scholarly journals Growth and survival of giant gourami juvenile immersed in freshwater containing growth hormone

2013 ◽  
Vol 11 (2) ◽  
pp. 162
Author(s):  
Pustika Ratnawati ◽  
. Alimuddin ◽  
Harton Arfah ◽  
Agus Oman Sudrajat
Keyword(s):  
Growth Hormone ◽  
Growth Rate ◽  
Body Weight ◽  
Immersion Time ◽  
Egg Yolk ◽  
Recombinant Growth Hormone ◽  
Fish Hatchery ◽  
Growth And Survival ◽  
Controlled Conditions

<p>Growth of giant gourami is relatively slow. Administration of recombinant growth hormone (rGH) by several<br />times immersion in water containing 0.9% NaCl for one hour can significantly increase the growth. In this<br />study, giant gourami juvenile were immersed in freshwater containing 120 mg/L rGH to determine the long<br />immersion time that provides higher growth and survival. Juvenile at seventh-day-old after egg yolk absorbed<br />was immersed for 0.5, 1.0, 2.0, and 3.0 hours in rGH solution. As controls, fish was immersed in freshwater<br />without rGH (control 1: C1), and water containing 0.9% NaCl, and 120 mg/L rGH (control 2: C2). Fish was<br />kept under controlled conditions in 20 L aquarium for three weeks, then in 50 L aquarium until at end of<br />experiment, and fed at satiation. The results showed that highest growth in body weight, survival, and biomass<br />(p&lt;0.05) were obtained in rGH immersion treatment for 0.5 hour. Biomass of fish immersed by rGH for 0.5<br />hour was about 32% higher compared to control C1, and 13% compared to C2. Survival of the 0.5 hour rGHimmersed<br />fish (100%) was similar to control C1 and C2. Thus, immersion of giant gourami juvenile in<br />freshwater containing rGH for 0.5 hour allowed highest growth rate, and this method is relatively easy to be<br />adopted by fish hatchery.</p><p>Keywords: immersion, hormone, growth, freshwater, giant gourami</p>

scholarly journals Effect of thyroxine and rGH hormone recombinant on growth and survival goldfish larvae, Carassius auratus (Linnaeus, 1758)

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
. Erlangga ◽  
. Zulfikar ◽  
. Hariyati
Keyword(s):  
Growth Hormone ◽  
Carassius Auratus ◽  
Absorption Rate ◽  
Egg Yolk ◽  
Fish Growth ◽  
P Value ◽  
Recombinant Growth Hormone ◽  
Growth And Survival ◽  
Yolk Absorption ◽  
Yolk Volume

