scholarly journals Etude Phytochimique Des Écorces De Racines Et Des Feuilles De Securidaca longipedunculata (Fresen), Polygalaceae Au Mali

2021 ◽  
Vol 17 (29) ◽  
pp. 145
Author(s):  
Dembele Daouda L ◽  
Haidara Mahamane ◽  
Denou Adama ◽  
Sanogo Rokia
Keyword(s):  
Free Radical ◽  
Chemical Constituents ◽  
Extraction Yield ◽  
Root Bark ◽  
Hydroalcoholic Extract ◽  
Light Yellow ◽  
African Traditional Medicine ◽  
Couche Mince ◽  
Utilisations Traditionnelles ◽  
Layer Chromatography

Securidaca longipedunculata est une plante largement utilisée en Médecine Traditionnelle Africaine. L’objectif de ce travail est de caractériser les constituants chimiques et anti-radicalaires des feuilles et des écorces de racines de la plante. Les échantillons ont été récoltés et contrôlés, des extraits ont été préparés et les rendements déterminés, les constituants ont été caractérisés par des réactions colorées et de précipitation et par la chromatographie sur couche mince. Les caractères macroscopiques des organes sont entre autres : lisse, épaisse et jaune clair, tortueuse, rugueuse avec une odeur caractéristique pour l’écorce et la racine ; alternes, entières, simples, oblongues-elliptiques, sommet arrondi avec un pétiole mince pour les feuilles. La poudre des échantillons est de couleur blanche sale, d’odeur forte repoussante et de saveur piquante pour les écorces de racines ; couleur verdâtre, odeur faible non repoussante et de saveur légèrement piquante pour les feuilles. Le meilleur rendement de l’extraction est 33,24% pour le décocté 20% des écorces de racines et 36,37% pour l’extrait hydroalcoolique de feuilles. Les constituants chimiques communs aux deux organes sont les composés triterpéniques et les coumarines ; plus particulièrement les flavonoïdes, tanins et les alcaloïdes sont les constituants majoritaires des feuilles. En plus les mucilages, oses et holosides sont modérément présents dans les deux organes. La richesse des échantillons des deux organes en ses constituants peut justifier certaines utilisations traditionnelles de la plante. Securidaca longipedunculata is a plant widely used in African Traditional Medicine. The objective of this work was to characterize the chemical constituents of the leaves and root bark of the plant. Samples were collected and checked, extracts were prepared and the yield were determined. The chemical and anti-free radical constituents were characterized by color and precipitation reactions and by thin layer chromatography. The macroscopic characters of the organs are, among others: smooth, thick and light yellow, twisted, rough with a characteristic odor to the root bark; alternate, entire, simple, oblong-elliptical, rounded top and a thin petiole for the leaves. The powder of the samples is dirty white, with a strong repulsive odor and pungent flavor for the root bark; greenish color, faint non-repulsive odor and slightly pungent flavor for the leaves. The best extraction yield was 33.24% for the aqueous extract 20% of the root bark and 36.37% for the hydroalcoholic extract of the leaves. The chemical constituents common to both organs are triterpene compounds and coumarins; while flavonoids, tannins and alkaloids are the major constituents of the leaves. In addition, mucilages, oses and holosides are moderately present in both organs. Samples from both organs were found to be rich in anti-free radical constituents. The richness of organ samples in these constituents may justify certain traditional uses of the plant.

Extraction and Purification of Secondary Metabolites of Paris polyphylla (Smith) Using Column Chromatography.

