scholarly journals PENGARUH PEMBERIAN SAGU DIBANDING NASI TERHADAP BERAT BADAN TIKUS WISTAR

e-CliniC ◽  
2013 ◽  
Vol 3 (2) ◽  
Author(s):  
Eny A. Watumlawar ◽  
Sarah M. Warouw ◽  
Stefanus Gunawan

Abstract: Weight is one measure that gives description of the tissue mass including body fluids. Weight change is nfluenced by nutriment consumption. Sago contains high carbohydrates that can increase body weight. This study aimed to obtain the effect of sago compared to rice to body weight of wistar rats. This was a random laboratory experimental pre-post test with control group design. Subjects were male wistar rats, aged 5-6 months. The rats were divided in two groups: rice group as control and sago group. Sago was cooked in papeda form as much as 75 g of dried sago and 300 mL water. The rats were fed for 2 weeks. Data were analyzed by using the Wilcoxon test. The results showed that the sago group showed an increase of body weight significantly (p=0,001) meanwhile the rice group lose body weight significantly (p=0,001). Conclusion: Sago can increase body weight of wistar rats significantly.Keywords: body weight, sago, riceAbstrak: Berat badan merupakan salah satu ukuran yang memberikan gambaran massa jaringan termasuk cairan tubuh. Salah satu yang memengaruhi berat badan yaitu dengan mengonsumsi makanan bergizi. Sagu memiliki kandungan karbohidrat (pati) yang besar dan dapat meningkatkan berat badan. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian sagu terhadap berat badan dibanding nasi pada tikus wistar. Desain penelitian ini ialah eksperimental laboratorium acak pre-post test with control group design. Subjek penelitian ialah tikus wistar jantan berusia 5-6 bulan dibagi atas 2 kelompok: kelompok nasi (kontrol) dan kelompok sagu. Sagu dimasak dalam bentuk papeda sebanyak 75 g sagu kering dan 300 mL air. Tikus diperlihara selama 2 minggu. Berat badan diukur dengan menggunakan timbangan. Data dianalisis dengan uji Wilcoxon. Hasil penelitian menunjukkan bahwa pemberian sagu sebanyak 2 cc setiap hari selama seminggu mampu meningkatkan berat badan secara bermakna (p = 0,001) sedangkan pemberian nasi menurunkan berat badan (p=0,001). Simpulan: Pemberian sagu dapat meningkatkan berat badan tikus wistar secara bermakna.Kata kunci: berat badan, sagu, nasi

2021 ◽  
Vol 1 (2) ◽  
pp. 45-49
Author(s):  
Syahran Wael ◽  
Didik Wahyudi ◽  
Tayeb Wael ◽  
Zaid Mohamed Jaber

Nigella sativa oil is antioxidant compound has the effect that serves to prevent cellular damage. The effect of Nigella sativa oil in the motility and total count of spermatozoa wistar rats. Experimental research with the design of post test only control group design. Wistar rats consists of 24 head and divided into 4 groups consist of control and treatment group. The control group was distilled. The first treatment was of Nigella sativa oil everydays as much with dose 150 mg/kgbw, 250 mg/kgbw, and 350 mg/kgbw, for 16 days, . Statistic test for motility and count of sperm wistar rats use the Kruskal-Wallis followed by Mann Whitney test. Avarage value of motility in the control (21.67±9.832) its lower than treatment. In dose 350 mg/kgbw its highest (52.33±13.292) compare in the treatment 250 mg/kgbw (40.67±17.512) and 150 mg/kgbw (30.67±8.165). avarage value of count sperm in the control (130.83±41.877) its lower than treatments. In dose 350 mg/kgbw its highest (199.67±23.480) compare in the treatment 250 mg/kgbw (187.50±74.538) and 150 mg/kgbw (140.83±32.568). Administration of Nigella sativa oil occur to enhancement motility and number of spermatozoa wistar rats.


2020 ◽  
Vol 1 (1) ◽  
pp. 5-9
Author(s):  
Vianney Natasaputra ◽  
Taufik Eko Nugroho

