scholarly journals Comparative assessment of sanitary and epidemic importance of indicator coliform indices of drinking water quality

2019 ◽  
Vol 95 (6) ◽  
pp. 582-588 ◽  
Author(s):  
Yu. A. Rakhmanin ◽  
Lyudmila V. Ivanova ◽  
T. Z. Artemova ◽  
E. K. Gipp ◽  
A. V. Zagaynova ◽  
...  
Keyword(s):  
Water Quality ◽  
Pathogenic Bacteria ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Test Volume ◽  
Intestinal Infections ◽  
Scientific Substantiation ◽  
Ferment Lactose ◽  
The One ◽  
The Cost

The used methodology of the scientific substantiation of indicators is in the establishment of the conformity of laws of vital activity of indicator and pathogenic microorganisms in the real conditions of the action of the complex of factors, including disinfecting agents. In the one water sample simultaneously there were determined both the general indicator (GICB), thermotolerant (TTCB), glucose positive (GPCB) coliform bacteria, E.coli. On the base of long-term research in the various regions of the Russian Federation, as well with bearing in mind the analysis of domestic and foreign data, comparing the water quality and the incidence of intestinal infections in population it is recommended to use the index of determination of the total number glucose positive coliform bacteria (GPCB), which brings together a much broader range of bacteria of the Enterobacteriaceae family in comparison with total coliform bacteria (TCB) and thermotolerant coliform bacteria (TTCB) and warrants the absence in the test volume of water as an indicator lactose positive (E.coli, TCB, TTCB) and pathogens (salmonella) and potentially pathogenic bacteria which do not ferment lactose. Proposed index of GPCB is shown to allow to assess epidemiological risks not only more accurate, but also more efficiently without increasing the cost performance of bacteriological research.

scholarly journals Analysis of Water Quality Based on Coliform Bacteria Parameters in Springs of Sumberbening Village, Malang District

2021 ◽  
Vol 5 (1) ◽  
pp. 17-23
Author(s):  
Dyah Kumalasari ◽  
Nirmala Fitria Firdhausi ◽  
Ita Ainun Jariyah
Keyword(s):  
Water Quality ◽  
Pathogenic Bacteria ◽  
Quality Standard ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Physical Parameters ◽  
Clean Water ◽  
A Value ◽  
Quality Of Water ◽  
Sanitation And Hygiene

Clean water and free from pathogenic bacteria is the most important requirement to meet sanitation and hygiene needs and meet drinking needs. Most of the 3 springs in Sumberbening Village, Malang Regency, are mostly used by local communities and animals around protected forests. This research objective was to determine the quality of water based on the number of Coliform bacteria contamination and physical parameters to support the testing of 3 springs in Sumberbening Village, Malang Regency. This research was conducted in Kedung Trubus, Sendang Ngentup, and Sumber Trubus using descriptive exploratory methods. The results showed that all the springs were contaminated with Coliform bacteria with the highest total Coliform value in the Sendang Ngentup spring of 58.3 colonies / 100ml, while the highest fecal Coliform was found in Kedung Trubus spring 10.7 colonies / 100ml. The utilization of water in 3 springs to meet the drinking needs to be processed first. The physical parameters of Odor and Color indicate that all springs are odorless and colorless. The physical parameters of TDS show that only the spring water does not meet the quality standards of drinking water PERMENKES No. 492 of 2010 with a value exceeding 500mg / l. All springs based on the physical parameters of TDS meet the clean water requirements based on the clean water quality standard PERMENKES No. 32 of 2017 with a value <1000mg /l.

