Kualitas semen segar kambing kacang pada suhu 50c dengan lama simpan yang berbeda menggunakan pengencer dan tanpa pengencer

2017 ◽  
Vol 5 (2) ◽  
pp. 77-85
Author(s):  
Feri Setiawan ◽  
Enike Dwi Kusumawati

Abstrak Penelitian ini bertujuan untuk mengetahui kualitas semen segar Kambing Kacang pejantan pada penyimpanan suhu 5oC dengan lama simpan yang berbeda menggunakan pengencer dan tanpa pengencer. Penelitian ini dilakukan di Laboratorium Fakultas Peternakan Universitas Kanjuruhan Malang pada tanggal 6 sampai 11 Februari 2012. Metode penelitian yang digunakan adalah metode percobaan dengan menggunakan Rancangan Acak Lengkap (RAL) Faktorial. Materi penelitian yang digunakan adalah semen segar Kambing Kacang. Berdasarkan hasil penelitian menunjukkan bahwa kualitas spermatozoa Kambing Kacang pada suhu 5oC dengan lama simpan yang berbeda menggunakan pengencer dan tanpa pengencer menunjukkan adanya perbedaan yang sangat nyata (P<0.01). Pada pengamatan motilitas dan viabilitas spermatozoa kambing Kacang pada suhu 5oC baik yang menggunakan pengencer maupun tanpa pengencer menunjukkan adanya perbedaan yang sangat nyata (P<0.01). Tetapi pada pengamatan abnormalitas tidak menunjukkan adanya pengaruh penggunaan pengencer (P>0.01) serta tidak terdapat pengaruh interaksi antara lama simpan dan penggunaan pengencer terhadap abnormalitas spermatozoa kambing Kacang (P>0.01). Namun terdapat pengaruh yang sangat nyata lama simpan terhadap abnormalitas spermatozoa kambing Kacang pada suhu 5oC baik yang menggunakan pengencer maupun tanpa pengencer (P<0.01). Kesimpulan yang diperoleh dari hasil penelitian ini yaitu  bahwa penyimpanan semen segar kambing Kacang pada suhu 50C tanpa menggunakan pengencer mempunyai kualitas yang paling baik yaitu mampu bertahan sampai lama simpan ke 42 jam.   Abstract  This research aimed to determine the quality of a fresh semen Kacang goat at 50C temperature the various preservings time within and withouth extender.  This research had been performed in Animal Husbandry Faculty of  Kanjuruhan University Malang since 6 until 11 February 2012. The research method used was Completely Randomized Design (CRD) Factorial. Research used was fresh semen Kacang goat. The result of the research indicated that spermatozoa quality of Kacang goat at 50C in different preservings time within and without extender showed the significant differentcy (P<0,01). In motility and viability observation spermatozoa of Kacang goat at 50C either with and without extender showed the significant differntcy  (P<0,01). But at the abnormality observation it did not show the influence of the usage extender (P>0,01) and there was no interaction influence between the preserving time and the extender usage forwards the abnormality of Kacang goat spermatozoa (P>0,01). But there was a significant influence of preserving time to wards the abnormality of spermatozoa at 50C temperature either with extender and withoud extender (P<0,01). This research result concludes that the Kacang goat fresh semen preserving without using extender is the best quality that it is able to stay freshly until 42 hours.

2017 ◽  
Vol 5 (1) ◽  
pp. 50-56 ◽  
Author(s):  
Yanpiterson Umbu Lele ◽  
Enike Dwi Kusumawati ◽  
Aju Tjatur Nugroho Krisnaningsih

