scholarly journals Study of frost resistance of cherry variety Turgenevka during on different rootstocks winter thaws

2020 ◽  
pp. 65-70
Author(s):  
Z. E. Ozherelieva
Keyword(s):  
Frost Resistance ◽  
Abiotic Factors ◽  
Winter Period ◽  
Flower Primordia ◽  
Vegetative Buds ◽  
Subsequent Decrease ◽  
Priority Direction ◽  
Root Stock ◽  
Artificial Freezing ◽  
Thaw Period

Relevance. The study of the resistance of garden cultures to unfavorable abiotic factors of the winter period is a priority direction of scientific studies, because the low winter hardiness can annul advantages the varieties on other signs. Concerning in the middle zone of Russia, studies on the resistance of cherry to frost during the thaw period remain relevant.Methods. The studies were carried out in the laboratory of physiology of fruit plant resistance at VNI-ISPKin2017-2018. The object of research was the Turgenevka cherry variety on new clonal root-stock of the Institute breeding were studied. Control - Turgenevka on the Rubin stock. For artificial freezing in early December the material was prepared for III and IV components of frost hardiness.Results. As a result of artificial freezing, the Turgenevka cherry variety was characterized by frost resistance of vegetative buds and tissues of annual shoots during a three-day winter thaw of + 2°C with a decrease in temperature of -25°C (III component of frost resistance). Inthiscase, thehighest frost resistance of the generative buds of the Turgenevka variety on rootstock 74340, 82987 is shown. After a three-day thaw of +2°C and repeated hardening with a subsequent decrease in temperature to -30°C in March (IV frost resistance component), the Turgenevka variety on the rootstock 74332, 74363, 82987 was characterized by frost resistance of vegetative buds, bark and wood of annualshoots. Moreover, in the studied scion-rootstock combinations, low frost resistance of the generative kidneys was revealed. Only in the studied variety on the rootstock 82987 was the largest percentage of living flower primordia revealed. The conducted studies revealed the Turgenevka variety in a rootstock of 82987 with the greatest potential for frost resistance during the winter thaws.

scholarly journals Evaluation of Finnish apple cultivars (Malus domestica Borkh.)

2020 ◽  
Vol 29 (5) ◽  
Author(s):  
Nina Krasova ◽  
Zoya Ozherelieva ◽  
Anna Galasheva
Keyword(s):  
Frost Resistance ◽  
Abiotic Factors ◽  
Central Zone ◽  
Winter Period ◽  
High Quality ◽  
Malus Domestica Borkh ◽  
Apple Cultivars ◽  
Artificial Freezing ◽  
Continental Climate ◽  
Freezing Chamber

The purpose of this research was to study apple cultivars obtained from Luke (previously MTT Piikkiö, Finland) in order to have good candidates for breeding within the central zone of Russia. The studies were carried out in accordance with the methods of cultivar study and artificial freezing by modeling damaging factors using a freezing chamber. The assessment of the cultivars by yield, commodity and consumer qualities of fruit, resistance to adverse abiotic factors of the winter period is given. The ‘Vuokko’ cultivar was identified with a yield significantly higher than Antonovka. By modeling winter damage factors, ‘Sandra’, ‘Samo’ and ‘Make’ cultivars were identified with the resistance of the cambium and wood at the level of ‘Antonovka’, when the temperature in the of mid-winter dropped to –40 °C after hardening off, with the ability to keep the frost resistance at –25 °C after thaw and ability to restore the hardy state by lowering the temperature to –30 °C after thaw and re-hardening off. Cultivars ‘Sandra’, ‘Samo’ and ‘Make’ are recommended for creating new hardy cultivars with high-quality fruits for the temperate continental climate of Europe.

THE INFLUENCE OF ROOTSTOCK ON THE FROST RESISTANCE OF SWEET CHERRIES DURING THE THAW

2021 ◽  
Vol 66 (1) ◽  
pp. 42-48
Author(s):  
G. Yu. Upadysheva
Keyword(s):  
Vascular Bundle ◽  
Frost Resistance ◽  
Research Center ◽  
Flower Buds ◽  
Climate Chamber ◽  
Laboratory Conditions ◽  
Vegetative Buds ◽  
Sweet Cherries ◽  
Artificial Freezing ◽  
Horticultural Research

