scholarly journals Characterization of Irradiation Induced Mutants of Cassava (Manihot esculenta Crantz) Generated from Jame-jame and Adira-4 Genotypes at M1V2 Generation

2017 ◽  
Vol 2 (6) ◽  
pp. 22
Author(s):  
Nurul Khumaida ◽  
Sintho Wahyuning Ardie ◽  
Mira Sri Astuti

Cassava (Manihot esculenta Crantz., Euphorbiaceae) is an important dietary carbohydrate source for approximately 800 million people in the tropics. It is a potential crop for food, feed, and industry. Cassava breeding through conventional approaches are hampered with some limitations which resulted in a low number of superior varieties. The objective of this research was to generate several mutant lines with higher yield and starch content. This research intended to identify the second generation (M1V2) Jame-jame and Adira-4 variant cassava mutants from gamma ray irradiation. Jame-jame is a local genotype from Halmahera (local high yielding genotype, sweet, and potential)  and Adira-4 is an Indonesia national variety of cassava. Mutant diversity appears on the plant height, plant branching, and plant tubers that harvested at 10 months after planting (MAP). Our results showed the positive correlation between plant height, first branching height, tuber weight, number of roots, and number of economic roots. Based on tuber weight and number of economic tuber there were 9 potential mutants (putative).  Based on the stability test, there were 6 potential stable mutants (putative) by first branching height, tuber weight, total number of tubers, and total number of economies tuber, namely: V5D1-(2), V5D1-2(2), V5D1-3(2), V5D1-4(3), V5D2-2(2), and V5D2-6(2). Keywords: local genotype, M1V2, storage root, characters,  stability.

2019 ◽  
Vol 20 (2) ◽  
pp. 75
Author(s):  
Hani Fitriani ◽  
N. Sri Hartati ◽  
Enny Sudarmonowati

Along with the increasing the of the land-use change of the fertile agricultural land in Java, the better awareness has been subjected to the importance of function peat land for agricultural development. Cassava (Manihot esculenta Crantz) is a tuber plant that potentially developed as important carbohydrate source. The objective of this research was to identify superior cassava variety candidate of selected cassava developed by of Research Centre Biotechnology, LIPI which has high adaptation capability and yield in Central Kalimantan peat land area. This research was conducted from July 2010 through May 2011 in Central Kalimantan Province (Kalampangan dan Pulang Pisau in total of area 1.5 and 2.25 ha respectively). There were three genotypes of cassava tested, i.e. Iding (high amylose), Gebang (low amylose), and Menti (high starch) compared to Adira 4 (35 ton/ha) and Darul Hidayah (102.1 ton/ha). The variables observed were plant height (cm) and tuber weight (grams). Observations were conducted at the age of 10 months. Data was analyzed using SPSS version 16.0. The results showed that the plant height and yield were higher in Pulang Pisau than Kalampangan even though the difference is not significant (P ≤ 0,05). Darul Hidayah has highest plant height and yield, 229.8 cm and 2271 g respectively among other genotypes/varieties grown at Pulang Pisau even though the value was not significant, whereas in Kalampangan, Darul Hidayah showed the lowest yield (670 g). Based on the data obtained, the Darul Hidayah variety can be cultivated by farmers in Pulang Pisau peat land to fulfill the availability of superior cassava seedlings for food, feed and industry. Keywords: Peat, tuber yield, plant height, liquid organic fertilizer, yam wood.


