Comparaison Quantitative Et Qualitative Des Huiles Essentielles De Rosmarinus Officinalis Obtenues Par Différentes Méthodes

Rosmarinus officinalis is among the most abundant medicinal and aromatic plants in Morocco. There are several factors that influence the yield of quality and quantity of essential oils of this plant. This work focuses on the study, characterization and comparison of the chemical composition of essential oils of Rosmarinus officinalis. The raw material studied is originally from the central part of northern Morocco (Skoura M'Daz region, Eastern Middle Atlas). The essential oils of rosemary were obtained by three different extraction methods : DA: Artisanal distillation, the essential oils were obtained directly from small cooperatives using a very traditional (nonindustrial) method and based on the technique of Steam distillation ; DI: Industrial distillation which is also based on Steam distillation; DC: hydrodistillation by clevenger apparatus Laboratory. The physicochemical characterization of essential oils focuses on the search for the yield of % essential oil with regard to the dry rosemary biomass, density, refractive index and rotatory power. The chemical analyzes were carried out by gas chromatography (GC), they identified 11 components representing more than 99.64% of the essential oil and indicate that the chemotype is 1.8 cineole and is varied according to the method (DI: 48.83%, DA: 41.28% and DC: 51.77%). In conclusion we have shown a quantitative and qualitative loss of essential oils due to the extraction method of essential oils, which has direct repercussions on the economy of rural Morocco and consequently on their sustainable development.

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Chemical Composition, Antioxidant, and Antimicrobial Activities of Rosmarinus officinalis Essential Oil From Moroccan Middle Atlas

Phytothérapie ◽

10.3166/phyto-2019-0128 ◽

2019 ◽

Vol 18 (3-4) ◽

pp. 162-168 ◽

Cited By ~ 1

Author(s):

T. El Kamli ◽

M. El Hamdani ◽

N. Eloutassi ◽

F. Errachidi ◽

R. Chabir ◽

...

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Steam Distillation ◽

Antibacterial Activities ◽

Microtiter Plate ◽

Extraction Methods ◽

Antimicrobial Activities ◽

Rosmarinus Officinalis ◽

Gas Chromatography Mass Spectrometry ◽

Rosmarinus Officinalis L

This study was designed to evaluate antioxidant and antibacterial activities of essential oils from Rosmarinus officinalis obtained by three different extraction methods: DA: Artisanal distillation; the essential oils were obtained directly from small cooperatives using a very traditional (nonindustrial) method and based on the technique of Steam distillation; DI: Industrial distillation which is also based on steam distillation; and DC: hydrodistillation through Clevenger apparatus laboratory. The chemical analyses were carried out with gas chromatography/mass spectrometry (GC/MS); they identified 16 components representing more than 99.89% of the essential oil and indicate that the chemotype is 1,8-Cineole and varies according to the method used (DI: 49.09%, DA: 42.12%, and DC: 53.21%). The antioxidant activity was evaluated by the β-carotene bleaching test measuring percent inhibition of peroxidation in linoleic acid system. The disc diffusion and modified resazurin microtiter-plate assays were used, respectively, to evaluate the inhibition zones (IZ) and minimum inhibitory concentration (MIC) of Rosmarinus officinalis essential oil. In general, Rosmarinus officinalis L. essential oil showed a lower antioxidant and antimicrobial activity than 1,8-Cineole the major component of the essential oil.

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Chemical composition of Rosmarinus officinalis (Lamiaceae) essential oils and evaluation of their toxicity against Bruchus rufimanus (Coleoptera: Chrysomelidae: Bruchinae) in Morocco

International Journal of Tropical Insect Science ◽

10.1017/s1742758418000012 ◽

2018 ◽

Vol 38 (03) ◽

pp. 192-204 ◽

Cited By ~ 2

Author(s):

Karima Hannour ◽

Ahmed Boughdad ◽

Abdelwahed Maataoui ◽

Aziz Bouchelta

Keyword(s):