Slow growth is obstacles to achieving the target of goldfish production (Carassius auratus auratus). The process of metabolism and growth in fish is influenced by hormonal factors. This research was conducted in February-March 2016 at Hatchery and Aquaculture Technology Laboratory Aquaculture Study Program of Faculty of Agriculture University of Malikussaleh. This study aims to examine the effect of thyroxine (T4) and hormone recombinant Growth Hormone (rGH) hormone by immersion method with different doses on growth and survival of goldfish larvae. The experimental method using a complete non factorial randomized design with four three replication treatments. The treatment is: A= control, B= T4 0.05 mgL-1 + rGH 5 mgL-1, C= T4 0.1 mgL-1 + rGH 10 mgL-1, D = T4 0.15 mgL-1 + rGH 15 mgL-1. Result of research with giving of recombination of hormone T4 and hormone rGH with different dose have an effect on to decrease of yolk volume, egg yolk absorption rate, growth and survival with p value <0,05 . The best treatment is in treatment D (T4 0, 15mgL-1 + rGH 15 mg L-1) with yolk volume and egg yolk absorption rate for 36 hours, growth weight: 3.09 mg, growth length: 1.75 mm, and 81% stability. The values of waters quality the study was 27-28 oC, pH 7.0-7.4, dissolved oxygen 5-6.1 ppm and ammonia 0.1073-0.1793 ppm, still support optimal fish growth for the goldfish. This research is expected to accelerate the growth process of the goldfish larvae with excellent syntax value.AbstrakPertumbuhan yang lambat merupakan kendala dalam mencapai target produksi ikan maskoki (Carassius auratus auratus). Proses metabolisme dan pertumbuhan pada ikan dipengaruhi oleh faktor hormonal. Penelitian ini dilaksa-nakan pada bulan Februari-Maret 2016 di Laboratorium Pembenihan dan Teknologi Budidaya, Perairan Program Studi Budidaya Perairan, Fakultas Pertanian, Universitas Malikussaleh. Penelitian ini bertujuan untuk menguji pe-ngaruh pemberian hormon tiroksin (T4) dan hormon rGH (recombinant Growth Hormone) melalui metode peren-daman dengan dosis yang berbeda terhadap pertumbuhan dan sintasan larva ikan maskoki. Metode penelitian ekspe-rimental dengan rancangan acak lengkap non faktorial dengan empat perlakuan tiga ulangan. Adapun perlakuannya adalah: A= Kontrol, B= T4 0,05 mgL-1+ rGH 5 mgL-1, C= T4 0,1 mgL-1+ rGH 10 mgL-1, D = T4 0,15 mgL-1+ rGH 15 mgL-1. Hasil penelitian dengan pemberian rekombinasi hormon T4 dan hormon rGH dengan dosis yang berbeda ber-pengaruh terhadap penurunan volume kuning telur, laju penyerapan kuning telur, pertumbuhan dan sintasan dengan nilai p< 0,05. Perlakuan terbaik terdapat pada perlakuan D (T4 0,15 mg L-1 + rGH 15 mgL-1) dengan hasil volume kuning telur dan laju penyerapan kuning telur selama 36 jam, pertumbuhan bobot : 3,09 mg, pertumbuhan panjang : 1,75 mm, dan sintasan : 81 %. Nilai kualitas air selama penelitian yaitu suhu 27-28 oC, pH 7,0-7,4, oksigen terlarut 5-6,1 mgL-1 dan amonia 0,1073-0,1793 mgL-1, masih mendukung pertumbuhan ikan yang optimal bagi ikan mas koki. Penelitian ini diharapkan mampu mempercepat proses pertumbuhan larva ikan mas koki dengan nilai sintasan yang sangat baik.

scholarly journals GROWTH PERFORMANCE AND HISTOLOGICAL APPEARANCE OF THE HUMPBACK GROUPER JUVENILE (CROMILEPTESALTIVELIS) AFTER TREATED WITH RECOMBINANT GROWTH HORMONE

2015 ◽  
Vol 2 (1) ◽  
pp. 191
Author(s):  
Suci Antoro ◽  
M Zairin Junior ◽  
A Alimuddin ◽  
Agus Suprayudi ◽  
Irvan Faizal
Keyword(s):  
Growth Hormone ◽  
Growth Rate ◽  
Growth Stimulation ◽  
Oral Route ◽  
High Price ◽  
Recombinant Growth Hormone ◽  
Commercial Diet ◽  
Long Time ◽  
Kidney Liver ◽  
Daily Application

Despite as high price consumption fish, humpback grouper grow out take very long time so its culture considered not efficient. Therefore to accelerate its growth rate and make grow out culture more efficient, recombinant Epinepheluslanceolatus growth hormone (rElGH) was applied by oral route. Daily application of rough rElGH at a dose of 5 mg/100 g commercial diet for 42 days resulted significance increase in growth rate compared to control groups. No specific histological damage on kidney, liver and spleen which was attributable to rElGH administration. These results strongly suggested that growth stimulation following oral administration was due to a specific action of rElGH and recombinant GH as mentioned above save for fish consumption. Keywords: growth, histology, humpback grouper, recombinant growth hormone 

scholarly journals Growth of green catfish seed fed on sludge worm and artificial feed combination

2014 ◽  
Vol 12 (1) ◽  
pp. 18
Author(s):  
Netti Aryani ◽  
Niken Ayu Pamungkas ◽  
. Adelina
Keyword(s):  
Growth Rate ◽  
Body Weight ◽  
Survival Rate ◽  
Artificial Diet ◽  
Daily Growth Rate ◽  
Daily Growth ◽  
Growth And Survival ◽  
Absolute Length ◽  
The Absolute ◽  
Mystus Nemurus

