Evaluation of Exogenous Laccase Enzyme Treated Finger Millet Straw on Body Weight Gain, DM Intake and Nutrient Digestibility in Heifers

Author(s):  
Vidya Pradeep Kumar ◽  
Ramya G. Rao ◽  
A. Dhali ◽  
Vandana Thammiaha ◽  
Manpal Sridhar

Background: Fungal laccases have widespread application in a number of biotechnological processes including the biodegradation of lignin. Their low yield in the native state limits their practical use in the deconstruction of lignocellulosic biomass for feeding ruminants. Enzymes in bulk quantities are required to treat biomass which has got greater product consistency and less lot to lot variations. The present study was an attempt to evaluate the effect of feeding finger millet straw treated with exogenous laccases in heifers.Methods: The effect of feeding exogenous laccases obtained from immobilized Schizophyllum commune (MTCC 11893) on body weight gain, dry matter intake and nutrient digestibility in heifers was evaluated in three groups of heifers (4-each) with body weights ranging between 275 ± 47.12 to 276.75 ± 64.48kg. The control group received ad lib. finger millet straw treated with only production media (GI). Test group 1 (GII) received ad lib. straw treated with laccase rich media in a 3:5 (w/v) ratio, while test group 2 (GIII) received ad lib. straw treated in a 4:5 (w/v) ratio. Result: After 14- days of feeding, gain in body weights for GI, GII and GIII were 277.9 ± 68.47; 277.50 ± 46.43 and 278.85 ± 37.22 respectively with an overall increase of 1.15, 4.25 and 3.60 kgs. No significant variation (P greater than 0.05) was observed with regard to DMD (%) between the groups which was 46 ± 5.8 for the control animals and 41±13.0 and 41± 3.4 in G2 and G3 groups. Though significant variations were observed digestibility studies proved inconclusive. Though preliminary results indicate that applying lignin degrading enzymes as feed supplements could enhance digestibility of crop residues in ruminants.

2014 ◽  
Vol 84 (1-2) ◽  
pp. 5-11 ◽  
Author(s):  
Eun Y. Jung ◽  
Sung C. Jun ◽  
Un J. Chang ◽  
Hyung J. Suh

Previously, we have found that the addition of L-ascorbic acid to chitosan enhanced the reduction in body weight gain in guinea pigs fed a high-fat diet. We hypothesized that the addition of L-ascorbic acid to chitosan would accelerate the reduction of body weight in humans, similar to the animal model. Overweight subjects administered chitosan with or without L-ascorbic acid for 8 weeks, were assigned to three groups: Control group (N = 26, placebo, vehicle only), Chito group (N = 27, 3 g/day chitosan), and Chito-vita group (N = 27, 3 g/day chitosan plus 2 g/day L-ascorbic acid). The body weights and body mass index (BMI) of the Chito and Chito-vita groups decreased significantly (p < 0.05) compared to the Control group. The BMI of the Chito-vita group decreased significantly compared to the Chito group (Chito: -1.0 kg/m2 vs. Chito-vita: -1.6 kg/m2, p < 0.05). The results showed that the chitosan enhanced reduction of body weight and BMI was accentuated by the addition of L-ascorbic acid. The fat mass, percentage body fat, body circumference, and skinfold thickness in the Chito and Chito-vita groups decreased more than the Control group; however, these parameters were not significantly different between the three groups. Chitosan combined with L-ascorbic acid may be useful for controlling body weight.