Studies on the frost resistance of sweet cherries were carried out in 2019–2020 in the FSBSI of the Federal Horticultural Research Center for Breeding, Agrotechnology and Nursery in laboratory conditions by artificial freezing in the climate chamber TH-6 JEIO TECH-1000. Objects of research: Tyutchevka and Chermashnaya varieties grafted on 8 clonal rootstocks (Colt, Izmailovsky, AVCH-2, V-5-88, VC-13, Moskoviya, VSL-2, Stepnoy Rodnik). The aim of the research is to study the effect of clonal rootstocks on the frost resistance of cherry buds and tissues during the thaw and to identify the most frost-resistant scion-rootstock combinations for the 3rd component. It was found that after freezing at t = -20 °C during the thaw, the safety of flower buds in the Tyutchevka variety in both years of research was more than 80 %, and in the Chermashnaya variety — from 20 to 30 %, depending on the year. The limits of changes in the frost resistance of generative buds of varieties depending on the rootstock are shown: in the Tyutchevka variety — from 66.8 % (V-5-88) to 96.4 % (Izmailovsky), and in the Chermashnaya variety — from 7.7 % (V-5-88) to 65.5 % (AVCH-2). When using the AVCH-2 and Izmailovsky clonal rootstocks, the safety of flower buds increased by 30 % compared to the Colt rootstock. The most frost-resistant scion –rootstock combinations for each variety were identified: Tyutchevka/Izmailovsky and Tyutchevka/Stepnoy Rodnik, which preserved more than 90 % of the living rudiments; Chermashnaya/AVCH-2 (65.5 %) and Chermashnaya/Izmailovsky (40.1 %). Vegetative buds and tissues of annual branches were practically free of freezing in the studied varieties when grafted on the rootstocks of Stepnoy Rodnik and AVCH-2. In the Chermashnaya variety, damage of about 1 point of the phloem and vascular bundle under the buds was noted when grafting on rootstocks V-5-88 and Colt.

STUDY OF PINE STRAWBERRY FROST RESISTANCE BY ARTIFICIAL FREEZING METHOD

1970 ◽  
pp. 26-28
Author(s):  
Z E. Ozherelyeva ◽  
M. I. Zubkova ◽  
D. A. Krivushina
Keyword(s):  
Frost Resistance ◽  
Geographical Origin ◽  
Storage Conditions ◽  
Winter Hardiness ◽  
Winter Period ◽  
Irreversible Damage ◽  
Apical Buds ◽  
Russian Research Institute ◽  
Artificial Freezing ◽  
Main Components

The study of the frost resistance was carried out in 2017–2019 on the basis of the Laboratory of Physiology of Fruit Plant Resistance, Russian Research Institute for Fruit Crop Breeding. Strawberry cultivars of different ecological and geographical origin (‘Kokinskaya Rannia’, ‘Rosinka’, ‘Solovushka’, ‘Tzaritza’, ‘Urozhaynaya TzGL’ – Russia; ‘Sara’ – Sweden; ‘Alba’, ‘Marmolada’ – Italy; ‘Korona’, ‘Sonata’ – Holland) were used as experimental material. The frosts resistance of strawberry varieties was studied in the laboratory conditions was used by artificial freezing. A model of strawberry plant selection was developed to determine the main components of winter hardiness. On the basis of preparation of plant samples for modeling of damaging factors of the winter period, storage conditions of experimental plants were developed. Plant hardening and modeling of damaging factors of the winter period were carried out with the additional modification for strawberries. As a result of the artificial freezing, the damage to the tissues of rhizome and apical buds of shoots of strawberry varieties of different ecological and geographical origin were evaluated in early winter period and during thaws in winter. It was determined that the decrease in the temperature to -15°C at the end of November did not cause irreversible damage to the strawberry varieties. The decrease in the temperature to -20°C in early December increased the damage to the studied strawberry varieties. During the winter it was determined that the strawberry response to the thaw increased by the end of the winter period. The decrease in frost resistance is primarily due to the resumption of growth processes under the influence of positive temperatures. As a result of determining the components of winter hardiness, the varieties Solovushaka, Tzaritza (Russia), Sara (Sweden) and Korona (Holland) showed the greatest potential of frost resistance.

scholarly journals Apple-Tree Resistance to Abiotic Factors in Winter

2013 ◽  
Vol 67 (2) ◽  
pp. 136-144 ◽  
Author(s):  
Nina Krasova ◽  
Anna Galasheva ◽  
Lubov Golishkina
Keyword(s):  
Apple Tree ◽  
Abiotic Factors ◽  
Physiological State ◽  
Winter Period ◽  
Spring Frost ◽  
Tree Resistance ◽  
Winter Conditions ◽  
Scab Immunity ◽  
Apple Cultivars ◽  
Artificial Freezing