<p class="NoParagraphStyle" align="center"><strong>ABSTRACT</strong></p><p class="NoParagraphStyle" align="center"> </p><p class="NoParagraphStyle">This research aimed to obtain information of growth and survival of green catfish (<em>Mystus nemurus</em>) juvenile fed with sludge worm (<em>Tubifex</em> sp.; T) and artificial diet (PB) and added with the combination of 50% soybean pulp waste and 50% freshwater trash fish. Feeding was performed in several variation of time during 40 days of fish rearing. Average body length of juvenile was 12 mm and weight 2.8±0.0 mg, maintained at a density of 30 individuals/aquarium. The treatment in this research was T10PB30 (8–18 days old juvenile were fed with sludge worm and 18–48 days old fish were fed with artificial diet), T20PB20 (8–28 days old juvenile were fed with sludge worm and 28–48 days old were fed with artificial diet), T30PB10 (8–38 days old juvenile were fed with sludge worm and 38–48 days old were given artificial diet), PB40 (8–48 days old juvenile were fed artificial diet), T40 (8–48 days old juvenile were fed with sludge worm). The results indicated that the treatment of 40 days feeding with sludge worm provided the best growth and survival as daily growth rate of 16.4±28.0 g/day, the growth of the absolute length was 43.60±0.01 mm, the absolute body weight 2,047.2±35.0 mg and the survival rate was 96.44%. The best artificial feeding treatment was feeding with sludge worm for 30 days and with 10 days of artificial diet, results in daily growth rate of 4.53±0,25 mm/day, the growth of the absolute length 40.00±0.04 mm, the absolute body weight 1,447.2±15.0 mg, and the survival rate 94.44±2.60%.</p><p class="NoParagraphStyle" align="center"> </p><p class="NoParagraphStyle">Keywords: artificial feed, sludge worm, growth, green catfish</p><p class="NoParagraphStyle" align="center"> </p><p class="NoParagraphStyle" align="center"> </p><p class="NoParagraphStyle" align="center"><strong>ABSTRAK</strong></p><p class="NoParagraphStyle" align="center"> </p><p class="NoParagraphStyle">Penelitian ini bertujuan untuk mendapatkan informasi pertumbuhan dan sintasan benih ikan baung (<em>Mystus nemurus</em>) dengan pemberian cacing sutra (<em>Tubifex</em> sp.; T) dan pakan buatan (PB) kombinasi 50% limbah ampas tahu dan 50% ikan rucah air tawar. Pakan diberikan dengan variasi lama waktu berbeda selama pemeliharaan 40 hari. Rerata panjang awal benih adalah 12 mm dan bobot tubuh 2,8±0,0 mg, dipelihara dengan kepadatan 30 ekor/akuarium. Perlakuan dalam penelitian ini adalah T10PB30 (benih umur 8–18 hari diberi pakan cacing sutra dan umur 18–48 hari pakan buatan), T20PB20 (benih umur 8–28 hari diberi cacing sutra dan umur 28–48 pakan buatan), T30PB10 (benih umur 8–38 diberi pakan cacing sutra dan umur 38–48 diberi pakan buatan), PB40 (benih umur 8–48 hari diberi pakan buatan), T40 (benih umur 8–48 hari diberi pakan cacing sutra). Hasil penelitian ini menunjukkan bahwa perbedaan lama waktu pemberian cacing sutra dan pakan buatan yang terbaik adalah pemberian pakan cacing sutra selama 40 hari dengan laju pertumbuhan harian 16,4±28,0 g/hari, pertumbuhan panjang mutlak 43,60±0,01 mm, bobot mutlak 2.047,2±35,0 mg, dan sintasan 96,44%. Selanjutnya variasi lama waktu pemberian pakan buatan yang terbaik adalah pemberian cacing sutra selama 30 hari dan pakan buatan sepuluh hari dengan laju pertumbuhan harian sebesar 4,53±0,25 mm/hari, pertumbuhan panjang mutlak 40,00±0,04 mm, bobot mutlak 1.447,2±15,0 mg, dan sintasan 94,44±2,60%.</p><p class="NoParagraphStyle" align="center"> </p><p class="NoParagraphStyle">Kata kunci: pakan buatan, cacing sutra, pertumbuhan, ikan baung</p><p> </p>

scholarly journals Role of insulin-like growth factor-1 and growth hormone in growth inhibition induced by magnesium and zinc deficiencies

