Effect of iron source on iron deficiency induced chlorosis in groundnut

2015 ◽  
Author(s):  
Anita Mann ◽  
A. L. Singh ◽  
Shubhangi Oza ◽  
Nisha Goswami ◽  
Deepti Mehta ◽  
...  
Keyword(s):  
Iron Deficiency ◽  
Nitrate Reductase ◽  
Chlorophyll Content ◽  
Reductase Activity ◽  
Foliar Spray ◽  
Absorption Capacity ◽  
Iron Deficiency Chlorosis ◽  
Iron Source ◽  
Fe Content ◽  
Ion Absorption

In a field study, the effect of iron source through foliar as well as basal application was studied on lime induced iron-deficiency chlorosis (LIIC), chlorophyll content, nitrate reductase activity, available Fe and micronutrients in groundnut. The visual chlorotic rating screen of various Fe-efficient and Fe-inefficient cultivars clearly identified Fe-efficient and Fe-inefficient. Tirupati-4 was showing symptoms of chlorosis and was Fe-inefficient. Fe absorption capacity varied among cultivars. Applications of iron increased active Fe content in LGN-2 by 5.6 % and 163.18% in CSMG-84-1 respectively. A significant increase in chlorophyll content (10%) and nitrate reductase (110%) was observed with foliar spray of FeSO4. A significant damage of lipid peroxidation was observed in absence of iron which was improved by 37% in Tirupati-4 and 16.67% in CSMG-84-1 by foliar and basal supplementation of Fe, respectively. A strong correlation among the Fe, Mn, Zn and K depicted ionomic interaction with different treatments. Based on the ion absorption capacity and the level of chlorosis, the groundnut genotypes were grouped as tolerant, moderately tolerant and sensitive to iron chlorosis.

Iron Deficiency Chlorosis and Seed Yield in Dry Edible Beans Grown on High-pH Calcareous Soils

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 444f-445
Author(s):  
J.W. Gonzales ◽  
D.P. Coyne ◽  
W.W. Stroup
Keyword(s):  
Iron Deficiency ◽  
Chlorophyll Content ◽  
Seed Yield ◽  
Calcareous Soils ◽  
Yield Reduction ◽  
Iron Deficiency Chlorosis ◽  
Breeding Lines ◽  
High Ph ◽  
The Third ◽  
Content Index

Iron deficiency chlorosis (FeDC) can cause significant seed yield reduction in dry beans (Phaseolus vulgaris L.) grown on high-pH calcareous soils. To determine the effects of FeDC on seed yield, and the effect of Fe-spray as a correction factor for FeDC, 22 breeding lines/cultivars were planted on high-pH (8.0), calcareous (3.2–3.5 calcium carbonate equivalent), and low-Fe (1.8–4.2 ppm DTPA) sandy clay loam Tripp soils at Mitchell and Scottsbluff in western Nebraska. A split-plot design was used with Fe treatments as main plots and breeding lines/cultivars as subplots. Three foliar sprays of Fe-EDDHA (2.4 kg·ha–1) were applied at V4, R5, and R7 dry bean growth stages, during 1996 and 1997. Leaf chlorosis was measured simultaneously by using a Minolta Chroma-meter (CIE L* a* b* color space system), a Minolta Chlorophyll-meter (chlorophyll content index), and by visual ratings (1 = normal green to 5 = severe chlorosis). In 1996 no significant Fe-spray × line interaction (P = 0.776) and Fe-spray effect (P = 0.884) on seed yield was observed. Breeding lines showed significant differences in seed yield (P = 0.0001) with WM2-96-5 being the highest-yielding line (4047 kg·ha–1). In 1997 a significant Fe spray × line interaction (P = 0.029) was observed. The cultivar Chase without Fe spray (3375 kg·ha–1), and lines WM2-96-5 (3281 kg·ha–1), WM2-96-8 (3171 kg·ha–1) with Fe spray were the highest yielding entries under those treatments. Differences in visual ratings after the third Fe spray in 1997 were significant (P = 0.004) for Fe spray × line interaction. In 1996 visual ratings were different only for breeding lines. Chlorophyll content index showed a significant Fe spray × line interaction after the second Fe spray (P = 0.022) and after the third Fe spray (P = 0.0003) in 1997.

scholarly journals Physiological responses of bio-silica-treated oil palm seedlings to drought stress (Tanggap fisiologi bibit kelapa sawit yang diberi bio-silika terhadap cekaman kekeringan)

2019 ◽  
Vol 87 (1) ◽  
Author(s):  
Dian Mutiara AMANAH ◽  
Nurhaimi HARIS ◽  
Laksmita Prima SANTI
Keyword(s):  
Drought Stress ◽  
Nitrate Reductase ◽  
Chlorophyll Content ◽  
Oil Palm ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Stomatal Closure ◽  
Growth Stimulation ◽  
Biotic And Abiotic Stress ◽  
Proline Concentration