2013 ◽  
Vol 49 (1) ◽  
pp. 135-148 ◽  
Author(s):  
Fernando Márlisson de Queiroz ◽  
Kayo Wanderson de Oliveira Matias ◽  
Mylena Mylana Freire da Cunha ◽  
Aline Schwarz

Phyllanthus niruri L. (Euphorbiaceae), known as "quebra-pedra" (Portuguese for "stonebreaker"), is an herb used for kidney disorders. In light of its frequent use by the population, the present study aimed to investigate the genotoxic, antigenotoxic and cytotoxic activities of a standardized P. niruri extract in bone marrow rats. Three groups of 12 animals were treated daily by gavage over a period of 30 days, with 50, 150 or 250 mg/kg of P. niruri extract aqueous solution. The control group (n = 12) received tap water. At the end of treatment (day 31), groups were divided into two minor subgroups (n=6/group) and received cyclophosphamide (50 mg/kg, i.p.) or saline 0.9% (i.p.). After 24 hours, we evaluated the frequency of micronucleated polychromatic erythrocytes for each animal (MNPCE) at 1000 PCE. Cytotoxicity was evaluated with the PCE/NCE ratio (NEC = normochromatic erythrocytes). General toxicity was assessed during treatment using the parameters of body weight gain, ration and water consumption. The dry extract did not provoke changes in body weight, weight gain, ration and water intake or changes in the frequency of MNPCE or cytotoxicity in bone marrow. We propose that the P. niruri extract used here showed no genotoxic, antigenotoxic and cytotoxic activities under the experimental conditions.


2020 ◽  
Vol 17 (35) ◽  
pp. 800-812
Author(s):  
Ilgiz DOLININ ◽  
George BAZEKIN ◽  
Evgeny SKOVORODIN ◽  
Almaz SHARIPOV ◽  
Ivan CHUDOV

Poultry farming holds a special place in ensuring the products that the consumers demand, it provides the population with essential food products,such as eggs and meat,that contain vital micro and macronutrients, proteins, lipids, and vitamins. Therefore, the issues of rational, economically feasible feeding of meat poultry, namely broiler chickens, are an urgent task. It is also essential to find effective methods of their application in order to correct the natural resistance and immune and biological reactivity of birds. The purpose of this research is to study the effect of the biological stimulant-Nucleostim on the growth and development of chickens, hematological, and immunological parameters of the blood of birds.This Biostimulant is a purified bovine spleen extract containing at least 1 mg / ml of low molecular weight peptides (nucleotides and nucleosides) formed as a result of autolysis, using dry whey and diatomite as fillers. Onthe application ofNucleostim, the gain in live weight of chickens was increased by 9.7%. At the end of the experiment, the livability of the chicks of the experimental group treated with Nucleostimcame up to 88%, compared with the 72% of the control group. The use of biostimulant had a stimulating effect on the liver of chickens confirmed by the research results presented in the article, as well as contributed to the development of the thymus in the setting of general dystrophy. Thus, it improved chicklivability and increased body weight gain. The biological stimulant-Nucleostim as an adaptogenic, anabolic, and immunostimulatory agent is promising for finding new drugs that improve the health and productivity of poultry.


2018 ◽  
Vol 18 (4) ◽  
pp. 1007-1027 ◽  
Author(s):  
Małgorzata Świątkiewicz ◽  
Jerzy Księżak ◽  
Ewa Hanczakowska

AbstractThe aim of this experiment was to examine the possibility of replacing part of soybean meal in the diets of sows, their piglets and fatteners with two varieties of high- or low-tannin faba bean seed. Twenty-four sows were allocated to 3 groups, 8 animals in each. Control group (C) received standard feed mixture containing soybean meal as the main protein source. Next groups received standard feed mixture in which part of soybean meal was replaced by high-tannin variety Bobas (group HT) or low-tannin variety Kasztelan (group LT). Faba bean was added to diets at the level of 12 and 14% for pregnant and lactating sows, 6% for piglets, and 12 and 16% for fatteners (grower and finisher, respectively). Diets for half piglets and fatteners were supplemented with the enzyme Ronozyme VP. Apparent digestibility of nutrients was evaluated in a parallel experiment, using the same feeds on 18 barrows weighing about 40 kg (grower) and 80 kg (finisher). There was almost no difference in sow reproductive rates, litter weight and body weight of piglets on the first day of life were similar. Until 35 days of age piglets receiving faba bean grew faster than control ones. Control piglets grew fastest from 35 to 84 days of age, but differences were not significant. Enzyme supplement had a positive effect on body weight only from 56 to 84 days of piglet life. Digestibility of nutrients was lower in pigs fed with faba beans, especially in the grower period. Faba bean HT had lower nutrient digestibility in both periods of fattening. Almost all carcass traits were better in control pigs than in those fed with bean variety Bobas. Meat of these pigs had also the lowest content of unsaturated fatty acids and highest atherogenic indices. In sensory evaluation this meat had also the worst smell and taste. It can be stated that faba bean seeds, in moderate amounts, can partially replace soybean meal in feed for pigs, but some lowering of body weight gain and meat quality is possible especially when high-tannin varieties are used.