MICROBES PARAMETERS OF WATER QUALITY FOR AQUACULTURE ON PARI ISLAND WATERS

2013 ◽  
Vol 5 (1) ◽  
Author(s):  
Lies Indah Sutiknowati
Keyword(s):  
Pathogenic Bacteria ◽  
Heterotrophic Bacteria ◽  
Total Coliform ◽  
Total Cell ◽  
Epifluorescence Microscopy ◽  
Coliform Bacteria ◽  
Total Density ◽  
Shellfish Aquaculture ◽  
Microbiological Parameters ◽  
Phosphate Nitrate

The objective of this research was to evaluate waters quality in Pari island waters for aquaculture purpose based on bacteriological information conducted in Mei and September 2011. Microbiological parameters analyzed were total density of bacteria for coliforms, E.coli, pathogenic, heterotrophic, halotoleran, phosphate-nitrate-ammonia breaker, and total cells. Method to analyze coliform bacteria was filtration, identification of pathogenic bacteria using biochemical test, density analises for heterotrophic bacteria, analises for phosphate-nitrate-ammonia breaker bacteria using pour plate, and total cell using Acridine Orange Epifluorescence Microscopy. Results showed that the abundance of total coliform cell was about 1000-7000 colony forming unit (cfu)/100 ml. The abundance of heterotrophic, halotolerant, phosphate-nitrate-ammonia bacteria in seawater was (3.6-4.3)x105 cfu/ml, (1.1-1.3)x105 cfu/ml, (0.5-3.44)x103cfu/ml; and (3.6-6.7)x105 cfu/ml, (1.6-2.7)x105 cfu/ml, (0.6-5.22)x103 cfu/ml in sediment, respectively. The total cell of bacteria was (0.05-2.1)x107cells/ml. The dog-conch (Strombus turturella) and blood-clamps (Anadara granosa) can survive in Pari Island and there was a significant increase in sea grass litter with growth average of 0.67 mm/day and 0.90 mm/day. During snails and clamps growth, there were found several genus of pathogenic bacteria such as Salmonella, Vibrio, Aeromonas, Pseudomonas, Citrobacter, Proteus, Shigella, Hafnia, and Yersinia. The results showed that Pari island waters was suitable for developing shellfish aquaculture dog conch and blood clamps. Keywords: bacteria, parameter, shellfish, aquaculture.

scholarly journals Improvement of waste water quality by application of mixed algal inocula

2018 ◽  
Vol 53 (1) ◽  
pp. 77-82
Author(s):  
MA Riaz ◽  
B Ijaz ◽  
A Riaz ◽  
M Amjad
Keyword(s):  
Waste Water ◽  
Experimental Treatment ◽  
Total Coliform ◽  
Post Treatment ◽  
Coliform Bacteria ◽  
Physiochemical Parameters ◽  
Pollutant Concentrations ◽  
Pre Treatment ◽  
Wastewater Samples ◽  
The One

Present study aim to investigate application of microalgae for improvement of physiochemical parameters of waste water and improvement of treatment process through reducing the pollutant concentrations including coliform bacteria through their ability to uptake nutrients. Aquatic cultures were conducted in container with dimensions of 1.5×3sq ft. and wastewater samples 5 Litre samples were collected in plastic cans from Sukhnar drain and algal samples were collected from Bhaini Sigyain Village near Ravi Siphon, Lahore. The one experimental treatment lasted for 10 days to make 50% 5 liter water added concentration and 200g of inocula of algae were grown in wastewater container for experiments to optimize at room temperature as local outdoor cultures conditions for wastewater treatment. Total experiment repeated thrice and completed in 30 days. Results show that treatments improved the physiochemical parameter after week. Turbidity (NTU), total suspended solids (mg/L), total dissolved solids (mg/L), nitrate (mg/L), phosphate (mg/L) decrease (P < 0.05) after treatment. However, pH not significantly decreases (P≥ 0.05) pre-treatment values 6.56±0.05 (mg/L) and post-treatment 6.53± 0.057(mg/L). Maximum total coliform disinfection (P < 0.05) was indicated with 200 g of algae was pre-treatment 1602± 6.8 (MPN) and post treatment 1447±39.5 (MPN) observed.Bangladesh J. Sci. Ind. Res.53(1), 77-82, 2018

scholarly journals Analysis of Water Quality and River Waters Microbology for Manifestation of Food Safety