Background :  Pain is one of the most common complaint in patients. Combination of paracetamol and codeine is an alternative analgesic combination in chronic pain management. They belong to different group of analgesic and have different mechanism of action. Combination of these drugs give a better potential in pain management.  However, these drugs also have potential side effect on the kidney.Methods : An experimental study of post-test only control group design. The sample were 20 Wistar rats, randomized into 4 groups: control group, a group receiving paracetamol 32 mg/kg body weight, a group receiving codeine 1,9 mg/kg body weight, and a group receiving paracetamol 32 mg/kg body weight and codeine 1,9 mg combination, administered 4 times a day orally using gastric instillation for 28 days. At the 29th day, blood is collected from retro orbital vessel to measure the serum creatinine levels. Statistical analysis was conducted using ANOVA TestResult : Obtained from statistical analysis there is no significant difference in serum creatinine levels in Wistar rats given all treatment group (p > 0,05).Conclusion : There is no significant difference in serum creatinine levels between administration of paracetamol and codeine combination compared to the control group.Keywords : Paracetamol, Codeine, Creatinine Serum, Pain


DENTA ◽  
2019 ◽  
Vol 12 (2) ◽  
pp. 8
Author(s):  
Isidora Karsini ◽  
Farhana Nur Fadhila ◽  
Nafiah Nafiah

<p><strong><em>Background:</em></strong><em> </em><em>Channa striata contains important compounds in the process of tissue synthesis and important role in wound healing, such as albumin, zinc (Zn), copper (Cu), iron (Fe) and unsaturated fat acids. <strong>Purpose :</strong> To determine the effect of Channa striata extract to the amount of neutrophil in healing process of traumatic ulcer. <strong>Material and Method :</strong> The design of this study used randomized post test only control group design. 18 wistar rats were divided into 6 groups, i.e: K-1 (no treatment for 1 day), K-3 (no treatment for 3 days), K+1 (giving hyaluronic acid 0.2% for 1 day), K+3 (giving hyaluronic acis 0.2% for 3 days), P1 (giving Channa striata extract 100% for 1 day), P3 (giving Channa striata extract 100% for 3 days). <strong>Results :</strong> There was a decrease in the number of neutrophil in the treatment group. The mean and standart deviation of the number of neutrophil in the K-1 group (75,96±3,65), K-3 (50,76±2,04), K+1(72,96±2,97),K+3(45,06±2,83),P1(54,20±1,31),P3(32,50±,85). <strong>Conclusion :</strong> The using of Channa striata extract 100% for 3 days are the most effective in decreasing the amount of neutrophil in healing process of traumatic ulcer of Rattus Novergicus Strain Wistar.</em></p>


2021 ◽  
Vol 8 (2) ◽  
pp. 156
Author(s):  
Endah Ariyati Eko Ningtyas ◽  
Oedijani Santoso ◽  
Udadi Sadhana ◽  
Siti Sunarintyas

ABSTRACTBackground: Inflammatory and/or non-inflammatory processes play a role in stimulating pulp repair and the formation of hard tissue, namely reparative dentin. Macrophages play a role in the pathogenesis and chronic inflammatory disorders. The combination casein lactoferrin of bovine colostrum as an immunomodulator has therapeutic potential. This study aims to determine the therapeutic effect and duration of application of the combination of casein lactoferrin of bovine colostrum, on the expression of macrophages as pulp capping.Method: This study was a true experimental laboratories post test only control group design, consisting of three groups of 60 male wistar rats with 4 observation times, namely day to day 7, 14, 21 and 28 each of 5 mice. The maxillary 1st molars were prepared until the roof of the pulp was exposed. Three groups, namely the combination of casein and lactoferrin bovine colostrum (CKL) and calcium hydroxide (K1) and the untreated group (K0). Each group was filled with glassionomer as a permanent restoration. The tissue was made histological preparations with hematoxylin-eosin staining and the number of macrophages were counted, then analyzed by two way ANOVA and post hoc LSD tests.Result: The results showed that the therapeutic effect and duration of application of the combination of casein and lactoferrin bovine colostrum on the expression of macrophages as pulp cappingConclusion: The combination of casein and lactoferrin of bovine colostrum as capping material can increase the number of macrophages in the healing process of dental pulp.


Author(s):  
Putu Wira Kusuma Putra ◽  
Andriani Astuti Kusuma Raharjo ◽  
AA Kompiang Ngurah Darmawan