2021 ◽  
Vol 8 (1) ◽  
pp. 11-22
Author(s):  
Arcadius Puwein ◽  
Shiny C. Thomas
Keyword(s):  
Column Chromatography ◽  
Chemical Constituents ◽  
Extraction Yield ◽  
Herbaceous Plant ◽  
Anticancer Activities ◽  
Extraction And Purification ◽  
Paris Polyphylla ◽  
Rf Values ◽  
Tlc Analysis ◽  
Layer Chromatography

Paris polyphylla Smith is an erect and herbaceous plant. It has rich chemical constituents such steroidal saponins, phytosterols, flavonoids, and alkaloids that possess antimicrobial and anticancer activities. In the current investigation, we examined the effect of different solvents in the extraction yield and further purification via column chromatography. The ground powdered rhizomes of P. polyphylla was extracted with 100% hexane, 100% ethyl acetate, 70% ethanol, and 70% methanol. The extracts were filtered, evaporated using a rotary evaporator, concentrated, and measured. Subsequently, the solvent with high extraction yield was further purified using column chromatography. Ethanol produced the highest extraction yield as compared to the other solvents. About 30 fractions were eluted which was pooled into four fractions based on the Rf values and bands observed through thin layer chromatography (TLC) analysis.

Fibrinolytic, Anti-Inflammatory and Cytotoxic Potentialities of Extracts and Chemical Constituents of Manglicolous Lichen, Graphis ajarekarii Patw. & C. R. Kulk

2020 ◽  
Vol 10 (1) ◽  
pp. 87-93 ◽  
Author(s):  
Vinay Bharadwaj Tatipamula ◽  
Girija Sastry Vedula
Keyword(s):  
Protein Denaturation ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Fibrin Clot ◽  
Hydroalcoholic Extract ◽  
Paw Oedema ◽  
Anti Inflammatory ◽  
Rat Paw ◽  
Rat Paw Oedema

Background: Lichens which are betide to mangroves are termed as Manglicolous Lichens (ML). As these ML are habituated under stress conditions, they are screened for unique metabolites and biological activities. Objective: The study aimed to establish the chemical and biological profile of ML, Graphis ajarekarii. Methods: The Ethyl Acetate Extract of G. ajarekarii (EAE) was subjected to chromatographic techniques and the obtained isolates were characterized by spectroscopic analysis. The hydroalcoholic extract of G. ajarekarii (AE), EAE, isolates and Hydroalcoholic Extract of host (HE) were evaluated for fibrinolytic (fibrin clot method), in vitro (protein denaturation method) and in vivo (formalin-induced rat paw oedema assay), anti-inflammatory and cytotoxicity (MTT assay) activities. Results: Chemical investigation of the EAE led to the isolation of two known compounds namely atranorin (1) and ribenone (2), which were confirmed by spectral data. The AE and EAE gradually lysed the fibrin clot with 94.54 and 65.07%, respectively, at 24 h. The AE inhibited protein denaturation of about 88.06%, while the standard (Indomethacin) with 93.62%. Similarly, the in vivo antiinflammatory analysis of AE (200 mg/mL) showed potent reduction of rat paw oedema than the standard, whereas EAE and 1 depicted moderate depletion. In addition, the AE revealed prominence inhibition on MCF-7, DU145 and K-562 with IC50 values of 69.5, 42.5 and 38 µg/mL, respectively, whereas the HE exhibited mild inhibitory profile against fibrin clot, inflammation and cancer. Conclusion: From the results, it can be concluded that the G. ajarekarii has an aptitude to act against coagulation, inflammation and cancer cells.

Chemical Composition of Aegle marmelos: A Review

2021 ◽  
pp. 280-290
Author(s):  
Yogita Chowdhary
Keyword(s):  
Therapeutic Potential ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Biologically Active ◽  
Fixed Dose ◽  
Root Bark ◽  
Preclinical Studies ◽  
Aegle Marmelos ◽  
Crude Extracts ◽  
Anti Inflammatory