2019 ◽  
Vol 1 (1) ◽  
Author(s):  
Rizky Muliani Dwi Ujianti ◽  
Althesa Androva
Keyword(s):  
Water Quality ◽  
Food Security ◽  
Quality Management ◽  
Water Quality Management ◽  
Quality Standard ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Domestic Waste ◽  
Total Coliform Bacteria

 Abstract. Banjir Kanal Barat is a river in the Garang watershed, Semarang City, Central Java, Indonesia. Its function is as a source of water for the community. The level of pollution in this river is already high. The purpose of this study is to provide advice to governments, communities and related stakeholders to realize integrated river management, and fisheries-based food security is achieved. This research method is: analyzing the water quality of the Banjir Kanal Barat river, and analyzing the amount of faecal and total coliform bacteria content in the Banjir Kanal Barat river, and analyzing how to overcome the decline in the quality of waters of the Banjir Kanal Barat river due to faecal and total coliform bacteria pollution. The results showed that the water quality at the research location was still in the quality standard. The content of coliform dan faecal bacteria at the study site exceeds the quality standard, this is due to the influence of domestic waste from households. The thing that needs to be done is counseling the existence of a clean and healthy life, especially for people who are still throwing domestic waste into the river. The existence of water purification equipment is also very necessary to overcome this problem. Water quality management can be done with policy analysis. Regulations related to water quality management can be analyzed and then given solutions and recommendations related to these rules so that policies can be taken that are sustainable, integrated, and coordinated between various parties in managing river water quality and food security. Keywords: food security, water quality, river, faecal coliform, total coliform

scholarly journals ANALISIS KELIMPAHAN TOTAL BAKTERI COLIFORM DI PERAIRAN MUARA SUNGAI SAYUNG, MOROSARI, DEMAK (Analysis of Total Abundance of Coliform Bacteria at the Sayung River Estuary, Morosari, Demak)

2018 ◽  
Vol 14 (1) ◽  
pp. 30
Author(s):  
Lani Febriana Safitri ◽  
Niniek Widyorini ◽  
Oktavianto Eko Jati
Keyword(s):  
Water Quality ◽  
Organic Matter ◽  
Dissolved Oxygen ◽  
Random Sampling ◽  
River Estuary ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Total Abundance ◽  
Average Abundance ◽  
Total Coliform Bacteria

Perairan Muara Sungai Sayung terletak di wilayah Kabupaten Demak sebelah barat. Perairan ini telah dipengaruhi oleh berbagai aktivitas manusia yang menyebabkan terjadinya penurunan kualitas perairan. Bakteri Coliform merupakan bakteri gram negatif yang berbentuk batang dan tidak membentuk spora. Bakteri ini merupakan bakteri indikator terjadinya pencemaran perairan maupun indikator keberadaan bakteri patogen lainnya. Perhitungan total bakteri Coliform perlu dilakukan untuk mengetahui adanya pencemaran limbah organik yang terjadi di Perairan Muara Sungai Sayung, Morosari, Demak. Tujuan dari penelitian ini adalah untuk mengetahui kelimpahan total bakteri Coliform, dan status perairan di Perairan Muara Sungai Sayung, Morosari, Demak. Penelitian ini juga bertujuan untuk mengetahui hubungan dari jumlah total bakteri Coliform dengan bahan organik terlarut dan oksigen terlarut (DO). Pelaksanaan penelitian ini dilakukan pada bulan Mei 2018. Metode yang digunakan dalam penelitian ini adalah metode survei dan teknik pengambilan sampel menggunakan metode random sampling yang dilakukan pada lima stasiun. Hasil penelitian menunjukkan bahwa kelimpahan rata-rata total bakteri Coliform yang diperoleh berkisar antara 4 x 103 – 550 x 10 3/100ml. Kelimpahan rata-rata total bakteri Coliform yang diperoleh pada setiap stasiun menunjukkan bahwa kandungan total bakteri Coliform telah melebihi baku mutu air menurut Keputusan Menteri Lingkungan Hidup Nomor 51 Tahun 2004. Keberadaan total bakteri Coliform memiliki hubungan yang lemah dengan kandungan bahan organik terlarut, sedangkan keberadaan total bakteri Coliform memiliki hubungan yang cukup dengan oksigen terlarut (DO) di perairan Muara Sungai Sayung Morosari, Demak. Sayung River Estuary is located in the western part of Demak Regency. This estuary has been affected by various human activities which have led to a decline in water quality. Coliform bacteria are a gram-negative bacteria that are rod-shaped and do not form spores. This bacterium is an indicator bacteria of water pollution and an indicator of the presence of other pathogenic bacteria. Total Coliform bacteria calculation needs to be carrid out to find out if there any contamination of organic waste in Sayung River Estuary, Morosari, Demak.. The purpose of this research was to determine total abundance of Coliform bacteria, water quality in Sayung River Estuary, Morosari, Demak and relationship between the total abundance of Coliform bacteria with dissolved organic matter and dissolved oxygen (DO). This research was carried out in May 2018. The method that used in this research were survey method and the sampling technique was random sampling method in five stations. The results showed that the average abundance of total Coliform bacteria obtained ranged between 4 x 103 - 550 x 103 / 100ml. Average abundance of total Coliform bacteria obtained at each station shows that the total content of Coliform bacteria has exceeded the water quality standard according to Minister of Environment Decree number 51 of 2004. The total presence of Coliform bacteria has a weak relationship with the content of dissolved organic matter, while the total number of Coliform bacteria have sufficient relationship with dissolved oxygen (DO) in the Sayung River Estuary,Morosari, Demak.