ABSTRAK Tujuan penelitian ini adalah untuk mengetahui motilitas dan viabilitas spermatozoa semen hasil sexing kambing Peranakan Etawa (PE) dengan metode sedimentasi putih telur menggunakan pengencer yang berbeda. Materi yang digunakan dalam penelitian ini adalah semen segar kambing Peranakan Etawa (PE) berumur 2 tahun dan bobot badan 120 kg dari Balai Besar Inseminasi Buatan (BBIB)  Singosari Malang. Metode penelitian ini yang digunakan adalah penelitian laboratorium dengan menggunakan rancangan acak lengkap (RAL). Perlakuan terdiri dari semen sexing dengan menggunakan pengencer Andromed lapisan atas dan Andromed lapisan bawah dan Tris Aminomethan Kuning Telur lapisan atas dan Tris Aminomethan Kuning Telur lapisan bawah dengan masing-masing 10 ulangan. Variabel yang diamati adalah motilitas dan viabilitas spermatozoa semen sexing dengan pengencer yang berbeda, proses sexing menggunakan metode sedimentasi putih telur. Data yang diperoleh dianalisis menggunakan Analisis Varian  (ANOVA) apabila perlakuan memberikan perbedaan, maka dilanjutkan dengan Uji BNT. Hasil penelitian menunjukkan bahwa metode sedimentasi putih telur dengan pengencer yang berbeda pada kambing Peranakan Etawa (PE) memberikan pengaruh yang sangat nyata (P<0,01) terhadap motilitas dan viabilitas spermatozoa semen sexing. Motilitas dan viabilitas terbaik terdapat pada pengencer Tris aminomethan kuning telur lapisan atas sebesar 75,65%, 74,41% dan Andromed lapisan atas sebesar 65,4%, 60%. Berdasarkan hasil penelitian dapat disimpulkan bahwa kualitas spermatozoa semen sexing kambing PE dengan menggunakan pengencer Tris aminomethan memberikan hasil terbaik terhadap motilitas dan viabilitas. Berdasarkan hasil penelitian ini maka disarankan agar menggunakan pengencer Tris aminomethan kuning telur sebagai pengencer sexing spermatozoa.   ABSTRACT The purpose of this study was to determine the motility and viability of PE sperm sexing with egg white sedimentation method using different diluents. This research method used was laboratory research using a completely randomized design (CRD) treatment PE sexing sperm. The results showed that the quality of PE sperm goat with various diluents showed a significant influence (P<0,01). The variables observed were motility and viability of sperm. The data obtained were analyzed using variance analysis (ANOVA) with complete randomized design (CRD). Motility and viability at best against Tris aminomethane top layer of egg yolk 65,4% and the top layer of Andromed 75,65%, 74,41%. Based on the results it is suggested that using Tris aminomethane yolk as a diluents sexing sperm. It can be concluded that the quality of sexing sperm goat PE by using tris aminomethane diluent gives the best result on motility and viability.


2018 ◽  
Vol 3 (2) ◽  
pp. 35
Author(s):  
Junianto Wika Adi Pratama ◽  
Dyah Ayu Kartika Sari ◽  
Miarsono Sigit

The study aimed to determine the effect of several temperatures of thawing water and the length of thawing on the quality of Simmental frozen semen. This research method was an experimental study used a multifactorized completely randomized design with treatment of thawing water temperature 26oC, 37oC, 42oC and long thawing 10 seconds, 15 seconds and 20 seconds. The data obtained were analyzed by Analysis of Variance (ANOVA). If there are differences in the real or very significant influence on each treatment, then Duncan's multiple distance test is continued. The results showed that there was a significant effect (P <0.05) between the duration of thawing and thawing temperature on sperm motility and viability, but there was no interaction between temperature and thawing time (P> 0.05). Thawing temperature of 37 ° C and thawing time of 20 seconds showed the highest viability and motility of sperm so that it could provide the best quality of Simmental cow frozen semen for artificial insemination


2020 ◽  
Vol 7 (2) ◽  
pp. 119
Author(s):  
Lukman Affandhy ◽  
Muchamad Luthfi ◽  
Dian Ratnawati ◽  
Frediansyah Firdaus