1991 ◽  
Vol 66 (3) ◽  
pp. 505-521 ◽  
Author(s):  
Inge Dørup ◽  
Allan Flyvbjerg ◽  
Maria E. Everts ◽  
Torben Clausen
Keyword(s):  
Growth Hormone ◽  
Growth Rate ◽  
Body Weight ◽  
Growth Inhibition ◽  
Zn Deficiency ◽  
Relative Pair ◽  
Mg Deficiency ◽  
And Zn Deficiency ◽  
Old Rats ◽  
Catch Up

Nutritional deficiencies of magnesium or zinc lead to a progressive and often marked growth retardation. We have evaluated the effect of Mg and Zn deficiency on growth, serum insulin-like growth factor-1 (s-IGF-1), growth hormone (s-GH) and insulin (s-insulin) in young rats. In 3-week-old rats maintained on Mg-deficient fodder for 12 d the weight gain was reduced by about 34%, compared with pair-fed controls. This was accompanied by a 44% reduction in s-IGF-1, while s-insulin showed no decrease. After 3 weeks on Mg-deficient fodder, growth had ceased while serum Mg (s-Mg) and s-IGF-1 were reduced by 76 and 60% respectively. Following repletion with Mg, s-Mg was completely normalized in 1 week, and s-IGF-1 reached control level after 2 weeks. Growth rate increased, but the rats had failed to catch up fully in weight after 3.5 weeks. Absolute and relative pair-feeding were compared during a Mg repletion experiment. Absolute pair-fed animals were given the same absolute amount of fodder as the Mg-deficient rats had consumed the day before. Relative pair-fed animals were given the same amount of fodder, on a body-weight basis, consumed in the Mg-deficient group the day before. In a repletion experiment the two methods did not differ significantly from each other with respect to body-weight, muscle weight, tibia length and s-IGF-1, although there was a tendency towards higher levels in the relative pair-fed group. The peak in s-GH after growth hormone-releasing factor 40 (GRF 40) was 336 (se 63) μg/l in 5-week-old rats that had been Mg depleted for 14 d, whereas age-matched control animals showed a peak of 363 (se 54) μg/l (not significant).In 3-week-old rats maintained on Zn-deficient fodder for 14 d weight gain was reduced by 83% compared with pair-fed controls. Serum Zn (s-Zn) and s-IGF-1 were reduced by 80 and 69% respectively, while s-insulin was reduced by 66%. The Zn-deficient animals showed a more pronounced growth inhibition than that seen during Mg deficiency and after 17 d on Zn-deficient fodder s-IGF-1 was reduced by 83%. Following repletion with Zn, s-Zn was normalized and s-IGF-1 had increased by 194% (P <0.05) after 3 d. s-IGF-1, however, was not normalized until after 2.5 weeks of repletion. Growth rate increased but the catch-up in weight was not complete during 6 weeks. The maximum increase in s-GH after GRF 40 was 774 (se 61) μg/l in control animals ν. 657 (se 90) μg/l in 6-week-old rats that had been Zn-depleted for 12 d (not significant). In conclusion, both Mg and Zn deficiency lead to growth inhibition that is accompanied by reduced circulating s-IGF-1, but unchanged s-GH response. Zn deficiency, but not Mg deficiency, caused a reduction in s-insulin. The reduction in s-IGF-1 could not be attributed to reduced energy intake, but seems to be a specific effect of nutritional deficiency of Mg or Zn. It is suggested that the growth retardation seen during these deficiency states may be mediated through reduced s-IGF-1 production.

scholarly journals PRODUCTION AND BIOACTIVITY POTENTIAL OF THREE RECOMBINANT GROWTH HORMONES OF FARMED FISH

2010 ◽  
Vol 5 (1) ◽  
pp. 11 ◽  
Author(s):  
Alimuddin Alimuddin ◽  
Indra Lesmana ◽  
Agus Oman Sudrajat ◽  
Odang Carman ◽  
Irvan Faizal
Keyword(s):  
Growth Hormone ◽  
Body Weight ◽  
Protein Expression ◽  
Common Carp ◽  
Nile Tilapia ◽  
Expression Vector ◽  
Expression Vectors ◽  
Farmed Fish ◽  
Recombinant Growth Hormone ◽  
E Coli