Essential Oils ◽

Health Risks ◽

Steam Distillation ◽

Storage Conditions ◽

Rosmarinus Officinalis ◽

Gas Chromatography Mass Spectrometry ◽

Control Methods ◽

Control Groups ◽

Lethal Time ◽

Middle Atlas

AbstractControl methods used to limit field losses caused by Bruchus rufimanus Boheman, 1833 consist of synthetic insecticides that pose health risks to farmers, consumers and the environment. In an attempt to find safer alternatives, we screened essential oils from the leaves of Rosmarinus officinalis collected in the Middle Atlas and Loukkos regions of Morocco in the laboratory as natural fumigants against B. rufimanus. These essential oils were extracted by steam distillation using a Clevenger distiller, characterised chemically by coupled gas chromatography–mass spectrometry (GC/MS) and tested at five concentrations (0, 10, 20, 40, 80 µl/l air). The essential oils of R. officinalis mainly comprised oxygenated monoterpenes (Middle Atlas: 79.4%; Loukkos: 48.78%) and terpenes (Middle Atlas: 14.71%; Loukkos: 32.33%). The lethal concentrations (LC50) of Middle Atlas and Loukkos essential oils against male beetles ranged from, respectively, 46.53 to 1.19 µl/l air and 58.85 to 11.57 µl/l air. Similarly, doses of R. officinalis essential oils from Middle Atlas and Loukkos lethal to females, ranged from 44.6 to 2.08 µl/l air and from 53.00 to 5.38 µl/l air, respectively. Additionally, the lethal time (LT50 and LT99) of exposed adults ranged from 1–8 and from 2–13 days for different concentrations of R. officinalis essential oils from Middle Atlas and Loukkos, respectively. With no mortality recorded in the control groups, these findings demonstrate the fumigant potential of these oils against this bruchid under the storage conditions.

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Solvent-Free Microwave Extraction of Thymus mastichina Essential Oil: Influence on Their Chemical Composition and on the Antioxidant and Antimicrobial Activities

Pharmaceuticals ◽

10.3390/ph14080709 ◽

2021 ◽

Vol 14 (8) ◽

pp. 709

Author(s):

André R. T. S. Araujo ◽

Sandrine Périno ◽

Xavier Fernandez ◽

Cassandra Cunha ◽

Márcio Rodrigues ◽

...

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Large Scale ◽

Steam Distillation ◽

Extraction Methods ◽

Antimicrobial Activities ◽

Solvent Free ◽

Microwave Extraction ◽

Dry Distillation ◽

Antioxidant And Antimicrobial Activities

Solvent-free microwave extraction (SFME) is a combination of microwave heating and dry distillation performed at atmospheric pressure without the addition of water or organic solvents that has been proposed as a green method for the extraction of essential oils from aromatic and medicinal herbs. In this work, SFME and the conventional techniques of steam distillation (SD) and hydrodistillation (HD) were compared with respect to the extraction and antioxidant and antimicrobial activities of Thymus mastichina essential oil. The main constituent of essential oils obtained using different methods was 1,8-cineole (eucalyptol). The results showed that the essential oils extracted by means of SFME in 30 min were quantitatively (yield) and qualitatively (aromatic profile) similar to those obtained using conventional HD over 120 min. In addition, SFME generates less waste and less solvent, consumes less energy, and provides a higher yield for a shorter extraction time, which is advantageous for the extraction of the T. mastichina essential oil compared to SD. The antioxidant and antimicrobial activities of the T. mastichina essential oil obtained from either SFME or conventional extraction methods (SD or HD) showed a similar pattern. Large-scale experiments using this SFME procedure showed a potential industrial application.

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Essential oils of Cordia species, compounds and applications: a systematic review

Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas ◽

10.37360/blacpma.22.21.2.10 ◽

2022 ◽

Vol 21 (2) ◽

pp. 156-175

Author(s):

Kellen Cruvinel Rodrigues Andrade ◽

◽

Diegue Henrique Nascimiento Martins ◽

Diogo de Amorim Barros ◽

Paula Monteiro de Souza ◽

...