ABSTRAK  Penelitian bertujuan untuk mengetahui pengaruh pemberian bubuk daun Moringa oleifera (MO) terhadap kuantitas dan kualitas semen sapi peranakan ongole (PO). Metode penelitian menggunakan percobaan lapang dengan rancangan acak lengkap dengan tiga perlakuan pemberian MO sebesar 0 kg/ekor/hari (P1); 0,05 kg/ekor/hari (P2) dan 0,1 kg/ekor/hari (P3). Analsis data menggunakan one way analysis of variance. Masing-masing perlakuan terdiri atas empat ekor dengan tiga periode pengamatan sebagai ulangan, yaitu periode pertama dan ketiga tanpa MO, dan periode kedua diberikan MO pada P2 dan P3. Hasil penelitian menunjukan bahwa volume dan pH semen perlakuan P1, P2 dan P3 tidak berbeda semua periode pengamatan. Motilitas massa spermatozoa P2 dan P3 periode kedua dan ketiga menunjukan nilai +++ berbeda nyata (P<0,05) dibandingkan dengan P1 (++); motilitas individu  perlakuan P1 menunjukkan nilai terendah 60,56±2,94 % (P<0,05) dibandingkan P2 dan P3 pada periode kedua dan ketiga (75,50±2,29 dan 72,50±2,34%). Konsentrasi spermatozoa P2 dan P3 periode kedua dan ketiga menunjukkan rata-rata  >1.350 juta/ml berbeda nyata (P<0,05) daripada P1 (876±152juta/ml), sedangkan total spermatozoa motil menunjukkan nilai tertinggi 5.647±829 juta/ml pada P3 periode kedua.Viabilitas spermatozoa P2 dan P3 menunjukkan nilai >88-89 % beda nyata (P<0,05) daripada P1 (<84 %) pada periode kedua dan ketiga, sedangkan nilai abnormalitas spermatozoa yang terbaik adalah P2 sebesar 4,30% pada periode kedua dan P3 sebesar 5,33% pada periode ketiga. Disimpulkan pemberian bubuk daun MO dengan dosis 0,1 kg/ekor/hari dapat memperbaiki kuantitas dan kualitas semen (viabilitas dan total spermatozoa motil) sapi pejantan PO.Kata kunci:  kuantitas dan kualitas semen, Moringa oleifera, sapi pejantan ABSTRACTThe research aimed to determine the effect of Moringa oleifera (MO) leaf powder on the quantity and quality of semen of ongole crossbreed. The research method used was a field experiment with a completely randomized design with three treatments giving MO of 0 kg/head/day (P1); 0.05 kg/head/day (P2) and 0.1 kg/head/day (P3). Each treatment consisted of four heads with three periods of observation as replications, namely the first and third periods without MO, and the second period was given MO on P2 and P3. Data analysis used a one-way analysis of variance. The results showed that the volume and pH of semen treated P1, P2, and P3 didn’t different in all observation periods. The second and third period spermatozoa mass motility of P2 and P3 showed that the value of +++ was significantly different (P<0.05) compared to P1 (++); Individual motility of P1 treatment showed the lowest value 60,56±2,94 % (P<0.05) compared to P2 and P3 in the second and third periods (75,50±2,29 and 72,50±2,34%). The second and third periods of P2 and P3 spermatozoa concentrations showed an average of >1,350 million/cc significantly different (P<0.05) than P1 (876±152 million/cc), while the total motile spermatozoa showed the highest value of 5,647±829 million/cc in the second period P3. The spermatozoa viability of P2 and P3 showed values >88-89% significantly different (P<0.05) than P1 (<84%) in the second and third periods, while the best spermatozoa abnormalities were P2 at 4.30% in the second period and P3 at 5.33% in the third period. It was concluded that the giving of MO leaf powder at a dose of 0,1 kg/head/day could improve the quantity and quality of semen (viability and total motile spermatozoa) of Ongole Crossbreed bulls. Keywords: bulls, Moringa oleifera, quantity and quality of semen


2021 ◽  
Vol 888 (1) ◽  
pp. 012054
Author(s):  
A Jaelani ◽  
N Abelina ◽  
R Samudera ◽  
T Rostini ◽  
Masganti ◽  
...  

Abstract This research aims to study and analyze the effect of adding turmeric flour (Curcuma domestica Val) in the rations on the performance of 70 weeks old Isa Brown strain of laying hens. The research method used in this study was an experimental method with a completely randomized design (CRD) step with five treatments and four replications. The number of chickens used was as many as 40 laying hens. The treatments given to the sample were P0 = feed without giving turmeric flour (control), P1 = feed with 0.3% turmeric flour, P2 = feed with 0.5% turmeric flour, P3 = feed with 0.7% turmeric flour and treatment P4 = feed by giving turmeric flour 0,9%. The results showed that giving turmeric flour in the rations of the Isa Brown strain of laying hens affected chicken performance and egg quality. This research concludes that giving turmeric flour a percentage of 0.7% in Isa Brown’s 70-week old laying hens is the best for ration consumption, Hen Day Production, egg weight, feed conversion, and egg quality: Haught Unit, yolk egg color, yolk egg index, grading egg and egg mass. Based on the haugh unit value, all eggs produced are still in the AA grade category


2018 ◽  
Vol 1 (1) ◽  
pp. 1-8
Author(s):  
Mohammad Badru Zaman ◽  
Ristika Handarini ◽  
Deden Sudrajat

The torch ginger (Etlingera elatior) is a herbaceous plant the flower of which is commonly used in feed to improve the quality of meat. This study was aimed at assessing the effects of giving commercial feed non-carcass portions of local ducks. The study was conducted at the Poultry Farm of Department of Animal Husbandry Djuanda University, Bogor, from June to August 2016. Twenty four 2-week-old male local ducks were used. Completely randomized design with 4 treatments and 3 replicates was used. The treatments consisted of 100% of commercial ration (R0), commercial ration + 2.5% of TGE solution (R1), commercial ration + 5% of TGE solution (R2), and commercial ration + 7.5% of TGE solution (R3). The feeding trial lasted 6 weeks after which the ducks were slaughtered and carcass weight, dressing percentages and carcass parts were measured. The percentage of non-carcass parts including head, neck, shank, liver, heart, gizzard, feathers, blood and intestines were also measured. No significant effect of treatments (P > 0.05) on all parameters measured were found. It was concluded that the inclusion of TGE solution in commercial ration up to 7.5% did not significantly affect the live weight, weight and percentages of non-carcass parts including blood, feathers, head, neck, shank, liver, gizzard, heart, and intestines.


