scholarly journals Melatonin menurunkan berat badan tetapi tidak menurunkan kadar TNF-α pada tikus wistar jantan yang diberi minyak jelantah selama 28 hari

2017 ◽  
Vol 5 (2) ◽  
pp. 127-132
Author(s):  
Anugrah Novianti ◽  
Edi Dharmana ◽  
Nyoman Suci Widyastiti
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Treated Group ◽  
Waste Cooking Oil ◽  
The Body ◽  
Control Group ◽  
Cooking Oil ◽  
Tnf Α ◽  
Significant Difference ◽  
Male Wistar Rats

Backgound: Non Alcoholic Fatty Liver Disease (NAFLD) occurs when the intake and free fatty acid synthesis occurs more frequently than its oxidation and resecretion in the blood. Melatonin is a powerful antioxidant that can boost the synthesis of endogenous antioxidants in the body, suppress the inflammatory response and inhibit the formation of steatosis.Objective: To analyze the effect of melatonin supplementation in reducing body weight andTNF-α levels in male Wistar rats were fed by waste cooking oil.Methods: True experimental studyusing post-test only control group design. This study was done on 18 male wistar rats were divided into 3 groups : the positive control group (P0) was administrated waste cooking oil, the treated group 1 (P1) was administrated waste cooking oil and 5mg/kgBW melatonin, and the treated group 2 (P2) was administrated waste cooking oil and 10mg/kgBW melatonin for 28 days.Data analysis using One Way ANOVA test and followed by Tukey test to determine the most effective dose of melatonin.Results: There was significant difference in body weightbetween P2group and K0 group (p=0,019) with the mean body weight difference was 19,167g lower than K0 group. There was no difference in TNF-α levels between the three groups (p=0,155). Conclusion: Melatonin dose of 10mg/kgBWloses body weight male Wistar rats have given by waste cooking oil for 28 days, but does not reduce TNF-α levels.

scholarly journals Effects of proportional high-protein/low-carbohydrate formulated diet consumption in diabetic rats: Beneficial impact on glycemic and weight control

2020 ◽  
Vol 20 (07) ◽  
pp. 16984-16996
Author(s):  
MMC Anyakudo ◽  
◽  
DO Adeniji ◽  
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Treated Group ◽  
Morphological Features ◽  
High Protein ◽  
Control Group ◽  
Formulated Diet ◽  
Low Carbohydrate ◽  
Male Wistar Rats ◽  
And Control

The metabolic response to nutrient ingestion and the rate of digestion and absorption of nutrient molecules in bowel physiology plays an important role in the metabolic control of some human chronic non-infectious diseases. This experimentally-controlled designed nutritional study which lasted eight weeks aimed to determine the effects of proportional high-protein/low-carbohydrate (HP/LC) formulated diet on glycemic tolerance, glycemic control, body weight, organ weight and organ morphometry in healthy and diabetic adult male Wistar rats. Twenty-four male Wistar rats purchased from a disease-free stock were randomly categorized into four groups (n = 6, each) after two weeks acclimatization period in raised stainless steel cages with 6 mm2mesh floor and replaceable numbered blotters papers placed under each cage in a well-ventilated animal house. Animal groups include: Healthy control group (HC), Healthy treated group (HT), Diabetic control group (DC) and Diabetic treated group (DT. The animals were fed according to the experimental design with water ad libitumfor eight weeks. Diabetes was inducted with freshly prepared alloxan monohydrate solution (150 mg/kg bw, intraperitoneally). Body weights and fasting blood sugar concentrations were measured twice weekly, while oral glucose tolerance test was conducted on the last day of the eighth-week study and subsequently followed by organs extraction after anesthesia for weight and gross assessment. Proportional high-protein/low-carbohydrate formulated diet caused significant reduction in mean body weight of treated diabetic (DT: 22.6%; P= .001) and healthy (HT: 5.8%; P= .007) rats while the control animals on control diet recorded significant (P< .05) increase in body weight gain (DC: 12.4%; HC: 11.2%). Glycemic tolerance and control improved significantly in diabetic treated rats over that of the healthy treated rats. Gross morphometry of the extracted organs (kidneys, liver, heart, lungs, spleen and testes) revealed sustained normal morphological features without any visible lesion. In conclusion, consumption of proportional high-protein/low-carbohydrate formulated diet enhanced body weight reduction and sustained normal organ morphological features with good glycemic tolerance and control in experimental rats, suggesting its dietary potentiality, safety and suitability to ameliorate obesity-related diabetes.