Silica (Si) in the form of soluble silicic acid [H4SiO4] was an element that makes plants more resistant to drought stress through biochemical or molecular processes and contributing to growth stimulation under biotic and abiotic stress conditions. The objective of this study was to determine the response of oil palm seedlings to drought stress by the bio-Si application. The experiment was arranged in complete random design (CRD) with ten replicates.  Bio-Si was developed in solid and liquid forms with a dissolved Si content at least 10% (w/v). The eight combinations of solid bio-Si application per seedling were: (i) blank (without fertilizers), (ii) 5 g NPK 15-15-15, (iii) 5 g NPK 15-15-15 + 109cfu of Si-solubilizing microbes (SSM), (iv-viii) 5 g NPK 15-15-15 + 2.5; 5.0; 7.5; 10 g bio-Si; and 5 g Na2SiO3.  On the other hand, liquid bio-Si application per seedling were: (i) blank (without fertilizers), (ii) 5 g NPK 15-15-15, (iii) 5 g NPK 15-15-15 + 109cfu of SSM, (iv-viii) 5 g NPK 15-15-15 + 25 mL; 50 mL; 75 mL; 100 mL bio-Si; and 50 mL Na2SiO3. Drought stress tolerance was analyzed by using proline concentration, nitrate reductase activity (NRA), chlorophyll content, and stomatal closure in the leave of oil palm seedlings. Based on the physiological response, this research indicates that bio-Si application could induce seedling tolerance to drought stress. The bio-Si treatments gave a positive response of proline concentration, nitrate reductase activity (NRA), chlorophyll content, and stomatal closure. The doses of 5 g NPK 15-15-15 + 7.5 g solid bio-Si and 5 g NPK 15-15-15 + 75 mL liquid bio-Si per seedling were a recommended to increase oil palm seedlings tolerance to drought stress.[Key words: bio-Si, chlorophyll, nitrate reductase activity, Si-solubilizing microbes]. AbstrakSilika (Si) dalam bentuk terlarut asam silikat [H4SiO4]merupakan unsur yang dapat menyebabkan tanaman lebih tahan terhadap cekaman kekeringan melalui proses biokimia atau molekuler dan menstimulasi pertumbuhan dalam kondisi cekaman biotik dan abiotik. Tujuan dari penelitian ini adalah mengetahui respons fisiologi bibit kelapa sawit yang diberi bio-Si terhadap cekaman kekeringan. Penelitian didesain dengan rancangan acak lengkap (RAL) dan sepuluh ulangan. Bio-Si dikembangkan dalam bentuk padat dan cair dengan kadar Si terlarut minimal 10 % (b/v). Delapan aplikasi bio-Si padat per bibit adalah: (i) blanko (tanpa pupuk), (ii) 5 g NPK 15-15-15, (iii) 5 g NPK 15-15-15 + 109cfu mikrob pelarut silika, (iv-viii) 5 g NPK 15-15-15 + 2,5 g; 5,0 g; 7,5 g; 10 g bio-Si, dan 5 g Na2SiO3. Sementara untuk aplikasi bio-Si cair per bibit adalah: (i) blanko (tanpa pupuk), (ii) 5 g NPK 15-15-15, (iii) 5 g NPK  15-15-15 + 109cfu mikroorganisme pelarut silika (MPS), (iv-viii) 5 g NPK 15-15-15 + 25 ml; 50 ml; 75 ml; dan 100 mLbio-Si, dan 50 ml Na2SiO3. Pengamatan yang dilakukan meliputi analisis prolin, aktivitas nitrat reduktase (ANR), kandungan klorofil, serta morfologi stomata pada daun bibit kelapa sawit. Berdasarkan data fisiologi yang diperoleh dari kegiatan penelitian ini, aplikasi bio-Si dapat meningkatkan ketahanan bibit kelapa sawit terhadap cekaman kekeringan. Perlakuan bio-Si memberikan respon positif terhadap konsentrasi prolin,aktivitas nitrat reduktase (ANR), kandungan klorofil, serta morfologi stomata.Dosis 5 g NPK 15-15-15 + 7,5 g bio-Si padat dan 5 g NPK 15-15-15 + 75 mLbio-Si cair dapat direkomendasikan untuk meningkatkan ketahanan bibit kelapa sawit terhadap cekaman kekeringan.  [Kata kunci: bio-Si, klorofil, aktivitas nitrat reduktase, mikroorganisme pelarut silika].

scholarly journals Effect of Pgrs and Nutrients on Growth, Nitrate Reductase Activity, Soluble Protein, Proline Content and Yield in Bhendi Hybrid Under Salinity