2020 ◽  
Vol 8 (1) ◽  
pp. 41-56
Author(s):  
Enike Dwi Kusumawati ◽  
Aju Tjatur Nugroho Krisnaningsih ◽  
Aditya Umbu Kulli Walangara

This research was conducted at the Laboratory of the Faculty of Animal Husbandry, University of Kanjuruhan Malang, which began in March until May 2019. The purpose of this study was to study the quality of semen of Kampung Ayam Arabian funds with long stored Arabs that produce in spaces. In this study, the material used was cement of Kampung chickens and Arabic chickens which were requested from two male chickens and 2 male Arab chickens needed 1.5-2 years that had a body weight of ± 2.5 kg and each tail was accommodated 1 time per day and in one shelter done 1 ejaculation. The laboratory test method uses a completely randomized design (factorial). The research treatment is storing time 0, 1, 2, 3, and 4 hours. Each payment is repeated ten times. The data obtained were analyzed using analysis of variance. The conclusion of this study is that there is an effect of shelf life on the quality of spermatozoa. After 4 hours motility is not feasible for IB, after 4 hours the viability is not feasible for all, after 4 hours the abnormality is not feasible for all.


2019 ◽  
Vol 20 (3) ◽  
pp. 397
Author(s):  
Junaedi Junaedi ◽  
Husnaeni Husnaeni

This study was conducted to examine the comparison the quality of spermatozoa in four genetic groups of poultry (Pelung chicken, Nunukan chicken, Sentul chicken, and Bangkok chicken). The parameters of this study were the concentration of spermatozoa, motility, viability, semen volume, color, and consistency. The study was designed using a Completely Randomized Design (CRD) with four local chicken nations and four replications. The results showed that the volume of Nunukan chicken semen was 0.32±0.01 mL/ejaculation, Bangkok chicken was 0.31±0.01 mL/ejaculate, Sentul chicken was 0.15±0.02 mL/ejaculate and Pelung chicken was 0.23±0.02 mL/ejaculation. the color of fresh semen is white (Pelung chicken, Nunukan chicken and Sentul chicken) except chicken Bangkok has the color of cream semen. Pelung chicken has a concentration of spermatozoa 5,043.33±51 million/mL, Nunukan chicken 3,250.22 ±45 million/mL, Sentul chicken 3,002.87±67 million/mL, and Bangkok chicken 3,002.87 ± 67 million/mL. Motility of Pelung chicken 84.,69 ± 1.12%, Bangkok chicken 82.35±1.85%, Nunukan chicken 77.74±1.57% and Sentul chicken 77.64±1.65%. Viability spermatozoa of Sentul chicken was 90.35 ± 1.21%, Bangkok chicken was 90.64 ± 1.16%, Pelung chicken was 89.17 ± 1.23% and Nunukan chicken was 86.29 ± 1.15%. It can be concluded that the motility spermatozoa of Pelung chicken and Bangkok chicken is better than the spermatozoa motility of Nunukan chicken and Sentul chicken. The viability of Sentul chicken and Bangkok chicken was higher compared to the viability semen of chicken Pelung and chicken Nunukan.


2017 ◽  
Vol 5 (1) ◽  
pp. 1 ◽  
Author(s):  
Riawan Riawan ◽  
Riyanti Riyanti ◽  
Khaira Nova

The purpose of this research was to find out interior quality of egg laying hens which immersion with moringa leaf solution and to find out the best moringa leaf solution.  This research carried out on August 14--September 13, 2016 housed in the Laboratory Animal Production and Reproduction, Department of Animal Husbandry, Faculty of Agriculture, University of Lampung.  The material of research used 72 eggs laying hens strain isa brown from layer of 60 weeks old with the average weight 63,0±1,51 gram and coefficient of variation 2,40%.  This research used a Completely Randomized Design with 4 treatments and 6 repetition.  The treatments of research consists of immersion egg used moringa leaf solution 0% (w/v), 10% (w/v), 20% (w/v), and 30% (w/v).  Analyzed data observation used variance with 5% trust level and continued with Least Significant Different test.  The result showed that immersion egg with moringa leaf solution significant effect (P<0,05) increase albumin index and the haugh unit, and not significant effect (P>0,05) to yolk index and percentage egg weight lo.  Concentration 30% of immersion moringa leaf solution to give the best treatment to interior quality of egg laying hens.                                 Keywords :  Albumin Index Moringa Leaf, Percentage Egg Weight Lost, Yolk Index, Haugh Unit.