scholarly journals Effects of Oral Administration of Monosodium Glutamate (MSG) on Obesity in Male Wistar Rats (Rattus Norvegicus)

2021 ◽  
Vol 5 (9) ◽  
pp. 879-882
Author(s):  
Mulyati Sri Rahayu ◽  
Sri Wahyuni ◽  
Yuziani
Keyword(s):  
Body Weight ◽  
Adult Male ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Body Weight Gain ◽  
Monosodium Glutamate ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Group 2

Introduction: Monosodium glutamate (MSG) is one of the most widely employed food enhancers. Although the umami compound, controversy persists regarding the effects of MSG intake on body weight. Chronic MSG intake may result in excessive body weight gain and obesity. Consumption of MSG result in organ damage, cardiovascular disease, oxidative stress, and also risk factors for obesity. This study aims to determine the effect of oral MSG on obesity in adult male Wistar rats (Rattus norvegicus).Methods: This true experimental study used the post-test control group design. Twenty-four adult male Wistar rats were randomly divided into four groups: control (received distilled water), Group 1 (MSG 0.378 mg/gr BW), Group 2 (0.756 mg/gr BW) and Group 3 (1.512 mg/gr BW). The obesity parameter was obtained by the Lee index. Kruskal-Wallis test follows by Mann-Whitney test were used to compare the Lee index between groups.Results: Lee’s index mean for each group was 358.4%, 314.1%, 287.8%, and 320.9%, respectively. The Kruskal Wallis test showed a significant difference in the Lee index between groups (p = 0.043). A follow-up test using Mann-Whitney found a significant difference between group 2 and the control group (p = 0.043, p <0.05). The mean of Lee index of group 2 was 70.51% lower than the control group.Conclusion: This study concluded that Lee index was not increased in MSG-treated rats than in the control group after oral MSG intervention for 21 days.

scholarly journals Utilization of Snakehead Fish Meat for Increasing Kidney Function in Rat Experiencing Physiological Stress

2018 ◽  
Vol 10 (3) ◽  
pp. 553-558
Author(s):  
Sunarno Sunarno ◽  
Hajar Sokmawati ◽  
Rully Rahadian
Keyword(s):  
Body Weight ◽  
Kidney Function ◽  
Wistar Rats ◽  
Physiological Stress ◽  
The Body ◽  
Control Group ◽  
Kidney Weight ◽  
Significance Level ◽  
Fish Meat ◽  
Male Wistar Rats

Physiological stress for a long time can lead to impaired kidney organ function. Snakehead fish meat are known to contain important nutrients such as albumin, glutamine, glycine, cysteine, and various minerals that can improve kidney function. This study was conducted to examine and analyze the effect of snakehead fish supplementation in feed towards body weight, kidney weight, diameter and number of glomerulus in Wistar rats with physiological stress. This study used 20 male Wistar rats divided into 5 groups consisted of 1 control group (P0) and 4 treatment groups (P1: 5%, P2: 10%, P3: 15%, P4: 20%). The treatments were performed with 4 times repetition. This study used Completely Randomized Design (CRD). The variables measured were body weight, kidney weight, diameter and number of renal glomerulus. The data obtained were analyzed using Analysis of Variance (ANOVA) at 5% significance level and continued with Duncan test with 5% significance level. The results of this study were: the supplementation of snakehead fish with 20% of concentration can increase the body weight; concentration 10; 15; and 20% can increase the weight of the kidney; concentrations of 5%, 10%, 15%, and 20% can increase the glomerular diameter in Wistar rats with physiological stress. The supplementation of snakehead fish with concentration of 5% to 20% did not affect the number of glomerulus of Wistar rats with physiological stress. Results of this study is useful as information for people who study the mechanism of kidney repair due to physiological stress.