scholarly journals Enumeration of Total and Faecal Coliform Bacteria in Drinking Water of Khairpur City

1970 ◽  
Vol 24 (2) ◽  
pp. 163-165 ◽  
Author(s):  
Abdul Hussain Shar ◽  
Yasmeen F Kazi ◽  
Miandad Zardari ◽  
Irshad Hussain Soomro
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Membrane Filtration ◽  
Total Coliform ◽  
Faecal Coliform ◽  
World Health ◽  
Source Distribution ◽  
Coliform Bacteria ◽  
Residual Chlorine ◽  
Health Organization

Total coliform (TC) and faecal coliform (FC) bacteria were analyzed in drinking water of Khairpur city. Ninty samples were collected from main reservoir (source), distribution line and consumer taps. pH and residual chlorine of water samples were also determined. For bacteriological analysis inductively membrane filtration (MF) method was used for total coliform (TC) as well as faecal (FC) coliform bacteria. All samples were found contaminated with total coliform (TC) and faecal coliform (FC) and the counts were higher than the maximum microbial contaminant level (MMCL) established by World Health Organization (WHO). It was observed that pH was within the limits of WHO standard (6.5-8.5). The residual chlorine was not detected in any sample of drinking water. Bacteriologically the water quality of the drinking water is unsatisfactory.Keywords: Coliform, Escherichia coli, Water quality, Contamination, SanitationDOI: http://dx.doi.org/10.3329/bjm.v24i2.1266

scholarly journals ANALISIS TOTAL BAKTERI COLIFORM DI SUNGAI BANJIR KANAL BARAT DAN SILANDAK, SEMARANG Analysis of Total Coliform Bacteria in Banjir Kanal Barat and Silandak Rivers, Semarang

2020 ◽  
Vol 8 (4) ◽  
pp. 309-315
Author(s):  
Dilia Puspita Asih ◽  
Churun Ain ◽  
Niniek Widyorini
Keyword(s):  
Water Quality ◽  
Organic Matter ◽  
Dissolved Oxygen ◽  
Pearson Correlation ◽  
Oxygen Demand ◽  
Total Coliform ◽  
Biological Oxygen Demand ◽  
Coliform Bacteria ◽  
Survey Method ◽  
The Relationship