Keyword(s):

Systematic Review ◽

Essential Oil ◽

Essential Oils ◽

Steam Distillation ◽

Antioxidant Activities ◽

Selection Process ◽

Extraction Methods ◽

Oil Extraction ◽

Biological Application ◽

Inclusion Criteria

The purpose of this systematic review was to identify the available literature on the essential oil from species of genus Cordia. This study followed the Preferred Reporting Items for Systematic Reviews. The search was conducted on four databases: LILACS, PubMed, Science Direct, and Scopus until June 5th, 2020, with no time or language restrictions. Sixty out of the 1,333 initially gathered studies fit the inclusion criteria after the selection process. Nine species of Cordiawere reported in the selected studies, out of which 79% of the evaluated studies reported essential oil from Cordia curassavica. The essential oil extraction methods identified were hydrodistillation and steam distillation. As for biological application, antimicrobial, anti-inflammatory, larvicidal and antioxidant activities were the most reported. The main compounds reported for essential oil were β-caryophyllene, α-humulene, α-pinene, bicyclogermacrene, and sabinene. The information reported in this systematic review can contribute scientifically to the recognition of the importance of the genus Cordia.

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The Effect of Temperature on the Essential Oil Components of Salvia potentillifolia Obtained by Various Methods

Natural Product Communications ◽

10.1177/1934578x0900400730 ◽

2009 ◽

Vol 4 (7) ◽

pp. 1934578X0900400 ◽

Cited By ~ 1

Author(s):

Mehmet Öztürk ◽

Gülsen Tel ◽

Mehmet Emin Duru ◽

Mansur Harmandar ◽

Gülaçtı Topçu

Keyword(s):

High Temperature ◽

Essential Oil ◽

Essential Oils ◽

Volatile Compounds ◽

Steam Distillation ◽

Extraction Methods ◽

Effect Of Temperature ◽

Chemical Compositions ◽

Percentage Concentration ◽

Oil Components

The chemical compositions of the essential oils of Salvia potentillifolia (Lamiaceae) were analyzed by GC and GCMS. The oils were obtained by four different methods (hydrodistillation, steam distillation, and two extraction methods) to investigate the effect of temperature on the volatile compounds. In total, 121 components were detected in the various oils. The major compounds characterized were α-pinene (30.2, 31.2, 10.6, and 14.8 %) and β-pinene (15.0, 14.6, 7.6, and 11.4%), respectively. Surprisingly, the percentage concentrations of α-pinene and β-pinene in the hydrodistilled and steam distilled oils were high, even though prepared at high temperature, whereas the contents of both compounds were lower in the essential oils obtained by the extraction methods prepared without added heat. The percentage concentration of 8,13-epoxy-labda-14-en-2-one, a diterpenoid, in the extracted essential oil was 22 times higher than in the hydrodistilled and steam distilled essential oils. However, the concentrations of cis-p-menth-2-en-1-ol was four times, and β-bourbonene and β-caryophyllene two times superior than their percentage concentrations in the hydrodistilled and steam distilled essential oils.

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Extraction of Essential Oils of Rosmarinus officinalis L. by Two Different Methods: Hydrodistillation and Microwave Assisted Hydrodistillation

The Scientific World JOURNAL ◽

10.1155/2019/3659432 ◽

2019 ◽

Vol 2019 ◽

pp. 1-6 ◽

Cited By ~ 11

Author(s):

Majda Elyemni ◽

Bouchra Louaste ◽

Imane Nechad ◽

Taha Elkamli ◽

Abdelhak Bouia ◽

...

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Steam Distillation ◽

Azeotropic Distillation ◽

Rosmarinus Officinalis ◽

Extraction Time ◽

Microwave Assisted ◽

Rosmarinus Officinalis L

The extraction of essential oils is generally carried out by two main techniques: azeotropic distillation (hydrodistillation, hydrodiffusion, and steam distillation) and extraction with solvents. However, these traditional methods are a bit expensive, especially since they are extremely energy and solvent consuming. This work consists in studying two methods of extraction of the essential oils of Rosmarinus officinalis L.: microwave assisted hydrodistillation (MAH) and Clevenger hydrodistillation (CH). Several parameters have been studied: the extraction time, the yield, and the chemical composition of the essential oils as well as the efficiency and cost of each procedure. The results obtained revealed that microwave-assisted hydrodistillation makes it possible to minimize the extraction time of the essential oils in comparison with conventional hydrodistillation. Thus, the same yield of essential oils is obtained for 20 minutes only with MAH while it takes 180 minutes with CH. In addition, the quality of the essential oil is improved thanks to a 1.14% increase in oxygenates. In conclusion, the MAH method offers significant advantages over conventional hydrodistillation and can therefore replace it on a pilot and industrial scale.