This study was aimed to produce recombinant growth hormone (rGH) from giant grouper (Epinephelus lanceolatus), giant gouramy (Osphronemus gouramy) and common carp (Cyprinus carpio) and compare their bioactivity potential by means of inducing the growth hormone of juvenile Nile tilapia (Oreochromis niloticus) as the model. DNA fragment encoding mature GH protein of giant grouper (El-mGH), giant gouramy (Og-mGH) and common carp (Cc-mGH) was amplified by PCR method. The purified PCR products were ligated to pCold-1 to generate pCold/El-mGH, pCold/OgmGH, and pCold/Cc-mGH protein expression vector, respectively. Each of the expression vectors was transformed into the Escherichia coli BL21. E. coli BL21 was cultured using 2xYT medium and protein production was induced by cold shock at 15±1oC for overnight. The inclusion bodies of E. coli transformants containing protein expression vector were isolated by sonication method, and rGH production was analyzed by SDS-PAGE. Juvenile of Nile tilapia of average body weight of 12.41±3.28 g was intramuscularly injected once a week for 4 weeks with 1 μg inclusion body containing rGH per gram fish body weight. The result showed that rGH in molecular weight of about 25 kDa was obtained. Fish injected with rGH of El-mGH, Cc-mGH and Og-mGH grew 20.94%, 18.09%, and 16.99% faster, respectively, compared with the control. This result indicated that the three rGH produced in E. coli possessed biological activity when tested on Nile tilapia and further research is needed to find its effect on the growth of other aquaculture fish species.

scholarly journals Comparison of Sulfate Metabolism in Costal Cartilage of Normal and 'Little' Mice

1983 ◽  
Vol 36 (3) ◽  
pp. 263 ◽  
Author(s):  
NeiI M McKern
Keyword(s):  
Growth Hormone ◽  
Growth Rate ◽  
Body Weight ◽  
Costal Cartilage ◽  
Cartilage Degradation ◽  
Skeletal Growth ◽  
Sulfate Uptake ◽  
Sulfate Metabolism ◽  
C 50

C57BL/6J and mutant 'little' (lit/lit) mice c. 50 days of age were injected with doses of [35S]sulfate proportional to their body weight. Despite the diminished growth rate of lit/lit mice compared with normal mice at this age, uptake of radioactivity per unit mass of cartilage was similar for both mouse types, confirming previous data. Additional experiments with these mice established that the similarity of sulfate uptake could not be accounted for by differences in the location of bound sulfate or (for females) by differences in cartilage cellularity. Investigation of sulfate loss by costal cartilage in vivo indicated that cartilage degradation occurred at a greater rate in lit/lit mice than in normally growing mice. These latter data suggest that growth hormone, which is lacking in lit/lit mice, may in part regulate skeletal growth (at least for female mice) by inhibiting degradation of cartilage.

scholarly journals Influence of the size and form of Artemia sp. nauplii on the growth and survival of Atlantic sturgeon (Acipenser oxyrinchus Mitchill) larvae

2021 ◽  
Vol 29 (2) ◽  
pp. 69-79
Author(s):  
Iwona Piotrowska ◽  
Bożena Szczepkowska ◽  
Michał Kozłowski
Keyword(s):  
Growth Rate ◽  
Body Weight ◽  
Survival Rate ◽  
Specific Growth Rate ◽  
Growth Rates ◽  
Specific Growth ◽  
Survival Rates ◽  
Atlantic Sturgeon ◽  
Growth And Survival ◽  
Acipenser Oxyrinchus

Abstract The aim of the study was to determine the influence of the size and form of Artemia sp. feed on the rearing indices of Atlantic sturgeon larvae. In the experiment, the nauplius sizes used were 480 µm (group AD), 430 µm (group AM), and 430 µm frozen nauplii (group AI). After three weeks of rearing, the sturgeon from group AD had the highest mean body weight of 0.131 g that was statistically significantly different from groups AM (0.071 g) and AI (0.033 g). The longest total length was noted in group AD (2.8 cm) and was statistically different from the others (P ≤ 0.05). Specific growth rate in groups AD and AM was 14.7 and 11.8% d−1, respectively, and these values differed statistically from those in group AI at 7.8% d−1. The highest survival rate was recorded in group AD at 60.6%, while the lowest was noted in group AI at 15.6%, and the differences between these two groups were statistically significant. The results of the experiment indicated that a feed of live 480 µm Artemia sp. nauplius significantly accelerated the growth rates and increased the survival rates of Atlantic sturgeon larvae.