scholarly journals A Comparative study on Efficacy of Lutein and Atorvastatin on Lipid Profile and Lipoprotein(A) in Hypercholesterolemic male Wistar Rats

2021 ◽  
Vol 14 (01) ◽  
pp. 503-511
Author(s):  
Soundarya Priyadharsini K ◽  
Mali Kalpana Ramanna ◽  
Somu L ◽  
Krishna Prasad T
Keyword(s):  
Body Weight ◽  
Lipid Profile ◽  
Wistar Rats ◽  
Treated Group ◽  
Diet Group ◽  
Lipoprotein A ◽  
Group I ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Progression Of Atherosclerosis

Background Hypercholesterolemia is the predominantfactor in developing atherosclerosis and myocardial diseases.A major contributor for the progression of atherosclerosis is abnormalities in lipid and lipoprotein metabolism.Hence the objectives of the study was to estimatethe comparative efficacy of Lutein with atorvastatin on lipid profile and lipoprotein(a) and to estimate the histopahthological changes in hypercholesterolemic male wistar rats. Materials and Methods Experimental Wistar rats (male) were grouped into six. Each group contains 6 rats. Group I is control. Group II received cholesterol rich diet. Group III received cholesterol rich diet and the drug Atorvastatin 5mg/kg. Group IV received cholesterol rich diet and the drugLutein 25mg/kg. Group V received cholesterol rich diet and the drugLutein 50mg/kg. Group VI received cholesterol rich diet and the drug Lutein100mg/kg. At the end of 16 weeks, Blood samples from each rats was taken through retro-orbital puncture to evaluate serum lipoproteinsand lipoprotein(a) and thenwistar rats were sacrificed underinjection I.M Ketamine,Aortaand Liverwere dissected out and sent for histopathological studies. Results The plasma LDL, VLDL, Triglycerides, total cholesterol, lipoprotein(a) levels were reduced in all lutein treated groups and atorvastatin treated group compared to high cholesterol diet group. A significant rise in HDL levels was noted in all Lutein treated groups and atorvastatin treated group. No statistically significant difference was seen between Atorvastatin 5mg/kg body weight and Lutein 100mg/kg body weight on reduction of total cholesterol.The efficacy of the drug Lutein in progression of atherosclerosis and its cytoprotective action in liver was proved in this study. Conclusion This study indicates that Lutein has effect onreducing plasma lipoproteins&the study had shown significant antiatherogenic effect.

scholarly journals Effects of Oral Administration of Monosodium Glutamate (MSG) on Obesity in Male Wistar Rats (Rattus Norvegicus)

2021 ◽  
Vol 5 (4) ◽  
pp. 851-854
Author(s):  
Mulyati Sri Rahayu ◽  
Sri Wahyuni ◽  
Yuziani
Keyword(s):  
Body Weight ◽  
Adult Male ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Body Weight Gain ◽  
Monosodium Glutamate ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Group 2