Keluarga pasien mempunyai peranan peting dalam pencegahan infeksi nosokomial dengan cara meningkatkan perilaku mencuci tangan dengan baik. Akan tetapi pelaksanaan cuci tangan pada keluarga pasien belum berjalan secara optimal karena disebabkan banyak keluarga pasien tidak mengetahui cara mencuci tangan yang benar. Tujuan penelitian ini adalah untuk mengetahui pengerauh pendidikan kesehatan dengan media leaflet terhadap perilaku Cuci Tangan Pengunjung di Rumah Sakit Umum Bali Royal. Penelitian ini menggunakan rancangan quasy experiment dengan pendekatan pre test and post test control group design. Pengambilan sampel dengan teknik quata sampling yaitu sebesar 29 orang yang terdiri dari perlakuan yang mendapatkan pendidikan kesehatan melalui media leaflet dan kelompok kontrol yang mendapat prosedur edukasi yang berlaku di ruangan. Data dikumpulkan dengan mengunakan kuesioner dan dianalisis menggunakan uji wilcoxon test, dan Mann whitney U test. Hasil uji statistik Mann Whitney U test menunjukkan ada perbedaan perilaku pengunjung mencuci tangan antara kelompok perlakuan dan kontrol dengan p=<0,0001. Pendidikan kesehatan mengenai cuci tangan melalui media leaflet efektif meningkatkan perilaku pengunjung dalam mencuci tangan.  Perawat diharapkan dapat memberikan penjelasan mengenai pentingnya mencuci tangan kepada keluarga pasien sehingga infeksi nosokomial dapat dicegah.


2018 ◽  
pp. 134-141
Author(s):  
Luhur Arifian ◽  
Joko Kismanto

ABSTRAK Pada penyakit asma, serangan umumnya datang pada malam hari, tetapi dalam keadaan berat serangan dapat terjadi setiap saat tidak tergantung waktu. Inspirasi pendek dan dangkal, mengakibatkan penderita menjadi sianosis, wajahnya pucat dan lemas, serta kulit banyak mengeluarkan keringat. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian posisi Semi fowler terhadap respiration rate pada pasien asma bronkial di Puskesmas Air Upas Ketapang   Jenis penelitian ini adalah penelitian kuantitatif dengan rancangan Quasi Eksperimental dengan Pre and post test with control group design. Populasi dalam penelitian ini adalah sejumlah 48 orang yang mengalami asma bronkial di Puskesmas Air Upas Kabupaten Ketapang, Kalimantan Barat. Pemilihan sampel dilakukan dengan metode purposive sampling dengan jumlah sampel pada penelitian ini adalah 42 responden. Analisa data dalam penelitian ini menggunakan uji Wilcoxon.   Hasil penelitian menunjukkan uji Wilcoxon dengan nilai p value 0,000 sehingga ada pengaruh pemberian posisi semi fowler terhadap respiration rate pada pasien asma bronkial di Puskesmas Air Upas Ketapang   Kata Kunci: posisi semi fowler, respiration rate, asma bronkial     ABSTRACT In asthma, the attacks usually come at night, but in a state of severe attacks can occur at any time does not depend on time. Inspiration short and shallow, resulting in the patient became cyanotic, his face pale and limp, and skin a lot of sweat. This study aimed to determine the effect of semi fowler position against respiration rate in patients with bronchial asthma in the Main Clinic Air Upas Ketapang. This research used the quasi experimental quantitative method with the pre and post test with control group design. It’s population was 48 asthma sufferers at the main clinic Air Upas Ketapang of west Borneo. The samples of research were determined through the purposive sampling technique and consisted of 42 respondents who were divided into two groups: 21 in the control group and 21 in the experimental group. The data of research were analyzed by using the Wilcoxon’s analysis.The results showed the Wilcoxon test with p value of 0.000 so that there is the effect of semi fowler position against respiration rate in patients with bronchial asthma in the Main Clinic Air Upas Ketapang.   Keywords: position semi fowler, respiration rate, bronchial asthma  


2021 ◽  
Vol 21 (no 1) ◽  
Author(s):  
Gede Widhiantara ◽  
Anak Agung Ayu Putri Permatasari ◽  
Putu Angga Wiradana

This study aims to determine the morphology of Leydig cells in hyperlipidemic wistar rats after having administered with sembung (Blumea balsamifera) extract orally. This study utilised a randomized post-test only control group design. The sample in this study were 16 adult male wistar rats (Rattus novergicus) aged 3-4 months with a body weight of 150-200 grams, which were equally and randomly divided into two groups, namely control group (hyperlipidaemia and sterile aquadest) and treatment group (hyperlipidaemia and sembung leaf extract). Hyperlipidaemia was induced with lard administration for 50 days. Data were analysed statistically using the Independent t-test. The results showed that the number of Leydig cells in the treatment group was higher than the control group with 68.13 ± 1.89 and 55.63 ± 1.92 cells respectively (P <0.05). In addition, the mean Leydig cell core diameter of the control group, 5.00 ± 0.34 µm, was smaller compared to the treatment group which was 5.80 ± 0.20 µm (P <0.05). It can be inferred that sembung leaf extract provides a protective effect against damage to Leydig cells due to hyperlipidaemia.


