maithaala Kaddi by traditional practitioners of Udupi. Belonging to the family Acanthaceae. Is a semi-scandent herb, and effectively used by the Folklore practitioners for the management of Amlapitta (acid peptic disorder), worm infestations and rheumatism. Aim - To explore the pharmacognostical and preliminary phytochemical parameters of plant to standardize the drug. Materials and Methods - whole mature plant of A.variabilis was collected from Udupi district. Macroscopic, microscopic physico-chemical standards, HPTLC and secondary metabolites screening were scientifically recorded. Results - The pharmacognostical leaf study has shown single layered epidermis throughout midrib and lamina. Anatomical features of stem exhibit single layer of epidermis covered with trichomes. Inner to this, densely arranged collenchyma cells are present. In transverse section of root showed epidermal layer is surrounded by root hairs which are abundant. Powder microscopy characteristics showed the presence of starch in parenchyma region, mesophyll cells with stomata and sclerides were present. The standard out print of the drug is represented by physicochemical standards and HPTLC. Preliminary Phytochemical study shows that it contains Alkaloids, Carbohydrates, Steroids, Tannins and Phenol. Conclusion - Pharmacognostical study carried out on A.variabilis showed quality standards of the drug, with respect to its macroscopy, microscopy, physico-chemical standards and HPTLC.

QUALITATIVE AND QUANTITATIVE PHYTOCHEMICAL SCREENING AND IN VITRO ANTIOXIDANT EVALUATION OF PERGULARIA DAEMIA

2018 ◽  
Vol 24 (2) ◽  
Author(s):  
GITA MISHRA ◽  
HEMESHWER KUMAR CHANDRA ◽  
NISHA SAHU ◽  
SATENDRA KUMAR NIRALA ◽  
MONIKA BHADAURIA
Keyword(s):  
Reducing Sugars ◽  
Antioxidant Properties ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Qualitative And Quantitative ◽  
The Family ◽  
Ethanolic Extracts ◽  
Antioxidant Evaluation ◽  
In Vitro Antioxidant Activity

Pergularia daemia belongs to the family Asclepiadaceae, known to have anticancer, anti-inflammatory activity. Aim of the present study was to evaluate qualitative and quantitative phytochemical and antioxidant properties of ethanolic extracts of leaf, stem and root parts of P. daemia . Preliminary phytochemical analysis and in vitro antioxidant properties were evaluated by standard methods. The qualitative phytochemical analysis of P. daemia showed presence of flavonoids, tannins, alkaloid, phytosterol, carbohydrate, phenol, saponin, glycosides, terpenoids, steroids proteins and reducing sugars. Quantitative analysis showed polyphenol, flavonoid, flavonone, flavone and flavonol in P. daemia leaves, stem and root in considerable quantity. The in vitro antioxidant activity of P. daemia clearly demonstrated that leaf, stem and root parts have prominent antioxidant properties and was effective in scavenging free radicals.

FINGER PRINTING ANALYSIS OF THE PHENOLS FROM HOLOPTELEA INTEGRIFOLIA (ROXB.) PLANT LEAVES USING HPTLC

INDIAN DRUGS ◽  
2017 ◽  
Vol 54 (09) ◽  
pp. 67-71
Author(s):  
R. C. Sutar ◽  
◽  
D. S Musmade
Keyword(s):  
High Performance ◽  
Methanolic Extract ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Plant Leaves ◽  
Methanol Extracts ◽  
Finger Printing ◽  
Rf Values ◽  
Layer Chromatography ◽  
Tungsten Lamp

The present study was conducted to identify the phenols from methanol extracts (MHI) of medicinally and economically useful leaves of Holoptelea integrifolia (Roxb.) plant using High Performance Thin Layer Chromatography (HPLC) technique. Preliminary phytochemical screening was done and HPTLC studies were carried out on CAMAG HPTLC system equipped with Linomat V applicator (Switzerland). Densitometric scanning was performed with Camag TLC scanner IV in the reflectance absorbance mode at 540 nm and operated by Win CATS software (1.4.6 Camag) with the help of tungsten lamp. Preliminary phytochemical screening of methanolic extract of Holoptelea integrifolia showed the presence of steroids, alkaloids, flavonoids, proteins, phenols and carbohydrates. HPT LC finger printing of phenols of methanolic extract of leaf revealed seven polyvalent phytoconstituents (7 peaks) and corresponding ascending order of Rf values in the range of 0.15 to 0.75. From the results of preliminary phytochemical analysis and above Rf values, we have concluded the presence of phenols in methanol extracts.