Introduction: Monosodium glutamate (MSG) is one of the most widely employed food enhancers. Although the umami compound, controversy persists regarding the effects of MSG intake on body weight. Chronic MSG intake may result in excessive body weight gain and obesity. Consumption of MSG result in organ damage, cardiovascular disease, oxidative stress, and also risk factors for obesity. This study aims to determine the effect of oral MSG on obesity in adult male Wistar rats (Rattus norvegicus).Methods: This true experimental study used the post-test control group design. Twenty-four adult male Wistar rats were randomly divided into four groups: control (received distilled water), Group 1 (MSG 0.378 mg/gr BW), Group 2 (0.756 mg/gr BW) and Group 3 (1.512 mg/gr BW). The obesity parameter was obtained by the Lee index. Kruskal-Wallis test follows by Mann-Whitney test were used to compare the Lee index between groups.Results: Lee’s index mean for each group was 358.4%, 314.1%, 287.8%, and 320.9%, respectively. The Kruskal Wallis test showed a significant difference in the Lee index between groups (p = 0.043). A follow-up test using Mann-Whitney found a significant difference between group 2 and the control group (p = 0.043, p <0.05). The mean of Lee index of group 2 was 70.51% lower than the control group.Conclusion: This study concluded that Lee index was not increased in MSG-treated rats than in the control group after oral MSG intervention for 21 days.

scholarly journals Efek Pemberian Minyak Atsiri Daun Sirsak (Annona muricata Linn) terhadap Penurunan Kadar Tumor Necrosis Factor Alpha (TNF-α) Hepar Tikus Wistar Jantan yang Diinduksi Rifampisin

2018 ◽  
Vol 7 (01) ◽  
Author(s):  
Shofiyyatunnisa Wuslatush Sholihah ◽  
Marindra Firmansyah ◽  
Dini Sri Damayanti
Keyword(s):  
Essential Oil ◽  
Tumor Necrosis ◽  
Wistar Rats ◽  
Corn Oil ◽  
Control Group ◽  
Annona Muricata ◽  
Necrosis Factor Alpha ◽  
Tnf Α ◽  
Significant Difference ◽  
Male Wistar Rats

 AbstractIntroduction: Rifampicin is one of anti-tuberculosis drugs are reported to have hepatotoxic effects through increasing ROS (Reactive Oxygen Species) so thattriggering necrosis of liver cells and stimulates the formation of TNF-α. Essential oil of Annona muricata Linn. contains linalool, β-caryophyllen, trans-caryophyllen, germacrene A, germacrene B, germacrene D, sesquisterpene, β-canidine, γ-canidine. The objective of this study is to investigate the effect of essential oil of Annona muricata Linn. on levels of Tumor Necrosis Alpha(TNF-α) liver in male wistar rats which induced by rifampicin.Methods: This was an invivo experimental study with control group post test only design and use 25 male wistar rats and divided into 5 groups, they are KN (corn oil), KP (Rifampicin), P1 (Rifampicin + Essential oil 1,5%), P2 (Rifampisin + Essential oil 3%), P3 (Rifampisin + Essential oil 6%) for 6 weeks and then levels of TNF-α liver will be checked. Analysis of the data using One Way (ANOVA) test and then tested by post hoc test using Least Significant Difference (LSD). The result significant if p<0,05.Result: Induced rifampicin can increased levels of TNF-α liver than group KN (p<0,05). Treatment of essential oil of Annona muricata Linn. on P1, P2, and P3 can reduced levels of TNF-α liver than group KP (p<0,05). Allegedly this is due to the active ingredient in the essential oils act as antioxidants and antiinflammatory.Conclusion: Essential oil of Annona muricata Linn. at all doses can reduced levels of TNF-α liver in male wistar rats which induced by rifampicin.Keywords:rifampicin,essential oil of Annona muricata Linn., TNF-α liver

scholarly journals THE EFFECT OF RAMANIA LEAF (Bouea macrophylla Griff) EXTRACT GEL ON COLLAGEN FIBERS DENSITY IN INCISIONAL WOUND OF MALE WISTAR RATS

2021 ◽  
Vol 6 (1) ◽  
pp. 96
Author(s):  
Dinda Rizkia ◽  
Irham Taufiqurrahman ◽  
Renie Kumala Dewi
Keyword(s):  
Wistar Rats ◽  
Leaf Extract ◽  
Collagen Fibers ◽  
The Body ◽  
Control Group ◽  
Control Group Design ◽  
Treatment Groups ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Post Hoc