2013 ◽  
Vol 42 (1) ◽  
pp. 40-43 ◽  
Author(s):  
S Yesmin ◽  
ME Uddin ◽  
R Chacrabati ◽  
M Al-Mamun

The present study was conducted to evaluate the effect of different levels of methionine supplementation on feed intake, nutrient digestibility and growth performance of growing rabbit. Sixteen weaned crossbred New Zealand White (NZW) growing rabbits (30-35 d) were distributed into four treatment groups having four replications in each group using a Completely Randomized Design (CRD). Basal diet composed of green grass (dhal grass) and concentrate mixture which was offered ad libitum basis for 56 days period. Four levels of methionine such as 0.0% (control), 0.15%, 0.25%, and 0.35% were supplemented randomly to rabbits. Results showed that supplementation of methionine did not affect green grass intake. Cumulative as well as daily concentrate and DM intake were significantly (p<0.05) higher for all methionine groups than control group. Final body weight gain as well as daily, weekly and cumulative body weight gains were improved significantly with increasing level of methionine. It was found that methionine had significant (p<0.01) effect on digestibility of DM, CP, NFE and EE but CF digestibility did not differ significantly. Digestibility was improved with increasing the level of methionine. Feed conversion ratio also decreased significantly with methionine supplementation, and 0.25% methionine group showed the best performance among the four treatments. DOI: http://dx.doi.org/10.3329/bjas.v42i1.15777 Bang. J. Anim. Sci. 2013. 42 (1): 40 43


2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Abdallah E. Metwally ◽  
Ahmed A. A. Abdel-Wareth ◽  
Ahmed A. Saleh ◽  
Shimaa A. Amer

Abstract Background The aim of this study is to investigate whether the energy matrix values of the nonstarch polysaccharide- (NSP-) degrading enzymes, bioemulsifier (LYSOFORTE®), guanidinoacetic acid (CreAMINO®), or their combinations could be summed. The effects of these additives on the growth performance, carcass traits, and economic value of the broiler chicken diets were evaluated. A total of 525-one-day-old Ross chicks with an initial body weight of 42.96 ± 0.87 g were haphazardly allocated into seven groups with five replicates. The seven experimental treatments are as follows: (1) basal diet with no additives (breeder recommendation), which is the control group, (2) basal diet minus 100 kcal/kg supplemented with 0.02% NSP-degrading enzymes (NSP), (3) basal diet minus 50 kcal/kg supplemented with 0.025% emulsifier (LYSOFORTE®), (4) basal diet minus 50 kcal/kg supplemented with 0.06% guanidinoacetic acid (CreAMINO®), (5) basal diet minus 150 kcal/kg supplemented with a mixture of NSP and LYSOFORTE® (NSPL), (6) basal diet minus 100 kcal/kg supplemented with a mixture of NSP and CreAMINO® (NSPC), and (7) basal diet minus 200 kcal/kg supplemented with a mixture of NSP, LYSO, and CreAMINO® (NSPLC). The experiment lasted for 35 days. Results It was found that the final body weight, body weight gain, and relative growth rate were significantly higher in birds fed diets supplemented with NSPL, NSPC, CreAMINO, and LYSO with the reduced energy matrix value. The overall feed conversion ratio was significantly improved due to the supplementation of NSPC, CreAMINO, NSPL, and LYSO with the reduced energy matrix value compared to the control group. Moreover, no significant effect on the carcass criteria was observed by the different treatments. As a result of the dietary supplementation with NSPL, NSPC, CreAMINO®, and LYSO with the reduced energy matrix value, the net profit, total return, economic efficiency, and performance index were increased and the cost of feed per kg of body weight gain was decreased. Conclusion The energy matrix value of NSPL, NSPC, CreAMINO®, and LYSOFORTE could be established in the diets of broiler chickens to improve the growth performance and economic efficiency.