Aegle marmelos (Bilva) is being used in Ayurveda for the treatment of several inflammatory disorders. The plant is a member of a fixed dose combination of Dashamoola in Ayurveda. However, the usage of roots/root bark or stems is associated with sustainability concerns. Bael (Aegle marmelos (L.) Corr.) is an important medicinal plant of India. Leaves, fruits, stem and roots of A. marmelos have been used in ethno medicine to exploit its' medicinal properties including astringent, antidiarrheal antidysenteric, demulcent, antipyretic and anti-inflammatory activities. Compounds purified from bael have been proven to be biologically active against several major diseases including cancer, diabetes and cardiovascular diseases. Preclinical studies indicate the therapeutic potential of crude extracts of A. marmelos in the treatment of many microbial diseases, diabetes and gastric ulcer. This review covers the biological activities of some isolated chemical constituents of A. marmelos and preclinical studies on some crude extracts and pure compounds to explore novel bioactive compounds for therapeutic application. Aegle marmelos (L.) is a seasonal fruit that contains significant amounts of bioactives like, phenolic acids (gallic acids, 2,3-dihydroxy benzoic acid, chlorogenic acid, p-coumaric acid, vanillic acid), flavonoid (rutin), organic acids (oxalic acid, tartaric acid, malic acid, lactic acid, acetic acid, citric acid, propionic acid, succinic acid, fumaric acid), vitamin C, vitamin B group (thiamine, niacin, pyridoxine, pantothenic acid, biotin, cobalamins, riboflavin), tocopherols (α-tocopherol, β-tocopherol, γ-tocopherol, δ-tocopherol), carotenes (α-carotene, β-carotene, γ-carotene, δ-carotene) and also rich in essential minerals (potassium, calcium, phosphorus, sodium, iron, copper, manganese). Hence the use of aegle plays important role as anti-inflammatory.

Chemical constituents from the root bark of Cynanchum auriculatum

2018 ◽  
Vol 81 ◽  
pp. 30-32 ◽  
Author(s):  
Xin-Jie Wang ◽  
Xu-Hui Lv ◽  
Zhen-Lin Li ◽  
Shi-Hui Qian ◽  
She-Ban Pu
Keyword(s):  
Chemical Constituents ◽  
Root Bark ◽  
Cynanchum Auriculatum

scholarly journals High Performance Thin Layer Chromatography Fingerprinting Analysis of Piper betle L. Leaves

2021 ◽  
pp. 8-15
Author(s):  
Ramdas N. Kale ◽  
Ravindra Y. Patil
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Methanolic Extract ◽  
Chemical Constituents ◽  
Piper Betle ◽  
Soxhlet Apparatus ◽  
Rf Values ◽  
Pharmacologically Active ◽  
Layer Chromatography

Introduction: Many modern medicines used today based on plants and plant products. Piper betle is generally known as the betle vine, it is an important medicinal and recreational plant. High performance thin layer chromatography (HPTLC) is an advanced powerful analytical method with more separation power, high performance and superior reproducibility than classic thin layer chromatography (TLC). A chromatographic fingerprint of a plant extract is a chromatographic pattern of some common chemical constituents of pharmacologically active and/or chemical characteristics. Chromatographic fingerprints are useful in authentication and identification of plant. Objectives:  Objectives of present research was to establish HPTLC fingerprinting of methanolic extract of Piper betle L. leaves. Materials and Methods: Methanolic extract of Piper betle leaves was prepared using soxhlet apparatus. HPTLC studies were performed using a CAMAG HPTLC system equipped with automatic TLC sampler-4 (ATS 4), TLC scanner 4, and vision CATS 3.0 software. Results: The study revealed the presence of alkaloids with Rf value 0.65, flavonoids with Rf values 0.19, 0.29, 0.72, 0.95., and phenolic compound with Rf value 0.7. Conclusion: The HPTLC fingerprinting profile developed for the methanolic extract of Piper betle L. leaves will help in proper identification of the plant.Piper betle

scholarly journals Chemical constituents of Euphorbia tirucalli L.