2019 ◽  
Vol 10 (2) ◽  
pp. 95-102
Author(s):  
Sudarman Sudarman

Penelitian ini bertujuan untuk mengetahui pengaruh pelatihan bantuan hidup dasar terhadap peningkatan keterampilan siswa kelas XI Sekolah Menengah Kejuruan Baznas Sulsel. Penelitian ini menggunakan pendekatan kuantitatif dengan jenis penelitian quasi eksperimen dan desain penelitian pre and post test control group design. Pada desain ini peneliti  melakukan intervensi pada dua kelompok. Kelompok pertama sebagai kelompok perlakuan dan kelompok kedua sebagai kelompok kontrol. Populasi dalam penelitian ini adalah seluruh siswa-siswi Sekolah Menengah Kejuruan Baznas Sulsel kelas XI yang berjumlah 42 orang yang terdiri dari 21 orang kelompok intervensi dan 21 kelompok kontrol. Uji yang digunakan Wilcoxon Test adalah untuk mengetahui perbedaan keterampilan sebelum dan sesudah pada kedua kelompok. Hasil penelitian menunjukkan ada pengaruh pelatihan bantuan hidup dasar terhadap keterampilan Siswa Kelas XI di Sekolah Menengah Kejuruan Baznas Sulawesi Selatan dengan nilai α=0,001 (<0,05). Ada pengaruh pemberian panduan tentang bantuan hidup dasar terhadap keterampilan Siswa Kelas XI di Sekolah Menengah Kejuruan Baznas Sulawesi Selatan dengan nilai α= 0,008 (<0,05). Ada perbedaan keterampilan antara siswa yang diberikan pelatihan dan yang tidak diberikan bantuan hidup dasar Siswa Kelas XI di Sekolah Menengah Kejuruan Baznas Sulawesi Selatan dengan nilai α=0,015 (<0,05). Kesimpulan dalam penelitian ini adalah ada perbedaan keterampilan antara siswa yang diberikan pelatihan dan yang tidak diberikan bantuan hidup dasar Siswa Kelas XI di Sekolah Menengah Kejuruan Baznas Sulawesi Selatan. Diharapkan pihak pengelola Sekolah Menengah Kejuruan Baznas Sulawesi Selatan perlu melakukan perencanaan pelatihan bantuan hidup dasar secara ruti, menyediakan alat/pantom resusitasi jantung paru, panduan maupun video prosedural basic life support untuk meningkatkan pengetahuan dan keterampilan siswa dan perawat komunitas kota Makassar perlu melakukan program pelatihan bantuan hidup dasar untuk sekolah menengah kejuruan.


2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Risnawati A. Haris ◽  
Lydia Tendean ◽  
Grace Turalaki

Abstract: The coffee beans composed of the compound caffeine, palmitic acid, linoleic acid and acid stearic. The content of caffeine in coffee can improve spermatozoa motility that are not motile by inhibiting cyclic nucleotide phosphodiesterase and affects the intracellular levels of cyclic AMP. Spermatozoa quality can be affected by various factors such as stress. In nocturnal animals, dark periode becomes a signal to do activity by suprachiasmatic nucleus. The change in activity of the nocturnal animals from initial condition in the dark then exposed to light can leads to stress. This study was aimed to determine the effect of coffee on the spermatozoa quality of wistar rats which had been exposed to stress. This was an experimental analytical study with a post-test only control group design. The study was conducted for 50 days from September to November 2016. The sample consisted of 9 rats divided into three groups: a control group (P0) is given only exposure to stress in the form of light, the treatment group 1 (P1) is given exposure to stress in the light form and liquid coffee 1 ml / kg and the treatment group 2 (P2) is given exposure to stress in the light form and coffee extracts. The results showed that there were significant differences in the concentration and motility treatment group 1 (P1) to the control group (P0) which is p <0.05 and significant differences in sperm motility treatment group 2 (P2) to the control group (P0) which is p <0.05. Conclusion: Coffee administration could improve the quality of spermatozoa of wistar rats exposed to stress.Keywords: stress, coffee, quality of spermatozoa. Abstrak: Biji kopi tersusun dari senyawa kafein, asam palmatik, asam linoleat dan asam stearik. Kandungan kafein yang terdapat didalam kopi mampu meningkatkan motilitas spermatozoa yang tidak motil dengan cara menghambat siklus nukleotida fosfodiesterase dan mempengaruhi level intraseluler dari siklus AMP. Kualitas spermatozoa juga dapat dipengaruhi oleh berbagai faktor lain seperti stres. Pada hewan nokturnal periode gelap menjadi sinyal untuk beraktiftas dilakukan oleh suprachiasmatic nucleus. Perubahan aktivitas pada hewan nokturnal dari kondisi awalnya ditempat yang gelap lalu diberikan paparan cahaya dapat menyebabkan terjadinya stres. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian kopi terhadap kualitas spermatozoa tikus wistar yang terpapar stres. Jenis penelitian ialah eksperimental analitik dengan post-test only control group design. Penelitian dilakukan selama 50 hari dari bulan September – November 2016. Sampel sebanyak 9 ekor tikus dibagi menjadi tiga kelompok yaitu : kelompok kontrol (P0) hanya diberikan paparan stres berupa cahaya, kelompok perlakuan 1 (P1) diberikan paparan stres berupa cahaya dan cairan kopi 1 mL/KgBb dan kelompok perlakuan 2 (P2) diberikan paparan stres berupa cahaya dan ekstrak kopi. Hasil penelitian menunjukkan adanya perbedaan yang signifikan pada konsentrasi dan motilitas spermatozoa kelompok perlakuan 1 (P1) terhadap kelompok kontrol (P0) yaitu p<0,05 dan perbedaan yang signifikan pada motilitas spermatozoa kelompok perlakuan 2 (P2) terhadap kelompok kontrol (P0) yaitu p<0,05. Simpulan: Pemberian kopi meningkatkan kualitas spermatozoa yang terpapar stres. Kata kunci: stres, kopi, kualitas spermatozoa