2014 ◽  
Vol 11 (1) ◽  
pp. 1-5 ◽  
Author(s):  
S Khatun ◽  
M Mostofa ◽  
F Alom ◽  
J Uddin ◽  
MN Alam ◽  
...  

The efficacy of tulsi (Ocimum sactum) and neem (Azadirachta indica) leaves extract as a growth promoter were studied in broiler. A total of 40 day-old broiler chicks were purchased from Kazi hatchery and after three days of acclimatization the chicks were randomly divided into four groups (n=10). No vaccination schedule was practiced and no antibiotic was added in ration of group A, B, C, and D respectively. Group A served control without any supplements while group B, C and D were supplemented with combination of tulsi and neem extract @ 1 ml, 2ml and 3 ml/liter of drinking water. Live body weight gain was recorded weekly up to 6th weeks and hematological studies were performed at 21st and 42nd day of experiments. At the end of 42nd day of experiment final body weight of group A, B, C and D were 1561± 12.10 g, 1698± 12.87 g, 1608± 12.04 g and 1763± 13.28 g, respectively. The birds of group D utilized their feed more efficiently among the treatment groups (p<0.05). The net body weight gain were, 1533± 11.98 g, 1443± 11.32 g and 1588± 12.10 g in treated groups compared to control group (1393± 11.07 g ) and total net profit per broiler was 19.08, 40.10, 20.68 and 45.07 Taka , respectively. Hematological parameterss (TEC, PCV, Hb and ESR) were not significantly changed among the treated and control group suggesting no side effects of herbal extracts in broiler. It can be concluded that tulsi and neem extract is economic and safe in broiler production.DOI: http://dx.doi.org/10.3329/bjvm.v11i1.17728 Bangl. J. Vet. Med. (2013). 11 (1): 1- 5


2020 ◽  
Vol 6 (2) ◽  
pp. 168-175
Author(s):  
Atikur Rahaman ◽  
Md Bazlar Rashid ◽  
Md Mahmudul Hasan ◽  
Rakibul Islam ◽  
Fahima Binthe Aziz ◽  
...  

This study was conducted under the Department of Physiology and Pharmacology to determine the effects of Neem and Nishyinda leaves supplementation on growth performance and GUT biosis in broilers. A total of 40 day old broiler chicks were divided into four groups A, B, C and D. Group A was considered as control, fed only with commercial broiler ration. Group B supplemented with formulation of 2 gm grinded Neem leaves, Group C with 2 gm grinded Nishyinda leaves and Group D with 1 gm grinded Neem leaves plus 1 gm grinded Nishyinda leaves per liter of water respectively. Observations were recorded for live body weight, weight gain, feed consumption, feed efficiency, hemato-biochemical parameters and GUT biosis specially the parasite of birds in six weeks. Body weights were increased significantly (p<0.05) in all treated groups in respect to the control and highest was recorded in combine Neem plus Nishyinda supplemented groups (Group D). No significant (p>0.05) differences were observed among the groups for PCV and TEC values. Hb content increased and ESR decreased significantly (p<0.05) with combine Neem plus Nishyinda supplemented group (Group D). The serum SGOT and SGPT levels were also decreased with the same kind of supplementation. In treatment group there was significantly decreased of parasitic eggs, but in case of control group no decrease of parasitic eggs. There was no significant pathological change in any internal organs of the broiler of treated groups. Best result was found in the group D. The present study reveals that combine supplementation of Neem plus Nishyinda gives better result over other groups in respect to body weight gain, feed conversion ratio, hemato-biochemical parameter and profitability without making any health hazard of the broilers. Asian J. Med. Biol. Res. June 2020, 6(2): 168-175