2019 ◽  
Vol 6 (1) ◽  
pp. 119
Author(s):  
Enike Dwi Kusumawati ◽  
Syam Rahadi ◽  
Sugeng Santoso ◽  
Dyah Lestari Yulianti

ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh lama thawing yang berbeda pada suhu 250C untuk mendapatkan kualitas spermatozoa semen beku sapi ongole yang optimal. Penelitian dilakukan di Laboratorium Fakultas Peternakan Universitas Kanjuruhan Malang. Metode Penelitian yang digunakan adalah metode percobaan dengan menggunakan Rancangan Acak Lengkap (RAL) Faktorial. Setiap perlakuan thawing diberikan ulangan sebanyak 10 sampel semen beku sapi ongole. Hasil penelitian menunjukkan bahwa perlakuan dengan lama thawing 7, 15, 30 detik pada suhu 25oC memberikan perbedaan pengaruh yang sangat nyata (p<0.01) pada motilitas, viabilitas dan abnormalitas spermatozoa semen beku sapi ongole. Motilitas tertinggi diperoleh pada perlakuan P3 dengan rata-rata sebesar 40,8%, viabilitas tertinggi diperoleh pada perlakuan P3 dengan rata-rata sebesar 82,39%, sedangkan abnormalitas terendah diperoleh pada perlakuan P3 dengan rata-rata sebesar 11,95%. Berdasarkan penelitian ini maka dapat disimpulkan bahwa thawing pada suhu 25oC dengan lama waktu 30 detik memberikan kualitas spermatozoa yang paling baik sehingga disarankan untuk melakukan thawing pada suhu 25oC dengan lama waktu 30 detik. Kata kunci: abnormalias, mortalitas, ongole, thawing, viabilitas ABSTRACT This study aims to determine the effect of different thawing time at 250C to get the sperm quality of frozen semen ongole bull. Research conducted at the Laboratory of Animal Husbandry Faculty Kanjuruhan University of Malang. The method of study by using Completely Randomized Design (CRD) factorial. Any treatment given repeated thawing of frozen samples of 10 times. The study show that treatment with time thawing 7 (P1), 15 (P2), 30 (P3) seconds at 25oC gives a very significant difference (P<0.01) on motility, viability and abnormalities of ongole Bull sperm. The highest motility and viability were obtained at P3 (40,8%) and (82,39%), while the lowest abnormalities obtained on P3 (11,95%). Based on this research it can be concluded that thawing at 25oC with 30 seconds to give the best quality sperm that is recommended for thawing. Keywords: abnormality, motility, ongole, thawing, viabilit


Pastura ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 69
Author(s):  
Montesqrit Montesqrit ◽  
Harnentis Harnentis ◽  
Sri Yana

This study aims to evaluate how the effect of the addition level of neem leaf flour (Melia azedarach Linn) on the quality of shelled corn during storage. The research material used was neem leaves, shelled corn, storage containers and analytical equipment. Neem leaves are given in the form of flour with a size of 60 mesh while corn used in shell corn with water content and aflatoxin content before storage are 20% and 110 ppb respectively. Shelled corn was used as much as 1 kg for each treatment and stored in modified woven bags for 4 weeks. The research method is an experimental method using a completely randomized design (CRD), with 4 treatments and 4 replications. The treatments were the percentage of neem leaf flour addition in shelled corn, the four treatments were treatment A (0% neem leaf flour), B (2.5% neem leaf flour), C (5% neem leaf flour), and D ( 7.5% neem leaf flour). Parameters measured were percentage of damaged seeds, percentage of moldy seeds, moisture content and aflatoxin content. The results of the study showed that the treatment had a highly significant effect (P<0.01) on% of damaged seeds, % of moldy seeds, and aflatoxin content on shelled corn during storage. The administration of neem leaf flour at 2.5% level was able to maintain the quality of shelled corn during storage, where the aflatoxin content could decrease from 110 ppb before storage to 34,25 ppb after being stored for 4 weeks. Keywords: neem (Melia azedarach Linn), shelled corn, storage, aflatoxin


Sign in / Sign up

Export Citation Format

Share Document