Data on the reaction of apple cultivars to unfavorable autumn-winter conditions were analysed and summarised. New scab resistant apple cultivars (gene Vf) obtained from the hybridisation of local adapted varieties with a donor of scab immunity also displayed hardiness to unfavourable winter conditions. As a result of artificial freezing, the cultivars resistant to spring frost (-3.5 oC) during flowering were identified. The mechanism of adaptation to unfavourable winter conditions, and the possibility of preliminary prediction of winter hardiness according to some indicators of the physiological state of tissues in wintering trees were found. In all apple cultivars, increase of the concentrations of sucrose, anthocyanin and cyanidin was noted during the autumn-winter period, but in winter-hardy cultivars this process was more intensive; the amount of cyanidin in the bark of shoots was 6-8 times higher in comparison with non-winter-hardy cultivars. Higher water loss in non-winter-hardy cultivars was observed under conditions of extreme temperatures, which can lead to significant freezing and sunburns late in winter. In winter-hardy cultivars, peroxidase activity decreased in winter, while in the non-winter-hardy cultivars it remains high, due to incomplete hardening of tissues.

scholarly journals Winter resistance and frost resistance of representatives of the genus Cotinus Mill. in the conditions of the Right Bank Forest Steppe of Ukraine

2021 ◽  
pp. 164-175
Author(s):  
Valentina Oksantyuk ◽  
Larysa Koldar
Keyword(s):  
Frost Resistance ◽  
Abiotic Factors ◽  
Winter Hardiness ◽  
Winter Period ◽  
Forest Steppe ◽  
Point Scale ◽  
National Academy Of Sciences ◽  
Crown Shape ◽  
The Right ◽  
Academy Of Sciences

Aim. The goal is to assess the winter and frost resistance of plants of representatives of the genus Cotinus Mill. from the collection of the National Dendrological Park "Sofiyivka" of the National Academy of Sciences of Ukraine and the Botanical Garden named after Academician Alexander Fomin of the Taras Shevchenko National University of Kyiv. Methods. The frost resistance of plants was investigated in the laboratory of plant physiology of the Institute of Horticulture of the National Academy of Sciences of Ukraine, by the method of direct freezing of shoots during the period of forced dormancy of plants. The intensity of damage (browning) of tissues on transverse sections of shoots was assessed using a six-point scale by M. A. Solov'eva (1982). The objects of study were plants C. obovatus Raf., C. coggygria Scop., C. coggygria 'Royal Purple', C. coggygria 'Purpurea'. Winter hardiness was assessed visually on an eight-point scale by S. Ia. Sokolov, (1957). Results. According to estimates of the general freezing of plants in the winter period of 2014–2020, minor injuries were found in all representatives of the genus Cotinus, which were estimated at 1–2 points. The results of experimental studies of plant frost resistance showed that in most variants of the experiment, the tissues of the upper internode and buds froze more. The tissues of the medial part of the shoot turned out to be the most resistant to freezing. According to the results of artificial freeze testing of cut shoots of C. coggygria and C. coggygria 'Royal Purple", sampled in the period of forced dormancy of plants, insignificant tissue damage was revealed at freezing temperature of –25 °С and –30 °С (0.63–1.70), only at temperature of –35 °C, the score of bark damage was 2.3–2.5 points. Conclusions. The low temperatures of the winter period of the study area cannot be considered as limiting abiotic factors that limit the widespread use of the studied representatives of the genus Cotinus in landscape construction. The high potential of frost and winter hardiness of the studied taxa gives grounds to test them in the more northern regions of the Right Bank Forest-Steppe of Ukraine to create garden and park compositions, because due to high decorative flowering and specific crown shape they have an attractive appearance in both group and solitary plantations.