scholarly journals PHYTOCHEMICAL ANALYSIS AND COMPARATIVE ANTIPARKINSON ACTIVITY OF FOUR SPECIES OF MUCUNA

2018 ◽  
Vol 11 (7) ◽  
pp. 125
Author(s):  
Karunanithi M ◽  
David Raj C ◽  
Brindha P ◽  
Jegadeesan M ◽  
Kavimani S
Keyword(s):  
Mucuna Pruriens ◽  
Control Group ◽  
Phytochemical Analysis ◽  
Tongue Protrusion ◽  
Phytochemical Study ◽  
P Values ◽  
Dose Levels ◽  
Chewing Movement ◽  
Vacuous Chewing ◽  
Seed Extracts

Objective: The aim was to study the antiparkinson activity in the seed extracts of four species of Mucuna.Methods: The hydroalcoholic extracts of seeds of four species of Mucuna were evaluated for antiparkinson activity of after a preliminary phytochemical study. The activity was measured in rats by indirectly measuring the decrease in malondialdehyde level, decrease in tongue protrusion frequency, and reduction in vacuous chewing movement after administering reserpine at the dose of 1 mg/kg. The dose levels of four species of Mucuna seed extract were kept at 100, 200, and 300 mg/Kg.Results: Extracts exhibited potent antiparkinson activity and achieved statistically significant p values compared with control group. The study corroborates and compares all four species of Mucuna. Conclusion: Among the extracts, the highest percentage of antiparkinson activity was recorded for Mucuna pruriens.

scholarly journals Quality Control Assessment of an Ayurvedic Medicine - Durvadi Ghrita

2020 ◽  
Vol 11 (4) ◽  
pp. 754-758
Author(s):  
Sangita Kamaliya ◽  
Vaghela D B ◽  
Harisha C R ◽  
Shukla V J
Keyword(s):  
High Performance ◽  
Pollen Grains ◽  
Acid Value ◽  
Morphological Characters ◽  
Ayurvedic Medicine ◽  
Herbal Drugs ◽  
Herbal Drug ◽  
Saponification Value ◽  
Physicochemical Evaluation ◽  
Layer Chromatography

Background: Durvadi Ghrita is a Sneha Kalpana which is claimed to be effective in Madhumehajanya Timira (Diabetic Retinopathy).  In present study, it has been used for Nasya. Objective: Present study was planned to look out on herbal drugs used in the preparation of Durvadi Ghrita and standardization of drug by pharmacognostical and physicochemical parameters and HPTLC evaluation. Methods: Identification and authentication of all the raw drug was done by pharmacognostical study i.e. morphological characters, organoleptic characters and powder microscopy. Physicochemical evaluation and HPTLC of final product were done. Results: Pharmacognostical study of all the raw drugs of Durvadi Ghrita showed presence of oil globule, prismatic crystals of Durva. Lignified branched trichome, pollen grains of Utpala Kinjalaka. Trichome, border pitted vessels of Manjishtha. Collenchyma cells, border pitted vessel of Elvaluka. Lignified fibres, oil globules of Sita. Pitted fibres, pitted vessels of Usheera. Scalariform vessels, prismatic crystals of Musta. Pitted vessels and lignified fibres, crystal fibres of Chandana. Lignified cork, and stone cells of Padmaka etc. Pharmaceutical evaluation of Durvadi Ghrita showed results Specific Gravity 0.9125, Refractive Index 1.47, Acid Value 0.4608, Iodine Value 11.45 and Saponification Value 128.856. High Performance Thin Layer Chromatography, 12 spots were found at 254 nm and five spots were found at 366 nm. Conclusion: Identification and authentication of herbal drug used in the preparation of Durvadi Ghrita has been done. Pharmacognostical and physicochemical evaluation of prepared drug has been carried out which can be further useful for standardization of Durvadi Ghrita and other clinical researches.

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