Influence of bovine growth hormone on growth rate, appetite, and food conversion of yearling coho salmon (Oncorhynchus kisutch) fed two diets of different composition

1977 ◽  
Vol 55 (1) ◽  
pp. 74-83 ◽  
Author(s):  
J. R. Markert ◽  
David A. Higgs ◽  
Helen M. Dye ◽  
D. W. MacQuarrie
Keyword(s):  
Growth Hormone ◽  
Growth Rate ◽  
Body Weight ◽  
Coho Salmon ◽  
Lower Percentage ◽  
Bovine Growth Hormone ◽  
Growth Promoter ◽  
Ration Level ◽  
Diet Formulation ◽  
Treated Fish

Paired groups of yearling coho salmon at 10 °C and on a natural photoperiod were fed twice daily either Oregon moist pellets (OMP) or a diet with poultry offal (PO) as the main protein source. The fish were fed either 0.8% body weight per day (dry weight of food (g)/wet weight of fish (g)) or to satiation. Coho in one of the groups for each diet–ration combination received, by intramuscular injection, 10 μg (0.0092 IU) of bovine growth hormone (bGH) per gram body weight one time per week for 56 days. Those in the other group were not treated.Administration of bGH significantly enhanced growth and improved food and protein conversion. Ration level altered the effect of bGH on food and protein conversion as did diet formulation at satiation. The voluntary food intake of bGH-treated fish fed OMP was higher but not significantly different from that of the controls. Diet formulation differences did not significantly affect the growth rate of either bGH-treated or untreated coho at each ration level. Irrespective of the diet–ration combination, bGH administration lead to a significant decline in condition factor.At 56 days, bGH-treated fish had a significantly lower percentage of muscle protein and a significantly higher percentage of muscle water than untreated fish. At present, the use of bGH as a growth promoter for coho salmon is not economical.

Recombinant growth hormone in growing lambs: effects on growth, feed utilization, body and carcass characteristics and on wool growth

1989 ◽  
Vol 40 (1) ◽  
pp. 195 ◽  
Author(s):  
AS Zainur ◽  
R Tassell ◽  
RC Kellaway ◽  
WR Dodemaide
Keyword(s):  
Growth Hormone ◽  
Body Weight ◽  
Recombinant Dna ◽  
Plasma Concentrations ◽  
Recombinant Growth Hormone ◽  
Linear Change ◽  
Wool Growth ◽  
Quadratic Response ◽  
The Mean ◽  
Plasma Gh

Groups of six crossbred lambs, three wethers and three ewes, about 22 kg liveweight (LW), were injected subcutaneously with 0, 50, 150 and 250 pg (kg LW) d a y 1 of recombinant DNA-derived bovine growth hormone (rebGH) for 60 days. From blood sampled twice weekly 4.5 h after injection, the mean plasma concentrations of growth hormone (GH) (P<0.01) and insulin (P<0.05) were significantly increased. The mean concentrations of GH were 2.9, 7.1, 9.4 and 8.8 8g/l, while insulin concentrations were 24.2, 29.5, 46.8 and 68.2 mU/l for the respective treatments. Maximum concentrations of plasma GH were recorded about 3 h after injection. Rates of liveweight gain were 180, 217,238 and 261 g/day (P<0.001), fccd conversion ratios were 5.71,4.97,4.66 and 4.05 g feed/g gain ( P < 0.001), and warm carcase weights were 14.4, 16.4, 16.6 and 16.8 kg (P < 0.0 1) for the respective treatments. The linear change in warm carcase weights became a quadratic response (P<0.05) when adjusted to an empty body weight (EBW) of 28.8 kg. Wool growth was not affcctcd by treatments. Of the non-carcase components, major organs including the head (P<0.01), feet (P<0.05), heart (P<0.05), liver and gallbladder (P < 0.05), reticula-rumen (P < 0.05), omasum (P<0.05) and blood (P<0.01) showed linear increases in weight with increasing dosage of rebGH, but no significant differences when data were adjusted to a standard EBW. The chemical composition of the carcase showed that crude protein increased linearly (P<0.001) with increasing doses of rebGH. When adjusted to 28.8 kg empty body weight, there was 16.0�/o more carcase protein (P<0.01) with 250 than with 0 8g (kg LW)-1 day-1 of rebGH.