2016 ◽  
Vol 10 (1) ◽  
pp. 63-70
Author(s):  
Palupi Puspitorini ◽  
Dyah Pitaloka ◽  
Tri Kurniastuti

This study aims to find out the results of cassava tuber crop UJ5 variety at different harvest ages. Research direction is that cassava varieties have been planted throughout Indonesia as the best producer of cassava varieties. High levels of starch and high HCN content makes this variety was selected by the factory - tapioca factory in Indonesia. Research design used randomized block design with 7 treatments of harvesting (UP) were repeated 3 times. UP6 (harvesting 6 MAP), UP7 (harvesting 7 MAP), UP8 (harvesting 8 MAP), UP9 (harvesting 9 MAP), UP10 (harvesting 10 MAP), UP11 (harvesting 11 MAP), UP12 ( harvesting 12 MAP). The research variables are the fresh weight of tuber, tuber starch content (%), the weight of biomass, harvest index, number of tubers per plant. The results showed that the best harvesting time is UP9 the results did not differ with UP10, UP11 and UP12


Author(s):  
Edmundo Rivera ◽  
Fernando Abruña ◽  
José Rodríguez

Cassava (Manihot esculenta Crantz), one of the major sources of carbohydrates throughout the tropics, was found to be very tolerant to high soil acidity in two Ultisols and one Oxisol. About 85% of maximum yields were obtained when Al saturation of the effective cation exchange capacity of the soil was around 60%, but highest yields were attained at about pH 5.3 with no exchangeable Al. Soil acidity factors did not affect the chemical composition of the cassava leaves, except for Mn, which increased with decreasing pH of the Oxisol. Tolerance of cassava to soil acidity was also confirmed by the fact that yields of 12 commercial varieties were not affected by Al saturation levels varying from 0 to 60% in an Ultisol.


2013 ◽  
Vol 10 (2) ◽  
pp. 217-222 ◽  
Author(s):  
MSA Fakir ◽  
M Jannat ◽  
MG Mostafa ◽  
H Seal

Cassava (Manihot esculenta Crantz) roots (tubers) are used as staple food. Starch extracted from tubers is widely utilized as raw materials in industries. Dry matter (DM) content, starch and flour extraction and proximate composition were investigated in seven cassava accessions (Coc-A1, Kh-A2, Cow-A3, Sa-A4, Me-A5, Va-A6 and Sy-A8.) in 2010- 2011. Leaf DM varied from 20.51% in Me-A5 to 29.01% in Sy-A8; that of stem from 27.24% in Va-A6 to 32.10% (average of Sy-A8, Me-A5 and Sa-A4); and that of tuber from 37.30% in Kh-A2 to 45.26% in Sy-A8. Starch was extracted by blending chopped tuber followed by decantation. Tubers were sliced, sun dried and milled into flour. Tuber starch content (fresh wt. basis) varied between 15.04% in Sy-A8 and 24.97% (average of Coc-A1 and Me-A5); that of peel from 4.54% in Va-A6 to 5.85% in Coc-A1. Crude protein varied from 1.80% (average of Kh-A2, Cow-A3 and Sy-A8) to 4.53% in Va-A6. Crude fiber content varied from 1.95% (average of Sa-A4 and Coc-A1) to 4.27% in Cow-A3. Cyanogens present in cassava plant escape as hydrogen cyanide (HCN) during harvesting and processing. Variation for HCN existed and it was 140.95 mg/kg fresh tuber (average of Sy-A8 and Coc-A1) to 546.0 mg/kg fresh tuber in Va-A6. There was no detectable HCN in the extracted flour and starch. It may be concluded that genetic variation for DM, starch, protein and HCN existed in seven cassava accessions, and Coc-A1 may be a better one due to its lower HCN, higher DM and starch content. DOI: http://dx.doi.org/10.3329/jbau.v10i2.14698 J. Bangladesh Agril. Univ. 10(2): 217-222, 2012


2016 ◽  
Vol 155 (4) ◽  
pp. 569-581 ◽  
Author(s):  
S. SRAPHET ◽  
A. BOONCHANAWIWAT ◽  
T. THANYASIRIWAT ◽  
R. THAIKERT ◽  
S. WHANKAEW ◽  
...  