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Hydrolates: By-products of essential oil distillation: Chemical composition, biological activity and potential uses

Advanced technologies ◽

10.5937/savteh2002054a ◽

2020 ◽

Vol 9 (2) ◽

pp. 54-70

Author(s):

Milica Aćimović ◽

Vele Tešević ◽

Katarina Smiljanić ◽

Mirjana Cvetković ◽

Jovana Stanković ◽

...

Keyword(s):

Biological Activity ◽

Essential Oil ◽

Essential Oils ◽

Food Industry ◽

Fruits And Vegetables ◽

Steam Distillation ◽

Raw Material ◽

Chemical Compositions ◽

Organoleptic Properties ◽

By Products

Hydrolates, also referred to as hydrosols, floral or distillate waters, as well as aromatic waters, are produced in the same isolation process with essential oils by steam distillation. A small amount of essential oil constituents is dissolved in hydrolates providing specific organoleptic properties and flavor, as well as biological activity which makes them useful as raw material in many industries. Their popularity is still on the rise, especially in aromatherapy. The objective in this review is to analyze the chemical compositions of hydrolates and their corresponding essential oils, as well as biological activity of hydrolates (antimicrobial, antioxidant and antiinflamatory) and potential uses, not only in food industry for flavoring, and preservation of fresh-cut fruits and vegetables, but also as functional (soft) drinks. However, hydrolates can be used in aromatherapy and cosmetics, as well as in organic agriculture and aquaculture.

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Chemical Composition and Antibacterial Activity of Essential Oils of Three Medicinal Plants from Algerian Semi-Arid Climatic Zone

Phytothérapie ◽

10.3166/phyto-2019-0150 ◽

2018 ◽

Vol 16 (S1) ◽

pp. S155-S163 ◽

Cited By ~ 2

Author(s):

S. Mehalaine ◽

O. Belfadel ◽

T. Menasria ◽

A. Messaili

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Rosmarinus Officinalis ◽

Salvia Officinalis ◽

Thymus Algeriensis

The present study was carried out to determine, for the first time, the chemical composition and antibacterial activity of essential oils derived from the aerial parts of three aromatic plants Thymus algeriensis Boiss & Reut, Rosmarinus officinalis L., and Salvia officinalis L. growing under semiarid conditions. The essential oils were chemically analyzed and identified by gas chromatography (GC) and GC/ mass spectrometry (GC/MS) and their antimicrobial activity was individually evaluated against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa using both agar disk diffusion and agar dilution methods. The major constituents of Thymus algeriensis essential oil were identified as camphor (13.62%), 1,8-cineol (6.00%), borneol (5.74%), viridiflorol (4.00%), and linalool (3.93%). For Rosmarinus officinalis essential oil, 48 compounds were characterized, of which the main constituents were camphor (17.09%), Z-β-ocimene (10.88%), isoborneol (9.68%), α-bisabolol (7.89%), and borneol (5.11%). While, Salvia officinalis essential oil was characterized by β-thujone (16.44%), followed by viridiflorol (10.93%), camphor (8.99%), 1,8-cineol (8.11%), trans-caryophyllene (5.85%), and α-humulene (4.69%) as the major components. Notably, results from antibacterial screening indicated that Thymus algeriensis and Salvia officinalis essential oils exhibited a strong inhibitory effect against both Escherichia coli and Staphylococcus aureus compared to Rosmarinus officinalis essential oil. Further, less activity was recorded against Pseudomonas aeruginosa for the three tested essential oils.