Sungai Banjir Kanal Barat dan Silandak merupakan sungai besar yang berada di Kota Semarang. Terdapat berbagai aktivitas di sepanjang aliran sungai tersebut, diantaranya aktivitas domestik dan industri dimana buangan limbah masuk ke dalam badan sungai sehingga menyebabkan penurunan kualitas perairan. Salah satu mikroorganisme yang terkandung dalam limbah domestik yang berperan sebagai indikator pencemaran yaitu bakteri coliform sehingga perlu dilakukan perhitungan total coliform guna mengetahui adanya pencemaran di Sungai Banjir Kanal Barat dan Silandak. Tujuan dari penelitian ini adalah untuk mengetahui kelimpahan total coliform, status mutu air berdasarkan PP Nomor 82 Tahun 2001 kelas II dan hubungan antara total coliform dengan bahan organik dan BOD (Biological Oxygen Demand) di Sungai Banjir Kanal Barat dan Silandak. Metode yang digunakan dalam penelitian ini yaitu metode survei. Pengambilan sampel terdiri dari 2 stasiun (A dan B) yang masing-masing terdapat 5 titik dan 2 kali pengulangan (P1 dan P2). Variabel utama yang dianalisis yaitu total coliform, bahan organik dan BOD, variabel pendukung yang diukur yaitu temperatur, salinitas, DO (Dissolved Oxygen ) dan pH. Berdasarkan hasil penelitian, jumlah total coliform pada stasiun A berkisar antara 240-16000 MPN/100 mldan 23-5400 MPN/100 ml pada stasiun B. Nilai total coliform pada titik A2 dan B2 pada P1 serta A5 P2telah melebihi bakumutu. Hubungan antara total coliform dengan bahan organik yaitu sangat lemah (nilaiPearson Correlation = 0,15). Hubungan antara total coliform denganBOD yaitu lemah (nilaiPearson Correlation= 0,378). Banjir Kanal Barat and Silandak Rivers are large rivers in Semarang. There are various activities along the river flow, including domestic activities and industries where waste effluents enter the river bodies causing a decrease in water quality. One of the microorganisms contained in domestic waste that as an indicator of pollution is coliform bacteria, so it is necessary to calculate the total coliform in order to find out the pollution in the Banjir Kanal Barat and Silandak Rivers. This study aims to determine abundance of total Coliform, water quality status based on PP No. 82 of 2001 class II and relationship between the total coliform with organic matter and BOD (Biological Oxygen Demand) in the Banjir Kanal Barat and Silandak Rivers. The method used in this study is survey method. Sampling consisted of 2 stations (A and B), each of which had 5  points and 2 repetitions (P1 and P2). The main variables analyzed were total coliform, organic matter and BOD, the supporting variable analiyzed were temperature, salinity, DO (Dissolved Oxygen ) and pH. Based on the results of the study, the total number of coliform at station A ranged from 240-16000 MPN/100 ml and 23-5400MPN/100 ml at station B. The total value of coliform at points A2 and B2 at P1 and A5 P2 has exceeded the standard. The relationship between total coliform and organic matter is very weak (Pearson Correlation value = 0.15). The relationship between total coliform and BOD is weak (Pearson Correlation value = 0.378). 

scholarly journals The rationale for introducing the indices «Generalized coliform bacteria» and «Escherichia coli» into the scheme of sanitary and microbiological control of water quality as indices of fecal contamination

2021 ◽  
Vol 99 (12) ◽  
pp. 1353-1359
Author(s):  
Angelika V. Zagainova ◽  
Galina M. Trukhina ◽  
Yury A. Rakhmanin ◽  
Tamara Z. Artemova ◽  
Marina A. Sukhina
Keyword(s):  
Escherichia Coli ◽  
Water Quality ◽  
Drinking Water ◽  
Fecal Contamination ◽  
Coliform Bacteria ◽  
E Coli ◽  
Intestinal Infections ◽  
Safety Of Drinking Water ◽  
Microbiological Indicators