2021 ◽  
Vol 34 (2) ◽  
pp. 265-275 ◽  
Author(s):  
Y. C. Hsieh ◽  
W. C. Lin ◽  
W. Y. Chuang ◽  
M. H. Chen ◽  
S. C. Chang ◽  
...  

Objective: The study developed mushroom stalk residues as feed additives in the broiler diet for improving the growth performance and immunity of broilers as well as to increase the value of mushroom stalk residues.Methods: In total, 300 ROSS 308 broilers were randomly allocated into fifteen pens with five dietary treatments: i) control, basal diet; ii) CMWM, supplemented with 1% <i>Cordyceps militaris</i> waster medium (CM); iii) CMPE, supplemented with 0.5% CM+0.5% <i>Pleurotus eryngii</i> stalk residue (PE); iv) CMPS, supplemented with 0.5% CM+0.5% <i>Pleurotus sajorcaju</i> stalk residue (PS); v) CMFV, supplemented with 0.5% CM+0.5% <i>Fammulina velutipes</i> stalk residue (FV).Results: The chemical analysis results showed that CM extracts, PE extracts, PS extracts, and FV extracts contain functional components such as polysaccharides and phenols and have both 2, 2‐diphenyl‐1‐picryl‐hydrazyl‐hydrate scavenging and Ferrous scavenging capacities. The group CMWM saw increased body weight gain and feed conversion rate and the promotion of jejunum villus growth, but there is no significant difference in the intestinal bacteria phase. Antioxidant genes in the nuclear factor (erythroid-derived 2)-like 2 (Nrf2)- antioxidant responsive element pathway among the groups are significantly higher than that of the control group, especially in group CMWM.Conclusion: The mushroom stalk residues have antioxidant functional components, can improve the intestinal health and body weight gain of chickens, and can activate the antioxidant pathway of Nrf2 to increase the heme oxygenase-1 expression. The treatment with 1% CM was the most promising as a feed additive.


2012 ◽  
Vol 12 (2) ◽  
pp. 49-54
Author(s):  
Ni Wayan Siti ◽  
I. G. M. A. Sucipta ◽  
I. M. Mudita ◽  
I. B. G. Partama ◽  
I.G.L.O. Cakra

The supplementation of urea molasses block for increasing performance of ettawah crossbred goats fed diet containing gliricidia spium green forage ABSTRACT. The aimed of this experiment to study the supplementation of urea molasses block for performance increased of ettawah crossbred goats fed diet containing Gliricidia spium green forage has been conducted at Tabanan regency and Animal Nutrition Laboratory. The Latin Square Design which four treatments and four replicates was used in this experiment. The four treatments were the levels of urea molasses block : 0, 50, 100 and 150g respectively. The animal access to ad libitum Gliricidia spium green forage and drink water. The animal fed twice a day urea molasses block at the morning and at the afternoon. The variables which were observed including feed consumption, nutrient digestibility, body weight gain and feed conversion ratio. The result of this experiment showed dry matter feed consumption, drink water, nutrient digestibility of the four treatments is not significantly different (P 0.05). The body weight gain at treatment C was significantly higher (P 0.05), but feed conversion ratio (FCR) was significantly lower (P 0.05) than treatments A, B and D. From the results in this experiment it can be concluded that the used of urea molasses block 100g on the effect to increased the performance of ettawah crossbred goats with basal diet Gliricidia spium green forage.


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