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Determination of Essential Oil Bioactive Components and Rosmarinic Acid of Salvia officinalis Cultivated under Different Intra-row Spacing

Notulae Scientia Biologicae ◽

10.15835/nsb529046 ◽

2013 ◽

Vol 5 (2) ◽

pp. 198-203 ◽

Cited By ~ 3

Author(s):

Mohammad ABU DARWISH ◽

Ezz Al-Dein AL-RAMAMNEH ◽

Ivan SALAMON ◽

Ziad ABU-DIEYEH ◽

Mohamed AL NAWAISEH ◽

...

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Rosmarinic Acid ◽

Plant Density ◽

Salvia Officinalis ◽

Raw Material ◽

Row Spacing ◽

Human Disorders ◽

Tea Production ◽

First Time

Salvia officinalis, known also as sage, is a medicinal plant belonging to the Lamiaceae family that spreads all over the word in several countries. The demand for the raw material and extracts of this plant is increasing due to its numerous applications in pharmacy, food and herbal tea production. The present study investigated for the first time the effect of 15, 30 and 45 cm intra-row spacing (plant density) on the main constituents of sage essential oils and rosmarinic acid content. The highest content of essential oils (2.7%) and rosmarinic acid (2.0%) were obtained in plants grown using 15 cm planting space. Likewise, close spacing resulted also in a substantial content of 1,8-cineole (47-50%, GC/FID; 55-60%, GC/MS). This work indicated that 1,8-cineole chemotype was a dominant character of cultivated S. officinalis in south of Jordan. In general, the percent of α-thujone in essential oil was not affected by intra-row spacing. However, the percent of β-thujone decreased from (2-3%, GC/MS) in plants grown using 15 cm intra-row spacing to (1-2%, GC/MS) in plants grown using 30 and 45 cm intra-row spacing. The highest content of α-and β-pinene was recorded in plants grown using 45 cm planting space (8-10%, GC/FID; 5-6% GC/MS). Based on GC/MS, camphor compound was enriched (9-10%) in sage plants grown under 15 cm spacing and greater than in plants grown under 30 (6-7%) or 45 cm (5-6%) spacing. The results make the potential use of sage extracts in the treatment of some human disorders or illness an area of further research.

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Compositional Analysis and Aroma Evaluation of Feijoa Essential Oils from New Zealand Grown Cultivars

Molecules ◽

10.3390/molecules24112053 ◽

2019 ◽

Vol 24 (11) ◽

pp. 2053 ◽

Cited By ~ 2

Author(s):

Yaoyao Peng ◽

Karen Suzanne Bishop ◽

Siew Young Quek

Keyword(s):

New Zealand ◽

Essential Oil ◽

Essential Oils ◽

Extraction Method ◽

Compositional Analysis ◽

Extraction Methods ◽

Essential Oil Composition ◽

Oil Composition ◽

Active Compounds ◽

Initial Comparison

Feijoa is an aromatic fruit and the essential oil from feijoa peel could be a valuable by-product in the juicing industry. An initial comparison of the essential oil extraction methods, steam-distillation and hydro-distillation, was conducted. The volatile compounds in the essential oils from four feijoa cultivars were identified and semi-quantified by GC-MS and the aroma active compounds in each essential oil were characterized using SPME-GC-O-MS. Hydro-distillation, with a material to water ratio of 1:4 and an extraction time of 90 min, was the optimized extraction method for feijoa essential oil. The Wiki Tu cultivar produced the highest essential oil yield among the four selected cultivars. A total of 160 compounds were detected, among which 90 compounds were reported for the first time in feijoa essential oils. Terpenes and esters were dominant compounds in feijoa essential oil composition and were also major contributors to feijoa essential oil aroma. Key aroma active compounds in feijoa essential oils were α-terpineol, ethyl benzoate, (Z)-3-hexenyl hexanoate, linalool, (E)-geraniol, 2-undecanone, 3-octanone, α-cubebene, and germacrene D. This is the first report on the optimization of the extraction method and the establishment of the aroma profile of feijoa essential oils, with a comparison of four New Zealand grown cultivars.

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