2017 ◽  
Vol 5 (1) ◽  
pp. 81-87
Author(s):  
Vishnuveni Murugan ◽  
Sivakumar Rathinavelu ◽  
Nandhitha Krishnadevaraj ◽  
Chandrasekaran Perumal
Keyword(s):  
Salicylic Acid ◽  
Nitrate Reductase ◽  
Plant Growth ◽  
Soluble Protein ◽  
Nitrate Reductase Activity ◽  
Foliar Application ◽  
Reductase Activity ◽  
Foliar Spray ◽  
Proline Content ◽  
Saline Condition

An experiment was conducted to study the effect of PGRs { gibberellic acid (10 ppm), brassinolide (0.5 ppm), salicylic acid (100 ppm), ascorbic acid (100 ppm), benzyl amino purine (5 ppm)} and nutrients { K2SO4 (0.5%) + FeSO4 (0.5%) + Borax (0.3%) mixture and 19:19:19 (1%) mixture} on growth, NR enzyme activity, proline, soluble protein content and yield of bhendi hybrid (COBh H 1) under saline condition. The treatments were given at 25 and 45 DAS as foliar spray. The results showed that, nitrate reductase activity, soluble protein and plant growth were reduced under saline condition where as proline content was increased compared to absolute control. Foliar application of PGRs and nutrients enhanced the NR activity, proline, soluble protein and plant growth under saline condition. Among the PGRs and nutrients, foliar application of salicylic acid (100 ppm) and brassinolide (0.5 ppm) showed the better performance to mitigate the effect of salinity.

scholarly journals Physiological screening of ruderal weed biomonitors of atmospheric nitrogen deposition

2021 ◽  
Vol 1 (1) ◽  
Author(s):  
D. Nayeli Martínez ◽  
Erick De la Barrera
Keyword(s):  
Nitrate Reductase ◽  
Nitrogen Deposition ◽  
Chlorophyll Content ◽  
Biomass Production ◽  
Nitrate Reductase Activity ◽  
Photosynthetic Efficiency ◽  
Reductase Activity ◽  
Atmospheric Nitrogen Deposition ◽  
Atmospheric Nitrogen ◽  
Bidens Pilosa

Background: Plants take up various species of reactive nitrogen and their different physiological responses to the increase of nitrogen availability can be useful in biomonitoring. Questions: Does atmospheric nitrogen deposition affect the physiology of ruderal weeds? Which species are most responsive to the nitrogen deposition? Studied species: Eleven ruderal weeds. Study site and dates: Morelia, Michoacán, Mexico. 2019. Methods: Under scenarios of 10, 20, 40 and 80 kg N ha-1year-1, we quantified plant responses of biomass production, nitrate reductase activity, chlorophyll content, photosynthetic efficiency, δ15N, nitrogen and carbon content. Results: Total biomass production increased with the rate of nitrogen deposition for Bidens pilosa, Chloris gayana,Lepidium virginicum, and Pennisetum setaceum, as chlorophyll content in B. pilosa, C. gayana, and L. virginicum. In turn, the below- to above-ground biomass ratio decreased for B. pilosa and C. gayana, as photosynthetic efficiency in C. gayana, L. virginicum, and Chloris pycnothrix. Nitrate reductase activity was only affected in L. virginicumm, C. gayana, and T. officinale.    With the exception of C. pycnothrix, the nitrogen content increased, while the carbon augmented in C. gayana, C. pycnothrix, and P. setaceum. The C/N ratio was reduced in B. pilosa, C. gayana, Chloris virgata, P. setaceum, and T. officinale. The δ15N was increased in B. pilosa, C. gayana, C. virgata and P. setaceum. Conclusions: Bidens pilosa, C. gayana, L. virginicum, and P. setaceum were the species with more affected variables to nitrogen deposition, which could be useful in the biomonitoring.

Mitigating effect of foliar applied ascorbic acid on morpho-physiological, biochemical changes and yield attributes induced by salt stress in Vigna radiate

2017 ◽  
Vol 37 (2) ◽  
Author(s):  
Jagdish Kumar Nagda ◽  
Nishant A. Bhanu ◽  
Nishant A. Bhanu ◽  
Deepmala Katiyar ◽  
Akhouri Hemantaranjan ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Superoxide Dismutase ◽  
Salt Stress ◽  
Nitrate Reductase ◽  
Chlorophyll Content ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Combined Treatment ◽  
Threshold Level ◽  
Yield Attributes