SUMMARYCassava (Manihot esculenta Crantz) root yield measured as fresh weight (hereafter root yield) is declining in much of Asia and Africa. The current study aimed to identify quantitative trait loci (QTL) underlying both root and starch fresh weights in F1 cassava. Eight QTL were associated with root yield, underlying 12·9–40·0% of the phenotypic variation (PVE). Nine QTL were associated with starch content, underlying 11·3–27·3% of the PVE. Quantitative trait loci were identified from four different environments that encompassed two locations and 3 years. Consistent QTL for root yield, YLD5_R11 and YLD8_L09 on linkage group (LG) 16, were detected across years and locations. Quantitative trait loci for starch content, ST3_R09, ST6_R10 and ST7_R11 on LG 11, were found across 3 years. Co-localization of QTL for both traits with positive correlation was detected between YLD3_R10 and ST5_R10 on LG 9. Candidate genes within the QTL that were consistent across multiple environments were identified based on cassava genome sequences. Genes predicted to encode for glycosyl hydrolases, uridine 5’-diphospho-(UDP)-glucuronosyl transferases and UDP-glucosyl transferases were found among the 44 genes located within the region containing the QTL controlling starch content. Sixteen genes predicted to encode proteins that were possibly associated with root yield were identified. The QTL controlling root yield and starch content in the current study will be useful for molecular breeding of cassava through marker-assisted selection. The identification of candidate genes underlying both traits will be useful both as markers and for gene expression studies.


2019 ◽  
Vol 19 (3) ◽  
pp. 206
Author(s):  
Prasasti Dame Aritonang ◽  
Ardian Ardian ◽  
Kukuh Setiawan

This study aimed to evaluate the effect of paclobutrazol and KOH application through leaf togrowth and production of cassava.  This study was conducted from March 2017 to October 2018 in an Integrated Field Universityof Lampung. The treatmens was arranged factorially (4 x 4) in a complete randomized block design with 4 replications be avowed as group.  The main factor were paclobutrazol consist of P1 = 0 ppm (control, no treatment), P2 = 400 ppm, P3 = 500 ppm and P4 = 600 ppm.  The second factor were KOH consist of K1 = 0% (control, no treatment), K2 =0,5%, K3 = 1% and K4 = 1,5 given a week after paclobutrazol’s application.  This study used cassava cutting varieties of kasesart.  Observation variables were plant height, number of books, number of fresh leaves, fresh weight of leaves, dry weight of leaves, fresh weight of stem, dry weight of stem, fresh weight of tubers, dry weight of tubers.  Data of each treatments were calculated its mean value and tested homogeneity.  Homogeneous data were analyzed variance and followed by the smallest real difference testat level 5%.The study’s result showed that paclobutrazol application had inhibited effect the vegetative growth of plant height, number of nodes, fresh and dry weight of stem cassava plants.  Application of KOH had a significant effect only on number of fresh leave at 13 week after planted.  The interaction of paclobutrazol and KOH treatment had a significant effect on leaf dry weight.   


2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Boniface Oure Obong’o ◽  
George Ayodo ◽  
Fanuel Kawaka ◽  
Morelly Kathy Adalla

Cassava (Manihot esculenta Crantz) is a major source of carbohydrates, calcium, vitamins (B and C), and essential minerals and is the third most important source of calories in the tropics. However, it is not clear if the traditional processing methods expose the products to microbial contamination. This study assessed the levels of fungi and aflatoxin contamination in traditionally processed cassava products (Akuoga and Abeta). A total of 38 samples were collected from the local markets in 7 subcounties in Homa Bay County, Kenya. The levels of aflatoxin were determined using an indirect competitive ELISA protocol. Yeast and mould contamination was determined using ISO 21527-2 method. Mean aflatoxin levels in chopped, fermented, and sun-dried cassava (Akuoga) were 0.36 μg/kg compared to 0.25 μg/kg in chopped and sun-dried (Abeta) products. Aflatoxin contamination was detected in 55% of the samples and ranged from 0–5.33 μg/kg. These levels are within 10 μg/kg recommended by the CODEX STAN 193-1995. Yeast and mould counts in fermented and chopped sun-dried products were 3.16 log Cfu/g and 2.92 log Cfu/g, respectively. The yeast and mould counts were above standards set by East African Standard 739:2010 in 62% (Akuoga) and 58% (Abeta). The most prevalent fungal species were Saccharomyces cerevisiae (68.4%) and Candida rugosa (68%) followed by Candida parapsilosis (18.4%), Candida tropicalis (15.8%), Candida humilis (15.8%), and Aspergillus spp. (5.3%). Aspergillus spp. was the only mycotoxigenic mould isolated from the samples. The study shows that cassava consumers are exposed to the risk of aflatoxin poisoning. The study, therefore, recommends appropriate surveillance to ensure safety standards.