ABSTRACTBackground: Ramania leaf (Bouea macrophylla Griff) extract gel has secondary metabolites in the form of flavonoids, steroids, phenols and terpenoids which have a role as antioxidant. They will protect the body from excessive production of reactive oxygen species (ROS) by increasing endogenous antioxidants SOD, CAT and GPX, so that wound healing will not be inhibited and the process of collagen synthesis can run smoothly. Objective: To analyze the effect of ramania leaf extract gel that was applied topically with 5%, 10% and 15% concentration on collagen fibers density in incisional wound of male Wistar rats (Rattus norvegicus) on the 7th and the 14th day. Method: This research is a pure experimental study with a posttest-only control group design, using 24 rats which were divided into 4 groups: the treatment groups given ramania leaf extract gel of 5%, 10%, 15%, and the control group given placebo gel. The application of the extract gel was done once within 24 hours. The collagen level was measured with a spectrophotometer on the 7th and the 14th day. Results: Two-Way ANOVA test results on the 7th and the 14th day of each group showed a significant difference with p=0.000 (p<0.05). The Bonferroni Post-hoc Test showed a significant difference with p<0.05 between the placebo gel group and the groups of ramania leaf extract gel of 5%, 10%, 15% on the 7th and the 14th day. Conclusion: There is an effect of ramania leaf extract gel on collagen fibers density with the most effective concentration of 15%.Keywords: Antioxidant, , Collagen, Ramania Leaf Extract Gel

scholarly journals The Effect of Paracetamol and Codeine Analgesic Combination on Creatinine Levels in Male Wistar Rats

2020 ◽  
Vol 1 (1) ◽  
pp. 5-9
Author(s):  
Vianney Natasaputra ◽  
Taufik Eko Nugroho
Keyword(s):  
Body Weight ◽  
Statistical Analysis ◽  
Pain Management ◽  
Serum Creatinine ◽  
Wistar Rats ◽  
Control Group ◽  
Control Group Design ◽  
Significant Difference ◽  
Post Test ◽  
Male Wistar Rats

Background :  Pain is one of the most common complaint in patients. Combination of paracetamol and codeine is an alternative analgesic combination in chronic pain management. They belong to different group of analgesic and have different mechanism of action. Combination of these drugs give a better potential in pain management.  However, these drugs also have potential side effect on the kidney.Methods : An experimental study of post-test only control group design. The sample were 20 Wistar rats, randomized into 4 groups: control group, a group receiving paracetamol 32 mg/kg body weight, a group receiving codeine 1,9 mg/kg body weight, and a group receiving paracetamol 32 mg/kg body weight and codeine 1,9 mg combination, administered 4 times a day orally using gastric instillation for 28 days. At the 29th day, blood is collected from retro orbital vessel to measure the serum creatinine levels. Statistical analysis was conducted using ANOVA TestResult : Obtained from statistical analysis there is no significant difference in serum creatinine levels in Wistar rats given all treatment group (p > 0,05).Conclusion : There is no significant difference in serum creatinine levels between administration of paracetamol and codeine combination compared to the control group.Keywords : Paracetamol, Codeine, Creatinine Serum, Pain

scholarly journals Some Effects of Crude Aqueous Extracts of Hibiscus sabdariffa Leaves on the Testes and Sperm Parameters of Adult Male Wistar Rats (Rattus norelegicus)

2019 ◽  
pp. 1-8
Author(s):  
A. Ashamu Ebenezer ◽  
A. Oyeniran David ◽  
T. Awora Koyinsola ◽  
O. T. Olayemi ◽  
O. Oyewo Olutoyi
Keyword(s):  
Body Weight ◽  
Adult Male ◽  
Wistar Rats ◽  
The Body ◽  
Male Rats ◽  
Control Group ◽  
Testis Weight ◽  
Hibiscus Sabdariffa ◽  
Male Wistar Rats ◽  
Group D