2018 ◽  
Vol 2 (1) ◽  
Author(s):  
Widya Addiarto ◽  
Shinta Wahyusari

 AbstrakSaat ini skill perawat ketika menjadi volunteer pada saat terjadi bencana masih rendah. Upaya untuk meningkatkan skill tersebut salah satunya adalah dengan cara memberikan pendidikan bencana sejak awal kepada mahasiswa keperawatan yang salah satunya dapat mengguanakan media Tabletop Disaster Exercise (TDE). Penilitian ini bertujuan untuk mempelajari efektivitas media Tabletop Disaster Exercise dalam meningkatkan skill triage dan alur rujukan korban bencana. Desain penelitian yang digunakan adalah quasy experiment dengan menggunakan pre-post test with control group design. Jumlah populasi adalah 188 mahasiswa. Sampel diambil menggunakan purpossive sampling dan didapatkan jumlah responden sebesar 36 dibagi menjadi 2 kelompok yaitu kelompok kontrol dan perlakuan. Data dianalisis menggunakan uji Wilcoxon dan didapatkan ada perbedaan triage (ρ = 0,001) dan alur rujukan (ρ = 0,000) sedangkan pada kelompok kontrol hasil didapatkan perbedaan skill triage (ρ = 0,001) dan alur rujukan (ρ = 0,001). Hasil uji Mann Whitney yaitu terdapat perbedaan skill triage dan alur rujukan dari responden antara kelompok perlakuan dengan kelompok kontrol dengan nilai signifikansi masing-masing 0,013 dan 0,004. Dapat disimpulkan bahwa pada kelompok perlakuan dan kontrol sama-sama dapat meningkatkan skill triage dan alur rujukan dari masing-masing responden, akan tetapi dari analisis kedua metode didapatkan hasil yang signifikan yang menunjukkan adanya perbedaan antara media TDE dengan metode konvensional. Kata kunci : tabletop disaster exercise, triage, alur rujukan  AbstractToday, the nurses' skills when volunteering at disaster management are still low. therefore to improve these skills is by providing disaster education from the beginning to nursing students. One of the learning media that is expected to be able to answer  these problems is the Tabletop Disaster Exercise (TDE). This study aims to determine the effectivity  of Tabletop Disaster Exercise in improving triage and referral flow of disaster victims. The research design used quasy experimental with two groups pre-post test design. 36 subjects in total (2 groups of 18 members) was selected using purpossive sampling. Data were analyzed using Wilcoxon test and Mann whittney test. The results showed there were differences in triage (ρ = 0,001) and referral flow (ρ = 0,000) while in the results of the control group, there were differences in triage (ρ = 0.001) and referral flow (ρ = 0.001). The result of the Mann Whitney test showed there were differences in respondents’s skill between the treatment group and the control group with a significance value of triage (ρ = 0.013) and referral flow (ρ = 0.004). Thus it can be concluded that both the treatment and control groups can equally improve the triage and referral Flow skills of each respondent, but from the analysis of both methods, significant results are showed by the differences between TDE and conventional methods.Keywords: tabletop disaster exercise, triage, referral flow


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