scholarly journals APLIKASI HORMON PERTUMBUHAN REKOMBINAN UNTUK MENINGKATKAN LAJU PERTUMBUHAN IKAN KAKAP (Lutjanus sp.) PADA KERAMBA JARING APUNG DI TELUK TALENGEN

2021 ◽  
Vol 7 (2) ◽  
pp. 14-21
Author(s):  
Aprelia Tomasoa ◽  
Walter Balansa ◽  
Krisan Salendeho
Keyword(s):  
Growth Hormone ◽  
Growth Rate ◽  
Stocking Density ◽  
Egg White ◽  
Control Treatment ◽  
Recombinant Growth Hormone ◽  
Cage System ◽  
Test Fish ◽  
Pellet Feed ◽  
Positive Effect

Budidaya ikan kakap sering terkendala dengan kurang tersedianya pakan rucah secara kontinyu dan berkelanjutan, karena pakan rucah tersedia secara musiman. Budidaya ikan kakap harus diselingi dengan pemberian pakan pelet, apalagi pakan pelet yang diperkaya dengan hormon pertumbuhan rekombinan dapat menjadi salah satu solusi. Mempercepat laju pertumbuhan ikan dapat dilakukan dengan pemberian hormon pertumbuhan rekombinan. Hormon tersebut dijadikan sebagai sumplemen pakan bagi ikan maupun udang. Tujuan penelitian ini adalah untuk meningkatkan laju pertumbuhan ikan kakap menggunakan hormon pertumbuhan rekombinan yang dibudidayakan dengan sistem keramba jaring apung di Teluk Talengen. Tahapan penelitian terdiri dari persiapan wadah dan ikan uji, pembuatan pakan dan pemeliharaan selama 30 hari. Perlakuan yang dilakukan adalah dosis hormon pertumbuhan rekombinan yang diaplikasikan ke pakan dan diberikan pada ikan kakap. Dosis yang diberikan adalah : 0 mg/kg, 2 mg/kg, 3 mg/kg, dengan tiga kali ulangan. Ikan kakap yang digunakan berukuran 3-5 cm dengan padat tebar 10 ekor per wadah. Dosis hormon pertumbuhan rekombinan akan dicoating menggunakan putih telur dan disemprot ke pakan. Pemberian pakan dilakukan secara at satiation dengan frekuensi pemberian sebanyak 2 kali pada pagi dan sore hari. Hasil penelitian memperlihatkan perlakuan terbaik yaitu dosis 3 mg/kg pakan meningkatkan bobot tubuh (8,8 gr), SGR (3,43%), EP (28,3%) dan SR (100%) dibandingkan perlakuan kontrol. Hal tersebut menunjukkan hormon pertumbuhan rekombinan memberi pengaruh positif meningkatkan laju pertumbuhan dan survival rate ikan kakap selama 30 hari pemeliharaan.   Snapper cultivation is often constrained by the lack of continuous and sustainable feed availability, because trash feed is readily available. The cultivation of snapper fish must be interspersed with providing pellet feed, moreover pellet feed enriched with recombinant growth hormone can be a solution. Accelerating the growth rate of fish can be done by offering recombinant growth hormone. This hormone is used as a supplement to feed for fish and shrimp. The aim of this study was to increase the growth rate of snapper using recombinant growth hormone cultivated with floating net cage system in Talengen Bay. Stages of taking care of research from containers and test fish, making feed and maintaining it for 30 days. The treatment is a recombinant growth hormone which is applied to feed and given to snapper. The doses given were: 0 mg/ kg, 2 mg/kg, 3 mg/kg, with three replications. The snapper used is 3-5 cm in size with a stocking density of 10 fish per container. The dose of recombinant growth hormone will be coated using egg white and sprayed into the feed. Feeding is done occasionally with a frequency of offering 2 times in the morning and evening. The results of the best treatment treatment, namely the dose of 3 mg / kg of feed increased body weight (8.8 gr), SGR (3.43%), EP (28.3%) and SR (100%) compared to control treatment. This shows that the growth of recombinant growth hormone has a positive effect on increasing the growth rate and survival of snapper for 30 days of rearing.

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