Hibiscus sabdariffa is a common garden plant native to warm-temperate, subtropical and tropical regions throughout the world, used in traditional medicine. It is mostly cultivated for its flowers. The calyces are used as a refrigerant in form of tea (especially in making the sorrel drink popularly known as ‘zobo’ in Nigeria), jellies and jam. Aim: This study evaluates the effects of aqueous leaves extract of Hibiscus sabdariffa on the body, testis weight, histology of the testes and sperm parameter of adult male Wistar rats. Methodology: Twenty-five adult male wistar rats of 10-12 weeks and weighing about 120-140g were divided into four groups (A, B, C and D) (five rats / group), Group A was kept as control and B, C, D were administered 250 mg, 500 mg, 1000 mg  of Hibiscus sabdarfia leaf extract per Kg body weight for 8 weeks. The rats in each group were fed with rat feed and water ad libitum. Administration was by means of an oral cannula. At the end of each experimental period, the rats were sacrificed by cervical dislocation, the testes were harvested and immediately fixed in Bouin’s fluid for histological procedure. However, as soon as the animals were sacrificed, the cauda epididymis was removed and semen analysis was carried out immediately. Results: There was a significant decrease in body weight of all the test groups at (P <0.05) and a significant decrease in the testes of the rats in group D when compared with the control group. Statistical analysis revealed decrease in sperm count, motility and viability with a significance decrease (P <0.05) in group D only as compared to control group. The histoarchitecture revealed significant degenerative changes characterized by vacuolization in the intestitium and seminiferous epithelium when compared with the control group. Conclusion: The aqueous leaves extract of Hibiscus sabdariffa appears to have adverse effects on the fertility of male rats especially when taken over a long period of time.

scholarly journals PENGARUH PEMBERIAN EKSTRAK KULIT PISANG KEPOK KUNING (Musa balbisiana) TERHADAP ASUPAN MAKAN DAN BERAT BADAN TIKUS WISTAR (Rattus norvegicus) JANTAN

2017 ◽  
Vol 4 (3) ◽  
pp. 193
Author(s):  
Wiwiek Fatchurohmah ◽  
Andreanyta Meliala
Keyword(s):  
Body Weight ◽  
Food Intake ◽  
Wistar Rats ◽  
Overweight And Obesity ◽  
Banana Peel ◽  
Positive Energy ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Peel Extract

A positive energy imbalance results in overweight and obesity. Overweight and obesity are the major risk factors for many diseases, including diabetes, cardiovascular diseases, and mental health. Animal studies suggested that serotonin involves in food intake control. The Banana peel is a potential natural source of serotonin precursor; furthermore, it may influence food intake. This study aimed to determine the effect of banana peel extract on food intake and body weight gain in male Wistar rats. Twenty-four male Wistar rats (8-10 weeks old), were divided into four groups for feeding treatment. All four group were; the control group (C), the banana peel extract dosage of 4 g/kg BW group (T1), the banana peel extract dosage of 8 g/kg BW (T2), and the banana peel extract dosage of 16 g/kg BW (T3). The food intakes were measured daily, while rats body weights were recorded at the beginning and the end of the three days experimental periods. The study found the rats in the T1 group (8.80 ± 2.63) showed no significant difference in food intake compared to C group (10.68 ± 1.89), T2 group (6.65 ± 3.12), and T3 group (5.42 ± 1.59). Rats in the T2 group (p = 0.038) and T3 group (p = 0.005) showed lower food intake significantly compared to control group. After three days of treatment, the T3 group showed a significant decrease in body weight compared to control group (p = 0.008). This study concluded, the administration of banana peel extract dosage of 8 g/kg BW and 16 g/kg BW suppressed food intake, while the dosage of 16 g/kg BW reduced the body weight in male Wistar rats.

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