2019 ◽  
Vol 2 (5) ◽  
pp. 76-82
Author(s):  
Dung Thi Kim Le ◽  
Hao Xuan Bui ◽  
Tuyet Thi Anh Nguyen ◽  
Tuyen Nguyen Kim Pham ◽  
Huy Thuc Duong
Keyword(s):  
Thin Layer ◽  
Gallic Acid ◽  
Spectroscopic Analysis ◽  
Chemical Constituents ◽  
Normal Phase ◽  
Chemical Structures ◽  
Euphorbia Tirucalli ◽  
Arjunolic Acid ◽  
First Time ◽  
Layer Chromatography

Euphorbia tirucalli has not been chemically studied much in Vietnam. This research described the isolation and elucidation of compounds isolated from the plant collected in Binh Thuan. Multiple chromatographic methods were applied, including normal phase silica gel column chromatography and thin-layer chromatography. Seven compounds were isolated and their chemical structures were elucidated by spectroscopic analysis as well as comparing their data with the ones in the literature. They are arjunolic acid (1), eriodictyol (2), quercitrin (3), afzelin (4), scopoletin (5), 3,3′,4- trimethylellagic acid (6), and gallic acid (7). Among them, compound 1 a major component was isolated for the first time in Euphorbia genus, while three compounds 2, 4, and 5 were isolated from this species for the first time.

scholarly journals SINTESIS CALKON ((E)-1,3-DI(NAPHTHALEN-2-YL)PROP-2-EN-1-ONE) DAN AKTIVITASNYA SEBAGAI ANTIOKSIDAN

2012 ◽  
Vol 3 (1) ◽  
pp. 7-14
Author(s):  
Rahmiwati Hilma ◽  
Jasril ◽  
Hilwan Yuda Teruna
Keyword(s):  
Free Radical ◽  
Melting Point ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Room Temperature ◽  
Intermediate Compound ◽  
Alkaline Condition ◽  
Point Measurement ◽  
Anti Inflammatory ◽  
Layer Chromatography

Study on chalcone calkon (E)-1,3-di(naphthalen-2-yl)prop-2-en-1-one synthesis have been carried out with stirrer methode. These compounds can be used as intermediate compound to synthesize others compounds which was reported having antimicrobial, anti-inflammatory, anti-depressant, anti-tumour. The of chalcones synthesis vatives were reported in acid and alkali condition. In this study, chalcone and its derivates were synthesized by using stirrer method in alkaline condition in room temperature. the compounds subjected to somes analyses including melting point measurement, thin layer chromatography and HPLC. Scavenging free radical by using DPPH methode showed Scavenging free radical with LC50 >80 μg/ml min potent activity while the ascorbat acid LC50 89,79 μg/ml.

scholarly journals An Antioxidant Potential, Quantum-Chemical and Molecular Docking Study of the Major Chemical Constituents Present in the Leaves of Curatella americana Linn

2018 ◽  
Vol 11 (3) ◽  
pp. 72 ◽  
Author(s):  
Mayara Teles Fujishima ◽  
Nayara Silva ◽  
Ryan Ramos ◽  
Elenilze Batista Ferreira ◽  
Kelton Santos ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Xanthine Oxidase ◽  
Quantum Chemical ◽  
Binding Free Energy ◽  
Chemical Constituents ◽  
Docking Study ◽  
Antioxidant Potential ◽  
Molecular Docking Study ◽  
Hydroalcoholic Extract ◽  
Curatella Americana

Reactive oxygen species (ROS) are continuously generated in the normal biological systems, primarily by enzymes as xanthine oxidase (XO). The inappropriate scavenging or inhibition of ROS has been considered to be linked with aging, inflammatory disorders, and chronic diseases. Therefore, many plants and their products have been investigated as natural antioxidants for their potential use in preventive medicine. The leaves and bark extracts of Curatella americana Linn. were described in scientific research as anti-inflammatory, vasodilator, anti-ulcerogenic, and hypolipidemic effects. So, the aim of this study was to evaluate the antioxidant potentials of leaf hydroalcoholic extract from C. americana (HECA) through the scavenging DPPH assay and their main chemical constituents, evaluated by the following quantum chemical approaches (DFT B3LYP/6-31G**): Maps of Molecular Electrostatic Potential (MEP), Frontier Orbital’s (HOMO and LUMO) followed by multivariate analysis and molecular docking simulations with the xanthine oxidase enzyme. The hydroalcoholic extract showed significant antioxidant activity by free radical scavenging probably due to the great presence of flavonoids, which were grouped in the PCA and HCA analysis with the standard gallic acid. In the molecular docking study, the compounds studied presented the binding free energy (ΔG) values close each other, due to the similar interactions with amino acids residues at the activity site. The descriptors Gap and softness were important to characterize the molecules with antioxidant potential by capturing oxygen radicals.