1991 ◽  
Vol 65 (3) ◽  
pp. 365-372 ◽  
Author(s):  
Beryl P. Kamalu

Growing dogs were divided into three groups and were fed on a control (rice) diet, a diet in which cassava (Manihot esculenta Crantz; gari) was used as the carbohydrate source, and the rice diet to which cyanide (equivalent to that present in gari) was added. Each group consumed its diet for 14 weeks, during which plasma thiocyanate concentration and plasma lipase (EC 3.1.1.3) activity were monitored. Plasma free amino acids were determined from pooled samples taken at the end of the experimental period, and the insulin status of the dogs was evaluated using the gluconeogenic index. The dogs were killed and the pancreas examined for histopathology. Dogs fed on both gari diet and the rice+cyanide diet generated significant amounts of thiocyanate when compared with the controls, with the rice+cyanide group having higher plasma thiocyanate than the gari group (P < 0.01). Plasma lipase activity rose significantly at the end of the experimental period in the dogs fed on gari (P < 0.05). Gluconeogenesis from protein was greatest in the dogs fed on gari, five times greater than that in the control dogs, while gluconeogenesis from protein in the dogs fed on rice+cyanide was approximately twice as high as that of the control dogs. Histopathological examination of the pancreas showed haemorrhage, necrosis, fibrosis and atrophy of the acinar tissue and fibrosis of the islets of Langerhans in the dogs fed on gari. The pancreas of the dogs fed on rice+cyanide showed similar lesions but haemorrhage was not prominent and fibrosis was more marked. The present study indicated that a hypoinsulinaemia developed which was more severe in the animals fed on gari than in the dogs fed on the rice+cyanide diet and that the condition was not related to the level of plasma thiocyanate or the histopathology observed.


Author(s):  
Fagbohoun Jean Bedel ◽  
Ekissi Elvis Serge Gbocho ◽  
Yapi Jocelyn Constant ◽  
Kouame Lucien Patrice

Attieke has become one of the most consumed foodstuffs and very popular among the populations of Côte d'Ivoire. It is a steamed cassava semolina with a long transformation process involving different fermentation times. Physicochemical and biochemical analyses have shown that attiéké has many nutritional potentials. Physicochemical parameters (pH, lactic and acetic acids, ethanol, starch, sugars (total and reducing) and vitamin C) were analysed during the process of transforming cassava (Manihot esculenta C.) into attieke. Moisture levels ranged from 61.5% (fresh pulp) to 45% (Attieke). The pH of the fresh cassava (6.1) decreases and reaches 4.7 on the third day of fermentation while that of the attiéké is 5. The acetic (0.48%) and lactic (0.72%) acid contents increase and are respectively 2.40 and 3.60% in the attieke. The ethanol content, which does not exist in the fresh pulp, appears during the fermentation and then decreases from 0.86% (24 hours of fermentation) to 2.40 (attieke). The starch content of cassava pulp decreased from 41.1 (fresh pulp) to 39.5% (fresh Attieke). The vitamin C content of the fresh pulp (69mg/100g) decreases and represents only 1 mg/100g in the fresh attieke.


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