Study of maximum frost resistance of cherry scion/stock combinations

2020 ◽  
Vol 60 (1) ◽  
pp. 62-67
Author(s):  
Z. E. Ozherelieva ◽  
A. S. Lyakhova
Keyword(s):  
Frost Resistance ◽  
Recovery Period ◽  
Winter Hardiness ◽  
Vegetative Buds ◽  
State Study ◽  
Russian Research Institute ◽  
Sweet Cherries ◽  
Hardened State ◽  
Artificial Freezing ◽  
Selection Of

The research was carried out on the basis of the laboratory of stability of physiology of fruit plants at All-Russian research Institute of Breeding of Fruit Crops in 2017-2018. The object of research was the scion/stock combinations of Novella and Turgenevka cherries/82987, C-2-180, 74332, 74340, 74336, 74324 breeding of the Institute. In the study of winter hardiness of cherry, special attention was paid to the ability of varieties to withstand low temperatures in mid-winter. The aim of the research was to study the maximum frost new scion/stock combinations of cherries by artificial freezing and selection of the hardiest of them. The results of the research noted that in the recovery period of organic dormancy in late December, when the cherry plants acquire the maximum hardened state, study scion/stock combinations were characterized by high resistance to frost of bark and wood of annual branches. They noted frost resistance of vegetative buds with reversible damage. Significant differences in the freezing vegetative buds, bark and wood between scion/stock combinations of sweet cherries are shown. Novella and Turgenevka cherry varieties on clonal rootstocks 74332, 74336, characterized by the greatest potential frost resistance of vegetative buds and tissues of annual branches at lower t= -35°C (winter hardiness component II) at the end of December. It is shown that all cherry scion/stock combinations have a low potential frost resistance of generative buds. After exposure to t= -35°C, living rudiments of flower buds preserved only in cherry scion/stock combinations Novella/74332, Turgenevka/74340, 74336 (from 4.0 to 15.4 %). According to the results of artificial freezing scion/stock combination Novella/74332 and Turgenevka / 74332, 74336 were revealed, which were characterized by the maximum frost resistance of generative, vegetative buds, bark and wood.

scholarly journals Biotic vs. abiotic forcing on plankton assemblages varies with season and size class in a large temperate estuary

2020 ◽  
Vol 42 (2) ◽  
pp. 221-237 ◽  
Author(s):  
Gretchen Rollwagen-Bollens ◽  
Stephen Bollens ◽  
Eric Dexter ◽  
Jeffery Cordell
Keyword(s):  
Community Dynamics ◽  
Abiotic Factors ◽  
River Estuary ◽  
Multiple Time Scales ◽  
Nutrient Concentrations ◽  
Winter Period ◽  
Zooplankton Abundance ◽  
Multiple Time ◽  
P Availability ◽  
Lower Estuary

Abstract Large river estuaries experience multiple anthropogenic stressors. Understanding plankton community dynamics in these estuaries provides insights into the patterns of natural variability and effects of human activity. We undertook a 2-year study in the Columbia River Estuary to assess the potential impacts of abiotic and biotic factors on planktonic community structure over multiple time scales. We measured microplankton and zooplankton abundance, biomass and composition monthly, concurrent with measurements of chlorophyll a, nutrient concentrations, temperature and salinity, from a dock in the lower estuary. We then statistically assessed the associations among the abundances of planktonic groups and environmental and biological factors. During the late spring high flow period of both years, the lower estuary was dominated by freshwater and low salinity-adapted planktonic taxa, and zooplankton grazers were more strongly associated with the autotroph-dominated microplankton assemblage than abiotic factors. During the early winter period of higher salinity and lower flow, nutrient (P) availability exerted a strong influence on microplankton taxa, while only temperature and upwelling strength were associated with the zooplankton assemblage. Our results indicate that the relative influence of biotic (grazers) and abiotic (salinity, flow, nutrients and upwelling) factors varies seasonally and inter-annually, and among different size classes in the estuarine food web.

Seasonal studies of vegetative buds of Helianthus tuberosus: concentration of nuclei in phase G1 during winter dormancy

1981 ◽  
Vol 59 (10) ◽  
pp. 1918-1927 ◽  
Author(s):  
S. S. Tepfer ◽  
Arlette Nougarède ◽  
Pierre Rondet
Keyword(s):  
Mitotic Activity ◽  
Mitotic Index ◽  
Developmental Stages ◽  
Central Zone ◽  
Winter Period ◽  
Helianthus Tuberosus ◽  
Winter Dormancy ◽  
Feulgen Staining ◽  
Vegetative Buds ◽  
Terminal Buds