The present investigation was carried out to examine the role of exogenously applied ascorbic acid which mitigates the deleterious effects of salt stress in mungbean (Vigna radiata L.) genotype HUM-1. Plants grown under induced salinity stress at 150 mM NaCl were treated with different concentration of ascorbic acid, i.e., 0.5 mM, 1.0 mM and 2.0 mM. To study the effects of treatments of salt stress on chlorophyll content, proline content, nitrate reductase activity, superoxide dismutase activity and yield attributes data were recorded at 20, 40, 60 day after sowing. Nitrate reductase activity and chlorophyll content with 1.0 mM ascorbic acid under salinity (150 mM NaCl) while the activities of superoxide dismutase get reduced up to 43.71% at 40 days after sowing. In plant treated with combined treatment of 150 mM NaCl and 1.0 mM foliar applied ascorbic acid caused a decline in the level of proline, which was 3.38 mg, 3.35 mg and 6.30 mg at 20, 40 and 60 days after sowing. The threshold level of ascorbic acid was 150 mM NaCl along with 1.0 mM ascorbic acid, that improved the yield attributes under salinity. Ascorbic acid inhibits the adverse effect of NaCl for growth and development of plants. So ascorbic acid may be a promising treatment to ameliorate the deleterious effects of salt stress in crops.

scholarly journals Understanding the Role of the Antioxidant System and the Tetrapyrrole Cycle in Iron Deficiency Chlorosis

Plants ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 348 ◽  
Author(s):  
Carla S. Santos ◽  
Rengin Ozgur ◽  
Baris Uzilday ◽  
Ismail Turkan ◽  
Mariana Roriz ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Iron Deficiency ◽  
Antioxidant System ◽  
Reductase Activity ◽  
Activity Levels ◽  
Alkaline Soils ◽  
Ferric Reductase ◽  
Ros Scavenging ◽  
Iron Deficiency Chlorosis ◽  
No Formation

Iron deficiency chlorosis (IDC) is an abiotic stress often experienced by soybean, owing to the low solubility of iron in alkaline soils. Here, soybean lines with contrasting Fe efficiencies were analyzed to test the hypothesis that the Fe efficiency trait is linked to antioxidative stress signaling via proper management of tissue Fe accumulation and transport, which in turn influences the regulation of heme and non heme containing enzymes involved in Fe uptake and ROS scavenging. Inefficient plants displayed higher oxidative stress and lower ferric reductase activity, whereas root and leaf catalase activity were nine-fold and three-fold higher, respectively. Efficient plants do not activate their antioxidant system because there is no formation of ROS under iron deficiency; while inefficient plants are not able to deal with ROS produced under iron deficiency because ascorbate peroxidase and superoxide dismutase are not activated because of the lack of iron as a cofactor, and of heme as a constituent of those enzymes. Superoxide dismutase and peroxidase isoenzymatic regulation may play a determinant role: 10 superoxide dismutase isoenzymes were observed in both cultivars, but iron superoxide dismutase activity was only detected in efficient plants; 15 peroxidase isoenzymes were observed in the roots and trifoliate leaves of efficient and inefficient cultivars and peroxidase activity levels were only increased in roots of efficient plants.

scholarly journals Responses of tomato (Solanum lycopersicum L.) plants to iron deficiency in the root zone

2019 ◽  
Vol 31 (1) ◽  
pp. 223-234
Author(s):  
María Rocío Jiménez ◽  
Laura Casanova ◽  
Teresa Saavedra ◽  
Florinda Gama ◽  
María Paz Suárez ◽  
...  
Keyword(s):  
Iron Deficiency ◽  
Chlorophyll Content ◽  
Solanum Lycopersicum ◽  
Root Zone ◽  
Reductase Activity ◽  
Chlorophyll Concentration ◽  
Root Tip ◽  
Tomato Plants ◽  
Young Leaves ◽  
Root Swelling

AbstractIron deficiency induces a yellowing in the aerial part of plants, known as iron chlorosis, and reduces the growth, yield, and quality of the fruits. Understanding plant response to iron deficiency is essential for agronomic management. This study decoded the temporal response of tomato plants (Solanum lycopersicum L.) to iron deficiency by quantifying different vegetative parameters. Subapical root swelling in the first 2.0 mm and several shoot and root growth parameters were measured in plants grown in a nutrient solution with and without Fe, on different dates designated as days after transplantation (DAT). Correlations between the total chlorophyll concentration in young leaves and 22 morphological and physiological parameters were also calculated. The plants grown in the absence of Fe had a higher number of secondary roots at 3 DAT, compared to control plants. On the same date, subapical root swelling was also observed, particularly at 1.5 and 2.0 mm from the root tip. Those plants also had a lower chlorophyll content in young leaves and a higher ferric-chelate reductase activity (FCR; EC 1.16.1.17) in the roots. At 9 DAT, the overall vegetative performance (plant height, fresh weight of stems and leaves) was negatively affected. At the end of the experiment (14 DAT), significant correlations were found between chlorophyll and the studied parameters. In conclusion, tomato plants experienced a cascade of responses to Fe deficiency throughout nine days: firstly, root lateralization increased; later, root swelling was observed, and a decrease in leaf chlorophyll content was registered associated with an increase in root FCR. At the end, the biomass of tomato plants decreased.

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