scholarly journals Cycloartane-Type Triterpenes and Botanical Origin of Propolis of Stingless Indonesian Bee Tetragonula sapiens

Plants ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 57 ◽  
Author(s):  
Niken Pujirahayu ◽  
Toshisada Suzuki ◽  
Takeshi Katayama
Keyword(s):  
High Performance ◽  
Chemical Constituents ◽  
Mangifera Indica ◽  
Ethanol Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Botanical Origin ◽  
Characteristic Peak ◽  
Hplc Chromatogram ◽  
Main Components ◽  
Layer Chromatography

This study clarifies the chemical constituents and botanical origin of Tetragonula sapiens Cockerell bee propolis collected from Southeast Sulawesi, Indonesia. Propolis samples and resin of Mangifera indica were extracted with 99% ethanol to obtain an ethanol extract of propolis (EEP) and an ethanol extract of M. indica resin (EEM). Column chromatography, thin-layer chromatography (TLC), and high-performance liquid chromatography (HPLC) were developed and used for the separation and isolation of compounds from the ether-soluble fraction. The structure of the compounds was determined by nuclear magnetic resonance (NMR) spectroscopic analysis, and their molecular weight analyzed by gas chromatography–mass spectrometry (GC–MS). The HPLC chromatogram of the EEP was then compared with the HPLC chromatogram of EEM to investigate the botanical origin of propolis. Five compounds were isolated from the EEP, and their structures were determined as mangiferolic acid, cycloartenol, ambonic acid, mangiferonic acid, and ambolic acid, which are cycloartane-type triterpenes. The characteristic peak of the HPLC chromatograms of EEP and EEM showed a similar pattern, which is that the main components of propolis were also found in M. indica resin. These results suggested that the propolis from Southeast Sulawesi was rich in cycloartane-type triterpenes, and the plant source of the propolis could be Mangifera indica (mango).

scholarly journals Triterpenes from Warszewiczia coccinea (Rubiaceae) as Inhibitors of Acetylcholinesterase

2009 ◽  
Vol 4 (10) ◽  
pp. 1934578X0900401
Author(s):  
Angela I. Calderón ◽  
Johayra Simithy ◽  
Giuliana Quaggio ◽  
Alex Espinosa ◽  
Jose Luis López-Pérez ◽  
...  
Keyword(s):  
Free Radical ◽  
Thin Layer ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Spectroscopic Methods ◽  
Ache Inhibitors ◽  
Ache Inhibitory Activity ◽  
Stem Extract ◽  
Layer Chromatography ◽  
Free Radical Scavenging Activities

Seventy-six plant extracts from the Panamanian flora have been screened for acetylcholinesterase (AChE) inhibitors by thin-layer chromatography (TLC) bioautography. The most promising extracts with AChE inhibitory and free radical scavenging activities at 100 μg were those of Tabernaemontana panamensis (Markgr., Boiteau & L. Allorge) Leeuwenb., Pentagonia macrophylla Benth., and Warszewiczia coccinea (Vahl) Klotzsch. Bioguided fractionation of W. coccinea stem extract afforded two triterpenes, 3β,6β,19α-trihydroxy-urs-12-en-28-oic acid (1) and 3β,6β-dihydroxy-olean-12-en-28-oic acid (sumaresinolic acid) (2), with AChE inhibitory activity. Their structures were determined by spectroscopic methods. This is the first report of these bioactive triterpenes in W. coccinea.

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