The following vegetative buds were studied at several developmental stages during the course of the year: from November through February dormant terminal buds of subterranean tubers; in March, newly reactivated buds of young shoots; in June, terminal buds of horizontal underground stolons that will form tubers; and in July, the terminal buds of erect aerial shoots. Microdensitometric studies of DNA levels after Feulgen staining showed that during the winter period of dormancy, from November through February, the temporary arrest of growth and morphogenesis is accompanied by a concentration of nuclei in phase G1 (2C level) of a diploid cycle for all nuclei in the terminal meristems of the tubers. In March, reactivation occurs uniformly throughout the meristem without any zonal differences. The G1 phase remains predominant in the cycle and mitotic activity increases uniformly. In the meristems of young underground stolons, beginning in the month of June, signs of concentration at the 2C level again are perceptible. The nuclei of the apical meristems of erect shoots are also diploid at 2C and 4C. In the very large nuclei in the central zone of the tunica where the mitotic index is very low, the distribution of DNA levels shows that nuclei are present at all phases of the cycle. These results are discussed and compared with other species in temporary dormancy and in regard to the concept of nonpolysomatic species.

RELATIVE AND ABSOLUTE ELECTROLYTIC CONDUCTANCE TESTS FOR FROST HARDINESS OF APPLE VARIETIES

1960 ◽  
Vol 40 (4) ◽  
pp. 630-637 ◽  
Author(s):  
J. Wilner
Keyword(s):  
Frost Resistance ◽  
Laboratory Method ◽  
Frost Hardiness ◽  
Apple Trees ◽  
Electrolytic Conductance ◽  
Laboratory Methods ◽  
Total Conductance ◽  
Average Survival ◽  
Artificial Freezing ◽  
Frost Injuries

A laboratory method for testing frost hardiness of apple trees is described. Results demonstrated that diffused electrolytes from pre-hardened twigs provided a reliable means of testing their hardiness. There was a definite association between increased diffusion of electrolytes from hardened tissues and increased injury of twigs. This association was found useful in establishing both the relative degrees of hardiness of three named varieties of apples, Antonovka, McIntosh and Northern Spy, and the absolute values for the ultimate frost resistance of their tissues. Thus, although the total conductance of electrolytes of the three varieties was about the same before the artificial freezing treatments, ranging from 900 to 1000 micromhos, the average survival and the conductance of diffused electrolytes were as follows: hardy Antonovka 71 per cent and 296 micromhos; less hardy McIntosh 53 per cent and 445 micromhos; and least hardy Northern Spy 23 per cent and 611 micromhos. Results also established quantitative values for the ultimate frost hardiness of apple trees, namely: no appreciable injury when the conductance from hardened tissues of twigs was within 200 to 250 micromhos or less. Conductance of electrolytes above 350 to 450 micromhos generally signified total killing; whereas intermediary readings indicated partial injury to twigs.The method used also permitted a study of the effects of several factors considered of vital importance in: (a) developing frost resistance of overwintering plants such as seasonal maturity, cold hardening and dehardening of tissues, and (b) causing excessive frost injuries such as increasing intensities and rates of freezing temperatures. The validity of the used laboratory methods is discussed.

scholarly journals Frost hardiness of introduced sea buckthorn (Hippophae rhamnoides L.) genotypes in Central Russia

2016 ◽  
Vol 70 (2) ◽  
pp. 88-95 ◽  
Author(s):  
Zoya Ozherelieva ◽  
Pavel Prudnikov ◽  
Natalia Bogomolova
Keyword(s):  
Fractional Composition ◽  
Amylase Activity ◽  
Sea Buckthorn ◽  
Hippophae Rhamnoides ◽  
Frost Hardiness ◽  
Winter Period ◽  
Hippophaë Rhamnoides ◽  
Central Russia ◽  
Artificial Freezing ◽  
Hippophae Rhamnoïdes L

AbstractSea buckthorn (Hippophae rhamnoides L.) genotypes of different ecological and geographical origins were tested. Frost hardiness extent was determined during winter by artificial freezing, which allowed identify varietal differences in frost hardiness of buds and tissues of sea buckthorn shoots. In order to identify differences in adaptation to low temperature we determined the fractional composition of water in annual shoots; and proline and malone dialdehyde concentration and total amylase activity in bark of shoots of female and male genotypes. In December, the sea buckthorn genotypes were in deep dormancy, when maximum frost hardiness of buds and tissues of annual shoots occurred. During the next months, the frost hardiness of buds and shoot tissues decreased during the state of exogenous dormancy. The performed experiment gave an opportunity to select hardy genotypes ‘Kenigsbergskaya’, ‘Surpriz Baltiki’, B 23-34, and ‘Prima Dona’ for use in further breeding, as they had demonstrated stable hardiness during the whole winter period according to all of the components of winter hardiness.

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