Introduction. The increasing bacterial contamination of water bodies requires an increase in water quality control’s reliability to ensure epidemic safety against waterborne infections. Therefore, researchers in both Russia and Europe came to the conclusion that it is necessary to search for indicator microorganisms that can more accurately suggest the presence of pathogens. microorganisms in water than traditional indicators. The aim of the study was to justify the introduction of indicator indices of fecal contamination “generalized coliform bacteria” and Escherichia coli to assess the safety of drinking water Material and methods. The article provides an analysis of domestic and international regulatory documents and literary materials regulating the quality of drinking water in terms of sanitary and microbiological indicators and assessment criteria. The results of many years of experimental and field research carried out by research organizations and practical organizations of the Federal Service for Supervision of Consumer Rights Protection and Human Welfare are presented. Results. On the territory of Russia, “general coliform bacteria,” is one of the indicator indices chosen according to the fermentation of lactose, determine the safety of drinking water. The water does not take into account pathogenic bacteria (Salmonella, Shigella) and a number of lactose-negative opportunistic bacteria, causative agents of intestinal infections. The study of microorganisms isolated from the feces of patients confirms the frequency of occurrence of lactose-negative microorganisms to varying from 20 to 100% of strains. With an annual trend towards a decrease in the percentage of non-standard drinking water samples in terms of microbiological indices, general intestinal infections (GII) of unknown etiology increase, i.e. risk of GII. If the quality of drinking water does not correspond to thermotolerant coliform bacteria (TCB), 95% of samples contain E. coli. Therefore, the determination of E. coli more reliably indicates the intake of fresh fecal contamination and provides efficiency in taking measures to eliminate an unfavorable situation than TCB. Conclusion. Reasons are given for the introduction of more reliable microbiological indicators of water safety control, such as - “generalized coliform bacteria” with the preservation of the abbreviation GCB, combining both lactose-positive and lactose-negative bacteria, determined by the sign of glucose fermentation, negative oxidase test and negative stain according to Gram and E. coli as an indicator of recent faecal contamination, which will allow the assessment of water quality for a wide range of bacteria of the order Enterobacterials, corresponding to the modern taxonomy of Enterobacteriaceae NCBI, will ensure harmonization with international requirements and the safety of drinking water for the population.

scholarly journals Water Quality of Rooftop Rainwater Harvesting System (MyRAWAS)

2021 ◽  
Vol 880 (1) ◽  
pp. 012039
Author(s):  
N U M Nizam ◽  
M M Hanafiah ◽  
M B Mokhtar ◽  
N A Jalal
Keyword(s):  
Water Quality ◽  
Rainwater Harvesting ◽  
Oxygen Demand ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Temperature Conductivity ◽  
Average Value ◽  
Safe Level ◽  
Before And After

Abstract Prolonged drought, population growth and water demand for various purposes have increased the water scarcity issue. To overcome this issue, a rainwater harvesting system can be utilized as an alternative for clean water supply. A rainwater harvesting system is a method of collecting rainwater from man-made surfaces such as rooftops and constructed surfaces and can be used for various sectors including household, agricultural and commercial. This study was conducted to determine the quality of rainwater harvested collected directly from rooftop. The quality of the rooftop rainwater was taken in three consecutive months and the water quality for before and after treatment was measured and compared. Commercial activated carbon was used to treat the rainwater obtained from the rooftop. The water quality was compared with the Water Quality Index (WQI) and the National Water Quality Standards (NWQS). The parameters involved are pH, temperature, conductivity, dissolved oxygen (DO), total suspended solids (TSS), ammoniacal nitrogen (NH3-N), biochemical oxygen demand (BOD), chemical oxygen demand (COD),E.coli and total coliform bacteria. The results showed that the total value of WQI before and after treatment was 86.3 ± 8.963 and 87.6±2.081, respectively. Positive correlations were found for parameter NH3-N, COD and pH, while paired T-test showed a significant in the COD and the presence of bacteria. Total Coliform is still at a safe level by NWQS with the average value and the standard deviation for before and after treatment were 38.11 ± 13.960 cfu/ml and 10.33 ± 6.671 cfu/ml, respectively.

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